Rw5G031080
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006137
Physical Location & Seq
Forward (+)
50404132 .. 50409460
5329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031080.1

Sequence Viewer

Length: 3012 bp
ATGCAGACAATATGCTTCATGTTTTTGTTGTTGTTGTTGGTAGTAATTGCAGCATCAGGGCAGTCAGATTTAGATGCACTCTTAGAGCTCAAAAAAGGGATTCAGAAAGACCCTTCTGGCCAAGTTCTTGTTTCATGGGATTCAAATTCCTTGGCCTCTGATGGATGCCCCCTCAATTGGTTTGGGATAGTTTGTAGTGATGGCCTTGTCACATCCATTTCTATAAACGATATTGGCCTGGTTGGCGAGTTCAGATTTTCAGCCATTGCTGGCCTTAAAGTGCTGCGCAATTTATCACTCTCAAACAACCAGCTCACAGGGGCTCTCTCAAAGGTCTCTCTGATTCAGTCTCTAGAGCATTTAGATCTCTCACGCAATTTGTTTAATGGGTTAATACCATCTGGCTTAGCAGACTTAAAGCATTTAGCGCTTTTGAATCTGTCTTCAAACCAATTTGAAGGCATTTTCCCCTCTGGTTTTGGGAAACTTGAACAGCTGAGGTACATTGATGTTCGAGATAATGCCTTGTCTGGGGATATCATGGCTTTTCTATCCCAAATGGGTAGCGTGGTTCATGTTGATCTAAGTAGCAATCTGTTTTCTGGTTCACTGGATTTGGAAATTGGTAACTCCTCTTTTGTTTCTTCTGTTCAATATTTGAATGTAAGTCACAATTCCTTGGCTGGAGAGCTTTTTCCTCACGATGGAATGCCATATTTTGATAGTTTGGAGGTGTTTGATGCTAGTGATAATCAGTTAGTTGGTCCTATACCTTCCTTCAATTTTGTTGTCTCTCTTCGGATACTTCGGCTCGGAAGCAACCAGCTATCAGGTTCTCTACCGGAAGCTCTGTTGCAAGGAAGCTCAATGCTCTTGTCTGAATTGGATCTTAGTCTTAATCATCTTGAAGGTCCAGTAGGAAGCATAACCTCTGCAACTCTGAAGAAGCTGAATATATCTTCAAATAAATTATCAGGGTCCTTGCCTGCCAATGTTGGCCACTGTGCCATCGTAGACCTCAGTAATAATATGCTCACTGGTAACCTGTCCCGAATCCGTAGTTGGGGAAATTACATTGAAGTTATCCAACTAAGTTCAAATTCTTTGACAGGAAGCTTGCCTGGCCAAACATCCCAATTTTTTAGGCTAACTTCTTTTAAGATATCCAATAACTCATTAGAGGGTGTTCTTCCATCAGTATTGGGTACGTACCCAGAACTAAAAGTGATAGATCTTAGCCTCAACCGGTTGCAAGGTTTTCTCCTTCCAAGCTTTTTCTCTTCAACAATATTGACTGATCTAAACTTGTCAGGCAACAATTTCTCTGGGTCATACCTCCTGGTGTCTCTAGATCTGTCAAATAATTCATTGAGTGGTCATTTGCCCCAAGAATTCAGCAAGTTTCATAGCTTGGTATATCTCAACCTGTCTGGTAATGATTTTGAAGGTAGGATTCCTGAGAACCTTCCAGATGAACTGAAAGAATTCAACGTCTCTTTGAATAATCTTTCTGGTCTTGTACCTGAAAATTTAAGGCACTTCCCTGTTTCAGCATTTTATCCAGGGAATTCTTTGCTGATGTTTCCTCATCCCCTATCATCGCCAAACAATGTCCCTGATATGATCTCTAGAAATCACAGGTCTCCTATAAAAGCTGCTATTAGAATTGCCCTGATTGTAGGTTTGCTTGGTGGTGGTGCTATTGTAGCCCTCTCATGCATGATGATCTATTATAGGGCCCACTGTCAAGGATGTAGAAAGAGAGGTCAAAAAGCAAGCTGTGAAAAGAATGGCGTTGTGCAAGGGGACTCTTCCCTTTCTCATAGATCAGTACCTGGCAAAACTGAAGACTTAAAATCTTCATATGGTTTCCTTCAAGACCCTTCGCCATCATCCGCAAGGGAGTCTACTCGTAATGCCGGTGACACTTCATCAGTTCTAGAGAAGTCTAAACAATTAGGTCATCCTGAATCAACAAAGCTAGAGGAAGGGGTATCTTCTCCCATGTCTCTCTTATCGCCTTCAAACCCATTGCCTTCGAAAAGCCAAGAACCACTCAATAGTTCTGCTGTTCTCAACACTTGTTCTCCAGACAAACTAGCTGGTGATTTACATCTGTTTGATGGCTCCCTGGCATTCACAGCAGAAGAACTTTCATGTGCTCCCGCAGAAGCTATTGGAAGGAGTTGTCATGGAACAATGTACAAAGCAATGCTCGAGTCTGGTCATGTAATTGCTGTCAAATGGTTAAGGGAGGGAATAGCAAAAGGAAGGAAAGAATTTGCTAGAGAAGTAAAGAAACTTGGGAGCATCCGCCATCCAAATCTAGTTTCTTTGCAGGGTTACTATTGGGGCCCAAAGGAGCATGAAAACTTATTATATCAAATTATATCAATGCTGAATCTTTGGCCTACTATCTCCATGGTTGAGCCAAGAAAACTCTCACCCTTGTCTCTCGAGGAACGGCTCAAGGTTTCTGTAGATGTTGCCCGATGTCTGAACTACCTCCATAATGAGAAGGCAATTCCCCATGGCAACCTCAAATCCACAAATATTCTACTAGAAACTCCTAATCATAATGCACTCCTTACAGATTATAGTATCCACCGAATATTGACTCCTGCTGGAACTACTGAGCAAGTTCTGAATGCAGGTGCTCTTGGCTATCGGCCCCCTGAATTTGCTAGTTCAAGTAGACCCTGCCCCTCATTGAAGAGTGACGTCTATGCATTTGGAGTGATCTTGTTGGAGCTGCTGACTGGAAAGAGTTCTGGGGAGATTGTCTCTGGCATACCAGGTGTGGTTGATCTGACTGATTGGGTGAGATTTTTGGCAGAAGAAAACCGTTCTTTTGAATGCCTTGACAGACTGATTCTGGAAAAGCACAGTGTTAAGCACTCGCCTAGAGTTCTTGATGAATTGCTCCAGGTAGCTTTAAGATGTACCCTTCCAGCATCCGAGAGACCCGACATTAAAACTGTGTTTGAAGACCTTTCAAAATTAGTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1003

Amino Acids

108.95

Weight (kDa)

6.03

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 22 - 66 4e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 95 - 152 5.1e-06 Leucine rich repeat
LRR_8 PF13855 117 - 176 2e-09 Leucine rich repeat
LRR_8 PF13855 141 - 199 2.1e-06 Leucine rich repeat
LRR_8 PF13855 382 - 441 1.1e-06 Leucine rich repeat
LRR_8 PF13855 441 - 481 9.8e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 724 - 997 1.2e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 724 - 923 2e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2642
AatII GACGTC 1 cut(s) 2724
Acc16I TGCGCA 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 2642
AccI GTMKAC 3 cut(s) 1014, 1907, 2695
AciI CCGC 3 cut(s) 1896, 2166, 2314
AclWI GGATC 1 cut(s) 894
AcoI YGGCCR 3 cut(s) 118, 997, 1123
AcsI RAATTY 8 cut(s) 145, 1099, 1391, 1484, 1528, 1567, 2279, 2678
AcuI CTGAAG 2 cut(s) 962, 1866
AcyI GRCGYC 1 cut(s) 2721
AfaI GTAC 8 cut(s) 503, 1207, 1211, 1521, 1833, 2204, 2944, 3008
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 4 cut(s) 177, 531, 704, 1063
AgeI ACCGGT 1 cut(s) 1245
AjnI CCWGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
AjuI GAANNNNNNNTTGG 4 cut(s) 1045, 1077, 2314, 2346
Alw21I GWGCWC 3 cut(s) 90, 2164, 2659
AlwI GGATC 1 cut(s) 894
AlwNI CAGNNNCTG 1 cut(s) 1835
