pycom12g00400

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016574
Physical Location & Seq
Reverse (-)
404003 .. 407978
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00400.2

Sequence Viewer

Length: 780 bp
ATGAGATATGAGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAATTAGGGATTTTGGGGAGCATGGAGTTGATAGCAGAAAAGGAGTCGAATAACAACAACAATGAGAACAACAACAACGTCACCAGTTACGCGCAAGCACTCGTCGTAGACTCTCACGATAATCATCCCAAGGAATCCGATGAAGTCCGTGAACGCCATGCTCGCGACCTCAAAGCTGGCCTCCATCCCCTCAAGCACAAGTTCGTATTTTGGTACACTCGTCGCACCCCAGGAATCCGGAGCCAGACACCATATGAAGACAATATAAAGAAAATTGTCGACTTCAGTACGGTTGAAGATTTTTGGGTCTGTTACTGCCACCTAGCCCGTCCTTCTTCTTTGCCTAGTCCAACAGACTTGCATCTTTTCAAAGATGGAATCCGCCCGCTCTGGGAGGACTCTGCTAATTGCAATGGTGGTAAATGGATAATACGATTCAAAAAGGCTGTTTCGGGCCGTTTTTGGGAGGACCTGGTTCTTGCCTTGGTGGGTGACCAGCTTGATTATGGAGAGAACATATGTGGTGCAGTACTAAGCATCAGATTCAATGAGGATATATTGAGTGTCTGGAATCGGAATGCTTCTGATCATCAGGCTGTAATGGCTTTAAGAGATGCGATCAAGCGGAACTTGAAGCTTCCACACAGCTATCTCATGGAATACAAGCCACACGATGCTTCGCTGCGTGACAATTCATCTTACAGAAACACATGGTTGAGGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko03013 ko04066 ko04150 ko04151 ko04211 ko04910 map01521 map03013 map04066 map04150 map04151 map04211 map04910
Pfam Domains
Protein Families
IF4E

Protein Analysis

260

Amino Acids

30.36

Weight (kDa)

