Rroxscaffold_1G00073160

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015567
Physical Location & Seq
Reverse (-)
94130650 .. 94134130
3481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073160.1

Sequence Viewer

Length: 2007 bp
ATGGCAAAGAAGAAAATGAAATTGAGCAGCCAAGGTCCTTATGTTACAATGGACAATGGGTTGGTGAAGCTTACAATTCTGAAGCCTCAAGGGTATATAATTGGCATTGCTTATGGAGGAATGGACAACCTTCTGGATGCTAAGTCCAATGAATCAAGTCGAGGATATTGGGACATCAACTGGAATTTGCCAGGAGGCCAAGACAGATACCATTTACTGGAAGGAGGTGAATATAGTGTTGTCCAAATGAACAAAGAAAGTTTAGAGGTTTCCTTCAAGAACACTTACGATCCATGGTCAGCTCGAAGCACAGATTTGCCTCTCACCGTAGACATAAGGTACATAGTAAGGACTGGTGTTTCAGGCTTCTACTGCTATGGAATTTATGAGCGGCCATCTGGTTGTCCGGCTTTCGATCTTGCTCAGACAAGACTAGTGTTTAAGCTTAGAAAAGACAAGTTCCACTACATGGCAATAACAGATGAGAAACAAAGGGTAATGCCTATGCCTGAGGATCTGCTTCCAGGTAGAGGCAAGCAACTAATTGTACCAGAGTCAGTTTTGCTAGTAAATCCTATAAATCCAGATCTCAAAGGGGAGGTTGACGATAAGTATATGTATTCGATGGACAACAAAGATAGTGGACTCCATGGATGGATAGGTTCTGGCCCCGTTATAGGATTTTGGACCATCTTTCCCAGCCAGGAGTTCAGAAATGGAGGACCTACAAAGCAAAACCTCACAGTTCACACTGGTCCAACCTGCCTTGCAATGTTTCAGGGAACACATTATCTTGGGTTAGATATGGTAGCTCAATTCGAAGAGGGAGAGACATGGAAGAAGGTGTTTGGACCCGTCTTTATATACCTTAACTCAACACCGGATATATCAAAAGCCCACAATTTATGGACAGATGCAAAGAAACAGAGGCTGTTTGAAGAGTCAGTGTGGCCTTATGATTTTGTCTCGTCACTCAACTATGTTGCTGCAAAAGACCGCGGTTCAGTTTCTGGAAAATTGTTAGTGCAGGACAGGTATATTTCGGGTTCTCTCATTCCTGCTAAAAATGCTTATATTGGTCTCTCGGTTGCAACTACTGCAGGATCATGGCAACTTGAAAGCAAGGGCTATCAGTTTTGGGTGCAAACAGATTCAAATGGAAACTTTACTATCAAGAATGTGATCCCTGGAGTATATGGACTTCATGGCTGGGTTCCTGGTTTTATGGGTGATTACCTTAACAGAGAACAGATCACTATCTCTGAAGGAAAACAAACAGAACTTGGAAATCTGACCTATGTTCCCCCGAGAGATGGTCCAACATTATGGGAGATTGGCTTGCCAGATCGAACAGCCATAGGTGACTTTGTTCCTGATGTGAACCCAATGTATGTCAACAAGTTGTTCATCAACAGCCCCGAAAAGTACAGACAATATGGCTTATGGGACAGATACACTGATATACACCCTCAACTTGACCAAATCTACACTATAGGAATCAGTAATCCCAAAACAGACTGGTTCTTTGCTCATGTGGACAGGAGAGGGCCAGGGAATAAGTATGTTCCAACAACATGGACAATCAAGTTCAATCTCAATTCAGTAACTACTGGTACTTACCAGCTGAGACTGTCCATTGCTTCAGCAACTCGCTCAGACCTTGAGGTCCATGTTAACTATATGGATACAGGGCACCTGGTGTTTCAAGAGTTGAACTTGGGCACAGACAACACACTTTGTCGACATGGAATTCATGGATTCTATTGCCTTTTCAGCGCCAACTTTCCCTCAACGTTGCTGGTGAAGGGAGATAATTCCATTTTTCTCACACAGGCGAGGGGTGGCGATTCACTTTGTGGAGTTCTCTATGATTATATAAGACTAGAAGCTCCTGCAACTTCAGAGAGCCTTAAAAGCTTATACCCCCTATTGAGTAGAAACTATAAAGTATTCAAATCATTTTATGGCAAACTAGTGTTTCGCAAAGATGTTCATAGTACTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

668

Amino Acids

75.38

Weight (kDa)

7.61

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 4 - 201 5.9e-69 Rhamnogalacturonate lyase family
fn3_3 PF14686 355 - 428 1.4e-22 Polysaccharide lyase family 4, domain II
