MD11G1182700.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006137
Physical Location & Seq
Forward (+)
22532143 .. 22541112
8970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182700.v1.1.491

Sequence Viewer

Length: 3087 bp
ATGCAGACAATCTGTTTGAGTTTGATGCTATTGTTGTTGGTACTAGCAGTAACTGCATCGGGAGGAGGAGGGCAGTCAGATTTGGGAGCACTCTTAGACCTCCGGAAAGGCATTCACAAGGACCCTTCAGGGAAACTTCTTGTTTCGTGGGATTCTAACTCCTTGGACTCTGATGGCTGCCCCTTGAATTGGTTTGGGGTAACATGTAGTAATGGTCGCGTCATCTCAATTGCTATCAATGATGTTGGTTTGGTCGGTGACTTCAGTTTTTCAGCCATTACTGGCCTTACAATGCTTCAAAACTTGTCGCTTTCAAACAACCGGCTCACAGGGACCATCTCAAAGGTTGCTTTATTTCAGTCTCTTGAATACTTGGATCTCTCAAGCAATTTGTTTCATGGGCTATTACCCTACGATTTGGTCAACTTAAAGGGTCTAGTGCTTTTGAATCTTTCTTTGAACCAATTTGAAGGCATTTTGCCCTCTAGTTTTGGCAAACTTGAACAGTTGAAGTTTATTGATTTTCGAGCTAATGGCTTTTCTGGAGACATTATGAATTTTCTATCCAAAATGGGTAGCGTGGTCCATCTTGATGTAAGCAGCAATCTGTTATCTGGTTCATTAGATTTGGGACTTGGTAACTCCTCTTTTGTTTCTTCTATTCAGTACTTGAACGTTAGCCACAATTCCTTGGTCGGACAGCTTTTTCCTCACGATGGAATGCCATATTTTGATAGTTTGGAGGTGTTTGATGCTAGTTATAATCAGTTAGTGGGTCCTATACCTTCCTTCAATTTTGTTGTCTCTCTACGAGTACTTCGGCTTGGAAACAACCAGCTATCAGGTTCTCTACCGGAAGCTCTATTGCAAGAGAGCTCAATGCTCTTGTCAGAACTGGATCTTAGTCATAACGAACTTGAAGGTCCAGTAGGAAGCATCACCTCTGCAACTCTGAAGAAGTTTAATATTTCTTCAAACAAACTGTCAGGCTCCTTACCTGCCATGGTTGGGCATTGTTCTGTCATAGATCTGAGTAATAATATGCTCACAGGTAACTTGTCTCGAATCCGAGGTTGGGGAAATTACATTGAAGTTATCGAGCTAAGTTCCAATTCATTGACCGGAAGTCTGCCTAATGAAACGTCTCAATTTTTTAGGCTAACTTCATTTAAGATATCCAAGAACTCATTAGAAGGCATACTTCCGACAGTATTGGGAACATACCCAGAATTAAATGTGATTGATCTTAGCCTCAACCACCTACAAGGCTTACTTCTTCCAAGCTTTTTCTCTTCAACGAAATTGACTGATCTTAACTTATCGGGCAACAATCTGTCTGGGTCAATACCCATCCAGGACATATCAAGTGATTCTTCAAGTGGTTCGGCTCAAAATTTGAGCCTGGTGTCTATAGATCTGTCAAACAACTCATTGGCTGGTCATTTACCCCCAGAGATCATTAAGTTCCGTAGCTTAATGTATCTCGATCTATCTAATAACAACTTTGAAGGTAGCATTCCTGAGGACCTTCCAGATGTCCTGAAAGAGTTCAATGTTTCTTTCAATCATCTTTCTGGCGTCATACCTGAAAACTTGCGGCAGTTCCCTGATTCTGCATTTTATCCAGGGAACTCGTTGCTGATTTTTCCTCGTTCCCCATCTGCACCGAAGGATGTCCCAAACATGACTTTTAGGGAACACAGGCCCCTTATGAAAGCAGCTATTAGGATCTCCCTCATTGCAGGTTTGGTTGGTGGAGTTGCTGTTTTAGTTCTTTTATGCCTTATGATCCATTACAGGTCCCACTGGCAGAGATGTAGAAAGGGCAGTTCAAAAGCAAGTTCTGGAAAGAAAGATGCTGTGCAAGGAGGTTCTGCCCTTTCTCATCGTCATCGATCAGCACCGGACAAAAATGTAGACTGCTCAAAATCTTCTTGTGATCTCCTTCCAAAGCTTTCACCATCAACCCAAATGGGGTCTGCTCATGATGCCTGTGACACTTCGTCAGTTGTAAAGAATCCTAAGAATTTGGGTCATCCTGAATCAAAAGAAAGGGGTGAAGGGACATCTTCTCCTATGTCTCTCTTATCATCATCGAATCCATCACCCTCTAAAAAGCAACAGCCACCAGAGAGTCCTGCTGTGCTCACAGCTTATTCTCCGGATAAATTGGCTGGTGATTTGCATCTGTTTGATGGCTCCTTGGCCTTCACAGCAGAAGAACTTTCCTGTGCTCCAGCAGAAGCTATTGGAAGGAGTTGTCATGGGACAATGTACAAAGCAATGCTCGACTCCGGTCATGTATTGGCAGTCAAATGGCTACGGGAGGGAATAGCAAAAGGAAGGAAAGAATTTGCAAGAGAAGTGAAGAAACTAGGAAACATCAGGCACCCAAATCTAGTTTCTCTGCTGGGTTATTACTGGGGCCCTATAGAACATGAGAAACTGATTATATCAACTTATATCAATGCACAATCATTGGCATTCCATCTCCATGAGGCAGAGCGAACAAAACTCTCTCCCCTGTCACTTGAGGAACGGCTTAGGATTTCTGTAGATGTTGCCCGATGTCTGAACTTCCTGCATAATGAGAAGGCAATTCCCCATGGCAACCTCAAATCCACGAATATTTTATTAGAAACTCCTAGTCTGAATGCACTCCTTACAGATTATAGTCTCCACCGAATTTTGACTCCCGCTGGGACTACTGAGCAAGTCTTGAATGCAGGTGCTCTTGGCTATCGCCCCCCTGAATTTGCTAGCTCAAGTAAACCCTGCCCATCGTTGAAAAGTGATGTCTATGCATTTGGGGTGATCTTGTTGGAGCTCCTGACTGGAAAGAGTTCCGGGGAGATTGTCTCTGGGATACCGGGTGTGGTTGATCTGACAGACTGGGTGAGACTATTGGCAGAAGAAAACCGTTGTTTTGAATGCCTTGACAGACTGATTCTGGAAAGGCCGAGCGTAAAGAACTCCCCAAGAGTTCTTGATGGTATGCTACAGGTAGCTCTAAAATGTATTCTTCCAGCATCCGAAAGACCGGACATTAAAACCGTGTTCGAAGACATTTCAAGAATAGTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1029

Amino Acids

111.27

Weight (kDa)

6.27

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 70 1.3e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 386 - 445 2.3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 748 - 949 1.2e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 748 - 948 4.6e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
AarI CACCTGC 1 cut(s) 2717
Acc36I ACCTGC 3 cut(s) 1006, 1733, 2717
AccB1I GGYRCC 1 cut(s) 2388
AccI GTMKAC 1 cut(s) 1917
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 2 cut(s) 102, 2161
AciI CCGC 2 cut(s) 1597, 2697
AclI AACGTT 1 cut(s) 675
AclWI GGATC 4 cut(s) 384, 906, 1736, 1783
AcsI RAATTY 6 cut(s) 556, 1393, 2026, 2351, 2685, 2753
AcuI CTGAAG 3 cut(s) 111, 247, 974
AcyI GRCGYC 1 cut(s) 1578
AfaI GTAC 4 cut(s) 42, 668, 816, 2276
AfiI CCNNNNNNNGG 5 cut(s) 189, 716, 1008, 1075, 1974
AflIII ACRYGT 1 cut(s) 203
AhdI GACNNNNNGTC 2 cut(s) 1125, 2002
AjnI CCWGG 3 cut(s) 1353, 1401, 1624
AjuI GAANNNNNNNTTGG 4 cut(s) 1057, 1089, 2386, 2418
Alw21I GWGCWC 6 cut(s) 91, 878, 2148, 2236, 2734, 2829
Alw26I GTCTC 9 cut(s) 366, 540, 808, 1065, 1149, 2085, 2681, 2863, 2893
AlwI GGATC 4 cut(s) 384, 906, 1736, 1783
AlwNI CAGNNNCTG 1 cut(s) 53
Aor13HI TCCGGA 2 cut(s) 102, 2161
AoxI GGCC 5 cut(s) 283, 1703, 2205, 2425, 2957
ApaI GGGCCC 1 cut(s) 2429
ApeKI GCWGC 3 cut(s) 177, 600, 1718
ApoI RAATTY 6 cut(s) 556, 1393, 2026, 2351, 2685, 2753
