MD14G1003400.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016574
Physical Location & Seq
Reverse (-)
414478 .. 419057
4580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1003400.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGAGTTGATAGCAGAAAAGGAGTCGAACCACAACAACAATGAGAACAACAACAACAACGTCACCAGTTACGCGCAAGCACTCTTAGACTCTCGCGATAATCATCCCAACGAATCCGATGAAGTTCGTGAACGTCATGCTCGCGACCTCAAAGCTGGCCTCCATCCTCTCAAGCACAAGTTCGTATTTTGGTACACTCGCCGAACTCCAGGAATCCGGAGCCAGACATCATATGAAGACAATATAAAGAAAATAGTCGACTTCAGTACGGTTGAAGGTTTTTGGGTCAGTTATTGCCACCTAGCCCGACCTTCTTCTTTGCCTAGTCCAACAGACTTGCATCTTTTCAAGGATGGAATTCGCCCTCTCTGGGAGGACTCTGCTAATTGCAACGGTGGCAAATGGATAATACGATTCAAAAAGGCTGTGTCGGGCCGTTTTTGGGAGGACCTGGTTCTTGCCTTGGTGGGTGACCAGCTTGATTATGGAGAGAACATATGTGGTGCGGTACTAAGCATCAGATTCAATGAGGATATATTGAGTGTCTGGAACCGGAATGCTTCTGATCATCAGGCTGTAATGGCTTTAAGAGATGCGATCAAGCGGAACTTGAAGCTTCCACACAGCTATCTCATGGAATACAAGCCACATGATGCTTCGCTGCGCGACAATTCATCTTACAGAAACACATGGTTGAGGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko03013 ko04066 ko04150 ko04151 ko04211 ko04910 map01521 map03013 map04066 map04150 map04151 map04211 map04910
Pfam Domains
Protein Families
IF4E

Protein Analysis

235

Amino Acids

27.08

Weight (kDa)

