Rh5CG256400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010431
Physical Location & Seq
Reverse (-)
27179460 .. 27183851
4392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG256400.1

Sequence Viewer

Length: 3156 bp
ATGGTGGTGCTGGGGATCATTCCAAGGACATTCCTCAAATGGGTTTTCTTGGCTTGCTTTCTTGGTTCTTCTTTGGGTCTTGATGCCCCAATCCAATCCTGTTATCCAAGTGATCTTCTTGCACTCAAGGGGTTTGCAGGAAACCTTACAAGTGGGTCTATTATCACAGCATGGTCCAAGAACTCAAATTGCTGCCAATGGGATGGTGTGGTTTGTGAGAGTGTGAATAATGGTACAGCTGCAAGCAGAGTCACCAAGTTGTTTCTTCCTAACATGAGTCTCAATGGAACAATTTTGCGCTCTTTGGGTGGATTGGATCAGCTGCAATTGCTTAATCTTTCTCTGAATCATCTTGGAGGTGGATTGCCAGCAGAGTTCTCAAACTTGAAGCGGCTGCAAGTTCTTGATTTGAGCTACAACAGGCTTTCAGGACCAGTTTCAGGTGTGCTTGCTAGTTTGAAGTCCATTAAAGTACTGAACATTTCAAGCAATTCAATCAGTGGTGACTTATCTGAGCTCGGGGGTTTCTCAAATCTTGTGGTGTTCAACATAAGCAATAATTCATTCACCGGTCAGTTCAATCCTCAAATTTGTAGCTCTTCCAATGCAATTCATATTCTTGATATGTCTTTGAACCATTTCACCGGCAGTCTTGAAGGCTTAGGCAATTGTAGCACCTCTCTCCAGCAATTGCACCTGGACTTCAATTCATTTGCAGGCCAGCTACCTGAATCTTTGTATTCCTTTTCGGCCTTGGAGCAGCTTTCAGTCTCTGGGAACTCTCTTTCTGGCCAGATAAGCAACAAACTGAGTAAGTTCTCTAGACTCAAGACATTACTCATTTGTGGAAACCAATTTGTTGGTGAACTTCCAAATGTTTTTGGGAACCTTAGTCGACTAGAACAGTTAGTTGCACATTCAAATTTTTTATCTGGGCCATTGCCTTCAACTTTGGCACTCTGCTCAAATCTTCGAGTGCTTGATCTTCGAAACAATTCTCTATCAGGTTCTATTGATCTTAATTTCACTGGCCTTTCCAAACTCTACACCCTTGACCTTGCTACTAATCATTTTTCGGGTTTCCTTCCAAATTCACTGTCATATTGTCGTGAGTTGAAAACCGTAAGCCTTGCCAAGAATGAGTTAAGGGGTTCAGTCCCTGAAGACTTTGCCAAACTCACATCCCTATCCTTTCTTTCATTGTCAAATAACAGTTTTGTAAACTTGTCTGGGGCACTATCTGTGCTACAGAAGTGCAAAAACCTCACCATTCTCATTCTTACCAAAAATTTCCTTGGTGAGGAGATTCCCAAAAATGCAAGTGGCTTTGAAAGGTTAATGGTTTTAGCACTTGGAAATTCTGCTCTTAAAGGCCAAATTCCAGCTTGGTTATTGAGTTGCAAGAAGTTGGAAGTCCTTGATTTGTCTTGGAATCACTTGGATGGCAATATCCCTCCATGGATTGGTCAGATGGAGAACTTGTTTTATGTGGACTTTTCAAATAATTCTCTCTCTGGAGAAATCCCAAAAGGTCTAACAGAGCTGAAAAGCCTCATTTCTACAAATAGCAGTCGATCATATCTTACTGCCTCTGCTGGTATCCCGCTATTTGTGAAGCGAAATAAGAGTGCTAATGGACTGCAATACAACCAGGCCTCGAGTTTTCCACCTTCAATATACTTGAGCAATAATAGAATTAATGGAACAATCTGGCCTGAAGTCGGACGACTAAAACAGCTCCATGTTTTGGATTGGAGCAGGAACAACATTACTGGGATCATTCCTAGCTCAATCTCAGAGATGGAGAACTTGGAAACGTTGGATTTATCATGGAATAATCTGTATGGTTCCATCCCTTCATCACTTAGCAAGCTCACATTCTTGTCAAAGTTTAGTGTGGCAAATAATCACTTGCATGGAGTCATTCCCAATGAAGGACAGTTCTTGAGCTTTCCCAACTCAAGCTTTGAAGGCAACCCAGGACTTTGTGGGGAAATATTTTCTCCATGTAATGCCAAGAATGCAGGTCACAAGCCTGTAATGCCATCAAGTTCAAGCAGTAGATTTGGTCGGAACAGTATCCTTTTAGTAACACTCAGCATAGGAGTTGGGATTGCACTGCTTCTTGCAATTGGTCTACTTAGAATGTCAAGGAGGGAAGCTGAAAATCAAATTGATGATTTTGATGAGGAACACAGCAGGCCTCACAGGTTATCAGAAGCACTTGCATCTTCAAAATTGGTGCTTTTTCAGAATGCAAGTTGTAGGGATTTCACAGTTGCAGAGTTGTTAAAATCTACAAACAATTTTAACCAGGCCAACATAATTGGTTGTGGTGGTTATGGGCTGGTTTACAAAGCCAACCTACCTAATGGCACAAAAGCTGCAATCAAGAGACTTTCTGGGGAATGTGGACAGATGGAGCGTGAATTTCAAGCTGAAGTGGAAGCCCTCTCAAGAGCTCAGCACAAGAACCTTGTCTCTCTTCAGGGCTACTGCAAGCATGGTAATGACAGGTTGTTAATTTACTCTTACATGGAGAATGGAAGCTTGGATTATTGGCTGCATGAAAGTGTTGATGGGATTTCACCTCTCAAATGGGATGTAAGACTCAAGATAGCTCAAGGTGCTGCTCACGGATTGGCTTACTTGCATAAGGGCTGTCAGCCAAATATTGTCCATCGAGATATAAAAACGAGCAACATTCTTCTAGATGAGAAATTTGAAGCTCATCTGGCTGATTTTGGTCTCTCAAGGCTACTTCGTCCTTATGACACCCATGTCACAACAGATTTAGTTGGAACTTTGGGCTATATTCCTCCGGAGTATAGTCAGACGTTAACTGCAACCTGCAGGGGTGATGTTTACAGTTTTGGTGTTGTTCTTCTTGAACTTCTTACGGGCAGAAGACCTGTTGAAGTGTGCAAAGGGAAAAATTGCAGAGATTTGGTATCATGGATGTTTCAGATGAGACGTGAGAAGAGAGAGGAGGAAATTATAGATTCATCAATCTGGAATAAGGATCATGAGAAGCAGCTTCTGGAGGTACTTGATGTTGCCTGTAAATGCCTAGATCCCAATCCCAGGCAGAGACCCTTTATTGAAGAAGTTGTATCATGGCTTGATGGTATTGGATTCCAGAGTGAGAAACAATGA

Protein Analysis

1051

Amino Acids

115.25

Weight (kDa)

7.73

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 72 5.8e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 107 - 166 3e-06 Leucine rich repeat
LRR_8 PF13855 322 - 382 4.6e-06 Leucine rich repeat
LRR_8 PF13855 347 - 405 1.9e-07 Leucine rich repeat
LRR_8 PF13855 421 - 480 3.1e-06 Leucine rich repeat
LRR_8 PF13855 558 - 614 2.3e-07 Leucine rich repeat
Pkinase PF00069 771 - 1038 2.2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 773 - 1039 3.6e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2780
Acc36I ACCTGC 2 cut(s) 2015, 2858
AccB7I CCANNNNNTGG 2 cut(s) 1463, 1747
AccI GTMKAC 2 cut(s) 895, 2137
AccIII TCCGGA 1 cut(s) 2821
AciI CCGC 2 cut(s) 391, 1604
AclI AACGTT 1 cut(s) 1817
AclWI GGATC 5 cut(s) 23, 324, 1784, 3030, 3068
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
AcuI CTGAAG 4 cut(s) 1182, 1737, 2460, 2471
AfaI GTAC 3 cut(s) 235, 474, 3048
AfiI CCNNNNNNNGG 8 cut(s) 40, 440, 1300, 1463, 1721, 1747, 1934, 3084
AgeI ACCGGT 1 cut(s) 569
AjiI CACGTC 1 cut(s) 2975
AjnI CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
AjuI GAANNNNNNNTTGG 2 cut(s) 2534, 2566
Alw21I GWGCWC 2 cut(s) 519, 2464
Alw26I GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
AlwI GGATC 5 cut(s) 23, 324, 1784, 3030, 3068
AlwNI CAGNNNCTG 3 cut(s) 773, 1160, 3040
Ama87I CYCGRG 2 cut(s) 518, 1657
Aor13HI TCCGGA 1 cut(s) 2821
