MD10G1323400.v1.1

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015567
Physical Location & Seq
Reverse (-)
40447678 .. 40451186
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1323400.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGCACACTGAAATTGTCGGAAGGAAAGCGGATGGGCGTTGGCTATGTGCCTCAATCGGATGTAGAGTGGTCACAAGCCGTGGGGTGGTAAAGCTCACAATCTTGAAGCCTCAAGGGTATTTAACTGGCATCAGTTATGGAGGAATGGACAATCTTTTAGATGTCAAGTCAAATGAAACCAGCAGAGGACTCAAAGGAGCTGAATATAGTGTTGTTCATCATAGCAATGACAGCTTAGAAGTTTCATTCAAGAACACCTTCATTCCATCATCCGCTAACGGCGTCAAATTGCCGCTAAGTGTGGATATAAGGTACATTGTAAAGACTGGTGTTTCAGGCTTCTACAATTACGCAATATATGAGCGGCCTTCTGGATGTCCTGCCTTCGATCTTGCGCAGACAAGGATGGCGTTTAAACTGCGAAGAGAGAAGTTCCACTACATGGCAATAACAGATGAGAAACAAAGGATAATGCCAATGCCTGAGGATGTGCTTCCACCAAGAGGCAAACAACTAATCGTGCCTGAATCAGTTTTGCTTTTAGATCCCATCAATCCTGATCTCAAAGGCGAGGTTGATGATAAGTATCAGTACTCGATGGACAACAAAGACAGTGGAGTCCATGGATGGATAAGTTCCGGCCCCACTGTAGGGTTTTGGCTCATCTTTCCCAGCCAGGAGTTCCGAAATGGCGGTCCTACGAAACAAAATCTTACCGTCCACACTGGTCCATTCTGCCTTGCTGTAAAAATGCTTCATGGAACTCATTATATCGGCGAAGATATATTAGCTCATTTCGAAGAAGGGGAGACTTGGACAAAAGTGTTTGGCCCCTTTTTCGTATACCTTAACTCAACCCCAGATGTATCAAAGGCACACGATTTATGGATTGATGCGAAAAAACAGAGGCTGATTGAAGAGACATTGTGGCCTTATGCTTTTGTTCAATCGCCCTACTATGTTGCCGCTAAAGAGCGTGGTTCAGTTTCAGGAAGATTGTTTGTCCAGGATAGGTATGTTTCGGATTCTCTCATTCCTGCTAAATACGCGTATGTTGGTCTATCAGTTGCAACTACTCCAGGATCCTGGCAAACAGAAAGCAAGGACTATCAGTTTTGGATACAAACAGATATCATCGGGAATTTCACCATCAAGAATGTTATCCCTGGAGTATACGGACTCCATGGCTGGATCCCGGGTTTTGTAGGTGATTACCTTGACAACGAACGTATCACAATCTCTGCAGATCTATCAATACAGGTAGCTAAACACATCAAATTGAAAACATGGCCTATTTTCCACTCAGGATCACAAACACAACTCGGAAACCTGACCTATGTTCCCCTCAGAGACGGTCCAACTTTATGGGAGATTGGCTACCCTGATAGGACGGCCATAGATTACTATGTTCCTGATGTGAACCCAATATACGTGAACAAGCTGTTTCTGAACAGCCCCGAGAAGTATAGACAGTATGGCTTGTGGGACAGATACACTGATGTGCACCCTGAATTTGACCAGACCTTCACTATAGGCAGCAGTAATCCGAAAACCGACTGGTTTTTTGCTCATGTGGACAGGAGGGGAGCAGATAATAAATACCTTCCAACAACATGGACAATAAAGTTCAATCTCAAATCTGTAACGACCGGCACATACAAGTTCAGACTGGCCATCGCTTCAGCAACCCGCTCGGACCTGAAGGTTCATGTTAATGCTATGGACATAGAACACCTGGTGATTCAAGTTTTGAACTTGGGGACGGATAACGCAGTTTGTCGACATGGAGTTCATGGACTCTATCGGCTTTTCAGCGGCGATATCCCATCAACGCTCCTGGTAAAGGGAGATAACTCCATATTTCTTTCACAGGCTAGGGGTGGTGATGCACTTTGTGGACTTTTCTATGATTATCTAAGACTGGAGGCTCCGGAAACAGCAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

650

Amino Acids

73.12

Weight (kDa)

6.84

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 22 - 63 1.2e-07 Rhamnogalacturonate lyase family
