RLG00000022484

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008975
Physical Location & Seq
Reverse (-)
1442863 .. 1447940
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022484

Sequence Viewer

Length: 882 bp
ATGGGTGAGAGATTGACGCGCTTACATCTTGGTGTAGATTTTTGCAGCACCAACTGGGCATGCCTTTCCACACCTCAAGCTACGCAATGGAAGGAAGAGGAGGATGCGTCCAGATTCATTGACAGCCCCAAAAAGAAGCAAAAGACCCCCTTGAAAGCAGGGAATACCAATAAATGGAAAAACTTCTTATTCTTCGTCGCTTCCATCCCTCCCGGCCTCGAATTAAGTGTCAGTGGTTTTATATTTTTCAGCAGAATGTCTACCATCAGCAACTCAACAAATATATTTTGGCAAGAATGTCCTGTTGGGAAAGCTGAAAGGCAGAAACTACTCAACCAAAAGGGCTGTGTTGTATGGATTACAGGTCTCAGCGGATCAGGGAAAAGTACACTGGCATGCTCACTAAGTAGAGAACTGCACTCGAGGGGAAAGCTGTCATATATCCTTGATGGTGATAACCTTCGACATGGTATCAACAAGGATCTTGGTTTCAAACCAGAAGACCGGACTGAAAATATTCGCAGAGTTGGGGAGATCTCAAAACTGTTTGCGGATGCAGGATTGATCTGTATTGCTAGTCTGATATCTCCTTATAGGAAAGATCGTGATGCTTGCCGTGCAATGCTACCAGATGCAAATTTTATTGAGGTTTTCATGAACATGCCCCTAGAGTTATGTGAGTCAAGGGATGCAAAAGGCCTTTACAAGCTTGCACGTGCGGGAAAGATTAGAGGTTTTACTGGAATTGATGATCCATATGAACAACCACTGAACTGTGAGATAGAAATACAGCAAAATAATGGAGCTTGTCCCCTGCCTGCTGTCCTGGCAGGCCAGGTAGTGACATACTTGGAGGAGAAAGGCTTTCTTCAAGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00920 ko01100 ko01120 map00230 map00920 map01100 map01120
Pfam Domains
Protein Families
APS_kinase

Protein Analysis

294

Amino Acids

32.69

Weight (kDa)