ArsI GACNNNNNNTTYG 3 cut(s) 30, 968, 1000
Asp700I GAANNNNTTC 2 cut(s) 1547, 2842
AsuC2I CCSGG 2 cut(s) 2848, 2871
AsuHPI GGTGA 8 cut(s) 269, 931, 1950, 2069, 2097, 2189, 2824, 2908
AsuII TTCGAA 1 cut(s) 3060
AsuNHI GCTAGC 1 cut(s) 2759
AvaII GGWCC 7 cut(s) 121, 333, 583, 776, 923, 1525, 1800
AxyI CCTNAGG 1 cut(s) 1521
BaeGI GKGCMC 1 cut(s) 2429
BanI GGYRCC 1 cut(s) 2388
BanII GRGCYC 3 cut(s) 878, 2429, 2829
BbsI GAAGAC 1 cut(s) 3069
Bbv12I GWGCWC 6 cut(s) 91, 878, 2148, 2236, 2734, 2829
BbvI GCAGC 3 cut(s) 164, 612, 1730
BceAI ACGGC 1 cut(s) 2555
BciT130I CCWGG 3 cut(s) 1355, 1403, 1626
BciVI GTATCC 1 cut(s) 2859
BcnI CCSGG 2 cut(s) 2848, 2871
BcoDI GTCTC 9 cut(s) 366, 540, 808, 1065, 1149, 2085, 2681, 2863, 2893
BfaI CTAG 8 cut(s) 44, 437, 486, 756, 2375, 2399, 2646, 2760
BfmI CTRYAG 4 cut(s) 1410, 2430, 2553, 2999
BfuAI ACCTGC 3 cut(s) 1006, 1733, 2717
BfuI GTATCC 1 cut(s) 2859
BglII AGATCT 2 cut(s) 1027, 1414
BisI GCNGC 4 cut(s) 178, 601, 1598, 1719
BlsI GCNGC 4 cut(s) 179, 602, 1599, 1720
BmcAI AGTACT 2 cut(s) 668, 816
Bme1390I CCNGG 5 cut(s) 1355, 1403, 1626, 2848, 2871
Bme18I GGWCC 7 cut(s) 121, 333, 583, 776, 923, 1525, 1800
BmeRI GACNNNNNGTC 2 cut(s) 1125, 2002
BmrFI CCNGG 5 cut(s) 1355, 1403, 1626, 2848, 2871
BmrI ACTGGG 2 cut(s) 2431, 2902
BmsI GCATC 8 cut(s) 15, 65, 742, 945, 1846, 1978, 2194, 3038
BmtI GCTAGC 1 cut(s) 2763
BmuI ACTGGG 2 cut(s) 2431, 2902
BoxI GACNNNNGTC 1 cut(s) 2295
BpiI GAAGAC 1 cut(s) 3069
BplI GAGNNNNNCTC 2 cut(s) 2843, 2875
BpmI CTGGAG 2 cut(s) 564, 2220
Bpu10I CCTNAGC 1 cut(s) 2543
Bpu14I TTCGAA 1 cut(s) 3060
BpuEI CTTGAG 3 cut(s) 367, 2552, 2749
BpuMI CCSGG 2 cut(s) 2848, 2871
Bsa29I ATCGAT 1 cut(s) 1894
BsaBI GATNNNNATC 1 cut(s) 2184
BsaHI GRCGYC 1 cut(s) 1578
BsaJI CCNNGG 8 cut(s) 162, 690, 1002, 1069, 1625, 2202, 2605, 2847
BsaWI WCCGGW 7 cut(s) 102, 853, 1121, 1903, 2161, 2294, 3040
BsaXI ACNNNNNCTCC 4 cut(s) 1749, 1779, 2056, 2086
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 716, 1008, 1075, 1974
Bse118I RCCGGY 1 cut(s) 321
Bse1I ACTGG 7 cut(s) 286, 900, 926, 1811, 2426, 2839, 2897
Bse21I CCTNAGG 1 cut(s) 1521
Bse3DI GCAATG 2 cut(s) 1737, 2289
Bse8I GATNNNNATC 1 cut(s) 2184
BseAI TCCGGA 2 cut(s) 102, 2161
BseBI CCWGG 3 cut(s) 1355, 1403, 1626
BseCI ATCGAT 1 cut(s) 1894
BseDI CCNNGG 8 cut(s) 162, 690, 1002, 1069, 1625, 2202, 2605, 2847
BseGI GGATG 4 cut(s) 1350, 1678, 2035, 3029
BseJI GATNNNNATC 1 cut(s) 2184
