Rh3BG370800

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008975
Physical Location & Seq
Forward (+)
46751330 .. 46756384
5055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG370800.1

Sequence Viewer

Length: 561 bp
ATGTCTACTATCAGCAACTCAACAAATATATTTTGGCAAGAATGTCCTGTTGGGAAAGCTGAAAGGCAGAAACTACTCAACCAAAAGGGCTGTGTTGTATGGATTACAGGTCTCAGCGGATCAGGGAAAAGTACACTGGCGTGCTCACTAAGTAGAGAACTGCACTCGAGGGGAAAGCTGTCATATATCCTTGATGGTGATAACCTTCGACATGGTATCAACAAGGATCTTGGTTTCAAACCAGAAGACCGGACTGAAAATATTCGCAGAGTTGGGGAGATCGCAAAACTGTTTGCGGATGCAGGATTGATCTGTATTGCTAGTCTGATATCTCCTTATAGGAAAGATCGTGATGCTTGCCGTGCAATGCTACCAGATGCAAATTTTATTGAGGTTTTCATGAACATGCCCCTAGAGTTATGTGAGTCAAGGGATGCAAAAGGCCTTTACAAGCTTGCACGTGCGGGAAAGATTAGAGGTGTGTTTTTGAAACATATTTCAACTATCCTACATTTCCAACTGTTCCTCCATCGCTTCAGAACAGAAAGAGATTTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00920 ko01100 ko01120 map00230 map00920 map01100 map01120
Pfam Domains
Protein Families
APS_kinase

Protein Analysis

186

Amino Acids

21.03

Weight (kDa)

9.45

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 29 - 160 2.3e-58 Adenylylsulphate kinase
AAA_33 PF13671 32 - 147 3.8e-08 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 117, 296, 464
AclWI GGATC 2 cut(s) 127, 234
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 520
AcvI CACGTG 1 cut(s) 461
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 3 cut(s) 238, 490, 501
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AleI CACNNNNGTG 1 cut(s) 139
AluBI AGCT 3 cut(s) 59, 178, 454
AluI AGCT 3 cut(s) 59, 178, 454
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 2 cut(s) 127, 234
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 382
Asp700I GAANNNNTTC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 166
BbrPI CACGTG 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 146
BccI CCATC 2 cut(s) 188, 537
BceAI ACGGC 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 321, 413
BmeT110I CYCGRG 1 cut(s) 166
BmsI GCATC 4 cut(s) 289, 343, 367, 424
BpiI GAAGAC 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 116
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bse1I ACTGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 372
BseGI GGATG 2 cut(s) 304, 439
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 1 cut(s) 444
BsiHKAI GWGCWC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 116
BsnI GGCC 1 cut(s) 444
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 5 cut(s) 119, 226, 279, 309, 346
BspACI CCGC 3 cut(s) 117, 296, 464
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 399
BspPI GGATC 2 cut(s) 127, 234
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 141
BssMI GATC 5 cut(s) 119, 226, 279, 309, 346
Bst4CI ACNGT 2 cut(s) 291, 522
BstBAI YACGTR 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 142, 358, 456
BstDEI CTNAG 2 cut(s) 113, 149
BstF5I GGATG 2 cut(s) 304, 439
BstKTI GATC 5 cut(s) 122, 229, 282, 312, 349
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 5 cut(s) 119, 226, 279, 309, 346
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 2 cut(s) 304, 439
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 142, 358, 456
CciI TCATGA 1 cut(s) 399
Csp6I GTAC 1 cut(s) 132
CviAII CATG 3 cut(s) 212, 400, 406
CviJI RGCY 5 cut(s) 59, 90, 178, 444, 454
CviKI_1 RGCY 5 cut(s) 59, 90, 178, 444, 454
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 2 cut(s) 113, 149
DpnI GATC 5 cut(s) 121, 228, 281, 311, 348
DpnII GATC 5 cut(s) 119, 226, 279, 309, 346
DraI TTTAAA 1 cut(s) 555
Eco147I AGGCCT 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 520
Eco72I CACGTG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 330
FaeI CATG 3 cut(s) 215, 403, 409
FatI CATG 3 cut(s) 211, 399, 405
FauI CCCGC 1 cut(s) 457
FblI GTMKAC 1 cut(s) 5
FokI GGATG 2 cut(s) 311, 446
FspBI CTAG 2 cut(s) 321, 413
HaeIII GGCC 1 cut(s) 444
HapII CCGG 1 cut(s) 250
Hin1II CATG 3 cut(s) 215, 403, 409
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 1 cut(s) 425
HpaII CCGG 1 cut(s) 250
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 2 cut(s) 6, 134
Hpy188I TCNGA 2 cut(s) 327, 539
Hpy188III TCNNGA 2 cut(s) 350, 400
Hpy8I GTNNAC 2 cut(s) 6, 134
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 2 cut(s) 291, 522
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 6 cut(s) 163, 302, 365, 380, 437, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 2 cut(s) 113, 149
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 3 cut(s) 215, 403, 409
Kzo9I GATC 5 cut(s) 119, 226, 279, 309, 346
LpnPI CCDG 8 cut(s) 60, 93, 108, 122, 255, 263, 288, 387
LweI GCATC 4 cut(s) 289, 343, 367, 424
MaeI CTAG 2 cut(s) 321, 413
MaeII ACGT 1 cut(s) 460
MalI GATC 5 cut(s) 121, 228, 281, 311, 348
MboI GATC 5 cut(s) 119, 226, 279, 309, 346
MboII GAAGA 1 cut(s) 257
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 2 cut(s) 382, 556
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 4 cut(s) 162, 385, 470, 536
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 2 cut(s) 139, 404
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 5 cut(s) 119, 226, 279, 309, 346
NlaIII CATG 3 cut(s) 215, 403, 409
NspI RCATGY 1 cut(s) 409
OliI CACNNNNGTG 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 166
PagI TCATGA 1 cut(s) 399
PceI AGGCCT 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 261
PleI GAGTC 1 cut(s) 433
PmaCI CACGTG 1 cut(s) 461
PmlI CACGTG 1 cut(s) 461
PpsI GAGTC 1 cut(s) 433
Ppu21I YACGTR 1 cut(s) 461
PspCI CACGTG 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 166
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 2 cut(s) 139, 404
SaqAI TTAA 1 cut(s) 554
Sau3AI GATC 5 cut(s) 119, 226, 279, 309, 346
SchI GAGTC 1 cut(s) 434
SduI GDGCHC 1 cut(s) 146
SetI ASST 8 cut(s) 61, 112, 180, 207, 396, 456, 463, 481
SfaNI GCATC 4 cut(s) 289, 343, 367, 424
Sfr274I CTCGAG 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 166
SmiI ATTTAAAT 1 cut(s) 555
SmiMI CAYNNNNRTG 2 cut(s) 139, 404
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 2 cut(s) 382, 556
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 3 cut(s) 117, 296, 464
SspI AATATT 1 cut(s) 262
SspMI CTAG 2 cut(s) 321, 413
StuI AGGCCT 1 cut(s) 444
SwaI ATTTAAAT 1 cut(s) 555
TaaI ACNGT 2 cut(s) 291, 522
TaiI ACGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 167, 208
TasI AATT 2 cut(s) 382, 556
TatI WGTACW 1 cut(s) 131
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 1 cut(s) 141
TspDTI ATGAA 2 cut(s) 388, 416
TspRI CASTG 1 cut(s) 141
XapI RAATTY 1 cut(s) 382
XceI RCATGY 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 2 cut(s) 321, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.