Rh3CG233800

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0016402
Physical Location & Seq
Reverse (-)
22115626 .. 22118878
3253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG233800.1

Sequence Viewer

Length: 2136 bp
ATGGGTAAAACTATGGAGCTACCTATACCCAGAGAGGTGAAGAAGATATGGGACGTGTGGAATCTGCGGGGCTGTATTCTCTTAAGTCTCTCCCTGCAAGTTTCTCTGCTTTTGGCATCATTTCGGAAAAAATGCAGAAGCAAATTGCTACGAGGACTCATCTGGTCTGCGTATTTGCTCGCCGATTGGATTGCTGCTGTTGCAATAGGGATAATCACCAAATCCCAAGTTGACACTTGCGATCCGAAGGACAACAAAGATCTTTTGGCATTCTGGGCTTCCTTTCTTCTACTCCATCTTGGCGGCCCTGATACCATTACCTCTTTTGCTCTTGAGGACAATGAGTTCTGGGTTAGGCACTTGGTTGGACTAATTGTTCAAGTTCTTGCAGCTGCTTATTGTTTCTACCTGACACTTGTTCATAACAAGTTCTTGGTTCCGACGGTGCTAGTCTTTGTTGTTGGAACAATCAAGTTTGGGGAGAGAACTTGTGCTCTGTATCTTGCAAGTATAGACCATTTTGGGGAGACTGTATTGCCAAAGCCGGTGGCCGGACCTGACTATGAAGAAACTGTAGCAATCCACACTTCAATGAGGTCAGTTGAAGTCCCTACAACACAAGAAGTAGTGGTGTCAATCAGGCCATGTATTGGGGATGCTCATAAAGATCCCAAGTTTACAGTCAAAGGAAATAAAAAAGAATCTTTAAATGACATGCAATTGCTGCAAGAAGCATATTACCTCTTTGAGAGCTTCAAAAGACTCATTGTTGGCTTCCTTCTCAGCTCCAAAGACCGAGAACTGAGTCGAGATTTCTTCCTTAAAAGAACCTATACTAGCGCTTTTCAGTTGATAGAGTACGAGCTCAGCTTCATGTATGAGGTTCTTCACACCAAGGTTATTGTGGTGCGTCGCAAAATTGGGTACACTATCCGCTTAGTAAGCTTCTGTGCAACCATTGTTGCCTTGGTGTTCTTCTTTTCAGTTGAAAAGATAGAGTCTGCTAAGTTTGACATTGATCTAACTTATGCTTTGTTTAGTGGAGCCATTTTCCTGGACATCATATCTGTCTTTACGCTTATCTTCTCTGACTGGACCCTCATTGCCTTCAACAACACTAACATTTGGAGCAGATTTATTGCATCTGTAATAATGAAAGGAGGAAGGTGGTCTGGATCGGTATCCCAATACAATATGATAAAGAATTGCCTGGATGAGCGTCCAGAATGGGTCTTTACTCTTGCCGGTTATCTGGGTGTTGGGGGAATTCTGGGCAGAACCAAAATTGTCTACTTTTCATCCTCAGAAGCAGTCACCGAAGAGCTCAAGTACTTTATCTTCAGGCAGCTGAGGTTAAAATCTCTGAAAGCAAACAACAGAAGGGATGGAATGGAGGCATGTTCACAAAGAGGTGACTGGGCTCTGCTGCTGAATTTAAACTATCAGAAACTCAAATGGAGCATTGGGGATTATCAATATGCGGAAAGTGTTCTTCTCTGGCACATTGCAACAGAACTCTACCATGCCAGAAGTGGAACCAAGCAATGTGCTCATAGATATGGTAAACAAGGAACACAAAGGGAATGTGATAGTGACGATGAACTAACACTTTGCAAGTCCTGTGAAAGTAACGCTAGGAGTAGTATTTGCAAACTTGTTTCAGATTATCTGTTTTATCTTCTGGTGATGCAGCATCCAATGATGGCCCCAGTTTTGGGGAATTGGCATATAGTGTTCCAGGACACTTGTGCAGAGGCCAAGAGATTCTTTCGCAAGCATTCAGTAAAAGATCATAAGGAAGCCTGTGAAAAAATTTCTGGTGTCAAAGCCAAATTCAGATCGGCTGCTGTGAAAGGAAGTAAAAGCAAATCTGTGCTGTTTGATGCATGTATGCTTGCAGAACAGCTTAAGAAGTTGGAGGCAGATCATGACCCATGGAAGCTAATGAGCCGGGTGTGGGTGGAATTATTGTCATATGCTTCCATTAAATGTAGACCGATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTGATGACTTTCACTTGGCTACTGATGAATCATCTGGGCTTGGGAACACAATTCTACGAGCTGGAAAACCAAGCTGGAACAAAAATGGTGGCAGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

711

Amino Acids

81.08

Weight (kDa)

8.92

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 401 9.7e-94 Domain of unknown function (DUF4220)
