MD11G1299000.v1.1

Protein TRANSPORT INHIBITOR RESPONSE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011947
Physical Location & Seq
Forward (+)
41561780 .. 41565545
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1299000.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGCCGAAAATGGCGAACTCGTTTCCCGAGGAGGTGCTGGAGCACGTGTTCTCATTCATACAGACGGACCAGGACCGGAACTCGATCTCGACGGTGTGCAAGTCGTGGTACGAGATCGAACGGTGGTGCAGGCGGCGGATTTTCATCGGGAACTGCTACGCCGTCAGCCCTAGGATGGTGATCAGGCGGTTCCCGGACATTAGATCCATCGAGCTCAAGGGAAAGCCTCACTTCGCTGACTTCAATCTGGTCCCCGAAGGCTGGGGTGGGTACGTGTACCCCTGGATCGCCGCCATGGCCAGCGCCTACCCTTGGCTCGAAGAGATCAAGCTCAAGAGGATGGTAGTCACCGACGAGACCTTGGAGCTGATTGCCAAGTCCTTCAAGAATTTCAAGCTCCTCGTGCTTTCGTCCTGTGAGGGGTTCAGTACTGATGGGCTCGCCGCCATTGCTGCCAATTGCAGGAATCTGCGGGAGCTGGACTTGCATGAGAGTGATGTGGAGGACCTAAGTGGGCATTGGCTCAGCCATTTTCCTGATACGTACACATCACTAGTTTCCCTCAACATTGCCTGCCTAGGGTCTGAATTGAGTTTCTCTGCCCTGGAGCGCCTGGTGGGTAGGTGTCCCAACCTGAGGAGTCTCCGGCTCAATCGTTCTGTGCCTCTTGACAGGCTAGCCAATCTACTTCGCCAGGCACCCAGGCTGAGTGAGTTAGGCACAGGGGCGTACTCGGCTGAGTTGCGGCCTGATATCTTCACAATCCTATCAGGTGCCTTCTCAAGATGCAAGGAACTCAAGAGCCTATCTGGATTTTGGGATGTGGTACCACCGTATCTTACGGCTATTTATTCTATCTGCCCTGGGCTAACATCACTAAATTTGAGTTATTCCAATATTCAAAGCCCGGATCTTGTAAAGCTTGTTAGCGAATGCCCGAATCTGCAGCGCCTCTGGGTATTGGATTACATTGAAGATGTTGGCCTTAATGCCCTGGCGGAATCTTGCAAGGATCTACGAGAGTTGAGGGTGTTCCCTTCAGATCCATTTGTGGTGGAACCAAATGTTTCCTTGACAGAGCAGGGCCTCATATCTGTCTCTGAGGGCTGTCCAAAGCTCCACTCAGTTCTGTACTTCTGCCGGCAAATGTCCAATGATGCATTAATCACCATTGCTAGGAATCAGCCGAACTTTACATGTTTTCGTCTTTGTATAATTGAGCCCAAAATTCCCGACTACCTGACCCTTCAGCCTCTTGATGTGGGTTTTGGGGCCATTGTTGAGAACTGCAAGAATCTCCGGCGATTGTCCATCTCTGGTCTACTAACTGATCGAGTGTTTGATTATATCGGAACCTATGGTAAAAATTTGGAGATGCTGTCTGTAGCCTTTGCCGGAGATAGTGACTTGGGGCTTCATCATATATTGTCTGGGTGCGATAATCTTCGTAAGCTGGAGATCAGGGACTGTCCTTTTGGTGACAAGGCTCTTTTGGCCAATGCTGCAAAGCTGGAGACAATGCGATCCCTTTGGATGTCTTCTTGCTCAGTGAGTTATGGAGCATGTAAGCTGCTAGGCCAGAAGTTGCCGAGGCTTAATGTGGAAGTCATTGATGAGAGGGGACACCCAGAATCCAGGCCGGAGAACTCTCCCGTTGAGAAGCTCTACATCTATAGGTCGGTTGCAGGGCCTAGGTTTGACATGCCTGGCTTTGTATGGACCATGGGTAAAGATTCGACGTTGAGGATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000822 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006464 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007584 GO:0008144 GO:0008150 GO:0008152 GO:0009267 GO:0009555 GO:0009605 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0009991 GO:0010011 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010152 GO:0010311 