MD11G1027100.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007439
Physical Location & Seq
Reverse (-)
2379936 .. 2385688
5753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1027100.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGGAAAAATACTTGGTCAGTCTCAAGCCCTCGTCTCAAAATCCCAGCAAACCTATCTCACGGTGCAGATGGAGTCGGAGCATGATCGAACTGAACGGAAGATTCGAGCCCAATTACCGCCATTCTGTCTCCGGTTTACTCATGGAATCATATTCGGAGCTTAAGAAATTTCCGCATTTGTACCACGTCAATGGCGGACCTTGTCAAACCCATTTGAATTGGATCGACAATGAAGCGTTTCTTTATCGCCAAGCGCCAATCACAAAGCAAGGAATCTCTGCACTGGATTTTGACCAAAAGGGAATATACCTGGCATCTGTGACAAAATCAGGGTGCTTAACAGTGCATGATTTCGAAACTCTTTATTGCCAGAGTAATGAACCTTTGCACCGCTTGAAGGAAGATGAATCAAAACATGTGGTGCACCTTTCTTTCGATCAACAACTAGACTTTGTTCAGTGGAATCTTGCTAACCAAGATGAGGTTGTTTGCACATCTACAAAAAGGAATGAAGTACTTATATTTGATATTAGTTATATCTCATCTAAACCATCCCAGGTGTTAAGGACAAGACATAGTGTTTCCGTCTTTGGGTCTAATATTCATAAGGGCCTTACTGATACTGCTTCTACTTCACTTGATGATTCCAGAATACTGGCTTCTGATACACATGGTGCAATCAACGTATGGGACAGAAGAAAGAGCACATTTCCATGTCTTGAACTCACTACAGATTCCCGTGGAACCCTTAATAGTATCCAATTGAATGTGGAAAATCAGATCGTTTTTGGAGCTGGAAAGAATGGATTTATTTATATATGGGATCTTCGTGGTGGAAGAACATCTTCTACTCTTCAGAGTAATAAAGAAATACGTCATCCACCTCTAACATCATTGAAGTTATCATCAATGTTAGAGAAAATTGGGCCTCTGAAGGCACAATCAGACATTGTTTCTAAGGAGATACAATCCATAAATCTCGATCCATCCTGCCCGCATCAGTTGGCATTCCACCTTGATGATGGTTGGTCAGGTGTTTTGGATGTTTATAATTTTCAAGTTACACATATTCATTGTCCTCCGCCTGCTTGGTTAAATGATTCTGGTGTGTCAGCTGATCTGTTATATTTGAGAAAACCTTCATGGCTGCCAATAAATTCGATCTATGTGGTTGGATCATCAACTGACAGTGGGATTTATATTTTGGATTTCTATCCCAATTCAAGCTCTCCTAGCCATGTGGACTACATAGACGATGCACAAAATCTTTCTGGGGTAAAGAATCAAGATAAGAAAAACAGGTTTCTTCCATTGTCAGAAAGAGTTACGGCATGTGCAGTTCATCCTCTCAACAGTACCATTATTGCTGGAACAAGGAATTCATCTTTGCTCATGATTTCGCAAAGTCACAAGTCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

474

Amino Acids

53.08

Weight (kDa)

7.02

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 89 - 300 9.1e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
AciI CCGC 6 cut(s) 118, 173, 195, 391, 995, 1082
AclWI GGATC 4 cut(s) 230, 831, 977, 1183
AcsI RAATTY 3 cut(s) 167, 1156, 1378
AcuI CTGAAG 2 cut(s) 839, 953
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 3 cut(s) 182, 516, 1357
AfiI CCNNNNNNNGG 3 cut(s) 397, 481, 591
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 7 cut(s) 217, 397, 722, 766, 898, 1058, 1224
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 2 cut(s) 309, 555
AluBI AGCT 4 cut(s) 160, 794, 1115, 1227
AluI AGCT 4 cut(s) 160, 794, 1115, 1227
Alw21I GWGCWC 2 cut(s) 426, 707
Alw26I GTCTC 3 cut(s) 26, 39, 133
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 4 cut(s) 230, 831, 977, 1183
AoxI GGCC 2 cut(s) 610, 926
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 1147
ApoI RAATTY 3 cut(s) 167, 1156, 1378
Asp700I GAANNNNTTC 3 cut(s) 237, 844, 1138
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 3 cut(s) 197, 610, 926
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 426