CBM-like PF14683 441 - 628 3.9e-39 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015567)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01850
malus_domestica MD10G1323400.v1.1
prunus_persica Prupe.4G018900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002721
rosa_laevigata RLG00000031054
rosa_multiflora Rmu_sc0018891.1_g000001 Rmu_sc0018892.1_g000001 Rmu_sc0037618.1_g000002 Rmu_sc0041618.1_g000001
rosa_roxburghii Rroxscaffold_1G00073160
rosa_rugosa Rorug04G0398700
rosa_samantha Rh5AG021900 Rh5BG024800 Rh5CG024200 Rh5DG023200
rosa_wichuraiana Rw5G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1664
Acc36I ACCTGC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 1692
AccB7I CCANNNNNTGG 2 cut(s) 217, 469
AccBSI CCGCTC 1 cut(s) 391
AccI GTMKAC 2 cut(s) 330, 1741
AccII CGCG 1 cut(s) 999
AciI CCGC 3 cut(s) 391, 997, 999
AclI AACGTT 1 cut(s) 1793
AclWI GGATC 4 cut(s) 284, 522, 1111, 1177
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 3 cut(s) 184, 381, 1749
AcuI CTGAAG 4 cut(s) 101, 1284, 1626, 1884
AdeI CACNNNGTG 2 cut(s) 1699, 1856
AfaI GTAC 5 cut(s) 341, 549, 1427, 1615, 1999
AfiI CCNNNNNNNGG 5 cut(s) 217, 469, 530, 677, 1313
AgsI TTSAA 8 cut(s) 277, 938, 1118, 1155, 1591, 1706, 1714, 1954
AhlI ACTAGT 2 cut(s) 433, 1972
AjnI CCWGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
AluBI AGCT 7 cut(s) 70, 302, 445, 812, 1624, 1889, 1917
AluI AGCT 7 cut(s) 70, 302, 445, 812, 1624, 1889, 1917
Alw26I GTCTC 4 cut(s) 824, 970, 1085, 1621
AlwI GGATC 4 cut(s) 284, 522, 1111, 1177
AlwNI CAGNNNCTG 2 cut(s) 931, 1010
Ama87I CYCGRG 1 cut(s) 1306
AoxI GGCC 5 cut(s) 196, 392, 667, 950, 1547
ApeKI GCWGC 2 cut(s) 27, 986
ApoI RAATTY 3 cut(s) 184, 381, 1749
ArsI GACNNNNNNTTYG 2 cut(s) 1508, 1540
AspLEI GCGC 1 cut(s) 1778
AspS9I GGNCC 9 cut(s) 35, 668, 687, 722, 755, 851, 1316, 1547, 1666
AsuHPI GGTGA 6 cut(s) 76, 239, 316, 1241, 1373, 1813
AsuII TTCGAA 1 cut(s) 819
AvaI CYCGRG 1 cut(s) 1306
AvaII GGWCC 7 cut(s) 35, 687, 722, 755, 851, 1316, 1666
AxyI CCTNAGG 1 cut(s) 510
BaeGI GKGCMC 2 cut(s) 1695, 1724
BanI GGYRCC 1 cut(s) 1692
BarI GAAGNNNNNNTAC 2 cut(s) 1185, 1217
BbvI GCAGC 2 cut(s) 39, 973
BccI CCATC 5 cut(s) 403, 619, 648, 698, 1307
BciT130I CCWGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
BciVI GTATCC 1 cut(s) 1678
BcoDI GTCTC 4 cut(s) 824, 970, 1085, 1621
BcuI ACTAGT 2 cut(s) 433, 1972
BfaI CTAG 4 cut(s) 434, 566, 1883, 1973
BfmI CTRYAG 2 cut(s) 1098, 1491
BfoI RGCGCY 1 cut(s) 1779
BfuAI ACCTGC 1 cut(s) 770
BfuI GTATCC 1 cut(s) 1678
BglII AGATCT 1 cut(s) 586
