MD14G1021600.v1.1
BZIP Family

Protein ABSCISIC ACID-INSENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006875
Physical Location & Seq
Forward (+)
2018493 .. 2021107
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1021600.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGGTGTTTTGGAGTCGGAAATCAACTCTCATGGCGAGGTGGATTCGCCCTTGTTATCGGACCAACAAGCGAAGAACCACTTATTCTCCTCATTAGGAAGACAATCATCAATCTACTCGCTCACTCTTGATGAGTTTCAGCACACACTCTGCGAGAGCGGCAAGAATTTCGGGTTGATGAATATGGATGAGTTCCTAACCAGCATTTGGACCGCCGAAGAAAACCAAGCCATTAATTCCAATCTCACCAACACTACTACTACTAACAACAACAACAACATGAACAACATTGAGGTGCACATACCTTTAGCCGAGGCCTCAGCAGAAAAGAGCATTGCGACGCAGCCCAGCTTGCCGCGTCAAGGCTCGCTTACGCTGCCTGAGCCACTGTGTAGGAAAACAGTGGATGAAGTTTGGTCTGAGATAAACGGAGGGCAGCAGGCAAAGCAGCAGAACAATCACAACAGTAGCATCGATGGTAGTGTTCAGAGTTCTGAGTTTGCCCCTCGCCAGCCTACTTTTGGGGAGATGACATTGGAGGATTTTTTGGTTAAAGCAGGGGTAGTTCGGGAACAGGATTCAATGGCAGCCACGGTGGTCCCTCCTCAGCCTCACCAGCAGCAGCGGTATGGGATGTACCAGAACGGCAACCATGCTGTGGGACCCAGTTTTGTTAATAGGCCTGTGATGGGAATGGGGGCTGCTGCCGCAGCAGGTGCTAGTACTAGCACTGCCGCCGGTATTCCTAATTACCAAACTATACCGCAAAGTGGGGCTGCGGTTGTGGGAGAGTCCTCTGCGTATGCTGCGAATGGTAAGAGGAATGGGGCGTACCCGGGCGTGCGGCCTCCACAGTCGGTTTGTTTTGGCGGGAGAGTGGAGAATGGTGGTGGTGGATATGCAGCAGGGCAGACAATTGGGATGGCGGCTCCGGGGAGTCCGGTGTCTTCAGATGGGATGGGTACTAGTCAGGTTGAAAACTCTGCGGGTAGATTTGGTTTGGACATGGGTTTAGATGGACTAAGAGGAAGGAAACGGGGTTTAGATGGACCGGTCGAAAAAGTGGTGGAGAGGAGGCAGAGAAGGATGATTAAGAACAGAGAGTCTGCAGCAAGGTCTAGAGCTCGAAAACAGGCATACACAGTTGAATTGGAAGCAGAACTGAACCACTTACGAGAAGAGAACTCACACCTTAAACAGGCGCTGAGCTCGAGAGGAAGCGAAAGCAACAGTATTTCGAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010152 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0080090 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

416

Amino Acids

44.45

Weight (kDa)

