Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012083

Physical Location & Seq

Genomic Coordinates Forward (+)

41583463 .. 41586243

Gene Structure 2781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1340400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGTACGGTAGAGCGGGCCTTGATCGATTTAAGAAAGCTCAGTCCTTGGAACCCTTCTCCGTCAATTCCAATCCCAATCCCAAACCCAATTCCCCTTCCAAATCCAATCCCAAGCCCTCTCTTTCACACTCCCATCAACATCAACCCCCCCTCCCTCTCCCTCTTGCACCACCCGAGGCGGCCCCTCTCGTGGAGCTGGGTCAGACCCACCAGCACCATCTCCCTCAGCTTGGTGCCGGCCAGTCCACTTGGAGGCCCCCTGACTGGGCAATCGACCCCCGCCCTGGTGTCTATTCTCTCGAGGTTCTCAAGGATGGTCAGGTTCTTGATCGCATTGCCCTTGTTAGGCGTCGCAACATCTTTGGCCGCCAATCTCAGACTTGTGACTTTGTGCTTGATCATCAGTCCGTTTCCCGCCAGCATGCTGTTGTTGTTCCTCACAAGAACGGAAGCATATATGTAATTGATTTGGGGTCTGCACATGGTACTTTTGTTGCAAATGAAAGAGTGACTAAAGATACCCCTGTTGAACTTGAAGTGGGCCAGTCTTTGAGATTTGCTGCATCAACTAGAACTTATGTCTTGAGAAAGAATGACGCAGCTCTTTTTCCTCGTCCTCCACGGCCTTCAGAGGTTAATCTGCCACCACCTCCTGATCCATCTGATGAAGATGCTGTTGTGGCCTATAATACGCTTCTAAATCGTCATGGTCTGACCAACCCTGACGTAGTGGCTAAGTCCCGCGAGTCTAGTGACTCAGCAGGTGCAAAAGGAGACAACCAGCGGCCAGAGAGGCCCTCTAAGAGAATTAGGAAGGCTAGAGTATCATTCAGGGATCAAGTTGGAGGAGAGCTGGTTGAAGTAGTTGGGATTTCAGACGGTGTGGACGTAGAAACTGAACCGGGTCCAGTAGGTGTAAAAGAAGGAAGTCTTGTCGGGAAATATGAATCCCTTGTGCAGGTTACAGTAATCCCCAAAGGGAAGGAACAATTATATGTCAAGGAAGGCAATTCTTCCCAAAAAGGCATGACTGAAAAATTACAGGAGGTCTTAAATAGAGTCAAGACTGCTCCGAAGGGTAGGATTTATGATGAGCTTTATGGGGAATCTTTTTCTGGCAAAGTGGGTTCGTCATGGGCATATCCTTCTTCTGTGGACTCGAGTGGTAGAAGACCCTCTCCAAGTAGAGATACTGATGGGAAGGCATTAAGCGTAAACCATGGAACTAACTCAAGTCACATTGACGATGACGAAGATGATTTGTTTGGTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0004857 GO:0004864 GO:0004865 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0008150 GO:0009892 GO:0010563 GO:0010605 GO:0010921 GO:0010923 GO:0016604 GO:0016607 GO:0019208 GO:0019212 GO:0019220 GO:0019222 GO:0019888 GO:0030234 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032515 GO:0035303 GO:0035304 GO:0035305 GO:0035308 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043666 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045936 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0080090 GO:0097159 GO:0098772 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA

Protein Analysis

425

Amino Acids

46.27

Weight (kDa)

7.25

Isoelectric Point (pI)