BisI GCNGC 3 cut(s) 28, 392, 987
BlsI GCNGC 3 cut(s) 29, 393, 988
BmcAI AGTACT 1 cut(s) 1999
Bme1390I CCNGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
Bme18I GGWCC 7 cut(s) 35, 687, 722, 755, 851, 1316, 1666
BmeT110I CYCGRG 1 cut(s) 1306
BmgT120I GGNCC 9 cut(s) 35, 668, 687, 722, 755, 851, 1316, 1547, 1666
BmiI GGNNCC 4 cut(s) 670, 853, 1215, 1694
BmrFI CCNGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
BmsI GCATC 2 cut(s) 127, 904
BpmI CTGGAG 1 cut(s) 1209
Bpu14I TTCGAA 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 72, 1682
BsaBI GATNNNNATC 1 cut(s) 1256
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 6 cut(s) 31, 293, 649, 997, 1186, 1550
BsaWI WCCGGW 1 cut(s) 880
Bsc4I CCNNNNNNNGG 5 cut(s) 217, 469, 530, 677, 1313
Bse1I ACTGG 7 cut(s) 185, 222, 358, 757, 1523, 1615, 2005
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 3 cut(s) 105, 777, 1635
Bse8I GATNNNNATC 1 cut(s) 1256
BseBI CCWGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
BseDI CCNNGG 6 cut(s) 31, 293, 649, 997, 1186, 1550
BseGI GGATG 2 cut(s) 142, 659
BseJI GATNNNNATC 1 cut(s) 1256
BseLI CCNNNNNNNGG 5 cut(s) 217, 469, 530, 677, 1313
BseMI GCAATG 3 cut(s) 105, 777, 1635
BseMII CTCAG 4 cut(s) 437, 501, 1616, 1668
BseNI ACTGG 7 cut(s) 185, 222, 358, 757, 1523, 1615, 2005
BseSI GKGCMC 2 cut(s) 1695, 1724
BseXI GCAGC 2 cut(s) 39, 973
BseYI CCCAGC 2 cut(s) 698, 1209
BsgI GTGCAG 1 cut(s) 1046
Bsh1236I CGCG 1 cut(s) 999
BshFI GGCC 5 cut(s) 198, 394, 669, 952, 1549
BshNI GGYRCC 1 cut(s) 1692
BsiHKCI CYCGRG 1 cut(s) 1306
BsiSI CCGG 2 cut(s) 407, 881
BslFI GGGAC 2 cut(s) 185, 1460
BslI CCNNNNNNNGG 5 cut(s) 217, 469, 530, 677, 1313
BsmAI GTCTC 4 cut(s) 824, 970, 1085, 1621
BsmFI GGGAC 2 cut(s) 185, 1460
BsnI GGCC 5 cut(s) 198, 394, 669, 952, 1549
Bso31I GGTCTC 1 cut(s) 1085
BsoBI CYCGRG 1 cut(s) 1306
Bsp119I TTCGAA 1 cut(s) 819
Bsp1286I GDGCHC 2 cut(s) 1695, 1724
Bsp143I GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
Bsp19I CCATGG 2 cut(s) 293, 649
BspACI CCGC 3 cut(s) 391, 997, 999
BspANI GGCC 5 cut(s) 198, 394, 669, 952, 1549
BspCNI CTCAG 4 cut(s) 436, 502, 1617, 1667
BspFNI CGCG 1 cut(s) 999
BspLI GGNNCC 4 cut(s) 670, 853, 1215, 1694
BspMAI CTGCAG 1 cut(s) 1102
BspMI ACCTGC 1 cut(s) 770
BspPI GGATC 4 cut(s) 284, 522, 1111, 1177
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 1692
BspTNI GGTCTC 1 cut(s) 1085
BsrBI CCGCTC 1 cut(s) 391
BsrDI GCAATG 3 cut(s) 105, 777, 1635
BsrI ACTGG 7 cut(s) 185, 222, 358, 757, 1523, 1615, 2005
BssECI CCNNGG 6 cut(s) 31, 293, 649, 997, 1186, 1550
