pycom11g22310
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010431
Physical Location & Seq
Forward (+)
25300578 .. 25303463
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22310.1

Sequence Viewer

Length: 2886 bp
ATGAGTCTCAGGGGAACGATTTCACGCTTTTTGGGTCGATTGGATCAGCTGAAGTTGCTTAATCTTTCTCTGAATCATCTTGAAGGTGGAGTGCCTGCAGAGCTCTCAGACTTGAAGCACCTGGAGGTTCTTGACTTGAGCAATAACATGCTGTCCGGACCAGTTTCAGGTGCGCTTTCCGGTTTGAATTCGATCAGAGTTCTGAATATTTCAAGCAATTCAATCCATGATGATTTGTCTGAGCTTGGAGGAGGATTCTCACATCTTGTTGTGTTCAACATAAGCAATAATTCATTCACTGGTCAGTTCAACCCTCGGATTTGTAGCTCTTCCAATGAAACCCACATTCTAGATATGTCTTGGAACCGTTTGGTGGGAAGTCTTGAAGGCTTAGACAATTGCAGCAGGTCTCTCCAACAATTGCACCTGGACTTCAATTCCTTTGCAGGCTATCTACCTGAATCTTTGTATACGTATTCGGCCTTGGAGCAGCTTTCAGTCTCTGGGAACTCTCTTTCCGGCCAGATAAGCAAGGAACTGAGTAAGCTTTCTAGCCTCAAGACCTTAGTCATTTCTTCAAACCAATTTTTGGGTGAACTTCCAAATGTGTTTGGGAACCTTAGACGACTAGAACAGTTAGTTGCACATTCAAATATGTTGTCTGGCCCATTAGCTTCAACCTTGGCACTCTGTTCAAATCTTCGAGTGCTTGACCTTCGAAACAATTCCCTATCCGGTTCCATTGATCTAAATTTCACTGGACTTCCAAAACTCTGCACTCTTGATCTTGCCACTAATCATTTTTCTGGTTTCCTTCCAAATTCACTTTCTAATTGTCGTGAGTTGAAAACGTTAAGCCTTGCTAGGAATAAATTAAGGGGTTCAATTCCGGAAGACTTTGCCAAACTCACATCCCTATTCTTCCTCTCATTGTCGAATAACAGTTTTGTGAACTTATCTGAGACACTATCTGTGCTACAGCAATGCAAAAATCTCACAACTCTAATTCTTTCAAAAAATTTCCTTGGTGAGGAGATTCCGAAAAATGCAAGTGGGTTTGAAAGCTTAATGGTTTTAGCACTTGGGAATTGTGCTCTCAAAGGCCAAATTCCAGTTTGGTTATTGAGTTGCAGGAAGTTGCAAGTCCTTGATTTATCTTGGAATCGCTTGGATGGCCCCATCCCTTCGTGGATAGGTCAGATGGAAAACTTGTTTTATTTGGACTTTTCAAATAATTCACTCTCGGGAGAAATCCCAAAAAGTATGACGGAGCTGAAAAGCCTCATATCTACGAATTGCACTCATGCAAACCTTAGTGCTTCTGCTGGCATCCCGTTATTTGTAAAACGGAATAGGAGTGCTACTGGCCTGCAGTACAACCAGGTCTCAAATTTTCCTCCGTCAATACTCTTGAGCAATAACAGAATAAATGGAAGAATATGGTCTGAAATCGGACGATTGAAGCAGCTCCATGTTTTGGATTGGAGCAGGAACAACATTAGTGGGACCATCCCAAGCTCCATCTCAGAGATGCAGAACTTGGAAGCAATGGATTTATCATTCAATGATCTCTATGGTTCGATCCCTCCATCACTTAGCAAGCTCACATTCTTGTCAAAGTTTAGCGTGGCAAATAATCACCTCCATGGAGTGATTCCCAATGGCGAACAGTTCTTAAGCTTTCCCAGCTCAAGTTTTGACGGTAACTCGGGGCTTTGTGGGGCAATGTACACTCCATGTGGTGATATCAGCAGTACAAGTCTCAAGCCTGTAACGCCATCCAGTTCAAACAGCAGATTTGGTCGAAACAGCATCCTTTGTGTAGTAATCAGCATAGGAGTTGGGGTTGCATTGCTTCTTGCAGTTGGTTTGCTTAAAATGTCAAGGAGGGGAGCAAAGGATCAAATTGATGATTTTGATGAGGACAGCAGGCCGCACAGGATATCCGGAGCCCTTGCATCGTCAAAACTGGTGCTTTTTCAAAATTCAGACTGTAAGGATTATACAGTGTCAGACTTGTTAAAGTCTACAAACAATTTCAACCAAGCCAACATAATTGGTTGTGGCGGTTATGGACTGGTTTACAAGGCCAACCTACCGAATGGAGCAAAAGCTGCAATCAAGAGACTTTCGGGGGATTGTGGACAGATGGAACGTGAATTTCAAGCTGAAGTGGAAGCCCTCTCAAGAGCTCAGCACAAAAACCTTGTCTCCCTTCAAGGTTACTGCCGGCATGGTAATGACAGACTGTTAATCTACTCCTACATGGAGAACGGAAGCTTGGATTATTGGCTGCATGAAAGTGTTGATGGTGTTTCGCTTCTAAAATGGGATGCAAGACTTAAGATAGCTCAAGGTGCGGCTCGTGGGTTAGCCTACTTGCACAAGGGTTGTCAGCCAAATATAGTGCATCGAGATATAAAAACAAGCAACATTCTTCTAGATGAAAAATTTGAAGCTCACTTGGCTGATTTTGGTCTTTCAAGGTTACTTCGTCCTTACGATACCCATGTTACAACAGATCTGGTTGGTACTTTGGGTTATATTCCTCCAGAGTACTGTCAGACATTAACAGCAACCTGCAGGGGCGATGTTTACAGTTTTGGTGTTGTTCTTCTTGAGCTTCTTACTGGTAGGAGGCCTGTAGAAGTATGCAGAGGAAAAAACTGCAGAGATTTGGTATCATGGATGTTTCAGATGAGATTTGAGAAGAGAGATGAAGAGATTATAGATTCGTCAATTTGGAATAAGAATCATGAGAAGCAACTTTTAGATGTTCTTGGTGTTGCCTGTAAATGCCTGGATCCCAATCCGAGGCAGAGGCCCTTTATTGAAGAAGTTGTGTCATGCCTTGACGGTATAGGATTTGAGAGCGGGAAACAATGA

Protein Analysis

962

Amino Acids

105.63

Weight (kDa)

7.25

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 16 - 75 3.4e-07 Leucine rich repeat
LRR_8 PF13855 136 - 196 9.9e-06 Leucine rich repeat
LRR_14 PF23598 228 - 335 4.7e-06 Leucine-rich repeat region
LRR_8 PF13855 232 - 292 1e-08 Leucine rich repeat
LRR_8 PF13855 275 - 315 4.8e-06 Leucine rich repeat
LRR_8 PF13855 331 - 390 7.2e-06 Leucine rich repeat
Pkinase PF00069 681 - 948 1.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 683 - 951 2.7e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 396, 2588
AccB7I CCANNNNNTGG 2 cut(s) 589, 1477
AccBSI CCGCTC 1 cut(s) 2874
AccI GTMKAC 2 cut(s) 470, 2027
AccIII TCCGGA 3 cut(s) 155, 889, 1946
AciI CCGC 4 cut(s) 1934, 2067, 2360, 2874
AclI AACGTT 1 cut(s) 851
AclWI GGATC 5 cut(s) 51, 1576, 1908, 2798, 2811
AcoI YGGCCR 1 cut(s) 520
AcsI RAATTY 9 cut(s) 187, 751, 820, 1018, 1107, 1390, 1984, 2159, 2450
AcuI CTGAAG 2 cut(s) 71, 2190
AfaI GTAC 5 cut(s) 1376, 1730, 1756, 2533, 2558
AfiI CCNNNNNNNGG 6 cut(s) 167, 373, 589, 1030, 1477, 2814
AflII CTTAAG 2 cut(s) 1675, 2342
AjnI CCWGG 4 cut(s) 120, 426, 1380, 2799
AjuI GAANNNNNNNTTGG 4 cut(s) 811, 843, 2264, 2296
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
Alw21I GWGCWC 3 cut(s) 105, 1096, 2194
Alw26I GTCTC 8 cut(s) 11, 414, 505, 956, 1390, 1766, 2119, 2215
AlwI GGATC 5 cut(s) 51, 1576, 1908, 2798, 2811
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 2 cut(s) 1243, 1708
Aor13HI TCCGGA 3 cut(s) 155, 889, 1946
ApeKI GCWGC 5 cut(s) 402, 490, 1465, 2114, 2293
ApoI RAATTY 9 cut(s) 187, 751, 820, 1018, 1107, 1390, 1984, 2159, 2450
ArsI GACNNNNNNTTYG 4 cut(s) 644, 676, 2193, 2225
Asp700I GAANNNNTTC 2 cut(s) 19, 724
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 5 cut(s) 158, 665, 1175, 1506, 2823
AsuHPI GGTGA 4 cut(s) 605, 1040, 1631, 1754
