MD12G1034900.v1.1
BZIP Family

Protein ABSCISIC ACID-INSENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006875
Physical Location & Seq
Forward (+)
3819675 .. 3822415
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1034900.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGTGCTTCAATTTGATCGGGTTCATTACAATTTTGAAACCCATCATTCAATCAGGTGTAGATCACACAAACATGGGTGTTTCGGAGTCGGAAATCATCTCCCAAGGCAAGGTGGATTCACCTTTGTTATCGGACCAACAAGAGAAGAACCAGTTATTCTCTTCATTAGGAAGACAATCATCAATCTACTCTCTCACTCTTGATGAGTTTCAGCACACCCTCTGCGAGAGTGGCAAGAATTTTGGGTCGATGAATATGGATGAGTTCCTCACAAGCATTTGGACCGCCGAAGAAAACCAAGCCATTAATTCCAATCACACCAACACTTCCACTACTAACAACAACACGAACAACATTGAGGTGCACATGCCTTTAGCCGATGCCTCTGCAGATAAGCACATTGCGACGCAGCCCAGCTTGCCGCGACAAGGCTCACTCACACTGCCTGCGCCACTGTGTAGGAAAACGGTGGATGAAGTTTGGTCCGTGATACACAAAGGGCAGCAGGCAAAGCAGCAGAACAATCACAACGGCAGCATCGACGGCGGTGTTCAGAGTTCTGAGTTTGCCCCTCGTCAGCCTACTTTTGGGGAGATGACATTGGAGGATTTTTTGGTTAAAGCAGGGGTAGTTCGGGAACAGGAATCAATGGCAGCCACAGTGGTGCCTCCTCAGCCTCAGCAGCAGCAGCAATATGGGATGTATCAGAACGGCAACCAGGCAGTGGTACCGAGTTTTGTTAATAGGCCTGTGATGGGAATGGGGGCTGCTGGTGCAGCAGGTACTAGCACTGCTACCGGTATTCGGAATTACCAAACTATACCACAAAGTGGGTCTGCGGTTGTGGGAGAGTCCTCTGCGTATGCTGCGAATGGTAAGAGGAATGGGGCTTACCCGACAGTGCCACCTCCACAGTCGGTTTGTTTTGGCGGGAGAGTGGTGAATGGTGGTGATGGATATGCAGCAGGGCAGACAATTGGGATGGGGGCTCTTGTGAGTCCGGTGTCTTCTGATGGGATGGGTACTAGTCAGGTTGAGAACTCTGGGGGTCAATTTGGTTTGGAAATGGGTGGACTAAGAGGAAGGAAGCGGGGTTTAGATGGAGCGGTTGAAAAAGTGGTAGAGAGGAGGCAGAGAAGGATGATTAAGAACAGAGAGTCTGCAGCAAGGTCTAGAGCCCGAAAAACAGGAAATGATCTTCGCCAAGCTCTTAATAGAGTAGTCCAGGCATACACAGTTGAACTGGAAGCAGAACTGAATCAATTGCGAGAAGAGAACTCACACCTTAATCAGGCGCTGAGCTCGAGAGGAAACGAAAGCAACAGTATTTCGAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010152 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0080090 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

447

Amino Acids

47.99

Weight (kDa)

