Rmu_sc0000288.1_g000035

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000288.1
OG0016402
Physical Location & Seq
Forward (+)
114654 .. 116795
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000288.1_g000035.1.cds

Sequence Viewer

Length: 2142 bp
atggataaaattagggatataccatttttctccaaagaggtgaagaagatatgggacgtgtggaatctgcagggctgtattctcttaagtctctcactgcaagtttctctgctcttggcatcatttcggaaaaaatgcagaagcaaattgctacgaggactcatctggtctgcgtatttgctcgccgattggattgctgctgttgcaatagggataatcaccaaatcccaagttgacacttgcgacccgaagggcaacaaagatcttttggcattctgggcttcctttcttctactccatcttggcggccctgataccattacctcttttgctcttgaggacaacgagttctgggttaggcacttggttggactaattgttcaagttcttgcagctgctgcttgtttctacctgacacttcataacaagttcttggttccgacagtactagtctttgttgttggaacaatcaagtttggggagagaacttgtgctctgtatcttgctagtctagaccattttggggagactgtattgccaaagccggtggctggacctgactatgaagaaactgtagcaatccatacttcaatgaggtcagtcgcagtctctacgacacaagaagtagtggtaccaatcaggccatatattggggatgatgcacataaagatccaaagtttacgattaagggaaatacaaaagaatctttaaacgacatgcaattgctgcaagaagcatattacctctttgagagcttcaaaagactcattgctggctttcttcttagctccaaagaccgagaactgagtcgagatttcttccttaaaagaacctatactagcgcttttcagttgatagagtacgagctcagcttcatgtatgaggttcttcacaccaaggttattgtggtgcgtcgcaaaattgggtacaccctccgcttagtatgcttctgtgcaaccattgttgccttggtgttcttcttttcggttgaaaagaaagagtttgtcaagtttgacattgatcttacttatgctttgtttagtggagccattttcctggacatcatatctgtctttatgcttatcttctctgactggaccctcattgccttcaacaacactaacatttggagcagatttattgctgctgtaataatgaaaagaggaaggtggtctggatcggtatcccaatacaatatgataaagaattgcctggatgagcgtccagaatgtgtctttactcttgccggttgtctgggtgttgggggaattctgggcagaaccaaaattgtctacttttcatcctcagaagcagtcaccgaagagctcaagtactttatcttcaggcagctgaggttaaaatctctgaaagcaaacaacagaagggatggaatggaggcatgttcacaaagaggtgactgggctctgctgctgaatttaaactatcagaaactcaaatggagcattggggattatcaatatgcggaaagtgttcttctctggcacattgcaacagaactctactatgctgaaagtggaaccgagcaatgtcctgattgtcgtcaaggtagacaagcaacacaaagggaatgtgatagtgacaatggactaaaactttgcaatttttgtgaaagtaacgccaggcgtagtatttgcaaacttgtttcagattatctgttttatcttctggtgatgcagcatccaatgatggccccagttttggggaattggcatatagtgttccaggacacttgtgcagaggccaagagattctttcgcaagcattcagtaaaagatcataaggaagcctgtaaaaagatttctggtgtcgaagccaaattcagatcggctgctgtgaagggaagtaaaagtaaatctgtgctgtttgatgcatgtatgcttgcagaacagcttaaggagttggaggcagatcatgacccatggaaactaatgagccgggtgtgggtggaattattgtcatatgcttccattaaatgtagaccgattgtacatgctcagcaaccaagtaggggtggagaactgatgactttcacttggctactgatgaatcatctgggcttgggaacacaattctacgagctggaaaaccaagctggaacaaaaatggtggcagacaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

713

Amino Acids

81.37

Weight (kDa)

