Rorug03G0131100
BZIP Family

Protein ABSCISIC ACID-INSENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006875
Physical Location & Seq
Reverse (-)
10631603 .. 10634050
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0131100.1

Sequence Viewer

Length: 2427 bp
ATGGAAGTTCCCAAGTTTCTCCTCCTTTTGCTATTTGCAGCAACATTTGCTCAGAGTCAAGAACGCTTACAAGGGATGCCAAGCTTAAGGAAAGGGAACTCCTTGAAAGTTGAGGAGGAGACCAACGTCTTGGTTTCACCAGATGGAACTTTTTCTAGTGGATTTTACAAAGTTGGTGCCAATGCCTCCTGTTATTCAATATGGTTCACCAATAGTGCCAATAAAACAATTGTCTGGATGGCTAACAGAGACAAGCCTGTGAGCAAAAGAGGCTCAAAGCTAACCCTCCATGGAAGTGGCAACCTGGTTTTGACAGATGCACTTGGTACACTCGTGTGGTCGACCAACACAATCTCTAATGAAGACATGGAAGTTGTGCTTCTTGACACAGGAAATTTGGTGCTGAGGAATCAAGCAAAGAGGGTAATCTGGCAGAGTTTTGATTTTCCCATAGATACACTTCTACCAACACAGCCACTCACCAAGAACACAACCTTGGTGTCCATGAGAAGCCAAGGTACATACTTATCCGGGTTCTATAACTTCAAATTCGACGATAACAATGTCTTGTTTCTCATCTACAATGGCCCTCAACTTTCTAGTGTTTATTGGCCCCGGATTATTCCAAGTGTTTTTGAAAGTGGTAGAAATCCTTACAACAGCTCTAGGGTGGCAAGTTTAGATGTGGCTGGACAATTTATATCTAGTGACAATATGCAGTTCAATGCATCTGACTACGGGGTTGGTCCAAAGCGGCGTCTAACGGTGGATTATGATGGCATCTTAAGACTGTACAGTCTTCATGAATCGACAGGGTTGTGGAAACTGTCATGGTTGCCTGATGGTGTTGATGCATGCCGGGTTCAGGGCTTGTGCGGTGAATATGGTATTTGCACATACAAACCACAACCTACTTGTACCTGCCCTTCGGGGTTTTCCCTAAACGATCCCTCAGACTGGTCCAAAGGCTGCTCACCACCCTTCAATTTGACTCAACTCACTCCAGATACAAGCAAGCTGGATTTCCTGGAGCTTCCTTACACAGATTACTATGGATATGACATGGCAACCTATACTCGTGGAATCTCTTTTGAAGCATGCAGGAATTCATGTCTAATGCATTCAAGTTGCTTGGGATTCGGATATGCACTAGATGGGATCGGAGAATGTTATCCCAAGAGTCTTCTGCTTAATGGATATCGTATGTCGAATGTTAAGAGCATCATCCATGTGAAGATTCCAAAAGGTTATCTGAACCCTGGTGCAGTCCAAACCAGACTAGAAACACATGATTCGAACTGTTCAGCGGCACAGTTTGTTGTAAGTACTGATCTCGAAACAGAAAAGGGCAATGGAAACCAGTACATGAAATACCTCATTGGATTTTTGAGTTCCTTTGGGCTAATTGAAGCAATCGGCATCAGTTTGACATGGTGGTTTGTGTTCCGAAAGCAAGCTCGTGACGAAATGATGAACATGGGGTATATGGCATTAGCCATAAATGTTGTGGGATTCAAAAGTTTCACCTATGCAGAACTAAAGAAAGCAACCAATGACTTCAAACAGGAGATAGGAAAAGGGGGGTTCGGCAGAGTCTATAAAGGGATTCTGGATGGTGACAGAGTTGTAGCTGTGAAGAGACTAGAAGGCATTGTACAAGGAGATGCAGAGTTTTGGGCAGAGGTTAGTGTCATAGGGAATATCAACCACAGGAACTTGGTTAAGCTTTGGGGTTTCTGTGCTGAGAATGAACATAAGCTGTTGGTATATGAGTATCTGGAGAATGGATCCTTGGATAAACTCTTGTTTTCTGATGTTGAATTAGGATTGGAGCAGCGATACAATATTGCATTAGGCACTGCAAAGGGTTTGTCATATCTACATGAGGAGTGTCTGGAATGGGTACTCCACTGTGATGTAAAGCCTCAAAATATACTGTTAGATGACCATCTTGAACCTAAGGTGGCAGATTTTGGCATGTCAAAGTTATTCAAAGTCAACATTGATGATGCAGGCATTGGATTTTCGAGAGTTCGAGGCACCAGAGGTTATATGGCTCCAGAGTGGATGGTGAACCTTAAAATTGATGCAAAGGCAGATGTCTACAGCTATGGGATTGTTTTGTTGGAGATACTGAGTGGAAAGAGTGCATCTATTCTACTTTCAGCTTTTGCACAAGAGTACACTGAGCACAACCAACTGGTACAATGTGTGACTGAAAAGATCAGAGTCGAAGGGCTTAAGGAAGTAATTGATCCTAGACTCTATCATAATTTCAACAAGAAGAAGCTCGAAAGATTGATGAAGGTTGCTCTTCTGTGTGTGCAAGAAGACCGCAGTGCAAGACCTGCCATGAGCAATGTTGTAGAGCTTCTACTTAAGAATGATGAGGGAAGAGTAGATGATGAAGAATTGAAGATCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009555 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010152 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0080090 GO:0090351 GO:0097159 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