6.73

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 770
Acc65I GGTACC 1 cut(s) 727
AccB1I GGYRCC 2 cut(s) 664, 727
AccB7I CCANNNNNTGG 2 cut(s) 724, 830
AccBSI CCGCTC 1 cut(s) 1106
AccII CGCG 1 cut(s) 424
AciI CCGC 7 cut(s) 285, 422, 546, 839, 930, 1090, 1106
AcsI RAATTY 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 3 cut(s) 729, 784, 1024
AfiI CCNNNNNNNGG 8 cut(s) 109, 428, 587, 724, 755, 804, 830, 1291
AgeI ACCGGT 1 cut(s) 797
AgsI TTSAA 5 cut(s) 10, 37, 50, 1112, 1241
AhlI ACTAGT 1 cut(s) 1025
AjnI CCWGG 2 cut(s) 717, 1224
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 3 cut(s) 417, 1208, 1302
AluI AGCT 3 cut(s) 417, 1208, 1302
Alw21I GWGCWC 2 cut(s) 366, 1304
Alw44I GTGCAC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 1297
Ama87I CYCGRG 1 cut(s) 1303
AoxI GGCC 1 cut(s) 746
ApaLI GTGCAC 1 cut(s) 362
ApoI RAATTY 1 cut(s) 238
AseI ATTAAT 1 cut(s) 306
AsiGI ACCGGT 1 cut(s) 797
Asp718I GGTACC 1 cut(s) 727
AspLEI GCGC 2 cut(s) 451, 1297
AspS9I GGNCC 3 cut(s) 133, 282, 483
AsuHPI GGTGA 3 cut(s) 111, 952, 962
AvaI CYCGRG 1 cut(s) 1303
AvaII GGWCC 3 cut(s) 133, 282, 483
BaeGI GKGCMC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 664, 727
BanII GRGCYC 3 cut(s) 991, 1180, 1304
BbsI GAAGAC 2 cut(s) 178, 999
Bbv12I GWGCWC 2 cut(s) 366, 1304
BbvCI CCTCAGC 2 cut(s) 672, 678
BccI CCATC 7 cut(s) 50, 748, 947, 976, 1007, 1012, 1094
BceAI ACGGC 3 cut(s) 547, 559, 727
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 2 cut(s) 719, 1226
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 3 cut(s) 786, 1026, 1173
BfmI CTRYAG 2 cut(s) 387, 1161
BfoI RGCGCY 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 770
BlpI GCTNAGC 1 cut(s) 1298
Bme1390I CCNGG 2 cut(s) 719, 1226
Bme18I GGWCC 3 cut(s) 133, 282, 483
BmeT110I CYCGRG 1 cut(s) 1303
BmgT120I GGNCC 3 cut(s) 133, 282, 483
BmiI GGNNCC 2 cut(s) 666, 729
BmrFI CCNGG 2 cut(s) 719, 1226
BmsI GCATC 2 cut(s) 370, 546
BpiI GAAGAC 2 cut(s) 178, 999
Bpu10I CCTNAGC 2 cut(s) 672, 678
Bpu1102I GCTNAGC 1 cut(s) 1298
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 797, 1000
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 428, 587, 724, 755, 804, 830, 1291
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 2 cut(s) 151, 1248
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 2 cut(s) 719, 1226
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 6 cut(s) 265, 478, 705, 987, 1023, 1146
BseLI CCNNNNNNNGG 8 cut(s) 109, 428, 587, 724, 755, 804, 830, 1291
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 4 cut(s) 552, 686, 692, 1289
BseNI ACTGG 2 cut(s) 151, 1248
BseRI GAGGAG 2 cut(s) 660, 1141
BseSI GKGCMC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 1 cut(s) 748
BshNI GGYRCC 2 cut(s) 664, 727
BshTI ACCGGT 1 cut(s) 797
BsiHKAI GWGCWC 2 cut(s) 366, 1304
BsiHKCI CYCGRG 1 cut(s) 1303
BsiSI CCGG 2 cut(s) 798, 1001
BslI CCNNNNNNNGG 8 cut(s) 109, 428, 587, 724, 755, 804, 830, 1291
BsnI GGCC 1 cut(s) 748
BsoBI CYCGRG 1 cut(s) 1303
Bsp1286I GDGCHC 4 cut(s) 366, 991, 1180, 1304
Bsp143I GATC 3 cut(s) 15, 61, 1195
Bsp1720I GCTNAGC 1 cut(s) 1298
BspACI CCGC 7 cut(s) 285, 422, 546, 839, 930, 1090, 1106
BspANI GGCC 1 cut(s) 748
BspCNI CTCAG 4 cut(s) 553, 685, 691, 1290
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 666, 729
BspMAI CTGCAG 2 cut(s) 391, 1165
