MD11G1006500.v1.1
MYB Family

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015126
Physical Location & Seq
Forward (+)
619660 .. 621027
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1006500.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGAGGAACCCATCGTCTTCGTCGAAAGCAGCAGCAGCAGCAAGTGCTAAGATGCAAACGACGATAACAGCGTCGTCCTCGTCGAGCAAGGCGGCTGGGGTTGCTGGAGGGACCAAGACGCCGTGTTGCGCAAAGGTGGGTTTGAAGAGAGGGCCTTGGACTCCCGAAGAGGACGAGCTGCTGGCAAATTACATCAAGAAAGAAGGGGAGGGACGGTGGCGGACCCTTCCCAAGCGGGCTGGGTTGCTCCGCTGCGGTAAGAGCTGCCGCCTCCGCTGGATGAACTATCTCCGCCCCTCTGTCAAGCGCGGCCAGATCGCCCCCGATGAAGAAGATCTCATCCTTCGCCTCCATCGCCTTCTGGGCAATCGGTGGTCTTTGATAGCTGGGAGGATTCCAGGCCGTACGGACAATGAGATAAAGAACTACTGGAACACACACCTGAGCAAGAAGCTGATAAGCCAAGGCATAGATCCCAGAACCCACAAGCCTCTCAATTCAGATCATCACTCTGCTGCTGATGATGTTGACGTGGACAACACAAACAAATCAACTGCTGTTGCTTCTTCTTCCAAAGCCAATGATCGGTTCTCAAACCCTAACCCTAGTCCCCCTTCTGATCGTCTTGTCCATAAAGAAGGGGATCCAAATAACAGCCGTAATGATGGAAACATCGCAATTGCTGATCATGATCTGGGCACTATAGTCAATGGCTTTGCAAATATGATCACGTCCATCAACAATCCCGATGCTTCTTCTTCGGCCGCGGCAACGGGTACTTTGAGTTTGAGGAGCAACAACAGCCACGGTGGAGTACTACTTGGGGGAGGAGGAAATGAAGAGGACGACGTCTTCTCTTCGTTTCTGAATTCGTTGATCAATGAGGATCCATTTCCTGGACAACACCAATTGCAACAAGTACTGCAGAATGGGAATGTTAGTGCACACGCAGCTGCTGCTCGTTCCGAGAACCTTCCTTTGATTACTATGACTAGTACTACGGCGCCATCAACATTTGGCTGGGAGTCTGCTGTGCTCATGTCTTCTGCTTTCATCCAAAATGATCACCAGAGCGTTAATGATCAAACGGAGTAG

Protein Analysis

365

Amino Acids

39.07

Weight (kDa)