AsuII TTCGAA 1 cut(s) 718
AvaI CYCGRG 2 cut(s) 1243, 1708
AvaII GGWCC 2 cut(s) 158, 1506
BaeI ACNNNNGTAYC 2 cut(s) 2496, 2529
BamHI GGATCC 1 cut(s) 2803
BanII GRGCYC 3 cut(s) 105, 1954, 2194
BauI CACGAG 1 cut(s) 2364
BbsI GAAGAC 1 cut(s) 900
Bbv12I GWGCWC 3 cut(s) 105, 1096, 2194
BbvI GCAGC 5 cut(s) 414, 502, 1477, 2101, 2280
BccI CCATC 9 cut(s) 1166, 1187, 1195, 1517, 1529, 1597, 1786, 2143, 2303
BciT130I CCWGG 4 cut(s) 122, 428, 1382, 2801
BcoDI GTCTC 8 cut(s) 11, 414, 505, 956, 1390, 1766, 2119, 2215
BfaI CTAG 5 cut(s) 350, 552, 629, 864, 2441
BfmI CTRYAG 6 cut(s) 96, 977, 1370, 2581, 2643, 2668
BfrI CTTAAG 2 cut(s) 1675, 2342
BfuAI ACCTGC 2 cut(s) 396, 2588
BglII AGATCT 1 cut(s) 2521
BisI GCNGC 7 cut(s) 403, 491, 1466, 1934, 2115, 2294, 2361
BlpI GCTNAGC 1 cut(s) 2193
BlsI GCNGC 7 cut(s) 404, 492, 1467, 1935, 2116, 2295, 2362
BmcAI AGTACT 1 cut(s) 2558
Bme1390I CCNGG 4 cut(s) 122, 428, 1382, 2801
Bme18I GGWCC 2 cut(s) 158, 1506
BmeT110I CYCGRG 2 cut(s) 1243, 1708
BmgT120I GGNCC 5 cut(s) 158, 665, 1175, 1506, 2823
BmiI GGNNCC 7 cut(s) 365, 617, 739, 1177, 1507, 1951, 2805
BmrFI CCNGG 4 cut(s) 122, 428, 1382, 2801
BmsI GCATC 6 cut(s) 1338, 1521, 1821, 1967, 2323, 2419
BoxI GACNNNNGTC 1 cut(s) 566
BpiI GAAGAC 1 cut(s) 900
BpmI CTGGAG 2 cut(s) 143, 2535
Bpu1102I GCTNAGC 1 cut(s) 2193
Bpu14I TTCGAA 1 cut(s) 718
BpuEI CTTGAG 8 cut(s) 157, 542, 1432, 1675, 1748, 2170, 2337, 2639
BsaAI YACGTR 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 2808
BsaI GGTCTC 2 cut(s) 414, 1390
BsaJI CCNNGG 6 cut(s) 314, 483, 681, 1024, 1645, 2813
BsaWI WCCGGW 5 cut(s) 155, 179, 734, 889, 1946
BsaXI ACNNNNNCTCC 4 cut(s) 1830, 1860, 2195, 2225
Bsc4I CCNNNNNNNGG 6 cut(s) 167, 373, 589, 1030, 1477, 2814
Bse118I RCCGGY 1 cut(s) 2229
Bse1I ACTGG 9 cut(s) 161, 304, 763, 1112, 1369, 1782, 1974, 2082, 2635
Bse3DI GCAATG 4 cut(s) 989, 1554, 1731, 1850
Bse8I GATNNNNATC 1 cut(s) 2808
BseAI TCCGGA 3 cut(s) 155, 889, 1946
BseBI CCWGG 4 cut(s) 122, 428, 1382, 2801
BseDI CCNNGG 6 cut(s) 314, 483, 681, 1024, 1645, 2813
BseGI GGATG 9 cut(s) 911, 1177, 1179, 1329, 1509, 1778, 1812, 2338, 2694
BseJI GATNNNNATC 1 cut(s) 2808
BseLI CCNNNNNNNGG 6 cut(s) 167, 373, 589, 1030, 1477, 2814
BseMI GCAATG 4 cut(s) 989, 1554, 1731, 1850
BseMII CTCAG 7 cut(s) 22, 120, 231, 530, 951, 1539, 2207
BseNI ACTGG 9 cut(s) 161, 304, 763, 1112, 1369, 1782, 1974, 2082, 2635
BseRI GAGGAG 2 cut(s) 264, 1046
BseXI GCAGC 5 cut(s) 414, 502, 1477, 2101, 2280
BseYI CCCAGC 1 cut(s) 1685