BseLI CCNNNNNNNGG 5 cut(s) 189, 716, 1008, 1075, 1974
BseMI GCAATG 2 cut(s) 1737, 2289
BseMII CTCAG 3 cut(s) 1022, 1512, 2700
BseNI ACTGG 7 cut(s) 286, 900, 926, 1811, 2426, 2839, 2897
BseRI GAGGAG 3 cut(s) 78, 81, 634
BseSI GKGCMC 1 cut(s) 2429
BseXI GCAGC 3 cut(s) 164, 612, 1730
BseYI CCCAGC 2 cut(s) 2410, 2699
BsgI GTGCAG 1 cut(s) 1647
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 285, 1705, 2207, 2427, 2959
BshNI GGYRCC 1 cut(s) 2388
BshVI ATCGAT 1 cut(s) 1894
BsiHKAI GWGCWC 6 cut(s) 91, 878, 2148, 2236, 2734, 2829
BslFI GGGAC 7 cut(s) 346, 645, 1661, 1786, 2077, 2281, 2716
BslI CCNNNNNNNGG 5 cut(s) 189, 716, 1008, 1075, 1974
BsmAI GTCTC 9 cut(s) 366, 540, 808, 1065, 1149, 2085, 2681, 2863, 2893
BsmBI CGTCTC 1 cut(s) 1149
BsmFI GGGAC 7 cut(s) 346, 645, 1661, 1786, 2077, 2281, 2716
BsmI GAATGC 7 cut(s) 111, 726, 1515, 2483, 2659, 2728, 2936
BsnI GGCC 5 cut(s) 285, 1705, 2207, 2427, 2959
Bsp119I TTCGAA 1 cut(s) 3060
Bsp120I GGGCCC 1 cut(s) 2425
Bsp1286I GDGCHC 7 cut(s) 91, 878, 2148, 2236, 2429, 2734, 2829
Bsp13I TCCGGA 2 cut(s) 102, 2161
Bsp1407I TGTACA 1 cut(s) 2274
Bsp19I CCATGG 2 cut(s) 1002, 2605
BspACI CCGC 2 cut(s) 1597, 2697
BspANI GGCC 5 cut(s) 285, 1705, 2207, 2427, 2959
BspCNI CTCAG 3 cut(s) 1023, 1513, 2701
BspDI ATCGAT 1 cut(s) 1894
BspEI TCCGGA 2 cut(s) 102, 2161
BspFNI CGCG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 1984
BspMI ACCTGC 3 cut(s) 1006, 1733, 2717
BspOI GCTAGC 1 cut(s) 2763
BspPI GGATC 4 cut(s) 384, 906, 1736, 1783
BspT104I TTCGAA 1 cut(s) 3060
BspT107I GGYRCC 1 cut(s) 2388
BsrDI GCAATG 2 cut(s) 1737, 2289
BsrFI RCCGGY 1 cut(s) 321
BsrGI TGTACA 1 cut(s) 2274
BsrI ACTGG 7 cut(s) 286, 900, 926, 1811, 2426, 2839, 2897
BssAI RCCGGY 1 cut(s) 321
BssECI CCNNGG 8 cut(s) 162, 690, 1002, 1069, 1625, 2202, 2605, 2847
BssNI GRCGYC 1 cut(s) 1578
BssT1I CCWWGG 5 cut(s) 162, 690, 1002, 2202, 2605
Bst2UI CCWGG 3 cut(s) 1355, 1403, 1626
Bst4CI ACNGT 5 cut(s) 507, 984, 1210, 2921, 3055
Bst6I CTCTTC 1 cut(s) 1297
BstACI GRCGYC 1 cut(s) 1578
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 2274
BstBI TTCGAA 1 cut(s) 3060
BstC8I GCNNGC 1 cut(s) 2761
BstDEI CTNAG 9 cut(s) 94, 902, 1031, 1103, 1247, 1521, 2022, 2543, 2709
BstDSI CCRYGG 2 cut(s) 1002, 2605
BstF5I GGATG 4 cut(s) 1350, 1678, 2035, 3029
BstFNI CGCG 1 cut(s) 219
BstMAI GTCTC 9 cut(s) 366, 540, 808, 1065, 1149, 2085, 2681, 2863, 2893
BstMWI GCNNNNNNNGC 3 cut(s) 53, 1988, 2213
