MD11G1251700.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010431
Physical Location & Seq
Forward (+)
36369620 .. 36373784
4165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1251700.v1.1.491

Sequence Viewer

Length: 3162 bp
ATGGAGGTATTGGGGTTTACTCCAATGGCATTCCTCAAATGGGTTCTCTTGGCCTGCTGTATTGGTTCAGTTTTCGGTCTTAATTCCCCAACCCAATCCTGTTATCCAAATGATTTTCTTGCATTGAGGGAGTTTGCAGGAAACCTCACAAATGGGTCTATGATCACAGCATGGCACAACACATCAACTTGCTGCCAATGGGATGGTGTTGTCTGTGGGAATGTGAACAATGGGACAGTTGTTGCTAGTAGAGTCACACAGTTGATTCTTCCCAGCATGAGTCTCAGTGGAACGATTTCACGCTCTTTGGGTCGATTGGATCAGCTGAAGTTGCTTAATCTTTCTCTGAATCATCTTGAAGGTGGATTGCCTGCAGAGCTCTCAGACTTGAAGCACCTGGAGGTTCTTGACTTGAGCAATAACATGCTGTCCGGACCAGTTTCAGGTGCGCTTTCCGGTTTGAATTCGATCAGAGTTCTGAATATTTCAAGCAATTCAATCCATGATGATTTGTCTGAGCTTGGAGGAGGATTCTCACATCTTGTTGTGTTCAACATAAGCAATAATTCATTCACTGGTCAGTTCAACCCTCGGATTTGTAGCTCTTCCAATGAAACCCACATTCTAGATATGTCTTGGAACCGTTTGGTGGGAAGTCTTGAAGGCTTAGACAATTGCAGCAGGTCTCTCCAACAATTGCACCTGGACTTCAATTCATTTTCAGGCTATCTACCTGAATCTTTGTATACGTATTCGGCTTTGGAGCAGCTTTCAGTCTCTGGGAACTCTCTTTCCGGCCAGATAAGCAAGGAACTGAGTAAGCTTTCTAGCCTCAAGACCTTAGTCATTTCTTCAAACCAATTTTTGGGTGAACTTCCAAATGTGTTTGGGAACCTTAGACGACTAGAACAGTTAGTTGCACATTCAAATATGTTGTCTGGCCCATTAGCTTCAACCTTGGCACTCTGTTCAAATCTTCGAGTGCTTGACCTTCGAAACAATTCTCTATCCGGTTCCATTGATCTAAATTTCACTGGACTTCCAAAACTCTGCACACTTGATCTTGCCACTAATCATTTTTCTGGTTTCCTTCCAAATTCACTTTCTAATTGTCGTGAGTTGAAAACGTTAAGCCTTGCTAGGAATAAATTAAGGGGTTCAATTCCGGAAGACTTTGCCAAACTCACATCCCTATTCTTCCTCTCATTGTCGAATAACAGTTTTGTGAACTTATCTGAGACACTATCTGTGCTGCAGCAATGCAAAAATCTCACAACTCTAATTCTTTCAAAAAATTTCTTTGGTGAGGAGATTCCGAAAAATGCAAGTGGGTTTGAAAGCTTAATGGTTTTAGCACTCGGGAATTGTGCTCTCAAAGGCCAAATTCCAGTTTGGTTATTGAGTTGCAGGAAGTTGCAAGTCCTTGATTTATCTTGGAATCGCTTGGATGGTCCCATCCCTTTGTGGATAGGTCAGATGGAAAACTTGTTTTATTTGGACTTTTCAAATAATTCACTCTCGGGAGAAATCCCAAAAAGTATGACAGAGCTGAAAAGCCTCATATCTACAAATTGCACTCATGCAAACCTTATTGCTTCTGCTGGCATCCCGTTATTTGTAAAACGGAATAGGAGTGCTACTGGCCTGCAGTACAACCAGGTCTCAAATTTTCCTCCGTCAATACTCTTGAGCAATAACAGAATAAATGGAGCAATGTGGCCTGAGATCGGACGATTGAAGCAGCTCCATGTTTTGGATTGGAGCCGGAACAACATTAGTGGGACCATCCCAAGCTCCATCTCGGAGATGGAGAACTTGGAAGCATTGGATTTATCATTCAATGATCTCTATGGTTCGATCCCTCCATCACTTAGCAAGCTCACATTCTTGTCAAAGTTTAGCGTGGCAAATAATCACCTCCATGGAGTGATTCCCAATGGAGGACAGTTCTTAAGCTTTCCCAGCTCAAGTTTTGACGGTAACTCGGGGCTTTGTGGGGCAATGTACACTCCATGTGGTGATATCAGCAGTACAAGTCTCAAGCCTGTAACTCCATCCAGTTCAAACAGTAGATTTGGTCGAAACAGCATCCTTTGTGTAGTAATCAGCATAGGAGTTGGGGTTGCATTGCTTCTTGCAGTTGGTTTGCTTAAAATGTCAAGGAGGGGAGCAAAGGATCAAATTGATGATTTTGATGAGGACAGCAGGCCGCACAGGATATCCGGAGCCCTTGCATCGTCAAAACTGGTGCTGTTCCAAAATTCAGACTGTAAGGATTTTACAGTGTCAGACTTGTTAAAGTCTACAAACAATTTCAACCAAGCCAACATAATTGGTTGTGGTGGTTATGGACTGGTTTACAAAGCCAACCTACCGAATGGAGCAAAAGCTGCAATCAAGAGACTTTCTGGGGATTGTGGACAGATGGAACGTGAATTTCAAGCTGAAGTGGAAGCCCTCTCAAGAGCTCAGCACAAGAACCTTGTCTCTCTTCAAGGTTACTGCCGGCATGGTAATGACAGACTGTTAATCTACTCCTACATGGAGAACGGAAGCTTGGATTATTGGCTGCATGAAAGTGTTGATGGTGTTTCGCTTCTAAAATGGGATGTAAGACTTAAGATAGCTCAAGGTGCGGCTCGTGGGCTAGCCTACTTGCACAAGGGTTGTCAGCCAAATATAGTGCATCGAGATATAAAAACAAGCAACATTCTTCTAGATGAAAAATTTGAAGCTCACTTGGCTGATTTTGGTCTTTCAAGGTTACTTCGTCCTTACGATACCCATGTTACAACAGATCTGGTTGGGACTTTGGGTTATATTCCTCCAGAGTACAGTCAGACATTAACAGCAACCTGCAGGGGCGATGTTTACAGTTTTGGTGTTGTTCTTCTTGAGCTTCTTACTGGTAGGAGGCCTGTAGAAGTATGCAGAGGAAAAAACTGCAGAGATTTGGTATCGTGGATGTTTCAGATGAGATTTGAGAAGAGAGATGAAGAGATTATAGATTCGTCAATTTGGAATAAGAATCATGAGAAGCAACTTTTAGATGTTCTTGGTGTTGCCTGTAAATGCCTGGATCCCAATCCGAGGCAGAGGCCCTTTATTGAAGAAGTTGTGTCATGCCTTGACGGTATAGGATTTGAGAGCGGGAAACAATGA

Protein Analysis

1054

Amino Acids

115.36

Weight (kDa)

6.74

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 72 2.7e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 108 - 167 8.2e-07 Leucine rich repeat
LRR_8 PF13855 228 - 288 7.6e-06 Leucine rich repeat
LRR_14 PF23598 319 - 426 3.9e-06 Leucine-rich repeat region
LRR_8 PF13855 324 - 384 1.1e-08 Leucine rich repeat
LRR_8 PF13855 367 - 407 5.4e-06 Leucine rich repeat
Pkinase PF00069 773 - 1040 7.2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 775 - 1043 1.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 672, 2864
AccB7I CCANNNNNTGG 2 cut(s) 865, 1753
AccBSI CCGCTC 1 cut(s) 3150
AccI GTMKAC 2 cut(s) 746, 2303
AccIII TCCGGA 3 cut(s) 431, 1165, 2222
AciI CCGC 3 cut(s) 2210, 2636, 3150
AclI AACGTT 1 cut(s) 1127
AclWI GGATC 5 cut(s) 327, 1852, 2184, 3074, 3087
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 9 cut(s) 463, 1027, 1096, 1294, 1383, 1666, 2260, 2435, 2726
AcuI CTGAAG 2 cut(s) 347, 2466
AfaI GTAC 4 cut(s) 1652, 2006, 2032, 2834
AfiI CCNNNNNNNGG 8 cut(s) 40, 443, 649, 865, 1727, 1753, 1940, 3090
AflII CTTAAG 2 cut(s) 1951, 2618
AjnI CCWGG 4 cut(s) 396, 702, 1656, 3075
AjuI GAANNNNNNNTTGG 4 cut(s) 1087, 1119, 2540, 2572
AloI GAACNNNNNNTCC 2 cut(s) 776, 808
Alw21I GWGCWC 3 cut(s) 381, 1372, 2470
Alw26I GTCTC 8 cut(s) 287, 690, 781, 1232, 1666, 2042, 2395, 2491
AlwI GGATC 5 cut(s) 327, 1852, 2184, 3074, 3087
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 3 cut(s) 1358, 1519, 1984
