Rmu_sc0037618.1_g000002

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0037618.1
OG0015567
Physical Location & Seq
Forward (+)
2460 .. 5009
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0037618.1_g000002.1.cds

Sequence Viewer

Length: 1401 bp
atggcaaagaagaaaatgaaattgagcagccaaggtccttatgttactatggacaatgggttggtgaagcttacaattgtgaagcctcaagggtatataattggcattgcttatggaggaatggacaaccttctggatgccaagtccaatgaatcaagtcgaggactggaaggaggtgaatatagtgtggtccaaatgaacaaagaaagtttagaggtttccttcaagaacatttacgatccatggtcagctcgaagcacagatttgcctctcaccgtggacataaggtacatagtaaggactggtgtttcaggcttctactgctatggaatttatgagcggccatctggttgtccggctttcgatcttgctcagacaagactggtgtttaagcttagaaaagacaagttccactacatggcaataacagatgagaaacaaagggtaatgcctatgcctgaggatctgcttccaggtagaggcaagcagctaattgtaccagagtcagttttgctagtaaatcccataaatccagatctcaaaggggaggttgacgataagtatatgtattcaatggataacaaagatagtggactccatggatggataggttctggccccattatcggattttggaccatccttccgagccaggagttcagaaatggaggacctacaaagcaaaacctcacagttcacactggtccaacctgccttgcaatgtttcagggaacacattatcttgggttagatatggtagctcaattcgaagagggagagacatggaagaaggtgtttggacccgtctttatataccttaactcaacaccagatacatcaaaagcccacaatttatggacagatgcaaagaaacagaggctgtttgaagagacagtgtggccttatgattttgtctcgtcacccaactatgttgctgcaaaagaccgcggttcagtttctggaagattgttagtgcaggacaggtatatttcgggttctctcattcctgctaaaaatgcttatattggtctctcggttgcaactactgcaggatcatggcaacttgaaagcaagggctatcagttctgggtacaaacagattcaaatggaaactttaatatcaagaatgtgatccctggagtatatggacttcatggctgggttcctggtttcatgggtgattaccttcaccaagaacaaatcactatctctgaaggtacttaccgcaagcttcaaatgataaccatacaaaacactatcacaaaaataaaggttcctaaactagcatcacttaaatgcatttgttttcaaaaatgcctcaggaaaacaaacagaacttcagaatctaacctatgttcccccaagagatggtccaacattatgggagattggcttccctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

466

Amino Acids

52.38

Weight (kDa)

