Rh5BG560400

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009564
Physical Location & Seq
Reverse (-)
88944599 .. 88947904
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG560400.1

Sequence Viewer

Length: 534 bp
ATGATTAGTAGGTTAAGTAGGCGTTACTTTTGCTCTGATGCTCCGCGGCCGTGGCTGTTTGTGGGTTTGGGCAATCCCGGCGATAAGTACAAGGCAACCAGACACAATGTGGGGTTTGAGATGATTGATGTTTTCGCTCAATCGCAAGGGATAGCCATGAATAGAGTCCATTGCAAAGCTATATTCGGGCAAGGTTTTGTAGATGAAGTCCCTGTTTTCCTAGCAAAGCCTCAAACGTACATGAACCTGAGTGGTGAATCGACGGGACCGCTTGCTGCTTATTATAAGCTACCTCTCAATCGTGTGCTAGTGTTCCATGATGACATGAACTTACCGTGTGGGGTACTCCGTCTTAACCCAAATGGAGGTCATGGAAGCCACAGTGGGCTGAAGAGTGTGTTCTATAATTTTCGAGGGAACAGAGAATTTCCTCGTCTAAGAATTGGTATTGGAAGGCCTCCTGGTCAAATGGATCCAAAAGCATATCTGCTCCAAAAATTTAATGCAACAGCTCAAGGACGAGTAAGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

177

Amino Acids

19.78

Weight (kDa)

9.96

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 19 - 171 6.1e-55 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 46, 269
AclWI GGATC 2 cut(s) 467, 480
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 2 cut(s) 425, 497
AcuI CTGAAG 1 cut(s) 410
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 3 cut(s) 89, 239, 345
AfiI CCNNNNNNNGG 1 cut(s) 365
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 3 cut(s) 179, 289, 512
AluI AGCT 3 cut(s) 179, 289, 512
AlwI GGATC 2 cut(s) 467, 480
AoxI GGCC 2 cut(s) 47, 455
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 2 cut(s) 425, 497
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 266
BamHI GGATCC 1 cut(s) 472
BbvI GCAGC 1 cut(s) 262
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 462
BcnI CCSGG 1 cut(s) 78
BfaI CTAG 2 cut(s) 221, 308
BisI GCNGC 2 cut(s) 47, 276
BlsI GCNGC 2 cut(s) 48, 277
Bme1390I CCNGG 2 cut(s) 78, 462
Bme18I GGWCC 1 cut(s) 266
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 2 cut(s) 267, 474
BmrFI CCNGG 2 cut(s) 78, 462
BmsI GCATC 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 498
BpuMI CCSGG 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 44, 50
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 44, 50
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 239
BseX3I CGGCCG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 46
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 49, 457
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 2 cut(s) 194, 279
BslI CCNNNNNNNGG 1 cut(s) 365
BsmFI GGGAC 2 cut(s) 194, 279
BsnI GGCC 2 cut(s) 49, 457
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 3 cut(s) 44, 46, 269
BspANI GGCC 2 cut(s) 49, 457
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 267, 474
BspPI GGATC 2 cut(s) 467, 480
BsrDI GCAATG 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 44, 50
BssMI GATC 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 336, 383
Bst6I CTCTTC 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 248, 437
BstDSI CCRYGG 2 cut(s) 44, 50
BstFNI CGCG 1 cut(s) 46
BstKTI GATC 1 cut(s) 475
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 2 cut(s) 52, 78
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 2 cut(s) 76, 460
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BstZI CGGCCG 1 cut(s) 47
BsuRI GGCC 2 cut(s) 49, 457
BtgI CCRYGG 2 cut(s) 44, 50
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 266
Cfr42I CCGCGG 1 cut(s) 47
Csp6I GTAC 3 cut(s) 88, 238, 344
CviAII CATG 5 cut(s) 157, 241, 317, 325, 371
CviQI GTAC 3 cut(s) 88, 238, 344
DdeI CTNAG 2 cut(s) 248, 437
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 47
EagI CGGCCG 1 cut(s) 47
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EclXI CGGCCG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 266
Eco52I CGGCCG 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 5 cut(s) 160, 244, 320, 328, 374
FaqI GGGAC 2 cut(s) 194, 279
FatI CATG 5 cut(s) 156, 240, 316, 324, 370
Fnu4HI GCNGC 2 cut(s) 47, 276
Fsp4HI GCNGC 2 cut(s) 47, 276
FspBI CTAG 2 cut(s) 221, 308
GluI GCNGC 2 cut(s) 47, 276
HaeIII GGCC 2 cut(s) 49, 457
HapII CCGG 1 cut(s) 78
Hin1II CATG 5 cut(s) 160, 244, 320, 328, 374
HinfI GANTC 2 cut(s) 165, 257
HpaII CCGG 1 cut(s) 78
HphI GGTGA 1 cut(s) 266
Hpy188I TCNGA 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 2 cut(s) 336, 383
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 2 cut(s) 174, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 78
HpyF3I CTNAG 2 cut(s) 248, 437
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 5 cut(s) 160, 244, 320, 328, 374
KspI CCGCGG 1 cut(s) 47
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 2 cut(s) 46, 495
LpnPI CCDG 6 cut(s) 91, 112, 225, 260, 447, 474
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 28
MaeI CTAG 2 cut(s) 221, 308
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 1 cut(s) 23
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 1 cut(s) 403
MflI RGATCY 1 cut(s) 472
MluCI AATT 4 cut(s) 406, 425, 441, 497
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 6 cut(s) 240, 303, 359, 407, 441, 468
MseI TTAA 3 cut(s) 14, 354, 501
MspA1I CMGCKG 1 cut(s) 46
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 2 cut(s) 78, 462
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 52, 78
NciI CCSGG 1 cut(s) 78
NdeII GATC 1 cut(s) 472
NlaIII CATG 5 cut(s) 160, 244, 320, 328, 374
NlaIV GGNNCC 2 cut(s) 267, 474
PceI AGGCCT 1 cut(s) 457
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 48, 277
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PsiI TTATAA 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 267, 474
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 3 cut(s) 89, 239, 345
RsaNI GTAC 3 cut(s) 88, 238, 344
SacII CCGCGG 1 cut(s) 47
SaqAI TTAA 3 cut(s) 14, 354, 501
SatI GCNGC 2 cut(s) 47, 276
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 2 cut(s) 78, 462
SetI ASST 9 cut(s) 14, 181, 196, 239, 249, 291, 295, 370, 514
SfaNI GCATC 1 cut(s) 28
Sfr303I CCGCGG 1 cut(s) 47
SgrBI CCGCGG 1 cut(s) 47
SinI GGWCC 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 4 cut(s) 406, 425, 441, 497
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 3 cut(s) 44, 46, 269
SspMI CTAG 2 cut(s) 221, 308
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 76, 460
TaaI ACNGT 2 cut(s) 336, 383
TaiI ACGT 1 cut(s) 239
TaqI TCGA 2 cut(s) 260, 412
TasI AATT 4 cut(s) 406, 425, 441, 497
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 49
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 3 cut(s) 14, 354, 501
Tru9I TTAA 3 cut(s) 14, 354, 501
TscAI CASTG 1 cut(s) 388
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 4 cut(s) 173, 219, 257, 341
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 2 cut(s) 425, 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 2 cut(s) 221, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.