RchiOBHm_Chr5g0032901
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010431
Physical Location & Seq
Reverse (-)
26703293 .. 26707727
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31203

Sequence Viewer

Length: 3081 bp
ATGGTGGTGCTGGGGATCATTCCAAGGACATTCCTCAAATGGGTTTTCTTGGCTTGCTTTCTTGGTTCTTCTTTGGGTCTTGATGCCCCAATCCAATCCTGTGATCCAAGTGATCTTCTTGCACTCAAGGGGTTTGCAGGAAACCTTGCAAGTGGGTCTATTATCACAGCATGGTCCAAGAACTCAAATTGCTGCCAATGGGATGGTGTGGTTTGTGAGAGTGTGAATAATGGTACAGCTGCAAGCAGAGTCACCAAGTTGTTTCTTCCCAACATGAGTCTCAATGGAACAATTTCGCGCTCTTTGGGTGGATTGGATCAGCTGCAATTGCTTAATCTTTCTCTGAATCATCTTGGAGGTGGGTTGCCAGCAGAGTTCTCAAACTTGAAGCGGCTGCAAGTTCTTGATTTGAGCTACAACAGGCTTTCAGGACCAGTTTCAGGTGTGCTTGCTAGTTTGAAGTCCATTAAAGTACTGAACATTTCAAGCAATTCAATCAGTGGTGACTTATCTGAGCTCGAAGGTTTCTCAAATCTTGTGGTGTTCAACATAAGCAATAATTCATTCACCGGTCATCTTGAAGGCTTAGGCAATTGTAGCACCTCTCTCCAGCAATTGCACCTGGACTTCAATTCATTTGCAGGCCAGCTACCTGAATCTTTGTATTCCTTTTCGGCCTTGGAGCAGCTTTCAGTCTCTGGGAACTCTCTTTCTGGCCAGATAAGCAACAAACTGAGTAAGTTCTCTAGACTCAAGACATTACTCATTTGTGGAAACCAATTTGTTGGTGAACTTCCAAATGTTTTTGGGAACCTTAGTCGACTAGAACAGTTAGTTGCACATTCAAATTTTTTTTCTGGGCCATTGCCTTCAACTTTGGCACTCTGCTCAAATCTTAGGGTGCTTGATCTTCGAAACAATTCTCTATCAGGTTCTATTGATCTTAATTTCACTGGCCTTTCCAAACTCTGCACCCTTGACCTTGCTACTAATCGTTTTTCGGGCTTCCTTCCAAATTCACTGTCATATTGTCGTGAGTTGAAAACCGTAAGCCTTGCCAAGAATGAGTTAAGGGGTTCAGTCCCTGAAGACTTTGCCAAACTCACATCCCTATCCTTTCTTTCATTGTCAAATAACAGTTTTGTAAACTTGTCTGGGGCACTATCTGTGCTACAGAAGTGCAAAAACCTCACCATTCTCATTCTTACCAAAAATTTCCTTGGTGAGGAGATTCCCAAAAATGCAAGTGGCTTTGAAAGTTTAATGGTTTTAGCTCTTGGAAATTCTGCTCTTAAAGGCCAAATTCCAGCTTGGTTATTGAGTTGCAAGAAGTTGGAAGTCCTTGATTTGTCTTGGAATCACTTGGATGGCAATATCCCTCCATGGATTGGTCAGATGGAGAACTTGTTTTATGTGGACTTTTCAAATAATTCTCTCTCTGGAGAAATCCCAAAAGGTCTAACAGAGCTGAAAAGCCTCATTTCTACAAATAGCAGTCGATCATATCTTACTGCCTCTGCTGGTGTCCCGCTATTTGTGAAGCGAAATAAGAGTGCTAATGGACTGCAATACAACCAGGCCTCGAGTTTTCCACCTTCAATATACTTGAGCAATAATAGAATTAATGGAACAATCTGGCCTGAAGTCGGACGACTAAAACAGCTCCATGTTTTGGATTGGAGCAGGAACAACATTACTGGGATCATTCCTAGCTCAATCTCAGAGATGGAGAACTTGGAAACGTTGGATTTATCATGGAATAATCTGTATGGTTCCATCCCTTCATCACTTAGCAAGCTCACATTCTTGTCAAAGTTTAGTGTGGCAAATAATCACTTGCATGGAGTCATTCCCAATGAAGGACAGTTCCTGAGCTTTCCCAACTCAAGCTTTGAAGGCAACCCAGGACTTTGTGGGGAAATATTTTCTCCATGTAATGCCAAGAATGCAGGTCTCAAGCCTGTAATGCCATCAAGTTCAAGCAGTAGATTTGGTCGGAACAGTATCCTTTTAGTAACACTCAGCATAGGAGTTGGGATTGCACTGCTTCTTGCAATTGGTCTACTTAGAATGTCAAGGAGCGAAGCTGAAAATCAAATTGATGATTTTGATGAGGAACACAGCAGGCCTCACAGGTTGTCAGAAGCACTTGCATCTTCAAAATTGGTGCTTTTTCAGAATGCAAGTTGTAGGGATTTCACAGTTGCAGAGTTGTTAAAATCTACAAACAATTTTAACCAGGCCAACATAATTGGTTGCGGTGGTTATGGGCTGGTTTACAAAGCCAACCTACCTAATGGCACAAAAGCTGCAATCAAGAGACTTTCTGGGGAATGTGGACAGATGGAGCGTGAATTTCAAGCTGAAGTGGAAGCCCTCTCAAGAGCTCAGCACAAGAACCTTGTCTCTCTTCAGGGCTACTGCAAGCATGGTAATGACAGGTTGTTAATTTACTCTTACATGGAGAATGGAAGCTTGGATTATTGGCTGCATGAAAGTGTTGATGGGATTTCACCTCTCAAATGGGATGTAAGACTCAAGATAGCTCAAGGTGCTGCTCACGGATTGGCTTACTTGCATAAGGGCTGTCAGCCAAATATTGTCCATCGAGATATAAAAACAAGCAACATTCTTCTAGATGAGAAATTTGAAGCTCATCTGGCTGATTTTGGTCTCTCAAGGCTACTTCGTCCTTATGACACCCATGTCACAACAGATTTAGTTGGAACTTTGGGCTATATTCCTCCAGAGTATAGTCAGACGTTAACTGCAACCTGCAGGGGCGATGTTTACAGTTTTGGTGTTGTTCTTCTTGAACTTCTTACGGGCAGAAGACCTGTTGAAGTGTGCAAAGGGAAAAATTGCAGAGATTTGGTATCATGGATGTTTCAGATGAGACGTGAGAAGAGAGAGGAGGAAATTATAGATTCATCAATCTGGAATAAGGATGATGAGAAGCAGCTTCTGGAGGTACTTGATGTTGCCTGTAAATGCCTAGATCCCAATCCCAGGCAGAGACCCTTTATCGAAGAAGTTGTATCATGGCTTGATGGTATTGGATTGCAGAGTGAGAAACAATGA

Protein Analysis

1026

Amino Acids

112.27

Weight (kDa)

6.99

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 72 9.3e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 98 - 278 2.4e-09 Leucine-rich repeat region
LRR_8 PF13855 297 - 357 7.4e-07 Leucine rich repeat
LRR_8 PF13855 396 - 455 1.5e-06 Leucine rich repeat
LRR_8 PF13855 533 - 589 2.3e-07 Leucine rich repeat
Pkinase PF00069 746 - 1013 2.3e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 748 - 1014 3.8e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2705
Acc36I ACCTGC 2 cut(s) 1940, 2783
AccB7I CCANNNNNTGG 2 cut(s) 1388, 1672
AccI GTMKAC 2 cut(s) 820, 2062
AccII CGCG 1 cut(s) 298
AciI CCGC 3 cut(s) 391, 1529, 2259
AclI AACGTT 1 cut(s) 1742
AclWI GGATC 5 cut(s) 23, 98, 324, 1709, 2993
AcoI YGGCCR 1 cut(s) 715
AcsI RAATTY 7 cut(s) 847, 1015, 1213, 1282, 1302, 2354, 2645
AcuI CTGAAG 4 cut(s) 1107, 1662, 2385, 2396
AfaI GTAC 3 cut(s) 235, 474, 2973
AfiI CCNNNNNNNGG 8 cut(s) 40, 440, 1225, 1388, 1646, 1672, 1859, 3009
AgeI ACCGGT 1 cut(s) 569
AjiI CACGTC 1 cut(s) 2900
AjnI CCWGG 5 cut(s) 621, 1575, 1903, 2238, 3008
AjuI GAANNNNNNNTTGG 2 cut(s) 2459, 2491
Alw21I GWGCWC 2 cut(s) 519, 2389
Alw26I GTCTC 8 cut(s) 284, 700, 1958, 2314, 2410, 2678, 2890, 3010
AlwI GGATC 5 cut(s) 23, 98, 324, 1709, 2993
AlwNI CAGNNNCTG 4 cut(s) 698, 1085, 1870, 2965
Ama87I CYCGRG 1 cut(s) 1582