8.95

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015567)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01850
malus_domestica MD10G1323400.v1.1
prunus_persica Prupe.4G018900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002721
rosa_laevigata RLG00000031054
rosa_multiflora Rmu_sc0018891.1_g000001 Rmu_sc0018892.1_g000001 Rmu_sc0037618.1_g000002 Rmu_sc0041618.1_g000001
rosa_roxburghii Rroxscaffold_1G00073160
rosa_rugosa Rorug04G0398700
rosa_samantha Rh5AG021900 Rh5BG024800 Rh5CG024200 Rh5DG023200
rosa_wichuraiana Rw5G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 719
AccB7I CCANNNNNTGG 2 cut(s) 418, 1368
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 1 cut(s) 948
AciI CCGC 4 cut(s) 340, 946, 948, 1225
AclWI GGATC 4 cut(s) 233, 471, 1060, 1126
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 1 cut(s) 330
AcuI CTGAAG 2 cut(s) 1233, 1323
AfaI GTAC 4 cut(s) 290, 498, 1092, 1219
AfiI CCNNNNNNNGG 4 cut(s) 418, 479, 626, 1368
AgsI TTSAA 7 cut(s) 226, 573, 887, 1067, 1104, 1235, 1310
AjnI CCWGG 4 cut(s) 472, 651, 1135, 1165
AluBI AGCT 6 cut(s) 70, 251, 394, 490, 761, 1231
AluI AGCT 6 cut(s) 70, 251, 394, 490, 761, 1231
Alw26I GTCTC 4 cut(s) 773, 884, 919, 1034
AlwI GGATC 4 cut(s) 233, 471, 1060, 1126
AlwNI CAGNNNCTG 2 cut(s) 880, 959
AoxI GGCC 3 cut(s) 341, 616, 899
ApeKI GCWGC 3 cut(s) 27, 487, 935
ApoI RAATTY 1 cut(s) 330
AspS9I GGNCC 8 cut(s) 35, 190, 617, 636, 671, 704, 800, 1371
AsuHPI GGTGA 6 cut(s) 76, 188, 265, 912, 1181, 1190
AsuII TTCGAA 1 cut(s) 768
AvaII GGWCC 7 cut(s) 35, 190, 636, 671, 704, 800, 1371
AxyI CCTNAGG 2 cut(s) 459, 1319
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BbvI GCAGC 3 cut(s) 39, 499, 922
BccI CCATC 4 cut(s) 352, 597, 647, 1362
BciT130I CCWGG 4 cut(s) 474, 653, 1137, 1167
BcoDI GTCTC 4 cut(s) 773, 884, 919, 1034
BfaI CTAG 2 cut(s) 515, 1283
BfmI CTRYAG 1 cut(s) 1047
BfuAI ACCTGC 1 cut(s) 719
BglII AGATCT 1 cut(s) 535
BisI GCNGC 4 cut(s) 28, 341, 488, 936
BlsI GCNGC 4 cut(s) 29, 342, 489, 937
Bme1390I CCNGG 4 cut(s) 474, 653, 1137, 1167
Bme18I GGWCC 7 cut(s) 35, 190, 636, 671, 704, 800, 1371
BmgT120I GGNCC 8 cut(s) 35, 190, 617, 636, 671, 704, 800, 1371
BmiI GGNNCC 4 cut(s) 619, 802, 1164, 1275
BmrFI CCNGG 4 cut(s) 474, 653, 1137, 1167
BmsI GCATC 3 cut(s) 127, 853, 1295
BpmI CTGGAG 1 cut(s) 1158
Bpu14I TTCGAA 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1034
BsaJI CCNNGG 6 cut(s) 31, 242, 276, 598, 946, 1135
Bsc4I CCNNNNNNNGG 4 cut(s) 418, 479, 626, 1368
Bse1I ACTGG 4 cut(s) 171, 307, 387, 706
Bse21I CCTNAGG 2 cut(s) 459, 1319
Bse3DI GCAATG 2 cut(s) 105, 726
