MD11G1006400.v1.1

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009564
Physical Location & Seq
Forward (+)
615608 .. 617686
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1006400.v1.1.491

Sequence Viewer

Length: 522 bp
ATGCGAGGCAAGGTGGGTTGGAGACTTTCGATCGGCTGTTGCTTTTGCACTGCTGCTCCGAGGCCATGGCTATTTGTAGGTTTGGGCAATCCCGGCGATAAGTACCAAGGAACTAGGCACAATATGATTGATGCGTTTGCTGGTTCGCAGGGCATTGCATTGAACACGGTCCAGTGCAAAGCTATACTTGGGCAAGGTTTCGTGGGTGAAGTTCTGGTTTTTCTTGCAAAGCCTCAAATTGAAATGAATTTGAGAGGTGAATCTGACCCCCTTGCTGCTTATTATAAGCTCTCTCTCAATCGTGTGCTTGTGTTTCATGATGATATGTCCTCACCGTGTGGGGTACTTCGTCTTTACCCAAATGGAGGTCATGTAGACCACAGTGTGGGCATGTTGAATATCTTGCCAGGTATCGGGACGCCTCTTGGTCAAATGGATCCAAAAGCATTCTTGCTGCAAAAATTTAATGCAACAGCTCAAAGACGAGTGATTATATGGTGGTGGTGTTTTTGTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

174

Amino Acids

19.1

Weight (kDa)

