Rw5G002040

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015567
Physical Location & Seq
Forward (+)
1757682 .. 1760946
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002040.1

Sequence Viewer

Length: 1890 bp
ATGGACAATGGGTTGGTGAAGCTTACAATTGTGAAGCCTCAAGGGTATATAATTGGCATTGCTTATGGAGGAATGGACAACCTTCTGGATGCCAAGTCCAATGAATCAAGTCGAGGATATTGGGACATCAACTGGAATTTGCCAGGAGGCCAAGACAGATACCATTTACTGGAAGGAGGTGAATATAGTGTTGTCCAAATGAACAAAGAAAGTTTAGAGGTTTCCTTCAAGAACACTTACGATCCATGGTCAGCTCGAAGCACAGATTTGCCTCTCACCGTGGACATAAGGTACATAGTAAGGACTGGTGTTTCAGGCTTCTACTGCTATGGAATTTATGAGCGGCCATCTGGTTGTCCTGCTTTCGATCTTGCTCAGACAAGACTGGTGTTTAAGCTTAGAAAAGACAAGTTCCACTACATGGCAATAACAGATGAGAAACAAAGGGTAATGCCTATGCCTGAGGATCTGCTTCCAGGTAGAGGCAAGCAACTAATTGTACCAGAGTCAGTTTTGCTAGTAAATCCCATAAATCCAGATCTCAAAGGGGAGGTTGACGATAAGTATATGTATTCAATGGATAACAAAGATAGTGGACTCCATGGATGGATAGGTTCTGGCCCCATTATCGGATTTTGGACCATCCTTCCGAGCCAGGAGTTCAGAAATGGAGGACCTACAAAGCAAAACCTCACAGTTCACACTGGTCCAACCTGCCTTGCAATGTTTCAGGGAACACATTATCTTGGGTTAGATATGGTAGCTCAATTCGAAGAGGGAGAGACATGGAAGAAGGTGTTTGGACCCGTCTTTATATACCTTAACTCAACACCAGATACATCAAAAGCCCACAATTTATGGACAGATGCAAAGAAACAGAGGCTGTTTGAAGAGACAGTGTGGCCTTATGATTTTGTCTCGTCACCCAACTATGTTGCTGCAAAAGACCGCGGTTCAGTTTCTGGAAGATTGTTAGTGCAGGACAGGTATATTTCGGGTTCTCTCATTCCTGCTAAAAATGCTTATATTGGTCTCTCGGTTGCAACTACTGCAGGATCATGGCAACTTGAAAGCAAGGGCTATCAGTTCTGGGTACAAACAGATTCAAATGGAAACTTTAATATCAAGAATGTGATCCCTGGAGTATATGGACTTCATGGCTGGGTTCCTGGTTTCATGGGTGATTACCTTCACAAAGAACAAATCACTATCTCTGAAGGAAAACAAACAGAACTTCAGAATCTGACCTATGTTCCCCCAAGAGATGGTCCAACATTATGGGAGATTGGCTTCCCTGATCGAACAGCCATAGGTGAATTTGTTCCGGATGTGAACCCAATGTATGTCAACAAGTTGTTCATCAACAGCCCCGAAACGTACAGACAATATGGCTTATGGGACAGATACACTGACATACACCCTCAACTTGACCAAATCTACACTATAGGAATCAGTAATCCCAAAACAGACTGGTTCTTTGCTCATGTGGACAGGAGAGGGCCAGGGAATAAGTATGTTCCAACAACATGGACAATCAAGTTCAATCTCAATTCAGTAACTACTGGCACTTACCAGCTGAGACTGTCCATAGCTTCAGCAACCCGCTCAGACCTTGAGGTCCATGTTAACTATATGGATTCAGGGCACCTGGTGTTTCAAGAGTTGAACTTGGGCACAGACAACAGTGTTTGTCGACATGGAATTCATGGATTCTATTGCCTTTTCAGTGCCAACTTTCCCTCAACGTTGCTGGTGAAGGGAGATAATTCCATTTTTCTCACACAGGCGAGGGGTGGCGATTCACTTTGTGGAGTTCTCTATGATTATATAAGACTAGAAGCTCCTGCAACTTCAGAGAGCCCTAAAAGCTTATACCCCCCTATTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

629

Amino Acids

70.89

Weight (kDa)

5.83

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 1 - 185 3.9e-67 Rhamnogalacturonate lyase family
fn3_3 PF14686 339 - 412 4.6e-22 Polysaccharide lyase family 4, domain II
