MD11G1015800.v1.1

plastid-lipid-associated protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007819
Physical Location & Seq
Forward (+)
1252488 .. 1255662
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1015800.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGGCGGCAGCAGTAGCAAATCTAACAAACCTGAGAAGCTTCCATTATTGGCGCCAAAAACCCACATTCTCACCATCGCCAACAACTCTTCCCTTCTACAGAAACTCTTCAGCTTCAAGTGCAGGCCGTACAAAGTCGTTGACGAAAGTGTTGACTCAAGAAGAAGTTTCGTTTACGGAGCAAGAGAACTCCCTCGTCGAAGCCCTCATCGGAATCCAAGGCCGAGGCCGCTCTGCTTCTCCTCAGCAACTAACTGATGTTCAAAATGCTGTGAATGTTCTTGAAGGTTTACAAGGCGTTCCTGACCCGACAAGTTCTAGTTTAATTGAAGGGCGCTGGCAATTGATGTTTACTACAAGACCTGGAACTGCCTCTCCAATCCAAAGGACGTTTGTGGGGGTTGACTTCTTTAGCGTATTTCAAGAGGTATATCTTCGGACAAGTGATCCACGCGTATCGAATATCGTCAAATTCTCTGATGCAGTAGGTGAACTAAAAGTAGAGGCAGCAGCATCAATAAAAGATGGGAAACGAATCCTCTTTCGGTTTGACAAAGCAGCATTTTCTCTTAAATTTTTACCGTTTAAGGTTCCATATCCAGTTCCATTCAGACTTCTCGGAGATGAGGCAAAGGGCTGGTTGGATACCACATACTTGTCCGAATCTGGAAACCTTCGTATCTCAAGAGGAAATAAGGGAACTACATTTGTGCTGCAAAAGAAAACCGGACCTAGGCAAAGATTGCTGTCAACAATCTCCACCGGTACAGGAGTTAAAGAGGCAATCGATGAGTTTATCTCGTTAAATCAGAACATGGGTGATCTGGAACTCCAAGACGGAGAGTGGGAAATGATATGGAGTTCACAGGAAGAGACAGATAGTTGGTTAGAGAATGCAGCCAATGGTCTAATGGGAAAGCAAATTGTCAAGAAAGATGGACAAATAAAGTTTGTGGTTGATACCTTACTTGGACTCAAATTCTCCATTACAGGCACATTCGTGAAAACTGGCGGCAGAACATATGATCTTACAATGGATGACGCGGCCATCATCGGCGGTCAGTTTGGGTATCCTGTAGAGCTAGCGAGCAAGTTCGAACTTGAGATACTGTATAGTGATGACAGGATCAGAATTACACGGGGGTTTAGAAAAATTGTTTTCGTGTATTTACGAACAGATGGTGTTGAACAGAACTAA

Protein Analysis

399

Amino Acids

44.3

Weight (kDa)