Aor13HI TCCGGA 3 cut(s) 431, 1165, 2222
AoxI GGCC 9 cut(s) 51, 796, 940, 1378, 1642, 1718, 2207, 2915, 3098
ApeKI GCWGC 8 cut(s) 192, 678, 766, 1252, 1255, 1741, 2390, 2569
ApoI RAATTY 9 cut(s) 463, 1027, 1096, 1294, 1383, 1666, 2260, 2435, 2726
ArsI GACNNNNNNTTYG 2 cut(s) 920, 952
Asp700I GAANNNNTTC 2 cut(s) 295, 1000
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 5 cut(s) 434, 941, 1451, 1782, 3099
AsuHPI GGTGA 4 cut(s) 881, 1316, 1907, 2030
AsuII TTCGAA 1 cut(s) 994
AsuNHI GCTAGC 1 cut(s) 2647
AvaI CYCGRG 3 cut(s) 1358, 1519, 1984
AvaII GGWCC 3 cut(s) 434, 1451, 1782
BaeI ACNNNNGTAYC 2 cut(s) 2772, 2805
BamHI GGATCC 1 cut(s) 3079
BanII GRGCYC 3 cut(s) 381, 2230, 2470
BauI CACGAG 1 cut(s) 2640
BbsI GAAGAC 1 cut(s) 1176
Bbv12I GWGCWC 3 cut(s) 381, 1372, 2470
BbvI GCAGC 8 cut(s) 179, 690, 778, 1239, 1267, 1753, 2377, 2556
BciT130I CCWGG 4 cut(s) 398, 704, 1658, 3077
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 8 cut(s) 287, 690, 781, 1232, 1666, 2042, 2395, 2491
BfaI CTAG 7 cut(s) 246, 626, 828, 905, 1140, 2648, 2717
BfmI CTRYAG 6 cut(s) 372, 1253, 1646, 2857, 2919, 2944
BfrI CTTAAG 2 cut(s) 1951, 2618
BfuAI ACCTGC 2 cut(s) 672, 2864
BglII AGATCT 1 cut(s) 2797
BlpI GCTNAGC 1 cut(s) 2469
Bme1390I CCNGG 4 cut(s) 398, 704, 1658, 3077
Bme18I GGWCC 3 cut(s) 434, 1451, 1782
BmeT110I CYCGRG 3 cut(s) 1358, 1519, 1984
BmgT120I GGNCC 5 cut(s) 434, 941, 1451, 1782, 3099
BmiI GGNNCC 8 cut(s) 641, 893, 1015, 1453, 1763, 1783, 2227, 3081
BmrFI CCNGG 4 cut(s) 398, 704, 1658, 3077
BmsI GCATC 4 cut(s) 1614, 2097, 2243, 2695
BmtI GCTAGC 1 cut(s) 2651
BoxI GACNNNNGTC 1 cut(s) 842
BpiI GAAGAC 1 cut(s) 1176
BpmI CTGGAG 2 cut(s) 419, 2811
Bpu1102I GCTNAGC 1 cut(s) 2469
Bpu14I TTCGAA 1 cut(s) 994
BpuEI CTTGAG 8 cut(s) 433, 818, 1708, 1951, 2024, 2446, 2613, 2915
BsaAI YACGTR 1 cut(s) 750
BsaBI GATNNNNATC 1 cut(s) 3084
BsaI GGTCTC 2 cut(s) 690, 1666
BsaJI CCNNGG 4 cut(s) 590, 957, 1921, 3089
BsaWI WCCGGW 5 cut(s) 431, 455, 1010, 1165, 2222
BsaXI ACNNNNNCTCC 2 cut(s) 2106, 2136
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 443, 649, 865, 1727, 1753, 1940, 3090
Bse118I RCCGGY 1 cut(s) 2505
Bse1I ACTGG 9 cut(s) 437, 580, 1039, 1388, 1645, 2058, 2250, 2358, 2911
Bse3DI GCAATG 4 cut(s) 1265, 1719, 2007, 2126
Bse8I GATNNNNATC 1 cut(s) 3084
BseAI TCCGGA 3 cut(s) 431, 1165, 2222
BseBI CCWGG 4 cut(s) 398, 704, 1658, 3077
BseDI CCNNGG 4 cut(s) 590, 957, 1921, 3089