CBM-like PF14683 425 - 612 2.3e-39 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015567)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01850
malus_domestica MD10G1323400.v1.1
prunus_persica Prupe.4G018900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002721
rosa_laevigata RLG00000031054
rosa_multiflora Rmu_sc0018891.1_g000001 Rmu_sc0018892.1_g000001 Rmu_sc0037618.1_g000002 Rmu_sc0041618.1_g000001
rosa_roxburghii Rroxscaffold_1G00073160
rosa_rugosa Rorug04G0398700
rosa_samantha Rh5AG021900 Rh5BG024800 Rh5CG024200 Rh5DG023200
rosa_wichuraiana Rw5G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1616
Acc36I ACCTGC 1 cut(s) 722
AccB1I GGYRCC 1 cut(s) 1644
AccB7I CCANNNNNTGG 3 cut(s) 169, 421, 1265
AccBSI CCGCTC 2 cut(s) 343, 1605
AccI GTMKAC 1 cut(s) 1693
AccII CGCG 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 1324
AciI CCGC 4 cut(s) 343, 949, 951, 1603
AclI AACGTT 1 cut(s) 1745
AclWI GGATC 4 cut(s) 236, 474, 1063, 1129
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 4 cut(s) 136, 333, 1316, 1701
AcuI CTGAAG 4 cut(s) 1220, 1236, 1578, 1836
AdeI CACNNNGTG 2 cut(s) 1651, 1808
AfaI GTAC 4 cut(s) 293, 501, 1095, 1379
AfiI CCNNNNNNNGG 5 cut(s) 169, 421, 482, 629, 1265
AgsI TTSAA 8 cut(s) 229, 576, 890, 1070, 1107, 1543, 1658, 1666
AjnI CCWGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
AluBI AGCT 8 cut(s) 22, 254, 397, 764, 1576, 1592, 1841, 1869
AluI AGCT 8 cut(s) 22, 254, 397, 764, 1576, 1592, 1841, 1869
Alw26I GTCTC 5 cut(s) 776, 887, 922, 1037, 1573
AlwI GGATC 4 cut(s) 236, 474, 1063, 1129
AlwNI CAGNNNCTG 3 cut(s) 883, 962, 1243
Aor13HI TCCGGA 1 cut(s) 1324
AoxI GGCC 5 cut(s) 148, 344, 619, 902, 1499
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 4 cut(s) 136, 333, 1316, 1701
ArsI GACNNNNNNTTYG 2 cut(s) 1460, 1492
Asp700I GAANNNNTTC 1 cut(s) 1320
AspS9I GGNCC 8 cut(s) 620, 639, 674, 707, 803, 1268, 1499, 1618
AsuHPI GGTGA 7 cut(s) 28, 191, 268, 915, 1193, 1325, 1765
AsuII TTCGAA 1 cut(s) 771
AvaII GGWCC 6 cut(s) 639, 674, 707, 803, 1268, 1618
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 2 cut(s) 1647, 1676
BanI GGYRCC 1 cut(s) 1644
BanII GRGCYC 1 cut(s) 1862
BarI GAAGNNNNNNTAC 2 cut(s) 1137, 1169
BbvI GCAGC 1 cut(s) 925
BccI CCATC 4 cut(s) 355, 600, 650, 1259
BciT130I CCWGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
BcoDI GTCTC 5 cut(s) 776, 887, 922, 1037, 1573
BfaI CTAG 2 cut(s) 518, 1835
BfmI CTRYAG 2 cut(s) 1050, 1443
BfuAI ACCTGC 1 cut(s) 722
BglII AGATCT 1 cut(s) 538
BisI GCNGC 2 cut(s) 344, 939