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0016567 GO:0016740 GO:0019005 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0036094 GO:0036211 GO:0038023 GO:0038198 GO:0042221 GO:0042562 GO:0042594 GO:0043167 GO:0043168 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045013 GO:0045014 GO:0045892 GO:0045934 GO:0045990 GO:0046015 GO:0048229 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060089 GO:0060255 GO:0061458 GO:0061984 GO:0061985 GO:0061986 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071496 GO:0071704 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:1905393 GO:1990234 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

585

Amino Acids

65.78

Weight (kDa)

6.21

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 7 - 45 9.9e-18 F-box
F-box-like PF12937 9 - 39 3.2e-06 F-box-like
Transp_inhibit PF18791 65 - 111 4.4e-26 Transport inhibitor response 1 protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62980
fragaria_vesca FvH4_2g24840
malus_domestica MD03G1276700.v1.1 MD11G1299000.v1.1
prunus_persica Prupe.8G253300_v2.0.a1
pyrus_communis pycom03g22040 pycom10g25620 pycom11g26270 pycom17g00400
rosa_chinensis RchiOBHm_Chr6g0292661
rosa_laevigata RLG00000011999
rosa_multiflora Rmu_sc0000515.1_g000020
rosa_roxburghii Rroxscaffold_7G00174780
rosa_rugosa Rorug06G0228900
rosa_samantha Rh6AG342200 Rh6BG349500 Rh6CG356400 Rh6DG342500
rosa_wichuraiana Rw6G029830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 826
AccB1I GGYRCC 3 cut(s) 697, 773, 826
AccI GTMKAC 1 cut(s) 1321
AciI CCGC 8 cut(s) 133, 136, 187, 291, 444, 472, 745, 998
AclWI GGATC 6 cut(s) 198, 293, 918, 1020, 1037, 1518
AcoI YGGCCR 2 cut(s) 297, 1494
AcsI RAATTY 4 cut(s) 388, 880, 1227, 1366
AcuI CTGAAG 2 cut(s) 1023, 1232
AcvI CACGTG 1 cut(s) 46
AfaI GTAC 8 cut(s) 110, 272, 278, 430, 545, 731, 828, 1133
AfiI CCNNNNNNNGG 8 cut(s) 175, 261, 312, 420, 462, 579, 636, 1176
AflIII ACRYGT 3 cut(s) 45, 273, 1196
AgsI TTSAA 5 cut(s) 244, 385, 394, 902, 974
AhlI ACTAGT 1 cut(s) 553
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
Alw21I GWGCWC 2 cut(s) 45, 216
Alw26I GTCTC 4 cut(s) 350, 647, 1102, 1508
AlwI GGATC 6 cut(s) 198, 293, 918, 1020, 1037, 1518
AlwNI CAGNNNCTG 1 cut(s) 1467
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 9 cut(s) 297, 746, 982, 1084, 1272, 1494, 1576, 1637, 1688
ApeKI GCWGC 4 cut(s) 452, 946, 1502, 1570
ApoI RAATTY 4 cut(s) 388, 880, 1227, 1366
ArsI GACNNNNNNTTYG 2 cut(s) 1457, 1489
AseI ATTAAT 1 cut(s) 1163