BssMI GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
BssT1I CCWWGG 3 cut(s) 31, 293, 649
Bst2UI CCWGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
Bst4CI ACNGT 3 cut(s) 328, 745, 1632
Bst6I CTCTTC 2 cut(s) 816, 933
BstAPI GCANNNNNTGC 1 cut(s) 1097
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 2 cut(s) 536, 1340
BstDEI CTNAG 6 cut(s) 141, 423, 446, 510, 1625, 1654
BstDSI CCRYGG 3 cut(s) 293, 649, 997
BstF5I GGATG 2 cut(s) 142, 659
BstFNI CGCG 1 cut(s) 999
BstH2I RGCGCY 1 cut(s) 1779
BstHHI GCGC 1 cut(s) 1778
BstKTI GATC 8 cut(s) 292, 418, 517, 589, 1106, 1185, 1254, 1348
BstMAI GTCTC 4 cut(s) 824, 970, 1085, 1621
BstMBI GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
BstMWI GCNNNNNNNGC 5 cut(s) 372, 1067, 1097, 1773, 1914
BstNI CCWGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
BstSCI CCNGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
BstSFI CTRYAG 2 cut(s) 1098, 1491
BstSLI GKGCMC 2 cut(s) 1695, 1724
BstUI CGCG 1 cut(s) 999
BstV1I GCAGC 2 cut(s) 39, 973
BstX2I RGATCY 2 cut(s) 514, 586
BstXI CCANNNNNNTGG 2 cut(s) 1326, 1575
BstYI RGATCY 2 cut(s) 514, 586
Bsu36I CCTNAGG 1 cut(s) 510
BsuI GTATCC 1 cut(s) 1678
BsuRI GGCC 5 cut(s) 198, 394, 669, 952, 1549
BtgI CCRYGG 3 cut(s) 293, 649, 997
BtsCI GGATG 2 cut(s) 142, 659
BtsIMutI CAGTG 3 cut(s) 750, 951, 1455
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 2 cut(s) 536, 1340
CaiI CAGNNNCTG 2 cut(s) 931, 1010
CfoI GCGC 1 cut(s) 1778
Cfr13I GGNCC 9 cut(s) 35, 668, 687, 722, 755, 851, 1316, 1547, 1666
Cfr42I CCGCGG 1 cut(s) 1000
CsiI ACCWGGT 1 cut(s) 1695
Csp6I GTAC 5 cut(s) 340, 548, 1426, 1614, 1998
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviQI GTAC 5 cut(s) 340, 548, 1426, 1614, 1998
DdeI CTNAG 6 cut(s) 141, 423, 446, 510, 1625, 1654
DpnI GATC 8 cut(s) 291, 417, 516, 588, 1105, 1184, 1253, 1347
DpnII GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
DraIII CACNNNGTG 2 cut(s) 1699, 1856
DrdI GACNNNNNNGTC 1 cut(s) 1664
DseDI GACNNNNNNGTC 1 cut(s) 1664
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 816, 933
EarI CTCTTC 2 cut(s) 816, 933
Eco130I CCWWGG 3 cut(s) 31, 293, 649
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 7 cut(s) 35, 687, 722, 755, 851, 1316, 1666
Eco57I CTGAAG 4 cut(s) 101, 1284, 1626, 1884
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 1306
EcoO109I RGGNCCY 2 cut(s) 35, 722
EcoRI GAATTC 1 cut(s) 1749
EcoRII CCWGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
EcoT14I CCWWGG 3 cut(s) 31, 293, 649
ErhI CCWWGG 3 cut(s) 31, 293, 649