BseJI GATNNNNATC 1 cut(s) 3084
BseLI CCNNNNNNNGG 8 cut(s) 40, 443, 649, 865, 1727, 1753, 1940, 3090
BseMI GCAATG 4 cut(s) 1265, 1719, 2007, 2126
BseMII CTCAG 7 cut(s) 298, 396, 507, 806, 1227, 1713, 2483
BseNI ACTGG 9 cut(s) 437, 580, 1039, 1388, 1645, 2058, 2250, 2358, 2911
BseRI GAGGAG 2 cut(s) 540, 1322
BseXI GCAGC 8 cut(s) 179, 690, 778, 1239, 1267, 1753, 2377, 2556
BseYI CCCAGC 2 cut(s) 272, 1961
BsgI GTGCAG 1 cut(s) 1036
BshFI GGCC 9 cut(s) 53, 798, 942, 1380, 1644, 1720, 2209, 2917, 3100
BsiHKAI GWGCWC 3 cut(s) 381, 1372, 2470
BsiHKCI CYCGRG 3 cut(s) 1358, 1519, 1984
BsiSI CCGG 8 cut(s) 432, 456, 795, 1011, 1166, 1765, 2223, 2506
BslFI GGGAC 4 cut(s) 247, 1437, 1795, 2821
BslI CCNNNNNNNGG 8 cut(s) 40, 443, 649, 865, 1727, 1753, 1940, 3090
BsmAI GTCTC 8 cut(s) 287, 690, 781, 1232, 1666, 2042, 2395, 2491
BsmFI GGGAC 4 cut(s) 247, 1437, 1795, 2821
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 9 cut(s) 53, 798, 942, 1380, 1644, 1720, 2209, 2917, 3100
Bso31I GGTCTC 2 cut(s) 690, 1666
BsoBI CYCGRG 3 cut(s) 1358, 1519, 1984
Bsp119I TTCGAA 1 cut(s) 994
Bsp1286I GDGCHC 4 cut(s) 381, 1372, 2230, 2470
Bsp13I TCCGGA 3 cut(s) 431, 1165, 2222
Bsp1407I TGTACA 1 cut(s) 2004
Bsp1720I GCTNAGC 1 cut(s) 2469
Bsp19I CCATGG 1 cut(s) 1921
BspACI CCGC 3 cut(s) 2210, 2636, 3150
BspANI GGCC 9 cut(s) 53, 798, 942, 1380, 1644, 1720, 2209, 2917, 3100
BspCNI CTCAG 7 cut(s) 297, 395, 508, 807, 1228, 1714, 2482
BspEI TCCGGA 3 cut(s) 431, 1165, 2222
BspHI TCATGA 1 cut(s) 3031
BspLI GGNNCC 8 cut(s) 641, 893, 1015, 1453, 1763, 1783, 2227, 3081
BspMAI CTGCAG 5 cut(s) 376, 1257, 1650, 2861, 2948
BspMI ACCTGC 2 cut(s) 672, 2864
BspOI GCTAGC 1 cut(s) 2651
BspPI GGATC 5 cut(s) 327, 1852, 2184, 3074, 3087
BspQI GCTCTTC 1 cut(s) 610
BspT104I TTCGAA 1 cut(s) 994
BspTI CTTAAG 2 cut(s) 1951, 2618
BspTNI GGTCTC 2 cut(s) 690, 1666
BsrBI CCGCTC 1 cut(s) 3150
BsrDI GCAATG 4 cut(s) 1265, 1719, 2007, 2126
BsrFI RCCGGY 1 cut(s) 2505
BsrGI TGTACA 1 cut(s) 2004
BsrI ACTGG 9 cut(s) 437, 580, 1039, 1388, 1645, 2058, 2250, 2358, 2911
BssAI RCCGGY 1 cut(s) 2505
BssECI CCNNGG 4 cut(s) 590, 957, 1921, 3089
BssNAI GTATAC 1 cut(s) 747
BssSI CACGAG 1 cut(s) 2640
BssT1I CCWWGG 2 cut(s) 957, 1921
Bst1107I GTATAC 1 cut(s) 747
Bst2BI CACGAG 1 cut(s) 2640
Bst2UI CCWGG 4 cut(s) 398, 704, 1658, 3077
Bst6I CTCTTC 4 cut(s) 610, 2496, 2981, 2991
BstAFI CTTAAG 2 cut(s) 1951, 2618
BstAPI GCANNNNNTGC 1 cut(s) 2390
BstAUI TGTACA 1 cut(s) 2004
BstBAI YACGTR 1 cut(s) 750