BlsI GCNGC 2 cut(s) 345, 940
Bme1390I CCNGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
Bme18I GGWCC 6 cut(s) 639, 674, 707, 803, 1268, 1618
BmgT120I GGNCC 8 cut(s) 620, 639, 674, 707, 803, 1268, 1499, 1618
BmiI GGNNCC 4 cut(s) 622, 805, 1167, 1646
BmrFI CCNGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
BmsI GCATC 2 cut(s) 79, 856
BpmI CTGGAG 1 cut(s) 1161
Bpu14I TTCGAA 1 cut(s) 771
BpuEI CTTGAG 2 cut(s) 24, 1634
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 6 cut(s) 245, 279, 601, 949, 1138, 1502
BsaWI WCCGGW 1 cut(s) 1324
Bsc4I CCNNNNNNNGG 5 cut(s) 169, 421, 482, 629, 1265
Bse1I ACTGG 7 cut(s) 137, 174, 310, 390, 709, 1475, 1567
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 2 cut(s) 57, 729
BseAI TCCGGA 1 cut(s) 1324
BseBI CCWGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
BseDI CCNNGG 6 cut(s) 245, 279, 601, 949, 1138, 1502
BseGI GGATG 4 cut(s) 94, 611, 642, 1333
BseLI CCNNNNNNNGG 5 cut(s) 169, 421, 482, 629, 1265
BseMI GCAATG 2 cut(s) 57, 729
BseMII CTCAG 4 cut(s) 389, 453, 1568, 1620
BseNI ACTGG 7 cut(s) 137, 174, 310, 390, 709, 1475, 1567
BseSI GKGCMC 2 cut(s) 1647, 1676
BseXI GCAGC 1 cut(s) 925
BseYI CCCAGC 1 cut(s) 1161
BsgI GTGCAG 1 cut(s) 998
Bsh1236I CGCG 1 cut(s) 951
BshFI GGCC 5 cut(s) 150, 346, 621, 904, 1501
BshNI GGYRCC 1 cut(s) 1644
BsiSI CCGG 1 cut(s) 1325
BslFI GGGAC 2 cut(s) 137, 1412
BslI CCNNNNNNNGG 5 cut(s) 169, 421, 482, 629, 1265
BsmAI GTCTC 5 cut(s) 776, 887, 922, 1037, 1573
BsmFI GGGAC 2 cut(s) 137, 1412
BsnI GGCC 5 cut(s) 150, 346, 621, 904, 1501
Bso31I GGTCTC 1 cut(s) 1037
Bsp119I TTCGAA 1 cut(s) 771
Bsp1286I GDGCHC 3 cut(s) 1647, 1676, 1862
Bsp13I TCCGGA 1 cut(s) 1324
Bsp143I GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
Bsp19I CCATGG 2 cut(s) 245, 601
BspACI CCGC 4 cut(s) 343, 949, 951, 1603
BspANI GGCC 5 cut(s) 150, 346, 621, 904, 1501
BspCNI CTCAG 4 cut(s) 388, 454, 1569, 1619
BspEI TCCGGA 1 cut(s) 1324
BspFNI CGCG 1 cut(s) 951
BspLI GGNNCC 4 cut(s) 622, 805, 1167, 1646
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 722
BspPI GGATC 4 cut(s) 236, 474, 1063, 1129
BspT104I TTCGAA 1 cut(s) 771
BspT107I GGYRCC 1 cut(s) 1644
BspTNI GGTCTC 1 cut(s) 1037
BsrBI CCGCTC 2 cut(s) 343, 1605
BsrDI GCAATG 2 cut(s) 57, 729
BsrI ACTGG 7 cut(s) 137, 174, 310, 390, 709, 1475, 1567
BssECI CCNNGG 6 cut(s) 245, 279, 601, 949, 1138, 1502
BssMI GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
BssT1I CCWWGG 2 cut(s) 245, 601
Bst2UI CCWGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
Bst4CI ACNGT 5 cut(s) 280, 697, 898, 1584, 1685
Bst6I CTCTTC 2 cut(s) 768, 885
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstBI TTCGAA 1 cut(s) 771
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 5 cut(s) 375, 398, 462, 1577, 1606
BstDSI CCRYGG 4 cut(s) 245, 279, 601, 949
BstF5I GGATG 4 cut(s) 94, 611, 642, 1333
BstFNI CGCG 1 cut(s) 951
BstKTI GATC 7 cut(s) 244, 370, 469, 541, 1058, 1137, 1300
BstMAI GTCTC 5 cut(s) 776, 887, 922, 1037, 1573
BstMBI GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
BstMWI GCNNNNNNNGC 4 cut(s) 324, 1019, 1049, 1866
BstNI CCWGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
BstSCI CCNGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
BstSFI CTRYAG 2 cut(s) 1050, 1443
BstSLI GKGCMC 2 cut(s) 1647, 1676
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 1 cut(s) 925
BstX2I RGATCY 2 cut(s) 466, 538
BstXI CCANNNNNNTGG 2 cut(s) 1278, 1527
BstYI RGATCY 2 cut(s) 466, 538
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 5 cut(s) 150, 346, 621, 904, 1501
BtgI CCRYGG 4 cut(s) 245, 279, 601, 949
BtsCI GGATG 4 cut(s) 94, 611, 642, 1333
BtsIMutI CAGTG 5 cut(s) 702, 903, 1407, 1690, 1732
BveI ACCTGC 1 cut(s) 722
Cac8I GCNNGC 1 cut(s) 488
CaiI CAGNNNCTG 3 cut(s) 883, 962, 1243
Cfr13I GGNCC 8 cut(s) 620, 639, 674, 707, 803, 1268, 1499, 1618
Cfr42I CCGCGG 1 cut(s) 952
CsiI ACCWGGT 1 cut(s) 1647
Csp6I GTAC 4 cut(s) 292, 500, 1094, 1378
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviQI GTAC 4 cut(s) 292, 500, 1094, 1378
DdeI CTNAG 5 cut(s) 375, 398, 462, 1577, 1606
DpnI GATC 7 cut(s) 243, 369, 468, 540, 1057, 1136, 1299
DpnII GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
DraIII CACNNNGTG 2 cut(s) 1651, 1808
DrdI GACNNNNNNGTC 1 cut(s) 1616
DseDI GACNNNNNNGTC 1 cut(s) 1616
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 768, 885
EarI CTCTTC 2 cut(s) 768, 885
Eco130I CCWWGG 2 cut(s) 245, 601
Eco24I GRGCYC 1 cut(s) 1862
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 6 cut(s) 639, 674, 707, 803, 1268, 1618
Eco57I CTGAAG 4 cut(s) 1220, 1236, 1578, 1836
Eco81I CCTNAGG 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 1701
EcoRII CCWGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
EcoT14I CCWWGG 2 cut(s) 245, 601
EcoT38I GRGCYC 1 cut(s) 1862
ErhI CCWWGG 2 cut(s) 245, 601
FaqI GGGAC 2 cut(s) 137, 1412
FauI CCCGC 1 cut(s) 1610
FblI GTMKAC 1 cut(s) 1693
