MD12G1004900.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016574
Physical Location & Seq
Reverse (-)
620608 .. 624398
3791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1004900.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGAGTTGATAGCAGAAAAGGAGTCGAACCACAACAACAATGAGAACAACAACAACAACAACGTCACCAGTTACGCGCAAGCACTCGTCGTAGACTCTCACGATAATCATCCCAAGGAATCCGATGAAGTTCGTGAACGCCATGCTCGCGACCTCAAAGCTGGCCTCCATCCCCTCAAGCACAAGTTCGTATTTTGGTACACTCGTCGCACCCCAGGAATCCGGAGCCAGACACCATATGAAGACAATATAAAGAAAATTGTCGACTTCAGTACGGTTGAAGATTTTTGGGTCTGTTACTGCCACCTGGCCCGTCCTTCTTCTTTGCCTAGTCCAACAGACTTGCATCTTTTCAAAGATGGAATCCGCCCGCTCTGGGAAGACTCTGCTAATTGCAATGGTGGTAAATGGATAATACGATTCAAAAAGGCTGTGTCAGGCCGTTTTTGGGAGGACCTGGTTCTTGCCTTGGTGGGTGACCAGCTTGATTATGGAGAGAACATATGTGGTGCAGTACTAAGCATCAGATTCAATGAGGATATATTGAGTGTCTGGAATCGGAATGCTTCTGATCATCAGGCTGTAATGGCTTTAAGAGATGCGATCAAGCGGAACTTGAAGCTTCCACACAGCTATCTCATGGAATACAAGCCACACGATGCTTCGCTGCGCGACAATTCATCTTACAGAAACACATGGTTGAGGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko03013 ko04066 ko04150 ko04151 ko04211 ko04910 map01521 map03013 map04066 map04150 map04151 map04211 map04910
Pfam Domains
Protein Families
IF4E

Protein Analysis

237

Amino Acids

27.36

Weight (kDa)