DUF594 PF04578 642 - 692 1.5e-17 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 2 cut(s) 1290, 1993
AciI CCGC 4 cut(s) 67, 303, 934, 1481
AclWI GGATC 3 cut(s) 236, 662, 1183
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 4 cut(s) 1266, 1432, 1812, 1832
AcuI CTGAAG 1 cut(s) 1324
AfaI GTAC 4 cut(s) 860, 926, 1331, 2004
AfeI AGCGCT 1 cut(s) 841
AfiI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1714, 1715, 1957, 2024
AflII CTTAAG 2 cut(s) 82, 1907
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 6 cut(s) 380, 591, 605, 757, 989, 1111
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 3 cut(s) 1053, 1209, 1737
AloI GAACNNNNNNTCC 4 cut(s) 329, 360, 361, 392
Alw21I GWGCWC 4 cut(s) 496, 867, 1326, 1552
Alw26I GTCTC 2 cut(s) 92, 521
AlwI GGATC 3 cut(s) 236, 662, 1183
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 5 cut(s) 304, 549, 641, 1704, 1755
ApeKI GCWGC 8 cut(s) 194, 389, 392, 724, 1345, 1426, 1690, 1844
ApoI RAATTY 4 cut(s) 1266, 1432, 1812, 1832
Asp700I GAANNNNTTC 1 cut(s) 1488
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 4 cut(s) 305, 554, 1095, 1705
AsuC2I CCSGG 1 cut(s) 1952
AsuHPI GGTGA 5 cut(s) 49, 208, 1306, 1424, 1696
AvaII GGWCC 2 cut(s) 554, 1095
BanII GRGCYC 3 cut(s) 867, 1326, 1423
BarI GAAGNNNNNNTAC 2 cut(s) 1322, 1354
Bbv12I GWGCWC 4 cut(s) 496, 867, 1326, 1552
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 8 cut(s) 181, 379, 401, 711, 1357, 1413, 1702, 1831
BccI CCATC 3 cut(s) 303, 1379, 1696
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 3 cut(s) 1055, 1211, 1739
BciVI GTATCC 1 cut(s) 1192
BcnI CCSGG 1 cut(s) 1952
BcoDI GTCTC 2 cut(s) 92, 521
BfaI CTAG 3 cut(s) 449, 837, 1635
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 843
BfrI CTTAAG 2 cut(s) 82, 1907
BfuI GTATCC 1 cut(s) 1192
BglII AGATCT 1 cut(s) 259
BisI GCNGC 9 cut(s) 195, 304, 390, 393, 725, 1346, 1427, 1691, 1845
BlpI GCTNAGC 2 cut(s) 866, 2010
BlsI GCNGC 9 cut(s) 196, 305, 391, 394, 726, 1347, 1428, 1692, 1846
BmcAI AGTACT 1 cut(s) 1331
Bme1390I CCNGG 4 cut(s) 1055, 1211, 1739, 1952
Bme18I GGWCC 2 cut(s) 554, 1095
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 4 cut(s) 305, 554, 1095, 1705
BmiI GGNNCC 5 cut(s) 438, 1045, 1097, 1537, 1707
BmrFI CCNGG 4 cut(s) 1055, 1211, 1739, 1952
BmrI ACTGGG 2 cut(s) 1426, 1703
BmsI GCATC 6 cut(s) 125, 646, 1151, 1677, 1702, 1873
BmuI ACTGGG 2 cut(s) 1426, 1703
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu1102I GCTNAGC 2 cut(s) 866, 2010
BpuEI CTTGAG 2 cut(s) 353, 1310