808

Amino Acids

90.6

Weight (kDa)

5.96

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 164 2.7e-25 D-mannose binding lectin
S_locus_glycop PF00954 251 - 313 2.3e-08 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 520 - 792 6e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 521 - 789 2.7e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 795
Acc36I ACCTGC 2 cut(s) 929, 2356
AccB1I GGYRCC 2 cut(s) 176, 2039
AccI GTMKAC 2 cut(s) 341, 2103
AciI CCGC 4 cut(s) 754, 876, 1307, 2335
AclWI GGATC 5 cut(s) 941, 1166, 1782, 1795, 2249
AcsI RAATTY 3 cut(s) 394, 548, 1105
AcyI GRCGYC 1 cut(s) 757
AfiI CCNNNNNNNGG 2 cut(s) 865, 957
AflII CTTAAG 4 cut(s) 85, 784, 2240, 2378
AjnI CCWGG 3 cut(s) 303, 1026, 1258
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 1775, 1807
AleI CACNNNNGTG 1 cut(s) 334
Alw21I GWGCWC 1 cut(s) 2193
Alw26I GTCTC 3 cut(s) 113, 243, 1633
AlwI GGATC 5 cut(s) 941, 1166, 1782, 1795, 2249
AoxI GGCC 2 cut(s) 586, 611
ApeKI GCWGC 3 cut(s) 38, 969, 1834
ApoI RAATTY 3 cut(s) 394, 548, 1105
ArsI GACNNNNNNTTYG 2 cut(s) 1856, 1888
Asp700I GAANNNNTTC 1 cut(s) 151
AspS9I GGNCC 4 cut(s) 587, 612, 746, 960
AsuC2I CCSGG 3 cut(s) 532, 616, 860
AsuHPI GGTGA 8 cut(s) 129, 199, 472, 890, 966, 1516, 1628, 2083
AsuII TTCGAA 1 cut(s) 1295
AvaII GGWCC 2 cut(s) 746, 960
AxyI CCTNAGG 1 cut(s) 1959
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BamHI GGATCC 1 cut(s) 1787
BanI GGYRCC 2 cut(s) 176, 2039
BarI GAAGNNNNNNTAC 6 cut(s) 502, 534, 910, 942, 1638, 1670
BauI CACGAG 3 cut(s) 332, 1077, 1458
BbsI GAAGAC 4 cut(s) 369, 791, 1175, 2337
Bbv12I GWGCWC 1 cut(s) 2193
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 3 cut(s) 50, 956, 1846
BccI CCATC 8 cut(s) 137, 232, 770, 836, 1148, 1607, 1956, 2062
BciT130I CCWGG 3 cut(s) 305, 1028, 1260
BcnI CCSGG 3 cut(s) 532, 616, 860
BcoDI GTCTC 3 cut(s) 113, 243, 1633
BfaI CTAG 8 cut(s) 156, 600, 666, 705, 1151, 1280, 1643, 2259
BfmI CTRYAG 1 cut(s) 2104
BfrI CTTAAG 4 cut(s) 85, 784, 2240, 2378
BfuAI ACCTGC 2 cut(s) 929, 2356
BisI GCNGC 5 cut(s) 39, 755, 970, 1308, 1835
BlsI GCNGC 5 cut(s) 40, 756, 971, 1309, 1836
BmcAI AGTACT 1 cut(s) 1327
Bme1390I CCNGG 6 cut(s) 305, 532, 616, 860, 1028, 1260
Bme18I GGWCC 2 cut(s) 746, 960
BmgT120I GGNCC 4 cut(s) 587, 612, 746, 960
BmiI GGNNCC 5 cut(s) 178, 614, 1789, 2041, 2058
