Rh5DG543600

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007439
Physical Location & Seq
Forward (+)
83624798 .. 83630807
6010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG543600.1

Sequence Viewer

Length: 960 bp
ATGGACAAATTCTTACTCCCTCTCAAACCCTCACCTCAACTTTCCACCAAACCCATTCGAAGGCGAAGATGGCATCGGAGCCTCATCGAATTGAACGGAAAGCTCGAACCCAAGTACCGGCATTCCGTCTCTGGTTTACTCATGCAATCCTATTCCGAGCTTAGGGCGTTTCGGCATTTGTACCACATCGACGGACACCCGTGTCAAACCCATGTGAATCGGGTGGTGATTAATGGCGCGTACATACCCCCAAGTATAAAGGAAGGAGTTTCTGCCCTGGATTTTGACAACAAGGGGATATACCTGGCATCTGTGACAAGATCGGGGTGCTTAATGGTGCATGATTTTGAAACTCTTTATTGCCAGAGTAATGAACCATTGCTACGCTTGGAAGAAGATGAAGCAAAACATGTGCTGCACCTTTCTTTACATCAACAACTAGACTTTGTCCGGTGGAATTGTGCCAACCAAGATGAGGTTGTTTGCACTTCTATGAAAAGGAATGAAGTACTTATATTTGACATTAGTTATATATCTTCTACACCATCCGAAGTTTTAAGAACAAAACCCCGTCCTGCTATCTTTGGGTCTAATATTCAGAAAGGTCTTTCTGATATTGCCTTTACCTCTTGTGAAGACTCAAGGGTACTTGCTTCTGATACAGATGGTGTTATTCATATATGGGACAGAAGAACTAGTACTTTTCCTTGTCTTGAACTTACGGCAAATTCCCGTGGCAGCCTTAATAGTATCCAATTAAATGTGGAAAATCAGATCATTTTTGGAGCTGGAAAGCATGGGGTGATCTATGCTTGGGATCTTCGTGGTGGAAGAACGTCTTCTTTCTTCAAGGGTTCTAAAGAGGTATCTCGTCCACCTCTAACCTCACTGAAGTTATCATCAACATTGGAAAAAATTGGGCCTCTAAAGGTAATCACAGAAGTAAATAACTTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

319

Amino Acids

36.08

Weight (kDa)

