pycom14g00140

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016574
Physical Location & Seq
Reverse (-)
76348 .. 80002
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00140.1

Sequence Viewer

Length: 825 bp
ATGGTTATGTGTGTTCTTCCGTTGTTGCTGTTGCTGTGCGTTGCAGAGAGAGGAGGGGGGGAGAGAGAGAGAGAGAGAGAGAAGAGAAAGACGGAATTAGGGCTTTTGGGGAGGATGGAGTTGATAGCAGAAAAGGAGTCGAACCTCAATAACAATGAGAACAACAACAACAACAATAACGTCACAAGTTACGCGCAAGCACTCTTAGACTCTCACGATAATCATCCCAACGAATCCGATGAAGTTCGTGAACGCCATGCTCGCGACCTCAAAGCTGGCCTCCATCCTCTCAAGCACAAGTTCGTATTTTGGTACACTCGCCGAACTCCAGGAATCCGGAGCCAGACATCATATGAAGACAATATAAAGAAAATTGTCGACTTCAGTACGGTTGAAGGTTTTTGGGTCAGTTATTGCCACCTAGCCCGACCTTCTTCTTTGCCTAGTCCAACAGACTTGCATCTTTTCAAGGATGGCATTCGCCCTCTCTGGGAGGACTCTGCTAATTGCAACGGTGGCAAATGGATAATACGATTCAAAAAGGCTGTGTCGGGACGTTTTTGGGAGGACCTGGTTCTTGCCTTGGTGGGTGACCAGCTTGATTATGGAGAGAACATATGTGGTGCAGTACTAAGCATCAGATTCAACGAGGATATATTGAGTGTCTGGAACCGGAATGCTTCTGATCATCAGGCTGTAATGGCTTTAAGAGATGCGATCAAGCGGAACTTGAAGCTTCCACACAGCTATCTCATGGAATACAAGCCACATGATGCTTCGCTGCGCGACAATTCATCTTACAGAAACACATGGTTGAGGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko03013 ko04066 ko04150 ko04151 ko04211 ko04910 map01521 map03013 map04066 map04150 map04151 map04211 map04910
Pfam Domains
Protein Families
IF4E

Protein Analysis

275

Amino Acids

31.58

Weight (kDa)

