Rroxscaffold_1G00031220
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006137
Physical Location & Seq
Reverse (-)
43891408 .. 43897486
6079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031220.1

Sequence Viewer

Length: 3066 bp
ATGCAGACAATATGCTTCATGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGGTAGTAATTGCAGCATCAGGGCAGTCAGATTTAGATGCACTCCTAGAGCTCAAAAAAGGGATTCAGAAAGACCCTTCTGGCCAAGTTCTTGTTTCATGGGATTCAAAGTCCTTGGCCTCTGATGGATGCCCCCTCAATTGGTTTGGGATAGTTTGTAGTGATGGCCTTGTCACATCGATTTCTATAAACGATATTGGCCTGGTTGGCGAGTTCAGTTTTTCAGCCATTGCTGGCCTTAAAGTGCTGCGCAATTTATCACTCTCAAACAACCAGCTCACAGGGGCTATCTCAAAGGTCGCTCTTATTCAGTCTCTAGAGCATTTAGATCTCTCACGCAATTTGTTTCATGGGTTAATACCATCTGGCTTAGCAGACTTCAAGCATTTAGCGCTTTTGAATCTGTCTTCAAACCAATTTGAAGGCATTTTCCCCTCTGGTTTTGGGAAACTTGAACAGCTGAGGTACATTGATGTTCGAGATAATGCCTTGTCTGGGGATATCATGACTTTTCTATCCCAGATGGGTAGCGTGGTTCATCTTGATCTAAGTAGCAATCTGTTTTCTGGTTCACTGGATTTGGAAATTGGTAACTCCTCTTTTGTTTCTTCTGTTCAATATTTGAATGTAAGTCACAATTCCTTGGCTGGAGAGCTTTTTCCTCACGATGGAATGCCATATTTTGATAGTTTGGAGGTGTTTGATGCTTCTGATAATCAGTTAGTTGGTCCTATACCTTCCTTCAATTTTGTTGTCTCTCTTCGGATACTTCGGCTCGGAAGCAACCAGCTATCAGGTTCTCTACCGGAAGCTCTGTTGCAAGGAAGCTCAATGCTCTTGTCTGAATTGGATCTTAGTCTTAATCATCTTGAAGGTCCAGTAGGAAGCATAACCTCTGCAACTCTGAAGAAGCTGAATATATCTTCAAATAAATTATCAGGGTCCTTGCCTGCCAATGTTGGCCACTGTGCCATCGTAGACCTCAGTAATAATATGCTCACTGGTAACCTGTCCCGAATCCGTAGTTGGGGAAATTACATGGAAATTATCCAACTAAGTTCAAATTCTTTGACAGGAAGCTTGCCTGGCCAAACATCCCAATTTTTGAGGCTAACTTCTTTTAAGATATCCAATAACTCATTAGAAGGTGTTCTTCCATCAGTATTGGGTACATACCCAGAACTAAAAGTGATAGATCTTAGCCTCAACCGGTTGCAAGGTTCTCTCCTTCCAAGCTTTTTCTCTTCAACAATATTGACTGACCTAAACTTGTCAGGCAACAATTTCTCTGGGTCCATACCTGTCCAGGAAATTACAATTGGTTCTGCTCAAAATTTGAGCCTGGTGTCTCTAGATCTGTCAAATAATTCATTGAGTGGTCATTTGCCCCAAGAATTCAGCAAGTTTTATAGCTTGGTATATCTCAACCTGTCTGGTAATAATTTTGAAGGTAGGATTCCTGAGAACCTTCCAGATGAACTGAAAGAATTCAACGTCTCTTTGAATAATCTTTCTGGTCTTGTACCTGAAAATTTGAGGCACTTCCCTGATTCAGCATTTTATCCAGGAAATTCTTTGCTGATGTTTCCTCATCCCCTATCATCACCAAACAATGTCCCAGATATGATCTCTAGAAATCACAGGTCTCCTATAAAAGCTGCTATTAGAATTGCCCTGATTGTAGGTGTGCTTGGTGGTGGTGCTATTGTAGCCCTCTCATGCATGATGATCTATTATAGGGCCCACTGTCAAGGATGTAGAAAGAGAGGTCATAAAGCAAGCTGTGAAAAGAATGGTGTTGTGCAAGGGGACTCTTCCCTTTCTCATAGATCAGTACCTGGCAAAACTGAAGCCTTAAAATCTTCATATGGTTTCCATCAAGACCCTTCGCCATCATCCACAAGGGAGTCTACTCGTAATACCAGTGACACTTCAGTTCTAGAGAAGTCTAAACAATTGGGTCATCCTGAATCAACAAAGCTAGAGGAAGGGGTATCTTCTCCCAAGTCTCTCTTATTGCCTTCAAACCCATTGCCTTCAAAAGGCCAACAACCACTCAATAGTTCTGCTGTTCTCAACACTTGTTCTCCAGATAAACTAGCTGGTGATTTACATCTGTTTGATGGCTCCCTGGCATTCACAGCAGAAGAACTTTCATGTGCTCCCGCAGAAGCTATTGGAAGGAGTTGTCATGGAACAATGTACAAAGCAATGCTCGCGTCTGGTCATGTAATTGCTGTCAAATGGTTAAGGGAGGGAATAGCAAAAGGAAGGAAAGAATTTGCTAGAGAAGTAAAGAAACTTGGGAGCATCCGCCATCCAAATCTAGTTTCTTTGCAGGGTTACTATTGGGGCCCAAAGGAGCATGAGAAACTTATTATATCAAATTATATCAATGCTGAATCTTTGGCCTTCTATCTCCATGAGGTTGAGCCAAGAAAACTCTCACCCTTGTCTCTCGAGGAACGGCTCAAGGTTTCTGTAGATGTTGCCCGATGTCTGAACTACCTCCATAATGAGAAGGCAATTCCTCATGGCAACCTCAAATCCACAAATATTCTACTAGAAACTCCTAATCATAATGCACTCCTTACAGATTATAGTATCCACCGAATATTGACTCCTGCTGGAACTACTGAGCAAGTTCTGAATGCAGGTGCTCTTGGCTATCGGCCCCCTGAATTTGCTAGTTCAAGTAGACCCTGCCCCTCATTGAAGAGTGATGTCTATGCATTTGGAGTGATCTTGTTGGAGCTCCTGACTGGAATGAGTTCTGGGGAGATTGTCTCTGGCATACCAGGTGTGGTTGATCTGACAGATTGGGTGAGATTTTTAGCAGAAGAAAACCGTTCTTTTGAATGCCTTGACAGACTGATTCTGGAAAAGCACAGTATTAAGCACTCACCTAGAGTTCTTGATGAATTGCTCCAGGTAGCTTTAAGATGTATCCTTCCAGCATCCGAGAGACCCGACATTAAAACTGTGTTTGAAGACCTTTCAAGAATAGTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1021

Amino Acids

110.83

Weight (kDa)

6.15

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 71 5e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 100 - 157 6e-06 Leucine rich repeat
LRR_8 PF13855 146 - 204 2e-07 Leucine rich repeat
LRR_8 PF13855 387 - 446 8.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 741 - 1015 1.8e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 741 - 940 1.4e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2696
Acc16I TGCGCA 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 2696
AccI GTMKAC 3 cut(s) 1029, 1961, 2749
AccII CGCG 1 cut(s) 2270
AciI CCGC 2 cut(s) 2217, 2365
AclWI GGATC 1 cut(s) 909
AcoI YGGCCR 3 cut(s) 133, 1012, 1138
AcsI RAATTY 8 cut(s) 1114, 1384, 1445, 1538, 1582, 1621, 2330, 2732
AcuI CTGAAG 3 cut(s) 977, 1920, 1968
AfaI GTAC 6 cut(s) 518, 1222, 1575, 1887, 2255, 3062
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 5 cut(s) 192, 546, 719, 1078, 2093
AgeI ACCGGT 1 cut(s) 1260
AjnI CCWGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
AjuI GAANNNNNNNTTGG 6 cut(s) 1060, 1092, 1353, 1385, 2365, 2397
Alw21I GWGCWC 4 cut(s) 105, 2215, 2713, 2808
Alw26I GTCTC 9 cut(s) 369, 811, 1404, 1552, 1701, 2064, 2511, 2842, 3010
AlwI GGATC 1 cut(s) 909
AlwNI CAGNNNCTG 1 cut(s) 1889
Ama87I CYCGRG 1 cut(s) 2510
Aor51HI AGCGCT 1 cut(s) 444
ApaI GGGCCC 2 cut(s) 1795, 2408
ApeKI GCWGC 3 cut(s) 65, 298, 1709
ApoI RAATTY 8 cut(s) 1114, 1384, 1445, 1538, 1582, 1621, 2330, 2732
