Rw0G011130

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00481
OG0016402
Physical Location & Seq
Reverse (-)
32462 .. 34625
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011130.1

Sequence Viewer

Length: 2133 bp
ATGGATAAAATTAGGGAGATACCATTTTTCTCCAAAGAGGTGAAGAAGATATGGGACGTGTGGAATCTGCAGGGCTGTATTCTCTTAAGTCTCTCACTGCAAGTTTCTCTGCTCTTGGCATCATTTCGGAAAAAATGCAGAAGCAAATTGCTACGAGGACTCATCTGGTCTGCGTATTTGCTCGCCGATTGGATTGCTGCTGTTGCAATAGGGATAATCACCAAATCCCAAGTTGACACTTGCGACCCGAAGGGCAACAAAGATCTTTTGGCATTCTGGGCTTCCTTTCTTCTACTCCATCTTGGCGGCCCTGATACCATTACCTCTTTTGACAATGAGTTCTGGGTTAGGCACTTGGTTGGACTAATTGTTCAAGTTCTTGCAGCTGCTTATTGTTTCTACCTGACACTTGTTCATAACAAGTTCTTGGTTCCGACGGTGCTAGTCTTTGTTGTTGGAACAATCAAGTTTGGGGAGAGAACTTGTGCTCTGTATCTTGCAAGTATAGACCATTTTGGGGAGACTGTATTGCCAAAGCCGGTGGCCGGACCTGACTATGAAGAAACTGTAGCAATCCACACTTCAATGAGGTCAGTTGAAGTCCCTACAACACAAGAAGTAGTGGTGTCAATCAGGCCATGTATTGGGGATGCTCATAAAGATCCCAAGTTTACAGTCAAAGGAAATAAAAAAGAATCTTTAAATGACATGCAATTGCTGCAAGAAGCATATTACCTCTTTGAGAGCTTCAAAAGACTCATTGTTGGCTTCCTTCTCAGCTCCAAAGACCGAGAACTGAGTCGAGATTTCTTCCTTAAAAGAACCTATACTAGCGCTTTTCAGTTGATAGAGTACGAGCTCAGCTTCATGTATGAGGTTCTTCACACCAAGGTTATTGTGGTGCGTCGCAAAATTGGGTACACTATCCGCTTAGTAAGCTTCTGTGCAACCATTGTTGCCTTGGTGTTCTTCTTTTCAGTTGAAAAGATAGAGTCTGCTAAGTTTGACATTGATCTAACTTATGCTTTGTTTAGTGGAGCCATTTTCCTGGACATCATATCTGTCTTTACGCTTATCTTCTCTGACTGGACCCTCATTGCCTTCAACAACACTAACATTTGGAGCAGATTTATTGCATCTGTAATAATGAAAGGAGGAAGGTGGTCTGGATCGGTATCCCAATACAATATGATAAAGAATTGCCTGGATGAGCGTCCAGAATGTGTCTTGACTCTTGCCGGTTATCTGGGTGTTGGGGGAATTCTAGGCAGAACCAAAATTGTCTACTTTTCATCCTCAGAAGCAGTCACCGAAGAGCTGAAGTACTTTATCTTCAGGCAGCTGAGGTTAAAATCTCTGAAAGCAAACAACAGAATGGATGGAATGGAGGGATGTTCACAAAGAGGTGACTGGGCTCTGCTGCAGAATTCAAAGTATCAGAAACTCAGATGGAGCATTGGGGATTATCAATATGCGGAAAGTGTTCTTCTCTGGCACATTGCAACAGAACTCTACTATGCTGAAAGTGGAACCGAGCAATGTACTGATTGTGGTCAAGGTAGACAAGCAACACAAAGGGAATGTGATAGTGACAATCGACTAAAACTTTGCAATTTTTGTGAAAGTAACGCCGGGCGTAGTATTTGCAAACTTGTTTCAGATTATCTGTTTTATCTTCTGGTGATGCAGCATCCAATGATGGCCCCAGTTTTGGGGAATTGGCATATAGTGTTCCAGGACACTTGTGCAGAGGCCAAGAGATTCTTTCGCAAGCATTCAGTAAAAGATCATAAGGAAGCCTGTAAAAAGATTTCTGATGTCAAAGCCCAATTCAGATCGGCTGCTGTGAAGGGAAGTAAAAGCAAATCTGTGCTGTTCGATGCATGTATACTTGCAGAACAGCTTAAGGAGTTGGAGGCAGAGCAGGACCCATGGGAGCTAATGAGCCGGGTGTGGGTGGAATTATTGTCATATGCTTCCATTAAATGTAGACCGATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTGATGACTTTCACTTGGCTACTGATGAATCATCTGGGCTTGGGAACACAATTCTACGAGCTGGAAAACCAAGCTGGAACAAAAATGGTGGCAGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

710

Amino Acids

80.92

Weight (kDa)

8.57

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 56 - 399 2e-89 Domain of unknown function (DUF4220)