8.52

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 62 - 238 5.2e-43 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 231
AccII CGCG 2 cut(s) 453, 1043
AciI CCGC 5 cut(s) 5, 229, 1011, 1043, 1056
AclWI GGATC 2 cut(s) 440, 1133
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 3 cut(s) 470, 572, 977
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 52
AfaI GTAC 2 cut(s) 130, 766
AfiI CCNNNNNNNGG 1 cut(s) 732
AflIII ACRYGT 1 cut(s) 451
AgeI ACCGGT 1 cut(s) 761
AgsI TTSAA 6 cut(s) 117, 263, 284, 329, 422, 1187
AjnI CCWGG 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 998
AloI GAACNNNNNNTCC 2 cut(s) 358, 390
AluBI AGCT 3 cut(s) 39, 113, 1081
AluI AGCT 3 cut(s) 39, 113, 1081
Alw26I GTCTC 1 cut(s) 866
AlwI GGATC 2 cut(s) 440, 1133
AoxI GGCC 4 cut(s) 124, 220, 226, 1044
ApeKI GCWGC 6 cut(s) 8, 506, 509, 557, 712, 896
ApoI RAATTY 3 cut(s) 470, 572, 977
AsiGI ACCGGT 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 106
AspA2I CCTAGG 1 cut(s) 731
AspLEI GCGC 2 cut(s) 54, 336
AspS9I GGNCC 1 cut(s) 728
AsuHPI GGTGA 3 cut(s) 63, 500, 830
AsuII TTCGAA 1 cut(s) 1095
AsuNHI GCTAGC 1 cut(s) 1081
AvaII GGWCC 1 cut(s) 728
AvrII CCTAGG 1 cut(s) 731
BaeI ACNNNNGTAYC 4 cut(s) 661, 694, 756, 789
BanI GGYRCC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 6 cut(s) 20, 518, 521, 569, 699, 908
BccI CCATC 5 cut(s) 82, 518, 929, 1055, 1172
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 1 cut(s) 363
BciVI GTATCC 2 cut(s) 637, 1080
BcoDI GTCTC 1 cut(s) 866
BfaI CTAG 3 cut(s) 318, 732, 1082
BfmI CTRYAG 2 cut(s) 97, 1074
BfoI RGCGCY 2 cut(s) 55, 337
BfuI GTATCC 2 cut(s) 637, 1080
BlnI CCTAGG 1 cut(s) 731
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 728
BmiI GGNNCC 2 cut(s) 53, 591
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 469, 521
BmtI GCTAGC 1 cut(s) 1085
BplI GAGNNNNNCTC 2 cut(s) 782, 814
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 1095
BpuEI CTTGAG 3 cut(s) 141, 667, 1121
Bsa29I ATCGAT 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 217, 223, 731
BsaWI WCCGGW 2 cut(s) 725, 761
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 1 cut(s) 732
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 2 cut(s) 599, 1012
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 3 cut(s) 217, 223, 731
BseGI GGATG 1 cut(s) 1042
BseLI CCNNNNNNNGG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 23, 257
BseNI ACTGG 2 cut(s) 599, 1012
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 6 cut(s) 20, 518, 521, 569, 699, 908
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 453, 1043
BshFI GGCC 4 cut(s) 126, 222, 228, 1046
BshNI GGYRCC 1 cut(s) 51
BshTI ACCGGT 1 cut(s) 761
BshVI ATCGAT 1 cut(s) 786
BsiSI CCGG 2 cut(s) 726, 762
BslI CCNNNNNNNGG 1 cut(s) 732
BsmAI GTCTC 1 cut(s) 866
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 4 cut(s) 126, 222, 228, 1046
Bsp119I TTCGAA 1 cut(s) 1095
Bsp143I GATC 4 cut(s) 445, 820, 1024, 1125
BspACI CCGC 5 cut(s) 5, 229, 1011, 1043, 1056
BspANI GGCC 4 cut(s) 126, 222, 228, 1046
BspCNI CTCAG 2 cut(s) 24, 256
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 2 cut(s) 453, 1043
BspLI GGNNCC 2 cut(s) 53, 591