7.16

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 56 - 214 3.1e-55 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18110
fragaria_vesca FvH4_1g28260
malus_domestica MD12G1004900.v1.1 MD14G1003400.v1.1
prunus_persica Prupe.7G005900_v2.0.a1
pyrus_communis pycom12g00400 pycom14g00140
rosa_chinensis RchiOBHm_Chr3g0496901
rosa_laevigata RLG00000022491
rosa_roxburghii Rroxscaffold_6G00388600
rosa_rugosa Rorug03G0288400
rosa_samantha Rh3AG332300 Rh3DG368300
rosa_wichuraiana Rw3G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AccII CGCG 4 cut(s) 74, 96, 144, 666
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 2 cut(s) 506, 604
AcsI RAATTY 1 cut(s) 357
AcuI CTGAAG 1 cut(s) 247
AfaI GTAC 3 cut(s) 194, 268, 510
AfiI CCNNNNNNNGG 2 cut(s) 370, 442
AgsI TTSAA 5 cut(s) 275, 349, 418, 526, 613
AjnI CCWGG 2 cut(s) 208, 450
AluBI AGCT 4 cut(s) 155, 478, 616, 627
AluI AGCT 4 cut(s) 155, 478, 616, 627
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 2 cut(s) 157, 433
ApeKI GCWGC 1 cut(s) 661
ApoI RAATTY 1 cut(s) 357
AspLEI GCGC 2 cut(s) 76, 666
AspS9I GGNCC 2 cut(s) 433, 448
AsuHPI GGTGA 2 cut(s) 55, 482
AvaII GGWCC 1 cut(s) 448
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 648
BccI CCATC 2 cut(s) 171, 347
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 2 cut(s) 210, 452
BclI TGATCA 1 cut(s) 565
BfaI CTAG 3 cut(s) 302, 324, 703
BisI GCNGC 1 cut(s) 662
BlsI GCNGC 1 cut(s) 663
Bme1390I CCNGG 2 cut(s) 210, 452
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 433, 448
BmiI GGNNCC 2 cut(s) 221, 551
BmrFI CCNGG 2 cut(s) 210, 452
BmsI GCATC 4 cut(s) 349, 525, 583, 643
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 462
BsaWI WCCGGW 2 cut(s) 216, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 442
Bse1I ACTGG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 2 cut(s) 210, 452
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 3 cut(s) 103, 163, 358
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 370, 442
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 648
Bsh1236I CGCG 4 cut(s) 74, 96, 144, 666
BshFI GGCC 2 cut(s) 159, 435
BsiSI CCGG 2 cut(s) 217, 553
BslI CCNNNNNNNGG 2 cut(s) 370, 442
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 2 cut(s) 159, 435
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 2 cut(s) 565, 597
Bsp68I TCGCGA 2 cut(s) 96, 144
BspACI CCGC 2 cut(s) 506, 604
BspANI GGCC 2 cut(s) 159, 435
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 4 cut(s) 74, 96, 144, 666
BspLI GGNNCC 2 cut(s) 221, 551
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 2 cut(s) 565, 597
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 210, 452
Bst4CI ACNGT 2 cut(s) 271, 395
BstC8I GCNNGC 3 cut(s) 78, 142, 157
BstDEI CTNAG 2 cut(s) 85, 512
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 3 cut(s) 103, 163, 358
BstFNI CGCG 4 cut(s) 74, 96, 144, 666
BstHHI GCGC 2 cut(s) 76, 666
BstKTI GATC 2 cut(s) 568, 600
BstMBI GATC 2 cut(s) 565, 597
BstMWI GCNNNNNNNGC 2 cut(s) 396, 581
BstNI CCWGG 2 cut(s) 210, 452
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 208, 450
BstUI CGCG 4 cut(s) 74, 96, 144, 666
BstV1I GCAGC 1 cut(s) 648
BstV2I GAAGAC 1 cut(s) 243
BsuRI GGCC 2 cut(s) 159, 435
BtsCI GGATG 3 cut(s) 103, 163, 358
BtuMI TCGCGA 2 cut(s) 96, 144
Cac8I GCNNGC 3 cut(s) 78, 142, 157
CfoI GCGC 2 cut(s) 76, 666
Cfr13I GGNCC 2 cut(s) 433, 448
CsiI ACCWGGT 1 cut(s) 450
Csp6I GTAC 3 cut(s) 193, 267, 509
CviAII CATG 4 cut(s) 137, 634, 650, 690
CviQI GTAC 3 cut(s) 193, 267, 509
DdeI CTNAG 2 cut(s) 85, 512
DpnI GATC 2 cut(s) 567, 599
DpnII GATC 2 cut(s) 565, 597
Eco130I CCWWGG 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 247
Eco91I GGTNACC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 448