6.18

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bZIP_2 PF07716 357 - 403 3.7e-10 Basic region leucine zipper
bZIP_1 PF00170 358 - 404 3.3e-12 bZIP transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 704
Acc36I ACCTGC 1 cut(s) 704
AccB7I CCANNNNNTGG 2 cut(s) 207, 658
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 1 cut(s) 358
AcsI RAATTY 1 cut(s) 166
AcuI CTGAAG 1 cut(s) 933
AfaI GTAC 4 cut(s) 638, 724, 833, 964
AfiI CCNNNNNNNGG 7 cut(s) 207, 362, 521, 658, 689, 770, 1198
AgeI ACCGGT 1 cut(s) 1051
AgsI TTSAA 3 cut(s) 582, 977, 1148
AhlI ACTAGT 1 cut(s) 965
AluBI AGCT 3 cut(s) 351, 1124, 1209
AluI AGCT 3 cut(s) 351, 1124, 1209
Alw21I GWGCWC 3 cut(s) 300, 1126, 1211
Alw44I GTGCAC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 1204
Ama87I CYCGRG 2 cut(s) 835, 1210
AoxI GGCC 3 cut(s) 315, 680, 845
ApaLI GTGCAC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 166
AseI ATTAAT 1 cut(s) 234
AsiGI ACCGGT 1 cut(s) 1051
AspLEI GCGC 1 cut(s) 1204
AspS9I GGNCC 5 cut(s) 61, 210, 598, 662, 1049
AsuC2I CCSGG 3 cut(s) 836, 837, 933
AsuHPI GGTGA 2 cut(s) 238, 605
AvaI CYCGRG 2 cut(s) 835, 1210
AvaII GGWCC 5 cut(s) 61, 210, 598, 662, 1049
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 2 cut(s) 1126, 1211
BbsI GAAGAC 2 cut(s) 106, 939
Bbv12I GWGCWC 3 cut(s) 300, 1126, 1211
BbvCI CCTCAGC 2 cut(s) 319, 606
BccI CCATC 7 cut(s) 470, 682, 916, 947, 952, 1010, 1040
BceAI ACGGC 1 cut(s) 661
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BcnI CCSGG 3 cut(s) 836, 837, 933
BcuI ACTAGT 1 cut(s) 965
BfaI CTAG 4 cut(s) 720, 726, 966, 1119
BfmI CTRYAG 1 cut(s) 1107
BfoI RGCGCY 1 cut(s) 1205
BfuAI ACCTGC 1 cut(s) 704
BlpI GCTNAGC 1 cut(s) 1205
BmcAI AGTACT 1 cut(s) 724
Bme1390I CCNGG 3 cut(s) 836, 837, 933
Bme18I GGWCC 5 cut(s) 61, 210, 598, 662, 1049
BmeT110I CYCGRG 2 cut(s) 835, 1210
BmgT120I GGNCC 5 cut(s) 61, 210, 598, 662, 1049
BmiI GGNNCC 4 cut(s) 600, 663, 664, 930
BmrFI CCNGG 3 cut(s) 836, 837, 933
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 480
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 106, 939
Bpu10I CCTNAGC 3 cut(s) 319, 381, 606
Bpu1102I GCTNAGC 1 cut(s) 1205
BpuMI CCSGG 3 cut(s) 836, 837, 933
Bsa29I ATCGAT 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 312, 591, 835, 932
BsaWI WCCGGW 2 cut(s) 940, 1051
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 7 cut(s) 207, 362, 521, 658, 689, 770, 1198
Bse118I RCCGGY 2 cut(s) 737, 1051
Bse1I ACTGG 1 cut(s) 666
Bse3DI GCAATG 1 cut(s) 333
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 4 cut(s) 312, 591, 835, 932
BseGI GGATG 6 cut(s) 193, 412, 639, 927, 963, 1092
BseLI CCNNNNNNNGG 7 cut(s) 207, 362, 521, 658, 689, 770, 1198
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 6 cut(s) 333, 372, 411, 486, 620, 1196
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 3 cut(s) 79, 594, 1087
BseSI GKGCMC 1 cut(s) 300
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 358
Bsh1285I CGRYCG 1 cut(s) 1056
BshFI GGCC 3 cut(s) 317, 682, 847
BshTI ACCGGT 1 cut(s) 1051
BshVI ATCGAT 1 cut(s) 474
BsiEI CGRYCG 1 cut(s) 1056
BsiHKAI GWGCWC 3 cut(s) 300, 1126, 1211
BsiHKCI CYCGRG 2 cut(s) 835, 1210
BsiSI CCGG 5 cut(s) 738, 836, 932, 941, 1052
BslFI GGGAC 2 cut(s) 584, 675
BslI CCNNNNNNNGG 7 cut(s) 207, 362, 521, 658, 689, 770, 1198
BsmFI GGGAC 2 cut(s) 584, 675