8.73

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 631
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 3 cut(s) 1293, 1570, 1999
AciI CCGC 3 cut(s) 306, 937, 1484
AclWI GGATC 2 cut(s) 665, 1186
AcsI RAATTY 3 cut(s) 1269, 1435, 1838
AcuI CTGAAG 1 cut(s) 1327
AfaI GTAC 6 cut(s) 447, 633, 863, 929, 1334, 2010
AfeI AGCGCT 1 cut(s) 844
AfiI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1720, 1721, 1963, 2030
AflII CTTAAG 2 cut(s) 85, 1913
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 5 cut(s) 383, 591, 760, 992, 1114
AhlI ACTAGT 1 cut(s) 448
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 4 cut(s) 1056, 1212, 1640, 1743
AloI GAACNNNNNNTCC 4 cut(s) 332, 363, 364, 395
Alw21I GWGCWC 3 cut(s) 496, 870, 1329
Alw26I GTCTC 3 cut(s) 95, 521, 613
AlwI GGATC 2 cut(s) 665, 1186
AlwNI CAGNNNCTG 1 cut(s) 398
Aor51HI AGCGCT 1 cut(s) 844
AoxI GGCC 4 cut(s) 307, 641, 1710, 1761
ApoI RAATTY 3 cut(s) 1269, 1435, 1838
ArsI GACNNNNNNTTYG 2 cut(s) 1599, 1631
Asp700I GAANNNNTTC 1 cut(s) 1491
Asp718I GGTACC 1 cut(s) 631
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 4 cut(s) 308, 554, 1098, 1711
AsuC2I CCSGG 1 cut(s) 1958
AsuHPI GGTGA 5 cut(s) 52, 211, 1309, 1427, 1702
AvaII GGWCC 2 cut(s) 554, 1098
BanI GGYRCC 1 cut(s) 631
BanII GRGCYC 3 cut(s) 870, 1329, 1426
BarI GAAGNNNNNNTAC 4 cut(s) 615, 647, 1325, 1357
Bbv12I GWGCWC 3 cut(s) 496, 870, 1329
BbvCI CCTCAGC 1 cut(s) 1352
BccI CCATC 3 cut(s) 306, 1382, 1702
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 4 cut(s) 1058, 1214, 1642, 1745
BciVI GTATCC 1 cut(s) 1195
BcnI CCSGG 1 cut(s) 1958
BcoDI GTCTC 3 cut(s) 95, 521, 613
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 4 cut(s) 449, 507, 512, 840
BfmI CTRYAG 2 cut(s) 68, 573
BfoI RGCGCY 1 cut(s) 846
BfrI CTTAAG 2 cut(s) 85, 1913
BfuI GTATCC 1 cut(s) 1195
BglII AGATCT 1 cut(s) 262
BlpI GCTNAGC 2 cut(s) 869, 2016
BmcAI AGTACT 2 cut(s) 447, 1334
Bme1390I CCNGG 5 cut(s) 1058, 1214, 1642, 1745, 1958
Bme18I GGWCC 2 cut(s) 554, 1098
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 4 cut(s) 308, 554, 1098, 1711
BmiI GGNNCC 6 cut(s) 438, 633, 1048, 1100, 1540, 1713
BmrFI CCNGG 5 cut(s) 1058, 1214, 1642, 1745, 1958
BmrI ACTGGG 2 cut(s) 1429, 1709
BmsI GCATC 5 cut(s) 128, 649, 1683, 1708, 1879
BmuI ACTGGG 2 cut(s) 1429, 1709
Bpu10I CCTNAGC 1 cut(s) 1352
Bpu1102I GCTNAGC 2 cut(s) 869, 2016
BpuEI CTTGAG 2 cut(s) 356, 1313
BpuMI CCSGG 1 cut(s) 1958
BsaBI GATNNNNATC 1 cut(s) 1183
BsaJI CCNNGG 3 cut(s) 897, 969, 1940
Bsc4I CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1720, 1721, 1963, 2030
Bse118I RCCGGY 2 cut(s) 544, 1247
Bse1I ACTGG 3 cut(s) 1100, 1424, 1715
Bse3DI GCAATG 4 cut(s) 768, 1104, 1506, 1553
Bse8I GATNNNNATC 1 cut(s) 1183
BseBI CCWGG 4 cut(s) 1058, 1214, 1642, 1745
BseDI CCNNGG 3 cut(s) 897, 969, 1940
BseGI GGATG 5 cut(s) 661, 1222, 1301, 1393, 1699
BseJI GATNNNNATC 1 cut(s) 1183
BseLI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1720, 1721, 1963, 2030
BseMI GCAATG 4 cut(s) 768, 1104, 1506, 1553