Asp718I GGTACC 1 cut(s) 826
AspA2I CCTAGG 3 cut(s) 170, 577, 1691
AspLEI GCGC 3 cut(s) 305, 612, 951
AspS9I GGNCC 8 cut(s) 67, 73, 250, 505, 1084, 1272, 1688, 1719
AsuC2I CCSGG 2 cut(s) 194, 908
AsuHPI GGTGA 4 cut(s) 190, 340, 1159, 1490
AsuNHI GCTAGC 1 cut(s) 676
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 5 cut(s) 67, 73, 250, 505, 1719
AvrII CCTAGG 3 cut(s) 170, 577, 1691
AxyI CCTNAGG 1 cut(s) 635
BaeI ACNNNNGTAYC 4 cut(s) 818, 818, 851, 851
BalI TGGCCA 2 cut(s) 299, 1496
BanI GGYRCC 3 cut(s) 697, 773, 826
BanII GRGCYC 3 cut(s) 216, 441, 1224
BauI CACGAG 1 cut(s) 401
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 1530
Bbv12I GWGCWC 2 cut(s) 45, 216
BbvI GCAGC 4 cut(s) 439, 958, 1489, 1557
BccI CCATC 5 cut(s) 169, 215, 334, 428, 1319
BceAI ACGGC 2 cut(s) 146, 858
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 2 cut(s) 194, 908
BcoDI GTCTC 4 cut(s) 350, 647, 1102, 1508
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 7 cut(s) 171, 554, 578, 677, 1176, 1574, 1692
BfmI CTRYAG 3 cut(s) 944, 1383, 1672
BfoI RGCGCY 3 cut(s) 306, 613, 952
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 8 cut(s) 134, 291, 444, 453, 746, 947, 1503, 1571
BlnI CCTAGG 3 cut(s) 170, 577, 1691
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 8 cut(s) 135, 292, 445, 454, 747, 948, 1504, 1572
BmcAI AGTACT 1 cut(s) 430
Bme18I GGWCC 5 cut(s) 67, 73, 250, 505, 1719
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 8 cut(s) 67, 73, 250, 505, 1084, 1272, 1688, 1719
BmiI GGNNCC 8 cut(s) 191, 252, 699, 775, 828, 1059, 1273, 1354
BmsI GCATC 3 cut(s) 776, 1147, 1365
BmtI GCTAGC 1 cut(s) 680
BpiI GAAGAC 1 cut(s) 1530
BpmI CTGGAG 4 cut(s) 59, 626, 1475, 1532
Bpu1102I GCTNAGC 1 cut(s) 524
BpuEI CTTGAG 4 cut(s) 200, 317, 766, 782
BpuMI CCSGG 2 cut(s) 194, 908
BsaAI YACGTR 3 cut(s) 46, 274, 543
BsaBI GATNNNNATC 2 cut(s) 143, 179
BsaI GGTCTC 1 cut(s) 350
BsaWI WCCGGW 1 cut(s) 75
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 1364, 1394
Bsc4I CCNNNNNNNGG 8 cut(s) 175, 261, 312, 420, 462, 579, 636, 1176
Bse118I RCCGGY 1 cut(s) 1140
Bse21I CCTNAGG 1 cut(s) 635
Bse3DI GCAATG 3 cut(s) 447, 567, 1170
Bse8I GATNNNNATC 2 cut(s) 143, 179
BseGI GGATG 4 cut(s) 180, 345, 826, 1539
BseJI GATNNNNATC 2 cut(s) 143, 179
BseLI CCNNNNNNNGG 8 cut(s) 175, 261, 312, 420, 462, 579, 636, 1176
BseMI GCAATG 3 cut(s) 447, 567, 1170
BseMII CTCAG 7 cut(s) 538, 626, 698, 729, 1092, 1137, 1560
BseRI GAGGAG 3 cut(s) 44, 389, 652
