Rw5G020830
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010431
Physical Location & Seq
Reverse (-)
27605701 .. 27609726
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020830.1

Sequence Viewer

Length: 3156 bp
ATGGTGGTGCTGGGGATCATTCCAAGGACATTCATCAAATGGGTTTTCTTGGCTTGCTTTCTTGGTTCTTCTTTGGGTCTTGATGCCCCAATCCAATCCTGTGATCCAAGTGATCTTCTTGCACTCAAGGGGTTTGCAGGAAACCTTACAAGTGGGTCTATTATCACAGCATGGTCCAAGAACTCAAATTGCTGCCAATGGGATGGTGTGGTTTGTGAGAGTGTGAATAATGGTACAGCTGCAAGCAGAGTCACCAAGTTGTTTCTTCCCAACATGAGTCTCAATGGAACAATTTCGCGCTCTTTGGGTGGATTGGATCAGCTGCAATTGCTTAATCTTTCTCTGAATCATCTTGGAGGTGGATTGCCAGCAGAGTTCTCAAACTTGAAGCGGCTGCAAGTTCTTGATTTGAGCTACAACAGGCTTTCAGGACCAGTTTCAGGTGTGCTTGCTAGTTTGAAGTCCATTAAAGTACTGAACATTTCAAGCAATTCAATCAGTGGTGAATTATCTGAGCTCGGGGGTTTCTCAAATCTTGTGGTGTTCAACATAAGCAATAATTCATTCACCGGTCAGTTCAATCCTCAAATTTGTAGCTCTTCCAATGCAATTCATATTCTTGATATGTCTTTGAACCATTTCACCGGCAGTCTTGAAGGCTTAGGCAATTGTAGCACATCTCTCCAGCAATTGCACCTGGACTTCAATTCATTTGCAGGCCAGCTACCTGAATCTTTGTATTCCTTTTTAGCCTTGGAGCAGCTTTCAGTCTCTGGGAACTCTCTTTCTGGCCAGATAAGCAACAAACTGAGTAAGTTCTCTAGACTCAAGACATTACTCATTTGTGGAAACCAATTTGTTGGTGAACTTCCAAATGTTTTTGGGAACCTTAGTCGACTAGAACAGTTAGTTGCACATTCAAATTTTTTATCTGGGCCATTGCCTTCAACTTTGGCACTCTGCTCAAATCTTCGAGTGCTTGATCTTCGAAACAATTCTCTATCAGGTTCTATTGATCTTAATTTCACTGGCCTTTCCAAACTCTGCACCCTTGACCTTGCTACTAATCGTTTTTCGGGCTTCCTTCCAAATTCACTGTCATATTGTCGTGAGTTGAAAACCGTAAGCCTTGCCAAGAATGAGTTAAGGGGTTCAGTCCCTGAAGACTTTGCCAAACTCACGTCCCTATCCTTTCTTTCATTGTCAAATAACAGTTTTGTAAACTTGTCTGGGGCACTATCTGTGCTACAGAAGTGCAAAAACCTCACCATTCTCATTCTTACCAAAAATTTCCTTGGTGAGGAGATTCCCAAAAATGCAAGTGGCTTTGAAAGGTTAATGGTTTTAGCACTTGGAAATTCTGCTCTTAAAGGCCAAATTCCAGCTTGGTTATTGAGTTGCAAGAAGTTGGAAGTCCTTGATTTGTCTTGGAATCACTTGGATGGCAATATCCCTCCATGGATTGGTCAGATGGAGAACTTGTTTTATGTGGACTTTTCAAATAATTCTCTCTCTGGAGAAATCCCAAAAGGTCTAACAGAGCTGAAAAGCCTCATTTCTACAAATAGCAGTCGATCATATCTTACTGCCTCTGCTGGTATCCCGCTATTTGTGAAGCGAAATAAGAGTGCTAATGGACTGCAATACAACCAGGCCTCGAGTTTTCCACCTTCAATATACTTGAGCAATAATAGAATTAATGGAACAATCTGGCCTGAAGTCGGACGACTAAAACAGCTCCATGTTTTGGATTGGAGCAGGAACAACATTACTGGGATCATTCCTAGCTCAATCTCAGAGATGGAGAACTTGGAAACGTTGGATTTATCATGGAATAATCTGTATGGTTCCATCCCTTCATCACTTAGCAAGCTCACATTCTTGTCAAAGTTTAGTGTGGCAAATAATCACTTGCATGGAGTCATTCCCAATGAAGGACAGTTCTTGAGCTTTCCCAACTCAAGCTTTGAAGGCAACCCAGGACTTTGTGGGGAAATATTTTCTCCATGTAATGCCAAGAATGCAGGTCACAAGCCTGTAATGCCATCAAGTTCAAGCAGTAGATTTGGTCGGAACAGTATCCTTTTAGTAACACTCAGCATAGGAGTTGGGATTGCACTGCTTCTTGCAATTGGTCTACTTAGAATGTCAAGGAGGGAAGCTGAAAATCAAATTGATGATTTTGATGAGGAACACAGCAGGCCTCACAGGTTATCAGAAGCACTTGCATCTTCAAAATTGGTGCTTTTTCAGAATGCAAGTTGTAGGGATTTCACAGTTGCAGAGTTGTTAAAATCTACAAACAATTTTAACCAGGCCAACATAATTGGTTGTGGTGGTTATGGGCTGGTTTACAAAGCCAACCTACCTAATGGCACAAAAGCTGCAATCAAGAGACTTTCTGGGGAATGTGGACAGATGGAGCGTGAATTTCAAGCTGAAGTGGAAGCCCTCTCAAGAGCTCAGCACAAGAACCTTGTCTCTCTTCAGGGCTACTGCAAGCATGGTAATGACAGGTTGTTAATTTATTCTTACATGGAGAATGGAAGCTTGGATTATTGGCTGCATGAAAGTGTTGATGGGATTTCACCTCTCAAATGGGATGTAAGACTCAAGATAGCTCAAGGTGCTGCTCACGGATTGGCTTACTTGCATAAGGGCTGTCAGCCTAATATTGTCCATCGAGATATAAAAACGAGCAACATTCTTCTAGATGAGAAATTTGAAGCTCATCTGGCTGATTTTGGTCTCTCAAGGCTACTTCGTCCTTATGACACCCATGTCACAACAGATTTAGTTGGAACTTTGGGCTATATTCCTCCGGAGTATGGTCAGACGTTAACTGCAACCTGCAGGGGTGATGTTTACAGTTTTGGTGTTGTTCTTCTTGAACTTCTTACGGGCAGAAGACCTGTTGAAGTGTGCAAAGGGAAAAATTGCAGAGATTTGGTATCATGGATGTTTCAGATGAGACGTGAGAAGAGAGAGGAGGAAATTATAGATTCATCAATCTGGAATAAGGATCATGAGAAGCAGCTTCTGGAGGTACTTGATGTTGCCTGTAAATGCCTAGATCCCAATCCCAGGCAGAGACCCTTTATCGAAGAAGTTGTATCATGGCTTAATGGTATTGGATTCCAGAGTGAGAAACAATGA

Protein Analysis

1051

Amino Acids

115.14

Weight (kDa)

7.9

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 72 4e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 98 - 256 7.9e-07 Leucine-rich repeat region
LRR_8 PF13855 107 - 166 2.8e-06 Leucine rich repeat
LRR_8 PF13855 322 - 382 8e-07 Leucine rich repeat
LRR_8 PF13855 348 - 405 1.3e-06 Leucine rich repeat
LRR_8 PF13855 421 - 480 3.1e-06 Leucine rich repeat
LRR_8 PF13855 558 - 614 2.3e-07 Leucine rich repeat
Pkinase PF00069 771 - 1038 4.2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 773 - 1039 7.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2780
Acc36I ACCTGC 2 cut(s) 2015, 2858
AccB7I CCANNNNNTGG 2 cut(s) 1463, 1747
AccI GTMKAC 2 cut(s) 895, 2137
AccII CGCG 1 cut(s) 298
AccIII TCCGGA 1 cut(s) 2821
AciI CCGC 2 cut(s) 391, 1604
AclI AACGTT 1 cut(s) 1817
AclWI GGATC 6 cut(s) 23, 98, 324, 1784, 3030, 3068
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
AcuI CTGAAG 4 cut(s) 1182, 1737, 2460, 2471
AfaI GTAC 3 cut(s) 235, 474, 3048
AfiI CCNNNNNNNGG 8 cut(s) 440, 1300, 1463, 1721, 1747, 1934, 2828, 3084
AgeI ACCGGT 1 cut(s) 569
AjiI CACGTC 2 cut(s) 1182, 2975
AjnI CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
AjuI GAANNNNNNNTTGG 2 cut(s) 2534, 2566
Alw21I GWGCWC 2 cut(s) 519, 2464
Alw26I GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
AlwI GGATC 6 cut(s) 23, 98, 324, 1784, 3030, 3068
AlwNI CAGNNNCTG 3 cut(s) 773, 1160, 3040
Ama87I CYCGRG 2 cut(s) 518, 1657
Aor13HI TCCGGA 1 cut(s) 2821
AoxI GGCC 9 cut(s) 718, 790, 935, 1030, 1372, 1653, 1712, 2201, 2316
ApeKI GCWGC 9 cut(s) 192, 239, 322, 394, 760, 2384, 2563, 2630, 3034
ApoI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
ArsI GACNNNNNNTTYG 2 cut(s) 1082, 1114
AseI ATTAAT 1 cut(s) 1698
AsiGI ACCGGT 1 cut(s) 569
Asp700I GAANNNNTTC 3 cut(s) 292, 638, 994
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 174, 431, 935
AsuHPI GGTGA 9 cut(s) 244, 515, 559, 634, 875, 1258, 1310, 2580, 2870
AsuII TTCGAA 1 cut(s) 988
AvaI CYCGRG 2 cut(s) 518, 1657
AvaII GGWCC 2 cut(s) 174, 431
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 792
BanII GRGCYC 2 cut(s) 519, 2464
BbsI GAAGAC 2 cut(s) 1170, 2914
Bbv12I GWGCWC 2 cut(s) 519, 2464