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 2 cut(s) 440, 1133
BspT104I TTCGAA 1 cut(s) 1095
BspT107I GGYRCC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 761
BsrI ACTGG 2 cut(s) 599, 1012
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 217, 223, 731
BssMI GATC 4 cut(s) 445, 820, 1024, 1125
BssNI GRCGYC 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 217, 731
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 582, 1110
Bst6I CTCTTC 3 cut(s) 93, 112, 864
BstACI GRCGYC 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 742
BstBI TTCGAA 1 cut(s) 1095
BstC8I GCNNGC 4 cut(s) 124, 338, 1083, 1087
BstDEI CTNAG 2 cut(s) 32, 243
BstF5I GGATG 1 cut(s) 1042
BstFNI CGCG 2 cut(s) 453, 1043
BstH2I RGCGCY 2 cut(s) 55, 337
BstHHI GCGC 2 cut(s) 54, 336
BstKTI GATC 4 cut(s) 448, 823, 1027, 1128
BstMAI GTCTC 1 cut(s) 866
BstMBI GATC 4 cut(s) 445, 820, 1024, 1125
BstMWI GCNNNNNNNGC 4 cut(s) 14, 119, 228, 742
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 2 cut(s) 97, 1074
BstUI CGCG 2 cut(s) 453, 1043
BstV1I GCAGC 6 cut(s) 20, 518, 521, 569, 699, 908
Bsu15I ATCGAT 1 cut(s) 786
BsuI GTATCC 2 cut(s) 637, 1080
BsuRI GGCC 4 cut(s) 126, 222, 228, 1046
BsuTUI ATCGAT 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 1042
Cac8I GCNNGC 4 cut(s) 124, 338, 1083, 1087
CfoI GCGC 2 cut(s) 54, 336
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 1 cut(s) 728
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 1 cut(s) 1049
Csp6I GTAC 2 cut(s) 129, 765
CspAI ACCGGT 1 cut(s) 761
CviAII CATG 1 cut(s) 814
CviQI GTAC 2 cut(s) 129, 765
DdeI CTNAG 2 cut(s) 32, 243
DinI GGCGCC 1 cut(s) 53
DpnI GATC 4 cut(s) 447, 822, 1026, 1127
DpnII GATC 4 cut(s) 445, 820, 1024, 1125
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 3 cut(s) 93, 112, 864
EarI CTCTTC 3 cut(s) 93, 112, 864
Eco130I CCWWGG 2 cut(s) 217, 731
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 217, 731
EgeI GGCGCC 1 cut(s) 53
EheI GGCGCC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 217, 731
FaeI CATG 1 cut(s) 817
FaiI YATR 8 cut(s) 430, 595, 652, 815, 856, 1021, 1023, 1113
FatI CATG 1 cut(s) 813
FauNDI CATATG 1 cut(s) 1021
FokI GGATG 1 cut(s) 1049
FspBI CTAG 3 cut(s) 318, 732, 1082
GlaI GCGC 2 cut(s) 53, 335
HaeII RGCGCY 2 cut(s) 55, 337
HaeIII GGCC 4 cut(s) 126, 222, 228, 1046
HapII CCGG 2 cut(s) 726, 762
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 2 cut(s) 54, 336
Hin1I GRCGYC 1 cut(s) 52
Hin1II CATG 1 cut(s) 817
Hin6I GCGC 2 cut(s) 52, 334
HinP1I GCGC 2 cut(s) 52, 334
HincII GTYRAC 4 cut(s) 141, 153, 403, 750
HindII GTYRAC 4 cut(s) 141, 153, 403, 750
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 5 cut(s) 154, 213, 534, 662, 972
HpaII CCGG 2 cut(s) 726, 762
HphI GGTGA 3 cut(s) 63, 500, 830
Hpy166II GTNNAC 9 cut(s) 141, 153, 174, 290, 351, 403, 491, 750, 863
Hpy188I TCNGA 8 cut(s) 212, 438, 478, 611, 620, 661, 810, 1130
Hpy188III TCNNGA 9 cut(s) 158, 281, 302, 422, 666, 684, 824, 928, 1000
Hpy8I GTNNAC 9 cut(s) 141, 153, 174, 290, 351, 403, 491, 750, 863
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 4 cut(s) 103, 278, 323, 683
HpyCH4III ACNGT 2 cut(s) 582, 1110