EcoO65I GGTNACC 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 208, 450
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 4 cut(s) 140, 637, 653, 693
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FatI CATG 4 cut(s) 136, 633, 649, 689
FauNDI CATATG 2 cut(s) 232, 497
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 662
FokI GGATG 3 cut(s) 90, 150, 365
Fsp4HI GCNGC 1 cut(s) 662
FspBI CTAG 3 cut(s) 302, 324, 703
GlaI GCGC 2 cut(s) 75, 665
GluI GCNGC 1 cut(s) 662
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 2 cut(s) 159, 435
HapII CCGG 2 cut(s) 217, 553
HhaI GCGC 2 cut(s) 76, 666
Hin1II CATG 4 cut(s) 140, 637, 653, 693
Hin6I GCGC 2 cut(s) 74, 664
HinP1I GCGC 2 cut(s) 74, 664
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 7 cut(s) 23, 89, 113, 213, 377, 414, 522
HpaII CCGG 2 cut(s) 217, 553
HphI GGTGA 2 cut(s) 55, 482
Hpy166II GTNNAC 3 cut(s) 131, 195, 259
Hpy188I TCNGA 3 cut(s) 118, 521, 565
Hpy188III TCNNGA 5 cut(s) 95, 128, 143, 217, 547
Hpy8I GTNNAC 3 cut(s) 131, 195, 259
HpyAV CCTTC 2 cut(s) 269, 321
HpyCH4III ACNGT 2 cut(s) 271, 395
HpyCH4IV ACGT 2 cut(s) 60, 133
HpyCH4V TGCA 2 cut(s) 340, 390
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 581
HpyF3I CTNAG 2 cut(s) 85, 512
HpySE526I ACGT 2 cut(s) 60, 133
Hsp92II CATG 4 cut(s) 140, 637, 653, 693
HspAI GCGC 2 cut(s) 74, 664
Kpn2I TCCGGA 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 2 cut(s) 565, 597
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 648
LweI GCATC 4 cut(s) 349, 525, 583, 643
MabI ACCWGGT 1 cut(s) 450
MaeI CTAG 3 cut(s) 302, 324, 703
MaeII ACGT 2 cut(s) 60, 133
MaeIII GTNAC 3 cut(s) 61, 68, 470
MalI GATC 2 cut(s) 567, 599
MboI GATC 2 cut(s) 565, 597
MboII GAAGA 2 cut(s) 248, 306
MluCI AATT 3 cut(s) 357, 385, 670
MlyI GAGTC 3 cut(s) 32, 83, 371
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 8 cut(s) 158, 170, 177, 367, 375, 439, 523, 690
MroI TCCGGA 1 cut(s) 216
MseI TTAA 1 cut(s) 587
MspI CCGG 2 cut(s) 217, 553
MspR9I CCNGG 2 cut(s) 210, 452
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 2 cut(s) 210, 452
MvnI CGCG 4 cut(s) 74, 96, 144, 666
MwoI GCNNNNNNNGC 2 cut(s) 396, 581
NdeI CATATG 2 cut(s) 232, 497
NdeII GATC 2 cut(s) 565, 597
NlaIII CATG 4 cut(s) 140, 637, 653, 693
NlaIV GGNNCC 2 cut(s) 221, 551
NmuCI GTSAC 2 cut(s) 61, 470
NruI TCGCGA 2 cut(s) 96, 144
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 113, 213, 414, 522
PfoI TCCNGGA 1 cut(s) 208
PkrI GCNGC 1 cut(s) 663
PleI GAGTC 3 cut(s) 31, 83, 371
PpsI GAGTC 3 cut(s) 31, 83, 371
PpuMI RGGWCCY 1 cut(s) 448
Psp5II RGGWCCY 1 cut(s) 448
Psp6I CCWGG 2 cut(s) 208, 450
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 208, 450
PspN4I GGNNCC 2 cut(s) 221, 551
PspPI GGNCC 2 cut(s) 433, 448
PspPPI RGGWCCY 1 cut(s) 448
RruI TCGCGA 2 cut(s) 96, 144
RsaI GTAC 3 cut(s) 194, 268, 510
RsaNI GTAC 3 cut(s) 193, 267, 509
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 1 cut(s) 662
Sau3AI GATC 2 cut(s) 565, 597
Sau96I GGNCC 2 cut(s) 433, 448
SchI GAGTC 3 cut(s) 32, 83, 371
ScrFI CCNGG 2 cut(s) 210, 452
SexAI ACCWGGT 1 cut(s) 450
SfaNI GCATC 4 cut(s) 349, 525, 583, 643
SinI GGWCC 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 3 cut(s) 357, 385, 670
SsiI CCGC 2 cut(s) 506, 604
SspMI CTAG 3 cut(s) 302, 324, 703
StyD4I CCNGG 2 cut(s) 208, 450
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 2 cut(s) 271, 395
TaiI ACGT 2 cut(s) 63, 136
TaqI TCGA 2 cut(s) 26, 258
TasI AATT 3 cut(s) 357, 385, 670
TfiI GAWTC 4 cut(s) 113, 213, 414, 522
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TseFI GTSAC 2 cut(s) 61, 470
TseI GCWGC 1 cut(s) 661
Tsp45I GTSAC 2 cut(s) 61, 470
TspDTI ATGAA 3 cut(s) 135, 249, 663
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 3 cut(s) 302, 324, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.