7.71

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 96 - 254 4.9e-55 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016574)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18110
fragaria_vesca FvH4_1g28260
malus_domestica MD12G1004900.v1.1 MD14G1003400.v1.1
prunus_persica Prupe.7G005900_v2.0.a1
pyrus_communis pycom12g00400 pycom14g00140
rosa_chinensis RchiOBHm_Chr3g0496901
rosa_laevigata RLG00000022491
rosa_roxburghii Rroxscaffold_6G00388600
rosa_rugosa Rorug03G0288400
rosa_samantha Rh3AG332300 Rh3DG368300
rosa_wichuraiana Rw3G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 378
AccII CGCG 3 cut(s) 194, 264, 786
AccIII TCCGGA 1 cut(s) 336
AciI CCGC 1 cut(s) 724
AcuI CTGAAG 1 cut(s) 367
AfaI GTAC 3 cut(s) 314, 388, 630
AfiI CCNNNNNNNGG 1 cut(s) 490
AgsI TTSAA 5 cut(s) 395, 469, 538, 646, 733
AjnI CCWGG 2 cut(s) 328, 570
AluBI AGCT 4 cut(s) 275, 598, 736, 747
AluI AGCT 4 cut(s) 275, 598, 736, 747
Aor13HI TCCGGA 1 cut(s) 336
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 781
AspLEI GCGC 2 cut(s) 196, 786
AspS9I GGNCC 1 cut(s) 568
AsuHPI GGTGA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 568
BbsI GAAGAC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 768
BccI CCATC 3 cut(s) 109, 291, 467
BciT130I CCWGG 2 cut(s) 330, 572
BclI TGATCA 1 cut(s) 685
BfaI CTAG 3 cut(s) 422, 444, 823
BisI GCNGC 1 cut(s) 782
BlsI GCNGC 1 cut(s) 783
BmcAI AGTACT 1 cut(s) 630
Bme1390I CCNGG 2 cut(s) 330, 572
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 2 cut(s) 341, 671
BmrFI CCNGG 2 cut(s) 330, 572
BmsI GCATC 4 cut(s) 469, 645, 703, 763
BpiI GAAGAC 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 582
BsaWI WCCGGW 2 cut(s) 336, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 336
BseBI CCWGG 2 cut(s) 330, 572
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 4 cut(s) 120, 223, 283, 478
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 768
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 3 cut(s) 194, 264, 786
BshFI GGCC 1 cut(s) 279
BsiSI CCGG 2 cut(s) 337, 673
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 567
BsmI GAATGC 2 cut(s) 477, 682
BsnI GGCC 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 336
Bsp143I GATC 2 cut(s) 685, 717
Bsp68I TCGCGA 1 cut(s) 264
BspACI CCGC 1 cut(s) 724
BspANI GGCC 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 336
BspFNI CGCG 3 cut(s) 194, 264, 786
BspLI GGNNCC 2 cut(s) 341, 671
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 2 cut(s) 685, 717
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 2 cut(s) 330, 572
Bst4CI ACNGT 2 cut(s) 391, 515
Bst6I CTCTTC 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 198, 262, 277
BstDEI CTNAG 2 cut(s) 205, 632
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 4 cut(s) 120, 223, 283, 478
BstFNI CGCG 3 cut(s) 194, 264, 786
BstHHI GCGC 2 cut(s) 196, 786
BstKTI GATC 2 cut(s) 688, 720
BstMBI GATC 2 cut(s) 685, 717
BstMWI GCNNNNNNNGC 3 cut(s) 261, 516, 701
BstNI CCWGG 2 cut(s) 330, 572
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 328, 570
BstUI CGCG 3 cut(s) 194, 264, 786
BstV1I GCAGC 1 cut(s) 768
BstV2I GAAGAC 1 cut(s) 363
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 4 cut(s) 120, 223, 283, 478
BtuMI TCGCGA 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 198, 262, 277
CfoI GCGC 2 cut(s) 196, 786
Cfr13I GGNCC 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 3 cut(s) 313, 387, 629
CviAII CATG 4 cut(s) 257, 754, 770, 810
CviQI GTAC 3 cut(s) 313, 387, 629
DdeI CTNAG 2 cut(s) 205, 632
DpnI GATC 2 cut(s) 687, 719
DpnII GATC 2 cut(s) 685, 717
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 328, 570
EcoT14I CCWWGG 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 4 cut(s) 260, 757, 773, 813
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 1 cut(s) 567