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 4 cut(s) 122, 482, 715, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 119, 228, 742
HpyF3I CTNAG 2 cut(s) 32, 243
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 52
Hsp92II CATG 1 cut(s) 817
HspAI GCGC 2 cut(s) 52, 334
KasI GGCGCC 1 cut(s) 51
Kzo9I GATC 4 cut(s) 445, 820, 1024, 1125
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 6 cut(s) 20, 518, 521, 569, 699, 908
LweI GCATC 2 cut(s) 469, 521
MaeI CTAG 3 cut(s) 318, 732, 1082
MaeII ACGT 1 cut(s) 389
MalI GATC 4 cut(s) 447, 822, 1026, 1127
MbiI CCGCTC 1 cut(s) 231
MboI GATC 4 cut(s) 445, 820, 1024, 1125
MboII GAAGA 5 cut(s) 80, 99, 173, 425, 881
MfeI CAATTG 1 cut(s) 341
MluCI AATT 8 cut(s) 324, 341, 470, 572, 921, 977, 1131, 1152
MluI ACGCGT 1 cut(s) 451
Mly113I GGCGCC 1 cut(s) 52
MlyI GAGTC 2 cut(s) 148, 966
MmeI TCCRAC 1 cut(s) 621
MroXI GAANNNNTTC 1 cut(s) 106
MseI TTAA 5 cut(s) 323, 570, 585, 774, 803
MslI CAYNNNNRTG 1 cut(s) 998
MspI CCGG 2 cut(s) 726, 762
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 341
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 2 cut(s) 453, 1043
MwoI GCNNNNNNNGC 4 cut(s) 14, 119, 228, 742
NarI GGCGCC 1 cut(s) 52
NdeI CATATG 1 cut(s) 1021
NdeII GATC 4 cut(s) 445, 820, 1024, 1125
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 1 cut(s) 817
NlaIV GGNNCC 2 cut(s) 53, 591
NmeAIII GCCGAG 1 cut(s) 248
NspV TTCGAA 1 cut(s) 1095
OliI CACNNNNGTG 1 cut(s) 998
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 106
PfeI GAWTC 3 cut(s) 213, 534, 662
PinAI ACCGGT 1 cut(s) 761
PleI GAGTC 2 cut(s) 148, 966
PluTI GGCGCC 1 cut(s) 55
PpsI GAGTC 2 cut(s) 148, 966
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 53, 591
PspPI GGNCC 1 cut(s) 728
PsrI GAACNNNNNNTAC 2 cut(s) 1089, 1121
RsaI GTAC 2 cut(s) 130, 766
RsaNI GTAC 2 cut(s) 129, 765
RseI CAYNNNNRTG 1 cut(s) 998
SaqAI TTAA 5 cut(s) 323, 570, 585, 774, 803
Sau3AI GATC 4 cut(s) 445, 820, 1024, 1125
Sau96I GGNCC 1 cut(s) 728
SchI GAGTC 2 cut(s) 148, 966
ScrFI CCNGG 1 cut(s) 363
SfaNI GCATC 2 cut(s) 469, 521
SfcI CTRYAG 2 cut(s) 97, 1074
SfoI GGCGCC 1 cut(s) 53
SfuI TTCGAA 1 cut(s) 1095
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 3 cut(s) 156, 682, 1100
SmoI CTYRAG 3 cut(s) 156, 682, 1100
Sse9I AATT 8 cut(s) 324, 341, 470, 572, 921, 977, 1131, 1152
SsiI CCGC 5 cut(s) 5, 229, 1011, 1043, 1056
SspDI GGCGCC 1 cut(s) 51
SspMI CTAG 3 cut(s) 318, 732, 1082
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 2 cut(s) 217, 731
TaaI ACNGT 2 cut(s) 582, 1110
TaiI ACGT 1 cut(s) 392
TaqI TCGA 4 cut(s) 198, 458, 786, 1095
TasI AATT 8 cut(s) 324, 341, 470, 572, 921, 977, 1131, 1152
TauI GCSGC 4 cut(s) 8, 231, 1014, 1046
TfiI GAWTC 3 cut(s) 213, 534, 662
Tru1I TTAA 5 cut(s) 323, 570, 585, 774, 803
Tru9I TTAA 5 cut(s) 323, 570, 585, 774, 803
TseI GCWGC 6 cut(s) 8, 506, 509, 557, 712, 896
TspGWI ACGGA 2 cut(s) 191, 852
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 3 cut(s) 470, 572, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XmaJI CCTAGG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 106
XspI CTAG 3 cut(s) 318, 732, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.