AsiGI ACCGGT 1 cut(s) 1260
Asp700I GAANNNNTTC 3 cut(s) 1200, 1538, 2821
AspLEI GCGC 2 cut(s) 303, 445
AspS9I GGNCC 9 cut(s) 779, 926, 993, 1344, 1791, 1792, 2404, 2405, 2724
AsuHPI GGTGA 5 cut(s) 1647, 2168, 2490, 2887, 2946
AvaI CYCGRG 1 cut(s) 2510
AvaII GGWCC 4 cut(s) 779, 926, 993, 1344
BaeGI GKGCMC 2 cut(s) 1795, 2408
BalI TGGCCA 3 cut(s) 135, 1014, 1140
BanII GRGCYC 4 cut(s) 105, 1795, 2408, 2808
BbsI GAAGAC 2 cut(s) 450, 3048
Bbv12I GWGCWC 4 cut(s) 105, 2215, 2713, 2808
BbvCI CCTCAGC 1 cut(s) 512
BbvI GCAGC 3 cut(s) 77, 285, 1696
BceAI ACGGC 1 cut(s) 2534
BciT130I CCWGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
BciVI GTATCC 3 cut(s) 810, 2666, 3008
BcoDI GTCTC 9 cut(s) 369, 811, 1404, 1552, 1701, 2064, 2511, 2842, 3010
BfmI CTRYAG 1 cut(s) 2532
BfoI RGCGCY 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 2696
BfuI GTATCC 3 cut(s) 810, 2666, 3008
BglI GCCNNNNNGGC 1 cut(s) 258
BglII AGATCT 3 cut(s) 379, 1246, 1405
BisI GCNGC 3 cut(s) 66, 299, 1710
BlpI GCTNAGC 1 cut(s) 421
BlsI GCNGC 3 cut(s) 67, 300, 1711
Bme1390I CCNGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
Bme18I GGWCC 4 cut(s) 779, 926, 993, 1344
BmeT110I CYCGRG 1 cut(s) 2510
BmgT120I GGNCC 9 cut(s) 779, 926, 993, 1344, 1791, 1792, 2404, 2405, 2724
BmiI GGNNCC 7 cut(s) 994, 1345, 1793, 2179, 2405, 2406, 2726
BmrFI CCNGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
BmsI GCATC 6 cut(s) 77, 79, 170, 745, 2370, 3017
BpiI GAAGAC 2 cut(s) 450, 3048
BplI GAGNNNNNCTC 2 cut(s) 2822, 2854
BpmI CTGGAG 3 cut(s) 720, 2124, 2963
Bpu10I CCTNAGC 1 cut(s) 512
Bpu1102I GCTNAGC 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 2507
Bsa29I ATCGAT 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 2163
BsaI GGTCTC 2 cut(s) 1701, 3010
BsaJI CCNNGG 3 cut(s) 165, 693, 2181
BsaWI WCCGGW 2 cut(s) 856, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 2122, 2152
Bsc4I CCNNNNNNNGG 5 cut(s) 192, 546, 719, 1078, 2093
Bse118I RCCGGY 1 cut(s) 1260
Bse1I ACTGG 5 cut(s) 630, 929, 1057, 1974, 2818
Bse3DI GCAATG 3 cut(s) 279, 2081, 2268
Bse8I GATNNNNATC 1 cut(s) 2163
BseBI CCWGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
BseCI ATCGAT 1 cut(s) 230
BseDI CCNNGG 3 cut(s) 165, 693, 2181
BseGI GGATG 9 cut(s) 185, 1145, 1642, 1811, 1946, 2014, 2361, 2368, 3008
BseJI GATNNNNATC 1 cut(s) 2163
BseLI CCNNNNNNNGG 5 cut(s) 192, 546, 719, 1078, 2093
BseMI GCAATG 3 cut(s) 279, 2081, 2268
BseMII CTCAG 4 cut(s) 503, 1048, 1503, 2679