FatI CATG 4 cut(s) 256, 753, 769, 809
FauNDI CATATG 2 cut(s) 352, 617
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 782
FokI GGATG 4 cut(s) 127, 210, 270, 485
Fsp4HI GCNGC 1 cut(s) 782
FspBI CTAG 3 cut(s) 422, 444, 823
GlaI GCGC 2 cut(s) 195, 785
GluI GCNGC 1 cut(s) 782
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 1 cut(s) 279
HapII CCGG 2 cut(s) 337, 673
HhaI GCGC 2 cut(s) 196, 786
Hin1II CATG 4 cut(s) 260, 757, 773, 813
Hin6I GCGC 2 cut(s) 194, 784
HinP1I GCGC 2 cut(s) 194, 784
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 7 cut(s) 137, 209, 233, 333, 497, 534, 642
HpaII CCGG 2 cut(s) 337, 673
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 3 cut(s) 251, 315, 379
Hpy188I TCNGA 3 cut(s) 238, 641, 685
Hpy188III TCNNGA 6 cut(s) 215, 248, 263, 337, 552, 667
Hpy8I GTNNAC 3 cut(s) 251, 315, 379
HpyAV CCTTC 2 cut(s) 389, 441
HpyCH4III ACNGT 2 cut(s) 391, 515
HpyCH4IV ACGT 2 cut(s) 180, 556
HpyCH4V TGCA 4 cut(s) 44, 460, 510, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 516, 701
HpyF3I CTNAG 2 cut(s) 205, 632
HpySE526I ACGT 2 cut(s) 180, 556
Hsp92II CATG 4 cut(s) 260, 757, 773, 813
HspAI GCGC 2 cut(s) 194, 784
Kpn2I TCCGGA 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 2 cut(s) 685, 717
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 1 cut(s) 768
LweI GCATC 4 cut(s) 469, 645, 703, 763
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 3 cut(s) 422, 444, 823
MaeII ACGT 2 cut(s) 180, 556
MaeIII GTNAC 3 cut(s) 181, 188, 590
MalI GATC 2 cut(s) 687, 719
MboI GATC 2 cut(s) 685, 717
MboII GAAGA 4 cut(s) 8, 94, 368, 426
MluCI AATT 4 cut(s) 95, 372, 505, 790
MlyI GAGTC 3 cut(s) 146, 203, 491
MmeI TCCRAC 1 cut(s) 473
MroI TCCGGA 1 cut(s) 336
MseI TTAA 1 cut(s) 707
MspI CCGG 2 cut(s) 337, 673
MspR9I CCNGG 2 cut(s) 330, 572
Mva1269I GAATGC 2 cut(s) 477, 682
MvaI CCWGG 2 cut(s) 330, 572
MvnI CGCG 3 cut(s) 194, 264, 786
MwoI GCNNNNNNNGC 3 cut(s) 261, 516, 701
NdeI CATATG 2 cut(s) 352, 617
NdeII GATC 2 cut(s) 685, 717
NlaIII CATG 4 cut(s) 260, 757, 773, 813
NlaIV GGNNCC 2 cut(s) 341, 671
NmuCI GTSAC 2 cut(s) 181, 590
NruI TCGCGA 1 cut(s) 264
PctI GAATGC 2 cut(s) 477, 682
PfeI GAWTC 4 cut(s) 233, 333, 534, 642
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 1 cut(s) 783
PleI GAGTC 3 cut(s) 145, 203, 491
PpsI GAGTC 3 cut(s) 145, 203, 491
PpuMI RGGWCCY 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 328, 570
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 2 cut(s) 328, 570
PspN4I GGNNCC 2 cut(s) 341, 671
PspPI GGNCC 1 cut(s) 568
PspPPI RGGWCCY 1 cut(s) 568
RruI TCGCGA 1 cut(s) 264
RsaI GTAC 3 cut(s) 314, 388, 630
RsaNI GTAC 3 cut(s) 313, 387, 629
SalI GTCGAC 1 cut(s) 377
SaqAI TTAA 1 cut(s) 707
SatI GCNGC 1 cut(s) 782
Sau3AI GATC 2 cut(s) 685, 717
Sau96I GGNCC 1 cut(s) 568
ScaI AGTACT 1 cut(s) 630
SchI GAGTC 3 cut(s) 146, 203, 491
ScrFI CCNGG 2 cut(s) 330, 572
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 4 cut(s) 469, 645, 703, 763
SinI GGWCC 1 cut(s) 568
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 95, 372, 505, 790
SsiI CCGC 1 cut(s) 724
SspMI CTAG 3 cut(s) 422, 444, 823
StyD4I CCNGG 2 cut(s) 328, 570
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 2 cut(s) 391, 515
TaiI ACGT 2 cut(s) 183, 559
TaqI TCGA 2 cut(s) 140, 378
TasI AATT 4 cut(s) 95, 372, 505, 790
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 4 cut(s) 233, 333, 534, 642
Tru1I TTAA 1 cut(s) 707
Tru9I TTAA 1 cut(s) 707
TseFI GTSAC 2 cut(s) 181, 590
TseI GCWGC 1 cut(s) 781
Tsp45I GTSAC 2 cut(s) 181, 590
TspDTI ATGAA 3 cut(s) 255, 369, 783
TspGWI ACGGA 2 cut(s) 9, 107
VpaK11BI GGWCC 1 cut(s) 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 378
XspI CTAG 3 cut(s) 422, 444, 823
ZrmI AGTACT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.