ApeKI GCWGC 9 cut(s) 192, 239, 322, 394, 760, 2384, 2563, 2630, 3034
ApoI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
ArsI GACNNNNNNTTYG 2 cut(s) 1082, 1114
AseI ATTAAT 1 cut(s) 1698
AsiGI ACCGGT 1 cut(s) 569
Asp700I GAANNNNTTC 2 cut(s) 638, 994
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 174, 431, 935
AsuHPI GGTGA 9 cut(s) 244, 515, 559, 634, 875, 1258, 1310, 2580, 2870
AsuII TTCGAA 1 cut(s) 988
AvaI CYCGRG 2 cut(s) 518, 1657
AvaII GGWCC 2 cut(s) 174, 431
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 792
BanII GRGCYC 2 cut(s) 519, 2464
BbsI GAAGAC 2 cut(s) 1170, 2914
Bbv12I GWGCWC 2 cut(s) 519, 2464
BbvI GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BciT130I CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BciVI GTATCC 2 cut(s) 1610, 2090
BcoDI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BfaI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
BfmI CTRYAG 2 cut(s) 1247, 2851
BfuAI ACCTGC 2 cut(s) 2015, 2858
BfuI GTATCC 2 cut(s) 1610, 2090
BlpI GCTNAGC 1 cut(s) 2463
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
Bme18I GGWCC 2 cut(s) 174, 431
BmeT110I CYCGRG 2 cut(s) 518, 1657
BmgBI CACGTC 1 cut(s) 2975
BmgT120I GGNCC 3 cut(s) 174, 431, 935
BmiI GGNNCC 2 cut(s) 887, 1849
BmrFI CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BmrI ACTGGG 1 cut(s) 1782
BmsI GCATC 2 cut(s) 73, 2237
BmuI ACTGGG 1 cut(s) 1782
BpiI GAAGAC 2 cut(s) 1170, 2914
BpmI CTGGAG 3 cut(s) 668, 1536, 3062
Bpu10I CCTNAGC 1 cut(s) 661
Bpu1102I GCTNAGC 1 cut(s) 2463
Bpu14I TTCGAA 1 cut(s) 988
BpuEI CTTGAG 9 cut(s) 110, 812, 1702, 1945, 1966, 2440, 2597, 2607, 2737
BsaBI GATNNNNATC 1 cut(s) 3078
BsaI GGTCTC 2 cut(s) 2753, 3085
BsaJI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BsaWI WCCGGW 2 cut(s) 569, 2821
BsaXI ACNNNNNCTCC 2 cut(s) 1466, 1496
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 440, 1300, 1463, 1721, 1747, 1934, 3084
Bse118I RCCGGY 2 cut(s) 569, 644
Bse1I ACTGG 3 cut(s) 434, 1033, 1777
Bse3DI GCAATG 1 cut(s) 938
Bse8I GATNNNNATC 1 cut(s) 3078
BseAI TCCGGA 1 cut(s) 2821
BseBI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BseDI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BseGI GGATG 6 cut(s) 208, 1181, 1447, 1851, 2608, 2964
BseJI GATNNNNATC 1 cut(s) 3078
BseLI CCNNNNNNNGG 8 cut(s) 40, 440, 1300, 1463, 1721, 1747, 1934, 3084
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 5 cut(s) 504, 800, 1809, 2110, 2477
BseNI ACTGG 3 cut(s) 434, 1033, 1777