BseNI ACTGG 5 cut(s) 630, 929, 1057, 1974, 2818
BseRI GAGGAG 1 cut(s) 637
BseSI GKGCMC 2 cut(s) 1795, 2408
BseXI GCAGC 3 cut(s) 77, 285, 1696
Bsh1236I CGCG 1 cut(s) 2270
BshTI ACCGGT 1 cut(s) 1260
BshVI ATCGAT 1 cut(s) 230
BsiHKAI GWGCWC 4 cut(s) 105, 2215, 2713, 2808
BsiHKCI CYCGRG 1 cut(s) 2510
BsiSI CCGG 2 cut(s) 857, 1261
BslFI GGGAC 3 cut(s) 1048, 1652, 1874
BslI CCNNNNNNNGG 5 cut(s) 192, 546, 719, 1078, 2093
BsmAI GTCTC 9 cut(s) 369, 811, 1404, 1552, 1701, 2064, 2511, 2842, 3010
BsmBI CGTCTC 1 cut(s) 1552
BsmFI GGGAC 3 cut(s) 1048, 1652, 1874
BsmI GAATGC 4 cut(s) 729, 2186, 2707, 2915
Bso31I GGTCTC 2 cut(s) 1701, 3010
BsoBI CYCGRG 1 cut(s) 2510
Bsp120I GGGCCC 2 cut(s) 1791, 2404
Bsp1286I GDGCHC 6 cut(s) 105, 1795, 2215, 2408, 2713, 2808
Bsp1407I TGTACA 1 cut(s) 2253
Bsp1720I GCTNAGC 1 cut(s) 421
BspACI CCGC 2 cut(s) 2217, 2365
BspCNI CTCAG 4 cut(s) 504, 1047, 1504, 2680
BspDI ATCGAT 1 cut(s) 230
BspFNI CGCG 1 cut(s) 2270
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 7 cut(s) 994, 1345, 1793, 2179, 2405, 2406, 2726
BspMI ACCTGC 1 cut(s) 2696
BspPI GGATC 1 cut(s) 909
BspTNI GGTCTC 2 cut(s) 1701, 3010
BsrDI GCAATG 3 cut(s) 279, 2081, 2268
BsrFI RCCGGY 1 cut(s) 1260
BsrGI TGTACA 1 cut(s) 2253
BsrI ACTGG 5 cut(s) 630, 929, 1057, 1974, 2818
BssAI RCCGGY 1 cut(s) 1260
BssECI CCNNGG 3 cut(s) 165, 693, 2181
BssT1I CCWWGG 2 cut(s) 165, 693
Bst2UI CCWGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
Bst4CI ACNGT 5 cut(s) 1019, 1799, 2900, 2942, 3034
Bst6I CTCTTC 4 cut(s) 816, 1300, 1870, 2762
BstAUI TGTACA 1 cut(s) 2253
BstC8I GCNNGC 5 cut(s) 286, 1002, 1133, 1831, 2268
BstDEI CTNAG 9 cut(s) 421, 512, 599, 905, 1034, 1106, 1250, 1512, 2688
BstEII GGTNACC 1 cut(s) 1055
BstENI CCTNNNNNAGG 1 cut(s) 2091
BstF5I GGATG 9 cut(s) 185, 1145, 1642, 1811, 1946, 2014, 2361, 2368, 3008
BstFNI CGCG 1 cut(s) 2270
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 2 cut(s) 303, 445
BstMAI GTCTC 9 cut(s) 369, 811, 1404, 1552, 1701, 2064, 2511, 2842, 3010
BstMWI GCNNNNNNNGC 6 cut(s) 258, 442, 1137, 1760, 2192, 2267
BstNI CCWGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
BstPI GGTNACC 1 cut(s) 1055
BstSCI CCNGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
BstSFI CTRYAG 1 cut(s) 2532
BstSLI GKGCMC 2 cut(s) 1795, 2408
BstUI CGCG 1 cut(s) 2270
BstV1I GCAGC 3 cut(s) 77, 285, 1696
BstV2I GAAGAC 2 cut(s) 450, 3048
BstX2I RGATCY 4 cut(s) 379, 901, 1246, 1405