ApeKI GCWGC 9 cut(s) 192, 239, 322, 394, 685, 2309, 2488, 2555, 2959
ApoI RAATTY 7 cut(s) 847, 1015, 1213, 1282, 1302, 2354, 2645
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
AseI ATTAAT 1 cut(s) 1623
AsiGI ACCGGT 1 cut(s) 569
Asp700I GAANNNNTTC 2 cut(s) 292, 919
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 174, 431, 860
AsuHPI GGTGA 7 cut(s) 244, 515, 559, 800, 1183, 1235, 2505
AsuII TTCGAA 1 cut(s) 913
AvaI CYCGRG 1 cut(s) 1582
AvaII GGWCC 2 cut(s) 174, 431
BaeGI GKGCMC 1 cut(s) 1162
BalI TGGCCA 1 cut(s) 717
BanII GRGCYC 2 cut(s) 519, 2389
BbsI GAAGAC 2 cut(s) 1095, 2839
Bbv12I GWGCWC 2 cut(s) 519, 2389
BbvI GCAGC 9 cut(s) 179, 226, 309, 381, 697, 2296, 2475, 2542, 2971
BciT130I CCWGG 5 cut(s) 623, 1577, 1905, 2240, 3010
BciVI GTATCC 1 cut(s) 2015
BcoDI GTCTC 8 cut(s) 284, 700, 1958, 2314, 2410, 2678, 2890, 3010
BfaI CTAG 6 cut(s) 453, 747, 824, 1710, 2636, 2996
BfmI CTRYAG 2 cut(s) 1172, 2776
BfuAI ACCTGC 2 cut(s) 1940, 2783
BfuI GTATCC 1 cut(s) 2015
BlpI GCTNAGC 1 cut(s) 2388
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 5 cut(s) 623, 1577, 1905, 2240, 3010
Bme18I GGWCC 2 cut(s) 174, 431
BmeT110I CYCGRG 1 cut(s) 1582
BmgBI CACGTC 1 cut(s) 2900
BmgT120I GGNCC 3 cut(s) 174, 431, 860
BmiI GGNNCC 2 cut(s) 812, 1774
BmrFI CCNGG 5 cut(s) 623, 1577, 1905, 2240, 3010
BmrI ACTGGG 1 cut(s) 1707
BmsI GCATC 2 cut(s) 73, 2162
BmuI ACTGGG 1 cut(s) 1707
BpiI GAAGAC 2 cut(s) 1095, 2839
BpmI CTGGAG 4 cut(s) 593, 1461, 2730, 2987
Bpu10I CCTNAGC 2 cut(s) 586, 1871
Bpu1102I GCTNAGC 1 cut(s) 2388
Bpu14I TTCGAA 1 cut(s) 913
BpuEI CTTGAG 9 cut(s) 110, 737, 1627, 1870, 1940, 2365, 2522, 2532, 2662
BsaBI GATNNNNATC 1 cut(s) 3003
BsaI GGTCTC 3 cut(s) 1958, 2678, 3010
BsaJI CCNNGG 6 cut(s) 23, 678, 1219, 1382, 1903, 3008
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 1391, 1421
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 440, 1225, 1388, 1646, 1672, 1859, 3009
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 3 cut(s) 434, 958, 1702
Bse3DI GCAATG 1 cut(s) 863
Bse8I GATNNNNATC 1 cut(s) 3003
BseBI CCWGG 5 cut(s) 623, 1577, 1905, 2240, 3010
BseDI CCNNGG 6 cut(s) 23, 678, 1219, 1382, 1903, 3008
BseGI GGATG 7 cut(s) 208, 1106, 1372, 1776, 2533, 2889, 2953
BseJI GATNNNNATC 1 cut(s) 3003
BseLI CCNNNNNNNGG 8 cut(s) 40, 440, 1225, 1388, 1646, 1672, 1859, 3009
BseMI GCAATG 1 cut(s) 863
BseMII CTCAG 6 cut(s) 504, 725, 1734, 1862, 2035, 2402
