Rmu_sc0018892.1_g000001

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018892.1
OG0015567
Physical Location & Seq
Forward (+)
1 .. 1068
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018892.1_g000001.1.cds

Sequence Viewer

Length: 724 bp
gttccactacatggcaataacagatgagaaacaaagggtaatgcctatgcctgaggatctgcttccaggtagaggcaagcaactaattgtaccagagtcagttctgctagtaaatcccataaatccagatctcaaaggggaggttgacgataagtatatgtattcaatggataacaaagatagtggactccatggatggataggttctggccccattatcggattttggaccatccttccgagccaggagttcagaaatggaggacctacaaagcaaaacctcacagttcacactggtccaacctgccttgcaatgtttcagggaacacattatcttgggttagatatggtagctcaattcgaagagggagagacatggaagaaggtgtttggacccgtctttatataccttaactcaacaccagatacatcaaaagcccacaatttatggacagatgcaaagaaacagaggctgtttgaagagacagtgtggccttatgattttgtctcgtcacccaactatgttgctgcaaaagaccgcggttcagtttctggaagattgttagtgcaggacaggtatatttcgggttctctcattcctgctaaaaatgcttatattggtctctcggttgcaactactgcaggatcatggcaacttgaaagcaaggtagttactaaccagacgctaaatatgtacaggttgatcttccacttgtgcacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

240

Amino Acids

26.95

Weight (kDa)

