MD12G1024300.v1.1
BZIP Family

Protein ABSCISIC ACID-INSENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006875
Physical Location & Seq
Forward (+)
2461297 .. 2463189
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024300.v1.1.491

Sequence Viewer

Length: 708 bp
ATGACATTGGAGGATTTTTTGGTTAAAGCAGGGGTAGTTCGGGAACAGGAATCAATGGCAGCCACAGTGGTGCCTCCTCAGCCTCAGCAGCAGCAGCAATATGGGATGTATCAGAACGGCAACCAGGCAGTGGTACCGAGTTTTGTTAATAGGCCTGTGATGGGAATGGGGGCTGCTGGTGCAGCAGGTACTAGCACTGCTACCGGTATTCGGAATTACCAAACTATACCACAAAGTGGGTCTGCGGTTGTGGGAGAGTCCTCTGCGTATGCTGCGAATGGTAAGAGGAATGGGGCTTACCCGACAGTGCCACCTCCACAGTCGGTTTGTTTTGGCGGGAGAGTGGTGAATGGTGGTGATGGATATGCAGCAGGGCAGACAATTGGGATGGGGGCTCTTGTGAGTCCGGTGTCTTCTGATGGGATGGGTACTAGTCAGGTTGAGAACTCTGGGGGTCAATTTGGTTTGGAAATGGGTGGACTAAGAGGAAGGAAGCGGGGTTTAGATGGAGCGGTTGAAAAAGTGGTAGAGAGGAGGCAGAGAAGGATGATTAAGAACAGAGAGTCTGCAGCAAGGTCTAGAGCCCGAAAACAGGCATACACAGTTGAACTGGAAGCAGAACTGAATCAATTGCGAGAAGAGAACTCACACCTTAATCAGGCGCTGAGCTCGAGAGGAAACGAAAGCAACAGTATTTCGAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010152 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0080090 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

236

Amino Acids

24.84

Weight (kDa)