BstNI CCWGG 3 cut(s) 1355, 1403, 1626
BstNSI RCATGY 1 cut(s) 207
BstPAI GACNNNNGTC 1 cut(s) 2295
BstSCI CCNGG 5 cut(s) 1353, 1401, 1624, 2846, 2869
BstSFI CTRYAG 4 cut(s) 1410, 2430, 2553, 2999
BstSLI GKGCMC 1 cut(s) 2429
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 164, 612, 1730
BstV2I GAAGAC 1 cut(s) 3069
BstX2I RGATCY 5 cut(s) 376, 898, 1027, 1414, 1728
BstYI RGATCY 5 cut(s) 376, 898, 1027, 1414, 1728
Bsu15I ATCGAT 1 cut(s) 1894
Bsu36I CCTNAGG 1 cut(s) 1521
BsuI GTATCC 1 cut(s) 2859
BsuRI GGCC 5 cut(s) 285, 1705, 2207, 2427, 2959
BsuTUI ATCGAT 1 cut(s) 1894
BtgI CCRYGG 2 cut(s) 1002, 2605
BtsCI GGATG 4 cut(s) 1350, 1678, 2035, 3029
BtsIMutI CAGTG 1 cut(s) 1804
BveI ACCTGC 3 cut(s) 1006, 1733, 2717
Cac8I GCNNGC 1 cut(s) 2761
CaiI CAGNNNCTG 1 cut(s) 53
CciI TCATGA 1 cut(s) 1984
Cfr10I RCCGGY 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 1894
CseI GACGC 2 cut(s) 208, 1567
Csp6I GTAC 4 cut(s) 41, 667, 815, 2275
CviQI GTAC 4 cut(s) 41, 667, 815, 2275
DdeI CTNAG 9 cut(s) 94, 902, 1031, 1103, 1247, 1521, 2022, 2543, 2709
DriI GACNNNNNGTC 2 cut(s) 1125, 2002
Eam1104I CTCTTC 1 cut(s) 1297
Eam1105I GACNNNNNGTC 2 cut(s) 1125, 2002
EarI CTCTTC 1 cut(s) 1297
Ecl136II GAGCTC 2 cut(s) 876, 2827
Eco130I CCWWGG 5 cut(s) 162, 690, 1002, 2202, 2605
Eco24I GRGCYC 3 cut(s) 878, 2429, 2829
Eco32I GATATC 1 cut(s) 1176
Eco47I GGWCC 7 cut(s) 121, 333, 583, 776, 923, 1525, 1800
Eco53kI GAGCTC 2 cut(s) 876, 2827
Eco57I CTGAAG 3 cut(s) 111, 247, 974
Eco81I CCTNAGG 1 cut(s) 1521
EcoICRI GAGCTC 2 cut(s) 876, 2827
EcoO109I RGGNCCY 7 cut(s) 121, 776, 1525, 1704, 1800, 2425, 2426
EcoRII CCWGG 3 cut(s) 1353, 1401, 1624
EcoRV GATATC 1 cut(s) 1176
EcoT14I CCWWGG 5 cut(s) 162, 690, 1002, 2202, 2605
EcoT22I ATGCAT 1 cut(s) 2806
EcoT38I GRGCYC 3 cut(s) 878, 2429, 2829
ErhI CCWWGG 5 cut(s) 162, 690, 1002, 2202, 2605
Esp3I CGTCTC 1 cut(s) 1149
FalI AAGNNNNNCTT 6 cut(s) 1185, 1217, 1257, 1289, 1357, 1389
FaqI GGGAC 7 cut(s) 346, 645, 1661, 1786, 2077, 2281, 2716
FauI CCCGC 1 cut(s) 2704
FblI GTMKAC 1 cut(s) 1917
Fnu4HI GCNGC 4 cut(s) 178, 601, 1598, 1719
FokI GGATG 4 cut(s) 1337, 1685, 2022, 3016
FriOI GRGCYC 3 cut(s) 878, 2429, 2829
Fsp4HI GCNGC 4 cut(s) 178, 601, 1598, 1719
FspBI CTAG 8 cut(s) 44, 437, 486, 756, 2375, 2399, 2646, 2760
GluI GCNGC 4 cut(s) 178, 601, 1598, 1719
GsaI CCCAGC 2 cut(s) 2414, 2703
GsuI CTGGAG 2 cut(s) 564, 2220
HaeIII GGCC 5 cut(s) 285, 1705, 2207, 2427, 2959