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 2 cut(s) 426, 707
BbvI GCAGC 1 cut(s) 1134
BccI CCATC 4 cut(s) 63, 559, 994, 1016
BceAI ACGGC 1 cut(s) 1344
BciT130I CCWGG 2 cut(s) 311, 557
BciVI GTATCC 1 cut(s) 767
BcoDI GTCTC 3 cut(s) 26, 39, 133
BfaI CTAG 2 cut(s) 446, 1233
BfmI CTRYAG 1 cut(s) 729
BfoI RGCGCY 1 cut(s) 257
BfrI CTTAAG 1 cut(s) 161
BfuI GTATCC 1 cut(s) 767
BisI GCNGC 1 cut(s) 1148
BlsI GCNGC 1 cut(s) 1149
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 2 cut(s) 311, 557
Bme18I GGWCC 1 cut(s) 197
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 3 cut(s) 197, 610, 926
BmiI GGNNCC 1 cut(s) 745
BmrFI CCNGG 2 cut(s) 311, 557
BmsI GCATC 3 cut(s) 323, 1006, 1246
Bpu14I TTCGAA 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 2 cut(s) 555, 739
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 3 cut(s) 397, 481, 591
Bse1I ACTGG 2 cut(s) 288, 660
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 311, 557
BseDI CCNNGG 2 cut(s) 555, 739
BseGI GGATG 5 cut(s) 551, 877, 986, 1048, 1342
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 3 cut(s) 397, 481, 591
BseNI ACTGG 2 cut(s) 288, 660
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 1 cut(s) 1134
BseYI CCCAGC 1 cut(s) 44
BsgI GTGCAG 3 cut(s) 85, 264, 1356
BshFI GGCC 2 cut(s) 612, 928
BsiHKAI GWGCWC 2 cut(s) 426, 707
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 3 cut(s) 397, 481, 591
BsmAI GTCTC 3 cut(s) 26, 39, 133
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 612, 928
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 3 cut(s) 111, 426, 707
Bsp143I GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
BspACI CCGC 6 cut(s) 118, 173, 195, 391, 995, 1082
BspANI GGCC 2 cut(s) 612, 928
BspHI TCATGA 1 cut(s) 1392
BspLI GGNNCC 1 cut(s) 745
BspPI GGATC 4 cut(s) 230, 831, 977, 1183
BspT104I TTCGAA 1 cut(s) 354
BspTI CTTAAG 1 cut(s) 161
BsrI ACTGG 2 cut(s) 288, 660
BssECI CCNNGG 2 cut(s) 555, 739
BssMI GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
Bst2UI CCWGG 2 cut(s) 311, 557
Bst4CI ACNGT 4 cut(s) 63, 343, 1190, 1355
Bst6I CTCTTC 1 cut(s) 858
BstAFI CTTAAG 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 995, 1086
BstDEI CTNAG 1 cut(s) 957
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 5 cut(s) 551, 877, 986, 1048, 1342
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 9 cut(s) 87, 225, 439, 783, 826, 985, 1120, 1164, 1178
BstMAI GTCTC 3 cut(s) 26, 39, 133
BstMBI GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
BstMWI GCNNNNNNNGC 1 cut(s) 1233
BstNI CCWGG 2 cut(s) 311, 557
BstNSI RCATGY 2 cut(s) 419, 1335
BstSCI CCNGG 2 cut(s) 309, 555
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 1134
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 2 cut(s) 191, 655
BstYI RGATCY 1 cut(s) 823