BstYI RGATCY 4 cut(s) 379, 901, 1246, 1405
Bsu15I ATCGAT 1 cut(s) 230
BsuI GTATCC 3 cut(s) 810, 2666, 3008
BsuTUI ATCGAT 1 cut(s) 230
BtsCI GGATG 9 cut(s) 185, 1145, 1642, 1811, 1946, 2014, 2361, 2368, 3008
BtsIMutI CAGTG 5 cut(s) 623, 1015, 1050, 1795, 1981
BveI ACCTGC 1 cut(s) 2696
Cac8I GCNNGC 5 cut(s) 286, 1002, 1133, 1831, 2268
CaiI CAGNNNCTG 1 cut(s) 1889
CciI TCATGA 1 cut(s) 555
CfoI GCGC 2 cut(s) 303, 445
Cfr10I RCCGGY 1 cut(s) 1260
Cfr13I GGNCC 9 cut(s) 779, 926, 993, 1344, 1791, 1792, 2404, 2405, 2724
ClaI ATCGAT 1 cut(s) 230
CseI GACGC 1 cut(s) 2259
CsiI ACCWGGT 1 cut(s) 2848
Csp6I GTAC 6 cut(s) 517, 1221, 1574, 1886, 2254, 3061
CspAI ACCGGT 1 cut(s) 1260
CviQI GTAC 6 cut(s) 517, 1221, 1574, 1886, 2254, 3061
DdeI CTNAG 9 cut(s) 421, 512, 599, 905, 1034, 1106, 1250, 1512, 2688
EaeI YGGCCR 3 cut(s) 133, 1012, 1138
Eam1104I CTCTTC 4 cut(s) 816, 1300, 1870, 2762
EarI CTCTTC 4 cut(s) 816, 1300, 1870, 2762
EciI GGCGGA 1 cut(s) 2354
Ecl136II GAGCTC 2 cut(s) 103, 2806
Eco130I CCWWGG 2 cut(s) 165, 693
Eco24I GRGCYC 4 cut(s) 105, 1795, 2408, 2808
Eco31I GGTCTC 2 cut(s) 1701, 3010
Eco32I GATATC 2 cut(s) 553, 1179
Eco47I GGWCC 4 cut(s) 779, 926, 993, 1344
Eco47III AGCGCT 1 cut(s) 444
Eco53kI GAGCTC 2 cut(s) 103, 2806
Eco57I CTGAAG 3 cut(s) 977, 1920, 1968
Eco88I CYCGRG 1 cut(s) 2510
Eco91I GGTNACC 1 cut(s) 1055
EcoICRI GAGCTC 2 cut(s) 103, 2806
EcoNI CCTNNNNNAGG 1 cut(s) 2091
EcoO109I RGGNCCY 3 cut(s) 993, 1791, 2404
EcoO65I GGTNACC 1 cut(s) 1055
EcoRI GAATTC 2 cut(s) 1445, 1538
EcoRII CCWGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
EcoRV GATATC 2 cut(s) 553, 1179
EcoT14I CCWWGG 2 cut(s) 165, 693
EcoT22I ATGCAT 2 cut(s) 1775, 2785
EcoT38I GRGCYC 4 cut(s) 105, 1795, 2408, 2808
ErhI CCWWGG 2 cut(s) 165, 693
Esp3I CGTCTC 1 cut(s) 1552
FalI AAGNNNNNCTT 4 cut(s) 1188, 1220, 2048, 2080
FaqI GGGAC 3 cut(s) 1048, 1652, 1874
FauI CCCGC 1 cut(s) 2224
FauNDI CATATG 1 cut(s) 1918
FblI GTMKAC 3 cut(s) 1029, 1961, 2749
Fnu4HI GCNGC 3 cut(s) 66, 299, 1710
FokI GGATG 9 cut(s) 192, 1132, 1629, 1818, 1933, 2001, 2348, 2355, 2995
FriOI GRGCYC 4 cut(s) 105, 1795, 2408, 2808
Fsp4HI GCNGC 3 cut(s) 66, 299, 1710
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 2 cut(s) 302, 444
GluI GCNGC 3 cut(s) 66, 299, 1710
GsuI CTGGAG 3 cut(s) 720, 2124, 2963
HaeII RGCGCY 1 cut(s) 446
HapII CCGG 2 cut(s) 857, 1261
HgaI GACGC 1 cut(s) 2259
HhaI GCGC 2 cut(s) 303, 445
Hin6I GCGC 2 cut(s) 301, 443