BseXI GCAGC 4 cut(s) 439, 958, 1489, 1557
BseYI CCCAGC 1 cut(s) 261
BsgI GTGCAG 1 cut(s) 148
BshFI GGCC 9 cut(s) 299, 748, 984, 1086, 1274, 1496, 1578, 1639, 1690
BshNI GGYRCC 3 cut(s) 697, 773, 826
BsiHKAI GWGCWC 2 cut(s) 45, 216
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 8 cut(s) 76, 194, 646, 908, 1141, 1300, 1395, 1640
BslFI GGGAC 4 cut(s) 236, 612, 1478, 1635
BslI CCNNNNNNNGG 8 cut(s) 175, 261, 312, 420, 462, 579, 636, 1176
BsmAI GTCTC 4 cut(s) 350, 647, 1102, 1508
BsmFI GGGAC 4 cut(s) 236, 612, 1478, 1635
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 9 cut(s) 299, 748, 984, 1086, 1274, 1496, 1578, 1639, 1690
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 4 cut(s) 45, 216, 441, 1224
Bsp1720I GCTNAGC 1 cut(s) 524
Bsp19I CCATGG 2 cut(s) 294, 1722
BspACI CCGC 8 cut(s) 133, 136, 187, 291, 444, 472, 745, 998
BspANI GGCC 9 cut(s) 299, 748, 984, 1086, 1274, 1496, 1578, 1639, 1690
BspCNI CTCAG 7 cut(s) 537, 627, 699, 730, 1093, 1136, 1559
BspLI GGNNCC 8 cut(s) 191, 252, 699, 775, 828, 1059, 1273, 1354
BspMAI CTGCAG 1 cut(s) 948
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 6 cut(s) 198, 293, 918, 1020, 1037, 1518
BspT107I GGYRCC 3 cut(s) 697, 773, 826
BspTNI GGTCTC 1 cut(s) 350
BsrDI GCAATG 3 cut(s) 447, 567, 1170
BsrFI RCCGGY 1 cut(s) 1140
BssAI RCCGGY 1 cut(s) 1140
BssSI CACGAG 1 cut(s) 401
BssT1I CCWWGG 7 cut(s) 170, 294, 311, 360, 577, 1691, 1722
Bst2BI CACGAG 1 cut(s) 401
Bst4CI ACNGT 4 cut(s) 94, 123, 834, 1469
Bst6I CTCTTC 1 cut(s) 315
BstBAI YACGTR 3 cut(s) 46, 274, 543
BstC8I GCNNGC 6 cut(s) 131, 301, 441, 574, 678, 1142
BstDEI CTNAG 8 cut(s) 509, 524, 635, 707, 738, 1101, 1123, 1546
BstDSI CCRYGG 2 cut(s) 294, 1722
BstF5I GGATG 4 cut(s) 180, 345, 826, 1539
BstH2I RGCGCY 3 cut(s) 306, 613, 952
BstHHI GCGC 3 cut(s) 305, 612, 951
BstMAI GTCTC 4 cut(s) 350, 647, 1102, 1508
BstMWI GCNNNNNNNGC 8 cut(s) 296, 403, 449, 452, 484, 734, 1493, 1502
BstNSI RCATGY 3 cut(s) 1200, 1566, 1705
BstSFI CTRYAG 3 cut(s) 944, 1383, 1672
BstSNI TACGTA 1 cut(s) 543
BstV1I GCAGC 4 cut(s) 439, 958, 1489, 1557
BstV2I GAAGAC 1 cut(s) 1530
BstX2I RGATCY 4 cut(s) 203, 910, 1012, 1042
BstYI RGATCY 4 cut(s) 203, 910, 1012, 1042
Bsu36I CCTNAGG 1 cut(s) 635
BsuRI GGCC 9 cut(s) 299, 748, 984, 1086, 1274, 1496, 1578, 1639, 1690
BtgI CCRYGG 2 cut(s) 294, 1722
BtsCI GGATG 4 cut(s) 180, 345, 826, 1539
BtsIMutI CAGTG 1 cut(s) 1554
Cac8I GCNNGC 6 cut(s) 131, 301, 441, 574, 678, 1142
CaiI CAGNNNCTG 1 cut(s) 1467