BseMII CTCAG 5 cut(s) 797, 883, 1320, 1343, 2030
BseNI ACTGG 3 cut(s) 1100, 1424, 1715
BsgI GTGCAG 1 cut(s) 1776
BshFI GGCC 4 cut(s) 309, 643, 1712, 1763
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 3 cut(s) 496, 870, 1329
BsiSI CCGG 3 cut(s) 545, 1248, 1957
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1720, 1721, 1963, 2030
BsmAI GTCTC 3 cut(s) 95, 521, 613
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 2 cut(s) 272, 1783
BsnI GGCC 4 cut(s) 309, 643, 1712, 1763
Bsp1286I GDGCHC 4 cut(s) 496, 870, 1329, 1426
Bsp1407I TGTACA 1 cut(s) 2008
Bsp143I GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
Bsp1720I GCTNAGC 2 cut(s) 869, 2016
Bsp19I CCATGG 1 cut(s) 1940
BspACI CCGC 3 cut(s) 306, 937, 1484
BspANI GGCC 4 cut(s) 309, 643, 1712, 1763
BspCNI CTCAG 5 cut(s) 798, 882, 1319, 1344, 2029
BspHI TCATGA 1 cut(s) 1933
BspLI GGNNCC 6 cut(s) 438, 633, 1048, 1100, 1540, 1713
BspMAI CTGCAG 1 cut(s) 72
BspPI GGATC 2 cut(s) 665, 1186
BspQI GCTCTTC 1 cut(s) 1317
BspT107I GGYRCC 1 cut(s) 631
BspTI CTTAAG 2 cut(s) 85, 1913
BsrDI GCAATG 4 cut(s) 768, 1104, 1506, 1553
BsrFI RCCGGY 2 cut(s) 544, 1247
BsrGI TGTACA 1 cut(s) 2008
BsrI ACTGG 3 cut(s) 1100, 1424, 1715
BssAI RCCGGY 2 cut(s) 544, 1247
BssECI CCNNGG 3 cut(s) 897, 969, 1940
BssMI GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
BssT1I CCWWGG 3 cut(s) 897, 969, 1940
Bst2UI CCWGG 4 cut(s) 1058, 1214, 1642, 1745
Bst4CI ACNGT 3 cut(s) 445, 532, 574
Bst6I CTCTTC 1 cut(s) 1317
BstAFI CTTAAG 2 cut(s) 85, 1913
BstAPI GCANNNNNTGC 2 cut(s) 398, 727
BstAUI TGTACA 1 cut(s) 2008
BstC8I GCNNGC 4 cut(s) 183, 775, 1781, 1902
BstDEI CTNAG 7 cut(s) 785, 806, 869, 940, 1306, 1352, 2016
BstDSI CCRYGG 1 cut(s) 1940
BstF5I GGATG 5 cut(s) 661, 1222, 1301, 1393, 1699
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 7 cut(s) 265, 673, 1024, 1181, 1798, 1847, 1933
BstMAI GTCTC 3 cut(s) 95, 521, 613
BstMBI GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
BstMWI GCNNNNNNNGC 5 cut(s) 203, 278, 398, 727, 945
BstNI CCWGG 4 cut(s) 1058, 1214, 1642, 1745
BstNSI RCATGY 4 cut(s) 721, 1404, 1896, 2015
BstSCI CCNGG 5 cut(s) 1056, 1212, 1640, 1743, 1956
BstSFI CTRYAG 2 cut(s) 68, 573
BstX2I RGATCY 2 cut(s) 262, 670
BstXI CCANNNNNNTGG 1 cut(s) 1057
BstYI RGATCY 2 cut(s) 262, 670
BsuI GTATCC 1 cut(s) 1195
BsuRI GGCC 4 cut(s) 309, 643, 1712, 1763
BtgI CCRYGG 1 cut(s) 1940
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 5 cut(s) 661, 1222, 1301, 1393, 1699
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 4 cut(s) 183, 775, 1781, 1902
CaiI CAGNNNCTG 1 cut(s) 398
CciI TCATGA 1 cut(s) 1933
CfoI GCGC 1 cut(s) 845
Cfr10I RCCGGY 2 cut(s) 544, 1247
Cfr13I GGNCC 4 cut(s) 308, 554, 1098, 1711
CseI GACGC 2 cut(s) 902, 1211
Csp6I GTAC 6 cut(s) 446, 632, 862, 928, 1333, 2009
CspCI CAANNNNNGTGG 3 cut(s) 528, 563, 2109
CviAII CATG 7 cut(s) 718, 877, 1401, 1893, 1934, 1941, 2012
CviQI GTAC 6 cut(s) 446, 632, 862, 928, 1333, 2009
DdeI CTNAG 7 cut(s) 785, 806, 869, 940, 1306, 1352, 2016
DpnI GATC 7 cut(s) 264, 672, 1023, 1180, 1797, 1846, 1932