9.75

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bZIP_2 PF07716 177 - 224 1.4e-09 Basic region leucine zipper
bZIP_1 PF00170 178 - 225 3.2e-12 bZIP transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 2 cut(s) 70, 133
AccB7I CCANNNNNTGG 2 cut(s) 130, 236
AccBSI CCGCTC 1 cut(s) 512
AciI CCGC 4 cut(s) 245, 336, 496, 512
AdeI CACNNNGTG 1 cut(s) 236
AfaI GTAC 3 cut(s) 135, 190, 430
AfiI CCNNNNNNNGG 6 cut(s) 130, 161, 210, 236, 592, 658
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 2 cut(s) 518, 608
AhlI ACTAGT 1 cut(s) 431
AjnI CCWGG 1 cut(s) 123
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 1 cut(s) 669
AluI AGCT 1 cut(s) 669
Alw21I GWGCWC 1 cut(s) 671
AlwNI CAGNNNCTG 1 cut(s) 664
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 1 cut(s) 152
ApeKI GCWGC 9 cut(s) 59, 88, 91, 94, 173, 182, 272, 368, 569
AsiGI ACCGGT 1 cut(s) 203
Asp718I GGTACC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 664
AsuHPI GGTGA 2 cut(s) 358, 368
AvaI CYCGRG 1 cut(s) 670
BanI GGYRCC 2 cut(s) 70, 133
BanII GRGCYC 3 cut(s) 397, 586, 671
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 671
BbvCI CCTCAGC 2 cut(s) 78, 84
BbvI GCAGC 9 cut(s) 71, 100, 103, 106, 160, 194, 259, 380, 581
BccI CCATC 6 cut(s) 154, 353, 382, 413, 418, 500
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 125
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 3 cut(s) 192, 432, 579
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 9 cut(s) 60, 89, 92, 95, 174, 183, 273, 369, 570
BlpI GCTNAGC 1 cut(s) 665
BlsI GCNGC 9 cut(s) 61, 90, 93, 96, 175, 184, 274, 370, 571
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 670
BmiI GGNNCC 2 cut(s) 72, 135
BmrFI CCNGG 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 405
Bpu10I CCTNAGC 2 cut(s) 78, 84
Bpu1102I GCTNAGC 1 cut(s) 665
BsaWI WCCGGW 2 cut(s) 203, 406
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 161, 210, 236, 592, 658
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 125
BseGI GGATG 4 cut(s) 111, 393, 429, 552
BseLI CCNNNNNNNGG 6 cut(s) 130, 161, 210, 236, 592, 658
BseMII CTCAG 3 cut(s) 92, 98, 656
BseNI ACTGG 1 cut(s) 615
BseRI GAGGAG 2 cut(s) 66, 547
BseXI GCAGC 9 cut(s) 71, 100, 103, 106, 160, 194, 259, 380, 581
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 1 cut(s) 154
BshNI GGYRCC 2 cut(s) 70, 133
BshTI ACCGGT 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 671
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 2 cut(s) 204, 407
BslI CCNNNNNNNGG 6 cut(s) 130, 161, 210, 236, 592, 658
BsnI GGCC 1 cut(s) 154
BsoBI CYCGRG 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 397, 586, 671
Bsp1720I GCTNAGC 1 cut(s) 665
BspACI CCGC 4 cut(s) 245, 336, 496, 512
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 3 cut(s) 91, 97, 657
BspLI GGNNCC 2 cut(s) 72, 135
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 176
BspT107I GGYRCC 2 cut(s) 70, 133
BsrBI CCGCTC 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 615
BssAI RCCGGY 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 5 cut(s) 67, 307, 321, 604, 692
Bst6I CTCTTC 1 cut(s) 633
BstDEI CTNAG 4 cut(s) 78, 84, 482, 665
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 4 cut(s) 111, 393, 429, 552
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 1 cut(s) 664
BstMWI GCNNNNNNNGC 6 cut(s) 79, 88, 94, 179, 182, 272
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 9 cut(s) 71, 100, 103, 106, 160, 194, 259, 380, 581
BstV2I GAAGAC 1 cut(s) 405
BsuRI GGCC 1 cut(s) 154
BtsCI GGATG 4 cut(s) 111, 393, 429, 552
BtsI GCAGTG 2 cut(s) 135, 195
BtsIMutI CAGTG 4 cut(s) 72, 135, 195, 312
BveI ACCTGC 1 cut(s) 176
CaiI CAGNNNCTG 1 cut(s) 664
CfoI GCGC 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 203
Csp6I GTAC 3 cut(s) 134, 189, 429
CspAI ACCGGT 1 cut(s) 203
CviJI RGCY 8 cut(s) 62, 82, 154, 173, 296, 395, 584, 669
CviKI_1 RGCY 8 cut(s) 62, 82, 154, 173, 296, 395, 584, 669