44.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Yop-YscD_cpl	PF16697	114 - 183	2.8e-06	Inner membrane component of T3SS, cytoplasmic domain
FHA	PF00498	120 - 186	4.9e-19	FHA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012083)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47790
fragaria_vesca	FvH4_3g00210
malus_domestica	MD05G1362800.v1.1 MD10G1340400.v1.1
prunus_persica	Prupe.4G002500_v2.0.a1 Prupe.4G002500_v2.0.a1 Prupe.4G002500_v2.0.a1
pyrus_communis	pycom05g32930 pycom10g29020
rosa_chinensis	RchiOBHm_Chr5g0000281
rosa_laevigata	RLG00000030856
rosa_multiflora	Rmu_ssc0000385.1_g000005
rosa_roxburghii	Rroxscaffold_1G00075830
rosa_rugosa	Rorug04G0383000
rosa_samantha	Rh5AG002100 Rh5BG003500 Rh5CG002400 Rh5DG002400
rosa_wichuraiana	Rw5G000260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	752
Acc36I	ACCTGC	2 cut(s)	752, 949
AccB1I	GGYRCC	1 cut(s)	233
AccBSI	CCGCTC	1 cut(s)	14
AccII	CGCG	1 cut(s)	744
AciI	CCGC	7 cut(s)	14, 179, 280, 367, 415, 742, 784
AclWI	GGATC	2 cut(s)	650, 843
AcoI	YGGCCR	3 cut(s)	238, 364, 785
AcuI	CTGAAG	1 cut(s)	612
AcyI	GRCGYC	1 cut(s)	349
AfaI	GTAC	2 cut(s)	5, 487
AfiI	CCNNNNNNNGG	8 cut(s)	190, 230, 252, 264, 265, 284, 345, 958
AgsI	TTSAA	3 cut(s)	530, 536, 860
AjnI	CCWGG	1 cut(s)	283
AluBI	AGCT	6 cut(s)	38, 196, 229, 602, 853, 1096
AluI	AGCT	6 cut(s)	38, 196, 229, 602, 853, 1096
Alw26I	GTCTC	1 cut(s)	768
AlwI	GGATC	2 cut(s)	650, 843
Ama87I	CYCGRG	3 cut(s)	173, 299, 1159
ApeKI	GCWGC	2 cut(s)	560, 599
AspS9I	GGNCC	6 cut(s)	16, 181, 255, 541, 795, 905
AsuC2I	CCSGG	1 cut(s)	903
AvaI	CYCGRG	3 cut(s)	173, 299, 1159
AvaII	GGWCC	1 cut(s)	905
BaeI	ACNNNNGTAYC	4 cut(s)	477, 510, 510, 543
BanI	GGYRCC	1 cut(s)	233
BauI	CACGAG	1 cut(s)	188
BbsI	GAAGAC	1 cut(s)	1177
BbvCI	CCTCAGC	1 cut(s)	225
BbvI	GCAGC	2 cut(s)	547, 611
BccI	CCATC	5 cut(s)	141, 225, 308, 667, 1190
BceAI	ACGGC	1 cut(s)	638
BciT130I	CCWGG	1 cut(s)	285
BclI	TGATCA	1 cut(s)	397
BcnI	CCSGG	1 cut(s)	903
BcoDI	GTCTC	1 cut(s)	768
BfaI	CTAG	3 cut(s)	570, 750, 819
BfuAI	ACCTGC	2 cut(s)	752, 949
BglI	GCCNNNNNGGC	1 cut(s)	793
BisI	GCNGC	5 cut(s)	180, 367, 561, 600, 785
BlsI	GCNGC	5 cut(s)	181, 368, 562, 601, 786
Bme1390I	CCNGG	2 cut(s)	285, 903
Bme18I	GGWCC	1 cut(s)	905
BmeT110I	CYCGRG	3 cut(s)	173, 299, 1159
BmgT120I	GGNCC	6 cut(s)	16, 181, 255, 541, 795, 905
BmiI	GGNNCC	5 cut(s)	51, 183, 235, 257, 906
BmrFI	CCNGG	2 cut(s)	285, 903
BmrI	ACTGGG	1 cut(s)	274
BmsI	GCATC	2 cut(s)	572, 661
BmuI	ACTGGG	1 cut(s)	274
BpiI	GAAGAC	1 cut(s)	1177
BplI	GAGNNNNNCTC	2 cut(s)	782, 814
Bpu10I	CCTNAGC	1 cut(s)	225