DUF594 PF04578 639 - 691 4.5e-18 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 4 cut(s) 1284, 1561, 1888, 1990
AciI CCGC 3 cut(s) 306, 928, 1475
AclWI GGATC 2 cut(s) 656, 1177
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 2 cut(s) 1260, 1426
AcuI CTGAAG 2 cut(s) 1318, 1340
AfaI GTAC 5 cut(s) 854, 920, 1325, 1543, 2001
AfeI AGCGCT 1 cut(s) 835
AfiI CCNNNNNNNGG 7 cut(s) 517, 545, 644, 1711, 1712, 1954, 2021
AflII CTTAAG 2 cut(s) 85, 1904
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 7 cut(s) 374, 585, 599, 751, 983, 1105, 1431
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 3 cut(s) 1047, 1203, 1734
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
Alw21I GWGCWC 2 cut(s) 490, 861
Alw26I GTCTC 2 cut(s) 95, 515
AlwI GGATC 2 cut(s) 656, 1177
Aor51HI AGCGCT 1 cut(s) 835
AoxI GGCC 5 cut(s) 307, 543, 635, 1701, 1752
ApeKI GCWGC 8 cut(s) 197, 383, 386, 718, 1339, 1420, 1687, 1841
ApoI RAATTY 2 cut(s) 1260, 1426
ArsI GACNNNNNNTTYG 2 cut(s) 1590, 1622
Asp700I GAANNNNTTC 1 cut(s) 1482
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 5 cut(s) 308, 548, 1089, 1702, 1927
AsuC2I CCSGG 2 cut(s) 1633, 1949
AsuHPI GGTGA 5 cut(s) 52, 211, 1300, 1418, 1693
AvaII GGWCC 3 cut(s) 548, 1089, 1927
BanII GRGCYC 2 cut(s) 861, 1417
BarI GAAGNNNNNNTAC 2 cut(s) 1316, 1348
Bbv12I GWGCWC 2 cut(s) 490, 861
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 8 cut(s) 184, 373, 395, 705, 1351, 1407, 1699, 1828
BccI CCATC 4 cut(s) 306, 1373, 1443, 1693
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 3 cut(s) 1049, 1205, 1736
BciVI GTATCC 1 cut(s) 1186
BcnI CCSGG 2 cut(s) 1633, 1949
BcoDI GTCTC 2 cut(s) 95, 515
BfaI CTAG 3 cut(s) 443, 831, 1265
BfmI CTRYAG 3 cut(s) 68, 567, 1421
BfoI RGCGCY 1 cut(s) 837
BfrI CTTAAG 2 cut(s) 85, 1904
BfuI GTATCC 1 cut(s) 1186
BglII AGATCT 1 cut(s) 262
BisI GCNGC 9 cut(s) 198, 307, 384, 387, 719, 1340, 1421, 1688, 1842
BlpI GCTNAGC 2 cut(s) 860, 2007
BlsI GCNGC 9 cut(s) 199, 308, 385, 388, 720, 1341, 1422, 1689, 1843
BmcAI AGTACT 1 cut(s) 1325
Bme1390I CCNGG 5 cut(s) 1049, 1205, 1633, 1736, 1949
Bme18I GGWCC 3 cut(s) 548, 1089, 1927
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 5 cut(s) 308, 548, 1089, 1702, 1927
BmiI GGNNCC 6 cut(s) 432, 1039, 1091, 1531, 1704, 1929
BmrFI CCNGG 5 cut(s) 1049, 1205, 1633, 1736, 1949
BmrI ACTGGG 2 cut(s) 1420, 1700
BmsI GCATC 6 cut(s) 128, 640, 1145, 1674, 1699, 1870
BmuI ACTGGG 2 cut(s) 1420, 1700
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu1102I GCTNAGC 2 cut(s) 860, 2007