BseBI CCWGG 4 cut(s) 474, 653, 1137, 1167
BseDI CCNNGG 6 cut(s) 31, 242, 276, 598, 946, 1135
BseGI GGATG 3 cut(s) 142, 608, 639
BseLI CCNNNNNNNGG 4 cut(s) 418, 479, 626, 1368
BseMI GCAATG 2 cut(s) 105, 726
BseMII CTCAG 3 cut(s) 386, 450, 1333
BseNI ACTGG 4 cut(s) 171, 307, 387, 706
BseXI GCAGC 3 cut(s) 39, 499, 922
BseYI CCCAGC 1 cut(s) 1158
BsgI GTGCAG 1 cut(s) 995
Bsh1236I CGCG 1 cut(s) 948
BshFI GGCC 3 cut(s) 343, 618, 901
BsiSI CCGG 1 cut(s) 356
BslI CCNNNNNNNGG 4 cut(s) 418, 479, 626, 1368
BsmAI GTCTC 4 cut(s) 773, 884, 919, 1034
BsnI GGCC 3 cut(s) 343, 618, 901
Bso31I GGTCTC 1 cut(s) 1034
Bsp119I TTCGAA 1 cut(s) 768
Bsp143I GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
Bsp19I CCATGG 2 cut(s) 242, 598
BspACI CCGC 4 cut(s) 340, 946, 948, 1225
BspANI GGCC 3 cut(s) 343, 618, 901
BspCNI CTCAG 3 cut(s) 385, 451, 1332
BspFNI CGCG 1 cut(s) 948
BspLI GGNNCC 4 cut(s) 619, 802, 1164, 1275
BspMAI CTGCAG 1 cut(s) 1051
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 4 cut(s) 233, 471, 1060, 1126
BspT104I TTCGAA 1 cut(s) 768
BspTNI GGTCTC 1 cut(s) 1034
BsrBI CCGCTC 1 cut(s) 340
BsrDI GCAATG 2 cut(s) 105, 726
BsrI ACTGG 4 cut(s) 171, 307, 387, 706
BssECI CCNNGG 6 cut(s) 31, 242, 276, 598, 946, 1135
BssMI GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
BssT1I CCWWGG 3 cut(s) 31, 242, 598
Bst2UI CCWGG 4 cut(s) 474, 653, 1137, 1167
Bst4CI ACNGT 3 cut(s) 277, 694, 895
Bst6I CTCTTC 2 cut(s) 765, 882
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 485, 1229
BstDEI CTNAG 4 cut(s) 372, 395, 459, 1319
BstDSI CCRYGG 4 cut(s) 242, 276, 598, 946
BstF5I GGATG 3 cut(s) 142, 608, 639
BstFNI CGCG 1 cut(s) 948
BstKTI GATC 6 cut(s) 241, 367, 466, 538, 1055, 1134
BstMAI GTCTC 4 cut(s) 773, 884, 919, 1034
BstMBI GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
BstMWI GCNNNNNNNGC 3 cut(s) 321, 1016, 1046
BstNI CCWGG 4 cut(s) 474, 653, 1137, 1167
BstSCI CCNGG 4 cut(s) 472, 651, 1135, 1165
BstSFI CTRYAG 1 cut(s) 1047
BstUI CGCG 1 cut(s) 948
BstV1I GCAGC 3 cut(s) 39, 499, 922
BstX2I RGATCY 2 cut(s) 463, 535
BstXI CCANNNNNNTGG 1 cut(s) 1381
BstYI RGATCY 2 cut(s) 463, 535
Bsu36I CCTNAGG 2 cut(s) 459, 1319
BsuRI GGCC 3 cut(s) 343, 618, 901
BtgI CCRYGG 4 cut(s) 242, 276, 598, 946
BtsCI GGATG 3 cut(s) 142, 608, 639
BtsIMutI CAGTG 2 cut(s) 699, 900
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 2 cut(s) 485, 1229
CaiI CAGNNNCTG 2 cut(s) 880, 959
Cfr13I GGNCC 8 cut(s) 35, 190, 617, 636, 671, 704, 800, 1371
Cfr42I CCGCGG 1 cut(s) 949
Csp6I GTAC 4 cut(s) 289, 497, 1091, 1218