BbvI GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BccI CCATC 9 cut(s) 197, 1436, 1465, 1795, 1859, 2053, 2413, 2573, 2688
BciT130I CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BciVI GTATCC 2 cut(s) 1610, 2090
BcoDI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BfaI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
BfmI CTRYAG 2 cut(s) 1247, 2851
BfuAI ACCTGC 2 cut(s) 2015, 2858
BfuI GTATCC 2 cut(s) 1610, 2090
BlpI GCTNAGC 1 cut(s) 2463
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
Bme18I GGWCC 2 cut(s) 174, 431
BmeT110I CYCGRG 2 cut(s) 518, 1657
BmgBI CACGTC 2 cut(s) 1182, 2975
BmgT120I GGNCC 3 cut(s) 174, 431, 935
BmiI GGNNCC 2 cut(s) 887, 1849
BmrFI CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BmrI ACTGGG 1 cut(s) 1782
BmsI GCATC 2 cut(s) 73, 2237
BmuI ACTGGG 1 cut(s) 1782
BpiI GAAGAC 2 cut(s) 1170, 2914
BpmI CTGGAG 3 cut(s) 668, 1536, 3062
Bpu10I CCTNAGC 1 cut(s) 661
Bpu1102I GCTNAGC 1 cut(s) 2463
Bpu14I TTCGAA 1 cut(s) 988
BpuEI CTTGAG 9 cut(s) 110, 812, 1702, 1945, 1966, 2440, 2597, 2607, 2737
BsaBI GATNNNNATC 1 cut(s) 3078
BsaI GGTCTC 2 cut(s) 2753, 3085
BsaJI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BsaWI WCCGGW 2 cut(s) 569, 2821
BsaXI ACNNNNNCTCC 2 cut(s) 1466, 1496
Bsc4I CCNNNNNNNGG 8 cut(s) 440, 1300, 1463, 1721, 1747, 1934, 2828, 3084
Bse118I RCCGGY 2 cut(s) 569, 644
Bse1I ACTGG 3 cut(s) 434, 1033, 1777
Bse3DI GCAATG 1 cut(s) 938
Bse8I GATNNNNATC 1 cut(s) 3078
BseAI TCCGGA 1 cut(s) 2821
BseBI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BseDI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BseGI GGATG 5 cut(s) 208, 1447, 1851, 2608, 2964
BseJI GATNNNNATC 1 cut(s) 3078
BseLI CCNNNNNNNGG 8 cut(s) 440, 1300, 1463, 1721, 1747, 1934, 2828, 3084
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 5 cut(s) 504, 800, 1809, 2110, 2477
BseNI ACTGG 3 cut(s) 434, 1033, 1777
BseRI GAGGAG 2 cut(s) 1316, 3002
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BseYI CCCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 1030
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 9 cut(s) 720, 792, 937, 1032, 1374, 1655, 1714, 2203, 2318
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 519, 2464
BsiHKCI CYCGRG 2 cut(s) 518, 1657
BsiSI CCGG 3 cut(s) 570, 645, 2822
BslFI GGGAC 2 cut(s) 1142, 1168
BslI CCNNNNNNNGG 8 cut(s) 440, 1300, 1463, 1721, 1747, 1934, 2828, 3084
BsmAI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BsmBI CGTCTC 1 cut(s) 2965
BsmFI GGGAC 2 cut(s) 1142, 1168
BsmI GAATGC 2 cut(s) 2026, 2260
BsnI GGCC 9 cut(s) 720, 792, 937, 1032, 1374, 1655, 1714, 2203, 2318
Bso31I GGTCTC 2 cut(s) 2753, 3085
BsoBI CYCGRG 2 cut(s) 518, 1657
Bsp119I TTCGAA 1 cut(s) 988
Bsp1286I GDGCHC 3 cut(s) 519, 1237, 2464
Bsp13I TCCGGA 1 cut(s) 2821
Bsp1720I GCTNAGC 1 cut(s) 2463
Bsp19I CCATGG 1 cut(s) 1457