Rhamnogal_lyase PF06045 64 - 192 2.7e-45 Rhamnogalacturonate lyase family
fn3_3 PF14686 348 - 418 1.3e-17 Polysaccharide lyase family 4, domain II
CBM-like PF14683 454 - 641 8.3e-40 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015567)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01850
malus_domestica MD10G1323400.v1.1
prunus_persica Prupe.4G018900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002721
rosa_laevigata RLG00000031054
rosa_multiflora Rmu_sc0018891.1_g000001 Rmu_sc0018892.1_g000001 Rmu_sc0037618.1_g000002 Rmu_sc0041618.1_g000001
rosa_roxburghii Rroxscaffold_1G00073160
rosa_rugosa Rorug04G0398700
rosa_samantha Rh5AG021900 Rh5BG024800 Rh5CG024200 Rh5DG023200
rosa_wichuraiana Rw5G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
Acc16I TGCGCA 1 cut(s) 396
AccB7I CCANNNNNTGG 1 cut(s) 442
AccBSI CCGCTC 2 cut(s) 364, 1692
AccI GTMKAC 3 cut(s) 843, 1173, 1780
AccII CGCG 1 cut(s) 1049
AccIII TCCGGA 1 cut(s) 1930
AciI CCGC 8 cut(s) 29, 273, 293, 364, 693, 966, 1690, 1815
AclWI GGATC 6 cut(s) 539, 1077, 1090, 1186, 1199, 1315
AcoI YGGCCR 2 cut(s) 1392, 1671
AcsI RAATTY 2 cut(s) 1141, 1511
AcuI CTGAAG 2 cut(s) 1665, 1721
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 2 cut(s) 1738, 1895
AfaI GTAC 2 cut(s) 314, 593
AfiI CCNNNNNNNGG 6 cut(s) 85, 442, 503, 650, 651, 1843
AflIII ACRYGT 1 cut(s) 1047
AgsI TTSAA 8 cut(s) 106, 250, 917, 947, 1282, 1630, 1745, 1753
AjnI CCWGG 7 cut(s) 675, 1005, 1078, 1085, 1165, 1734, 1836
AleI CACNNNNGTG 1 cut(s) 1499
AluBI AGCT 7 cut(s) 94, 200, 234, 791, 1265, 1441, 1947
AluI AGCT 7 cut(s) 94, 200, 234, 791, 1265, 1441, 1947
Alw21I GWGCWC 1 cut(s) 1506
Alw26I GTCTC 3 cut(s) 803, 914, 1344
Alw44I GTGCAC 1 cut(s) 1502
AlwI GGATC 6 cut(s) 539, 1077, 1090, 1186, 1199, 1315
AlwNI CAGNNNCTG 1 cut(s) 910
Ama87I CYCGRG 2 cut(s) 1195, 1457
Aor13HI TCCGGA 1 cut(s) 1930
AoxI GGCC 7 cut(s) 365, 640, 829, 929, 1289, 1392, 1671
ApaLI GTGCAC 1 cut(s) 1502
ApeKI GCWGC 1 cut(s) 1536
ApoI RAATTY 2 cut(s) 1141, 1511
ArsI GACNNNNNNTTYG 4 cut(s) 679, 711, 1547, 1579
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 6 cut(s) 641, 695, 728, 830, 1355, 1696
AsuC2I CCSGG 2 cut(s) 1196, 1197
AsuHPI GGTGA 4 cut(s) 1138, 1220, 1750, 1895
AsuII TTCGAA 1 cut(s) 798
AvaI CYCGRG 2 cut(s) 1195, 1457
AvaII GGWCC 4 cut(s) 695, 728, 1355, 1696
AxyI CCTNAGG 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 1506