CfoI GCGC 3 cut(s) 305, 612, 951
Cfr10I RCCGGY 1 cut(s) 1140
Cfr13I GGNCC 8 cut(s) 67, 73, 250, 505, 1084, 1272, 1688, 1719
Csp6I GTAC 8 cut(s) 109, 271, 277, 429, 544, 730, 827, 1132
CviAII CATG 6 cut(s) 295, 488, 1197, 1563, 1702, 1723
CviQI GTAC 8 cut(s) 109, 271, 277, 429, 544, 730, 827, 1132
DdeI CTNAG 8 cut(s) 509, 524, 635, 707, 738, 1101, 1123, 1546
EaeI YGGCCR 2 cut(s) 297, 1494
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
EciI GGCGGA 2 cut(s) 151, 1013
Ecl136II GAGCTC 1 cut(s) 214
Eco105I TACGTA 1 cut(s) 543
Eco130I CCWWGG 7 cut(s) 170, 294, 311, 360, 577, 1691, 1722
Eco24I GRGCYC 3 cut(s) 216, 441, 1224
Eco31I GGTCTC 1 cut(s) 350
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 5 cut(s) 67, 73, 250, 505, 1719
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 1023, 1232
Eco72I CACGTG 1 cut(s) 46
Eco81I CCTNAGG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 3 cut(s) 505, 1084, 1688
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 7 cut(s) 170, 294, 311, 360, 577, 1691, 1722
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 3 cut(s) 216, 441, 1224
ErhI CCWWGG 7 cut(s) 170, 294, 311, 360, 577, 1691, 1722
FaeI CATG 6 cut(s) 298, 491, 1200, 1566, 1705, 1726
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 4 cut(s) 236, 612, 1478, 1635
FatI CATG 6 cut(s) 294, 487, 1196, 1562, 1701, 1722
FauI CCCGC 1 cut(s) 465
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 1321
Fnu4HI GCNGC 8 cut(s) 134, 291, 444, 453, 746, 947, 1503, 1571
FokI GGATG 4 cut(s) 187, 352, 833, 1546
FriOI GRGCYC 3 cut(s) 216, 441, 1224
Fsp4HI GCNGC 8 cut(s) 134, 291, 444, 453, 746, 947, 1503, 1571
FspBI CTAG 7 cut(s) 171, 554, 578, 677, 1176, 1574, 1692
GlaI GCGC 3 cut(s) 304, 611, 950
GluI GCNGC 8 cut(s) 134, 291, 444, 453, 746, 947, 1503, 1571
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 4 cut(s) 59, 626, 1475, 1532
HaeII RGCGCY 3 cut(s) 306, 613, 952
HaeIII GGCC 9 cut(s) 299, 748, 984, 1086, 1274, 1496, 1578, 1639, 1690
HapII CCGG 8 cut(s) 76, 194, 646, 908, 1141, 1300, 1395, 1640
HhaI GCGC 3 cut(s) 305, 612, 951
Hin1II CATG 6 cut(s) 298, 491, 1200, 1566, 1705, 1726
Hin6I GCGC 3 cut(s) 303, 610, 949
HinP1I GCGC 3 cut(s) 303, 610, 949
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 8 cut(s) 466, 640, 940, 1001, 1180, 1294, 1631, 1733
HpaII CCGG 8 cut(s) 76, 194, 646, 908, 1141, 1300, 1395, 1640
HphI GGTGA 4 cut(s) 190, 340, 1159, 1490
Hpy166II GTNNAC 3 cut(s) 277, 546, 1322
