Rroxscaffold_6G00388430

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008975
Physical Location & Seq
Reverse (-)
1376171 .. 1380334
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388430.1

Sequence Viewer

Length: 768 bp
ATGTCTACTATCAGCAACTCAACAAATATATTTTGGCAAGAATGTCCTGTTGGGAAAGCTGAAAGGCAGAAACTACTCAACCAAAAGGGCTGTGTTGTATGGATTACAGGTCTCAGCGGATCAGTGTTTAGAGTGAAGAATTCCGATAAAGATAACGACACATCAGACATTTTATCATGTGATATAGAGAAACTTGAGGACATGGATGTCAGTTTCCAAGGGAAAAGTACACTGGCGTGCTCACTAAGTAGAGAACTGCACTCGAGGGGAAAGCTGTCATATATCCTTGATGGTGATAACCTTCGACATGGTATCAACAAGGATCTTGGTTTCAAACCAGAAGACCGGACTGAAAATATTCGCAGAGTTGGGGAGATTGCAAAACTGTTTGCGGATGCAGGATTGATATGTATTGCTAGTCTGATATCTCCTTATAGGAAAGATCGTGATGCTTGCCGTGCAATGCTACCAGATGCAAATTTTATTGAGGCAAGATGCAAGACTCCTTTTTATGGCTTTTATTGTTATAATCTGGTTTTCATGAACATGCCCCTAGAGTTATGTGAGTCAAGGGATGCAAAAGGCCTTTACAAGCTTGCACGTGCGGGAAAGATTAGAGGTTTTACCGGAATTGATGATCCATATGAACAACCACTGAACTGTGAGATAGAAATACAGCAAAATAATGAAGTTTGTCCCCTGCCTGCAGCCCTGGCAGGCCAGGTAGTGACATACTTGGAGGAGAAAGGCTTTCTTCAAGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00920 ko01100 ko01120 map00230 map00920 map01100 map01120
Pfam Domains
Protein Families
APS_kinase

Protein Analysis

255

Amino Acids

28.63

Weight (kDa)