6.33

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015567)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01850
malus_domestica MD10G1323400.v1.1
prunus_persica Prupe.4G018900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002721
rosa_laevigata RLG00000031054
rosa_multiflora Rmu_sc0018891.1_g000001 Rmu_sc0018892.1_g000001 Rmu_sc0037618.1_g000002 Rmu_sc0041618.1_g000001
rosa_roxburghii Rroxscaffold_1G00073160
rosa_rugosa Rorug04G0398700
rosa_samantha Rh5AG021900 Rh5BG024800 Rh5CG024200 Rh5DG023200
rosa_wichuraiana Rw5G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 312
AccB7I CCANNNNNTGG 1 cut(s) 11
AccII CGCG 1 cut(s) 541
AciI CCGC 2 cut(s) 539, 541
AclWI GGATC 2 cut(s) 64, 653
AfaI GTAC 2 cut(s) 91, 696
AfiI CCNNNNNNNGG 3 cut(s) 11, 72, 219
AgsI TTSAA 3 cut(s) 166, 480, 660
AjnI CCWGG 2 cut(s) 65, 244
AluBI AGCT 1 cut(s) 354
AluI AGCT 1 cut(s) 354
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 4 cut(s) 366, 477, 512, 627
Alw44I GTGCAC 1 cut(s) 716
AlwI GGATC 2 cut(s) 64, 653
AlwNI CAGNNNCTG 2 cut(s) 473, 552
AoxI GGCC 2 cut(s) 209, 492
ApaLI GTGCAC 1 cut(s) 716
ApeKI GCWGC 1 cut(s) 528
AspS9I GGNCC 5 cut(s) 210, 229, 264, 297, 393
AsuHPI GGTGA 1 cut(s) 505
AsuII TTCGAA 1 cut(s) 361
AvaII GGWCC 4 cut(s) 229, 264, 297, 393
AxyI CCTNAGG 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 1 cut(s) 515
BccI CCATC 2 cut(s) 190, 240
BciT130I CCWGG 2 cut(s) 67, 246
BcoDI GTCTC 4 cut(s) 366, 477, 512, 627
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 312
BglII AGATCT 1 cut(s) 128
BisI GCNGC 1 cut(s) 529
BlsI GCNGC 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 67, 246
Bme18I GGWCC 4 cut(s) 229, 264, 297, 393
BmgT120I GGNCC 5 cut(s) 210, 229, 264, 297, 393
BmiI GGNNCC 2 cut(s) 212, 395
BmrFI CCNGG 2 cut(s) 67, 246
BmsI GCATC 1 cut(s) 446
Bpu14I TTCGAA 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 191, 539
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 72, 219
Bse1I ACTGG 1 cut(s) 299
Bse21I CCTNAGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 2 cut(s) 67, 246
BseDI CCNNGG 2 cut(s) 191, 539
BseGI GGATG 2 cut(s) 201, 232
BseLI CCNNNNNNNGG 3 cut(s) 11, 72, 219
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 43
BseNI ACTGG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 1 cut(s) 515
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 211, 494
BsiHKAI GWGCWC 1 cut(s) 720
BslI CCNNNNNNNGG 3 cut(s) 11, 72, 219
BsmAI GTCTC 4 cut(s) 366, 477, 512, 627
BsnI GGCC 2 cut(s) 211, 494
Bso31I GGTCTC 1 cut(s) 627
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 720
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 4 cut(s) 56, 128, 645, 703
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 2 cut(s) 539, 541
BspANI GGCC 2 cut(s) 211, 494
BspCNI CTCAG 1 cut(s) 44
BspFNI CGCG 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 212, 395
BspMAI CTGCAG 1 cut(s) 644
BspMI ACCTGC 1 cut(s) 312
BspPI GGATC 2 cut(s) 64, 653
BspT104I TTCGAA 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 191, 539
BssMI GATC 4 cut(s) 56, 128, 645, 703
BssT1I CCWWGG 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 67, 246
Bst4CI ACNGT 2 cut(s) 287, 488
Bst6I CTCTTC 2 cut(s) 358, 475
BstAPI GCANNNNNTGC 1 cut(s) 639
BstAUI TGTACA 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 52
BstDSI CCRYGG 2 cut(s) 191, 539
BstF5I GGATG 2 cut(s) 201, 232
BstFNI CGCG 1 cut(s) 541
BstKTI GATC 4 cut(s) 59, 131, 648, 706
BstMAI GTCTC 4 cut(s) 366, 477, 512, 627
BstMBI GATC 4 cut(s) 56, 128, 645, 703
BstMWI GCNNNNNNNGC 2 cut(s) 609, 639
BstNI CCWGG 2 cut(s) 67, 246
BstSCI CCNGG 2 cut(s) 65, 244
BstSFI CTRYAG 1 cut(s) 640
BstSLI GKGCMC 1 cut(s) 720
BstUI CGCG 1 cut(s) 541
BstV1I GCAGC 1 cut(s) 515
BstX2I RGATCY 2 cut(s) 56, 128
BstYI RGATCY 2 cut(s) 56, 128
Bsu36I CCTNAGG 1 cut(s) 52
BsuRI GGCC 2 cut(s) 211, 494
BtgI CCRYGG 2 cut(s) 191, 539
BtsCI GGATG 2 cut(s) 201, 232
BtsIMutI CAGTG 2 cut(s) 292, 493
BveI ACCTGC 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 2 cut(s) 473, 552