HgaI GACGC 2 cut(s) 208, 1567
Hin1I GRCGYC 1 cut(s) 1578
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 2 cut(s) 1282, 1952
HphI GGTGA 8 cut(s) 269, 931, 1950, 2069, 2097, 2189, 2824, 2908
Hpy166II GTNNAC 4 cut(s) 424, 1918, 2771, 3082
Hpy8I GTNNAC 4 cut(s) 424, 1918, 2771, 3082
HpyCH4III ACNGT 5 cut(s) 507, 984, 1210, 2921, 3055
HpyCH4IV ACGT 2 cut(s) 675, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 1988, 2213
HpyF3I CTNAG 9 cut(s) 94, 902, 1031, 1103, 1247, 1521, 2022, 2543, 2709
HpySE526I ACGT 2 cut(s) 675, 1142
Hsp92I GRCGYC 1 cut(s) 1578
Kpn2I TCCGGA 2 cut(s) 102, 2161
LmnI GCTCC 6 cut(s) 86, 995, 2204, 2239, 2824, 2832
Lsp1109I GCAGC 3 cut(s) 164, 612, 1730
LweI GCATC 8 cut(s) 15, 65, 742, 945, 1846, 1978, 2194, 3038
MaeI CTAG 8 cut(s) 44, 437, 486, 756, 2375, 2399, 2646, 2760
MaeII ACGT 2 cut(s) 675, 1142
MaeIII GTNAC 7 cut(s) 49, 199, 257, 638, 1052, 1994, 2526
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 5 cut(s) 376, 898, 1027, 1414, 1728
MhlI GDGCHC 7 cut(s) 91, 878, 2148, 2236, 2429, 2734, 2829
MlyI GAGTC 4 cut(s) 161, 2143, 2285, 2686
MmeI TCCRAC 3 cut(s) 676, 1229, 2802
Mph1103I ATGCAT 1 cut(s) 2806
MroI TCCGGA 2 cut(s) 102, 2161
MroXI GAANNNNTTC 2 cut(s) 1547, 2842
MseI TTAA 8 cut(s) 428, 963, 1170, 1232, 1314, 1461, 1475, 3048
MslI CAYNNNNRTG 2 cut(s) 591, 2493
MspA1I CMGCKG 1 cut(s) 2699
MspR9I CCNGG 5 cut(s) 1355, 1403, 1626, 2848, 2871
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 7 cut(s) 111, 726, 1515, 2483, 2659, 2728, 2936
MvaI CCWGG 3 cut(s) 1355, 1403, 1626
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 3 cut(s) 53, 1988, 2213
NciI CCSGG 2 cut(s) 2848, 2871
NcoI CCATGG 2 cut(s) 1002, 2605
NheI GCTAGC 1 cut(s) 2759
NmeAIII GCCGAG 1 cut(s) 2985
NmuCI GTSAC 3 cut(s) 257, 1994, 2526
NsiI ATGCAT 1 cut(s) 2806
NspI RCATGY 1 cut(s) 207
NspV TTCGAA 1 cut(s) 3060
PagI TCATGA 1 cut(s) 1984
PaqCI CACCTGC 1 cut(s) 2717
PciI ACATGT 1 cut(s) 203
PctI GAATGC 7 cut(s) 111, 726, 1515, 2483, 2659, 2728, 2936
PdmI GAANNNNTTC 2 cut(s) 1547, 2842
PfeI GAWTC 9 cut(s) 152, 448, 1065, 1370, 1610, 2017, 2042, 2098, 2947
PfoI TCCNGGA 1 cut(s) 1353
PkrI GCNGC 4 cut(s) 179, 602, 1599, 1720
PleI GAGTC 4 cut(s) 161, 2142, 2285, 2686
PpsI GAGTC 4 cut(s) 161, 2142, 2285, 2686
PpuMI RGGWCCY 4 cut(s) 121, 776, 1525, 1800
PscI ACATGT 1 cut(s) 203
PshAI GACNNNNGTC 1 cut(s) 2295
PsiI TTATAA 1 cut(s) 762