BaeI ACNNNNGTAYC 2 cut(s) 1213, 1246
BalI TGGCCA 1 cut(s) 1673
BamHI GGATCC 2 cut(s) 1082, 1191
BarI GAAGNNNNNNTAC 4 cut(s) 422, 454, 738, 770
Bbv12I GWGCWC 1 cut(s) 1506
BbvI GCAGC 1 cut(s) 1548
BccI CCATC 9 cut(s) 26, 274, 400, 557, 592, 621, 1157, 1682, 1834
BceAI ACGGC 3 cut(s) 63, 295, 1407
BcgI CGANNNNNNTGC 2 cut(s) 1674, 1708
BciT130I CCWGG 7 cut(s) 677, 1007, 1080, 1087, 1167, 1736, 1838
BciVI GTATCC 1 cut(s) 1113
BcnI CCSGG 2 cut(s) 1196, 1197
BcoDI GTCTC 3 cut(s) 803, 914, 1344
BfaI CTAG 1 cut(s) 1875
BfmI CTRYAG 3 cut(s) 648, 1242, 1530
BfuI GTATCC 1 cut(s) 1113
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 5 cut(s) 293, 365, 966, 1537, 1816
BlsI GCNGC 5 cut(s) 294, 366, 967, 1538, 1817
BmcAI AGTACT 1 cut(s) 593
Bme1390I CCNGG 9 cut(s) 677, 1007, 1080, 1087, 1167, 1196, 1197, 1736, 1838
Bme18I GGWCC 4 cut(s) 695, 728, 1355, 1696
BmeT110I CYCGRG 2 cut(s) 1195, 1457
BmgT120I GGNCC 6 cut(s) 641, 695, 728, 830, 1355, 1696
BmiI GGNNCC 5 cut(s) 643, 832, 1084, 1193, 1929
BmrFI CCNGG 9 cut(s) 677, 1007, 1080, 1087, 1167, 1196, 1197, 1736, 1838
BmsI GCATC 3 cut(s) 138, 883, 1876
BpmI CTGGAG 3 cut(s) 1062, 1188, 1943
Bpu14I TTCGAA 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 96
BpuMI CCSGG 2 cut(s) 1196, 1197
BsaAI YACGTR 1 cut(s) 1432
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 5 cut(s) 79, 622, 1165, 1183, 1195
BsaWI WCCGGW 1 cut(s) 1930
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 442, 503, 650, 651, 1843
Bse118I RCCGGY 1 cut(s) 1649
Bse1I ACTGG 6 cut(s) 130, 331, 730, 1562, 1674, 1926
Bse21I CCTNAGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 232
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 1930
BseBI CCWGG 7 cut(s) 677, 1007, 1080, 1087, 1167, 1736, 1838
BseDI CCNNGG 5 cut(s) 79, 622, 1165, 1183, 1195
BseGI GGATG 7 cut(s) 37, 65, 269, 380, 411, 493, 632
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 6 cut(s) 85, 442, 503, 650, 651, 1843
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 3 cut(s) 474, 1317, 1360
BseNI ACTGG 6 cut(s) 130, 331, 730, 1562, 1674, 1926
BseSI GKGCMC 1 cut(s) 1506
BseXI GCAGC 1 cut(s) 1548
BseYI CCCAGC 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 1049
Bsh1285I CGRYCG 1 cut(s) 1650
BshFI GGCC 7 cut(s) 367, 642, 831, 931, 1291, 1394, 1673
BsiEI CGRYCG 1 cut(s) 1650
BsiHKAI GWGCWC 1 cut(s) 1506
BsiHKCI CYCGRG 2 cut(s) 1195, 1457
BsiSI CCGG 4 cut(s) 639, 1196, 1650, 1931