HinP1I GCGC 2 cut(s) 301, 443
HindIII AAGCTT 2 cut(s) 1129, 1285
HpaII CCGG 2 cut(s) 857, 1261
HphI GGTGA 5 cut(s) 1647, 2168, 2490, 2887, 2946
Hpy166II GTNNAC 5 cut(s) 623, 1030, 1962, 2750, 3061
Hpy8I GTNNAC 5 cut(s) 623, 1030, 1962, 2750, 3061
HpyCH4III ACNGT 5 cut(s) 1019, 1799, 2900, 2942, 3034
HpyCH4IV ACGT 1 cut(s) 1545
HpyF10VI GCNNNNNNNGC 6 cut(s) 258, 442, 1137, 1760, 2192, 2267
HpyF3I CTNAG 9 cut(s) 421, 512, 599, 905, 1034, 1106, 1250, 1512, 2688
HpySE526I ACGT 1 cut(s) 1545
HspAI GCGC 2 cut(s) 301, 443
LmnI GCTCC 7 cut(s) 2183, 2218, 2358, 2413, 2803, 2811, 2982
Lsp1109I GCAGC 3 cut(s) 77, 285, 1696
LweI GCATC 6 cut(s) 77, 79, 170, 745, 2370, 3017
MabI ACCWGGT 1 cut(s) 2848
MaeII ACGT 1 cut(s) 1545
MaeIII GTNAC 6 cut(s) 223, 641, 683, 1055, 1976, 2393
MfeI CAATTG 3 cut(s) 190, 1368, 2006
MflI RGATCY 4 cut(s) 379, 901, 1246, 1405
MhlI GDGCHC 6 cut(s) 105, 1795, 2215, 2408, 2713, 2808
MlsI TGGCCA 3 cut(s) 135, 1014, 1140
MluNI TGGCCA 3 cut(s) 135, 1014, 1140
MlyI GAGTC 3 cut(s) 1856, 1967, 2665
MmeI TCCRAC 2 cut(s) 1126, 2781
Mox20I TGGCCA 3 cut(s) 135, 1014, 1140
Mph1103I ATGCAT 2 cut(s) 1775, 2785
MroXI GAANNNNTTC 3 cut(s) 1200, 1538, 2821
MscI TGGCCA 3 cut(s) 135, 1014, 1140
MseI TTAA 9 cut(s) 291, 407, 912, 1173, 1907, 2300, 2946, 2990, 3027
Msp20I TGGCCA 3 cut(s) 135, 1014, 1140
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 2 cut(s) 857, 1261
MspR9I CCNGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
MunI CAATTG 3 cut(s) 190, 1368, 2006
Mva1269I GAATGC 4 cut(s) 729, 2186, 2707, 2915
MvaI CCWGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
MvnI CGCG 1 cut(s) 2270
MwoI GCNNNNNNNGC 6 cut(s) 258, 442, 1137, 1760, 2192, 2267
NdeI CATATG 1 cut(s) 1918
NlaIV GGNNCC 7 cut(s) 994, 1345, 1793, 2179, 2405, 2406, 2726
NmuCI GTSAC 3 cut(s) 223, 683, 1976
NsbI TGCGCA 1 cut(s) 302
NsiI ATGCAT 2 cut(s) 1775, 2785
PaeR7I CTCGAG 1 cut(s) 2510
PagI TCATGA 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 2696
PctI GAATGC 4 cut(s) 729, 2186, 2707, 2915
PdmI GAANNNNTTC 3 cut(s) 1200, 1538, 2821
PfeI GAWTC 9 cut(s) 115, 155, 451, 1068, 1507, 1601, 2021, 2453, 2926
PfoI TCCNGGA 2 cut(s) 1356, 1615
PinAI ACCGGT 1 cut(s) 1260
PkrI GCNGC 3 cut(s) 67, 300, 1711
PleI GAGTC 3 cut(s) 1856, 1966, 2665
PpsI GAGTC 3 cut(s) 1856, 1966, 2665
PpuMI RGGWCCY 1 cut(s) 993
Psp124BI GAGCTC 2 cut(s) 105, 2808
Psp5II RGGWCCY 1 cut(s) 993