6.83

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 58 - 216 8.7e-56 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18110
fragaria_vesca FvH4_1g28260
malus_domestica MD12G1004900.v1.1 MD14G1003400.v1.1
prunus_persica Prupe.7G005900_v2.0.a1
pyrus_communis pycom12g00400 pycom14g00140
rosa_chinensis RchiOBHm_Chr3g0496901
rosa_laevigata RLG00000022491
rosa_roxburghii Rroxscaffold_6G00388600
rosa_rugosa Rorug03G0288400
rosa_samantha Rh3AG332300 Rh3DG368300
rosa_wichuraiana Rw3G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 2 cut(s) 93, 264
AccII CGCG 3 cut(s) 77, 150, 672
AccIII TCCGGA 1 cut(s) 222
AciI CCGC 3 cut(s) 367, 371, 610
AcuI CTGAAG 1 cut(s) 253
AfaI GTAC 3 cut(s) 200, 274, 516
AfiI CCNNNNNNNGG 2 cut(s) 376, 448
AgsI TTSAA 5 cut(s) 281, 355, 424, 532, 619
AjnI CCWGG 3 cut(s) 214, 306, 456
AluBI AGCT 4 cut(s) 161, 484, 622, 633
AluI AGCT 4 cut(s) 161, 484, 622, 633
Aor13HI TCCGGA 1 cut(s) 222
AoxI GGCC 3 cut(s) 163, 309, 439
ApeKI GCWGC 1 cut(s) 667
AspLEI GCGC 2 cut(s) 79, 672
AspS9I GGNCC 2 cut(s) 310, 454
AsuHPI GGTGA 2 cut(s) 58, 488
AvaII GGWCC 1 cut(s) 454
BaeI ACNNNNGTAYC 2 cut(s) 190, 223
BbsI GAAGAC 2 cut(s) 249, 387
BbvI GCAGC 1 cut(s) 654
BccI CCATC 2 cut(s) 177, 353
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 3 cut(s) 216, 308, 458
BclI TGATCA 1 cut(s) 571
BfaI CTAG 2 cut(s) 330, 709
BisI GCNGC 1 cut(s) 668
BlsI GCNGC 1 cut(s) 669
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 3 cut(s) 216, 308, 458
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 310, 454
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 3 cut(s) 216, 308, 458
BmsI GCATC 4 cut(s) 355, 531, 589, 649
BpiI GAAGAC 2 cut(s) 249, 387
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 114, 214, 468
BsaWI WCCGGW 1 cut(s) 222
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 448
Bse1I ACTGG 1 cut(s) 69
Bse3DI GCAATG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 222
BseBI CCWGG 3 cut(s) 216, 308, 458
BseDI CCNNGG 3 cut(s) 114, 214, 468
BseGI GGATG 2 cut(s) 109, 169
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 376, 448
BseMI GCAATG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 654
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 3 cut(s) 77, 150, 672
BshFI GGCC 3 cut(s) 165, 311, 441
BsiSI CCGG 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 376, 448
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 3 cut(s) 165, 311, 441
Bsp13I TCCGGA 1 cut(s) 222
Bsp143I GATC 2 cut(s) 571, 603
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 3 cut(s) 367, 371, 610
BspANI GGCC 3 cut(s) 165, 311, 441
BspEI TCCGGA 1 cut(s) 222
BspFNI CGCG 3 cut(s) 77, 150, 672
BspLI GGNNCC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 403
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 3 cut(s) 114, 214, 468
BssMI GATC 2 cut(s) 571, 603
BssT1I CCWWGG 2 cut(s) 114, 468
Bst2UI CCWGG 3 cut(s) 216, 308, 458
Bst4CI ACNGT 1 cut(s) 277
BstC8I GCNNGC 4 cut(s) 81, 148, 163, 371
BstDEI CTNAG 1 cut(s) 518
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 2 cut(s) 109, 169
BstFNI CGCG 3 cut(s) 77, 150, 672
BstHHI GCGC 2 cut(s) 79, 672
BstKTI GATC 2 cut(s) 574, 606
BstMBI GATC 2 cut(s) 571, 603
BstMWI GCNNNNNNNGC 2 cut(s) 147, 587
BstNI CCWGG 3 cut(s) 216, 308, 458
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 3 cut(s) 214, 306, 456
BstUI CGCG 3 cut(s) 77, 150, 672
BstV1I GCAGC 1 cut(s) 654
BstV2I GAAGAC 2 cut(s) 249, 387
BsuRI GGCC 3 cut(s) 165, 311, 441
BtsCI GGATG 2 cut(s) 109, 169
BtuMI TCGCGA 1 cut(s) 150
Cac8I GCNNGC 4 cut(s) 81, 148, 163, 371
CfoI GCGC 2 cut(s) 79, 672
Cfr13I GGNCC 2 cut(s) 310, 454
CsiI ACCWGGT 1 cut(s) 456
Csp6I GTAC 3 cut(s) 199, 273, 515
CviAII CATG 3 cut(s) 143, 640, 696
CviQI GTAC 3 cut(s) 199, 273, 515
DdeI CTNAG 1 cut(s) 518
DpnI GATC 2 cut(s) 573, 605
DpnII GATC 2 cut(s) 571, 603
EciI GGCGGA 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 114, 468
Eco47I GGWCC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 253
Eco91I GGTNACC 1 cut(s) 476
EcoO109I RGGNCCY 1 cut(s) 454