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 2 cut(s) 612, 928
BtgI CCRYGG 1 cut(s) 739
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 5 cut(s) 551, 877, 986, 1048, 1342
BtsIMutI CAGTG 4 cut(s) 281, 348, 464, 1195
Cac8I GCNNGC 2 cut(s) 995, 1086
CciI TCATGA 1 cut(s) 1392
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 3 cut(s) 197, 610, 926
Csp6I GTAC 3 cut(s) 181, 515, 1356
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviQI GTAC 3 cut(s) 181, 515, 1356
DdeI CTNAG 1 cut(s) 957
DpnI GATC 9 cut(s) 86, 224, 438, 782, 825, 984, 1119, 1163, 1177
DpnII GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
DraIII CACNNNGTG 1 cut(s) 674
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
EciI GGCGGA 2 cut(s) 210, 1071
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 839, 953
EcoO109I RGGNCCY 1 cut(s) 610
EcoRI GAATTC 1 cut(s) 1378
EcoRII CCWGG 2 cut(s) 309, 555
EcoT38I GRGCYC 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 39
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 829, 861
FaqI GGGAC 1 cut(s) 704
FauI CCCGC 1 cut(s) 1002
Fnu4HI GCNGC 1 cut(s) 1148
FokI GGATG 5 cut(s) 538, 864, 973, 1055, 1329
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 1148
FspBI CTAG 2 cut(s) 446, 1233
GlaI GCGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 1148
GsaI CCCAGC 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 257
HaeIII GGCC 2 cut(s) 612, 928
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 256
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HpaII CCGG 1 cut(s) 132
Hpy166II GTNNAC 3 cut(s) 137, 424, 1243
Hpy188I TCNGA 8 cut(s) 78, 157, 664, 780, 858, 933, 946, 1318
Hpy188III TCNNGA 5 cut(s) 648, 719, 980, 1286, 1393
Hpy8I GTNNAC 3 cut(s) 137, 424, 1243
HpyAV CCTTC 3 cut(s) 391, 928, 1149
HpyCH4III ACNGT 4 cut(s) 63, 343, 1190, 1355
HpyCH4IV ACGT 3 cut(s) 186, 684, 874
HpyCH4V TGCA 9 cut(s) 66, 281, 346, 388, 424, 492, 677, 1259, 1337
HpyF10VI GCNNNNNNNGC 1 cut(s) 1233
HpyF3I CTNAG 1 cut(s) 957
HpySE526I ACGT 3 cut(s) 186, 684, 874
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
LmnI GCTCC 3 cut(s) 78, 157, 791
Lsp1109I GCAGC 1 cut(s) 1134
LweI GCATC 3 cut(s) 323, 1006, 1246
MaeI CTAG 2 cut(s) 446, 1233
MaeII ACGT 3 cut(s) 186, 684, 874
MaeIII GTNAC 4 cut(s) 319, 1060, 1324, 1406
MalI GATC 9 cut(s) 86, 224, 438, 782, 825, 984, 1119, 1163, 1177
MboI GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
MboII GAAGA 8 cut(s) 111, 413, 708, 818, 837, 845, 849, 1298
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 3 cut(s) 111, 426, 707
MluCI AATT 9 cut(s) 112, 167, 217, 761, 921, 1051, 1156, 1219, 1378
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 2 cut(s) 56, 1153
MnlI CCTC 6 cut(s) 40, 475, 894, 939, 1089, 1356
MroXI GAANNNNTTC 3 cut(s) 237, 844, 1138
MseI TTAA 6 cut(s) 162, 338, 563, 750, 1094, 1420
MslI CAYNNNNRTG 3 cut(s) 189, 712, 1017