BsnI GGCC 3 cut(s) 317, 682, 847
BsoBI CYCGRG 2 cut(s) 835, 1210
Bsp1286I GDGCHC 3 cut(s) 300, 1126, 1211
Bsp1720I GCTNAGC 1 cut(s) 1205
BspANI GGCC 3 cut(s) 317, 682, 847
BspCNI CTCAG 6 cut(s) 332, 373, 412, 487, 619, 1197
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 1 cut(s) 358
BspLI GGNNCC 4 cut(s) 600, 663, 664, 930
BspMAI CTGCAG 1 cut(s) 1111
BspMI ACCTGC 1 cut(s) 704
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 2 cut(s) 737, 1051
BsrI ACTGG 1 cut(s) 666
BssAI RCCGGY 2 cut(s) 737, 1051
BssECI CCNNGG 4 cut(s) 312, 591, 835, 932
Bst4CI ACNGT 7 cut(s) 390, 403, 467, 595, 855, 1144, 1232
Bst6I CTCTTC 1 cut(s) 1173
BstAPI GCANNNNNTGC 1 cut(s) 716
BstC8I GCNNGC 5 cut(s) 353, 368, 441, 512, 842
BstDEI CTNAG 7 cut(s) 319, 381, 420, 495, 606, 1022, 1205
BstDSI CCRYGG 1 cut(s) 591
BstENI CCTNNNNNAGG 1 cut(s) 1196
BstF5I GGATG 6 cut(s) 193, 412, 639, 927, 963, 1092
BstFNI CGCG 1 cut(s) 358
BstH2I RGCGCY 1 cut(s) 1205
BstHHI GCGC 1 cut(s) 1204
BstMCI CGRYCG 1 cut(s) 1056
BstSCI CCNGG 3 cut(s) 834, 835, 931
BstSFI CTRYAG 1 cut(s) 1107
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 1 cut(s) 358
BstV2I GAAGAC 2 cut(s) 106, 939
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 3 cut(s) 317, 682, 847
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 6 cut(s) 193, 412, 639, 927, 963, 1092
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 3 cut(s) 386, 408, 729
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 5 cut(s) 353, 368, 441, 512, 842
CaiI CAGNNNCTG 1 cut(s) 1204
CfoI GCGC 1 cut(s) 1204
Cfr10I RCCGGY 2 cut(s) 737, 1051
Cfr13I GGNCC 5 cut(s) 61, 210, 598, 662, 1049
Cfr9I CCCGGG 1 cut(s) 835
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 2 cut(s) 347, 349
Csp6I GTAC 4 cut(s) 637, 723, 832, 963
CspAI ACCGGT 1 cut(s) 1051
CviAII CATG 4 cut(s) 32, 280, 653, 1006
CviQI GTAC 4 cut(s) 637, 723, 832, 963
DdeI CTNAG 7 cut(s) 319, 381, 420, 495, 606, 1022, 1205
Eam1104I CTCTTC 1 cut(s) 1173
EarI CTCTTC 1 cut(s) 1173
Ecl136II GAGCTC 2 cut(s) 1124, 1209
Eco147I AGGCCT 2 cut(s) 317, 682
Eco24I GRGCYC 2 cut(s) 1126, 1211
Eco47I GGWCC 5 cut(s) 61, 210, 598, 662, 1049
Eco53kI GAGCTC 2 cut(s) 1124, 1209
Eco57I CTGAAG 1 cut(s) 933
Eco88I CYCGRG 2 cut(s) 835, 1210
EcoICRI GAGCTC 2 cut(s) 1124, 1209
EcoNI CCTNNNNNAGG 1 cut(s) 1196
EcoO109I RGGNCCY 1 cut(s) 662
EcoT38I GRGCYC 2 cut(s) 1126, 1211
FaeI CATG 4 cut(s) 35, 283, 656, 1009
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 354, 386
FaqI GGGAC 2 cut(s) 584, 675
FatI CATG 4 cut(s) 31, 279, 652, 1005
FauI CCCGC 2 cut(s) 863, 979
FokI GGATG 6 cut(s) 200, 419, 646, 934, 970, 1099
FriOI GRGCYC 2 cut(s) 1126, 1211
FspBI CTAG 4 cut(s) 720, 726, 966, 1119
GlaI GCGC 1 cut(s) 1203
GsaI CCCAGC 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 1205
HaeIII GGCC 3 cut(s) 317, 682, 847
HapII CCGG 5 cut(s) 738, 836, 932, 941, 1052
HgaI GACGC 2 cut(s) 347, 349
HhaI GCGC 1 cut(s) 1204
Hin1II CATG 4 cut(s) 35, 283, 656, 1009
Hin6I GCGC 1 cut(s) 1202
HinP1I GCGC 1 cut(s) 1202
HinfI GANTC 6 cut(s) 14, 44, 578, 791, 937, 1103
HpaII CCGG 5 cut(s) 738, 836, 932, 941, 1052
HphI GGTGA 2 cut(s) 238, 605
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 6 cut(s) 19, 61, 421, 489, 496, 952
Hpy188III TCNNGA 4 cut(s) 128, 569, 1119, 1212
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 1023, 1077