Ama87I CYCGRG 2 cut(s) 2216, 2456
Aor51HI AGCGCT 1 cut(s) 429
ApaI GGGCCC 2 cut(s) 1741, 2357
ApeKI GCWGC 4 cut(s) 50, 283, 1655, 2752
ApoI RAATTY 8 cut(s) 145, 1099, 1391, 1484, 1528, 1567, 2279, 2678
AsiGI ACCGGT 1 cut(s) 1245
Asp700I GAANNNNTTC 2 cut(s) 1484, 2767
AspLEI GCGC 2 cut(s) 288, 430
AspS9I GGNCC 8 cut(s) 764, 911, 978, 1737, 1738, 2353, 2354, 2670
AsuHPI GGTGA 4 cut(s) 1934, 2117, 2436, 2833
AsuII TTCGAA 1 cut(s) 2039
AvaI CYCGRG 2 cut(s) 2216, 2456
AvaII GGWCC 3 cut(s) 764, 911, 978
BaeGI GKGCMC 2 cut(s) 1741, 2357
BalI TGGCCA 3 cut(s) 120, 999, 1125
BanII GRGCYC 4 cut(s) 90, 325, 1741, 2357
BbsI GAAGAC 3 cut(s) 435, 1854, 2994
Bbv12I GWGCWC 3 cut(s) 90, 2164, 2659
BbvCI CCTCAGC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 62, 270, 1642, 2739
BccI CCATC 9 cut(s) 155, 194, 406, 698, 1016, 1201, 1897, 2117, 2325
BceAI ACGGC 1 cut(s) 2480
BciT130I CCWGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
BciVI GTATCC 2 cut(s) 795, 2612
BfmI CTRYAG 1 cut(s) 2478
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 2642
BfuI GTATCC 2 cut(s) 795, 2612
BglI GCCNNNNNGGC 1 cut(s) 243
BglII AGATCT 3 cut(s) 364, 1231, 1351
BisI GCNGC 4 cut(s) 51, 284, 1656, 2753
BlpI GCTNAGC 1 cut(s) 406
BlsI GCNGC 4 cut(s) 52, 285, 1657, 2754
Bme1390I CCNGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
Bme18I GGWCC 3 cut(s) 764, 911, 978
BmeT110I CYCGRG 2 cut(s) 2216, 2456
BmgT120I GGNCC 8 cut(s) 764, 911, 978, 1737, 1738, 2353, 2354, 2670
BmiI GGNNCC 6 cut(s) 979, 1739, 2128, 2354, 2355, 2672
BmrFI CCNGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
BmsI GCATC 6 cut(s) 62, 64, 155, 730, 2319, 2963
BpiI GAAGAC 3 cut(s) 435, 1854, 2994
BplI GAGNNNNNCTC 2 cut(s) 2768, 2800
BpmI CTGGAG 3 cut(s) 705, 2073, 2909
Bpu10I CCTNAGC 1 cut(s) 497
Bpu1102I GCTNAGC 1 cut(s) 406
Bpu14I TTCGAA 1 cut(s) 2039
BpuEI CTTGAG 1 cut(s) 2453
BsaAI YACGTR 1 cut(s) 1209
BsaBI GATNNNNATC 1 cut(s) 2112
BsaHI GRCGYC 1 cut(s) 2721
BsaI GGTCTC 3 cut(s) 340, 1647, 2956
BsaJI CCNNGG 6 cut(s) 150, 678, 1562, 2130, 2421, 2530
BsaWI WCCGGW 2 cut(s) 841, 1245
BsaXI ACNNNNNCTCC 2 cut(s) 2071, 2101
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 531, 704, 1063
Bse118I RCCGGY 2 cut(s) 1245, 1919
Bse1I ACTGG 4 cut(s) 615, 914, 1042, 2764
Bse3DI GCAATG 3 cut(s) 264, 2030, 2217