DpnII GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
DraI TTTAAA 2 cut(s) 711, 1440
Eam1104I CTCTTC 1 cut(s) 1317
EarI CTCTTC 1 cut(s) 1317
Ecl136II GAGCTC 2 cut(s) 868, 1327
Eco130I CCWWGG 3 cut(s) 897, 969, 1940
Eco24I GRGCYC 3 cut(s) 870, 1329, 1426
Eco47I GGWCC 2 cut(s) 554, 1098
Eco47III AGCGCT 1 cut(s) 844
Eco53kI GAGCTC 2 cut(s) 868, 1327
Eco57I CTGAAG 1 cut(s) 1327
EcoICRI GAGCTC 2 cut(s) 868, 1327
EcoRI GAATTC 1 cut(s) 1269
EcoRII CCWGG 4 cut(s) 1056, 1212, 1640, 1743
EcoT14I CCWWGG 3 cut(s) 897, 969, 1940
EcoT22I ATGCAT 1 cut(s) 1894
EcoT38I GRGCYC 3 cut(s) 870, 1329, 1426
ErhI CCWWGG 3 cut(s) 897, 969, 1940
FaeI CATG 7 cut(s) 721, 880, 1404, 1896, 1937, 1944, 2015
FalI AAGNNNNNCTT 2 cut(s) 1757, 1789
FaqI GGGAC 1 cut(s) 68
FatI CATG 7 cut(s) 717, 876, 1400, 1892, 1933, 1940, 2011
FauNDI CATATG 1 cut(s) 1981
FblI GTMKAC 3 cut(s) 1293, 1570, 1999
FokI GGATG 5 cut(s) 668, 1229, 1288, 1400, 1686
FriOI GRGCYC 3 cut(s) 870, 1329, 1426
FspBI CTAG 4 cut(s) 449, 507, 512, 840
GlaI GCGC 1 cut(s) 844
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 4 cut(s) 309, 643, 1712, 1763
HapII CCGG 3 cut(s) 545, 1248, 1957
HgaI GACGC 2 cut(s) 902, 1211
HhaI GCGC 1 cut(s) 845
Hin1II CATG 7 cut(s) 721, 880, 1404, 1896, 1937, 1944, 2015
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 7 cut(s) 64, 159, 704, 765, 808, 1770, 2068
HpaII CCGG 3 cut(s) 545, 1248, 1957
HphI GGTGA 5 cut(s) 52, 211, 1309, 1427, 1702
Hpy166II GTNNAC 7 cut(s) 235, 681, 930, 1294, 1406, 1571, 2000
Hpy188I TCNGA 8 cut(s) 129, 441, 1093, 1309, 1368, 1449, 1669, 1844
Hpy188III TCNNGA 7 cut(s) 335, 512, 812, 1176, 1226, 1553, 1934
Hpy8I GTNNAC 7 cut(s) 235, 681, 930, 1294, 1406, 1571, 2000
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 5 cut(s) 244, 1120, 1161, 1377, 1852
HpyCH4III ACNGT 3 cut(s) 445, 532, 574
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 278, 398, 727, 945
HpyF3I CTNAG 7 cut(s) 785, 806, 869, 940, 1306, 1352, 2016
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 7 cut(s) 721, 880, 1404, 1896, 1937, 1944, 2015
HspAI GCGC 1 cut(s) 843
KpnI GGTACC 1 cut(s) 635
Kzo9I GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 4 cut(s) 794, 1046, 1131, 1461
LweI GCATC 5 cut(s) 128, 649, 1683, 1708, 1879
MaeI CTAG 4 cut(s) 449, 507, 512, 840
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 4 cut(s) 1315, 1415, 1598, 1634
MalI GATC 7 cut(s) 264, 672, 1023, 1180, 1797, 1846, 1932
MboI GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
MfeI CAATTG 1 cut(s) 722
MflI RGATCY 2 cut(s) 262, 670
MhlI GDGCHC 4 cut(s) 496, 870, 1329, 1426
MlyI GAGTC 3 cut(s) 153, 759, 817
MmeI TCCRAC 4 cut(s) 349, 442, 464, 1902
Mph1103I ATGCAT 1 cut(s) 1894
MroXI GAANNNNTTC 1 cut(s) 1491
MseI TTAA 8 cut(s) 86, 687, 710, 825, 1358, 1439, 1914, 1992
MspA1I CMGCKG 2 cut(s) 395, 1351
MspCI CTTAAG 2 cut(s) 85, 1913
MspI CCGG 3 cut(s) 545, 1248, 1957
MspR9I CCNGG 5 cut(s) 1058, 1214, 1642, 1745, 1958
MunI CAATTG 1 cut(s) 722
Mva1269I GAATGC 2 cut(s) 272, 1783