Hpy188I TCNGA 4 cut(s) 586, 1042, 1102, 1352
Hpy8I GTNNAC 3 cut(s) 277, 546, 1322
Hpy99I CGWCG 3 cut(s) 94, 356, 1741
HpyAV CCTTC 5 cut(s) 251, 391, 787, 1047, 1256
HpyCH4III ACNGT 4 cut(s) 94, 123, 834, 1469
HpyCH4IV ACGT 4 cut(s) 45, 273, 542, 1739
HpyF10VI GCNNNNNNNGC 8 cut(s) 296, 403, 449, 452, 484, 734, 1493, 1502
HpyF3I CTNAG 8 cut(s) 509, 524, 635, 707, 738, 1101, 1123, 1546
HpySE526I ACGT 4 cut(s) 45, 273, 542, 1739
Hsp92II CATG 6 cut(s) 298, 491, 1200, 1566, 1705, 1726
HspAI GCGC 3 cut(s) 303, 610, 949
KpnI GGTACC 1 cut(s) 830
KroI GCCGGC 1 cut(s) 1140
KroNI GCCGGC 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 180
LmnI GCTCC 7 cut(s) 40, 364, 402, 475, 607, 1122, 1559
Lsp1109I GCAGC 4 cut(s) 439, 958, 1489, 1557
LweI GCATC 3 cut(s) 776, 1147, 1365
MaeI CTAG 7 cut(s) 171, 554, 578, 677, 1176, 1574, 1692
MaeII ACGT 4 cut(s) 45, 273, 542, 1739
MaeIII GTNAC 3 cut(s) 346, 1403, 1478
MboII GAAGA 5 cut(s) 332, 748, 986, 1436, 1530
MfeI CAATTG 1 cut(s) 457
MflI RGATCY 4 cut(s) 203, 910, 1012, 1042
MhlI GDGCHC 4 cut(s) 45, 216, 441, 1224
MlsI TGGCCA 2 cut(s) 299, 1496
MluCI AATT 7 cut(s) 388, 457, 587, 880, 1215, 1227, 1366
MluNI TGGCCA 2 cut(s) 299, 1496
MlyI GAGTC 1 cut(s) 649
Mox20I TGGCCA 2 cut(s) 299, 1496
Mph1103I ATGCAT 1 cut(s) 1162
MroNI GCCGGC 1 cut(s) 1140
MscI TGGCCA 2 cut(s) 299, 1496
MseI TTAA 3 cut(s) 987, 1163, 1596
MslI CAYNNNNRTG 1 cut(s) 492
Msp20I TGGCCA 2 cut(s) 299, 1496
MspI CCGG 8 cut(s) 76, 194, 646, 908, 1141, 1300, 1395, 1640
MunI CAATTG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 938
MwoI GCNNNNNNNGC 8 cut(s) 296, 403, 449, 452, 484, 734, 1493, 1502
NaeI GCCGGC 1 cut(s) 1142
NciI CCSGG 2 cut(s) 194, 908
NcoI CCATGG 2 cut(s) 294, 1722
NgoMIV GCCGGC 1 cut(s) 1140
NheI GCTAGC 1 cut(s) 676
NlaIII CATG 6 cut(s) 298, 491, 1200, 1566, 1705, 1726
NlaIV GGNNCC 8 cut(s) 191, 252, 699, 775, 828, 1059, 1273, 1354
NmeAIII GCCGAG 2 cut(s) 713, 1614
NmuCI GTSAC 3 cut(s) 346, 1403, 1478
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 3 cut(s) 1200, 1566, 1705
PasI CCCWGGG 1 cut(s) 863
PciI ACATGT 1 cut(s) 1196
PctI GAATGC 1 cut(s) 938
PdiI GCCGGC 1 cut(s) 1142
PfeI GAWTC 7 cut(s) 466, 940, 1001, 1180, 1294, 1631, 1733
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 8 cut(s) 135, 292, 445, 454, 747, 948, 1504, 1572
PleI GAGTC 1 cut(s) 648
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
PpsI GAGTC 1 cut(s) 648
Ppu21I YACGTR 3 cut(s) 46, 274, 543