EcoO65I GGTNACC 1 cut(s) 476
EcoRII CCWGG 3 cut(s) 214, 306, 456
EcoT14I CCWWGG 2 cut(s) 114, 468
ErhI CCWWGG 2 cut(s) 114, 468
FaeI CATG 3 cut(s) 146, 643, 699
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FatI CATG 3 cut(s) 142, 639, 695
FauI CCCGC 1 cut(s) 378
FauNDI CATATG 2 cut(s) 238, 503
FbaI TGATCA 1 cut(s) 571
FblI GTMKAC 2 cut(s) 93, 264
Fnu4HI GCNGC 1 cut(s) 668
FokI GGATG 2 cut(s) 96, 156
Fsp4HI GCNGC 1 cut(s) 668
FspBI CTAG 2 cut(s) 330, 709
GlaI GCGC 2 cut(s) 78, 671
GluI GCNGC 1 cut(s) 668
HaeIII GGCC 3 cut(s) 165, 311, 441
HapII CCGG 1 cut(s) 223
HhaI GCGC 2 cut(s) 79, 672
Hin1II CATG 3 cut(s) 146, 643, 699
Hin6I GCGC 2 cut(s) 77, 670
HinP1I GCGC 2 cut(s) 77, 670
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 9 cut(s) 23, 95, 119, 219, 363, 383, 420, 528, 556
HpaII CCGG 1 cut(s) 223
HphI GGTGA 2 cut(s) 58, 488
Hpy166II GTNNAC 4 cut(s) 94, 137, 201, 265
Hpy188I TCNGA 4 cut(s) 124, 527, 561, 571
Hpy188III TCNNGA 5 cut(s) 101, 134, 149, 223, 553
Hpy8I GTNNAC 4 cut(s) 94, 137, 201, 265
Hpy99I CGWCG 2 cut(s) 92, 210
HpyAV CCTTC 1 cut(s) 327
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 346, 396, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 587
HpyF3I CTNAG 1 cut(s) 518
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 3 cut(s) 146, 643, 699
HspAI GCGC 2 cut(s) 77, 670
Kpn2I TCCGGA 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 2 cut(s) 571, 603
LmnI GCTCC 1 cut(s) 225
Lsp1109I GCAGC 1 cut(s) 654
LweI GCATC 4 cut(s) 355, 531, 589, 649
MabI ACCWGGT 1 cut(s) 456
MaeI CTAG 2 cut(s) 330, 709
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 4 cut(s) 64, 71, 296, 476
MalI GATC 2 cut(s) 573, 605
MbiI CCGCTC 1 cut(s) 373
MboI GATC 2 cut(s) 571, 603
MboII GAAGA 4 cut(s) 254, 293, 312, 392
MluCI AATT 3 cut(s) 258, 391, 676
MlyI GAGTC 3 cut(s) 32, 89, 377
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 6 cut(s) 164, 176, 185, 445, 529, 696
MroI TCCGGA 1 cut(s) 222
MseI TTAA 1 cut(s) 593
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 3 cut(s) 216, 308, 458
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 3 cut(s) 216, 308, 458
MvnI CGCG 3 cut(s) 77, 150, 672
MwoI GCNNNNNNNGC 2 cut(s) 147, 587
NdeI CATATG 2 cut(s) 238, 503
NdeII GATC 2 cut(s) 571, 603
NlaIII CATG 3 cut(s) 146, 643, 699
NlaIV GGNNCC 1 cut(s) 227
NmuCI GTSAC 2 cut(s) 64, 476
NruI TCGCGA 1 cut(s) 150
PctI GAATGC 1 cut(s) 568
PfeI GAWTC 6 cut(s) 119, 219, 363, 420, 528, 556
PkrI GCNGC 1 cut(s) 669
PleI GAGTC 3 cut(s) 31, 89, 377
PpsI GAGTC 3 cut(s) 31, 89, 377
PpuMI RGGWCCY 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 3 cut(s) 214, 306, 456
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 3 cut(s) 214, 306, 456
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 2 cut(s) 310, 454
PspPPI RGGWCCY 1 cut(s) 454
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 3 cut(s) 200, 274, 516
RsaNI GTAC 3 cut(s) 199, 273, 515
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 593
SatI GCNGC 1 cut(s) 668
Sau3AI GATC 2 cut(s) 571, 603
Sau96I GGNCC 2 cut(s) 310, 454
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 3 cut(s) 32, 89, 377
ScrFI CCNGG 3 cut(s) 216, 308, 458
SetI ASST 8 cut(s) 66, 156, 163, 309, 459, 486, 624, 635
SexAI ACCWGGT 1 cut(s) 456
SfaNI GCATC 4 cut(s) 355, 531, 589, 649
SinI GGWCC 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 258, 391, 676
SsiI CCGC 3 cut(s) 367, 371, 610
SspMI CTAG 2 cut(s) 330, 709
StyD4I CCNGG 3 cut(s) 214, 306, 456
StyI CCWWGG 2 cut(s) 114, 468
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 26, 264
TasI AATT 3 cut(s) 258, 391, 676
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 6 cut(s) 119, 219, 363, 420, 528, 556
Tru1I TTAA 1 cut(s) 593
Tru9I TTAA 1 cut(s) 593
TseFI GTSAC 2 cut(s) 64, 476
TseI GCWGC 1 cut(s) 667
Tsp45I GTSAC 2 cut(s) 64, 476
TspDTI ATGAA 3 cut(s) 141, 255, 669
VpaK11BI GGWCC 1 cut(s) 454
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmiI GTMKAC 2 cut(s) 93, 264
XspI CTAG 2 cut(s) 330, 709
ZrmI AGTACT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.