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 7 cut(s) 243, 418, 599, 783, 1056, 1154, 1174
CviQI GTAC 4 cut(s) 289, 497, 1091, 1218
DdeI CTNAG 4 cut(s) 372, 395, 459, 1319
DpnI GATC 6 cut(s) 240, 366, 465, 537, 1054, 1133
DpnII GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 765, 882
EarI CTCTTC 2 cut(s) 765, 882
Eco130I CCWWGG 3 cut(s) 31, 242, 598
Eco31I GGTCTC 1 cut(s) 1034
Eco47I GGWCC 7 cut(s) 35, 190, 636, 671, 704, 800, 1371
Eco57I CTGAAG 2 cut(s) 1233, 1323
Eco81I CCTNAGG 2 cut(s) 459, 1319
EcoO109I RGGNCCY 2 cut(s) 35, 671
EcoRII CCWGG 4 cut(s) 472, 651, 1135, 1165
EcoT14I CCWWGG 3 cut(s) 31, 242, 598
EcoT22I ATGCAT 1 cut(s) 1301
ErhI CCWWGG 3 cut(s) 31, 242, 598
FaeI CATG 7 cut(s) 246, 421, 602, 786, 1059, 1157, 1177
FatI CATG 7 cut(s) 242, 417, 598, 782, 1055, 1153, 1173
Fnu4HI GCNGC 4 cut(s) 28, 341, 488, 936
FokI GGATG 3 cut(s) 149, 615, 626
Fsp4HI GCNGC 4 cut(s) 28, 341, 488, 936
FspBI CTAG 2 cut(s) 515, 1283
GluI GCNGC 4 cut(s) 28, 341, 488, 936
GsaI CCCAGC 1 cut(s) 1162
GsuI CTGGAG 1 cut(s) 1158
HaeIII GGCC 3 cut(s) 343, 618, 901
HapII CCGG 1 cut(s) 356
Hin1II CATG 7 cut(s) 246, 421, 602, 786, 1059, 1157, 1177
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 3 cut(s) 68, 392, 1229
HinfI GANTC 5 cut(s) 152, 503, 594, 1100, 1343
HpaII CCGG 1 cut(s) 356
HphI GGTGA 6 cut(s) 76, 188, 265, 912, 1181, 1190
Hpy166II GTNNAC 4 cut(s) 280, 553, 593, 697
Hpy188I TCNGA 6 cut(s) 375, 629, 648, 662, 1213, 1342
Hpy188III TCNNGA 6 cut(s) 134, 226, 533, 960, 1123, 1321
Hpy8I GTNNAC 4 cut(s) 280, 553, 593, 697
HpyAV CCTTC 7 cut(s) 140, 164, 232, 653, 784, 1196, 1208
HpyCH4III ACNGT 3 cut(s) 277, 694, 895
HpyCH4V TGCA 7 cut(s) 719, 866, 938, 976, 1040, 1049, 1299
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 1016, 1046
HpyF3I CTNAG 4 cut(s) 372, 395, 459, 1319
Hsp92II CATG 7 cut(s) 246, 421, 602, 786, 1059, 1157, 1177
KspI CCGCGG 1 cut(s) 949
Kzo9I GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
Lsp1109I GCAGC 3 cut(s) 39, 499, 922
LweI GCATC 3 cut(s) 127, 853, 1295
MaeI CTAG 2 cut(s) 515, 1283
MaeIII GTNAC 2 cut(s) 43, 918
MalI GATC 6 cut(s) 240, 366, 465, 537, 1054, 1133
MbiI CCGCTC 1 cut(s) 340
MboI GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
MboII GAAGA 5 cut(s) 22, 782, 799, 899, 975
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 2 cut(s) 463, 535
MluCI AATT 7 cut(s) 20, 75, 99, 330, 492, 764, 850
MlyI GAGTC 2 cut(s) 512, 588
MmeI TCCRAC 2 cut(s) 731, 1398
Mph1103I ATGCAT 1 cut(s) 1301
MseI TTAA 4 cut(s) 390, 819, 1116, 1293
MslI CAYNNNNRTG 1 cut(s) 1294
MspA1I CMGCKG 1 cut(s) 948
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 4 cut(s) 474, 653, 1137, 1167