BpuMI CCSGG 2 cut(s) 1633, 1949
BsaBI GATNNNNATC 1 cut(s) 1174
BsaJI CCNNGG 3 cut(s) 888, 960, 1931
Bsc4I CCNNNNNNNGG 7 cut(s) 517, 545, 644, 1711, 1712, 1954, 2021
Bse118I RCCGGY 2 cut(s) 538, 1238
Bse1I ACTGG 3 cut(s) 1091, 1415, 1706
Bse3DI GCAATG 3 cut(s) 1095, 1497, 1544
Bse8I GATNNNNATC 1 cut(s) 1174
BseBI CCWGG 3 cut(s) 1049, 1205, 1736
BseDI CCNNGG 3 cut(s) 888, 960, 1931
BseGI GGATG 6 cut(s) 655, 1213, 1292, 1384, 1397, 1690
BseJI GATNNNNATC 1 cut(s) 1174
BseLI CCNNNNNNNGG 7 cut(s) 517, 545, 644, 1711, 1712, 1954, 2021
BseMI GCAATG 3 cut(s) 1095, 1497, 1544
BseMII CTCAG 7 cut(s) 788, 790, 874, 1311, 1334, 1459, 2021
BseNI ACTGG 3 cut(s) 1091, 1415, 1706
BseXI GCAGC 8 cut(s) 184, 373, 395, 705, 1351, 1407, 1699, 1828
BsgI GTGCAG 1 cut(s) 1767
BshFI GGCC 5 cut(s) 309, 545, 637, 1703, 1754
BsiHKAI GWGCWC 2 cut(s) 490, 861
BsiSI CCGG 5 cut(s) 539, 546, 1239, 1632, 1948
BslFI GGGAC 2 cut(s) 68, 587
BslI CCNNNNNNNGG 7 cut(s) 517, 545, 644, 1711, 1712, 1954, 2021
BsmAI GTCTC 2 cut(s) 95, 515
BsmFI GGGAC 2 cut(s) 68, 587
BsmI GAATGC 2 cut(s) 272, 1774
BsnI GGCC 5 cut(s) 309, 545, 637, 1703, 1754
Bsp1286I GDGCHC 3 cut(s) 490, 861, 1417
Bsp1407I TGTACA 1 cut(s) 1999
Bsp143I GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
Bsp1720I GCTNAGC 2 cut(s) 860, 2007
Bsp19I CCATGG 1 cut(s) 1931
BspACI CCGC 3 cut(s) 306, 928, 1475
BspANI GGCC 5 cut(s) 309, 545, 637, 1703, 1754
BspCNI CTCAG 7 cut(s) 789, 789, 873, 1310, 1335, 1458, 2020
BspLI GGNNCC 6 cut(s) 432, 1039, 1091, 1531, 1704, 1929
BspMAI CTGCAG 2 cut(s) 72, 1425
BspPI GGATC 2 cut(s) 656, 1177
BspQI GCTCTTC 1 cut(s) 1308
BspTI CTTAAG 2 cut(s) 85, 1904
BsrDI GCAATG 3 cut(s) 1095, 1497, 1544
BsrFI RCCGGY 2 cut(s) 538, 1238
BsrGI TGTACA 1 cut(s) 1999
BsrI ACTGG 3 cut(s) 1091, 1415, 1706
BssAI RCCGGY 2 cut(s) 538, 1238
BssECI CCNNGG 3 cut(s) 888, 960, 1931
BssMI GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
BssNAI GTATAC 1 cut(s) 1889
BssT1I CCWWGG 3 cut(s) 888, 960, 1931
Bst1107I GTATAC 1 cut(s) 1889
Bst2UI CCWGG 3 cut(s) 1049, 1205, 1736
Bst4CI ACNGT 4 cut(s) 439, 526, 568, 676
Bst6I CTCTTC 1 cut(s) 1308
BstAFI CTTAAG 2 cut(s) 85, 1904
BstAPI GCANNNNNTGC 1 cut(s) 718
BstAUI TGTACA 1 cut(s) 1999
BstC8I GCNNGC 2 cut(s) 183, 1772
BstDEI CTNAG 9 cut(s) 776, 797, 860, 931, 999, 1297, 1343, 1445, 2007
BstDSI CCRYGG 1 cut(s) 1931
BstF5I GGATG 6 cut(s) 655, 1213, 1292, 1384, 1397, 1690
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 6 cut(s) 265, 664, 1015, 1172, 1789, 1838
BstMAI GTCTC 2 cut(s) 95, 515
BstMBI GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