PpuMI RGGWCCY 1 cut(s) 505
PscI ACATGT 1 cut(s) 1196
PshBI ATTAAT 1 cut(s) 1163
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 505
PspCI CACGTG 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 261
PspN4I GGNNCC 8 cut(s) 191, 252, 699, 775, 828, 1059, 1273, 1354
PspPI GGNCC 8 cut(s) 67, 73, 250, 505, 1084, 1272, 1688, 1719
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 948
PstNI CAGNNNCTG 1 cut(s) 1467
PsuI RGATCY 4 cut(s) 203, 910, 1012, 1042
RsaI GTAC 8 cut(s) 110, 272, 278, 430, 545, 731, 828, 1133
RsaNI GTAC 8 cut(s) 109, 271, 277, 429, 544, 730, 827, 1132
RseI CAYNNNNRTG 1 cut(s) 492
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 3 cut(s) 987, 1163, 1596
SatI GCNGC 8 cut(s) 134, 291, 444, 453, 746, 947, 1503, 1571
Sau96I GGNCC 8 cut(s) 67, 73, 250, 505, 1084, 1272, 1688, 1719
ScaI AGTACT 1 cut(s) 430
SchI GAGTC 1 cut(s) 649
SduI GDGCHC 4 cut(s) 45, 216, 441, 1224
SfaNI GCATC 3 cut(s) 776, 1147, 1365
SfcI CTRYAG 3 cut(s) 944, 1383, 1672
SinI GGWCC 5 cut(s) 67, 73, 250, 505, 1719
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 4 cut(s) 215, 332, 781, 797
SmoI CTYRAG 4 cut(s) 215, 332, 781, 797
SnaBI TACGTA 1 cut(s) 543
SpeI ACTAGT 1 cut(s) 553
Sse9I AATT 7 cut(s) 388, 457, 587, 880, 1215, 1227, 1366
SsiI CCGC 8 cut(s) 133, 136, 187, 291, 444, 472, 745, 998
SspI AATATT 1 cut(s) 898
SspMI CTAG 7 cut(s) 171, 554, 578, 677, 1176, 1574, 1692
SstI GAGCTC 1 cut(s) 216
StyI CCWWGG 7 cut(s) 170, 294, 311, 360, 577, 1691, 1722
TaaI ACNGT 4 cut(s) 94, 123, 834, 1469
TaiI ACGT 4 cut(s) 48, 276, 545, 1742
TaqI TCGA 7 cut(s) 83, 89, 117, 210, 318, 1333, 1736
TasI AATT 7 cut(s) 388, 457, 587, 880, 1215, 1227, 1366
TatI WGTACW 2 cut(s) 428, 1131
TauI GCSGC 4 cut(s) 136, 293, 446, 748
TfiI GAWTC 7 cut(s) 466, 940, 1001, 1180, 1294, 1631, 1733
Tru1I TTAA 3 cut(s) 987, 1163, 1596
Tru9I TTAA 3 cut(s) 987, 1163, 1596
TscAI CASTG 1 cut(s) 1554
TseFI GTSAC 3 cut(s) 346, 1403, 1478
TseI GCWGC 4 cut(s) 452, 946, 1502, 1570
Tsp45I GTSAC 3 cut(s) 346, 1403, 1478
TspDTI ATGAA 3 cut(s) 46, 133, 1406
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 1 cut(s) 1554
VpaK11BI GGWCC 5 cut(s) 67, 73, 250, 505, 1719
VspI ATTAAT 1 cut(s) 1163
XapI RAATTY 4 cut(s) 388, 880, 1227, 1366
XceI RCATGY 3 cut(s) 1200, 1566, 1705
XmaJI CCTAGG 3 cut(s) 170, 577, 1691
XmiI GTMKAC 1 cut(s) 1321
XspI CTAG 7 cut(s) 171, 554, 578, 677, 1176, 1574, 1692
ZrmI AGTACT 1 cut(s) 430
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.