BspACI CCGC 2 cut(s) 391, 1604
BspANI GGCC 9 cut(s) 720, 792, 937, 1032, 1374, 1655, 1714, 2203, 2318
BspCNI CTCAG 5 cut(s) 505, 801, 1808, 2109, 2476
BspEI TCCGGA 1 cut(s) 2821
BspFNI CGCG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 3025
BspLI GGNNCC 2 cut(s) 887, 1849
BspMAI CTGCAG 1 cut(s) 2855
BspMI ACCTGC 2 cut(s) 2015, 2858
BspPI GGATC 6 cut(s) 23, 98, 324, 1784, 3030, 3068
BspQI GCTCTTC 1 cut(s) 604
BspT104I TTCGAA 1 cut(s) 988
BspTNI GGTCTC 2 cut(s) 2753, 3085
BsrDI GCAATG 1 cut(s) 938
BsrFI RCCGGY 2 cut(s) 569, 644
BsrI ACTGG 3 cut(s) 434, 1033, 1777
BssAI RCCGGY 2 cut(s) 569, 644
BssECI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BssT1I CCWWGG 4 cut(s) 23, 753, 1294, 1457
Bst2UI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
Bst4CI ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
Bst6I CTCTTC 3 cut(s) 604, 2490, 2975
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 9 cut(s) 55, 244, 369, 450, 718, 722, 1871, 2201, 2501
BstDEI CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
BstDSI CCRYGG 1 cut(s) 1457
BstENI CCTNNNNNAGG 1 cut(s) 1298
BstF5I GGATG 5 cut(s) 208, 1447, 1851, 2608, 2964
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 300
BstMAI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BstMWI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
BstNI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BstSCI CCNGG 5 cut(s) 696, 1650, 1978, 2313, 3083
BstSFI CTRYAG 2 cut(s) 1247, 2851
BstSLI GKGCMC 1 cut(s) 1237
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BstV2I GAAGAC 2 cut(s) 1170, 2914
BstX2I RGATCY 1 cut(s) 3073
BstXI CCANNNNNNTGG 2 cut(s) 203, 860
BstYI RGATCY 1 cut(s) 3073
BsuI GTATCC 2 cut(s) 1610, 2090
BsuRI GGCC 9 cut(s) 720, 792, 937, 1032, 1374, 1655, 1714, 2203, 2318
BtgI CCRYGG 1 cut(s) 1457
BtrI CACGTC 2 cut(s) 1182, 2975
BtsCI GGATG 5 cut(s) 208, 1447, 1851, 2608, 2964
BtsI GCAGTG 1 cut(s) 2117
BtsIMutI CAGTG 4 cut(s) 505, 1026, 1094, 2117
BveI ACCTGC 2 cut(s) 2015, 2858
Cac8I GCNNGC 9 cut(s) 55, 244, 369, 450, 718, 722, 1871, 2201, 2501
CaiI CAGNNNCTG 3 cut(s) 773, 1160, 3040
CciI TCATGA 1 cut(s) 3025
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 2 cut(s) 569, 644
Cfr13I GGNCC 3 cut(s) 174, 431, 935
Csp6I GTAC 3 cut(s) 234, 473, 3047
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviQI GTAC 3 cut(s) 234, 473, 3047
DdeI CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
DrdI GACNNNNNNGTC 1 cut(s) 2780
DseDI GACNNNNNNGTC 1 cut(s) 2780
EaeI YGGCCR 1 cut(s) 790
Eam1104I CTCTTC 3 cut(s) 604, 2490, 2975
EarI CTCTTC 3 cut(s) 604, 2490, 2975
Ecl136II GAGCTC 2 cut(s) 517, 2462
Eco130I CCWWGG 4 cut(s) 23, 753, 1294, 1457
Eco147I AGGCCT 2 cut(s) 1655, 2203
Eco24I GRGCYC 2 cut(s) 519, 2464
Eco31I GGTCTC 2 cut(s) 2753, 3085
Eco47I GGWCC 2 cut(s) 174, 431
Eco53kI GAGCTC 2 cut(s) 517, 2462