BsgI GTGCAG 1 cut(s) 760
BsiHKAI GWGCWC 3 cut(s) 105, 1096, 2194
BsiHKCI CYCGRG 2 cut(s) 1243, 1708
BsiSI CCGG 7 cut(s) 156, 180, 519, 735, 890, 1947, 2230
BslFI GGGAC 1 cut(s) 1519
BslI CCNNNNNNNGG 6 cut(s) 167, 373, 589, 1030, 1477, 2814
BsmAI GTCTC 8 cut(s) 11, 414, 505, 956, 1390, 1766, 2119, 2215
BsmFI GGGAC 1 cut(s) 1519
Bso31I GGTCTC 2 cut(s) 414, 1390
BsoBI CYCGRG 2 cut(s) 1243, 1708
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 4 cut(s) 105, 1096, 1954, 2194
Bsp13I TCCGGA 3 cut(s) 155, 889, 1946
Bsp1407I TGTACA 1 cut(s) 1728
Bsp143I GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
Bsp1720I GCTNAGC 1 cut(s) 2193
Bsp19I CCATGG 1 cut(s) 1645
BspACI CCGC 4 cut(s) 1934, 2067, 2360, 2874
BspCNI CTCAG 7 cut(s) 21, 119, 232, 531, 952, 1538, 2206
BspEI TCCGGA 3 cut(s) 155, 889, 1946
BspHI TCATGA 1 cut(s) 2755
BspLI GGNNCC 7 cut(s) 365, 617, 739, 1177, 1507, 1951, 2805
BspMAI CTGCAG 4 cut(s) 100, 1374, 2585, 2672
BspMI ACCTGC 2 cut(s) 396, 2588
BspPI GGATC 5 cut(s) 51, 1576, 1908, 2798, 2811
BspQI GCTCTTC 1 cut(s) 334
BspT104I TTCGAA 1 cut(s) 718
BspTI CTTAAG 2 cut(s) 1675, 2342
BspTNI GGTCTC 2 cut(s) 414, 1390
BsrBI CCGCTC 1 cut(s) 2874
BsrDI GCAATG 4 cut(s) 989, 1554, 1731, 1850
BsrFI RCCGGY 1 cut(s) 2229
BsrGI TGTACA 1 cut(s) 1728
BsrI ACTGG 9 cut(s) 161, 304, 763, 1112, 1369, 1782, 1974, 2082, 2635
BssAI RCCGGY 1 cut(s) 2229
BssECI CCNNGG 6 cut(s) 314, 483, 681, 1024, 1645, 2813
BssMI GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
BssNAI GTATAC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 2364
BssT1I CCWWGG 4 cut(s) 483, 681, 1024, 1645
Bst1107I GTATAC 1 cut(s) 471
Bst2BI CACGAG 1 cut(s) 2364
Bst2UI CCWGG 4 cut(s) 122, 428, 1382, 2801
Bst6I CTCTTC 3 cut(s) 334, 2705, 2715
BstAFI CTTAAG 2 cut(s) 1675, 2342
BstAPI GCANNNNNTGC 1 cut(s) 2114
BstAUI TGTACA 1 cut(s) 1728
BstBAI YACGTR 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 7 cut(s) 96, 448, 1327, 1370, 1601, 1931, 2231
BstDSI CCRYGG 1 cut(s) 1645
BstENI CCTNNNNNAGG 1 cut(s) 1028
BstF5I GGATG 9 cut(s) 911, 1177, 1179, 1329, 1509, 1778, 1812, 2338, 2694
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 9 cut(s) 46, 195, 748, 787, 1570, 1584, 1903, 2524, 2806
BstMAI GTCTC 8 cut(s) 11, 414, 505, 956, 1390, 1766, 2119, 2215
BstMBI GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
BstMWI GCNNNNNNNGC 9 cut(s) 55, 100, 528, 1173, 1686, 1774, 2114, 2357, 2465
BstNI CCWGG 4 cut(s) 122, 428, 1382, 2801
BstNSI RCATGY 1 cut(s) 151