6.57

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18110
fragaria_vesca FvH4_1g28260
malus_domestica MD12G1004900.v1.1 MD14G1003400.v1.1
prunus_persica Prupe.7G005900_v2.0.a1
pyrus_communis pycom12g00400 pycom14g00140
rosa_chinensis RchiOBHm_Chr3g0496901
rosa_laevigata RLG00000022491
rosa_roxburghii Rroxscaffold_6G00388600
rosa_rugosa Rorug03G0288400
rosa_samantha Rh3AG332300 Rh3DG368300
rosa_wichuraiana Rw3G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 442
AccI GTMKAC 2 cut(s) 162, 333
AccII CGCG 2 cut(s) 146, 219
AccIII TCCGGA 1 cut(s) 291
AciI CCGC 3 cut(s) 436, 440, 679
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 3 cut(s) 269, 343, 585
AfiI CCNNNNNNNGG 2 cut(s) 445, 517
AgsI TTSAA 5 cut(s) 350, 424, 493, 601, 688
AjnI CCWGG 2 cut(s) 283, 525
AluBI AGCT 4 cut(s) 230, 553, 691, 702
AluI AGCT 4 cut(s) 230, 553, 691, 702
Aor13HI TCCGGA 1 cut(s) 291
AoxI GGCC 2 cut(s) 232, 508
ApeKI GCWGC 1 cut(s) 736
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 508, 523
AsuHPI GGTGA 2 cut(s) 127, 557
AvaII GGWCC 1 cut(s) 523
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 723
BccI CCATC 2 cut(s) 246, 422
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 2 cut(s) 285, 527
BclI TGATCA 1 cut(s) 640
BfaI CTAG 2 cut(s) 377, 399
BisI GCNGC 1 cut(s) 737
BlsI GCNGC 1 cut(s) 738
BmcAI AGTACT 1 cut(s) 585
Bme1390I CCNGG 2 cut(s) 285, 527
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 508, 523
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 2 cut(s) 285, 527
BmsI GCATC 4 cut(s) 424, 600, 658, 718
BpiI GAAGAC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 183, 283, 537
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 517
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 472
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 291
BseBI CCWGG 2 cut(s) 285, 527
BseDI CCNNGG 3 cut(s) 183, 283, 537
BseGI GGATG 2 cut(s) 178, 238
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 445, 517
BseMI GCAATG 1 cut(s) 472
BseNI ACTGG 1 cut(s) 138
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 723
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 2 cut(s) 146, 219
BshFI GGCC 2 cut(s) 234, 510
BsiSI CCGG 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 445, 517
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 2 cut(s) 234, 510
Bsp13I TCCGGA 1 cut(s) 291
Bsp143I GATC 2 cut(s) 640, 672
Bsp68I TCGCGA 1 cut(s) 219
BspACI CCGC 3 cut(s) 436, 440, 679
BspANI GGCC 2 cut(s) 234, 510
BspEI TCCGGA 1 cut(s) 291
BspFNI CGCG 2 cut(s) 146, 219
BspLI GGNNCC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 442
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 3 cut(s) 183, 283, 537
BssMI GATC 2 cut(s) 640, 672
BssT1I CCWWGG 2 cut(s) 183, 537
Bst2UI CCWGG 2 cut(s) 285, 527
Bst4CI ACNGT 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 150, 217, 232, 440
BstDEI CTNAG 1 cut(s) 587
BstEII GGTNACC 1 cut(s) 545
BstF5I GGATG 2 cut(s) 178, 238
BstFNI CGCG 2 cut(s) 146, 219
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 643, 675
BstMBI GATC 2 cut(s) 640, 672
BstMWI GCNNNNNNNGC 2 cut(s) 216, 656
BstNI CCWGG 2 cut(s) 285, 527
BstPI GGTNACC 1 cut(s) 545
BstSCI CCNGG 2 cut(s) 283, 525
BstUI CGCG 2 cut(s) 146, 219
BstV1I GCAGC 1 cut(s) 723
BstV2I GAAGAC 1 cut(s) 318
BsuRI GGCC 2 cut(s) 234, 510
BtsCI GGATG 2 cut(s) 178, 238
BtuMI TCGCGA 1 cut(s) 219
Cac8I GCNNGC 4 cut(s) 150, 217, 232, 440
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 508, 523
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 3 cut(s) 268, 342, 584
CviAII CATG 4 cut(s) 76, 212, 709, 765
CviQI GTAC 3 cut(s) 268, 342, 584
DdeI CTNAG 1 cut(s) 587
DpnI GATC 2 cut(s) 642, 674
DpnII GATC 2 cut(s) 640, 672
EciI GGCGGA 1 cut(s) 425
Eco130I CCWWGG 2 cut(s) 183, 537
Eco47I GGWCC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 523
EcoO65I GGTNACC 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 283, 525
EcoT14I CCWWGG 2 cut(s) 183, 537