BpuEI	CTTGAG	3 cut(s)	293, 604, 1216
BpuMI	CCSGG	1 cut(s)	903
Bsa29I	ATCGAT	1 cut(s)	25
BsaHI	GRCGYC	1 cut(s)	349
BsaJI	CCNNGG	5 cut(s)	45, 174, 283, 620, 1219
BsaXI	ACNNNNNCTCC	4 cut(s)	135, 165, 840, 870
Bsc4I	CCNNNNNNNGG	8 cut(s)	190, 230, 252, 264, 265, 284, 345, 958
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	4 cut(s)	241, 269, 544, 908
Bse3DI	GCAATG	1 cut(s)	333
BseBI	CCWGG	1 cut(s)	285
BseCI	ATCGAT	1 cut(s)	25
BseDI	CCNNGG	5 cut(s)	45, 174, 283, 620, 1219
BseGI	GGATG	1 cut(s)	319
BseLI	CCNNNNNNNGG	8 cut(s)	190, 230, 252, 264, 265, 284, 345, 958
BseMI	GCAATG	1 cut(s)	333
BseMII	CTCAG	4 cut(s)	53, 239, 389, 771
BseNI	ACTGG	4 cut(s)	241, 269, 544, 908
BseRI	GAGGAG	1 cut(s)	861
BseXI	GCAGC	2 cut(s)	547, 611
BseYI	CCCAGC	1 cut(s)	196
BsgI	GTGCAG	2 cut(s)	462, 977
Bsh1236I	CGCG	1 cut(s)	744
BshNI	GGYRCC	1 cut(s)	233
BshVI	ATCGAT	1 cut(s)	25
BsiHKCI	CYCGRG	3 cut(s)	173, 299, 1159
BsiSI	CCGG	2 cut(s)	237, 902
BslFI	GGGAC	1 cut(s)	724
BslI	CCNNNNNNNGG	8 cut(s)	190, 230, 252, 264, 265, 284, 345, 958
BsmAI	GTCTC	1 cut(s)	768
BsmFI	GGGAC	1 cut(s)	724
BsoBI	CYCGRG	3 cut(s)	173, 299, 1159
Bsp143I	GATC	5 cut(s)	22, 328, 397, 655, 835
Bsp19I	CCATGG	1 cut(s)	1219
BspACI	CCGC	7 cut(s)	14, 179, 280, 367, 415, 742, 784
BspCNI	CTCAG	4 cut(s)	52, 238, 388, 770
BspDI	ATCGAT	1 cut(s)	25
BspFNI	CGCG	1 cut(s)	744
BspLI	GGNNCC	5 cut(s)	51, 183, 235, 257, 906
BspMI	ACCTGC	2 cut(s)	752, 949
BspPI	GGATC	2 cut(s)	650, 843
BspT107I	GGYRCC	1 cut(s)	233
BsrBI	CCGCTC	1 cut(s)	14
BsrDI	GCAATG	1 cut(s)	333
BsrFI	RCCGGY	1 cut(s)	236
BsrI	ACTGG	4 cut(s)	241, 269, 544, 908
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	5 cut(s)	45, 174, 283, 620, 1219
BssMI	GATC	5 cut(s)	22, 328, 397, 655, 835
BssNI	GRCGYC	1 cut(s)	349
BssSI	CACGAG	1 cut(s)	188
BssT1I	CCWWGG	2 cut(s)	45, 1219
Bst2BI	CACGAG	1 cut(s)	188
Bst2UI	CCWGG	1 cut(s)	285
Bst4CI	ACNGT	3 cut(s)	8, 881, 967
BstACI	GRCGYC	1 cut(s)	349
BstC8I	GCNNGC	4 cut(s)	16, 238, 419, 423
BstDEI	CTNAG	6 cut(s)	39, 225, 375, 735, 757, 801
BstDSI	CCRYGG	2 cut(s)	620, 1219
BstENI	CCTNNNNNAGG	1 cut(s)	956
BstF5I	GGATG	1 cut(s)	319
BstFNI	CGCG	1 cut(s)	744
BstKTI	GATC	5 cut(s)	25, 331, 400, 658, 838
BstMAI	GTCTC	1 cut(s)	768
BstMBI	GATC	5 cut(s)	22, 328, 397, 655, 835
BstMWI	GCNNNNNNNGC	2 cut(s)	680, 793
BstNI	CCWGG	1 cut(s)	285
BstNSI	RCATGY	1 cut(s)	425
BstSCI	CCNGG	2 cut(s)	283, 901
BstUI	CGCG	1 cut(s)	744
BstV1I	GCAGC	2 cut(s)	547, 611
BstV2I	GAAGAC	1 cut(s)	1177
Bsu15I	ATCGAT	1 cut(s)	25
BsuTUI	ATCGAT	1 cut(s)	25
BtgI	CCRYGG	2 cut(s)	620, 1219
BtsCI	GGATG	1 cut(s)	319