BspMI ACCTGC 1 cut(s) 770
BspT107I GGYRCC 2 cut(s) 664, 727
BsrBI CCGCTC 1 cut(s) 1106
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 2 cut(s) 151, 1248
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 3 cut(s) 15, 61, 1195
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 2 cut(s) 719, 1226
Bst4CI ACNGT 7 cut(s) 456, 469, 661, 901, 915, 1237, 1325
Bst6I CTCTTC 2 cut(s) 166, 1266
BstC8I GCNNGC 3 cut(s) 419, 447, 507
BstDEI CTNAG 5 cut(s) 561, 672, 678, 1076, 1298
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 6 cut(s) 265, 478, 705, 987, 1023, 1146
BstFNI CGCG 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 1298
BstHHI GCGC 2 cut(s) 451, 1297
BstKTI GATC 3 cut(s) 18, 64, 1198
BstMBI GATC 3 cut(s) 15, 61, 1195
BstNI CCWGG 2 cut(s) 719, 1226
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 2 cut(s) 717, 1224
BstSFI CTRYAG 2 cut(s) 387, 1161
BstSLI GKGCMC 1 cut(s) 366
BstUI CGCG 1 cut(s) 424
BstV2I GAAGAC 2 cut(s) 178, 999
BsuRI GGCC 1 cut(s) 748
BtsCI GGATG 6 cut(s) 265, 478, 705, 987, 1023, 1146
BtsI GCAGTG 3 cut(s) 440, 729, 789
BtsIMutI CAGTG 6 cut(s) 440, 452, 666, 729, 789, 906
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 3 cut(s) 419, 447, 507
CaiI CAGNNNCTG 1 cut(s) 1297
CfoI GCGC 2 cut(s) 451, 1297
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 3 cut(s) 133, 282, 483
CseI GACGC 1 cut(s) 415
Csp6I GTAC 3 cut(s) 728, 783, 1023
CspAI ACCGGT 1 cut(s) 797
CviAII CATG 2 cut(s) 73, 367
CviQI GTAC 3 cut(s) 728, 783, 1023
DdeI CTNAG 5 cut(s) 561, 672, 678, 1076, 1298
DpnI GATC 3 cut(s) 17, 63, 1197
DpnII GATC 3 cut(s) 15, 61, 1195
DraIII CACNNNGTG 1 cut(s) 830
Eam1104I CTCTTC 2 cut(s) 166, 1266
EarI CTCTTC 2 cut(s) 166, 1266
Ecl136II GAGCTC 1 cut(s) 1302
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 748
Eco24I GRGCYC 3 cut(s) 991, 1180, 1304
Eco47I GGWCC 3 cut(s) 133, 282, 483
Eco53kI GAGCTC 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 1303
EcoICRI GAGCTC 1 cut(s) 1302
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoRII CCWGG 2 cut(s) 717, 1224
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 3 cut(s) 991, 1180, 1304
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 2 cut(s) 76, 370
FaiI YATR 8 cut(s) 74, 257, 368, 696, 821, 864, 960, 1231
FatI CATG 2 cut(s) 72, 366
FauI CCCGC 2 cut(s) 923, 1083
FokI GGATG 6 cut(s) 272, 485, 712, 994, 1030, 1153
FriOI GRGCYC 3 cut(s) 991, 1180, 1304
FspBI CTAG 3 cut(s) 786, 1026, 1173
GlaI GCGC 2 cut(s) 450, 1296
GsaI CCCAGC 1 cut(s) 417
HaeII RGCGCY 1 cut(s) 1298
HaeIII GGCC 1 cut(s) 748
HapII CCGG 2 cut(s) 798, 1001
HgaI GACGC 1 cut(s) 415
HhaI GCGC 2 cut(s) 451, 1297
Hin1II CATG 2 cut(s) 76, 370
Hin6I GCGC 2 cut(s) 449, 1295
HinP1I GCGC 2 cut(s) 449, 1295
HinfI GANTC 7 cut(s) 86, 116, 644, 851, 997, 1157, 1258
HpaII CCGG 2 cut(s) 798, 1001
HphI GGTGA 3 cut(s) 111, 952, 962
Hpy166II GTNNAC 2 cut(s) 364, 1073
Hpy188I TCNGA 8 cut(s) 85, 91, 133, 555, 562, 708, 807, 1012
Hpy188III TCNNGA 4 cut(s) 200, 635, 1173, 1305
Hpy8I GTNNAC 2 cut(s) 364, 1073
Hpy99I CGWCG 2 cut(s) 409, 545
HpyAV CCTTC 2 cut(s) 1077, 1131
HpyCH4III ACNGT 7 cut(s) 456, 469, 661, 901, 915, 1237, 1325
HpyCH4V TGCA 5 cut(s) 364, 389, 776, 962, 1163
HpyF3I CTNAG 5 cut(s) 561, 672, 678, 1076, 1298
Hsp92II CATG 2 cut(s) 76, 370
HspAI GCGC 2 cut(s) 449, 1295