HpyCH4III ACNGT 7 cut(s) 390, 403, 467, 595, 855, 1144, 1232
HpyCH4V TGCA 3 cut(s) 298, 902, 1109
HpyF3I CTNAG 7 cut(s) 319, 381, 420, 495, 606, 1022, 1205
Hsp92II CATG 4 cut(s) 35, 283, 656, 1009
HspAI GCGC 1 cut(s) 1202
KflI GGGWCCC 1 cut(s) 662
LmnI GCTCC 1 cut(s) 934
LweI GCATC 1 cut(s) 480
MaeI CTAG 4 cut(s) 720, 726, 966, 1119
MbiI CCGCTC 1 cut(s) 159
MboII GAAGA 5 cut(s) 85, 111, 230, 939, 1190
MfeI CAATTG 1 cut(s) 915
MhlI GDGCHC 3 cut(s) 300, 1126, 1211
MluCI AATT 5 cut(s) 166, 235, 748, 915, 1148
MlyI GAGTC 4 cut(s) 23, 800, 946, 1112
MseI TTAA 5 cut(s) 234, 552, 675, 1092, 1194
MslI CAYNNNNRTG 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 5 cut(s) 738, 836, 932, 941, 1052
MspR9I CCNGG 3 cut(s) 836, 837, 933
MunI CAATTG 1 cut(s) 915
MvnI CGCG 1 cut(s) 358
NciI CCSGG 3 cut(s) 836, 837, 933
NlaIII CATG 4 cut(s) 35, 283, 656, 1009
NlaIV GGNNCC 4 cut(s) 600, 663, 664, 930
NmeAIII GCCGAG 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 1210
PaqCI CACCTGC 1 cut(s) 704
PceI AGGCCT 2 cut(s) 317, 682
PfeI GAWTC 2 cut(s) 44, 578
PflMI CCANNNNNTGG 2 cut(s) 207, 658
PinAI ACCGGT 1 cut(s) 1051
PleI GAGTC 4 cut(s) 22, 799, 945, 1111
PpsI GAGTC 4 cut(s) 22, 799, 945, 1111
PpuMI RGGWCCY 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 234
Psp124BI GAGCTC 2 cut(s) 1126, 1211
Psp5II RGGWCCY 1 cut(s) 662
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 4 cut(s) 600, 663, 664, 930
PspPI GGNCC 5 cut(s) 61, 210, 598, 662, 1049
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 1 cut(s) 1111
PstNI CAGNNNCTG 1 cut(s) 1204
RsaI GTAC 4 cut(s) 638, 724, 833, 964
RsaNI GTAC 4 cut(s) 637, 723, 832, 963
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 2 cut(s) 1126, 1211
SaqAI TTAA 5 cut(s) 234, 552, 675, 1092, 1194
Sau96I GGNCC 5 cut(s) 61, 210, 598, 662, 1049
ScaI AGTACT 1 cut(s) 724
SchI GAGTC 4 cut(s) 23, 800, 946, 1112
ScrFI CCNGG 3 cut(s) 836, 837, 933
SduI GDGCHC 3 cut(s) 300, 1126, 1211
SfaNI GCATC 1 cut(s) 480
SfcI CTRYAG 1 cut(s) 1107
Sfr274I CTCGAG 1 cut(s) 1210
SinI GGWCC 5 cut(s) 61, 210, 598, 662, 1049
SlaI CTCGAG 1 cut(s) 1210
SmaI CCCGGG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 1210
SmoI CTYRAG 1 cut(s) 1210
SpeI ACTAGT 1 cut(s) 965
Sse9I AATT 5 cut(s) 166, 235, 748, 915, 1148
SseBI AGGCCT 2 cut(s) 317, 682
SspMI CTAG 4 cut(s) 720, 726, 966, 1119
SstI GAGCTC 2 cut(s) 1126, 1211
StuI AGGCCT 2 cut(s) 317, 682
StyD4I CCNGG 3 cut(s) 834, 835, 931
TaaI ACNGT 7 cut(s) 390, 403, 467, 595, 855, 1144, 1232
TaqI TCGA 5 cut(s) 474, 1056, 1126, 1211, 1238
TasI AATT 5 cut(s) 166, 235, 748, 915, 1148
TatI WGTACW 1 cut(s) 722
TauI GCSGC 6 cut(s) 162, 358, 710, 737, 847, 929
TfiI GAWTC 2 cut(s) 44, 578
Tru1I TTAA 5 cut(s) 234, 552, 675, 1092, 1194
Tru9I TTAA 5 cut(s) 234, 552, 675, 1092, 1194
TscAI CASTG 3 cut(s) 393, 408, 736
TspDTI ATGAA 3 cut(s) 194, 296, 423
TspGWI ACGGA 1 cut(s) 444
TspMI CCCGGG 1 cut(s) 835
TspRI CASTG 3 cut(s) 393, 408, 736
Van91I CCANNNNNTGG 2 cut(s) 207, 658
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 5 cut(s) 61, 210, 598, 662, 1049
VspI ATTAAT 1 cut(s) 234
XagI CCTNNNNNAGG 1 cut(s) 1196
XapI RAATTY 1 cut(s) 166
XbaI TCTAGA 1 cut(s) 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 1210
XmaI CCCGGG 1 cut(s) 835
XspI CTAG 4 cut(s) 720, 726, 966, 1119
ZrmI AGTACT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.