Fnu4HI GCNGC 2 cut(s) 344, 939
FokI GGATG 4 cut(s) 101, 618, 629, 1340
FriOI GRGCYC 1 cut(s) 1862
Fsp4HI GCNGC 2 cut(s) 344, 939
FspBI CTAG 2 cut(s) 518, 1835
GluI GCNGC 2 cut(s) 344, 939
GsaI CCCAGC 1 cut(s) 1165
GsuI CTGGAG 1 cut(s) 1161
HaeIII GGCC 5 cut(s) 150, 346, 621, 904, 1501
HapII CCGG 1 cut(s) 1325
HincII GTYRAC 4 cut(s) 556, 1348, 1627, 1694
HindII GTYRAC 4 cut(s) 556, 1348, 1627, 1694
HindIII AAGCTT 3 cut(s) 20, 395, 1867
HinfI GANTC 9 cut(s) 104, 506, 597, 1103, 1240, 1449, 1637, 1710, 1799
HpaI GTTAAC 1 cut(s) 1627
HpaII CCGG 1 cut(s) 1325
HphI GGTGA 7 cut(s) 28, 191, 268, 915, 1193, 1325, 1765
Hpy166II GTNNAC 9 cut(s) 283, 556, 596, 700, 1333, 1348, 1489, 1627, 1694
Hpy188I TCNGA 9 cut(s) 378, 632, 651, 665, 1216, 1239, 1245, 1609, 1855
Hpy188III TCNNGA 7 cut(s) 86, 229, 536, 963, 1126, 1325, 1658
Hpy8I GTNNAC 9 cut(s) 283, 556, 596, 700, 1333, 1348, 1489, 1627, 1694
HpyAV CCTTC 8 cut(s) 92, 167, 235, 656, 787, 1199, 1211, 1750
HpyCH4III ACNGT 5 cut(s) 280, 697, 898, 1584, 1685
HpyCH4IV ACGT 2 cut(s) 1376, 1745
HpyCH4V TGCA 7 cut(s) 722, 869, 941, 979, 1043, 1052, 1847
HpyF10VI GCNNNNNNNGC 4 cut(s) 324, 1019, 1049, 1866
HpyF3I CTNAG 5 cut(s) 375, 398, 462, 1577, 1606
HpySE526I ACGT 2 cut(s) 1376, 1745
Kpn2I TCCGGA 1 cut(s) 1324
KspAI GTTAAC 1 cut(s) 1627
KspI CCGCGG 1 cut(s) 952
Kzo9I GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
LmnI GCTCC 1 cut(s) 1846
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 2 cut(s) 79, 856
MabI ACCWGGT 1 cut(s) 1647
MaeI CTAG 2 cut(s) 518, 1835
MaeII ACGT 2 cut(s) 1376, 1745
MaeIII GTNAC 2 cut(s) 921, 1555
MalI GATC 7 cut(s) 243, 369, 468, 540, 1057, 1136, 1299
MbiI CCGCTC 2 cut(s) 343, 1605
MboI GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
MboII GAAGA 4 cut(s) 785, 802, 902, 978
MfeI CAATTG 1 cut(s) 27
MflI RGATCY 2 cut(s) 466, 538
MhlI GDGCHC 3 cut(s) 1647, 1676, 1862
MlyI GAGTC 2 cut(s) 515, 591
MmeI TCCRAC 3 cut(s) 734, 1295, 1544
MroI TCCGGA 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 1320
MseI TTAA 4 cut(s) 393, 822, 1119, 1626
MspA1I CMGCKG 2 cut(s) 951, 1576
MspI CCGG 1 cut(s) 1325
MspR9I CCNGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
MunI CAATTG 1 cut(s) 27
MvaI CCWGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 4 cut(s) 324, 1019, 1049, 1866
NcoI CCATGG 2 cut(s) 245, 601
NdeII GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
NlaIV GGNNCC 4 cut(s) 622, 805, 1167, 1646
NmuCI GTSAC 1 cut(s) 921