BstBI TTCGAA 1 cut(s) 994
BstC8I GCNNGC 8 cut(s) 55, 372, 1603, 1646, 1877, 2207, 2507, 2649
BstDSI CCRYGG 1 cut(s) 1921
BstHHI GCGC 1 cut(s) 451
BstMAI GTCTC 8 cut(s) 287, 690, 781, 1232, 1666, 2042, 2395, 2491
BstMWI GCNNNNNNNGC 7 cut(s) 331, 376, 804, 1962, 2390, 2633, 2741
BstNI CCWGG 4 cut(s) 398, 704, 1658, 3077
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 842
BstSCI CCNGG 4 cut(s) 396, 702, 1656, 3075
BstSFI CTRYAG 6 cut(s) 372, 1253, 1646, 2857, 2919, 2944
BstSNI TACGTA 1 cut(s) 750
BstV1I GCAGC 8 cut(s) 179, 690, 778, 1239, 1267, 1753, 2377, 2556
BstV2I GAAGAC 1 cut(s) 1176
BstX2I RGATCY 2 cut(s) 2797, 3079
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 2 cut(s) 2797, 3079
BstZ17I GTATAC 1 cut(s) 747
BsuRI GGCC 9 cut(s) 53, 798, 942, 1380, 1644, 1720, 2209, 2917, 3100
BtgI CCRYGG 1 cut(s) 1921
BtgZI GCGATG 1 cut(s) 2880
BtsIMutI CAGTG 4 cut(s) 292, 573, 1032, 2289
BveI ACCTGC 2 cut(s) 672, 2864
Cac8I GCNNGC 8 cut(s) 55, 372, 1603, 1646, 1877, 2207, 2507, 2649
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 3031
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 1 cut(s) 2505
Cfr13I GGNCC 5 cut(s) 434, 941, 1451, 1782, 3099
CsiI ACCWGGT 1 cut(s) 1656
Csp6I GTAC 4 cut(s) 1651, 2005, 2031, 2833
CspCI CAANNNNNGTGG 2 cut(s) 1759, 1794
CviQI GTAC 4 cut(s) 1651, 2005, 2031, 2833
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 4 cut(s) 610, 2496, 2981, 2991
EarI CTCTTC 4 cut(s) 610, 2496, 2981, 2991
Ecl136II GAGCTC 2 cut(s) 379, 2468
Eco105I TACGTA 1 cut(s) 750
Eco130I CCWWGG 2 cut(s) 957, 1921
Eco147I AGGCCT 1 cut(s) 2917
Eco24I GRGCYC 3 cut(s) 381, 2230, 2470
Eco31I GGTCTC 2 cut(s) 690, 1666
Eco32I GATATC 2 cut(s) 2023, 2220
Eco47I GGWCC 3 cut(s) 434, 1451, 1782
Eco53kI GAGCTC 2 cut(s) 379, 2468
Eco57I CTGAAG 2 cut(s) 347, 2466
Eco88I CYCGRG 3 cut(s) 1358, 1519, 1984
EcoICRI GAGCTC 2 cut(s) 379, 2468
EcoO109I RGGNCCY 1 cut(s) 3099
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 4 cut(s) 396, 702, 1656, 3075
EcoRV GATATC 2 cut(s) 2023, 2220
EcoT14I CCWWGG 2 cut(s) 957, 1921
EcoT38I GRGCYC 3 cut(s) 381, 2230, 2470
ErhI CCWWGG 2 cut(s) 957, 1921
FaqI GGGAC 4 cut(s) 247, 1437, 1795, 2821
FauI CCCGC 1 cut(s) 3143
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 2 cut(s) 746, 2303
FriOI GRGCYC 3 cut(s) 381, 2230, 2470
FspBI CTAG 7 cut(s) 246, 626, 828, 905, 1140, 2648, 2717
GlaI GCGC 1 cut(s) 450
GsaI CCCAGC 2 cut(s) 276, 1965
GsuI CTGGAG 2 cut(s) 419, 2811