BslFI GGGAC 2 cut(s) 1499, 1774
BslI CCNNNNNNNGG 6 cut(s) 85, 442, 503, 650, 651, 1843
BsmAI GTCTC 3 cut(s) 803, 914, 1344
BsmBI CGTCTC 1 cut(s) 1344
BsmFI GGGAC 2 cut(s) 1499, 1774
BsnI GGCC 7 cut(s) 367, 642, 831, 931, 1291, 1394, 1673
BsoBI CYCGRG 2 cut(s) 1195, 1457
Bsp119I TTCGAA 1 cut(s) 798
Bsp1286I GDGCHC 1 cut(s) 1506
Bsp13I TCCGGA 1 cut(s) 1930
Bsp143I GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
Bsp19I CCATGG 2 cut(s) 622, 1183
BspACI CCGC 8 cut(s) 29, 273, 293, 364, 693, 966, 1690, 1815
BspANI GGCC 7 cut(s) 367, 642, 831, 931, 1291, 1394, 1673
BspCNI CTCAG 3 cut(s) 475, 1316, 1359
BspEI TCCGGA 1 cut(s) 1930
BspFNI CGCG 1 cut(s) 1049
BspLI GGNNCC 5 cut(s) 643, 832, 1084, 1193, 1929
BspMAI CTGCAG 1 cut(s) 1246
BspPI GGATC 6 cut(s) 539, 1077, 1090, 1186, 1199, 1315
BspT104I TTCGAA 1 cut(s) 798
BsrBI CCGCTC 2 cut(s) 364, 1692
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 1649
BsrI ACTGG 6 cut(s) 130, 331, 730, 1562, 1674, 1926
BssAI RCCGGY 1 cut(s) 1649
BssECI CCNNGG 5 cut(s) 79, 622, 1165, 1183, 1195
BssMI GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
BssNAI GTATAC 2 cut(s) 844, 1174
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 2 cut(s) 622, 1183
Bst1107I GTATAC 2 cut(s) 844, 1174
Bst2UI CCWGG 7 cut(s) 677, 1007, 1080, 1087, 1167, 1736, 1838
Bst4CI ACNGT 5 cut(s) 614, 649, 718, 1355, 1473
Bst6I CTCTTC 2 cut(s) 418, 912
BstACI GRCGYC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 1432
BstBI TTCGAA 1 cut(s) 798
BstDEI CTNAG 6 cut(s) 235, 296, 483, 1303, 1346, 1916
BstDSI CCRYGG 3 cut(s) 79, 622, 1183
BstENI CCTNNNNNAGG 1 cut(s) 1841
BstF5I GGATG 7 cut(s) 37, 65, 269, 380, 411, 493, 632
BstFNI CGCG 1 cut(s) 1049
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 7 cut(s) 391, 547, 562, 1085, 1194, 1249, 1310
BstMAI GTCTC 3 cut(s) 803, 914, 1344
BstMBI GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
BstMCI CGRYCG 1 cut(s) 1650
BstMWI GCNNNNNNNGC 2 cut(s) 231, 1046
BstNI CCWGG 7 cut(s) 677, 1007, 1080, 1087, 1167, 1736, 1838
BstSCI CCNGG 9 cut(s) 675, 1005, 1078, 1085, 1165, 1194, 1195, 1734, 1836
BstSFI CTRYAG 3 cut(s) 648, 1242, 1530
BstSLI GKGCMC 1 cut(s) 1506
BstUI CGCG 1 cut(s) 1049
BstV1I GCAGC 1 cut(s) 1548
BstX2I RGATCY 4 cut(s) 544, 1082, 1191, 1246
BstXI CCANNNNNNTGG 3 cut(s) 1086, 1365, 1614
BstYI RGATCY 4 cut(s) 544, 1082, 1191, 1246
BstZ17I GTATAC 2 cut(s) 844, 1174
Bsu36I CCTNAGG 1 cut(s) 483