5.45

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 71 - 225 3.5e-54 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
AasI GACNNNNNNGTC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 117, 392, 605
AclWI GGATC 3 cut(s) 127, 330, 632
AcsI RAATTY 2 cut(s) 139, 478
AcvI CACGTG 1 cut(s) 602
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 2 cut(s) 334, 758
AjnI CCWGG 2 cut(s) 711, 720
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AleI CACNNNNGTG 1 cut(s) 235
AluBI AGCT 3 cut(s) 59, 274, 595
AluI AGCT 3 cut(s) 59, 274, 595
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 3 cut(s) 127, 330, 632
Ama87I CYCGRG 1 cut(s) 262
AoxI GGCC 2 cut(s) 583, 718
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 2 cut(s) 139, 478
Asp700I GAANNNNTTC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 262
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 719
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 2 cut(s) 713, 722
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 417, 554
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 1 cut(s) 708
BlsI GCNGC 1 cut(s) 709
Bme1390I CCNGG 2 cut(s) 713, 722
BmeT110I CYCGRG 1 cut(s) 262
BmrFI CCNGG 2 cut(s) 713, 722
BmsI GCATC 5 cut(s) 385, 439, 463, 485, 565
BpiI GAAGAC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 215
BsaAI YACGTR 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 217, 711
BsaWI WCCGGW 2 cut(s) 345, 626
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 2 cut(s) 713, 722
BseDI CCNNGG 2 cut(s) 217, 711
BseGI GGATG 3 cut(s) 211, 400, 580
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 1 cut(s) 719
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 2 cut(s) 585, 720
BsiHKAI GWGCWC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 262
BsiSI CCGG 2 cut(s) 346, 627
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 681
BsnI GGCC 2 cut(s) 585, 720
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 4 cut(s) 119, 322, 442, 637
BspACI CCGC 3 cut(s) 117, 392, 605
BspANI GGCC 2 cut(s) 585, 720
BspCNI CTCAG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 540
BspMAI CTGCAG 1 cut(s) 709
BspPI GGATC 3 cut(s) 127, 330, 632
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 217, 711
BssMI GATC 4 cut(s) 119, 322, 442, 637
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 713, 722
Bst4CI ACNGT 2 cut(s) 387, 662
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 5 cut(s) 238, 454, 597, 705, 718
BstDEI CTNAG 2 cut(s) 113, 245
BstF5I GGATG 3 cut(s) 211, 400, 580
BstKTI GATC 4 cut(s) 122, 325, 445, 640
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 4 cut(s) 119, 322, 442, 637
BstMWI GCNNNNNNNGC 2 cut(s) 458, 713
BstNI CCWGG 2 cut(s) 713, 722
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 2 cut(s) 711, 720
BstSFI CTRYAG 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 719
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 2 cut(s) 585, 720
BtsCI GGATG 3 cut(s) 211, 400, 580
BtsIMutI CAGTG 3 cut(s) 129, 230, 653
Cac8I GCNNGC 5 cut(s) 238, 454, 597, 705, 718
CciI TCATGA 1 cut(s) 540
Csp6I GTAC 1 cut(s) 228
CviAII CATG 5 cut(s) 177, 202, 308, 541, 547
CviJI RGCY 9 cut(s) 59, 90, 274, 516, 585, 595, 710, 720, 750
CviKI_1 RGCY 9 cut(s) 59, 90, 274, 516, 585, 595, 710, 720, 750
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 2 cut(s) 113, 245
DpnI GATC 4 cut(s) 121, 324, 444, 639
DpnII GATC 4 cut(s) 119, 322, 442, 637
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 426
Eco72I CACGTG 1 cut(s) 602
Eco88I CYCGRG 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 711, 720
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 5 cut(s) 180, 205, 311, 544, 550
FalI AAGNNNNNCTT 1 cut(s) 738
FaqI GGGAC 1 cut(s) 681
FatI CATG 5 cut(s) 176, 201, 307, 540, 546
FauI CCCGC 1 cut(s) 598
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 708
FokI GGATG 3 cut(s) 218, 407, 587
Fsp4HI GCNGC 1 cut(s) 708
FspBI CTAG 2 cut(s) 417, 554
GluI GCNGC 1 cut(s) 708
HaeIII GGCC 2 cut(s) 585, 720
HapII CCGG 2 cut(s) 346, 627
Hin1II CATG 5 cut(s) 180, 205, 311, 544, 550
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 2 cut(s) 502, 566
HpaII CCGG 2 cut(s) 346, 627
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 2 cut(s) 6, 230
Hpy188I TCNGA 3 cut(s) 145, 166, 423
Hpy188III TCNNGA 2 cut(s) 446, 541
Hpy8I GTNNAC 2 cut(s) 6, 230
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 2 cut(s) 387, 662
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 9 cut(s) 259, 380, 398, 461, 476, 498, 578, 599, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 713
HpyF3I CTNAG 2 cut(s) 113, 245
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 5 cut(s) 180, 205, 311, 544, 550
Kzo9I GATC 4 cut(s) 119, 322, 442, 637
Lsp1109I GCAGC 1 cut(s) 719
LweI GCATC 5 cut(s) 385, 439, 463, 485, 565
MaeI CTAG 2 cut(s) 417, 554
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 727
MalI GATC 4 cut(s) 121, 324, 444, 639
MboI GATC 4 cut(s) 119, 322, 442, 637
MboII GAAGA 3 cut(s) 148, 353, 746
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 3 cut(s) 139, 478, 630
MlyI GAGTC 2 cut(s) 496, 575
MnlI CCTC 5 cut(s) 190, 258, 481, 611, 733
MroXI GAANNNNTTC 1 cut(s) 357
MslI CAYNNNNRTG 2 cut(s) 235, 545
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 2 cut(s) 346, 627
MspR9I CCNGG 2 cut(s) 713, 722
MvaI CCWGG 2 cut(s) 713, 722
MwoI GCNNNNNNNGC 2 cut(s) 458, 713
NdeI CATATG 1 cut(s) 643
NdeII GATC 4 cut(s) 119, 322, 442, 637
NlaIII CATG 5 cut(s) 180, 205, 311, 544, 550
NmuCI GTSAC 1 cut(s) 727
NspI RCATGY 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 262
PagI TCATGA 1 cut(s) 540
PceI AGGCCT 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 357
PkrI GCNGC 1 cut(s) 709
PleI GAGTC 2 cut(s) 496, 574
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 2 cut(s) 496, 574
Ppu21I YACGTR 1 cut(s) 602
PsiI TTATAA 1 cut(s) 528
Psp6I CCWGG 2 cut(s) 711, 720
PspCI CACGTG 1 cut(s) 602
PspGI CCWGG 2 cut(s) 711, 720
PspXI VCTCGAGB 1 cut(s) 262
PstI CTGCAG 1 cut(s) 709
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 2 cut(s) 235, 545
SatI GCNGC 1 cut(s) 708
Sau3AI GATC 4 cut(s) 119, 322, 442, 637
SchI GAGTC 2 cut(s) 496, 575
ScrFI CCNGG 2 cut(s) 713, 722
SduI GDGCHC 1 cut(s) 242
SetI ASST 8 cut(s) 61, 112, 276, 303, 597, 604, 622, 726
SfaNI GCATC 5 cut(s) 385, 439, 463, 485, 565
SfcI CTRYAG 1 cut(s) 705
Sfr274I CTCGAG 1 cut(s) 262
SlaI CTCGAG 1 cut(s) 262
SmiMI CAYNNNNRTG 2 cut(s) 235, 545
SmlI CTYRAG 2 cut(s) 194, 262
SmoI CTYRAG 2 cut(s) 194, 262
Sse9I AATT 3 cut(s) 139, 478, 630
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 3 cut(s) 117, 392, 605
SspI AATATT 1 cut(s) 358
SspMI CTAG 2 cut(s) 417, 554
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 711, 720
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 387, 662
TaiI ACGT 1 cut(s) 604
TaqI TCGA 2 cut(s) 263, 304
TasI AATT 3 cut(s) 139, 478, 630
TatI WGTACW 1 cut(s) 227
TscAI CASTG 3 cut(s) 129, 237, 660
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 1 cut(s) 707
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 4 cut(s) 529, 557, 660, 702
TspRI CASTG 3 cut(s) 129, 237, 660
XapI RAATTY 2 cut(s) 139, 478
XceI RCATGY 1 cut(s) 550
XhoI CTCGAG 1 cut(s) 262
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 417, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.