Rh5AG509900

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007439
Physical Location & Seq
Forward (+)
86098356 .. 86110084
11729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG509900.1

Sequence Viewer

Length: 1095 bp
ATGGACAAATTCTTAGTCCCTCTCAAACCCTCACCTCAACTTTCCACCAAACCCATTCGAAGGCGAAGATGGCATCGGAGCCTCATCGAATTGAACGGAAAGCTCGAACCCAAGTACCGGCATTCCGTCTCTGGTTTACTCATGCAATCCTATTCCGAGCTTAGGGCGTTTCGGCATTTGTACCACATCGACGGAAACCCGTGTCAAACCCATGTGAATCGGGTGGTGATTAATGGCACGTACATACCCCCAAGTTTAAAGGAAGGAGTTTCTGCCCTGGATTTTGACAACAAGGGGATATACCTGGCATCTGTGACAAGATCGGGGTGCTTAACGGTGCATGATTTTGAAACTCTTTATTGCCAGAGTAATGAACCATTGCTACGCTTGGAAGAAGATGAAGCAAAACATGTGCTGCACCTTTCTTTATATCAACAACTAGACTTTGTCCGGTGGAATTGTGCCAACCAAGATGAGGTTGTTTGCACATCTATGAAAAGGAATGAAGTACTTATATTTGACATTAGTTATATATCTTCTACACCATCTGAAGTTTTAAGAACAAAACCCCGTCCTGCTATCTTTGGGTCTAATATTCAGAAAGGTCTTTCTGATATTGCCTTTACCTCTTGTGAAGACTCAAGGGTACTTGCTTCTGATACAGATGGTGTTATTCATATATGGGACAGAAGAACTAGTACTTTTCCTTGTCTTGAACTTACGGCAAATTCCCGTGGCAGCCTTAATAGTATCCAATTAAATGTGGAAAATCAGATCATTTTTGGAGCTGGAAAGCATGGGGTGATCTATGCTTGGGATCTTCGTGGTGGAAGAACGTCTTCTTTCTTCAAGGGTTCTAAAGAGATATATGTGGTTGGATCATCAACTGACAACGGCATTCATATTTTGGATTTTTATCCAGATTCAAGCTCTCCTTGCCATGTGGACTACAATGAGGATGCACATCGTCTTTCTGGGGTAAAGGATCAAGATAAGCAAAACAGGTTTATTTCATTGTCTGAAGGAGTTACTGCATGTGCAGTTCATCCCCTCAACAGTACAATCATAACTGGAACAAAGTCTCCTATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

364

Amino Acids

40.91

Weight (kDa)

