MD12G1003900.v1.1

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008975
Physical Location & Seq
Forward (+)
555377 .. 558470
3094 bp
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UTR
Exon/CDS
Intron
MD12G1003900.v1.1.491

Sequence Viewer

Length: 615 bp
ATGTCTACTTTGAACAATTCAACAAATATATTTTGGCAAGAATGTCAGGTTGGGAAGGTTGATAGGCAGAAACTACTCAATCAAAAGGGATGTGTTGTATGGATTACTGGTCTCAGTGGATCAGGGAAAAGTACACTGGCATACTCATTAAGTAGAGAACTATACTCTAGGGGAAAGTTGTCATATGTCCTTGATGGAGATAACCTTCGGCATGGTCTGAACAAGGATCTTGGTTTCAAAGCCGAAGACCGAGCTGAAAATATACGCAGAGTTGGGGAGGTAGCAAAACTGTTTGCAGATGCAGGATTGTTTAGTATTGCTAGCCTCATATCTCCTTATAAGAAAGATCGGGATGCCTGCCGTGCATTGTTACCTGATGCAAACTTTATTGAGGCAAGACTCCATATGGTTCTTTCAAGAGATGCAAAAGGACTTTATAAGCTTGCACGTGCTGGAAAGATTAAAGGTTTTACTGGAATAGATGACCCTTATGAGCGACCAGAGAACTGCGAGATAGAAATAGGACAAGAAAATGGAGTTTGTCCCATGCCTGCAGCCATGGCAGGCCGAGTAGTGTCCTACTTGGAGGAGAAAGGATTTCTTCAAGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00920 ko01100 ko01120 map00230 map00920 map01100 map01120
Pfam Domains
Protein Families
APS_kinase

Protein Analysis

205

Amino Acids

22.61

Weight (kDa)

8.76

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 29 - 174 8.5e-61 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 339, 438
AccI GTMKAC 1 cut(s) 5
AclWI GGATC 2 cut(s) 127, 234
AcvI CACGTG 1 cut(s) 449
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 5 cut(s) 13, 21, 238, 417, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AluBI AGCT 2 cut(s) 254, 442
AluI AGCT 2 cut(s) 254, 442
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 2 cut(s) 127, 234
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 554
AsuNHI GCTAGC 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 566
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 168, 321
BfmI CTRYAG 1 cut(s) 552
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
BmsI GCATC 4 cut(s) 289, 343, 367, 412
BmtI GCTAGC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 449
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 558
Bse1I ACTGG 3 cut(s) 112, 141, 478
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 2 cut(s) 95, 358
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 3 cut(s) 112, 141, 478
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 1 cut(s) 566
BshFI GGCC 1 cut(s) 567
BslFI GGGAC 1 cut(s) 528
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 528
BsnI GGCC 1 cut(s) 567
Bso31I GGTCTC 1 cut(s) 116
Bsp143I GATC 3 cut(s) 119, 226, 346
Bsp19I CCATGG 1 cut(s) 558
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 556
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 127, 234
BspTNI GGTCTC 1 cut(s) 116
BsrI ACTGG 3 cut(s) 112, 141, 478
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 3 cut(s) 119, 226, 346
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 5 cut(s) 322, 358, 444, 552, 565
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 2 cut(s) 95, 358
BstKTI GATC 3 cut(s) 122, 229, 349
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 3 cut(s) 119, 226, 346
BstMWI GCNNNNNNNGC 2 cut(s) 362, 560
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 566
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 567
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 2 cut(s) 95, 358
BtsIMutI CAGTG 2 cut(s) 121, 134
Cac8I GCNNGC 5 cut(s) 322, 358, 444, 552, 565
Csp6I GTAC 1 cut(s) 132
CviAII CATG 3 cut(s) 212, 547, 559
CviJI RGCY 6 cut(s) 242, 254, 324, 442, 557, 567
CviKI_1 RGCY 6 cut(s) 242, 254, 324, 442, 557, 567
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 113
DpnI GATC 3 cut(s) 121, 228, 348
DpnII GATC 3 cut(s) 119, 226, 346
Eco130I CCWWGG 1 cut(s) 558
Eco31I GGTCTC 1 cut(s) 116
Eco72I CACGTG 1 cut(s) 449
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 3 cut(s) 215, 550, 562
FalI AAGNNNNNCTT 1 cut(s) 585
FaqI GGGAC 1 cut(s) 528
FatI CATG 3 cut(s) 211, 546, 558
FauNDI CATATG 2 cut(s) 184, 405
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 555
FokI GGATG 2 cut(s) 102, 365
Fsp4HI GCNGC 1 cut(s) 555
FspBI CTAG 2 cut(s) 168, 321
GluI GCNGC 1 cut(s) 555
HaeIII GGCC 1 cut(s) 567
Hin1II CATG 3 cut(s) 215, 550, 562
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 1 cut(s) 399
Hpy166II GTNNAC 2 cut(s) 6, 134
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 2 cut(s) 350, 417
Hpy8I GTNNAC 2 cut(s) 6, 134
HpyAV CCTTC 2 cut(s) 49, 215
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 1 cut(s) 448
HpyCH4V TGCA 7 cut(s) 296, 302, 365, 380, 425, 446, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 560
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 448
Hsp92II CATG 3 cut(s) 215, 550, 562
Kzo9I GATC 3 cut(s) 119, 226, 346
Lsp1109I GCAGC 1 cut(s) 566
LweI GCATC 4 cut(s) 289, 343, 367, 412
MaeI CTAG 2 cut(s) 168, 321
MaeII ACGT 1 cut(s) 448
MaeIII GTNAC 1 cut(s) 369
MalI GATC 3 cut(s) 121, 228, 348
MboI GATC 3 cut(s) 119, 226, 346
MboII GAAGA 2 cut(s) 257, 593
MflI RGATCY 1 cut(s) 226
MluCI AATT 1 cut(s) 16
MlyI GAGTC 1 cut(s) 393
MnlI CCTC 4 cut(s) 271, 335, 385, 580
MseI TTAA 2 cut(s) 149, 462
MwoI GCNNNNNNNGC 2 cut(s) 362, 560
NcoI CCATGG 1 cut(s) 558
NdeI CATATG 2 cut(s) 184, 405
NdeII GATC 3 cut(s) 119, 226, 346
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 3 cut(s) 215, 550, 562
NmeAIII GCCGAG 1 cut(s) 593
PkrI GCNGC 1 cut(s) 556
PleI GAGTC 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 1 cut(s) 393
Ppu21I YACGTR 1 cut(s) 449
PsiI TTATAA 2 cut(s) 339, 438
PspCI CACGTG 1 cut(s) 449
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 149, 462
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 3 cut(s) 119, 226, 346
SchI GAGTC 1 cut(s) 393
SetI ASST 9 cut(s) 51, 60, 207, 256, 282, 376, 444, 451, 469
SfaNI GCATC 4 cut(s) 289, 343, 367, 412
SfcI CTRYAG 1 cut(s) 552
Sse9I AATT 1 cut(s) 16
SspMI CTAG 2 cut(s) 168, 321
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 1 cut(s) 451
TaqII GACCGA 1 cut(s) 264
TasI AATT 1 cut(s) 16
TatI WGTACW 1 cut(s) 131
Tru1I TTAA 2 cut(s) 149, 462
Tru9I TTAA 2 cut(s) 149, 462
TscAI CASTG 2 cut(s) 121, 141
TseI GCWGC 1 cut(s) 554
TspRI CASTG 2 cut(s) 121, 141
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 168, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.