FaqI GGGAC 2 cut(s) 185, 1460
FblI GTMKAC 2 cut(s) 330, 1741
Fnu4HI GCNGC 3 cut(s) 28, 392, 987
FokI GGATG 2 cut(s) 149, 666
Fsp4HI GCNGC 3 cut(s) 28, 392, 987
FspBI CTAG 4 cut(s) 434, 566, 1883, 1973
GlaI GCGC 1 cut(s) 1777
GluI GCNGC 3 cut(s) 28, 392, 987
GsaI CCCAGC 2 cut(s) 702, 1213
GsuI CTGGAG 1 cut(s) 1209
HaeII RGCGCY 1 cut(s) 1779
HaeIII GGCC 5 cut(s) 198, 394, 669, 952, 1549
HapII CCGG 2 cut(s) 407, 881
HhaI GCGC 1 cut(s) 1778
Hin6I GCGC 1 cut(s) 1776
HinP1I GCGC 1 cut(s) 1776
HincII GTYRAC 4 cut(s) 604, 1396, 1675, 1742
HindII GTYRAC 4 cut(s) 604, 1396, 1675, 1742
HindIII AAGCTT 3 cut(s) 68, 443, 1915
HinfI GANTC 8 cut(s) 152, 554, 645, 941, 1151, 1497, 1758, 1847
HpaI GTTAAC 1 cut(s) 1675
HpaII CCGG 2 cut(s) 407, 881
HphI GGTGA 6 cut(s) 76, 239, 316, 1241, 1373, 1813
Hpy166II GTNNAC 9 cut(s) 331, 604, 644, 748, 1381, 1396, 1537, 1675, 1742
Hpy188I TCNGA 7 cut(s) 81, 426, 713, 1264, 1293, 1657, 1903
Hpy188III TCNNGA 7 cut(s) 134, 277, 584, 1011, 1174, 1373, 1706
Hpy8I GTNNAC 9 cut(s) 331, 604, 644, 748, 1381, 1396, 1537, 1675, 1742
HpyAV CCTTC 6 cut(s) 140, 215, 283, 835, 1259, 1798
HpyCH4III ACNGT 3 cut(s) 328, 745, 1632
HpyCH4IV ACGT 1 cut(s) 1793
HpyCH4V TGCA 8 cut(s) 770, 917, 989, 1027, 1091, 1100, 1144, 1895
HpyF10VI GCNNNNNNNGC 5 cut(s) 372, 1067, 1097, 1773, 1914
HpyF3I CTNAG 6 cut(s) 141, 423, 446, 510, 1625, 1654
HpySE526I ACGT 1 cut(s) 1793
HspAI GCGC 1 cut(s) 1776
KspAI GTTAAC 1 cut(s) 1675
KspI CCGCGG 1 cut(s) 1000
Kzo9I GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
LmnI GCTCC 1 cut(s) 1894
Lsp1109I GCAGC 2 cut(s) 39, 973
LweI GCATC 2 cut(s) 127, 904
MabI ACCWGGT 1 cut(s) 1695
MaeI CTAG 4 cut(s) 434, 566, 1883, 1973
MaeII ACGT 1 cut(s) 1793
MaeIII GTNAC 4 cut(s) 43, 969, 1361, 1603
MalI GATC 8 cut(s) 291, 417, 516, 588, 1105, 1184, 1253, 1347
MbiI CCGCTC 1 cut(s) 391
MboI GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
MboII GAAGA 4 cut(s) 22, 833, 850, 950
MflI RGATCY 2 cut(s) 514, 586
MhlI GDGCHC 2 cut(s) 1695, 1724
MlyI GAGTC 3 cut(s) 563, 639, 950
MmeI TCCRAC 3 cut(s) 782, 1343, 1592
MseI TTAA 5 cut(s) 441, 870, 1239, 1674, 1911
MspA1I CMGCKG 2 cut(s) 999, 1624
MspI CCGG 2 cut(s) 407, 881
MspR9I CCNGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
MvaI CCWGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 5 cut(s) 372, 1067, 1097, 1773, 1914
NcoI CCATGG 2 cut(s) 293, 649
NdeII GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
NlaIV GGNNCC 4 cut(s) 670, 853, 1215, 1694
NmuCI GTSAC 2 cut(s) 969, 1361
NspV TTCGAA 1 cut(s) 819