CviQI GTAC 3 cut(s) 134, 189, 429
DdeI CTNAG 4 cut(s) 78, 84, 482, 665
DraIII CACNNNGTG 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 669
Eco147I AGGCCT 1 cut(s) 154
Eco24I GRGCYC 3 cut(s) 397, 586, 671
Eco53kI GAGCTC 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 670
EcoICRI GAGCTC 1 cut(s) 669
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoRII CCWGG 1 cut(s) 123
EcoT38I GRGCYC 3 cut(s) 397, 586, 671
FaiI YATR 5 cut(s) 102, 227, 270, 366, 598
FauI CCCGC 2 cut(s) 329, 489
Fnu4HI GCNGC 9 cut(s) 60, 89, 92, 95, 174, 183, 273, 369, 570
FokI GGATG 4 cut(s) 118, 400, 436, 559
FriOI GRGCYC 3 cut(s) 397, 586, 671
Fsp4HI GCNGC 9 cut(s) 60, 89, 92, 95, 174, 183, 273, 369, 570
FspBI CTAG 3 cut(s) 192, 432, 579
GlaI GCGC 1 cut(s) 663
GluI GCNGC 9 cut(s) 60, 89, 92, 95, 174, 183, 273, 369, 570
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 1 cut(s) 154
HapII CCGG 2 cut(s) 204, 407
HhaI GCGC 1 cut(s) 664
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HinfI GANTC 5 cut(s) 50, 257, 403, 563, 625
HpaII CCGG 2 cut(s) 204, 407
HphI GGTGA 2 cut(s) 358, 368
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 3 cut(s) 114, 213, 418
Hpy188III TCNNGA 3 cut(s) 41, 579, 672
Hpy8I GTNNAC 1 cut(s) 479
HpyAV CCTTC 2 cut(s) 483, 537
HpyCH4III ACNGT 5 cut(s) 67, 307, 321, 604, 692
HpyCH4V TGCA 3 cut(s) 182, 368, 569
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 88, 94, 179, 182, 272
HpyF3I CTNAG 4 cut(s) 78, 84, 482, 665
HspAI GCGC 1 cut(s) 662
KpnI GGTACC 1 cut(s) 137
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 9 cut(s) 71, 100, 103, 106, 160, 194, 259, 380, 581
MaeI CTAG 3 cut(s) 192, 432, 579
MbiI CCGCTC 1 cut(s) 512
MboII GAAGA 2 cut(s) 405, 650
MfeI CAATTG 2 cut(s) 381, 629
MhlI GDGCHC 3 cut(s) 397, 586, 671
MluCI AATT 4 cut(s) 214, 381, 458, 629
MlyI GAGTC 3 cut(s) 266, 412, 572
MseI TTAA 4 cut(s) 24, 147, 552, 654
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 2 cut(s) 204, 407
MspR9I CCNGG 1 cut(s) 125
MunI CAATTG 2 cut(s) 381, 629
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 6 cut(s) 79, 88, 94, 179, 182, 272
NlaIV GGNNCC 2 cut(s) 72, 135
OliI CACNNNNGTG 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 670
PceI AGGCCT 1 cut(s) 154
PfeI GAWTC 2 cut(s) 50, 625
PflMI CCANNNNNTGG 2 cut(s) 130, 236
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 9 cut(s) 61, 90, 93, 96, 175, 184, 274, 370, 571
PleI GAGTC 3 cut(s) 265, 411, 571
PpsI GAGTC 3 cut(s) 265, 411, 571
Psp124BI GAGCTC 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 72, 135
PstI CTGCAG 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 664
RsaI GTAC 3 cut(s) 135, 190, 430
RsaNI GTAC 3 cut(s) 134, 189, 429
RseI CAYNNNNRTG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 671
SaqAI TTAA 4 cut(s) 24, 147, 552, 654
SatI GCNGC 9 cut(s) 60, 89, 92, 95, 174, 183, 273, 369, 570
SchI GAGTC 3 cut(s) 266, 412, 572
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 3 cut(s) 397, 586, 671
SetI ASST 6 cut(s) 190, 316, 441, 578, 654, 671
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 4 cut(s) 214, 381, 458, 629
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 4 cut(s) 245, 336, 496, 512
SspMI CTAG 3 cut(s) 192, 432, 579
SstI GAGCTC 1 cut(s) 671
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 5 cut(s) 67, 307, 321, 604, 692
TaqI TCGA 2 cut(s) 671, 698
TasI AATT 4 cut(s) 214, 381, 458, 629
TfiI GAWTC 2 cut(s) 50, 625
Tru1I TTAA 4 cut(s) 24, 147, 552, 654
Tru9I TTAA 4 cut(s) 24, 147, 552, 654
TscAI CASTG 4 cut(s) 72, 135, 202, 312
TseI GCWGC 9 cut(s) 59, 88, 91, 94, 173, 182, 272, 368, 569
TspRI CASTG 4 cut(s) 72, 135, 202, 312
Van91I CCANNNNNTGG 2 cut(s) 130, 236
XagI CCTNNNNNAGG 1 cut(s) 656
XbaI TCTAGA 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 670
XspI CTAG 3 cut(s) 192, 432, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.