BstPAI GACNNNNGTC 1 cut(s) 566
BstSCI CCNGG 4 cut(s) 120, 426, 1380, 2799
BstSFI CTRYAG 6 cut(s) 96, 977, 1370, 2581, 2643, 2668
BstSNI TACGTA 1 cut(s) 474
BstV1I GCAGC 5 cut(s) 414, 502, 1477, 2101, 2280
BstV2I GAAGAC 1 cut(s) 900
BstX2I RGATCY 2 cut(s) 2521, 2803
BstYI RGATCY 2 cut(s) 2521, 2803
BstZ17I GTATAC 1 cut(s) 471
BtgI CCRYGG 1 cut(s) 1645
BtgZI GCGATG 1 cut(s) 2604
BtsCI GGATG 9 cut(s) 911, 1177, 1179, 1329, 1509, 1778, 1812, 2338, 2694
BtsIMutI CAGTG 3 cut(s) 297, 756, 2013
BveI ACCTGC 2 cut(s) 396, 2588
Cac8I GCNNGC 7 cut(s) 96, 448, 1327, 1370, 1601, 1931, 2231
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 2755
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 2229
Cfr13I GGNCC 5 cut(s) 158, 665, 1175, 1506, 2823
CsiI ACCWGGT 1 cut(s) 1380
Csp6I GTAC 5 cut(s) 1375, 1729, 1755, 2532, 2557
CspCI CAANNNNNGTGG 2 cut(s) 1483, 1518
CviQI GTAC 5 cut(s) 1375, 1729, 1755, 2532, 2557
DpnI GATC 9 cut(s) 45, 194, 747, 786, 1569, 1583, 1902, 2523, 2805
DpnII GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
EaeI YGGCCR 1 cut(s) 520
Eam1104I CTCTTC 3 cut(s) 334, 2705, 2715
EarI CTCTTC 3 cut(s) 334, 2705, 2715
Ecl136II GAGCTC 2 cut(s) 103, 2192
Eco105I TACGTA 1 cut(s) 474
Eco130I CCWWGG 4 cut(s) 483, 681, 1024, 1645
Eco147I AGGCCT 1 cut(s) 2641
Eco24I GRGCYC 3 cut(s) 105, 1954, 2194
Eco31I GGTCTC 2 cut(s) 414, 1390
Eco32I GATATC 2 cut(s) 1747, 1944
Eco47I GGWCC 2 cut(s) 158, 1506
Eco53kI GAGCTC 2 cut(s) 103, 2192
Eco57I CTGAAG 2 cut(s) 71, 2190
Eco88I CYCGRG 2 cut(s) 1243, 1708
EcoICRI GAGCTC 2 cut(s) 103, 2192
EcoNI CCTNNNNNAGG 1 cut(s) 1028
EcoO109I RGGNCCY 1 cut(s) 2823
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 4 cut(s) 120, 426, 1380, 2799
EcoRV GATATC 2 cut(s) 1747, 1944
EcoT14I CCWWGG 4 cut(s) 483, 681, 1024, 1645
EcoT38I GRGCYC 3 cut(s) 105, 1954, 2194
ErhI CCWWGG 4 cut(s) 483, 681, 1024, 1645
FaqI GGGAC 1 cut(s) 1519
FauI CCCGC 1 cut(s) 2867
FblI GTMKAC 2 cut(s) 470, 2027
Fnu4HI GCNGC 7 cut(s) 403, 491, 1466, 1934, 2115, 2294, 2361
FokI GGATG 9 cut(s) 898, 1166, 1184, 1316, 1496, 1765, 1799, 2345, 2701
FriOI GRGCYC 3 cut(s) 105, 1954, 2194
Fsp4HI GCNGC 7 cut(s) 403, 491, 1466, 1934, 2115, 2294, 2361
FspBI CTAG 5 cut(s) 350, 552, 629, 864, 2441
GlaI GCGC 1 cut(s) 174
GluI GCNGC 7 cut(s) 403, 491, 1466, 1934, 2115, 2294, 2361
GsaI CCCAGC 1 cut(s) 1689
GsuI CTGGAG 2 cut(s) 143, 2535
HapII CCGG 7 cut(s) 156, 180, 519, 735, 890, 1947, 2230
HhaI GCGC 1 cut(s) 175
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 4 cut(s) 545, 1063, 1678, 2278