8.63

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 114 - 263 1.2e-67 Adenylylsulphate kinase
AAA_33 PF13671 117 - 232 9.5e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 174
AccI GTMKAC 1 cut(s) 260
AccII CGCG 1 cut(s) 19
AciI CCGC 3 cut(s) 372, 551, 719
AclWI GGATC 3 cut(s) 382, 489, 746
AcsI RAATTY 1 cut(s) 637
AcvI CACGTG 1 cut(s) 716
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 3 cut(s) 154, 493, 872
AjnI CCWGG 2 cut(s) 825, 834
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 5 cut(s) 80, 314, 433, 709, 806
AluI AGCT 5 cut(s) 80, 314, 433, 709, 806
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 3 cut(s) 382, 489, 746
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 3 cut(s) 214, 697, 832
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 1 cut(s) 637
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
Asp700I GAANNNNTTC 2 cut(s) 182, 516
AspLEI GCGC 1 cut(s) 21
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 17, 464
AvaI CYCGRG 1 cut(s) 421
BbrPI CACGTG 1 cut(s) 716
BbsI GAAGAC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 57
BccI CCATC 3 cut(s) 212, 272, 443
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 2 cut(s) 827, 836
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 576, 668
BglII AGATCT 1 cut(s) 534
BisI GCNGC 1 cut(s) 46
BlsI GCNGC 1 cut(s) 47
Bme1390I CCNGG 3 cut(s) 213, 827, 836
BmeT110I CYCGRG 1 cut(s) 421
BmrFI CCNGG 3 cut(s) 213, 827, 836
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 5 cut(s) 94, 544, 598, 622, 679
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 716
BsaI GGTCTC 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 504
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 3 cut(s) 59, 396, 745
Bse3DI GCAATG 2 cut(s) 92, 627
BseBI CCWGG 2 cut(s) 827, 836
BseGI GGATG 4 cut(s) 109, 204, 559, 694
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 2 cut(s) 92, 627
BseMII CTCAG 1 cut(s) 382
BseNI ACTGG 3 cut(s) 59, 396, 745
BseRI GAGGAG 2 cut(s) 113, 869
BseXI GCAGC 1 cut(s) 57
BsgI GTGCAG 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 3 cut(s) 216, 699, 834
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 2 cut(s) 213, 505
BslFI GGGAC 1 cut(s) 795
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 795
BsnI GGCC 3 cut(s) 216, 699, 834
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 421
Bsp143I GATC 6 cut(s) 374, 481, 534, 564, 601, 751
BspACI CCGC 3 cut(s) 372, 551, 719
BspANI GGCC 3 cut(s) 216, 699, 834
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 654
BspPI GGATC 3 cut(s) 382, 489, 746
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 2 cut(s) 92, 627
BsrI ACTGG 3 cut(s) 59, 396, 745
BssMI GATC 6 cut(s) 374, 481, 534, 564, 601, 751
Bst2UI CCWGG 2 cut(s) 827, 836
Bst4CI ACNGT 2 cut(s) 546, 776
Bst6I CTCTTC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 6 cut(s) 61, 397, 613, 711, 819, 832
BstDEI CTNAG 2 cut(s) 368, 404
BstF5I GGATG 4 cut(s) 109, 204, 559, 694
BstFNI CGCG 1 cut(s) 19
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 6 cut(s) 377, 484, 537, 567, 604, 754
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 6 cut(s) 374, 481, 534, 564, 601, 751
BstMWI GCNNNNNNNGC 2 cut(s) 617, 827
BstNI CCWGG 2 cut(s) 827, 836
BstNSI RCATGY 3 cut(s) 63, 399, 664
BstSCI CCNGG 3 cut(s) 211, 825, 834
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 2 cut(s) 481, 534
BstYI RGATCY 2 cut(s) 481, 534
BsuRI GGCC 3 cut(s) 216, 699, 834
BtsCI GGATG 4 cut(s) 109, 204, 559, 694
BtsIMutI CAGTG 3 cut(s) 238, 389, 767
Cac8I GCNNGC 6 cut(s) 61, 397, 613, 711, 819, 832
CciI TCATGA 1 cut(s) 654
CfoI GCGC 1 cut(s) 21
CseI GACGC 2 cut(s) 25, 96
Csp6I GTAC 1 cut(s) 387
CviAII CATG 5 cut(s) 60, 396, 467, 655, 661
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 368, 404
DpnI GATC 6 cut(s) 376, 483, 536, 566, 603, 753
DpnII GATC 6 cut(s) 374, 481, 534, 564, 601, 751
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 585
Eco72I CACGTG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 825, 834
EcoRV GATATC 1 cut(s) 585
FaeI CATG 5 cut(s) 63, 399, 470, 658, 664