BsuI GTATCC 1 cut(s) 1113
BsuRI GGCC 7 cut(s) 367, 642, 831, 931, 1291, 1394, 1673
BtgI CCRYGG 3 cut(s) 79, 622, 1183
BtgZI GCGATG 1 cut(s) 1660
BtsCI GGATG 7 cut(s) 37, 65, 269, 380, 411, 493, 632
BtsIMutI CAGTG 5 cut(s) 6, 619, 645, 723, 1494
CaiI CAGNNNCTG 1 cut(s) 910
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 1649
Cfr13I GGNCC 6 cut(s) 641, 695, 728, 830, 1355, 1696
Cfr9I CCCGGG 1 cut(s) 1195
CseI GACGC 1 cut(s) 271
CsiI ACCWGGT 1 cut(s) 1734
Csp6I GTAC 2 cut(s) 313, 592
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviQI GTAC 2 cut(s) 313, 592
DdeI CTNAG 6 cut(s) 235, 296, 483, 1303, 1346, 1916
DpnI GATC 7 cut(s) 390, 546, 561, 1084, 1193, 1248, 1309
DpnII GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
DraI TTTAAA 1 cut(s) 415
DraIII CACNNNGTG 2 cut(s) 1738, 1895
DrdI GACNNNNNNGTC 1 cut(s) 617
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 2 cut(s) 1392, 1671
Eam1104I CTCTTC 2 cut(s) 418, 912
EarI CTCTTC 2 cut(s) 418, 912
Eco130I CCWWGG 2 cut(s) 622, 1183
Eco32I GATATC 2 cut(s) 1132, 1822
Eco47I GGWCC 4 cut(s) 695, 728, 1355, 1696
Eco57I CTGAAG 2 cut(s) 1665, 1721
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 2 cut(s) 1195, 1457
EcoNI CCTNNNNNAGG 1 cut(s) 1841
EcoRII CCWGG 7 cut(s) 675, 1005, 1078, 1085, 1165, 1734, 1836
EcoRV GATATC 2 cut(s) 1132, 1822
EcoT14I CCWWGG 2 cut(s) 622, 1183
ErhI CCWWGG 2 cut(s) 622, 1183
Esp3I CGTCTC 1 cut(s) 1344
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 2 cut(s) 1499, 1774
FauI CCCGC 1 cut(s) 1697
FblI GTMKAC 3 cut(s) 843, 1173, 1780
Fnu4HI GCNGC 5 cut(s) 293, 365, 966, 1537, 1816
FokI GGATG 7 cut(s) 44, 72, 256, 387, 418, 500, 639
Fsp4HI GCNGC 5 cut(s) 293, 365, 966, 1537, 1816
FspBI CTAG 1 cut(s) 1875
FspI TGCGCA 1 cut(s) 396
GlaI GCGC 1 cut(s) 396
GluI GCNGC 5 cut(s) 293, 365, 966, 1537, 1816
GsaI CCCAGC 1 cut(s) 675
GsuI CTGGAG 3 cut(s) 1062, 1188, 1943
HaeIII GGCC 7 cut(s) 367, 642, 831, 931, 1291, 1394, 1673
HapII CCGG 4 cut(s) 639, 1196, 1650, 1931
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 397
Hin1I GRCGYC 1 cut(s) 282
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 1781
HindII GTYRAC 1 cut(s) 1781
HinfI GANTC 7 cut(s) 189, 527, 618, 1025, 1179, 1741, 1797
HpaII CCGG 4 cut(s) 639, 1196, 1650, 1931
HphI GGTGA 4 cut(s) 1138, 1220, 1750, 1895
Hpy166II GTNNAC 9 cut(s) 721, 844, 1174, 1420, 1435, 1504, 1576, 1781, 1898