Bse8I GATNNNNATC 1 cut(s) 2112
BseBI CCWGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
BseDI CCNNGG 6 cut(s) 150, 678, 1562, 2130, 2421, 2530
BseJI GATNNNNATC 1 cut(s) 2112
BseLI CCNNNNNNNGG 4 cut(s) 177, 531, 704, 1063
BseMI GCAATG 3 cut(s) 264, 2030, 2217
BseMII CTCAG 4 cut(s) 488, 1033, 1449, 2625
BseNI ACTGG 4 cut(s) 615, 914, 1042, 2764
BseRI GAGGAG 1 cut(s) 622
BseSI GKGCMC 2 cut(s) 1741, 2357
BseXI GCAGC 4 cut(s) 62, 270, 1642, 2739
BshTI ACCGGT 1 cut(s) 1245
BsiHKAI GWGCWC 3 cut(s) 90, 2164, 2659
BsiHKCI CYCGRG 2 cut(s) 2216, 2456
BsiSI CCGG 3 cut(s) 842, 1246, 1920
BslFI GGGAC 3 cut(s) 1033, 1598, 1820
BslI CCNNNNNNNGG 4 cut(s) 177, 531, 704, 1063
BsmBI CGTCTC 1 cut(s) 1498
BsmFI GGGAC 3 cut(s) 1033, 1598, 1820
BsmI GAATGC 4 cut(s) 714, 2135, 2653, 2861
Bso31I GGTCTC 3 cut(s) 340, 1647, 2956
BsoBI CYCGRG 2 cut(s) 2216, 2456
Bsp119I TTCGAA 1 cut(s) 2039
Bsp120I GGGCCC 2 cut(s) 1737, 2353
Bsp1286I GDGCHC 6 cut(s) 90, 325, 1741, 2164, 2357, 2659
Bsp1407I TGTACA 1 cut(s) 2202
Bsp1720I GCTNAGC 1 cut(s) 406
Bsp19I CCATGG 2 cut(s) 2421, 2530
BspACI CCGC 3 cut(s) 1896, 2166, 2314
BspCNI CTCAG 4 cut(s) 489, 1032, 1450, 2626
BspLI GGNNCC 6 cut(s) 979, 1739, 2128, 2354, 2355, 2672
BspMI ACCTGC 1 cut(s) 2642
BspPI GGATC 1 cut(s) 894
BspT104I TTCGAA 1 cut(s) 2039
BspTNI GGTCTC 3 cut(s) 340, 1647, 2956
BsrDI GCAATG 3 cut(s) 264, 2030, 2217
BsrFI RCCGGY 2 cut(s) 1245, 1919
BsrGI TGTACA 1 cut(s) 2202
BsrI ACTGG 4 cut(s) 615, 914, 1042, 2764
BssAI RCCGGY 2 cut(s) 1245, 1919
BssECI CCNNGG 6 cut(s) 150, 678, 1562, 2130, 2421, 2530
BssNI GRCGYC 1 cut(s) 2721
BssT1I CCWWGG 4 cut(s) 150, 678, 2421, 2530
Bst2UI CCWGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
Bst4CI ACNGT 5 cut(s) 1004, 1745, 2846, 2888, 2980
Bst6I CTCTTC 4 cut(s) 801, 1285, 1816, 2708
BstACI GRCGYC 1 cut(s) 2721
BstAUI TGTACA 1 cut(s) 2202
BstBAI YACGTR 1 cut(s) 1209
BstBI TTCGAA 1 cut(s) 2039
BstC8I GCNNGC 4 cut(s) 271, 987, 1118, 1777
BstDSI CCRYGG 2 cut(s) 2421, 2530
BstEII GGTNACC 1 cut(s) 1040
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 288, 430
BstMWI GCNNNNNNNGC 5 cut(s) 243, 427, 1122, 1706, 2141
BstNI CCWGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
BstSFI CTRYAG 1 cut(s) 2478
BstSLI GKGCMC 2 cut(s) 1741, 2357
BstSNI TACGTA 1 cut(s) 1209