BveI	ACCTGC	2 cut(s)	752, 949
Cac8I	GCNNGC	4 cut(s)	16, 238, 419, 423
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	6 cut(s)	16, 181, 255, 541, 795, 905
ClaI	ATCGAT	1 cut(s)	25
CseI	GACGC	2 cut(s)	338, 605
Csp6I	GTAC	2 cut(s)	4, 486
CviAII	CATG	6 cut(s)	422, 482, 707, 1027, 1134, 1220
CviQI	GTAC	2 cut(s)	4, 486
DdeI	CTNAG	6 cut(s)	39, 225, 375, 735, 757, 801
DpnI	GATC	5 cut(s)	24, 330, 399, 657, 837
DpnII	GATC	5 cut(s)	22, 328, 397, 655, 835
EaeI	YGGCCR	3 cut(s)	238, 364, 785
Eco130I	CCWWGG	2 cut(s)	45, 1219
Eco47I	GGWCC	1 cut(s)	905
Eco57I	CTGAAG	1 cut(s)	612
Eco88I	CYCGRG	3 cut(s)	173, 299, 1159
EcoNI	CCTNNNNNAGG	1 cut(s)	956
EcoO109I	RGGNCCY	2 cut(s)	255, 795
EcoRII	CCWGG	1 cut(s)	283
EcoT14I	CCWWGG	2 cut(s)	45, 1219
ErhI	CCWWGG	2 cut(s)	45, 1219
FaeI	CATG	6 cut(s)	425, 485, 710, 1030, 1137, 1223
FalI	AAGNNNNNCTT	2 cut(s)	915, 947
FaqI	GGGAC	1 cut(s)	724
FatI	CATG	6 cut(s)	421, 481, 706, 1026, 1133, 1219
FauI	CCCGC	4 cut(s)	7, 287, 422, 749
FbaI	TGATCA	1 cut(s)	397
Fnu4HI	GCNGC	5 cut(s)	180, 367, 561, 600, 785
FokI	GGATG	1 cut(s)	326
Fsp4HI	GCNGC	5 cut(s)	180, 367, 561, 600, 785
FspBI	CTAG	3 cut(s)	570, 750, 819
GluI	GCNGC	5 cut(s)	180, 367, 561, 600, 785
GsaI	CCCAGC	1 cut(s)	200
HapII	CCGG	2 cut(s)	237, 902
HgaI	GACGC	2 cut(s)	338, 605
Hin1I	GRCGYC	1 cut(s)	349
Hin1II	CATG	6 cut(s)	425, 485, 710, 1030, 1137, 1223
HinfI	GANTC	6 cut(s)	746, 755, 947, 1059, 1106, 1157
HpaII	CCGG	2 cut(s)	237, 902
Hpy166II	GTNNAC	4 cut(s)	246, 886, 1156, 1216
Hpy188I	TCNGA	7 cut(s)	204, 378, 631, 664, 714, 877, 1074
Hpy188III	TCNNGA	6 cut(s)	299, 326, 583, 653, 937, 1063
Hpy8I	GTNNAC	4 cut(s)	246, 886, 1156, 1216
Hpy99I	CGWCG	1 cut(s)	354
HpyCH4III	ACNGT	3 cut(s)	8, 881, 967
HpyCH4IV	ACGT	2 cut(s)	726, 888
HpyCH4V	TGCA	6 cut(s)	167, 479, 497, 563, 767, 958
HpyF10VI	GCNNNNNNNGC	2 cut(s)	680, 793
HpyF3I	CTNAG	6 cut(s)	39, 225, 375, 735, 757, 801
HpySE526I	ACGT	2 cut(s)	726, 888
Hsp92I	GRCGYC	1 cut(s)	349
Hsp92II	CATG	6 cut(s)	425, 485, 710, 1030, 1137, 1223
KroI	GCCGGC	1 cut(s)	236
KroNI	GCCGGC	1 cut(s)	238
Ksp22I	TGATCA	1 cut(s)	397
Kzo9I	GATC	5 cut(s)	22, 328, 397, 655, 835
LmnI	GCTCC	2 cut(s)	193, 1075
Lsp1109I	GCAGC	2 cut(s)	547, 611
LweI	GCATC	2 cut(s)	572, 661
MaeI	CTAG	3 cut(s)	570, 750, 819
MaeII	ACGT	2 cut(s)	726, 888
MaeIII	GTNAC	5 cut(s)	383, 508, 752, 961, 1235
MalI	GATC	5 cut(s)	24, 330, 399, 657, 837
MbiI	CCGCTC	1 cut(s)	14
MboI	GATC	5 cut(s)	22, 328, 397, 655, 835
MboII	GAAGA	5 cut(s)	680, 1005, 1140, 1182, 1265
MluCI	AATT	7 cut(s)	64, 88, 462, 807, 989, 1009, 1037
MlyI	GAGTC	4 cut(s)	749, 755, 1068, 1151