BseRI GAGGAG 2 cut(s) 1316, 3002
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BseYI CCCAGC 1 cut(s) 10
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 519, 2464
BsiHKCI CYCGRG 2 cut(s) 518, 1657
BsiSI CCGG 3 cut(s) 570, 645, 2822
BslFI GGGAC 1 cut(s) 1142
BslI CCNNNNNNNGG 8 cut(s) 40, 440, 1300, 1463, 1721, 1747, 1934, 3084
BsmAI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BsmBI CGTCTC 1 cut(s) 2965
BsmFI GGGAC 1 cut(s) 1142
BsmI GAATGC 2 cut(s) 2026, 2260
Bso31I GGTCTC 2 cut(s) 2753, 3085
BsoBI CYCGRG 2 cut(s) 518, 1657
Bsp119I TTCGAA 1 cut(s) 988
Bsp1286I GDGCHC 3 cut(s) 519, 1237, 2464
Bsp13I TCCGGA 1 cut(s) 2821
Bsp143I GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
Bsp1720I GCTNAGC 1 cut(s) 2463
Bsp19I CCATGG 1 cut(s) 1457
BspACI CCGC 2 cut(s) 391, 1604
BspCNI CTCAG 5 cut(s) 505, 801, 1808, 2109, 2476
BspEI TCCGGA 1 cut(s) 2821
BspHI TCATGA 1 cut(s) 3025
BspLI GGNNCC 2 cut(s) 887, 1849
BspMAI CTGCAG 1 cut(s) 2855
BspMI ACCTGC 2 cut(s) 2015, 2858
BspPI GGATC 5 cut(s) 23, 324, 1784, 3030, 3068
BspQI GCTCTTC 1 cut(s) 604
BspT104I TTCGAA 1 cut(s) 988
BspTNI GGTCTC 2 cut(s) 2753, 3085
BsrDI GCAATG 1 cut(s) 938
BsrFI RCCGGY 2 cut(s) 569, 644
BsrI ACTGG 3 cut(s) 434, 1033, 1777
BssAI RCCGGY 2 cut(s) 569, 644
BssECI CCNNGG 6 cut(s) 23, 753, 1294, 1457, 1978, 3083
BssMI GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
BssT1I CCWWGG 4 cut(s) 23, 753, 1294, 1457
Bst2UI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
Bst4CI ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
Bst6I CTCTTC 3 cut(s) 604, 2490, 2975
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 9 cut(s) 55, 244, 369, 450, 718, 722, 1871, 2201, 2501
BstDEI CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
BstDSI CCRYGG 1 cut(s) 1457
BstENI CCTNNNNNAGG 1 cut(s) 1298
BstF5I GGATG 6 cut(s) 208, 1181, 1447, 1851, 2608, 2964
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 9 cut(s) 18, 115, 319, 985, 1018, 1577, 1779, 3025, 3076
BstMAI GTCTC 7 cut(s) 284, 775, 2389, 2485, 2753, 2965, 3085
BstMBI GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
BstMWI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
BstNI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
BstSCI CCNGG 5 cut(s) 696, 1650, 1978, 2313, 3083
BstSFI CTRYAG 2 cut(s) 1247, 2851
BstSLI GKGCMC 1 cut(s) 1237
BstV1I GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