BpuMI CCSGG 1 cut(s) 1952
BsaBI GATNNNNATC 1 cut(s) 1180
BsaJI CCNNGG 3 cut(s) 894, 966, 1934
Bsc4I CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1714, 1715, 1957, 2024
Bse118I RCCGGY 2 cut(s) 544, 1244
Bse1I ACTGG 3 cut(s) 1097, 1421, 1709
Bse3DI GCAATG 3 cut(s) 1101, 1503, 1550
Bse8I GATNNNNATC 1 cut(s) 1180
BseBI CCWGG 3 cut(s) 1055, 1211, 1739
BseDI CCNNGG 3 cut(s) 894, 966, 1934
BseGI GGATG 5 cut(s) 661, 1219, 1298, 1390, 1693
BseJI GATNNNNATC 1 cut(s) 1180
BseLI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1714, 1715, 1957, 2024
BseMI GCAATG 3 cut(s) 1101, 1503, 1550
BseMII CTCAG 6 cut(s) 794, 796, 880, 1317, 1340, 2024
BseNI ACTGG 3 cut(s) 1097, 1421, 1709
BseXI GCAGC 8 cut(s) 181, 379, 401, 711, 1357, 1413, 1702, 1831
BsgI GTGCAG 1 cut(s) 1770
BshFI GGCC 5 cut(s) 306, 551, 643, 1706, 1757
BsiHKAI GWGCWC 4 cut(s) 496, 867, 1326, 1552
BsiSI CCGG 4 cut(s) 545, 552, 1245, 1951
BslFI GGGAC 2 cut(s) 65, 593
BslI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1714, 1715, 1957, 2024
BsmAI GTCTC 2 cut(s) 92, 521
BsmFI GGGAC 2 cut(s) 65, 593
BsmI GAATGC 2 cut(s) 269, 1777
BsnI GGCC 5 cut(s) 306, 551, 643, 1706, 1757
Bsp1286I GDGCHC 5 cut(s) 496, 867, 1326, 1423, 1552
Bsp1407I TGTACA 1 cut(s) 2002
Bsp143I GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
Bsp1720I GCTNAGC 2 cut(s) 866, 2010
Bsp19I CCATGG 1 cut(s) 1934
BspACI CCGC 4 cut(s) 67, 303, 934, 1481
BspANI GGCC 5 cut(s) 306, 551, 643, 1706, 1757
BspCNI CTCAG 6 cut(s) 795, 795, 879, 1316, 1341, 2023
BspHI TCATGA 1 cut(s) 1927
BspLI GGNNCC 5 cut(s) 438, 1045, 1097, 1537, 1707
BspPI GGATC 3 cut(s) 236, 662, 1183
BspQI GCTCTTC 1 cut(s) 1314
BspTI CTTAAG 2 cut(s) 82, 1907
BsrDI GCAATG 3 cut(s) 1101, 1503, 1550
BsrFI RCCGGY 2 cut(s) 544, 1244
BsrGI TGTACA 1 cut(s) 2002
BsrI ACTGG 3 cut(s) 1097, 1421, 1709
BssAI RCCGGY 2 cut(s) 544, 1244
BssECI CCNNGG 3 cut(s) 894, 966, 1934
BssMI GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
BssT1I CCWWGG 3 cut(s) 894, 966, 1934
Bst2UI CCWGG 3 cut(s) 1055, 1211, 1739
Bst4CI ACNGT 4 cut(s) 445, 532, 574, 682
Bst6I CTCTTC 1 cut(s) 1314
BstAFI CTTAAG 2 cut(s) 82, 1907
BstAPI GCANNNNNTGC 1 cut(s) 724
BstAUI TGTACA 1 cut(s) 2002
BstC8I GCNNGC 3 cut(s) 180, 1775, 1896
BstDEI CTNAG 8 cut(s) 782, 803, 866, 937, 1005, 1303, 1349, 2010
BstDSI CCRYGG 1 cut(s) 1934
BstF5I GGATG 5 cut(s) 661, 1219, 1298, 1390, 1693
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 8 cut(s) 244, 262, 670, 1021, 1178, 1792, 1841, 1927
BstMAI GTCTC 2 cut(s) 92, 521