BseNI ACTGG 3 cut(s) 434, 958, 1702
BseRI GAGGAG 2 cut(s) 1241, 2927
BseSI GKGCMC 1 cut(s) 1162
BseXI GCAGC 9 cut(s) 179, 226, 309, 381, 697, 2296, 2475, 2542, 2971
BseYI CCCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 955
Bsh1236I CGCG 1 cut(s) 298
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 519, 2389
BsiHKCI CYCGRG 1 cut(s) 1582
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 2 cut(s) 1067, 1511
BslI CCNNNNNNNGG 8 cut(s) 40, 440, 1225, 1388, 1646, 1672, 1859, 3009
BsmAI GTCTC 8 cut(s) 284, 700, 1958, 2314, 2410, 2678, 2890, 3010
BsmBI CGTCTC 1 cut(s) 2890
BsmFI GGGAC 2 cut(s) 1067, 1511
BsmI GAATGC 2 cut(s) 1951, 2185
Bso31I GGTCTC 3 cut(s) 1958, 2678, 3010
BsoBI CYCGRG 1 cut(s) 1582
Bsp119I TTCGAA 1 cut(s) 913
Bsp1286I GDGCHC 3 cut(s) 519, 1162, 2389
Bsp143I GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
Bsp1720I GCTNAGC 1 cut(s) 2388
Bsp19I CCATGG 1 cut(s) 1382
BspACI CCGC 3 cut(s) 391, 1529, 2259
BspCNI CTCAG 6 cut(s) 505, 726, 1733, 1863, 2034, 2401
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 812, 1774
BspMAI CTGCAG 1 cut(s) 2780
BspMI ACCTGC 2 cut(s) 1940, 2783
BspPI GGATC 5 cut(s) 23, 98, 324, 1709, 2993
BspT104I TTCGAA 1 cut(s) 913
BspTNI GGTCTC 3 cut(s) 1958, 2678, 3010
BsrDI GCAATG 1 cut(s) 863
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 3 cut(s) 434, 958, 1702
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 6 cut(s) 23, 678, 1219, 1382, 1903, 3008
BssMI GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
BssT1I CCWWGG 4 cut(s) 23, 678, 1219, 1382
Bst2UI CCWGG 5 cut(s) 623, 1577, 1905, 2240, 3010
Bst4CI ACNGT 8 cut(s) 831, 1023, 1048, 1139, 1866, 2003, 2203, 2795
Bst6I CTCTTC 2 cut(s) 2415, 2900
BstBI TTCGAA 1 cut(s) 913
BstC8I GCNNGC 9 cut(s) 55, 244, 369, 450, 643, 647, 1796, 2126, 2426
BstDSI CCRYGG 1 cut(s) 1382
BstENI CCTNNNNNAGG 1 cut(s) 1223
BstF5I GGATG 7 cut(s) 208, 1106, 1372, 1776, 2533, 2889, 2953
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 9 cut(s) 18, 106, 115, 319, 910, 943, 1502, 1704, 3001
BstMAI GTCTC 8 cut(s) 284, 700, 1958, 2314, 2410, 2678, 2890, 3010
BstMBI GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
BstMWI GCNNNNNNNGC 8 cut(s) 328, 597, 723, 1896, 1946, 1966, 2552, 2660
BstNI CCWGG 5 cut(s) 623, 1577, 1905, 2240, 3010
BstSCI CCNGG 5 cut(s) 621, 1575, 1903, 2238, 3008
BstSFI CTRYAG 2 cut(s) 1172, 2776