FalI AAGNNNNNCTT 3 cut(s) 134, 166, 852
FaqI GGGAC 1 cut(s) 795
FatI CATG 5 cut(s) 59, 395, 466, 654, 660
FauI CCCGC 1 cut(s) 712
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 1 cut(s) 46
FokI GGATG 4 cut(s) 116, 191, 566, 701
Fsp4HI GCNGC 1 cut(s) 46
FspBI CTAG 2 cut(s) 576, 668
GlaI GCGC 1 cut(s) 20
GluI GCNGC 1 cut(s) 46
HaeIII GGCC 3 cut(s) 216, 699, 834
HapII CCGG 2 cut(s) 213, 505
HgaI GACGC 2 cut(s) 25, 96
HhaI GCGC 1 cut(s) 21
Hin1II CATG 5 cut(s) 63, 399, 470, 658, 664
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 2 cut(s) 114, 680
HpaII CCGG 2 cut(s) 213, 505
HphI GGTGA 2 cut(s) 17, 464
Hpy166II GTNNAC 2 cut(s) 261, 389
Hpy188I TCNGA 1 cut(s) 582
Hpy188III TCNNGA 3 cut(s) 111, 605, 655
Hpy8I GTNNAC 2 cut(s) 261, 389
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 85, 470
HpyCH4III ACNGT 2 cut(s) 546, 776
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 7 cut(s) 45, 418, 557, 620, 635, 692, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 827
HpyF3I CTNAG 2 cut(s) 368, 404
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 5 cut(s) 63, 399, 470, 658, 664
HspAI GCGC 1 cut(s) 19
Kzo9I GATC 6 cut(s) 374, 481, 534, 564, 601, 751
LmnI GCTCC 1 cut(s) 803
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 5 cut(s) 94, 544, 598, 622, 679
MaeI CTAG 2 cut(s) 576, 668
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 1 cut(s) 841
MalI GATC 6 cut(s) 376, 483, 536, 566, 603, 753
MboI GATC 6 cut(s) 374, 481, 534, 564, 601, 751
MboII GAAGA 4 cut(s) 107, 184, 512, 860
MflI RGATCY 2 cut(s) 481, 534
MluCI AATT 3 cut(s) 221, 637, 744
MlyI GAGTC 1 cut(s) 689
MnlI CCTC 9 cut(s) 84, 91, 94, 219, 227, 417, 640, 725, 847
MroXI GAANNNNTTC 2 cut(s) 182, 516
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 3 cut(s) 30, 394, 659
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 2 cut(s) 213, 505
MspR9I CCNGG 3 cut(s) 213, 827, 836
MvaI CCWGG 2 cut(s) 827, 836
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 2 cut(s) 617, 827
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 757
NdeII GATC 6 cut(s) 374, 481, 534, 564, 601, 751
NlaIII CATG 5 cut(s) 63, 399, 470, 658, 664
NmuCI GTSAC 1 cut(s) 841
NspI RCATGY 3 cut(s) 63, 399, 664
PaeI GCATGC 2 cut(s) 63, 399
PaeR7I CTCGAG 1 cut(s) 421
PagI TCATGA 1 cut(s) 654
PceI AGGCCT 1 cut(s) 699
PdmI GAANNNNTTC 2 cut(s) 182, 516
PfeI GAWTC 1 cut(s) 114
PflMI CCANNNNNTGG 1 cut(s) 174
PkrI GCNGC 1 cut(s) 47
PleI GAGTC 1 cut(s) 688
PmaCI CACGTG 1 cut(s) 716
PmlI CACGTG 1 cut(s) 716
PpsI GAGTC 1 cut(s) 688
Ppu21I YACGTR 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 825, 834
PspCI CACGTG 1 cut(s) 716
PspGI CCWGG 2 cut(s) 825, 834
PspXI VCTCGAGB 1 cut(s) 421
PsuI RGATCY 2 cut(s) 481, 534
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 3 cut(s) 30, 394, 659
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 1 cut(s) 46
Sau3AI GATC 6 cut(s) 374, 481, 534, 564, 601, 751
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 3 cut(s) 213, 827, 836
SfaNI GCATC 5 cut(s) 94, 544, 598, 622, 679
Sfr274I CTCGAG 1 cut(s) 421
SlaI CTCGAG 1 cut(s) 421
SmiMI CAYNNNNRTG 3 cut(s) 30, 394, 659
SmlI CTYRAG 2 cut(s) 75, 421
SmoI CTYRAG 2 cut(s) 75, 421
SphI GCATGC 2 cut(s) 63, 399
Sse9I AATT 3 cut(s) 221, 637, 744
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 3 cut(s) 372, 551, 719
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 576, 668
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 211, 825, 834
TaaI ACNGT 2 cut(s) 546, 776
TaiI ACGT 1 cut(s) 718
TaqI TCGA 3 cut(s) 219, 422, 463
TasI AATT 3 cut(s) 221, 637, 744
TatI WGTACW 1 cut(s) 386
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 3 cut(s) 238, 396, 774
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 4 cut(s) 106, 643, 671, 774
TspRI CASTG 3 cut(s) 238, 396, 774
Van91I CCANNNNNTGG 1 cut(s) 174
XapI RAATTY 1 cut(s) 637
XceI RCATGY 3 cut(s) 63, 399, 664
XhoI CTCGAG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 260
XmnI GAANNNNTTC 2 cut(s) 182, 516
XspI CTAG 2 cut(s) 576, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.