BstV1I GCAGC 4 cut(s) 62, 270, 1642, 2739
BstV2I GAAGAC 3 cut(s) 435, 1854, 2994
BstX2I RGATCY 4 cut(s) 364, 886, 1231, 1351
BstYI RGATCY 4 cut(s) 364, 886, 1231, 1351
BsuI GTATCC 2 cut(s) 795, 2612
BtgI CCRYGG 2 cut(s) 2421, 2530
BtgZI GCGATG 1 cut(s) 1584
BtsIMutI CAGTG 5 cut(s) 608, 1000, 1035, 1741, 2893
BveI ACCTGC 1 cut(s) 2642
Cac8I GCNNGC 4 cut(s) 271, 987, 1118, 1777
CaiI CAGNNNCTG 1 cut(s) 1835
CfoI GCGC 2 cut(s) 288, 430
Cfr10I RCCGGY 2 cut(s) 1245, 1919
Cfr13I GGNCC 8 cut(s) 764, 911, 978, 1737, 1738, 2353, 2354, 2670
CsiI ACCWGGT 1 cut(s) 2794
Csp6I GTAC 8 cut(s) 502, 1206, 1210, 1520, 1832, 2203, 2943, 3007
CspAI ACCGGT 1 cut(s) 1245
CviQI GTAC 8 cut(s) 502, 1206, 1210, 1520, 1832, 2203, 2943, 3007
EaeI YGGCCR 3 cut(s) 118, 997, 1123
Eam1104I CTCTTC 4 cut(s) 801, 1285, 1816, 2708
EarI CTCTTC 4 cut(s) 801, 1285, 1816, 2708
EciI GGCGGA 1 cut(s) 2303
Ecl136II GAGCTC 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 1209
Eco130I CCWWGG 4 cut(s) 150, 678, 2421, 2530
Eco24I GRGCYC 4 cut(s) 90, 325, 1741, 2357
Eco31I GGTCTC 3 cut(s) 340, 1647, 2956
Eco32I GATATC 2 cut(s) 538, 1164
Eco47I GGWCC 3 cut(s) 764, 911, 978
Eco47III AGCGCT 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 962, 1866
Eco88I CYCGRG 2 cut(s) 2216, 2456
Eco91I GGTNACC 1 cut(s) 1040
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 3 cut(s) 978, 1737, 2353
EcoO65I GGTNACC 1 cut(s) 1040
EcoRI GAATTC 3 cut(s) 1391, 1484, 1567
EcoRII CCWGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
EcoRV GATATC 2 cut(s) 538, 1164
EcoT14I CCWWGG 4 cut(s) 150, 678, 2421, 2530
EcoT22I ATGCAT 2 cut(s) 1721, 2731
EcoT38I GRGCYC 4 cut(s) 90, 325, 1741, 2357
ErhI CCWWGG 4 cut(s) 150, 678, 2421, 2530
Esp3I CGTCTC 1 cut(s) 1498
FaqI GGGAC 3 cut(s) 1033, 1598, 1820
FauI CCCGC 1 cut(s) 2173
FauNDI CATATG 1 cut(s) 1864
FblI GTMKAC 3 cut(s) 1014, 1907, 2695
Fnu4HI GCNGC 4 cut(s) 51, 284, 1656, 2753
FriOI GRGCYC 4 cut(s) 90, 325, 1741, 2357
Fsp4HI GCNGC 4 cut(s) 51, 284, 1656, 2753
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 2 cut(s) 287, 429
GluI GCNGC 4 cut(s) 51, 284, 1656, 2753
GsuI CTGGAG 3 cut(s) 705, 2073, 2909
HaeII RGCGCY 1 cut(s) 431
HapII CCGG 3 cut(s) 842, 1246, 1920
HhaI GCGC 2 cut(s) 288, 430
Hin1I GRCGYC 1 cut(s) 2721
Hin6I GCGC 2 cut(s) 286, 428
HinP1I GCGC 2 cut(s) 286, 428