BstV2I GAAGAC 2 cut(s) 1170, 2914
BstX2I RGATCY 1 cut(s) 3073
BstXI CCANNNNNNTGG 2 cut(s) 203, 860
BstYI RGATCY 1 cut(s) 3073
BsuI GTATCC 2 cut(s) 1610, 2090
BtgI CCRYGG 1 cut(s) 1457
BtrI CACGTC 1 cut(s) 2975
BtsCI GGATG 6 cut(s) 208, 1181, 1447, 1851, 2608, 2964
BtsI GCAGTG 1 cut(s) 2117
BtsIMutI CAGTG 4 cut(s) 505, 1026, 1094, 2117
BveI ACCTGC 2 cut(s) 2015, 2858
Cac8I GCNNGC 9 cut(s) 55, 244, 369, 450, 718, 722, 1871, 2201, 2501
CaiI CAGNNNCTG 3 cut(s) 773, 1160, 3040
CciI TCATGA 1 cut(s) 3025
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 2 cut(s) 569, 644
Cfr13I GGNCC 3 cut(s) 174, 431, 935
Csp6I GTAC 3 cut(s) 234, 473, 3047
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviQI GTAC 3 cut(s) 234, 473, 3047
DdeI CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
DpnI GATC 9 cut(s) 17, 114, 318, 984, 1017, 1576, 1778, 3024, 3075
DpnII GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
DrdI GACNNNNNNGTC 1 cut(s) 2780
DseDI GACNNNNNNGTC 1 cut(s) 2780
EaeI YGGCCR 1 cut(s) 790
Eam1104I CTCTTC 3 cut(s) 604, 2490, 2975
EarI CTCTTC 3 cut(s) 604, 2490, 2975
Ecl136II GAGCTC 2 cut(s) 517, 2462
Eco130I CCWWGG 4 cut(s) 23, 753, 1294, 1457
Eco147I AGGCCT 2 cut(s) 1655, 2203
Eco24I GRGCYC 2 cut(s) 519, 2464
Eco31I GGTCTC 2 cut(s) 2753, 3085
Eco47I GGWCC 2 cut(s) 174, 431
Eco53kI GAGCTC 2 cut(s) 517, 2462
Eco57I CTGAAG 4 cut(s) 1182, 1737, 2460, 2471
Eco88I CYCGRG 2 cut(s) 518, 1657
EcoICRI GAGCTC 2 cut(s) 517, 2462
EcoNI CCTNNNNNAGG 1 cut(s) 1298
EcoRII CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
EcoT14I CCWWGG 4 cut(s) 23, 753, 1294, 1457
EcoT38I GRGCYC 2 cut(s) 519, 2464
ErhI CCWWGG 4 cut(s) 23, 753, 1294, 1457
Esp3I CGTCTC 1 cut(s) 2965
FaqI GGGAC 1 cut(s) 1142
FauI CCCGC 1 cut(s) 1611
FblI GTMKAC 2 cut(s) 895, 2137
FokI GGATG 6 cut(s) 215, 1168, 1454, 1838, 2615, 2971
FriOI GRGCYC 2 cut(s) 519, 2464
FspBI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
GlaI GCGC 1 cut(s) 299
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 3 cut(s) 668, 1536, 3062
HapII CCGG 3 cut(s) 570, 645, 2822
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 896, 2841
HindII GTYRAC 2 cut(s) 896, 2841
HindIII AAGCTT 2 cut(s) 1963, 2548
HpaI GTTAAC 1 cut(s) 2841
HpaII CCGG 3 cut(s) 570, 645, 2822
HphI GGTGA 9 cut(s) 244, 515, 559, 634, 875, 1258, 1310, 2580, 2870
Hpy166II GTNNAC 9 cut(s) 866, 896, 1222, 1492, 2138, 2353, 2414, 2841, 2866
Hpy8I GTNNAC 9 cut(s) 866, 896, 1222, 1492, 2138, 2353, 2414, 2841, 2866