HaeIII GGCC 9 cut(s) 53, 798, 942, 1380, 1644, 1720, 2209, 2917, 3100
HapII CCGG 8 cut(s) 432, 456, 795, 1011, 1166, 1765, 2223, 2506
HhaI GCGC 1 cut(s) 451
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HindIII AAGCTT 4 cut(s) 821, 1339, 1954, 2554
HpaII CCGG 8 cut(s) 432, 456, 795, 1011, 1166, 1765, 2223, 2506
HphI GGTGA 4 cut(s) 881, 1316, 1907, 2030
HpyAV CCTTC 4 cut(s) 353, 656, 1001, 1100
HpyCH4IV ACGT 3 cut(s) 749, 1127, 2431
HpyF10VI GCNNNNNNNGC 7 cut(s) 331, 376, 804, 1962, 2390, 2633, 2741
HpySE526I ACGT 3 cut(s) 749, 1127, 2431
HspAI GCGC 1 cut(s) 449
Kpn2I TCCGGA 3 cut(s) 431, 1165, 2222
KroI GCCGGC 1 cut(s) 2505
KroNI GCCGGC 1 cut(s) 2507
Ksp22I TGATCA 1 cut(s) 162
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 8 cut(s) 763, 1709, 1749, 1761, 1799, 2168, 2225, 2381
Lsp1109I GCAGC 8 cut(s) 179, 690, 778, 1239, 1267, 1753, 2377, 2556
LweI GCATC 4 cut(s) 1614, 2097, 2243, 2695
MabI ACCWGGT 1 cut(s) 1656
MaeI CTAG 7 cut(s) 246, 626, 828, 905, 1140, 2648, 2717
MaeII ACGT 3 cut(s) 749, 1127, 2431
MaeIII GTNAC 6 cut(s) 253, 1979, 2047, 2498, 2763, 2788
MbiI CCGCTC 1 cut(s) 3150
MfeI CAATTG 2 cut(s) 673, 695
MflI RGATCY 2 cut(s) 2797, 3079
MhlI GDGCHC 4 cut(s) 381, 1372, 2230, 2470
MlyI GAGTC 2 cut(s) 261, 289
MmeI TCCRAC 1 cut(s) 715
MroI TCCGGA 3 cut(s) 431, 1165, 2222
MroNI GCCGGC 1 cut(s) 2505
MroXI GAANNNNTTC 2 cut(s) 295, 1000
MslI CAYNNNNRTG 3 cut(s) 1920, 2514, 2577
MspA1I CMGCKG 1 cut(s) 325
MspCI CTTAAG 2 cut(s) 1951, 2618
MspI CCGG 8 cut(s) 432, 456, 795, 1011, 1166, 1765, 2223, 2506
MspR9I CCNGG 4 cut(s) 398, 704, 1658, 3077
MunI CAATTG 2 cut(s) 673, 695
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 4 cut(s) 398, 704, 1658, 3077
MwoI GCNNNNNNNGC 7 cut(s) 331, 376, 804, 1962, 2390, 2633, 2741
NaeI GCCGGC 1 cut(s) 2507
NcoI CCATGG 1 cut(s) 1921
NgoMIV GCCGGC 1 cut(s) 2505
NheI GCTAGC 1 cut(s) 2647
NlaIV GGNNCC 8 cut(s) 641, 893, 1015, 1453, 1763, 1783, 2227, 3081
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 427
NspV TTCGAA 1 cut(s) 994
PagI TCATGA 1 cut(s) 3031
PceI AGGCCT 1 cut(s) 2917
PciSI GCTCTTC 1 cut(s) 610
PctI GAATGC 1 cut(s) 29
PdiI GCCGGC 1 cut(s) 2507
PdmI GAANNNNTTC 2 cut(s) 295, 1000
PfeI GAWTC 9 cut(s) 265, 349, 531, 737, 1312, 1438, 1930, 3008, 3028
PflMI CCANNNNNTGG 2 cut(s) 865, 1753
PleI GAGTC 2 cut(s) 260, 288
PpsI GAGTC 2 cut(s) 260, 288
Ppu21I YACGTR 1 cut(s) 750
PshAI GACNNNNGTC 1 cut(s) 842
Psp124BI GAGCTC 2 cut(s) 381, 2470