BstMWI GCNNNNNNNGC 4 cut(s) 203, 278, 718, 936
BstNI CCWGG 3 cut(s) 1049, 1205, 1736
BstNSI RCATGY 3 cut(s) 712, 1887, 2006
BstSCI CCNGG 5 cut(s) 1047, 1203, 1631, 1734, 1947
BstSFI CTRYAG 3 cut(s) 68, 567, 1421
BstV1I GCAGC 8 cut(s) 184, 373, 395, 705, 1351, 1407, 1699, 1828
BstX2I RGATCY 2 cut(s) 262, 661
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 2 cut(s) 262, 661
BstZ17I GTATAC 1 cut(s) 1889
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 5 cut(s) 309, 545, 637, 1703, 1754
BtgI CCRYGG 1 cut(s) 1931
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 6 cut(s) 655, 1213, 1292, 1384, 1397, 1690
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 183, 1772
CfoI GCGC 1 cut(s) 836
Cfr10I RCCGGY 2 cut(s) 538, 1238
Cfr13I GGNCC 5 cut(s) 308, 548, 1089, 1702, 1927
CseI GACGC 2 cut(s) 893, 1202
Csp6I GTAC 5 cut(s) 853, 919, 1324, 1542, 2000
CspCI CAANNNNNGTGG 3 cut(s) 522, 557, 2100
CviAII CATG 6 cut(s) 639, 709, 868, 1884, 1932, 2003
CviQI GTAC 5 cut(s) 853, 919, 1324, 1542, 2000
DdeI CTNAG 9 cut(s) 776, 797, 860, 931, 999, 1297, 1343, 1445, 2007
DpnI GATC 6 cut(s) 264, 663, 1014, 1171, 1788, 1837
DpnII GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
DraI TTTAAA 1 cut(s) 702
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 1 cut(s) 1308
EarI CTCTTC 1 cut(s) 1308
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 3 cut(s) 888, 960, 1931
Eco24I GRGCYC 2 cut(s) 861, 1417
Eco47I GGWCC 3 cut(s) 548, 1089, 1927
Eco47III AGCGCT 1 cut(s) 835
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 2 cut(s) 1318, 1340
EcoICRI GAGCTC 1 cut(s) 859
EcoO109I RGGNCCY 1 cut(s) 1927
EcoRI GAATTC 2 cut(s) 1260, 1426
EcoRII CCWGG 3 cut(s) 1047, 1203, 1734
EcoT14I CCWWGG 3 cut(s) 888, 960, 1931
EcoT22I ATGCAT 1 cut(s) 1885
EcoT38I GRGCYC 2 cut(s) 861, 1417
ErhI CCWWGG 3 cut(s) 888, 960, 1931
FaeI CATG 6 cut(s) 642, 712, 871, 1887, 1935, 2006
FalI AAGNNNNNCTT 2 cut(s) 1748, 1780
FaqI GGGAC 2 cut(s) 68, 587
FatI CATG 6 cut(s) 638, 708, 867, 1883, 1931, 2002
FauNDI CATATG 1 cut(s) 1972
FblI GTMKAC 4 cut(s) 1284, 1561, 1888, 1990
Fnu4HI GCNGC 9 cut(s) 198, 307, 384, 387, 719, 1340, 1421, 1688, 1842
FokI GGATG 6 cut(s) 662, 1220, 1279, 1391, 1404, 1677
FriOI GRGCYC 2 cut(s) 861, 1417
Fsp4HI GCNGC 9 cut(s) 198, 307, 384, 387, 719, 1340, 1421, 1688, 1842
FspBI CTAG 3 cut(s) 443, 831, 1265
GlaI GCGC 1 cut(s) 835
GluI GCNGC 9 cut(s) 198, 307, 384, 387, 719, 1340, 1421, 1688, 1842
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 5 cut(s) 309, 545, 637, 1703, 1754