Psp6I CCWGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
PspEI GGTNACC 1 cut(s) 1055
PspGI CCWGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
PspN4I GGNNCC 7 cut(s) 994, 1345, 1793, 2179, 2405, 2406, 2726
PspOMI GGGCCC 2 cut(s) 1791, 2404
PspPI GGNCC 9 cut(s) 779, 926, 993, 1344, 1791, 1792, 2404, 2405, 2724
PspPPI RGGWCCY 1 cut(s) 993
PstNI CAGNNNCTG 1 cut(s) 1889
PsuI RGATCY 4 cut(s) 379, 901, 1246, 1405
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 6 cut(s) 518, 1222, 1575, 1887, 2255, 3062
RsaNI GTAC 6 cut(s) 517, 1221, 1574, 1886, 2254, 3061
SacI GAGCTC 2 cut(s) 105, 2808
SaqAI TTAA 9 cut(s) 291, 407, 912, 1173, 1907, 2300, 2946, 2990, 3027
SatI GCNGC 3 cut(s) 66, 299, 1710
Sau96I GGNCC 9 cut(s) 779, 926, 993, 1344, 1791, 1792, 2404, 2405, 2724
SchI GAGTC 3 cut(s) 1856, 1967, 2665
ScrFI CCNGG 9 cut(s) 254, 1137, 1358, 1394, 1617, 1890, 2183, 2850, 2981
SduI GDGCHC 6 cut(s) 105, 1795, 2215, 2408, 2713, 2808
SexAI ACCWGGT 1 cut(s) 2848
SfaNI GCATC 6 cut(s) 77, 79, 170, 745, 2370, 3017
SfcI CTRYAG 1 cut(s) 2532
Sfr274I CTCGAG 1 cut(s) 2510
SinI GGWCC 4 cut(s) 779, 926, 993, 1344
SlaI CTCGAG 1 cut(s) 2510
SmlI CTYRAG 2 cut(s) 2510, 2522
SmoI CTYRAG 2 cut(s) 2510, 2522
SsiI CCGC 2 cut(s) 2217, 2365
SspI AATATT 4 cut(s) 671, 1305, 2608, 2667
SstI GAGCTC 2 cut(s) 105, 2808
StyD4I CCNGG 9 cut(s) 252, 1135, 1356, 1392, 1615, 1888, 2181, 2848, 2979
StyI CCWWGG 2 cut(s) 165, 693
TaaI ACNGT 5 cut(s) 1019, 1799, 2900, 2942, 3034
TaiI ACGT 1 cut(s) 1548
TaqI TCGA 3 cut(s) 230, 529, 2511
TatI WGTACW 2 cut(s) 2253, 3060
TfiI GAWTC 9 cut(s) 115, 155, 451, 1068, 1507, 1601, 2021, 2453, 2926
Tru1I TTAA 9 cut(s) 291, 407, 912, 1173, 1907, 2300, 2946, 2990, 3027
Tru9I TTAA 9 cut(s) 291, 407, 912, 1173, 1907, 2300, 2946, 2990, 3027
TscAI CASTG 5 cut(s) 630, 1022, 1057, 1802, 1981
TseFI GTSAC 3 cut(s) 223, 683, 1976
TseI GCWGC 3 cut(s) 65, 298, 1709
Tsp45I GTSAC 3 cut(s) 223, 683, 1976
TspDTI ATGAA 9 cut(s) 7, 138, 389, 578, 1410, 1542, 1905, 2196, 2985
TspGWI ACGGA 1 cut(s) 1061
TspRI CASTG 5 cut(s) 630, 1022, 1057, 1802, 1981
VpaK11BI GGWCC 4 cut(s) 779, 926, 993, 1344
XagI CCTNNNNNAGG 1 cut(s) 2091
XapI RAATTY 8 cut(s) 1114, 1384, 1445, 1538, 1582, 1621, 2330, 2732
XbaI TCTAGA 4 cut(s) 367, 1402, 1682, 1990
XhoI CTCGAG 1 cut(s) 2510
XmiI GTMKAC 3 cut(s) 1029, 1961, 2749
XmnI GAANNNNTTC 3 cut(s) 1200, 1538, 2821
Zsp2I ATGCAT 2 cut(s) 1775, 2785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.