BstSLI GKGCMC 1 cut(s) 1162
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 9 cut(s) 179, 226, 309, 381, 697, 2296, 2475, 2542, 2971
BstV2I GAAGAC 2 cut(s) 1095, 2839
BstX2I RGATCY 1 cut(s) 2998
BstXI CCANNNNNNTGG 2 cut(s) 203, 785
BstYI RGATCY 1 cut(s) 2998
BsuI GTATCC 1 cut(s) 2015
BtgI CCRYGG 1 cut(s) 1382
BtgZI GCGATG 1 cut(s) 2799
BtrI CACGTC 1 cut(s) 2900
BtsCI GGATG 7 cut(s) 208, 1106, 1372, 1776, 2533, 2889, 2953
BtsI GCAGTG 1 cut(s) 2042
BtsIMutI CAGTG 4 cut(s) 505, 951, 1019, 2042
BveI ACCTGC 2 cut(s) 1940, 2783
Cac8I GCNNGC 9 cut(s) 55, 244, 369, 450, 643, 647, 1796, 2126, 2426
CaiI CAGNNNCTG 4 cut(s) 698, 1085, 1870, 2965
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 174, 431, 860
Csp6I GTAC 3 cut(s) 234, 473, 2972
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviQI GTAC 3 cut(s) 234, 473, 2972
DpnI GATC 9 cut(s) 17, 105, 114, 318, 909, 942, 1501, 1703, 3000
DpnII GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
DrdI GACNNNNNNGTC 1 cut(s) 2705
DseDI GACNNNNNNGTC 1 cut(s) 2705
EaeI YGGCCR 1 cut(s) 715
Eam1104I CTCTTC 2 cut(s) 2415, 2900
EarI CTCTTC 2 cut(s) 2415, 2900
Ecl136II GAGCTC 2 cut(s) 517, 2387
Eco130I CCWWGG 4 cut(s) 23, 678, 1219, 1382
Eco147I AGGCCT 2 cut(s) 1580, 2128
Eco24I GRGCYC 2 cut(s) 519, 2389
Eco31I GGTCTC 3 cut(s) 1958, 2678, 3010
Eco47I GGWCC 2 cut(s) 174, 431
Eco53kI GAGCTC 2 cut(s) 517, 2387
Eco57I CTGAAG 4 cut(s) 1107, 1662, 2385, 2396
Eco88I CYCGRG 1 cut(s) 1582
EcoICRI GAGCTC 2 cut(s) 517, 2387
EcoNI CCTNNNNNAGG 1 cut(s) 1223
EcoRII CCWGG 5 cut(s) 621, 1575, 1903, 2238, 3008
EcoT14I CCWWGG 4 cut(s) 23, 678, 1219, 1382
EcoT38I GRGCYC 2 cut(s) 519, 2389
ErhI CCWWGG 4 cut(s) 23, 678, 1219, 1382
Esp3I CGTCTC 1 cut(s) 2890
FaqI GGGAC 2 cut(s) 1067, 1511
FauI CCCGC 1 cut(s) 1536
FblI GTMKAC 2 cut(s) 820, 2062
FokI GGATG 7 cut(s) 215, 1093, 1379, 1763, 2540, 2896, 2960
FriOI GRGCYC 2 cut(s) 519, 2389
FspBI CTAG 6 cut(s) 453, 747, 824, 1710, 2636, 2996
GlaI GCGC 1 cut(s) 299
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 4 cut(s) 593, 1461, 2730, 2987
HapII CCGG 1 cut(s) 570
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 821, 2766
HindII GTYRAC 2 cut(s) 821, 2766
HindIII AAGCTT 2 cut(s) 1888, 2473
HpaI GTTAAC 1 cut(s) 2766
HpaII CCGG 1 cut(s) 570
HphI GGTGA 7 cut(s) 244, 515, 559, 800, 1183, 1235, 2505