Cfr13I GGNCC 5 cut(s) 210, 229, 264, 297, 393
Cfr42I CCGCGG 1 cut(s) 542
CseI GACGC 1 cut(s) 692
Csp6I GTAC 2 cut(s) 90, 695
CspCI CAANNNNNGTGG 2 cut(s) 164, 199
CviAII CATG 4 cut(s) 11, 192, 376, 649
CviJI RGCY 6 cut(s) 211, 244, 354, 438, 473, 494
CviKI_1 RGCY 6 cut(s) 211, 244, 354, 438, 473, 494
CviQI GTAC 2 cut(s) 90, 695
DdeI CTNAG 1 cut(s) 52
DpnI GATC 4 cut(s) 58, 130, 647, 705
DpnII GATC 4 cut(s) 56, 128, 645, 703
Eam1104I CTCTTC 2 cut(s) 358, 475
EarI CTCTTC 2 cut(s) 358, 475
Eco130I CCWWGG 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 627
Eco47I GGWCC 4 cut(s) 229, 264, 297, 393
Eco81I CCTNAGG 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 264
EcoRII CCWGG 2 cut(s) 65, 244
EcoT14I CCWWGG 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 4 cut(s) 14, 195, 379, 652
FatI CATG 4 cut(s) 10, 191, 375, 648
Fnu4HI GCNGC 1 cut(s) 529
FokI GGATG 2 cut(s) 208, 219
Fsp4HI GCNGC 1 cut(s) 529
FspBI CTAG 1 cut(s) 108
GluI GCNGC 1 cut(s) 529
HaeIII GGCC 2 cut(s) 211, 494
HgaI GACGC 1 cut(s) 692
Hin1II CATG 4 cut(s) 14, 195, 379, 652
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HinfI GANTC 2 cut(s) 96, 187
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 4 cut(s) 146, 186, 290, 718
Hpy188I TCNGA 3 cut(s) 222, 241, 255
Hpy188III TCNNGA 2 cut(s) 126, 553
Hpy8I GTNNAC 4 cut(s) 146, 186, 290, 718
HpyAV CCTTC 2 cut(s) 246, 377
HpyCH4III ACNGT 2 cut(s) 287, 488
HpyCH4V TGCA 7 cut(s) 312, 459, 531, 569, 633, 642, 718
HpyF10VI GCNNNNNNNGC 2 cut(s) 609, 639
HpyF3I CTNAG 1 cut(s) 52
Hsp92II CATG 4 cut(s) 14, 195, 379, 652
KspI CCGCGG 1 cut(s) 542
Kzo9I GATC 4 cut(s) 56, 128, 645, 703
Lsp1109I GCAGC 1 cut(s) 515
LweI GCATC 1 cut(s) 446
MaeI CTAG 1 cut(s) 108
MaeIII GTNAC 2 cut(s) 511, 671
MalI GATC 4 cut(s) 58, 130, 647, 705
MboI GATC 4 cut(s) 56, 128, 645, 703
MboII GAAGA 5 cut(s) 375, 392, 492, 568, 698
MflI RGATCY 2 cut(s) 56, 128
MhlI GDGCHC 1 cut(s) 720
MluCI AATT 3 cut(s) 85, 357, 443
MlyI GAGTC 2 cut(s) 105, 181
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 7 cut(s) 47, 66, 134, 255, 291, 359, 463
MseI TTAA 2 cut(s) 412, 722
MspA1I CMGCKG 1 cut(s) 541
MspR9I CCNGG 2 cut(s) 67, 246
MvaI CCWGG 2 cut(s) 67, 246
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 2 cut(s) 609, 639
NcoI CCATGG 1 cut(s) 191
NdeII GATC 4 cut(s) 56, 128, 645, 703
NlaIII CATG 4 cut(s) 14, 195, 379, 652
NlaIV GGNNCC 2 cut(s) 212, 395
NmuCI GTSAC 1 cut(s) 511
NspV TTCGAA 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 11
PkrI GCNGC 1 cut(s) 530
PleI GAGTC 2 cut(s) 104, 181
PpsI GAGTC 2 cut(s) 104, 181
PpuMI RGGWCCY 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 264
Psp6I CCWGG 2 cut(s) 65, 244
PspGI CCWGG 2 cut(s) 65, 244
PspN4I GGNNCC 2 cut(s) 212, 395
PspPI GGNCC 5 cut(s) 210, 229, 264, 297, 393
PspPPI RGGWCCY 1 cut(s) 264
PstI CTGCAG 1 cut(s) 644
PstNI CAGNNNCTG 2 cut(s) 473, 552
PsuI RGATCY 2 cut(s) 56, 128
RsaI GTAC 2 cut(s) 91, 696
RsaNI GTAC 2 cut(s) 90, 695
SacII CCGCGG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 412, 722
SatI GCNGC 1 cut(s) 529
Sau3AI GATC 4 cut(s) 56, 128, 645, 703
Sau96I GGNCC 5 cut(s) 210, 229, 264, 297, 393
SchI GAGTC 2 cut(s) 105, 181
ScrFI CCNGG 2 cut(s) 67, 246
SduI GDGCHC 1 cut(s) 720
SfaNI GCATC 1 cut(s) 446
SfcI CTRYAG 1 cut(s) 640
Sfr303I CCGCGG 1 cut(s) 542
SfuI TTCGAA 1 cut(s) 361
SgrBI CCGCGG 1 cut(s) 542
SinI GGWCC 4 cut(s) 229, 264, 297, 393
Sse9I AATT 3 cut(s) 85, 357, 443
SsiI CCGC 2 cut(s) 539, 541
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 65, 244
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 287, 488
TaqI TCGA 1 cut(s) 361
TasI AATT 3 cut(s) 85, 357, 443
TatI WGTACW 1 cut(s) 694
Tru1I TTAA 2 cut(s) 412, 722
Tru9I TTAA 2 cut(s) 412, 722
TscAI CASTG 2 cut(s) 299, 493
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 1 cut(s) 528
Tsp45I GTSAC 1 cut(s) 511
TspRI CASTG 2 cut(s) 299, 493
Van91I CCANNNNNTGG 1 cut(s) 11
VneI GTGCAC 1 cut(s) 716
VpaK11BI GGWCC 4 cut(s) 229, 264, 297, 393
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.