8.91

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 201
AccII CGCG 1 cut(s) 239
AclWI GGATC 1 cut(s) 825
AcsI RAATTY 2 cut(s) 8, 727
AcuI CTGAAG 1 cut(s) 911
AfaI GTAC 6 cut(s) 116, 182, 242, 510, 648, 700
AfiI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 4 cut(s) 94, 350, 716, 850
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 2 cut(s) 276, 303
AluBI AGCT 3 cut(s) 103, 160, 788
AluI AGCT 3 cut(s) 103, 160, 788
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 1 cut(s) 825
AoxI GGCC 1 cut(s) 920
ApeKI GCWGC 2 cut(s) 415, 738
ApoI RAATTY 2 cut(s) 8, 727
AseI ATTAAT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 838
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 920
AsuHPI GGTGA 3 cut(s) 24, 238, 814
AsuII TTCGAA 1 cut(s) 58
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BarI GAAGNNNNNNTAC 2 cut(s) 682, 714
BbsI GAAGAC 2 cut(s) 642, 831
BbvI GCAGC 2 cut(s) 402, 750
BccI CCATC 3 cut(s) 63, 553, 659
BceAI ACGGC 1 cut(s) 738
BciT130I CCWGG 2 cut(s) 278, 305
BciVI GTATCC 1 cut(s) 761
BcoDI GTCTC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 2 cut(s) 440, 696
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 2 cut(s) 416, 739
BlsI GCNGC 2 cut(s) 417, 740
BmcAI AGTACT 2 cut(s) 510, 700
Bme1390I CCNGG 2 cut(s) 278, 305
BmgT120I GGNCC 1 cut(s) 920
BmiI GGNNCC 1 cut(s) 80
BmrFI CCNGG 2 cut(s) 278, 305
BmsI GCATC 2 cut(s) 82, 317
BpiI GAAGAC 2 cut(s) 642, 831
Bpu10I CCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 58
BpuEI CTTGAG 1 cut(s) 625
BsaJI CCNNGG 2 cut(s) 276, 733
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
Bse118I RCCGGY 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 377
BseBI CCWGG 2 cut(s) 278, 305
BseDI CCNNGG 2 cut(s) 276, 733
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
BseMI GCAATG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 402, 750
BsgI GTGCAG 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 1 cut(s) 922
BsiSI CCGG 2 cut(s) 118, 451
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 1 cut(s) 922
Bsp119I TTCGAA 1 cut(s) 58
Bsp143I GATC 4 cut(s) 320, 774, 804, 817
BspANI GGCC 1 cut(s) 922
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 80
BspPI GGATC 1 cut(s) 825
BspT104I TTCGAA 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 276, 733
BssMI GATC 4 cut(s) 320, 774, 804, 817
Bst2UI CCWGG 2 cut(s) 278, 305
BstBI TTCGAA 1 cut(s) 58
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 1 cut(s) 545
BstFNI CGCG 1 cut(s) 239
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 4 cut(s) 323, 777, 807, 820
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 4 cut(s) 320, 774, 804, 817
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstNI CCWGG 2 cut(s) 278, 305
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 2 cut(s) 276, 303
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 402, 750
BstV2I GAAGAC 2 cut(s) 642, 831
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 1 cut(s) 922
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 1 cut(s) 545
BtsIMutI CAGTG 1 cut(s) 887
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 920
Csp6I GTAC 6 cut(s) 115, 181, 241, 509, 647, 699
CviAII CATG 5 cut(s) 142, 212, 341, 410, 797
CviJI RGCY 6 cut(s) 81, 103, 160, 741, 788, 922
CviKI_1 RGCY 6 cut(s) 81, 103, 160, 741, 788, 922
CviQI GTAC 6 cut(s) 115, 181, 241, 509, 647, 699
DdeI CTNAG 1 cut(s) 161
DpnI GATC 4 cut(s) 322, 776, 806, 819
DpnII GATC 4 cut(s) 320, 774, 804, 817
DrdI GACNNNNNNGTC 1 cut(s) 201
DseDI GACNNNNNNGTC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 911
EcoRII CCWGG 2 cut(s) 276, 303
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 5 cut(s) 145, 215, 344, 413, 800
FalI AAGNNNNNCTT 2 cut(s) 823, 855
FaqI GGGAC 1 cut(s) 698
FatI CATG 5 cut(s) 141, 211, 340, 409, 796
Fnu4HI GCNGC 2 cut(s) 416, 739
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 2 cut(s) 416, 739
FspBI CTAG 2 cut(s) 440, 696
GlaI GCGC 1 cut(s) 238
GluI GCNGC 2 cut(s) 416, 739
HaeIII GGCC 1 cut(s) 922
HapII CCGG 2 cut(s) 118, 451
HhaI GCGC 1 cut(s) 239
Hin1II CATG 5 cut(s) 145, 215, 344, 413, 800
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HinfI GANTC 2 cut(s) 217, 638
HpaII CCGG 2 cut(s) 118, 451
HphI GGTGA 3 cut(s) 24, 238, 814
Hpy166II GTNNAC 2 cut(s) 137, 875
Hpy188I TCNGA 7 cut(s) 78, 157, 550, 600, 613, 658, 774
Hpy188III TCNNGA 1 cut(s) 713
Hpy8I GTNNAC 2 cut(s) 137, 875
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 54, 257
HpyCH4IV ACGT 1 cut(s) 836
HpyCH4V TGCA 5 cut(s) 145, 340, 418, 486, 955
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 836
Hsp92II CATG 5 cut(s) 145, 215, 344, 413, 800
HspAI GCGC 1 cut(s) 237
Kzo9I GATC 4 cut(s) 320, 774, 804, 817
LmnI GCTCC 2 cut(s) 78, 785
Lsp1109I GCAGC 2 cut(s) 402, 750
LweI GCATC 2 cut(s) 82, 317
MaeI CTAG 2 cut(s) 440, 696
MaeII ACGT 1 cut(s) 836
MaeIII GTNAC 1 cut(s) 313
MalI GATC 4 cut(s) 322, 776, 806, 819
MboI GATC 4 cut(s) 320, 774, 804, 817
MflI RGATCY 1 cut(s) 817
MluCI AATT 6 cut(s) 8, 89, 457, 727, 755, 915
MlyI GAGTC 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 838
MseI TTAA 5 cut(s) 231, 332, 557, 744, 758
MslI CAYNNNNRTG 1 cut(s) 491
MspI CCGG 2 cut(s) 118, 451
MspR9I CCNGG 2 cut(s) 278, 305
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 2 cut(s) 278, 305
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 4 cut(s) 320, 774, 804, 817
NlaIII CATG 5 cut(s) 145, 215, 344, 413, 800
NlaIV GGNNCC 1 cut(s) 80
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 1 cut(s) 413
NspV TTCGAA 1 cut(s) 58
PciI ACATGT 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 1 cut(s) 217
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 417, 740
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
PscI ACATGT 1 cut(s) 409
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 276, 303
PspGI CCWGG 2 cut(s) 276, 303
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 1 cut(s) 920
PsrI GAACNNNNNNTAC 2 cut(s) 366, 398
PsuI RGATCY 1 cut(s) 817
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 6 cut(s) 116, 182, 242, 510, 648, 700
RsaNI GTAC 6 cut(s) 115, 181, 241, 509, 647, 699
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 5 cut(s) 231, 332, 557, 744, 758
SatI GCNGC 2 cut(s) 416, 739
Sau3AI GATC 4 cut(s) 320, 774, 804, 817
Sau96I GGNCC 1 cut(s) 920
ScaI AGTACT 2 cut(s) 510, 700
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 2 cut(s) 278, 305
SfaNI GCATC 2 cut(s) 82, 317
SfuI TTCGAA 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SpeI ACTAGT 1 cut(s) 695
Sse9I AATT 6 cut(s) 8, 89, 457, 727, 755, 915
SspI AATATT 1 cut(s) 595
SspMI CTAG 2 cut(s) 440, 696
StyD4I CCNGG 2 cut(s) 276, 303
TaiI ACGT 1 cut(s) 839
TaqI TCGA 4 cut(s) 58, 87, 105, 189
TasI AATT 6 cut(s) 8, 89, 457, 727, 755, 915
TatI WGTACW 2 cut(s) 508, 698
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 5 cut(s) 231, 332, 557, 744, 758
Tru9I TTAA 5 cut(s) 231, 332, 557, 744, 758
TscAI CASTG 1 cut(s) 894
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 415, 738
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 387, 414, 509, 519, 665
TspGWI ACGGA 3 cut(s) 111, 115, 207
TspRI CASTG 1 cut(s) 894
Tth111I GACNNNGTC 1 cut(s) 446
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 2 cut(s) 8, 727
XceI RCATGY 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 2 cut(s) 440, 696
ZrmI AGTACT 2 cut(s) 510, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.