8.31

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 50 - 97 8.2e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 53 - 110 1.1e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 108 - 148 1.6e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 108 - 151 2.8e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13540
fragaria_vesca FvH4_3g45450
malus_domestica MD11G1006500.v1.1 MD11G1007500.v1.1
prunus_persica Prupe.6G004700_v2.0.a1
pyrus_communis pycom11g00510
rosa_laevigata RLG00000037022
rosa_multiflora Rmu_co8442277.1_g000001 Rmu_sc0011792.1_g000005
rosa_roxburghii Rroxscaffold_1G00001240
rosa_rugosa Rorug05G0483600
rosa_samantha Rh5AG533000 Rh5BG560300 Rh5CG583000 Rh5DG569600
rosa_wichuraiana Rw5G049990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 852
Acc16I TGCGCA 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 1003
AccB7I CCANNNNNTGG 1 cut(s) 896
AccII CGCG 2 cut(s) 309, 767
AclWI GGATC 5 cut(s) 467, 638, 651, 881, 894
AcoI YGGCCR 2 cut(s) 310, 762
AcsI RAATTY 1 cut(s) 868
AcyI GRCGYC 3 cut(s) 119, 849, 1004
AfaI GTAC 5 cut(s) 406, 778, 816, 921, 997
AfiI CCNNNNNNNGG 2 cut(s) 585, 896
AgsI TTSAA 1 cut(s) 145
AhlI ACTAGT 1 cut(s) 992
AjiI CACGTC 2 cut(s) 532, 732
AjnI CCWGG 2 cut(s) 397, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 5 cut(s) 178, 264, 386, 454, 953
AluI AGCT 5 cut(s) 178, 264, 386, 454, 953
Alw21I GWGCWC 2 cut(s) 946, 1038
Alw44I GTGCAC 1 cut(s) 942
AlwI GGATC 5 cut(s) 467, 638, 651, 881, 894
AlwNI CAGNNNCTG 1 cut(s) 956
AoxI GGCC 4 cut(s) 152, 310, 400, 762
ApaLI GTGCAC 1 cut(s) 942
ApoI RAATTY 1 cut(s) 868
AspLEI GCGC 3 cut(s) 131, 309, 1006
AspS9I GGNCC 3 cut(s) 111, 152, 222
AsuHPI GGTGA 1 cut(s) 1058
AvaII GGWCC 2 cut(s) 111, 222
BaeGI GKGCMC 2 cut(s) 701, 946
BamHI GGATCC 2 cut(s) 643, 886
BanI GGYRCC 1 cut(s) 1003
BbsI GAAGAC 3 cut(s) 9, 844, 1035
Bbv12I GWGCWC 2 cut(s) 946, 1038
BccI CCATC 5 cut(s) 19, 360, 659, 743, 1015
BceAI ACGGC 4 cut(s) 106, 387, 642, 1017
BciT130I CCWGG 2 cut(s) 399, 897
BclI TGATCA 5 cut(s) 685, 726, 876, 1063, 1081
BcuI ACTAGT 1 cut(s) 992
BfaI CTAG 2 cut(s) 606, 993
BfmI CTRYAG 2 cut(s) 702, 923
BfoI RGCGCY 1 cut(s) 1007
BglI GCCNNNNNGGC 1 cut(s) 363
BglII AGATCT 1 cut(s) 334
BmcAI AGTACT 3 cut(s) 816, 921, 997
Bme1390I CCNGG 2 cut(s) 399, 897
Bme18I GGWCC 2 cut(s) 111, 222
BmgBI CACGTC 2 cut(s) 532, 732
BmgT120I GGNCC 3 cut(s) 111, 152, 222
BmiI GGNNCC 6 cut(s) 8, 112, 224, 645, 888, 1005
BmrFI CCNGG 2 cut(s) 399, 897
BmsI GCATC 2 cut(s) 42, 739
BpiI GAAGAC 3 cut(s) 9, 844, 1035
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 690
BsaHI GRCGYC 3 cut(s) 119, 849, 1004
BsaJI CCNNGG 4 cut(s) 155, 463, 765, 805
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 2 cut(s) 585, 896
Bse1I ACTGG 1 cut(s) 434
Bse8I GATNNNNATC 1 cut(s) 690
BseBI CCWGG 2 cut(s) 399, 897
BseDI CCNNGG 4 cut(s) 155, 463, 765, 805
BseGI GGATG 3 cut(s) 285, 339, 1053
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 2 cut(s) 585, 896
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 2 cut(s) 805, 843
BseSI GKGCMC 2 cut(s) 701, 946
BseX3I CGGCCG 1 cut(s) 762
BseYI CCCAGC 4 cut(s) 95, 239, 386, 1020
Bsh1236I CGCG 2 cut(s) 309, 767
Bsh1285I CGRYCG 1 cut(s) 765
BshFI GGCC 4 cut(s) 154, 312, 402, 764
BshNI GGYRCC 1 cut(s) 1003
BsiEI CGRYCG 1 cut(s) 765