Hpy166II GTNNAC 9 cut(s) 791, 821, 1147, 1417, 2063, 2278, 2339, 2766, 2791
Hpy8I GTNNAC 9 cut(s) 791, 821, 1147, 1417, 2063, 2278, 2339, 2766, 2791
HpyAV CCTTC 8 cut(s) 515, 575, 879, 1019, 1605, 1791, 1853, 1889
HpyCH4III ACNGT 8 cut(s) 831, 1023, 1048, 1139, 1866, 2003, 2203, 2795
HpyCH4IV ACGT 3 cut(s) 1742, 2762, 2899
HpyF10VI GCNNNNNNNGC 8 cut(s) 328, 597, 723, 1896, 1946, 1966, 2552, 2660
HpySE526I ACGT 3 cut(s) 1742, 2762, 2899
HspAI GCGC 1 cut(s) 298
KspAI GTTAAC 1 cut(s) 2766
Kzo9I GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
LmnI GCTCC 5 cut(s) 682, 1668, 1680, 2079, 2347
Lsp1109I GCAGC 9 cut(s) 179, 226, 309, 381, 697, 2296, 2475, 2542, 2971
LweI GCATC 2 cut(s) 73, 2162
MaeI CTAG 6 cut(s) 453, 747, 824, 1710, 2636, 2996
MaeII ACGT 3 cut(s) 1742, 2762, 2899
MaeIII GTNAC 4 cut(s) 250, 503, 2014, 2707
MalI GATC 9 cut(s) 17, 105, 114, 318, 909, 942, 1501, 1703, 3000
MboI GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
MfeI CAATTG 4 cut(s) 326, 592, 614, 2055
MflI RGATCY 1 cut(s) 2998
MhlI GDGCHC 3 cut(s) 519, 1162, 2389
MlsI TGGCCA 1 cut(s) 717
MluNI TGGCCA 1 cut(s) 717
MlyI GAGTC 5 cut(s) 258, 286, 744, 1854, 2529
MmeI TCCRAC 5 cut(s) 1314, 1627, 1725, 1976, 2704
Mox20I TGGCCA 1 cut(s) 717
MroXI GAANNNNTTC 2 cut(s) 292, 919
MscI TGGCCA 1 cut(s) 717
MslI CAYNNNNRTG 4 cut(s) 1365, 1839, 2433, 2496
Msp20I TGGCCA 1 cut(s) 717
MspA1I CMGCKG 2 cut(s) 239, 322
MspI CCGG 1 cut(s) 570
MspR9I CCNGG 5 cut(s) 623, 1577, 1905, 2240, 3010
MunI CAATTG 4 cut(s) 326, 592, 614, 2055
Mva1269I GAATGC 2 cut(s) 1951, 2185
MvaI CCWGG 5 cut(s) 623, 1577, 1905, 2240, 3010
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 8 cut(s) 328, 597, 723, 1896, 1946, 1966, 2552, 2660
NcoI CCATGG 1 cut(s) 1382
NdeII GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
NlaIV GGNNCC 2 cut(s) 812, 1774
NmuCI GTSAC 3 cut(s) 250, 503, 2707
NspV TTCGAA 1 cut(s) 913
PaeR7I CTCGAG 1 cut(s) 1582
PceI AGGCCT 2 cut(s) 1580, 2128
PctI GAATGC 2 cut(s) 1951, 2185
PdmI GAANNNNTTC 2 cut(s) 292, 919
PfeI GAWTC 5 cut(s) 346, 656, 1231, 1357, 2927
PflMI CCANNNNNTGG 2 cut(s) 1388, 1672
PinAI ACCGGT 1 cut(s) 569
PleI GAGTC 5 cut(s) 257, 285, 744, 1853, 2529
PpsI GAGTC 5 cut(s) 257, 285, 744, 1853, 2529
PshBI ATTAAT 1 cut(s) 1623
Psp124BI GAGCTC 2 cut(s) 519, 2389
Psp1406I AACGTT 1 cut(s) 1742
Psp6I CCWGG 5 cut(s) 621, 1575, 1903, 2238, 3008