Psp1406I AACGTT 1 cut(s) 1127
Psp6I CCWGG 4 cut(s) 396, 702, 1656, 3075
PspFI CCCAGC 2 cut(s) 272, 1961
PspGI CCWGG 4 cut(s) 396, 702, 1656, 3075
PspN4I GGNNCC 8 cut(s) 641, 893, 1015, 1453, 1763, 1783, 2227, 3081
PspPI GGNCC 5 cut(s) 434, 941, 1451, 1782, 3099
PstI CTGCAG 5 cut(s) 376, 1257, 1650, 2861, 2948
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 2797, 3079
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 4 cut(s) 1652, 2006, 2032, 2834
RsaNI GTAC 4 cut(s) 1651, 2005, 2031, 2833
RseI CAYNNNNRTG 3 cut(s) 1920, 2514, 2577
SacI GAGCTC 2 cut(s) 381, 2470
SapI GCTCTTC 1 cut(s) 610
Sau96I GGNCC 5 cut(s) 434, 941, 1451, 1782, 3099
SbfI CCTGCAGG 1 cut(s) 2861
SchI GAGTC 2 cut(s) 261, 289
ScrFI CCNGG 4 cut(s) 398, 704, 1658, 3077
SdaI CCTGCAGG 1 cut(s) 2861
SduI GDGCHC 4 cut(s) 381, 1372, 2230, 2470
SexAI ACCWGGT 1 cut(s) 1656
SfaNI GCATC 4 cut(s) 1614, 2097, 2243, 2695
SfcI CTRYAG 6 cut(s) 372, 1253, 1646, 2857, 2919, 2944
SfuI TTCGAA 1 cut(s) 994
SinI GGWCC 3 cut(s) 434, 1451, 1782
SmiMI CAYNNNNRTG 3 cut(s) 1920, 2514, 2577
SnaBI TACGTA 1 cut(s) 750
Sse8387I CCTGCAGG 1 cut(s) 2861
SseBI AGGCCT 1 cut(s) 2917
SsiI CCGC 3 cut(s) 2210, 2636, 3150
SspI AATATT 1 cut(s) 484
SspMI CTAG 7 cut(s) 246, 626, 828, 905, 1140, 2648, 2717
SstI GAGCTC 2 cut(s) 381, 2470
StuI AGGCCT 1 cut(s) 2917
StyD4I CCNGG 4 cut(s) 396, 702, 1656, 3075
StyI CCWWGG 2 cut(s) 957, 1921
TaiI ACGT 3 cut(s) 752, 1130, 2434
TaqI TCGA 8 cut(s) 313, 467, 979, 994, 1211, 1856, 2080, 2689
TaqII GACCGA 1 cut(s) 65
TatI WGTACW 4 cut(s) 1650, 2004, 2030, 2832
TauI GCSGC 2 cut(s) 2212, 2639
TfiI GAWTC 9 cut(s) 265, 349, 531, 737, 1312, 1438, 1930, 3008, 3028
TscAI CASTG 4 cut(s) 292, 580, 1039, 2289
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 8 cut(s) 192, 678, 766, 1252, 1255, 1741, 2390, 2569
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 6 cut(s) 558, 627, 705, 2589, 2736, 3009
TspGWI ACGGA 3 cut(s) 1639, 1665, 2565
TspRI CASTG 4 cut(s) 292, 580, 1039, 2289
Van91I CCANNNNNTGG 2 cut(s) 865, 1753
Vha464I CTTAAG 2 cut(s) 1951, 2618
VpaK11BI GGWCC 3 cut(s) 434, 1451, 1782
XapI RAATTY 9 cut(s) 463, 1027, 1096, 1294, 1383, 1666, 2260, 2435, 2726
XbaI TCTAGA 2 cut(s) 625, 2716
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 1804
XmiI GTMKAC 2 cut(s) 746, 2303
XmnI GAANNNNTTC 2 cut(s) 295, 1000
XspI CTAG 7 cut(s) 246, 626, 828, 905, 1140, 2648, 2717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.