HindIII AAGCTT 2 cut(s) 1114, 1270
HpaII CCGG 3 cut(s) 842, 1246, 1920
HphI GGTGA 4 cut(s) 1934, 2117, 2436, 2833
Hpy166II GTNNAC 5 cut(s) 608, 1015, 1908, 2696, 3007
Hpy8I GTNNAC 5 cut(s) 608, 1015, 1908, 2696, 3007
HpyCH4III ACNGT 5 cut(s) 1004, 1745, 2846, 2888, 2980
HpyCH4IV ACGT 3 cut(s) 1208, 1491, 2721
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 427, 1122, 1706, 2141
HpySE526I ACGT 3 cut(s) 1208, 1491, 2721
Hsp92I GRCGYC 1 cut(s) 2721
HspAI GCGC 2 cut(s) 286, 428
LmnI GCTCC 6 cut(s) 2132, 2167, 2307, 2362, 2749, 2928
Lsp1109I GCAGC 4 cut(s) 62, 270, 1642, 2739
LweI GCATC 6 cut(s) 62, 64, 155, 730, 2319, 2963
MabI ACCWGGT 1 cut(s) 2794
MaeII ACGT 3 cut(s) 1208, 1491, 2721
MaeIII GTNAC 7 cut(s) 208, 626, 668, 1040, 1922, 2342, 2717
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 4 cut(s) 364, 886, 1231, 1351
MhlI GDGCHC 6 cut(s) 90, 325, 1741, 2164, 2357, 2659
MlsI TGGCCA 3 cut(s) 120, 999, 1125
MluNI TGGCCA 3 cut(s) 120, 999, 1125
MlyI GAGTC 4 cut(s) 1802, 1913, 2228, 2611
MmeI TCCRAC 2 cut(s) 1111, 2727
Mox20I TGGCCA 3 cut(s) 120, 999, 1125
Mph1103I ATGCAT 2 cut(s) 1721, 2731
MroXI GAANNNNTTC 2 cut(s) 1484, 2767
MscI TGGCCA 3 cut(s) 120, 999, 1125
Msp20I TGGCCA 3 cut(s) 120, 999, 1125
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 3 cut(s) 842, 1246, 1920
MspR9I CCNGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
MunI CAATTG 1 cut(s) 175
Mva1269I GAATGC 4 cut(s) 714, 2135, 2653, 2861
MvaI CCWGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
MwoI GCNNNNNNNGC 5 cut(s) 243, 427, 1122, 1706, 2141
NcoI CCATGG 2 cut(s) 2421, 2530
NdeI CATATG 1 cut(s) 1864
NlaIV GGNNCC 6 cut(s) 979, 1739, 2128, 2354, 2355, 2672
NmuCI GTSAC 4 cut(s) 208, 668, 1922, 2717
NsbI TGCGCA 1 cut(s) 287
NsiI ATGCAT 2 cut(s) 1721, 2731
NspV TTCGAA 1 cut(s) 2039
PaeR7I CTCGAG 2 cut(s) 2216, 2456
PaqCI CACCTGC 1 cut(s) 2642
PctI GAATGC 4 cut(s) 714, 2135, 2653, 2861
PdmI GAANNNNTTC 2 cut(s) 1484, 2767
PfeI GAWTC 9 cut(s) 100, 140, 343, 436, 1053, 1453, 1970, 2401, 2872
PinAI ACCGGT 1 cut(s) 1245
PkrI GCNGC 4 cut(s) 52, 285, 1657, 2754
PleI GAGTC 4 cut(s) 1802, 1912, 2227, 2611
PpsI GAGTC 4 cut(s) 1802, 1912, 2227, 2611
Ppu21I YACGTR 1 cut(s) 1209
PpuMI RGGWCCY 1 cut(s) 978
Psp124BI GAGCTC 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 978