BstMBI GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
BstMWI GCNNNNNNNGC 4 cut(s) 200, 275, 724, 942
BstNI CCWGG 3 cut(s) 1055, 1211, 1739
BstNSI RCATGY 4 cut(s) 718, 1401, 1890, 2009
BstSCI CCNGG 4 cut(s) 1053, 1209, 1737, 1950
BstSFI CTRYAG 1 cut(s) 573
BstV1I GCAGC 8 cut(s) 181, 379, 401, 711, 1357, 1413, 1702, 1831
BstX2I RGATCY 2 cut(s) 259, 667
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 259, 667
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 5 cut(s) 306, 551, 643, 1706, 1757
BtgI CCRYGG 1 cut(s) 1934
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 5 cut(s) 661, 1219, 1298, 1390, 1693
Cac8I GCNNGC 3 cut(s) 180, 1775, 1896
CciI TCATGA 1 cut(s) 1927
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 2 cut(s) 544, 1244
Cfr13I GGNCC 4 cut(s) 305, 554, 1095, 1705
CseI GACGC 2 cut(s) 899, 1208
Csp6I GTAC 4 cut(s) 859, 925, 1330, 2003
CspCI CAANNNNNGTGG 3 cut(s) 528, 563, 2103
CviAII CATG 9 cut(s) 645, 715, 874, 1398, 1523, 1887, 1928, 1935, 2006
CviQI GTAC 4 cut(s) 859, 925, 1330, 2003
DdeI CTNAG 8 cut(s) 782, 803, 866, 937, 1005, 1303, 1349, 2010
DpnI GATC 8 cut(s) 243, 261, 669, 1020, 1177, 1791, 1840, 1926
DpnII GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
DraI TTTAAA 2 cut(s) 708, 1437
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 1314
EarI CTCTTC 1 cut(s) 1314
Ecl136II GAGCTC 2 cut(s) 865, 1324
Eco130I CCWWGG 3 cut(s) 894, 966, 1934
Eco24I GRGCYC 3 cut(s) 867, 1326, 1423
Eco47I GGWCC 2 cut(s) 554, 1095
Eco47III AGCGCT 1 cut(s) 841
Eco53kI GAGCTC 2 cut(s) 865, 1324
Eco57I CTGAAG 1 cut(s) 1324
EcoICRI GAGCTC 2 cut(s) 865, 1324
EcoRI GAATTC 1 cut(s) 1266
EcoRII CCWGG 3 cut(s) 1053, 1209, 1737
EcoT14I CCWWGG 3 cut(s) 894, 966, 1934
EcoT22I ATGCAT 1 cut(s) 1888
EcoT38I GRGCYC 3 cut(s) 867, 1326, 1423
ErhI CCWWGG 3 cut(s) 894, 966, 1934
FaeI CATG 9 cut(s) 648, 718, 877, 1401, 1526, 1890, 1931, 1938, 2009
FalI AAGNNNNNCTT 2 cut(s) 1751, 1783
FaqI GGGAC 2 cut(s) 65, 593
FatI CATG 9 cut(s) 644, 714, 873, 1397, 1522, 1886, 1927, 1934, 2005
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 1 cut(s) 1975
FblI GTMKAC 2 cut(s) 1290, 1993
Fnu4HI GCNGC 9 cut(s) 195, 304, 390, 393, 725, 1346, 1427, 1691, 1845
FokI GGATG 5 cut(s) 668, 1226, 1285, 1397, 1680
FriOI GRGCYC 3 cut(s) 867, 1326, 1423
Fsp4HI GCNGC 9 cut(s) 195, 304, 390, 393, 725, 1346, 1427, 1691, 1845
FspBI CTAG 3 cut(s) 449, 837, 1635
GlaI GCGC 1 cut(s) 841
GluI GCNGC 9 cut(s) 195, 304, 390, 393, 725, 1346, 1427, 1691, 1845
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 5 cut(s) 306, 551, 643, 1706, 1757