MspA1I CMGCKG 1 cut(s) 1115
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 311, 557
MunI CAATTG 1 cut(s) 761
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 2 cut(s) 311, 557
MwoI GCNNNNNNNGC 1 cut(s) 1233
NdeII GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
NlaIV GGNNCC 1 cut(s) 745
NmuCI GTSAC 2 cut(s) 319, 1406
NspI RCATGY 2 cut(s) 419, 1335
NspV TTCGAA 1 cut(s) 354
PagI TCATGA 1 cut(s) 1392
PciI ACATGT 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 1007
PdmI GAANNNNTTC 3 cut(s) 237, 844, 1138
PfeI GAWTC 9 cut(s) 102, 146, 273, 407, 463, 644, 734, 1100, 1282
PflFI GACNNNGTC 1 cut(s) 201
PkrI GCNGC 1 cut(s) 1149
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 415
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 309, 555
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 309, 555
PspN4I GGNNCC 1 cut(s) 745
PspPI GGNCC 3 cut(s) 197, 610, 926
PsrI GAACNNNNNNTAC 2 cut(s) 832, 864
PsuI RGATCY 1 cut(s) 823
PsyI GACNNNGTC 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 3 cut(s) 182, 516, 1357
RsaNI GTAC 3 cut(s) 181, 515, 1356
RseI CAYNNNNRTG 3 cut(s) 189, 712, 1017
SaqAI TTAA 6 cut(s) 162, 338, 563, 750, 1094, 1420
SatI GCNGC 1 cut(s) 1148
Sau3AI GATC 9 cut(s) 84, 222, 436, 780, 823, 982, 1117, 1161, 1175
Sau96I GGNCC 3 cut(s) 197, 610, 926
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 311, 557
SduI GDGCHC 3 cut(s) 111, 426, 707
SfaNI GCATC 3 cut(s) 323, 1006, 1246
SfcI CTRYAG 1 cut(s) 729
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 3 cut(s) 189, 712, 1017
SmlI CTYRAG 2 cut(s) 23, 161
SmoI CTYRAG 2 cut(s) 23, 161
Sse9I AATT 9 cut(s) 112, 167, 217, 761, 921, 1051, 1156, 1219, 1378
SsiI CCGC 6 cut(s) 118, 173, 195, 391, 995, 1082
SspI AATATT 1 cut(s) 601
SspMI CTAG 2 cut(s) 446, 1233
StyD4I CCNGG 2 cut(s) 309, 555
TaaI ACNGT 4 cut(s) 63, 343, 1190, 1355
TaiI ACGT 3 cut(s) 189, 687, 877
TaqI TCGA 7 cut(s) 87, 105, 225, 354, 435, 981, 1160
TasI AATT 9 cut(s) 112, 167, 217, 761, 921, 1051, 1156, 1219, 1378
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 9 cut(s) 102, 146, 273, 407, 463, 644, 734, 1100, 1282
Tru1I TTAA 6 cut(s) 162, 338, 563, 750, 1094, 1420
Tru9I TTAA 6 cut(s) 162, 338, 563, 750, 1094, 1420
TscAI CASTG 4 cut(s) 288, 348, 464, 1195
TseFI GTSAC 2 cut(s) 319, 1406
TseI GCWGC 1 cut(s) 1147
Tsp45I GTSAC 2 cut(s) 319, 1406
TspDTI ATGAA 9 cut(s) 246, 393, 420, 525, 593, 1061, 1131, 1331, 1371
TspGWI ACGGA 2 cut(s) 111, 574
TspRI CASTG 4 cut(s) 288, 348, 464, 1195
Tth111I GACNNNGTC 1 cut(s) 201
Vha464I CTTAAG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 3 cut(s) 167, 1156, 1378
XceI RCATGY 2 cut(s) 419, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XmnI GAANNNNTTC 3 cut(s) 237, 844, 1138
XspI CTAG 2 cut(s) 446, 1233
ZrmI AGTACT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.