HapII CCGG 5 cut(s) 539, 546, 1239, 1632, 1948
HgaI GACGC 2 cut(s) 893, 1202
HhaI GCGC 1 cut(s) 836
Hin1II CATG 6 cut(s) 642, 712, 871, 1887, 1935, 2006
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 937
HinfI GANTC 9 cut(s) 64, 159, 695, 756, 799, 992, 1231, 1761, 2059
HpaII CCGG 5 cut(s) 539, 546, 1239, 1632, 1948
HphI GGTGA 5 cut(s) 52, 211, 1300, 1418, 1693
Hpy166II GTNNAC 8 cut(s) 235, 672, 921, 1285, 1397, 1562, 1889, 1991
Hpy188III TCNNGA 4 cut(s) 803, 1167, 1217, 1228
Hpy8I GTNNAC 8 cut(s) 235, 672, 921, 1285, 1397, 1562, 1889, 1991
Hpy99I CGWCG 2 cut(s) 439, 909
HpyAV CCTTC 5 cut(s) 244, 782, 1111, 1152, 1843
HpyCH4III ACNGT 4 cut(s) 439, 526, 568, 676
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 278, 718, 936
HpyF3I CTNAG 9 cut(s) 776, 797, 860, 931, 999, 1297, 1343, 1445, 2007
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 6 cut(s) 642, 712, 871, 1887, 1935, 2006
HspAI GCGC 1 cut(s) 834
Kzo9I GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 5 cut(s) 785, 1037, 1122, 1452, 1936
Lsp1109I GCAGC 8 cut(s) 184, 373, 395, 705, 1351, 1407, 1699, 1828
LweI GCATC 6 cut(s) 128, 640, 1145, 1674, 1699, 1870
MaeI CTAG 3 cut(s) 443, 831, 1265
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 4 cut(s) 1306, 1406, 1589, 1625
MalI GATC 6 cut(s) 264, 663, 1014, 1171, 1788, 1837
MboI GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 2 cut(s) 262, 661
MhlI GDGCHC 3 cut(s) 490, 861, 1417
MlyI GAGTC 5 cut(s) 153, 750, 808, 1001, 1225
MmeI TCCRAC 4 cut(s) 340, 436, 458, 1893
Mph1103I ATGCAT 1 cut(s) 1885
MroXI GAANNNNTTC 1 cut(s) 1482
MseI TTAA 6 cut(s) 86, 701, 816, 1349, 1905, 1983
MslI CAYNNNNRTG 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 386, 1342
MspCI CTTAAG 2 cut(s) 85, 1904
MspI CCGG 5 cut(s) 539, 546, 1239, 1632, 1948
MspR9I CCNGG 5 cut(s) 1049, 1205, 1633, 1736, 1949
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 2 cut(s) 272, 1774
MvaI CCWGG 3 cut(s) 1049, 1205, 1736
MwoI GCNNNNNNNGC 4 cut(s) 203, 278, 718, 936
NciI CCSGG 2 cut(s) 1633, 1949
NcoI CCATGG 1 cut(s) 1931
NdeI CATATG 1 cut(s) 1972
NdeII GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
NlaIII CATG 6 cut(s) 642, 712, 871, 1887, 1935, 2006
NlaIV GGNNCC 6 cut(s) 432, 1039, 1091, 1531, 1704, 1929
NmuCI GTSAC 3 cut(s) 1306, 1406, 1589
NsiI ATGCAT 1 cut(s) 1885
NspI RCATGY 3 cut(s) 712, 1887, 2006
PciSI GCTCTTC 1 cut(s) 1308
PctI GAATGC 2 cut(s) 272, 1774
PdmI GAANNNNTTC 1 cut(s) 1482
PfeI GAWTC 4 cut(s) 64, 695, 1761, 2059
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 2 cut(s) 1047, 1734