Psp6I CCWGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
PspEI GGTNACC 1 cut(s) 1040
PspGI CCWGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
PspN4I GGNNCC 6 cut(s) 979, 1739, 2128, 2354, 2355, 2672
PspOMI GGGCCC 2 cut(s) 1737, 2353
PspPI GGNCC 8 cut(s) 764, 911, 978, 1737, 1738, 2353, 2354, 2670
PspPPI RGGWCCY 1 cut(s) 978
PspXI VCTCGAGB 1 cut(s) 2216
PstNI CAGNNNCTG 1 cut(s) 1835
PsuI RGATCY 4 cut(s) 364, 886, 1231, 1351
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 8 cut(s) 503, 1207, 1211, 1521, 1833, 2204, 2944, 3008
RsaNI GTAC 8 cut(s) 502, 1206, 1210, 1520, 1832, 2203, 2943, 3007
SacI GAGCTC 1 cut(s) 90
SatI GCNGC 4 cut(s) 51, 284, 1656, 2753
Sau96I GGNCC 8 cut(s) 764, 911, 978, 1737, 1738, 2353, 2354, 2670
SchI GAGTC 4 cut(s) 1802, 1913, 2228, 2611
ScrFI CCNGG 8 cut(s) 239, 1122, 1340, 1563, 1836, 2132, 2796, 2927
SduI GDGCHC 6 cut(s) 90, 325, 1741, 2164, 2357, 2659
SexAI ACCWGGT 1 cut(s) 2794
SfaNI GCATC 6 cut(s) 62, 64, 155, 730, 2319, 2963
SfcI CTRYAG 1 cut(s) 2478
Sfr274I CTCGAG 2 cut(s) 2216, 2456
SfuI TTCGAA 1 cut(s) 2039
SinI GGWCC 3 cut(s) 764, 911, 978
SlaI CTCGAG 2 cut(s) 2216, 2456
SmlI CTYRAG 3 cut(s) 2216, 2456, 2468
SmoI CTYRAG 3 cut(s) 2216, 2456, 2468
SnaBI TACGTA 1 cut(s) 1209
SsiI CCGC 3 cut(s) 1896, 2166, 2314
SspI AATATT 4 cut(s) 656, 1290, 2554, 2613
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 8 cut(s) 237, 1120, 1338, 1561, 1834, 2130, 2794, 2925
StyI CCWWGG 4 cut(s) 150, 678, 2421, 2530
TaaI ACNGT 5 cut(s) 1004, 1745, 2846, 2888, 2980
TaiI ACGT 3 cut(s) 1211, 1494, 2724
TaqI TCGA 4 cut(s) 514, 2039, 2217, 2457
TatI WGTACW 2 cut(s) 2202, 3006
TfiI GAWTC 9 cut(s) 100, 140, 343, 436, 1053, 1453, 1970, 2401, 2872
TscAI CASTG 5 cut(s) 615, 1007, 1042, 1748, 2893
TseFI GTSAC 4 cut(s) 208, 668, 1922, 2717
TseI GCWGC 4 cut(s) 50, 283, 1655, 2752
Tsp45I GTSAC 4 cut(s) 208, 668, 1922, 2717
TspGWI ACGGA 1 cut(s) 1046
TspRI CASTG 5 cut(s) 615, 1007, 1042, 1748, 2893
VpaK11BI GGWCC 3 cut(s) 764, 911, 978
XapI RAATTY 8 cut(s) 145, 1099, 1391, 1484, 1528, 1567, 2279, 2678
XbaI TCTAGA 4 cut(s) 352, 1348, 1628, 1939
XhoI CTCGAG 2 cut(s) 2216, 2456
XmiI GTMKAC 3 cut(s) 1014, 1907, 2695
XmnI GAANNNNTTC 2 cut(s) 1484, 2767
ZraI GACGTC 1 cut(s) 2722
Zsp2I ATGCAT 2 cut(s) 1721, 2731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.