HinfI GANTC 9 cut(s) 4, 73, 255, 461, 1036, 1162, 1654, 2732, 2752
HpaII CCGG 7 cut(s) 156, 180, 519, 735, 890, 1947, 2230
HphI GGTGA 4 cut(s) 605, 1040, 1631, 1754
Hpy166II GTNNAC 8 cut(s) 471, 596, 952, 1731, 2028, 2083, 2144, 2596
Hpy8I GTNNAC 8 cut(s) 471, 596, 952, 1731, 2028, 2083, 2144, 2596
HpyAV CCTTC 6 cut(s) 77, 380, 725, 824, 1194, 2225
HpyCH4IV ACGT 3 cut(s) 473, 851, 2155
HpyF10VI GCNNNNNNNGC 9 cut(s) 55, 100, 528, 1173, 1686, 1774, 2114, 2357, 2465
HpySE526I ACGT 3 cut(s) 473, 851, 2155
HspAI GCGC 1 cut(s) 173
Kpn2I TCCGGA 3 cut(s) 155, 889, 1946
KroI GCCGGC 1 cut(s) 2229
KroNI GCCGGC 1 cut(s) 2231
Kzo9I GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 8 cut(s) 487, 1270, 1473, 1485, 1523, 1892, 1949, 2105
Lsp1109I GCAGC 5 cut(s) 414, 502, 1477, 2101, 2280
LweI GCATC 6 cut(s) 1338, 1521, 1821, 1967, 2323, 2419
MabI ACCWGGT 1 cut(s) 1380
MaeI CTAG 5 cut(s) 350, 552, 629, 864, 2441
MaeII ACGT 3 cut(s) 473, 851, 2155
MaeIII GTNAC 5 cut(s) 1703, 1771, 2222, 2487, 2512
MalI GATC 9 cut(s) 45, 194, 747, 786, 1569, 1583, 1902, 2523, 2805
MbiI CCGCTC 1 cut(s) 2874
MboI GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
MfeI CAATTG 2 cut(s) 397, 419
MflI RGATCY 2 cut(s) 2521, 2803
MhlI GDGCHC 4 cut(s) 105, 1096, 1954, 2194
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 439
MroI TCCGGA 3 cut(s) 155, 889, 1946
MroNI GCCGGC 1 cut(s) 2229
MroXI GAANNNNTTC 2 cut(s) 19, 724
MslI CAYNNNNRTG 3 cut(s) 1644, 2238, 2301
MspA1I CMGCKG 1 cut(s) 49
MspCI CTTAAG 2 cut(s) 1675, 2342
MspI CCGG 7 cut(s) 156, 180, 519, 735, 890, 1947, 2230
MspR9I CCNGG 4 cut(s) 122, 428, 1382, 2801
MunI CAATTG 2 cut(s) 397, 419
MvaI CCWGG 4 cut(s) 122, 428, 1382, 2801
MwoI GCNNNNNNNGC 9 cut(s) 55, 100, 528, 1173, 1686, 1774, 2114, 2357, 2465
NaeI GCCGGC 1 cut(s) 2231
NcoI CCATGG 1 cut(s) 1645
NdeII GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
NgoMIV GCCGGC 1 cut(s) 2229
NlaIV GGNNCC 7 cut(s) 365, 617, 739, 1177, 1507, 1951, 2805
NspI RCATGY 1 cut(s) 151
NspV TTCGAA 1 cut(s) 718
PagI TCATGA 1 cut(s) 2755
PceI AGGCCT 1 cut(s) 2641
PciSI GCTCTTC 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 2231
PdmI GAANNNNTTC 2 cut(s) 19, 724
PfeI GAWTC 8 cut(s) 73, 255, 461, 1036, 1162, 1654, 2732, 2752
PflMI CCANNNNNTGG 2 cut(s) 589, 1477
PkrI GCNGC 7 cut(s) 404, 492, 1467, 1935, 2116, 2295, 2362
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 474
PshAI GACNNNNGTC 1 cut(s) 566
Psp124BI GAGCTC 2 cut(s) 105, 2194