Eco57I CTGAAG 4 cut(s) 1182, 1737, 2460, 2471
Eco88I CYCGRG 2 cut(s) 518, 1657
EcoICRI GAGCTC 2 cut(s) 517, 2462
EcoNI CCTNNNNNAGG 1 cut(s) 1298
EcoRII CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
EcoT14I CCWWGG 4 cut(s) 23, 753, 1294, 1457
EcoT38I GRGCYC 2 cut(s) 519, 2464
ErhI CCWWGG 4 cut(s) 23, 753, 1294, 1457
Esp3I CGTCTC 1 cut(s) 2965
FaqI GGGAC 2 cut(s) 1142, 1168
FauI CCCGC 1 cut(s) 1611
FblI GTMKAC 2 cut(s) 895, 2137
FokI GGATG 5 cut(s) 215, 1454, 1838, 2615, 2971
FriOI GRGCYC 2 cut(s) 519, 2464
FspBI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
GlaI GCGC 1 cut(s) 299
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 3 cut(s) 668, 1536, 3062
HaeIII GGCC 9 cut(s) 720, 792, 937, 1032, 1374, 1655, 1714, 2203, 2318
HapII CCGG 3 cut(s) 570, 645, 2822
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 896, 2841
HindII GTYRAC 2 cut(s) 896, 2841
HindIII AAGCTT 2 cut(s) 1963, 2548
HpaI GTTAAC 1 cut(s) 2841
HpaII CCGG 3 cut(s) 570, 645, 2822
HphI GGTGA 9 cut(s) 244, 515, 559, 634, 875, 1258, 1310, 2580, 2870
Hpy166II GTNNAC 9 cut(s) 866, 896, 1222, 1492, 2138, 2353, 2414, 2841, 2866
Hpy8I GTNNAC 9 cut(s) 866, 896, 1222, 1492, 2138, 2353, 2414, 2841, 2866
HpyAV CCTTC 7 cut(s) 650, 954, 1094, 1680, 1866, 1928, 1964
HpyCH4III ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
HpyCH4IV ACGT 4 cut(s) 1181, 1817, 2837, 2974
HpyF10VI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
HpyF3I CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
HpySE526I ACGT 4 cut(s) 1181, 1817, 2837, 2974
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 2821
KspAI GTTAAC 1 cut(s) 2841
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 4 cut(s) 757, 1743, 1755, 2422
Lsp1109I GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
LweI GCATC 2 cut(s) 73, 2237
MaeI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
MaeII ACGT 4 cut(s) 1181, 1817, 2837, 2974
MaeIII GTNAC 4 cut(s) 250, 2027, 2089, 2782
MfeI CAATTG 4 cut(s) 326, 667, 689, 2130
MflI RGATCY 1 cut(s) 3073
MhlI GDGCHC 3 cut(s) 519, 1237, 2464
MlsI TGGCCA 1 cut(s) 792
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 5 cut(s) 258, 286, 819, 1929, 2604
MmeI TCCRAC 5 cut(s) 1389, 1702, 1800, 2051, 2779
Mox20I TGGCCA 1 cut(s) 792
MroI TCCGGA 1 cut(s) 2821
MroXI GAANNNNTTC 3 cut(s) 292, 638, 994
MscI TGGCCA 1 cut(s) 792
MslI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
Msp20I TGGCCA 1 cut(s) 792
MspA1I CMGCKG 2 cut(s) 239, 322
MspI CCGG 3 cut(s) 570, 645, 2822
MspR9I CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
MunI CAATTG 4 cut(s) 326, 667, 689, 2130
Mva1269I GAATGC 2 cut(s) 2026, 2260
MvaI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
NcoI CCATGG 1 cut(s) 1457
NlaIV GGNNCC 2 cut(s) 887, 1849
NmuCI GTSAC 3 cut(s) 250, 2027, 2782
NspV TTCGAA 1 cut(s) 988