7.22

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 825, 886, 993
AcsI RAATTY 2 cut(s) 8, 727
AcuI CTGAAG 2 cut(s) 570, 1041
AfaI GTAC 7 cut(s) 116, 182, 242, 510, 648, 700, 1060
AfiI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 5 cut(s) 94, 350, 716, 850, 927
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 2 cut(s) 276, 303
AloI GAACNNNNNNTCC 1 cut(s) 1066
AluBI AGCT 4 cut(s) 103, 160, 788, 930
AluI AGCT 4 cut(s) 103, 160, 788, 930
Alw26I GTCTC 2 cut(s) 133, 1086
AlwI GGATC 3 cut(s) 825, 886, 993
ApeKI GCWGC 2 cut(s) 415, 738
ApoI RAATTY 2 cut(s) 8, 727
ArsI GACNNNNNNTTYG 1 cut(s) 32
AseI ATTAAT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 838
AsuHPI GGTGA 3 cut(s) 24, 238, 814
AsuII TTCGAA 1 cut(s) 58
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BarI GAAGNNNNNNTAC 2 cut(s) 682, 714
BbsI GAAGAC 2 cut(s) 642, 831
BbvI GCAGC 2 cut(s) 402, 750
BccI CCATC 3 cut(s) 63, 553, 659
BceAI ACGGC 2 cut(s) 738, 910
BciT130I CCWGG 2 cut(s) 278, 305
BciVI GTATCC 1 cut(s) 761
BcoDI GTCTC 2 cut(s) 133, 1086
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 2 cut(s) 440, 696
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 2 cut(s) 416, 739
BlsI GCNGC 2 cut(s) 417, 740
BmcAI AGTACT 2 cut(s) 510, 700
Bme1390I CCNGG 2 cut(s) 278, 305
BmiI GGNNCC 1 cut(s) 80
BmrFI CCNGG 2 cut(s) 278, 305
BmsI GCATC 3 cut(s) 82, 317, 949
BpiI GAAGAC 2 cut(s) 642, 831
Bpu10I CCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 58
BpuEI CTTGAG 1 cut(s) 625
BsaAI YACGTR 1 cut(s) 240
BsaBI GATNNNNATC 2 cut(s) 915, 963
BsaJI CCNNGG 2 cut(s) 276, 733
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 1 cut(s) 1066
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 1075
Bse3DI GCAATG 1 cut(s) 377
Bse8I GATNNNNATC 2 cut(s) 915, 963
BseBI CCWGG 2 cut(s) 278, 305
BseDI CCNNGG 2 cut(s) 276, 733
BseGI GGATG 2 cut(s) 964, 1045
BseJI GATNNNNATC 2 cut(s) 915, 963
BseLI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
BseMI GCAATG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 1075
BseXI GCAGC 2 cut(s) 402, 750
BsgI GTGCAG 2 cut(s) 401, 1059
BsiSI CCGG 2 cut(s) 118, 451
BslFI GGGAC 2 cut(s) 2, 698
BslI CCNNNNNNNGG 4 cut(s) 60, 117, 162, 475
BsmAI GTCTC 2 cut(s) 133, 1086
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 2, 698
BsmI GAATGC 2 cut(s) 121, 897
Bsp119I TTCGAA 1 cut(s) 58
Bsp143I GATC 6 cut(s) 320, 774, 804, 817, 878, 985
BspLI GGNNCC 1 cut(s) 80
BspPI GGATC 3 cut(s) 825, 886, 993
BspT104I TTCGAA 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 1 cut(s) 1075
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 276, 733
BssMI GATC 6 cut(s) 320, 774, 804, 817, 878, 985
Bst2UI CCWGG 2 cut(s) 278, 305
Bst4CI ACNGT 2 cut(s) 337, 1058
BstBAI YACGTR 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 13, 161
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 2 cut(s) 964, 1045
BstKTI GATC 6 cut(s) 323, 777, 807, 820, 881, 988
BstMAI GTCTC 2 cut(s) 133, 1086
BstMBI GATC 6 cut(s) 320, 774, 804, 817, 878, 985
BstMWI GCNNNNNNNGC 2 cut(s) 70, 936
BstNI CCWGG 2 cut(s) 278, 305
BstNSI RCATGY 2 cut(s) 413, 1038
BstSCI CCNGG 2 cut(s) 276, 303
BstV1I GCAGC 2 cut(s) 402, 750
BstV2I GAAGAC 2 cut(s) 642, 831
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
BsuI GTATCC 1 cut(s) 761
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 2 cut(s) 964, 1045
Cfr10I RCCGGY 1 cut(s) 117
Csp6I GTAC 7 cut(s) 115, 181, 241, 509, 647, 699, 1059
CviAII CATG 7 cut(s) 142, 212, 341, 410, 797, 941, 1035
CviJI RGCY 6 cut(s) 81, 103, 160, 741, 788, 930
CviKI_1 RGCY 6 cut(s) 81, 103, 160, 741, 788, 930
CviQI GTAC 7 cut(s) 115, 181, 241, 509, 647, 699, 1059
DdeI CTNAG 2 cut(s) 13, 161
DpnI GATC 6 cut(s) 322, 776, 806, 819, 880, 987
DpnII GATC 6 cut(s) 320, 774, 804, 817, 878, 985
DraI TTTAAA 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 570, 1041
EcoRII CCWGG 2 cut(s) 276, 303
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 7 cut(s) 145, 215, 344, 413, 800, 944, 1038