BsiHKAI GWGCWC 2 cut(s) 946, 1038
BsiWI CGTACG 1 cut(s) 404
BslFI GGGAC 3 cut(s) 124, 225, 594
BslI CCNNNNNNNGG 2 cut(s) 585, 896
BsmFI GGGAC 3 cut(s) 124, 225, 594
BsnI GGCC 4 cut(s) 154, 312, 402, 764
Bsp1286I GDGCHC 3 cut(s) 701, 946, 1038
BspANI GGCC 4 cut(s) 154, 312, 402, 764
BspCNI CTCAG 1 cut(s) 435
BspFNI CGCG 2 cut(s) 309, 767
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 6 cut(s) 8, 112, 224, 645, 888, 1005
BspMAI CTGCAG 1 cut(s) 927
BspPI GGATC 5 cut(s) 467, 638, 651, 881, 894
BspT107I GGYRCC 1 cut(s) 1003
BsrI ACTGG 1 cut(s) 434
BssECI CCNNGG 4 cut(s) 155, 463, 765, 805
BssNI GRCGYC 3 cut(s) 119, 849, 1004
BssT1I CCWWGG 2 cut(s) 155, 463
Bst2UI CCWGG 2 cut(s) 399, 897
Bst4CI ACNGT 2 cut(s) 216, 809
Bst6I CTCTTC 4 cut(s) 140, 162, 834, 862
BstACI GRCGYC 3 cut(s) 119, 849, 1004
BstAPI GCANNNNNTGC 2 cut(s) 44, 956
BstC8I GCNNGC 2 cut(s) 183, 237
BstDEI CTNAG 2 cut(s) 48, 443
BstDSI CCRYGG 2 cut(s) 765, 805
BstF5I GGATG 3 cut(s) 285, 339, 1053
BstFNI CGCG 2 cut(s) 309, 767
BstH2I RGCGCY 1 cut(s) 1007
BstHHI GCGC 3 cut(s) 131, 309, 1006
BstMCI CGRYCG 1 cut(s) 765
BstNI CCWGG 2 cut(s) 399, 897
BstSCI CCNGG 2 cut(s) 397, 895
BstSFI CTRYAG 2 cut(s) 702, 923
BstSLI GKGCMC 2 cut(s) 701, 946
BstUI CGCG 2 cut(s) 309, 767
BstV2I GAAGAC 3 cut(s) 9, 844, 1035
BstX2I RGATCY 4 cut(s) 334, 472, 643, 886
BstYI RGATCY 4 cut(s) 334, 472, 643, 886
BstZI CGGCCG 1 cut(s) 762
BsuRI GGCC 4 cut(s) 154, 312, 402, 764
BtgI CCRYGG 2 cut(s) 765, 805
BtgZI GCGATG 2 cut(s) 338, 658
BtrI CACGTC 2 cut(s) 532, 732
BtsCI GGATG 3 cut(s) 285, 339, 1053
Cac8I GCNNGC 2 cut(s) 183, 237
CaiI CAGNNNCTG 1 cut(s) 956
CciI TCATGA 1 cut(s) 688
CfoI GCGC 3 cut(s) 131, 309, 1006
Cfr13I GGNCC 3 cut(s) 111, 152, 222
Cfr42I CCGCGG 1 cut(s) 768
CseI GACGC 2 cut(s) 60, 127
Csp6I GTAC 5 cut(s) 405, 777, 815, 920, 996
CviAII CATG 2 cut(s) 689, 1039
CviQI GTAC 5 cut(s) 405, 777, 815, 920, 996
DdeI CTNAG 2 cut(s) 48, 443
DinI GGCGCC 1 cut(s) 1005
EaeI YGGCCR 2 cut(s) 310, 762
EagI CGGCCG 1 cut(s) 762
Eam1104I CTCTTC 4 cut(s) 140, 162, 834, 862
EarI CTCTTC 4 cut(s) 140, 162, 834, 862
EciI GGCGGA 2 cut(s) 235, 281
EclXI CGGCCG 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 155, 463
Eco47I GGWCC 2 cut(s) 111, 222
Eco52I CGGCCG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 868
EcoRII CCWGG 2 cut(s) 397, 895
EcoT14I CCWWGG 2 cut(s) 155, 463
EgeI GGCGCC 1 cut(s) 1005
EheI GGCGCC 1 cut(s) 1005
ErhI CCWWGG 2 cut(s) 155, 463
FaeI CATG 2 cut(s) 692, 1042
FaiI YATR 7 cut(s) 470, 633, 690, 704, 725, 989, 1040
FaqI GGGAC 3 cut(s) 124, 225, 594
FatI CATG 2 cut(s) 688, 1038
FauI CCCGC 1 cut(s) 228
FbaI TGATCA 5 cut(s) 685, 726, 876, 1063, 1081
FokI GGATG 3 cut(s) 292, 326, 1040
FspBI CTAG 2 cut(s) 606, 993
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 3 cut(s) 130, 308, 1005
GsaI CCCAGC 4 cut(s) 99, 243, 390, 1024
GsuI CTGGAG 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 1007
HaeIII GGCC 4 cut(s) 154, 312, 402, 764
HgaI GACGC 2 cut(s) 60, 127
HhaI GCGC 3 cut(s) 131, 309, 1006
Hin1I GRCGYC 3 cut(s) 119, 849, 1004
Hin1II CATG 2 cut(s) 692, 1042
Hin6I GCGC 3 cut(s) 129, 307, 1004
HinP1I GCGC 3 cut(s) 129, 307, 1004
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 3 cut(s) 160, 394, 1025
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 3 cut(s) 529, 535, 944