HapII CCGG 4 cut(s) 545, 552, 1245, 1951
HgaI GACGC 2 cut(s) 899, 1208
HhaI GCGC 1 cut(s) 842
Hin1II CATG 9 cut(s) 648, 718, 877, 1401, 1526, 1890, 1931, 1938, 2009
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 8 cut(s) 61, 156, 701, 762, 805, 998, 1764, 2062
HpaII CCGG 4 cut(s) 545, 552, 1245, 1951
HphI GGTGA 5 cut(s) 49, 208, 1306, 1424, 1696
Hpy166II GTNNAC 7 cut(s) 232, 678, 927, 1291, 1403, 1565, 1994
Hpy188I TCNGA 9 cut(s) 126, 246, 441, 1090, 1306, 1365, 1446, 1663, 1838
Hpy188III TCNNGA 5 cut(s) 332, 809, 1173, 1223, 1928
Hpy8I GTNNAC 7 cut(s) 232, 678, 927, 1291, 1403, 1565, 1994
Hpy99I CGWCG 2 cut(s) 445, 915
HpyAV CCTTC 5 cut(s) 241, 788, 1117, 1158, 1374
HpyCH4III ACNGT 4 cut(s) 445, 532, 574, 682
HpyCH4IV ACGT 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 275, 724, 942
HpyF3I CTNAG 8 cut(s) 782, 803, 866, 937, 1005, 1303, 1349, 2010
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 9 cut(s) 648, 718, 877, 1401, 1526, 1890, 1931, 1938, 2009
HspAI GCGC 1 cut(s) 840
Kzo9I GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 5 cut(s) 16, 791, 1043, 1128, 1458
Lsp1109I GCAGC 8 cut(s) 181, 379, 401, 711, 1357, 1413, 1702, 1831
LweI GCATC 6 cut(s) 125, 646, 1151, 1677, 1702, 1873
MaeI CTAG 3 cut(s) 449, 837, 1635
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 4 cut(s) 1312, 1412, 1592, 1628
MalI GATC 8 cut(s) 243, 261, 669, 1020, 1177, 1791, 1840, 1926
MboI GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 2 cut(s) 259, 667
MhlI GDGCHC 5 cut(s) 496, 867, 1326, 1423, 1552
MlyI GAGTC 4 cut(s) 150, 756, 814, 1007
MmeI TCCRAC 4 cut(s) 346, 442, 464, 1896
Mph1103I ATGCAT 1 cut(s) 1888
MroXI GAANNNNTTC 1 cut(s) 1488
MseI TTAA 7 cut(s) 83, 707, 822, 1355, 1436, 1908, 1986
MslI CAYNNNNRTG 2 cut(s) 590, 1557
MspA1I CMGCKG 2 cut(s) 392, 1348
MspCI CTTAAG 2 cut(s) 82, 1907
MspI CCGG 4 cut(s) 545, 552, 1245, 1951
MspR9I CCNGG 4 cut(s) 1055, 1211, 1739, 1952
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 269, 1777
MvaI CCWGG 3 cut(s) 1055, 1211, 1739
MwoI GCNNNNNNNGC 4 cut(s) 200, 275, 724, 942
NciI CCSGG 1 cut(s) 1952
NcoI CCATGG 1 cut(s) 1934
NdeI CATATG 1 cut(s) 1975
NdeII GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
NlaIII CATG 9 cut(s) 648, 718, 877, 1401, 1526, 1890, 1931, 1938, 2009
NlaIV GGNNCC 5 cut(s) 438, 1045, 1097, 1537, 1707
NmuCI GTSAC 3 cut(s) 1312, 1412, 1592
NsiI ATGCAT 1 cut(s) 1888
NspI RCATGY 4 cut(s) 718, 1401, 1890, 2009
PagI TCATGA 1 cut(s) 1927
PciSI GCTCTTC 1 cut(s) 1314
PctI GAATGC 2 cut(s) 269, 1777
PdmI GAANNNNTTC 1 cut(s) 1488