FalI AAGNNNNNCTT 4 cut(s) 823, 855, 919, 951
FaqI GGGAC 2 cut(s) 2, 698
FatI CATG 7 cut(s) 141, 211, 340, 409, 796, 940, 1034
Fnu4HI GCNGC 2 cut(s) 416, 739
FokI GGATG 2 cut(s) 971, 1032
Fsp4HI GCNGC 2 cut(s) 416, 739
FspBI CTAG 2 cut(s) 440, 696
GluI GCNGC 2 cut(s) 416, 739
HapII CCGG 2 cut(s) 118, 451
Hin1II CATG 7 cut(s) 145, 215, 344, 413, 800, 944, 1038
HinfI GANTC 3 cut(s) 217, 638, 923
HpaII CCGG 2 cut(s) 118, 451
HphI GGTGA 3 cut(s) 24, 238, 814
Hpy166II GTNNAC 2 cut(s) 137, 946
Hpy188I TCNGA 8 cut(s) 78, 157, 550, 600, 613, 658, 774, 1021
Hpy188III TCNNGA 3 cut(s) 713, 920, 989
Hpy8I GTNNAC 2 cut(s) 137, 946
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 54, 257, 1016
HpyCH4III ACNGT 2 cut(s) 337, 1058
HpyCH4IV ACGT 2 cut(s) 239, 836
HpyCH4V TGCA 7 cut(s) 145, 340, 418, 486, 962, 1034, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 936
HpyF3I CTNAG 2 cut(s) 13, 161
HpySE526I ACGT 2 cut(s) 239, 836
Hsp92II CATG 7 cut(s) 145, 215, 344, 413, 800, 944, 1038
Kzo9I GATC 6 cut(s) 320, 774, 804, 817, 878, 985
LmnI GCTCC 2 cut(s) 78, 785
Lsp1109I GCAGC 2 cut(s) 402, 750
LweI GCATC 3 cut(s) 82, 317, 949
MaeI CTAG 2 cut(s) 440, 696
MaeII ACGT 2 cut(s) 239, 836
MaeIII GTNAC 2 cut(s) 313, 1027
MalI GATC 6 cut(s) 322, 776, 806, 819, 880, 987
MboI GATC 6 cut(s) 320, 774, 804, 817, 878, 985
MflI RGATCY 1 cut(s) 817
MluCI AATT 5 cut(s) 8, 89, 457, 727, 755
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 1 cut(s) 856
MnlI CCTC 8 cut(s) 30, 40, 45, 92, 469, 637, 949, 1061
MroXI GAANNNNTTC 1 cut(s) 838
MseI TTAA 7 cut(s) 231, 257, 332, 557, 744, 758, 1093
MslI CAYNNNNRTG 1 cut(s) 491
MspI CCGG 2 cut(s) 118, 451
MspR9I CCNGG 2 cut(s) 278, 305
Mva1269I GAATGC 2 cut(s) 121, 897
MvaI CCWGG 2 cut(s) 278, 305
MwoI GCNNNNNNNGC 2 cut(s) 70, 936
NdeII GATC 6 cut(s) 320, 774, 804, 817, 878, 985
NlaIII CATG 7 cut(s) 145, 215, 344, 413, 800, 944, 1038
NlaIV GGNNCC 1 cut(s) 80
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 2 cut(s) 413, 1038
NspV TTCGAA 1 cut(s) 58
PciI ACATGT 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 2 cut(s) 121, 897
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 2 cut(s) 217, 923
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 417, 740
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 240
PscI ACATGT 1 cut(s) 409
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 276, 303
PspGI CCWGG 2 cut(s) 276, 303
PspN4I GGNNCC 1 cut(s) 80
PsrI GAACNNNNNNTAC 2 cut(s) 366, 398
PsuI RGATCY 1 cut(s) 817
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 7 cut(s) 116, 182, 242, 510, 648, 700, 1060
RsaNI GTAC 7 cut(s) 115, 181, 241, 509, 647, 699, 1059
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 7 cut(s) 231, 257, 332, 557, 744, 758, 1093
SatI GCNGC 2 cut(s) 416, 739
Sau3AI GATC 6 cut(s) 320, 774, 804, 817, 878, 985
ScaI AGTACT 2 cut(s) 510, 700
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 2 cut(s) 278, 305
SfaNI GCATC 3 cut(s) 82, 317, 949
SfuI TTCGAA 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SpeI ACTAGT 1 cut(s) 695
Sse9I AATT 5 cut(s) 8, 89, 457, 727, 755
SspI AATATT 1 cut(s) 595
SspMI CTAG 2 cut(s) 440, 696
StyD4I CCNGG 2 cut(s) 276, 303
TaaI ACNGT 2 cut(s) 337, 1058
TaiI ACGT 2 cut(s) 242, 839
TaqI TCGA 4 cut(s) 58, 87, 105, 189
TasI AATT 5 cut(s) 8, 89, 457, 727, 755
TatI WGTACW 3 cut(s) 508, 698, 1058
TfiI GAWTC 2 cut(s) 217, 923
Tru1I TTAA 7 cut(s) 231, 257, 332, 557, 744, 758, 1093
Tru9I TTAA 7 cut(s) 231, 257, 332, 557, 744, 758, 1093
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 415, 738
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 8 cut(s) 387, 414, 509, 519, 665, 890, 1002, 1034
TspGWI ACGGA 3 cut(s) 111, 115, 207
Tth111I GACNNNGTC 1 cut(s) 446
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 2 cut(s) 8, 727
XceI RCATGY 2 cut(s) 413, 1038
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 2 cut(s) 440, 696
ZrmI AGTACT 2 cut(s) 510, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.