Hpy188I TCNGA 4 cut(s) 502, 619, 867, 967
Hpy188III TCNNGA 4 cut(s) 164, 196, 689, 746
Hpy8I GTNNAC 3 cut(s) 529, 535, 944
Hpy99I CGWCG 5 cut(s) 25, 64, 76, 85, 851
HpyAV CCTTC 7 cut(s) 197, 236, 353, 368, 624, 632, 983
HpyCH4III ACNGT 2 cut(s) 216, 809
HpyCH4IV ACGT 3 cut(s) 531, 731, 849
HpyCH4V TGCA 5 cut(s) 55, 719, 913, 925, 944
HpyF3I CTNAG 2 cut(s) 48, 443
HpySE526I ACGT 3 cut(s) 531, 731, 849
Hsp92I GRCGYC 3 cut(s) 119, 849, 1004
Hsp92II CATG 2 cut(s) 692, 1042
HspAI GCGC 3 cut(s) 129, 307, 1004
KasI GGCGCC 1 cut(s) 1003
Ksp22I TGATCA 5 cut(s) 685, 726, 876, 1063, 1081
KspI CCGCGG 1 cut(s) 768
LmnI GCTCC 2 cut(s) 252, 792
LweI GCATC 2 cut(s) 42, 739
MaeI CTAG 2 cut(s) 606, 993
MaeII ACGT 3 cut(s) 531, 731, 849
MfeI CAATTG 2 cut(s) 678, 908
MflI RGATCY 4 cut(s) 334, 472, 643, 886
MhlI GDGCHC 3 cut(s) 701, 946, 1038
MluCI AATT 5 cut(s) 187, 496, 678, 868, 908
Mly113I GGCGCC 1 cut(s) 1004
MlyI GAGTC 2 cut(s) 154, 1034
MseI TTAA 1 cut(s) 1077
MspA1I CMGCKG 4 cut(s) 252, 276, 767, 953
MspR9I CCNGG 2 cut(s) 399, 897
MunI CAATTG 2 cut(s) 678, 908
MvaI CCWGG 2 cut(s) 399, 897
MvnI CGCG 2 cut(s) 309, 767
NarI GGCGCC 1 cut(s) 1004
NlaIII CATG 2 cut(s) 692, 1042
NlaIV GGNNCC 6 cut(s) 8, 112, 224, 645, 888, 1005
NsbI TGCGCA 1 cut(s) 130
PagI TCATGA 1 cut(s) 688
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 68, 80
PfeI GAWTC 1 cut(s) 394
Pfl23II CGTACG 1 cut(s) 404
PflFI GACNNNGTC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 896
PfoI TCCNGGA 1 cut(s) 895
PleI GAGTC 2 cut(s) 154, 1033
PluTI GGCGCC 1 cut(s) 1007
PpsI GAGTC 2 cut(s) 154, 1033
Psp6I CCWGG 2 cut(s) 397, 895
PspFI CCCAGC 4 cut(s) 95, 239, 386, 1020
PspGI CCWGG 2 cut(s) 397, 895
PspLI CGTACG 1 cut(s) 404
PspN4I GGNNCC 6 cut(s) 8, 112, 224, 645, 888, 1005
PspPI GGNCC 3 cut(s) 111, 152, 222
PstI CTGCAG 1 cut(s) 927
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 4 cut(s) 334, 472, 643, 886
PsyI GACNNNGTC 1 cut(s) 848
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 5 cut(s) 406, 778, 816, 921, 997
RsaNI GTAC 5 cut(s) 405, 777, 815, 920, 996
SacII CCGCGG 1 cut(s) 768
SaqAI TTAA 1 cut(s) 1077
Sau96I GGNCC 3 cut(s) 111, 152, 222
ScaI AGTACT 3 cut(s) 816, 921, 997
SchI GAGTC 2 cut(s) 154, 1034
ScrFI CCNGG 2 cut(s) 399, 897
SduI GDGCHC 3 cut(s) 701, 946, 1038
SfaNI GCATC 2 cut(s) 42, 739
SfcI CTRYAG 2 cut(s) 702, 923
SfoI GGCGCC 1 cut(s) 1005
Sfr303I CCGCGG 1 cut(s) 768
SgrBI CCGCGG 1 cut(s) 768
SinI GGWCC 2 cut(s) 111, 222
SpeI ACTAGT 1 cut(s) 992
Sse9I AATT 5 cut(s) 187, 496, 678, 868, 908
SspDI GGCGCC 1 cut(s) 1003
SspMI CTAG 2 cut(s) 606, 993
StyD4I CCNGG 2 cut(s) 397, 895
StyI CCWWGG 2 cut(s) 155, 463
TaaI ACNGT 2 cut(s) 216, 809
TaiI ACGT 3 cut(s) 534, 734, 852
TaqI TCGA 2 cut(s) 23, 83
TasI AATT 5 cut(s) 187, 496, 678, 868, 908
TatI WGTACW 3 cut(s) 814, 919, 995
TauI GCSGC 5 cut(s) 95, 270, 312, 767, 770
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 1 cut(s) 1077
Tru9I TTAA 1 cut(s) 1077
TspDTI ATGAA 4 cut(s) 296, 342, 852, 1042
TspGWI ACGGA 1 cut(s) 422
Tth111I GACNNNGTC 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 896
VneI GTGCAC 1 cut(s) 942
VpaK11BI GGWCC 2 cut(s) 111, 222
XapI RAATTY 1 cut(s) 868
XspI CTAG 2 cut(s) 606, 993
ZraI GACGTC 1 cut(s) 850
ZrmI AGTACT 3 cut(s) 816, 921, 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.