Psp124BI GAGCTC 2 cut(s) 878, 2829
Psp1406I AACGTT 1 cut(s) 675
Psp5II RGGWCCY 4 cut(s) 121, 776, 1525, 1800
Psp6I CCWGG 3 cut(s) 1353, 1401, 1624
PspFI CCCAGC 2 cut(s) 2410, 2699
PspGI CCWGG 3 cut(s) 1353, 1401, 1624
PspOMI GGGCCC 1 cut(s) 2425
PspPPI RGGWCCY 4 cut(s) 121, 776, 1525, 1800
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 5 cut(s) 376, 898, 1027, 1414, 1728
RsaI GTAC 4 cut(s) 42, 668, 816, 2276
RsaNI GTAC 4 cut(s) 41, 667, 815, 2275
RseI CAYNNNNRTG 2 cut(s) 591, 2493
SacI GAGCTC 2 cut(s) 878, 2829
SaqAI TTAA 8 cut(s) 428, 963, 1170, 1232, 1314, 1461, 1475, 3048
SatI GCNGC 4 cut(s) 178, 601, 1598, 1719
ScaI AGTACT 2 cut(s) 668, 816
SchI GAGTC 4 cut(s) 161, 2143, 2285, 2686
ScrFI CCNGG 5 cut(s) 1355, 1403, 1626, 2848, 2871
SduI GDGCHC 7 cut(s) 91, 878, 2148, 2236, 2429, 2734, 2829
SfaNI GCATC 8 cut(s) 15, 65, 742, 945, 1846, 1978, 2194, 3038
SfcI CTRYAG 4 cut(s) 1410, 2430, 2553, 2999
SfuI TTCGAA 1 cut(s) 3060
SinI GGWCC 7 cut(s) 121, 333, 583, 776, 923, 1525, 1800
SmiMI CAYNNNNRTG 2 cut(s) 591, 2493
SmlI CTYRAG 3 cut(s) 382, 2531, 2764
SmoI CTYRAG 3 cut(s) 382, 2531, 2764
SsiI CCGC 2 cut(s) 1597, 2697
SspI AATATT 2 cut(s) 967, 2629
SspMI CTAG 8 cut(s) 44, 437, 486, 756, 2375, 2399, 2646, 2760
SstI GAGCTC 2 cut(s) 878, 2829
StyD4I CCNGG 5 cut(s) 1353, 1401, 1624, 2846, 2869
StyI CCWWGG 5 cut(s) 162, 690, 1002, 2202, 2605
TaaI ACNGT 5 cut(s) 507, 984, 1210, 2921, 3055
TaiI ACGT 2 cut(s) 678, 1145
TaqI TCGA 8 cut(s) 526, 1063, 1098, 1485, 1894, 2096, 2289, 3060
TatI WGTACW 3 cut(s) 666, 814, 2274
TauI GCSGC 1 cut(s) 1600
TfiI GAWTC 9 cut(s) 152, 448, 1065, 1370, 1610, 2017, 2042, 2098, 2947
Tru1I TTAA 8 cut(s) 428, 963, 1170, 1232, 1314, 1461, 1475, 3048
Tru9I TTAA 8 cut(s) 428, 963, 1170, 1232, 1314, 1461, 1475, 3048
TscAI CASTG 1 cut(s) 1811
TseFI GTSAC 3 cut(s) 257, 1994, 2526
TseI GCWGC 3 cut(s) 177, 600, 1718
Tsp45I GTSAC 3 cut(s) 257, 1994, 2526
TspDTI ATGAA 7 cut(s) 386, 569, 609, 1104, 1152, 1155, 1727
TspGWI ACGGA 1 cut(s) 1457
TspRI CASTG 1 cut(s) 1811
VpaK11BI GGWCC 7 cut(s) 121, 333, 583, 776, 923, 1525, 1800
XapI RAATTY 6 cut(s) 556, 1393, 2026, 2351, 2685, 2753
XceI RCATGY 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 1917
XmnI GAANNNNTTC 2 cut(s) 1547, 2842
XspI CTAG 8 cut(s) 44, 437, 486, 756, 2375, 2399, 2646, 2760
ZrmI AGTACT 2 cut(s) 668, 816
Zsp2I ATGCAT 1 cut(s) 2806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.