MunI CAATTG 1 cut(s) 75
MvaI CCWGG 4 cut(s) 474, 653, 1137, 1167
MvnI CGCG 1 cut(s) 948
MwoI GCNNNNNNNGC 3 cut(s) 321, 1016, 1046
NcoI CCATGG 2 cut(s) 242, 598
NdeII GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
NlaIII CATG 7 cut(s) 246, 421, 602, 786, 1059, 1157, 1177
NlaIV GGNNCC 4 cut(s) 619, 802, 1164, 1275
NmuCI GTSAC 1 cut(s) 918
NsiI ATGCAT 1 cut(s) 1301
NspV TTCGAA 1 cut(s) 768
PfeI GAWTC 3 cut(s) 152, 1100, 1343
PflMI CCANNNNNTGG 2 cut(s) 418, 1368
PkrI GCNGC 4 cut(s) 29, 342, 489, 937
PleI GAGTC 2 cut(s) 511, 588
PpsI GAGTC 2 cut(s) 511, 588
PpuMI RGGWCCY 2 cut(s) 35, 671
Psp5II RGGWCCY 2 cut(s) 35, 671
Psp6I CCWGG 4 cut(s) 472, 651, 1135, 1165
PspFI CCCAGC 1 cut(s) 1158
PspGI CCWGG 4 cut(s) 472, 651, 1135, 1165
PspN4I GGNNCC 4 cut(s) 619, 802, 1164, 1275
PspPI GGNCC 8 cut(s) 35, 190, 617, 636, 671, 704, 800, 1371
PspPPI RGGWCCY 2 cut(s) 35, 671
PstI CTGCAG 1 cut(s) 1051
PstNI CAGNNNCTG 2 cut(s) 880, 959
PsuI RGATCY 2 cut(s) 463, 535
RsaI GTAC 4 cut(s) 290, 498, 1092, 1219
RsaNI GTAC 4 cut(s) 289, 497, 1091, 1218
RseI CAYNNNNRTG 1 cut(s) 1294
SacII CCGCGG 1 cut(s) 949
SaqAI TTAA 4 cut(s) 390, 819, 1116, 1293
SatI GCNGC 4 cut(s) 28, 341, 488, 936
Sau3AI GATC 6 cut(s) 238, 364, 463, 535, 1052, 1131
Sau96I GGNCC 8 cut(s) 35, 190, 617, 636, 671, 704, 800, 1371
SchI GAGTC 2 cut(s) 512, 588
ScrFI CCNGG 4 cut(s) 474, 653, 1137, 1167
SfaNI GCATC 3 cut(s) 127, 853, 1295
SfcI CTRYAG 1 cut(s) 1047
Sfr303I CCGCGG 1 cut(s) 949
SfuI TTCGAA 1 cut(s) 768
SgrBI CCGCGG 1 cut(s) 949
SinI GGWCC 7 cut(s) 35, 190, 636, 671, 704, 800, 1371
SmiMI CAYNNNNRTG 1 cut(s) 1294
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 7 cut(s) 20, 75, 99, 330, 492, 764, 850
SsiI CCGC 4 cut(s) 340, 946, 948, 1225
SspMI CTAG 2 cut(s) 515, 1283
StyD4I CCNGG 4 cut(s) 472, 651, 1135, 1165
StyI CCWWGG 3 cut(s) 31, 242, 598
TaaI ACNGT 3 cut(s) 277, 694, 895
TaqI TCGA 4 cut(s) 160, 253, 363, 768
TasI AATT 7 cut(s) 20, 75, 99, 330, 492, 764, 850
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 3 cut(s) 152, 1100, 1343
Tru1I TTAA 4 cut(s) 390, 819, 1116, 1293
Tru9I TTAA 4 cut(s) 390, 819, 1116, 1293
TscAI CASTG 2 cut(s) 706, 900
TseFI GTSAC 1 cut(s) 918
TseI GCWGC 3 cut(s) 27, 487, 935
Tsp45I GTSAC 1 cut(s) 918
TspDTI ATGAA 5 cut(s) 32, 165, 212, 1142, 1162
TspRI CASTG 2 cut(s) 706, 900
Van91I CCANNNNNTGG 2 cut(s) 418, 1368
VpaK11BI GGWCC 7 cut(s) 35, 190, 636, 671, 704, 800, 1371
XapI RAATTY 1 cut(s) 330
XspI CTAG 2 cut(s) 515, 1283
Zsp2I ATGCAT 1 cut(s) 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.