KpnI GGTACC 1 cut(s) 731
Kzo9I GATC 3 cut(s) 15, 61, 1195
LmnI GCTCC 1 cut(s) 1103
LweI GCATC 2 cut(s) 370, 546
MaeI CTAG 3 cut(s) 786, 1026, 1173
MalI GATC 3 cut(s) 17, 63, 1197
MbiI CCGCTC 1 cut(s) 1106
MboI GATC 3 cut(s) 15, 61, 1195
MboII GAAGA 7 cut(s) 153, 157, 183, 302, 999, 1190, 1283
MfeI CAATTG 2 cut(s) 975, 1262
MhlI GDGCHC 4 cut(s) 366, 991, 1180, 1304
MluCI AATT 8 cut(s) 10, 30, 238, 307, 808, 975, 1052, 1262
MlyI GAGTC 4 cut(s) 95, 860, 1006, 1166
MmeI TCCRAC 1 cut(s) 69
MseI TTAA 6 cut(s) 306, 618, 741, 1146, 1212, 1287
MslI CAYNNNNRTG 3 cut(s) 71, 359, 662
MspI CCGG 2 cut(s) 798, 1001
MspR9I CCNGG 2 cut(s) 719, 1226
MunI CAATTG 2 cut(s) 975, 1262
MvaI CCWGG 2 cut(s) 719, 1226
MvnI CGCG 1 cut(s) 424
NdeII GATC 3 cut(s) 15, 61, 1195
NlaIII CATG 2 cut(s) 76, 370
NlaIV GGNNCC 2 cut(s) 666, 729
NspI RCATGY 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 1303
PceI AGGCCT 1 cut(s) 748
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PfeI GAWTC 3 cut(s) 116, 644, 1258
PflMI CCANNNNNTGG 2 cut(s) 724, 830
PinAI ACCGGT 1 cut(s) 797
PleI GAGTC 4 cut(s) 94, 859, 1005, 1165
PpsI GAGTC 4 cut(s) 94, 859, 1005, 1165
PshBI ATTAAT 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 1304
Psp6I CCWGG 2 cut(s) 717, 1224
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 2 cut(s) 717, 1224
PspN4I GGNNCC 2 cut(s) 666, 729
PspPI GGNCC 3 cut(s) 133, 282, 483
PstI CTGCAG 2 cut(s) 391, 1165
PstNI CAGNNNCTG 1 cut(s) 1297
RsaI GTAC 3 cut(s) 729, 784, 1024
RsaNI GTAC 3 cut(s) 728, 783, 1023
RseI CAYNNNNRTG 3 cut(s) 71, 359, 662
SacI GAGCTC 1 cut(s) 1304
SaqAI TTAA 6 cut(s) 306, 618, 741, 1146, 1212, 1287
Sau3AI GATC 3 cut(s) 15, 61, 1195
Sau96I GGNCC 3 cut(s) 133, 282, 483
SchI GAGTC 4 cut(s) 95, 860, 1006, 1166
ScrFI CCNGG 2 cut(s) 719, 1226
SduI GDGCHC 4 cut(s) 366, 991, 1180, 1304
SfaNI GCATC 2 cut(s) 370, 546
SfcI CTRYAG 2 cut(s) 387, 1161
Sfr274I CTCGAG 1 cut(s) 1303
SinI GGWCC 3 cut(s) 133, 282, 483
SlaI CTCGAG 1 cut(s) 1303
SmiMI CAYNNNNRTG 3 cut(s) 71, 359, 662
SmlI CTYRAG 1 cut(s) 1303
SmoI CTYRAG 1 cut(s) 1303
SpeI ACTAGT 1 cut(s) 1025
Sse9I AATT 8 cut(s) 10, 30, 238, 307, 808, 975, 1052, 1262
SseBI AGGCCT 1 cut(s) 748
SsiI CCGC 7 cut(s) 285, 422, 546, 839, 930, 1090, 1106
SspMI CTAG 3 cut(s) 786, 1026, 1173
SstI GAGCTC 1 cut(s) 1304
StuI AGGCCT 1 cut(s) 748
StyD4I CCNGG 2 cut(s) 717, 1224
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 7 cut(s) 456, 469, 661, 901, 915, 1237, 1325
TaqI TCGA 4 cut(s) 248, 540, 1304, 1331
TasI AATT 8 cut(s) 10, 30, 238, 307, 808, 975, 1052, 1262
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 3 cut(s) 116, 644, 1258
Tru1I TTAA 6 cut(s) 306, 618, 741, 1146, 1212, 1287
Tru9I TTAA 6 cut(s) 306, 618, 741, 1146, 1212, 1287
TscAI CASTG 6 cut(s) 447, 459, 666, 729, 796, 906
TspDTI ATGAA 4 cut(s) 13, 153, 266, 489
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 6 cut(s) 447, 459, 666, 729, 796, 906
Van91I CCANNNNNTGG 2 cut(s) 724, 830
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 3 cut(s) 133, 282, 483
VspI ATTAAT 1 cut(s) 306
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 1 cut(s) 238
XbaI TCTAGA 1 cut(s) 1172
XceI RCATGY 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 1303
XspI CTAG 3 cut(s) 786, 1026, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.