NspV TTCGAA 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 1320
PfeI GAWTC 7 cut(s) 104, 1103, 1240, 1449, 1637, 1710, 1799
PflMI CCANNNNNTGG 3 cut(s) 169, 421, 1265
PkrI GCNGC 2 cut(s) 345, 940
PleI GAGTC 2 cut(s) 514, 591
PpsI GAGTC 2 cut(s) 514, 591
PpuMI RGGWCCY 1 cut(s) 674
Psp1406I AACGTT 1 cut(s) 1745
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
PspFI CCCAGC 1 cut(s) 1161
PspGI CCWGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
PspN4I GGNNCC 4 cut(s) 622, 805, 1167, 1646
PspPI GGNCC 8 cut(s) 620, 639, 674, 707, 803, 1268, 1499, 1618
PspPPI RGGWCCY 1 cut(s) 674
PstI CTGCAG 1 cut(s) 1054
PstNI CAGNNNCTG 3 cut(s) 883, 962, 1243
PsuI RGATCY 2 cut(s) 466, 538
PvuII CAGCTG 1 cut(s) 1576
RsaI GTAC 4 cut(s) 293, 501, 1095, 1379
RsaNI GTAC 4 cut(s) 292, 500, 1094, 1378
SacII CCGCGG 1 cut(s) 952
SalI GTCGAC 1 cut(s) 1692
SaqAI TTAA 4 cut(s) 393, 822, 1119, 1626
SatI GCNGC 2 cut(s) 344, 939
Sau3AI GATC 7 cut(s) 241, 367, 466, 538, 1055, 1134, 1297
Sau96I GGNCC 8 cut(s) 620, 639, 674, 707, 803, 1268, 1499, 1618
SchI GAGTC 2 cut(s) 515, 591
ScrFI CCNGG 7 cut(s) 144, 477, 656, 1140, 1170, 1503, 1649
SduI GDGCHC 3 cut(s) 1647, 1676, 1862
SexAI ACCWGGT 1 cut(s) 1647
SfaNI GCATC 2 cut(s) 79, 856
SfcI CTRYAG 2 cut(s) 1050, 1443
Sfr303I CCGCGG 1 cut(s) 952
SfuI TTCGAA 1 cut(s) 771
SgrBI CCGCGG 1 cut(s) 952
SinI GGWCC 6 cut(s) 639, 674, 707, 803, 1268, 1618
SmlI CTYRAG 2 cut(s) 39, 1613
SmoI CTYRAG 2 cut(s) 39, 1613
SsiI CCGC 4 cut(s) 343, 949, 951, 1603
SspMI CTAG 2 cut(s) 518, 1835
StyD4I CCNGG 7 cut(s) 142, 475, 654, 1138, 1168, 1501, 1647
StyI CCWWGG 2 cut(s) 245, 601
TaaI ACNGT 5 cut(s) 280, 697, 898, 1584, 1685
TaiI ACGT 2 cut(s) 1379, 1748
TaqI TCGA 6 cut(s) 112, 256, 366, 771, 1300, 1693
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 7 cut(s) 104, 1103, 1240, 1449, 1637, 1710, 1799
Tru1I TTAA 4 cut(s) 393, 822, 1119, 1626
Tru9I TTAA 4 cut(s) 393, 822, 1119, 1626
TscAI CASTG 5 cut(s) 709, 903, 1414, 1690, 1732
TseFI GTSAC 1 cut(s) 921
TseI GCWGC 1 cut(s) 938
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 6 cut(s) 117, 215, 1145, 1165, 1348, 1694
TspRI CASTG 5 cut(s) 709, 903, 1414, 1690, 1732
Van91I CCANNNNNTGG 3 cut(s) 169, 421, 1265
VpaK11BI GGWCC 6 cut(s) 639, 674, 707, 803, 1268, 1618
XapI RAATTY 4 cut(s) 136, 333, 1316, 1701
XmiI GTMKAC 1 cut(s) 1693
XmnI GAANNNNTTC 1 cut(s) 1320
XspI CTAG 2 cut(s) 518, 1835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.