HpyAV CCTTC 7 cut(s) 650, 954, 1094, 1680, 1866, 1928, 1964
HpyCH4III ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
HpyCH4IV ACGT 3 cut(s) 1817, 2837, 2974
HpyF10VI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
HpyF3I CTNAG 9 cut(s) 513, 661, 809, 890, 1795, 1865, 2096, 2141, 2463
HpySE526I ACGT 3 cut(s) 1817, 2837, 2974
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 2821
KspAI GTTAAC 1 cut(s) 2841
Kzo9I GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 4 cut(s) 757, 1743, 1755, 2422
Lsp1109I GCAGC 9 cut(s) 179, 226, 309, 381, 772, 2371, 2550, 2617, 3046
LweI GCATC 2 cut(s) 73, 2237
MaeI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
MaeII ACGT 3 cut(s) 1817, 2837, 2974
MaeIII GTNAC 5 cut(s) 250, 503, 2027, 2089, 2782
MalI GATC 9 cut(s) 17, 114, 318, 984, 1017, 1576, 1778, 3024, 3075
MboI GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
MfeI CAATTG 4 cut(s) 326, 667, 689, 2130
MflI RGATCY 1 cut(s) 3073
MhlI GDGCHC 3 cut(s) 519, 1237, 2464
MlsI TGGCCA 1 cut(s) 792
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 5 cut(s) 258, 286, 819, 1929, 2604
MmeI TCCRAC 5 cut(s) 1389, 1702, 1800, 2051, 2779
Mox20I TGGCCA 1 cut(s) 792
MroI TCCGGA 1 cut(s) 2821
MroXI GAANNNNTTC 2 cut(s) 638, 994
MscI TGGCCA 1 cut(s) 792
MslI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
Msp20I TGGCCA 1 cut(s) 792
MspA1I CMGCKG 2 cut(s) 239, 322
MspI CCGG 3 cut(s) 570, 645, 2822
MspR9I CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
MunI CAATTG 4 cut(s) 326, 667, 689, 2130
Mva1269I GAATGC 2 cut(s) 2026, 2260
MvaI CCWGG 5 cut(s) 698, 1652, 1980, 2315, 3085
MwoI GCNNNNNNNGC 8 cut(s) 328, 672, 798, 1971, 2021, 2041, 2627, 2735
NcoI CCATGG 1 cut(s) 1457
NdeII GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
NlaIV GGNNCC 2 cut(s) 887, 1849
NmuCI GTSAC 4 cut(s) 250, 503, 2027, 2782
NspV TTCGAA 1 cut(s) 988
PaeR7I CTCGAG 1 cut(s) 1657
PagI TCATGA 1 cut(s) 3025
PceI AGGCCT 2 cut(s) 1655, 2203
PciSI GCTCTTC 1 cut(s) 604
PctI GAATGC 2 cut(s) 2026, 2260
PdmI GAANNNNTTC 2 cut(s) 638, 994
PfeI GAWTC 6 cut(s) 346, 731, 1306, 1432, 3002, 3135
PflMI CCANNNNNTGG 2 cut(s) 1463, 1747
PinAI ACCGGT 1 cut(s) 569
PleI GAGTC 5 cut(s) 257, 285, 819, 1928, 2604
PpsI GAGTC 5 cut(s) 257, 285, 819, 1928, 2604
PshBI ATTAAT 1 cut(s) 1698
Psp124BI GAGCTC 2 cut(s) 519, 2464
Psp1406I AACGTT 1 cut(s) 1817