Hpy8I GTNNAC 9 cut(s) 721, 844, 1174, 1420, 1435, 1504, 1576, 1781, 1898
HpyAV CCTTC 8 cut(s) 15, 268, 378, 394, 797, 1534, 1613, 1696
HpyCH4III ACNGT 5 cut(s) 614, 649, 718, 1355, 1473
HpyCH4IV ACGT 2 cut(s) 1228, 1431
HpyCH4V TGCA 5 cut(s) 4, 1070, 1244, 1504, 1889
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 1046
HpyF3I CTNAG 6 cut(s) 235, 296, 483, 1303, 1346, 1916
HpySE526I ACGT 2 cut(s) 1228, 1431
Hsp92I GRCGYC 1 cut(s) 282
HspAI GCGC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 1930
Kzo9I GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
LmnI GCTCC 4 cut(s) 197, 1586, 1839, 1933
Lsp1109I GCAGC 1 cut(s) 1548
LweI GCATC 3 cut(s) 138, 883, 1876
MabI ACCWGGT 1 cut(s) 1734
MaeI CTAG 1 cut(s) 1875
MaeII ACGT 2 cut(s) 1228, 1431
MaeIII GTNAC 2 cut(s) 70, 1642
MalI GATC 7 cut(s) 390, 546, 561, 1084, 1193, 1248, 1309
MbiI CCGCTC 2 cut(s) 364, 1692
MboI GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
MboII GAAGA 5 cut(s) 435, 791, 812, 929, 1005
MflI RGATCY 4 cut(s) 544, 1082, 1191, 1246
MhlI GDGCHC 1 cut(s) 1506
MlsI TGGCCA 1 cut(s) 1673
MluCI AATT 6 cut(s) 12, 287, 346, 1141, 1277, 1511
MluI ACGCGT 1 cut(s) 1047
MluNI TGGCCA 1 cut(s) 1673
MlyI GAGTC 4 cut(s) 183, 627, 1173, 1791
MmeI TCCRAC 2 cut(s) 1382, 1631
Mox20I TGGCCA 1 cut(s) 1673
MroI TCCGGA 1 cut(s) 1930
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 1673
MseI TTAA 4 cut(s) 122, 414, 849, 1713
MslI CAYNNNNRTG 3 cut(s) 225, 1499, 1713
Msp20I TGGCCA 1 cut(s) 1673
MspA1I CMGCKG 1 cut(s) 1815
MspI CCGG 4 cut(s) 639, 1196, 1650, 1931
MspR9I CCNGG 9 cut(s) 677, 1007, 1080, 1087, 1167, 1196, 1197, 1736, 1838
MssI GTTTAAAC 1 cut(s) 415
MvaI CCWGG 7 cut(s) 677, 1007, 1080, 1087, 1167, 1736, 1838
MvnI CGCG 1 cut(s) 1049
MwoI GCNNNNNNNGC 2 cut(s) 231, 1046
NciI CCSGG 2 cut(s) 1196, 1197
NcoI CCATGG 2 cut(s) 622, 1183
NdeII GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
NlaIV GGNNCC 5 cut(s) 643, 832, 1084, 1193, 1929
NmuCI GTSAC 1 cut(s) 70
NsbI TGCGCA 1 cut(s) 396
NspV TTCGAA 1 cut(s) 798
OliI CACNNNNGTG 1 cut(s) 1499
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 3 cut(s) 527, 1025, 1741
PflMI CCANNNNNTGG 1 cut(s) 442
PfoI TCCNGGA 2 cut(s) 1005, 1078
PkrI GCNGC 5 cut(s) 294, 366, 967, 1538, 1817
PleI GAGTC 4 cut(s) 183, 626, 1173, 1791
PmeI GTTTAAAC 1 cut(s) 415
PpsI GAGTC 4 cut(s) 183, 626, 1173, 1791
Ppu21I YACGTR 1 cut(s) 1432
Psp6I CCWGG 7 cut(s) 675, 1005, 1078, 1085, 1165, 1734, 1836