PfeI GAWTC 5 cut(s) 152, 1151, 1497, 1758, 1847
PflMI CCANNNNNTGG 2 cut(s) 217, 469
PkrI GCNGC 3 cut(s) 29, 393, 988
PleI GAGTC 3 cut(s) 562, 639, 949
PpsI GAGTC 3 cut(s) 562, 639, 949
PpuMI RGGWCCY 2 cut(s) 35, 722
Psp1406I AACGTT 1 cut(s) 1793
Psp5II RGGWCCY 2 cut(s) 35, 722
Psp6I CCWGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
PspFI CCCAGC 2 cut(s) 698, 1209
PspGI CCWGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
PspN4I GGNNCC 4 cut(s) 670, 853, 1215, 1694
PspPI GGNCC 9 cut(s) 35, 668, 687, 722, 755, 851, 1316, 1547, 1666
PspPPI RGGWCCY 2 cut(s) 35, 722
PstI CTGCAG 1 cut(s) 1102
PstNI CAGNNNCTG 2 cut(s) 931, 1010
PsuI RGATCY 2 cut(s) 514, 586
PvuII CAGCTG 1 cut(s) 1624
RsaI GTAC 5 cut(s) 341, 549, 1427, 1615, 1999
RsaNI GTAC 5 cut(s) 340, 548, 1426, 1614, 1998
SacII CCGCGG 1 cut(s) 1000
SalI GTCGAC 1 cut(s) 1740
SaqAI TTAA 5 cut(s) 441, 870, 1239, 1674, 1911
SatI GCNGC 3 cut(s) 28, 392, 987
Sau3AI GATC 8 cut(s) 289, 415, 514, 586, 1103, 1182, 1251, 1345
Sau96I GGNCC 9 cut(s) 35, 668, 687, 722, 755, 851, 1316, 1547, 1666
ScaI AGTACT 1 cut(s) 1999
SchI GAGTC 3 cut(s) 563, 639, 950
ScrFI CCNGG 7 cut(s) 192, 525, 704, 1188, 1218, 1551, 1697
SduI GDGCHC 2 cut(s) 1695, 1724
SexAI ACCWGGT 1 cut(s) 1695
SfaNI GCATC 2 cut(s) 127, 904
SfcI CTRYAG 2 cut(s) 1098, 1491
Sfr303I CCGCGG 1 cut(s) 1000
SfuI TTCGAA 1 cut(s) 819
SgrBI CCGCGG 1 cut(s) 1000
SinI GGWCC 7 cut(s) 35, 687, 722, 755, 851, 1316, 1666
SmlI CTYRAG 2 cut(s) 87, 1661
SmoI CTYRAG 2 cut(s) 87, 1661
SpeI ACTAGT 2 cut(s) 433, 1972
SsiI CCGC 3 cut(s) 391, 997, 999
SspMI CTAG 4 cut(s) 434, 566, 1883, 1973
StyD4I CCNGG 7 cut(s) 190, 523, 702, 1186, 1216, 1549, 1695
StyI CCWWGG 3 cut(s) 31, 293, 649
TaaI ACNGT 3 cut(s) 328, 745, 1632
TaiI ACGT 1 cut(s) 1796
TaqI TCGA 7 cut(s) 160, 304, 414, 623, 819, 1348, 1741
TatI WGTACW 2 cut(s) 1425, 1997
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 5 cut(s) 152, 1151, 1497, 1758, 1847
Tru1I TTAA 5 cut(s) 441, 870, 1239, 1674, 1911
Tru9I TTAA 5 cut(s) 441, 870, 1239, 1674, 1911
TscAI CASTG 3 cut(s) 757, 951, 1462
TseFI GTSAC 2 cut(s) 969, 1361
TseI GCWGC 2 cut(s) 27, 986
Tsp45I GTSAC 2 cut(s) 969, 1361
TspDTI ATGAA 7 cut(s) 32, 165, 263, 1193, 1396, 1742, 1982
TspRI CASTG 3 cut(s) 757, 951, 1462
Van91I CCANNNNNTGG 2 cut(s) 217, 469
VpaK11BI GGWCC 7 cut(s) 35, 687, 722, 755, 851, 1316, 1666
XapI RAATTY 3 cut(s) 184, 381, 1749
XmiI GTMKAC 2 cut(s) 330, 1741
XspI CTAG 4 cut(s) 434, 566, 1883, 1973
ZrmI AGTACT 1 cut(s) 1999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.