PkrI GCNGC 9 cut(s) 199, 308, 385, 388, 720, 1341, 1422, 1689, 1843
PleI GAGTC 5 cut(s) 153, 750, 807, 1000, 1225
PpsI GAGTC 5 cut(s) 153, 750, 807, 1000, 1225
PpuMI RGGWCCY 1 cut(s) 1927
Psp124BI GAGCTC 1 cut(s) 861
Psp5II RGGWCCY 1 cut(s) 1927
Psp6I CCWGG 3 cut(s) 1047, 1203, 1734
PspGI CCWGG 3 cut(s) 1047, 1203, 1734
PspN4I GGNNCC 6 cut(s) 432, 1039, 1091, 1531, 1704, 1929
PspPI GGNCC 5 cut(s) 308, 548, 1089, 1702, 1927
PspPPI RGGWCCY 1 cut(s) 1927
PstI CTGCAG 2 cut(s) 72, 1425
PsuI RGATCY 2 cut(s) 262, 661
PvuII CAGCTG 2 cut(s) 386, 1342
RsaI GTAC 5 cut(s) 854, 920, 1325, 1543, 2001
RsaNI GTAC 5 cut(s) 853, 919, 1324, 1542, 2000
RseI CAYNNNNRTG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 6 cut(s) 86, 701, 816, 1349, 1905, 1983
SatI GCNGC 9 cut(s) 198, 307, 384, 387, 719, 1340, 1421, 1688, 1842
Sau3AI GATC 6 cut(s) 262, 661, 1012, 1169, 1786, 1835
Sau96I GGNCC 5 cut(s) 308, 548, 1089, 1702, 1927
ScaI AGTACT 1 cut(s) 1325
SchI GAGTC 5 cut(s) 153, 750, 808, 1001, 1225
ScrFI CCNGG 5 cut(s) 1049, 1205, 1633, 1736, 1949
SduI GDGCHC 3 cut(s) 490, 861, 1417
SfaNI GCATC 6 cut(s) 128, 640, 1145, 1674, 1699, 1870
SfcI CTRYAG 3 cut(s) 68, 567, 1421
SinI GGWCC 3 cut(s) 548, 1089, 1927
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 85, 1904
SmoI CTYRAG 2 cut(s) 85, 1904
SsiI CCGC 3 cut(s) 306, 928, 1475
SspMI CTAG 3 cut(s) 443, 831, 1265
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 5 cut(s) 1047, 1203, 1631, 1734, 1947
StyI CCWWGG 3 cut(s) 888, 960, 1931
TaaI ACNGT 4 cut(s) 439, 526, 568, 676
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 802, 1597, 1878
TaqII GACCGA 2 cut(s) 804, 2008
TatI WGTACW 3 cut(s) 1323, 1541, 1999
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 4 cut(s) 64, 695, 1761, 2059
Tru1I TTAA 6 cut(s) 86, 701, 816, 1349, 1905, 1983
Tru9I TTAA 6 cut(s) 86, 701, 816, 1349, 1905, 1983
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 3 cut(s) 1306, 1406, 1589
TseI GCWGC 8 cut(s) 197, 383, 386, 718, 1339, 1420, 1687, 1841
Tsp45I GTSAC 3 cut(s) 1306, 1406, 1589
TspDTI ATGAA 6 cut(s) 404, 573, 856, 1163, 1281, 2072
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 644
Vha464I CTTAAG 2 cut(s) 85, 1904
VpaK11BI GGWCC 3 cut(s) 548, 1089, 1927
XapI RAATTY 2 cut(s) 1260, 1426
XceI RCATGY 3 cut(s) 712, 1887, 2006
XcmI CCANNNNNNNNNTGG 2 cut(s) 895, 958
XmiI GTMKAC 4 cut(s) 1284, 1561, 1888, 1990
XmnI GAANNNNTTC 1 cut(s) 1482
XspI CTAG 3 cut(s) 443, 831, 1265
ZrmI AGTACT 1 cut(s) 1325
Zsp2I ATGCAT 1 cut(s) 1885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.