Psp6I CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 5 cut(s) 696, 1650, 1978, 2313, 3083
PspN4I GGNNCC 2 cut(s) 887, 1849
PspPI GGNCC 3 cut(s) 174, 431, 935
PspXI VCTCGAGB 1 cut(s) 1657
PstI CTGCAG 1 cut(s) 2855
PstNI CAGNNNCTG 3 cut(s) 773, 1160, 3040
PsuI RGATCY 1 cut(s) 3073
PvuII CAGCTG 2 cut(s) 239, 322
RsaI GTAC 3 cut(s) 235, 474, 3048
RsaNI GTAC 3 cut(s) 234, 473, 3047
RseI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
SacI GAGCTC 2 cut(s) 519, 2464
SalI GTCGAC 1 cut(s) 894
SapI GCTCTTC 1 cut(s) 604
Sau3AI GATC 9 cut(s) 15, 112, 316, 982, 1015, 1574, 1776, 3022, 3073
Sau96I GGNCC 3 cut(s) 174, 431, 935
SbfI CCTGCAGG 1 cut(s) 2855
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 5 cut(s) 258, 286, 819, 1929, 2604
ScrFI CCNGG 5 cut(s) 698, 1652, 1980, 2315, 3085
SdaI CCTGCAGG 1 cut(s) 2855
SduI GDGCHC 3 cut(s) 519, 1237, 2464
SfaNI GCATC 2 cut(s) 73, 2237
SfcI CTRYAG 2 cut(s) 1247, 2851
Sfr274I CTCGAG 1 cut(s) 1657
SfuI TTCGAA 1 cut(s) 988
SinI GGWCC 2 cut(s) 174, 431
SlaI CTCGAG 1 cut(s) 1657
SmiMI CAYNNNNRTG 4 cut(s) 1440, 1914, 2508, 2571
Sse8387I CCTGCAGG 1 cut(s) 2855
SseBI AGGCCT 2 cut(s) 1655, 2203
SsiI CCGC 2 cut(s) 391, 1604
SspI AATATT 2 cut(s) 1998, 2674
SspMI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
SstI GAGCTC 2 cut(s) 519, 2464
StuI AGGCCT 2 cut(s) 1655, 2203
StyD4I CCNGG 5 cut(s) 696, 1650, 1978, 2313, 3083
StyI CCWWGG 4 cut(s) 23, 753, 1294, 1457
TaaI ACNGT 8 cut(s) 906, 1098, 1123, 1214, 1941, 2078, 2278, 2870
TaiI ACGT 3 cut(s) 1820, 2840, 2977
TaqI TCGA 6 cut(s) 895, 973, 988, 1573, 1658, 2683
TatI WGTACW 1 cut(s) 472
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 6 cut(s) 346, 731, 1306, 1432, 3002, 3135
TscAI CASTG 4 cut(s) 505, 1033, 1101, 2124
TseFI GTSAC 4 cut(s) 250, 503, 2027, 2782
TseI GCWGC 9 cut(s) 192, 239, 322, 394, 760, 2384, 2563, 2630, 3034
Tsp45I GTSAC 4 cut(s) 250, 503, 2027, 2782
TspDTI ATGAA 8 cut(s) 552, 602, 699, 1188, 1848, 1947, 2583, 2994
TspGWI ACGGA 1 cut(s) 2652
TspRI CASTG 4 cut(s) 505, 1033, 1101, 2124
Van91I CCANNNNNTGG 2 cut(s) 1463, 1747
VpaK11BI GGWCC 2 cut(s) 174, 431
VspI ATTAAT 1 cut(s) 1698
XagI CCTNNNNNAGG 1 cut(s) 1298
XapI RAATTY 8 cut(s) 588, 922, 1090, 1288, 1357, 1377, 2429, 2720
XbaI TCTAGA 2 cut(s) 821, 2710
XhoI CTCGAG 1 cut(s) 1657
XmiI GTMKAC 2 cut(s) 895, 2137
XmnI GAANNNNTTC 2 cut(s) 638, 994
XspI CTAG 6 cut(s) 453, 822, 899, 1785, 2711, 3071
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.