9.21

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 24 - 158 1.6e-24 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AccB7I CCANNNNNTGG 1 cut(s) 385
AccI GTMKAC 1 cut(s) 375
AclWI GGATC 2 cut(s) 431, 444
AcsI RAATTY 2 cut(s) 247, 461
AcyI GRCGYC 1 cut(s) 419
AdeI CACNNNGTG 2 cut(s) 338, 385
AfaI GTAC 2 cut(s) 104, 345
AfiI CCNNNNNNNGG 3 cut(s) 365, 385, 413
AgsI TTSAA 3 cut(s) 163, 242, 397
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 3 cut(s) 182, 289, 476
AluI AGCT 3 cut(s) 182, 289, 476
Alw26I GTCTC 2 cut(s) 16, 518
AlwI GGATC 2 cut(s) 431, 444
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 3 cut(s) 53, 275, 454
ApoI RAATTY 2 cut(s) 247, 461
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 3 cut(s) 218, 269, 324
AvaII GGWCC 1 cut(s) 169
BamHI GGATCC 1 cut(s) 436
BbvI GCAGC 3 cut(s) 40, 262, 441
BciT130I CCWGG 1 cut(s) 408
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 16, 518
BfaI CTAG 1 cut(s) 114
BisI GCNGC 3 cut(s) 54, 276, 455
BlsI GCNGC 3 cut(s) 55, 277, 456
Bme1390I CCNGG 2 cut(s) 93, 408
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 93, 408
BmsI GCATC 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 59, 65, 106
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 357, 387
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 385, 413
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 408
BseDI CCNNGG 3 cut(s) 59, 65, 106
BseLI CCNNNNNNNGG 3 cut(s) 365, 385, 413
BseMI GCAATG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 40, 262, 441
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 64
BsiEI CGRYCG 1 cut(s) 33
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 3 cut(s) 365, 385, 413
BsmAI GTCTC 2 cut(s) 16, 518
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 64
Bsp143I GATC 2 cut(s) 30, 436
Bsp19I CCATGG 1 cut(s) 65
BspANI GGCC 1 cut(s) 64
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 438
BspPI GGATC 2 cut(s) 431, 444
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 59, 65, 106
BssMI GATC 2 cut(s) 30, 436
BssNI GRCGYC 1 cut(s) 419
BssT1I CCWWGG 2 cut(s) 65, 106
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 169, 336, 383
BstACI GRCGYC 1 cut(s) 419
BstDSI CCRYGG 1 cut(s) 65
BstKTI GATC 2 cut(s) 33, 439
BstMAI GTCTC 2 cut(s) 16, 518
BstMBI GATC 2 cut(s) 30, 436
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 2 cut(s) 91, 406
BstV1I GCAGC 3 cut(s) 40, 262, 441
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 48, 179, 388
CciI TCATGA 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 103, 344
CviAII CATG 4 cut(s) 66, 317, 371, 391
CviJI RGCY 7 cut(s) 36, 64, 70, 182, 232, 289, 476
CviKI_1 RGCY 7 cut(s) 36, 64, 70, 182, 232, 289, 476
CviQI GTAC 2 cut(s) 103, 344
DpnI GATC 2 cut(s) 32, 438
DpnII GATC 2 cut(s) 30, 436
DraIII CACNNNGTG 2 cut(s) 338, 385
Eco130I CCWWGG 2 cut(s) 65, 106
Eco47I GGWCC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 2 cut(s) 65, 106
ErhI CCWWGG 2 cut(s) 65, 106
FaeI CATG 4 cut(s) 69, 320, 374, 394
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 1 cut(s) 430
FatI CATG 4 cut(s) 65, 316, 370, 390
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 3 cut(s) 54, 276, 455
Fsp4HI GCNGC 3 cut(s) 54, 276, 455
FspBI CTAG 1 cut(s) 114
GluI GCNGC 3 cut(s) 54, 276, 455
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 427
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 4 cut(s) 69, 320, 374, 394
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 93
HphI GGTGA 3 cut(s) 218, 269, 324
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 60, 265
Hpy188III TCNNGA 2 cut(s) 317, 415
Hpy8I GTNNAC 1 cut(s) 376
HpyCH4III ACNGT 3 cut(s) 169, 336, 383
HpyCH4V TGCA 6 cut(s) 48, 158, 177, 227, 457, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 4 cut(s) 69, 320, 374, 394
Kzo9I GATC 2 cut(s) 30, 436
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 7 cut(s) 106, 126, 134, 185, 200, 393, 420
Lsp1109I GCAGC 3 cut(s) 40, 262, 441
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 114
MalI GATC 2 cut(s) 32, 438
MboI GATC 2 cut(s) 30, 436
MflI RGATCY 1 cut(s) 436
MluCI AATT 3 cut(s) 237, 247, 461
MnlI CCTC 6 cut(s) 54, 243, 248, 340, 359, 432
MseI TTAA 1 cut(s) 465
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 93, 408
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 65
NdeII GATC 2 cut(s) 30, 436
NlaIII CATG 4 cut(s) 69, 320, 374, 394
NlaIV GGNNCC 1 cut(s) 438
NspI RCATGY 1 cut(s) 394
PagI TCATGA 1 cut(s) 316
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 260
PflMI CCANNNNNTGG 1 cut(s) 385
PkrI GCNGC 3 cut(s) 55, 277, 456
Ple19I CGATCG 1 cut(s) 33
PsiI TTATAA 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 1 cut(s) 169
PsuI RGATCY 1 cut(s) 436
PvuI CGATCG 1 cut(s) 33
RsaI GTAC 2 cut(s) 104, 345
RsaNI GTAC 2 cut(s) 103, 344
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 3 cut(s) 54, 276, 455
Sau3AI GATC 2 cut(s) 30, 436
Sau96I GGNCC 1 cut(s) 169
ScrFI CCNGG 2 cut(s) 93, 408
SetI ASST 9 cut(s) 15, 82, 184, 199, 259, 291, 370, 412, 478
SfaNI GCATC 1 cut(s) 121
SinI GGWCC 1 cut(s) 169
Sse9I AATT 3 cut(s) 237, 247, 461
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 91, 406
StyI CCWWGG 2 cut(s) 65, 106
TaaI ACNGT 3 cut(s) 169, 336, 383
TaqI TCGA 1 cut(s) 29
TasI AATT 3 cut(s) 237, 247, 461
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 3 cut(s) 55, 179, 388
TseI GCWGC 3 cut(s) 53, 275, 454
TspDTI ATGAA 2 cut(s) 260, 305
TspRI CASTG 3 cut(s) 55, 179, 388
Van91I CCANNNNNTGG 1 cut(s) 385
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 2 cut(s) 247, 461
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.