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 5 cut(s) 621, 1575, 1903, 2238, 3008
PspN4I GGNNCC 2 cut(s) 812, 1774
PspPI GGNCC 3 cut(s) 174, 431, 860
PspXI VCTCGAGB 1 cut(s) 1582
PstI CTGCAG 1 cut(s) 2780
PstNI CAGNNNCTG 4 cut(s) 698, 1085, 1870, 2965
PsuI RGATCY 1 cut(s) 2998
PvuII CAGCTG 2 cut(s) 239, 322
RsaI GTAC 3 cut(s) 235, 474, 2973
RsaNI GTAC 3 cut(s) 234, 473, 2972
RseI CAYNNNNRTG 4 cut(s) 1365, 1839, 2433, 2496
SacI GAGCTC 2 cut(s) 519, 2389
SalI GTCGAC 1 cut(s) 819
Sau3AI GATC 9 cut(s) 15, 103, 112, 316, 907, 940, 1499, 1701, 2998
Sau96I GGNCC 3 cut(s) 174, 431, 860
SbfI CCTGCAGG 1 cut(s) 2780
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 5 cut(s) 258, 286, 744, 1854, 2529
ScrFI CCNGG 5 cut(s) 623, 1577, 1905, 2240, 3010
SdaI CCTGCAGG 1 cut(s) 2780
SduI GDGCHC 3 cut(s) 519, 1162, 2389
SfaNI GCATC 2 cut(s) 73, 2162
SfcI CTRYAG 2 cut(s) 1172, 2776
Sfr274I CTCGAG 1 cut(s) 1582
SfuI TTCGAA 1 cut(s) 913
SinI GGWCC 2 cut(s) 174, 431
SlaI CTCGAG 1 cut(s) 1582
SmiMI CAYNNNNRTG 4 cut(s) 1365, 1839, 2433, 2496
Sse8387I CCTGCAGG 1 cut(s) 2780
SseBI AGGCCT 2 cut(s) 1580, 2128
SsiI CCGC 3 cut(s) 391, 1529, 2259
SspI AATATT 2 cut(s) 1923, 2599
SspMI CTAG 6 cut(s) 453, 747, 824, 1710, 2636, 2996
SstI GAGCTC 2 cut(s) 519, 2389
StuI AGGCCT 2 cut(s) 1580, 2128
StyD4I CCNGG 5 cut(s) 621, 1575, 1903, 2238, 3008
StyI CCWWGG 4 cut(s) 23, 678, 1219, 1382
TaaI ACNGT 8 cut(s) 831, 1023, 1048, 1139, 1866, 2003, 2203, 2795
TaiI ACGT 3 cut(s) 1745, 2765, 2902
TaqI TCGA 7 cut(s) 519, 820, 913, 1498, 1583, 2608, 3027
TatI WGTACW 1 cut(s) 472
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 5 cut(s) 346, 656, 1231, 1357, 2927
TscAI CASTG 4 cut(s) 505, 958, 1026, 2049
TseFI GTSAC 3 cut(s) 250, 503, 2707
TseI GCWGC 9 cut(s) 192, 239, 322, 394, 685, 2309, 2488, 2555, 2959
Tsp45I GTSAC 3 cut(s) 250, 503, 2707
TspDTI ATGAA 7 cut(s) 552, 624, 1113, 1773, 1872, 2508, 2919
TspGWI ACGGA 1 cut(s) 2577
TspRI CASTG 4 cut(s) 505, 958, 1026, 2049
Van91I CCANNNNNTGG 2 cut(s) 1388, 1672
VpaK11BI GGWCC 2 cut(s) 174, 431
VspI ATTAAT 1 cut(s) 1623
XagI CCTNNNNNAGG 1 cut(s) 1223
XapI RAATTY 7 cut(s) 847, 1015, 1213, 1282, 1302, 2354, 2645
XbaI TCTAGA 2 cut(s) 746, 2635
XhoI CTCGAG 1 cut(s) 1582
XmiI GTMKAC 2 cut(s) 820, 2062
XmnI GAANNNNTTC 2 cut(s) 292, 919
XspI CTAG 6 cut(s) 453, 747, 824, 1710, 2636, 2996
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.