MmeI	TCCRAC	1 cut(s)	823
MroNI	GCCGGC	1 cut(s)	236
MseI	TTAA	4 cut(s)	30, 636, 1052, 1208
MspA1I	CMGCKG	1 cut(s)	784
MspI	CCGG	2 cut(s)	237, 902
MspR9I	CCNGG	2 cut(s)	285, 903
MvaI	CCWGG	1 cut(s)	285
MvnI	CGCG	1 cut(s)	744
MwoI	GCNNNNNNNGC	2 cut(s)	680, 793
NaeI	GCCGGC	1 cut(s)	238
NciI	CCSGG	1 cut(s)	903
NcoI	CCATGG	1 cut(s)	1219
NdeII	GATC	5 cut(s)	22, 328, 397, 655, 835
NgoMIV	GCCGGC	1 cut(s)	236
NlaIII	CATG	6 cut(s)	425, 485, 710, 1030, 1137, 1223
NlaIV	GGNNCC	5 cut(s)	51, 183, 235, 257, 906
NmuCI	GTSAC	4 cut(s)	383, 508, 752, 1235
NspI	RCATGY	1 cut(s)	425
PaeI	GCATGC	1 cut(s)	425
PaeR7I	CTCGAG	2 cut(s)	299, 1159
PaqCI	CACCTGC	1 cut(s)	752
PcsI	WCGNNNNNNNCGW	1 cut(s)	885
PdiI	GCCGGC	1 cut(s)	238
PfeI	GAWTC	2 cut(s)	947, 1106
PkrI	GCNGC	5 cut(s)	181, 368, 562, 601, 786
PleI	GAGTC	4 cut(s)	749, 754, 1067, 1151
PpsI	GAGTC	4 cut(s)	749, 754, 1067, 1151
Psp6I	CCWGG	1 cut(s)	283
PspFI	CCCAGC	1 cut(s)	196
PspGI	CCWGG	1 cut(s)	283
PspN4I	GGNNCC	5 cut(s)	51, 183, 235, 257, 906
PspPI	GGNCC	6 cut(s)	16, 181, 255, 541, 795, 905
PspXI	VCTCGAGB	1 cut(s)	1159
RsaI	GTAC	2 cut(s)	5, 487
RsaNI	GTAC	2 cut(s)	4, 486
SaqAI	TTAA	4 cut(s)	30, 636, 1052, 1208
SatI	GCNGC	5 cut(s)	180, 367, 561, 600, 785
Sau3AI	GATC	5 cut(s)	22, 328, 397, 655, 835
Sau96I	GGNCC	6 cut(s)	16, 181, 255, 541, 795, 905
SchI	GAGTC	4 cut(s)	749, 755, 1068, 1151
ScrFI	CCNGG	2 cut(s)	285, 903
SfaNI	GCATC	2 cut(s)	572, 661
SfiI	GGCCNNNNNGGCC	1 cut(s)	793
Sfr274I	CTCGAG	2 cut(s)	299, 1159
SinI	GGWCC	1 cut(s)	905
SlaI	CTCGAG	2 cut(s)	299, 1159
SmlI	CTYRAG	5 cut(s)	299, 308, 583, 1159, 1231
SmoI	CTYRAG	5 cut(s)	299, 308, 583, 1159, 1231
SphI	GCATGC	1 cut(s)	425
Sse9I	AATT	7 cut(s)	64, 88, 462, 807, 989, 1009, 1037
SsiI	CCGC	7 cut(s)	14, 179, 280, 367, 415, 742, 784
SspMI	CTAG	3 cut(s)	570, 750, 819
StyD4I	CCNGG	2 cut(s)	283, 901
StyI	CCWWGG	2 cut(s)	45, 1219
TaaI	ACNGT	3 cut(s)	8, 881, 967
TaiI	ACGT	2 cut(s)	729, 891
TaqI	TCGA	4 cut(s)	25, 273, 300, 1160
TasI	AATT	7 cut(s)	64, 88, 462, 807, 989, 1009, 1037
TauI	GCSGC	3 cut(s)	182, 369, 787
TfiI	GAWTC	2 cut(s)	947, 1106
Tru1I	TTAA	4 cut(s)	30, 636, 1052, 1208
Tru9I	TTAA	4 cut(s)	30, 636, 1052, 1208
TseFI	GTSAC	4 cut(s)	383, 508, 752, 1235
TseI	GCWGC	2 cut(s)	560, 599
Tsp45I	GTSAC	4 cut(s)	383, 508, 752, 1235
TspDTI	ATGAA	3 cut(s)	516, 681, 960
TspGWI	ACGGA	3 cut(s)	49, 397, 462
VpaK11BI	GGWCC	1 cut(s)	905
XagI	CCTNNNNNAGG	1 cut(s)	956
XceI	RCATGY	1 cut(s)	425
XhoI	CTCGAG	2 cut(s)	299, 1159
XspI	CTAG	3 cut(s)	570, 750, 819

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.