PaeR7I CTCGAG 1 cut(s) 1657
PagI TCATGA 1 cut(s) 3025
PceI AGGCCT 2 cut(s) 1655, 2203
PciSI GCTCTTC 1 cut(s) 604
PctI GAATGC 2 cut(s) 2026, 2260
PdmI GAANNNNTTC 3 cut(s) 292, 638, 994
PfeI GAWTC 6 cut(s) 346, 731, 1306, 1432, 3002, 3135
PflMI CCANNNNNTGG 2 cut(s) 1463, 1747
PinAI ACCGGT 1 cut(s) 569
PleI GAGTC 5 cut(s) 257, 285, 819, 1928, 2604
PpsI GAGTC 5 cut(s) 257, 285, 819, 1928, 2604
PshBI ATTAAT 1 cut(s) 1698
Psp124BI GAGCTC 2 cut(s) 519, 2464
Psp1406I AACGTT 1 cut(s) 1817
Psp6I CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
PspN4I GGNNCC 2 cut(s) 887, 1849
PspPI GGNCC 3 cut(s) 174, 431, 935
PspXI VCTCGAGB 1 cut(s) 1657
PstI CTGCAG 1 cut(s) 2855
PstNI CAGNNNCTG 3 cut(s) 773, 1160, 3040
PsuI RGATCY 1 cut(s) 3073
PvuII CAGCTG 2 cut(s) 239, 322
RsaI GTAC 3 cut(s) 235, 474, 3048
RsaNI GTAC 3 cut(s) 234, 473, 3047
RseI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
SacI GAGCTC 2 cut(s) 519, 2464
SalI GTCGAC 1 cut(s) 894
SapI GCTCTTC 1 cut(s) 604
Sau96I GGNCC 3 cut(s) 174, 431, 935
SbfI CCTGCAGG 1 cut(s) 2855
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 5 cut(s) 258, 286, 819, 1929, 2604
ScrFI CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
SdaI CCTGCAGG 1 cut(s) 2855
SduI GDGCHC 3 cut(s) 519, 1237, 2464
SfaNI GCATC 2 cut(s) 73, 2237
SfcI CTRYAG 2 cut(s) 1247, 2851
Sfr274I CTCGAG 1 cut(s) 1657
SfuI TTCGAA 1 cut(s) 988
SinI GGWCC 2 cut(s) 174, 431
SlaI CTCGAG 1 cut(s) 1657
SmiMI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
Sse8387I CCTGCAGG 1 cut(s) 2855
SseBI AGGCCT 2 cut(s) 1655, 2203
SsiI CCGC 2 cut(s) 391, 1604
SspI AATATT 2 cut(s) 1998, 2674
SspMI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
SstI GAGCTC 2 cut(s) 519, 2464
StuI AGGCCT 2 cut(s) 1655, 2203
StyD4I CCNGG 5 cut(s) 696, 1650, 1978, 2313, 3083
StyI CCWWGG 4 cut(s) 23, 753, 1294, 1457
TaaI ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
TaiI ACGT 4 cut(s) 1184, 1820, 2840, 2977
TaqI TCGA 7 cut(s) 895, 973, 988, 1573, 1658, 2683, 3102
TatI WGTACW 1 cut(s) 472
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 6 cut(s) 346, 731, 1306, 1432, 3002, 3135
TscAI CASTG 4 cut(s) 505, 1033, 1101, 2124
TseFI GTSAC 3 cut(s) 250, 2027, 2782
TseI GCWGC 9 cut(s) 192, 239, 322, 394, 760, 2384, 2563, 2630, 3034
Tsp45I GTSAC 3 cut(s) 250, 2027, 2782
TspDTI ATGAA 9 cut(s) 22, 552, 602, 699, 1188, 1848, 1947, 2583, 2994
TspGWI ACGGA 1 cut(s) 2652
TspRI CASTG 4 cut(s) 505, 1033, 1101, 2124
Van91I CCANNNNNTGG 2 cut(s) 1463, 1747
VpaK11BI GGWCC 2 cut(s) 174, 431
VspI ATTAAT 1 cut(s) 1698
XagI CCTNNNNNAGG 1 cut(s) 1298
XapI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
XbaI TCTAGA 2 cut(s) 821, 2710
XhoI CTCGAG 1 cut(s) 1657
XmiI GTMKAC 2 cut(s) 895, 2137
XmnI GAANNNNTTC 3 cut(s) 292, 638, 994
XspI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.