ErhI CCWWGG 2 cut(s) 183, 537
FaeI CATG 4 cut(s) 79, 215, 712, 768
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FatI CATG 4 cut(s) 75, 211, 708, 764
FauI CCCGC 1 cut(s) 447
FauNDI CATATG 2 cut(s) 307, 572
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 2 cut(s) 162, 333
Fnu4HI GCNGC 1 cut(s) 737
FokI GGATG 2 cut(s) 165, 225
Fsp4HI GCNGC 1 cut(s) 737
FspBI CTAG 2 cut(s) 377, 399
GlaI GCGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 737
HaeIII GGCC 2 cut(s) 234, 510
HapII CCGG 1 cut(s) 292
HhaI GCGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 79, 215, 712, 768
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 9 cut(s) 98, 164, 188, 288, 432, 452, 489, 597, 625
HpaII CCGG 1 cut(s) 292
HphI GGTGA 2 cut(s) 127, 557
Hpy166II GTNNAC 4 cut(s) 163, 206, 270, 334
Hpy188I TCNGA 4 cut(s) 193, 596, 630, 640
Hpy188III TCNNGA 4 cut(s) 170, 218, 292, 622
Hpy8I GTNNAC 4 cut(s) 163, 206, 270, 334
Hpy99I CGWCG 2 cut(s) 161, 279
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 415, 465, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 656
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 4 cut(s) 79, 215, 712, 768
HspAI GCGC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 291
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 2 cut(s) 640, 672
LmnI GCTCC 2 cut(s) 72, 294
Lsp1109I GCAGC 1 cut(s) 723
LweI GCATC 4 cut(s) 424, 600, 658, 718
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 2 cut(s) 377, 399
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 5 cut(s) 133, 140, 365, 545, 740
MalI GATC 2 cut(s) 642, 674
MbiI CCGCTC 1 cut(s) 442
MboI GATC 2 cut(s) 640, 672
MboII GAAGA 3 cut(s) 323, 362, 381
MluCI AATT 4 cut(s) 56, 327, 460, 745
MlyI GAGTC 3 cut(s) 107, 158, 446
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 9 cut(s) 10, 45, 233, 245, 254, 442, 514, 598, 765
MroI TCCGGA 1 cut(s) 291
MseI TTAA 1 cut(s) 662
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 2 cut(s) 285, 527
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 2 cut(s) 285, 527
MvnI CGCG 2 cut(s) 146, 219
MwoI GCNNNNNNNGC 2 cut(s) 216, 656
NdeI CATATG 2 cut(s) 307, 572
NdeII GATC 2 cut(s) 640, 672
NlaIII CATG 4 cut(s) 79, 215, 712, 768
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 3 cut(s) 133, 545, 740
NruI TCGCGA 1 cut(s) 219
PctI GAATGC 1 cut(s) 637
PfeI GAWTC 6 cut(s) 188, 288, 432, 489, 597, 625
PkrI GCNGC 1 cut(s) 738
PleI GAGTC 3 cut(s) 106, 158, 446
PpsI GAGTC 3 cut(s) 106, 158, 446
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 283, 525
PspEI GGTNACC 1 cut(s) 545
PspGI CCWGG 2 cut(s) 283, 525
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 2 cut(s) 508, 523
PspPPI RGGWCCY 1 cut(s) 523
RruI TCGCGA 1 cut(s) 219
RsaI GTAC 3 cut(s) 269, 343, 585
RsaNI GTAC 3 cut(s) 268, 342, 584
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 1 cut(s) 737
Sau3AI GATC 2 cut(s) 640, 672
Sau96I GGNCC 2 cut(s) 508, 523
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 3 cut(s) 107, 158, 446
ScrFI CCNGG 2 cut(s) 285, 527
SetI ASST 8 cut(s) 135, 225, 232, 378, 528, 555, 693, 704
SexAI ACCWGGT 1 cut(s) 525
SfaNI GCATC 4 cut(s) 424, 600, 658, 718
SinI GGWCC 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 4 cut(s) 56, 327, 460, 745
SsiI CCGC 3 cut(s) 436, 440, 679
SspMI CTAG 2 cut(s) 377, 399
StyD4I CCNGG 2 cut(s) 283, 525
StyI CCWWGG 2 cut(s) 183, 537
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 101, 333
TasI AATT 4 cut(s) 56, 327, 460, 745
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 6 cut(s) 188, 288, 432, 489, 597, 625
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TseFI GTSAC 3 cut(s) 133, 545, 740
TseI GCWGC 1 cut(s) 736
Tsp45I GTSAC 3 cut(s) 133, 545, 740
TspDTI ATGAA 3 cut(s) 210, 324, 738
TspGWI ACGGA 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 2 cut(s) 162, 333
XspI CTAG 2 cut(s) 377, 399
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.