Psp1406I AACGTT 1 cut(s) 851
Psp6I CCWGG 4 cut(s) 120, 426, 1380, 2799
PspFI CCCAGC 1 cut(s) 1685
PspGI CCWGG 4 cut(s) 120, 426, 1380, 2799
PspN4I GGNNCC 7 cut(s) 365, 617, 739, 1177, 1507, 1951, 2805
PspPI GGNCC 5 cut(s) 158, 665, 1175, 1506, 2823
PstI CTGCAG 4 cut(s) 100, 1374, 2585, 2672
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 2 cut(s) 2521, 2803
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 5 cut(s) 1376, 1730, 1756, 2533, 2558
RsaNI GTAC 5 cut(s) 1375, 1729, 1755, 2532, 2557
RseI CAYNNNNRTG 3 cut(s) 1644, 2238, 2301
SacI GAGCTC 2 cut(s) 105, 2194
SapI GCTCTTC 1 cut(s) 334
SatI GCNGC 7 cut(s) 403, 491, 1466, 1934, 2115, 2294, 2361
Sau3AI GATC 9 cut(s) 43, 192, 745, 784, 1567, 1581, 1900, 2521, 2803
Sau96I GGNCC 5 cut(s) 158, 665, 1175, 1506, 2823
SbfI CCTGCAGG 1 cut(s) 2585
ScaI AGTACT 1 cut(s) 2558
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 4 cut(s) 122, 428, 1382, 2801
SdaI CCTGCAGG 1 cut(s) 2585
SduI GDGCHC 4 cut(s) 105, 1096, 1954, 2194
SexAI ACCWGGT 1 cut(s) 1380
SfaNI GCATC 6 cut(s) 1338, 1521, 1821, 1967, 2323, 2419
SfcI CTRYAG 6 cut(s) 96, 977, 1370, 2581, 2643, 2668
SfuI TTCGAA 1 cut(s) 718
SinI GGWCC 2 cut(s) 158, 1506
SmiMI CAYNNNNRTG 3 cut(s) 1644, 2238, 2301
SnaBI TACGTA 1 cut(s) 474
Sse8387I CCTGCAGG 1 cut(s) 2585
SseBI AGGCCT 1 cut(s) 2641
SsiI CCGC 4 cut(s) 1934, 2067, 2360, 2874
SspI AATATT 1 cut(s) 208
SspMI CTAG 5 cut(s) 350, 552, 629, 864, 2441
SstI GAGCTC 2 cut(s) 105, 2194
StuI AGGCCT 1 cut(s) 2641
StyD4I CCNGG 4 cut(s) 120, 426, 1380, 2799
StyI CCWWGG 4 cut(s) 483, 681, 1024, 1645
TaiI ACGT 3 cut(s) 476, 854, 2158
TaqI TCGA 8 cut(s) 37, 191, 703, 718, 935, 1580, 1804, 2413
TatI WGTACW 4 cut(s) 1374, 1728, 1754, 2556
TauI GCSGC 2 cut(s) 1936, 2363
TfiI GAWTC 8 cut(s) 73, 255, 461, 1036, 1162, 1654, 2732, 2752
TscAI CASTG 3 cut(s) 304, 763, 2013
TseI GCWGC 5 cut(s) 402, 490, 1465, 2114, 2293
TspDTI ATGAA 5 cut(s) 282, 351, 2313, 2460, 2733
TspGWI ACGGA 4 cut(s) 1283, 1363, 1389, 2289
TspRI CASTG 3 cut(s) 304, 763, 2013
Van91I CCANNNNNTGG 2 cut(s) 589, 1477
Vha464I CTTAAG 2 cut(s) 1675, 2342
VpaK11BI GGWCC 2 cut(s) 158, 1506
XagI CCTNNNNNAGG 1 cut(s) 1028
XapI RAATTY 9 cut(s) 187, 751, 820, 1018, 1107, 1390, 1984, 2159, 2450
XbaI TCTAGA 2 cut(s) 349, 2440
XceI RCATGY 1 cut(s) 151
XmiI GTMKAC 2 cut(s) 470, 2027
XmnI GAANNNNTTC 2 cut(s) 19, 724
XspI CTAG 5 cut(s) 350, 552, 629, 864, 2441
ZrmI AGTACT 1 cut(s) 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.