PfeI GAWTC 4 cut(s) 61, 701, 1764, 2062
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 1053, 1737
PkrI GCNGC 9 cut(s) 196, 305, 391, 394, 726, 1347, 1428, 1692, 1846
PleI GAGTC 4 cut(s) 150, 756, 813, 1006
PpsI GAGTC 4 cut(s) 150, 756, 813, 1006
Psp124BI GAGCTC 2 cut(s) 867, 1326
Psp6I CCWGG 3 cut(s) 1053, 1209, 1737
PspGI CCWGG 3 cut(s) 1053, 1209, 1737
PspN4I GGNNCC 5 cut(s) 438, 1045, 1097, 1537, 1707
PspPI GGNCC 4 cut(s) 305, 554, 1095, 1705
PsuI RGATCY 2 cut(s) 259, 667
PvuII CAGCTG 2 cut(s) 392, 1348
RsaI GTAC 4 cut(s) 860, 926, 1331, 2004
RsaNI GTAC 4 cut(s) 859, 925, 1330, 2003
RseI CAYNNNNRTG 2 cut(s) 590, 1557
SacI GAGCTC 2 cut(s) 867, 1326
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 7 cut(s) 83, 707, 822, 1355, 1436, 1908, 1986
SatI GCNGC 9 cut(s) 195, 304, 390, 393, 725, 1346, 1427, 1691, 1845
Sau3AI GATC 8 cut(s) 241, 259, 667, 1018, 1175, 1789, 1838, 1924
Sau96I GGNCC 4 cut(s) 305, 554, 1095, 1705
ScaI AGTACT 1 cut(s) 1331
SchI GAGTC 4 cut(s) 150, 756, 814, 1007
ScrFI CCNGG 4 cut(s) 1055, 1211, 1739, 1952
SduI GDGCHC 5 cut(s) 496, 867, 1326, 1423, 1552
SfaNI GCATC 6 cut(s) 125, 646, 1151, 1677, 1702, 1873
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 2 cut(s) 554, 1095
SmiMI CAYNNNNRTG 2 cut(s) 590, 1557
SmlI CTYRAG 4 cut(s) 82, 332, 1325, 1907
SmoI CTYRAG 4 cut(s) 82, 332, 1325, 1907
SsiI CCGC 4 cut(s) 67, 303, 934, 1481
SspMI CTAG 3 cut(s) 449, 837, 1635
SstI GAGCTC 2 cut(s) 867, 1326
StyD4I CCNGG 4 cut(s) 1053, 1209, 1737, 1950
StyI CCWWGG 3 cut(s) 894, 966, 1934
TaaI ACNGT 4 cut(s) 445, 532, 574, 682
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 808
TaqII GACCGA 2 cut(s) 810, 2011
TatI WGTACW 2 cut(s) 1329, 2002
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 4 cut(s) 61, 701, 1764, 2062
Tru1I TTAA 7 cut(s) 83, 707, 822, 1355, 1436, 1908, 1986
Tru9I TTAA 7 cut(s) 83, 707, 822, 1355, 1436, 1908, 1986
TseFI GTSAC 3 cut(s) 1312, 1412, 1592
TseI GCWGC 8 cut(s) 194, 389, 392, 724, 1345, 1426, 1690, 1844
Tsp45I GTSAC 3 cut(s) 1312, 1412, 1592
TspDTI ATGAA 7 cut(s) 410, 579, 862, 1169, 1287, 1614, 2075
Van91I CCANNNNNTGG 1 cut(s) 650
Vha464I CTTAAG 2 cut(s) 82, 1907
VpaK11BI GGWCC 2 cut(s) 554, 1095
XapI RAATTY 4 cut(s) 1266, 1432, 1812, 1832
XceI RCATGY 4 cut(s) 718, 1401, 1890, 2009
XcmI CCANNNNNNNNNTGG 3 cut(s) 901, 964, 1529
XmiI GTMKAC 2 cut(s) 1290, 1993
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 3 cut(s) 449, 837, 1635
ZrmI AGTACT 1 cut(s) 1331
Zsp2I ATGCAT 1 cut(s) 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.