MvaI CCWGG 4 cut(s) 1058, 1214, 1642, 1745
MwoI GCNNNNNNNGC 5 cut(s) 203, 278, 398, 727, 945
NciI CCSGG 1 cut(s) 1958
NcoI CCATGG 1 cut(s) 1940
NdeI CATATG 1 cut(s) 1981
NdeII GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
NlaIII CATG 7 cut(s) 721, 880, 1404, 1896, 1937, 1944, 2015
NlaIV GGNNCC 6 cut(s) 438, 633, 1048, 1100, 1540, 1713
NmuCI GTSAC 3 cut(s) 1315, 1415, 1598
NsiI ATGCAT 1 cut(s) 1894
NspI RCATGY 4 cut(s) 721, 1404, 1896, 2015
PagI TCATGA 1 cut(s) 1933
PciSI GCTCTTC 1 cut(s) 1317
PctI GAATGC 2 cut(s) 272, 1783
PdmI GAANNNNTTC 1 cut(s) 1491
PfeI GAWTC 4 cut(s) 64, 704, 1770, 2068
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 1056, 1743
PleI GAGTC 3 cut(s) 153, 759, 816
PpsI GAGTC 3 cut(s) 153, 759, 816
Psp124BI GAGCTC 2 cut(s) 870, 1329
Psp6I CCWGG 4 cut(s) 1056, 1212, 1640, 1743
PspGI CCWGG 4 cut(s) 1056, 1212, 1640, 1743
PspN4I GGNNCC 6 cut(s) 438, 633, 1048, 1100, 1540, 1713
PspPI GGNCC 4 cut(s) 308, 554, 1098, 1711
PstI CTGCAG 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 2 cut(s) 262, 670
PvuII CAGCTG 2 cut(s) 395, 1351
RsaI GTAC 6 cut(s) 447, 633, 863, 929, 1334, 2010
RsaNI GTAC 6 cut(s) 446, 632, 862, 928, 1333, 2009
SacI GAGCTC 2 cut(s) 870, 1329
SapI GCTCTTC 1 cut(s) 1317
SaqAI TTAA 8 cut(s) 86, 687, 710, 825, 1358, 1439, 1914, 1992
Sau3AI GATC 7 cut(s) 262, 670, 1021, 1178, 1795, 1844, 1930
Sau96I GGNCC 4 cut(s) 308, 554, 1098, 1711
ScaI AGTACT 2 cut(s) 447, 1334
SchI GAGTC 3 cut(s) 153, 759, 817
ScrFI CCNGG 5 cut(s) 1058, 1214, 1642, 1745, 1958
SduI GDGCHC 4 cut(s) 496, 870, 1329, 1426
SfaNI GCATC 5 cut(s) 128, 649, 1683, 1708, 1879
SfcI CTRYAG 2 cut(s) 68, 573
SinI GGWCC 2 cut(s) 554, 1098
SmlI CTYRAG 4 cut(s) 85, 335, 1328, 1913
SmoI CTYRAG 4 cut(s) 85, 335, 1328, 1913
SpeI ACTAGT 1 cut(s) 448
SsiI CCGC 3 cut(s) 306, 937, 1484
SspMI CTAG 4 cut(s) 449, 507, 512, 840
SstI GAGCTC 2 cut(s) 870, 1329
StyD4I CCNGG 5 cut(s) 1056, 1212, 1640, 1743, 1956
StyI CCWWGG 3 cut(s) 897, 969, 1940
TaaI ACNGT 3 cut(s) 445, 532, 574
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 811, 1830
TaqII GACCGA 2 cut(s) 813, 2017
TatI WGTACW 3 cut(s) 445, 1332, 2008
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 4 cut(s) 64, 704, 1770, 2068
Tru1I TTAA 8 cut(s) 86, 687, 710, 825, 1358, 1439, 1914, 1992
Tru9I TTAA 8 cut(s) 86, 687, 710, 825, 1358, 1439, 1914, 1992
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 3 cut(s) 1315, 1415, 1598
Tsp45I GTSAC 3 cut(s) 1315, 1415, 1598
TspDTI ATGAA 6 cut(s) 410, 579, 865, 1172, 1290, 2081
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 650
Vha464I CTTAAG 2 cut(s) 85, 1913
VpaK11BI GGWCC 2 cut(s) 554, 1098
XapI RAATTY 3 cut(s) 1269, 1435, 1838
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 4 cut(s) 721, 1404, 1896, 2015
XcmI CCANNNNNNNNNTGG 2 cut(s) 904, 967
XmiI GTMKAC 3 cut(s) 1293, 1570, 1999
XmnI GAANNNNTTC 1 cut(s) 1491
XspI CTAG 4 cut(s) 449, 507, 512, 840
ZrmI AGTACT 2 cut(s) 447, 1334
Zsp2I ATGCAT 1 cut(s) 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.