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 7 cut(s) 675, 1005, 1078, 1085, 1165, 1734, 1836
PspN4I GGNNCC 5 cut(s) 643, 832, 1084, 1193, 1929
PspPI GGNCC 6 cut(s) 641, 695, 728, 830, 1355, 1696
PsrI GAACNNNNNNTAC 2 cut(s) 1412, 1444
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 1 cut(s) 910
PsuI RGATCY 4 cut(s) 544, 1082, 1191, 1246
RsaI GTAC 2 cut(s) 314, 593
RsaNI GTAC 2 cut(s) 313, 592
RseI CAYNNNNRTG 3 cut(s) 225, 1499, 1713
SalI GTCGAC 1 cut(s) 1779
SaqAI TTAA 4 cut(s) 122, 414, 849, 1713
SatI GCNGC 5 cut(s) 293, 365, 966, 1537, 1816
Sau3AI GATC 7 cut(s) 388, 544, 559, 1082, 1191, 1246, 1307
Sau96I GGNCC 6 cut(s) 641, 695, 728, 830, 1355, 1696
ScaI AGTACT 1 cut(s) 593
SchI GAGTC 4 cut(s) 183, 627, 1173, 1791
ScrFI CCNGG 9 cut(s) 677, 1007, 1080, 1087, 1167, 1196, 1197, 1736, 1838
SduI GDGCHC 1 cut(s) 1506
SexAI ACCWGGT 1 cut(s) 1734
SfaNI GCATC 3 cut(s) 138, 883, 1876
SfcI CTRYAG 3 cut(s) 648, 1242, 1530
SfuI TTCGAA 1 cut(s) 798
SinI GGWCC 4 cut(s) 695, 728, 1355, 1696
SmaI CCCGGG 1 cut(s) 1197
SmiMI CAYNNNNRTG 3 cut(s) 225, 1499, 1713
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
Sse9I AATT 6 cut(s) 12, 287, 346, 1141, 1277, 1511
SsiI CCGC 8 cut(s) 29, 273, 293, 364, 693, 966, 1690, 1815
SspMI CTAG 1 cut(s) 1875
StyD4I CCNGG 9 cut(s) 675, 1005, 1078, 1085, 1165, 1194, 1195, 1734, 1836
StyI CCWWGG 2 cut(s) 622, 1183
TaaI ACNGT 5 cut(s) 614, 649, 718, 1355, 1473
TaiI ACGT 2 cut(s) 1231, 1434
TaqI TCGA 4 cut(s) 387, 596, 798, 1780
TasI AATT 6 cut(s) 12, 287, 346, 1141, 1277, 1511
TatI WGTACW 1 cut(s) 591
TauI GCSGC 4 cut(s) 295, 367, 968, 1818
TfiI GAWTC 3 cut(s) 527, 1025, 1741
Tru1I TTAA 4 cut(s) 122, 414, 849, 1713
Tru9I TTAA 4 cut(s) 122, 414, 849, 1713
TscAI CASTG 5 cut(s) 13, 619, 652, 730, 1501
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 1536
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 7 cut(s) 189, 206, 234, 250, 746, 1697, 1781
TspGWI ACGGA 2 cut(s) 1191, 1778
TspMI CCCGGG 1 cut(s) 1195
TspRI CASTG 5 cut(s) 13, 619, 652, 730, 1501
Van91I CCANNNNNTGG 1 cut(s) 442
VneI GTGCAC 1 cut(s) 1502
VpaK11BI GGWCC 4 cut(s) 695, 728, 1355, 1696
XagI CCTNNNNNAGG 1 cut(s) 1841
XapI RAATTY 2 cut(s) 1141, 1511
XmaI CCCGGG 1 cut(s) 1195
XmiI GTMKAC 3 cut(s) 843, 1173, 1780
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 1875
ZrmI AGTACT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.