BmrFI CCNGG 6 cut(s) 305, 532, 616, 860, 1028, 1260
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 4 cut(s) 369, 791, 1175, 2337
BpmI CTGGAG 4 cut(s) 987, 1049, 1799, 2043
Bpu10I CCTNAGC 1 cut(s) 404
Bpu14I TTCGAA 1 cut(s) 1295
BpuMI CCSGG 3 cut(s) 532, 616, 860
BsaBI GATNNNNATC 1 cut(s) 1947
BsaHI GRCGYC 1 cut(s) 757
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 289, 495, 514, 614, 1258, 1791
Bsc4I CCNNNNNNNGG 2 cut(s) 865, 957
Bse1I ACTGG 3 cut(s) 962, 1360, 2205
Bse21I CCTNAGG 1 cut(s) 1959
Bse3DI GCAATG 2 cut(s) 1357, 2365
Bse8I GATNNNNATC 1 cut(s) 1947
BseBI CCWGG 3 cut(s) 305, 1028, 1260
BseDI CCNNGG 6 cut(s) 289, 495, 514, 614, 1258, 1791
BseGI GGATG 5 cut(s) 81, 243, 1224, 1618, 2073
BseJI GATNNNNATC 1 cut(s) 1947
BseLI CCNNNNNNNGG 2 cut(s) 865, 957
BseMI GCAATG 2 cut(s) 1357, 2365
BseMII CTCAG 6 cut(s) 65, 395, 966, 1734, 2126, 2178
BseNI ACTGG 3 cut(s) 962, 1360, 2205
BseRI GAGGAG 4 cut(s) 11, 128, 131, 1901
BseXI GCAGC 3 cut(s) 50, 956, 1846
BsgI GTGCAG 1 cut(s) 1284
BshFI GGCC 2 cut(s) 588, 613
BshNI GGYRCC 2 cut(s) 176, 2039
BsiHKAI GWGCWC 1 cut(s) 2193
BsiSI CCGG 3 cut(s) 531, 616, 859
BslI CCNNNNNNNGG 2 cut(s) 865, 957
BsmAI GTCTC 3 cut(s) 113, 243, 1633
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 2 cut(s) 588, 613
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 1295
Bsp1286I GDGCHC 1 cut(s) 2193
Bsp1407I TGTACA 2 cut(s) 792, 1654
Bsp143I GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 4 cut(s) 754, 876, 1307, 2335
BspANI GGCC 2 cut(s) 588, 613
BspCNI CTCAG 6 cut(s) 64, 396, 965, 1735, 2127, 2179
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 5 cut(s) 178, 614, 1789, 2041, 2058
BspMI ACCTGC 2 cut(s) 929, 2356
BspPI GGATC 5 cut(s) 941, 1166, 1782, 1795, 2249
BspQI GCTCTTC 1 cut(s) 2319
BspT104I TTCGAA 1 cut(s) 1295
BspT107I GGYRCC 2 cut(s) 176, 2039
BspTI CTTAAG 4 cut(s) 85, 784, 2240, 2378
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 1357, 2365
BsrGI TGTACA 2 cut(s) 792, 1654
BsrI ACTGG 3 cut(s) 962, 1360, 2205
BssECI CCNNGG 6 cut(s) 289, 495, 514, 614, 1258, 1791
BssMI GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
BssNI GRCGYC 1 cut(s) 757
BssSI CACGAG 3 cut(s) 332, 1077, 1458
BssT1I CCWWGG 4 cut(s) 289, 495, 514, 1791
Bst2BI CACGAG 3 cut(s) 332, 1077, 1458
Bst2UI CCWGG 3 cut(s) 305, 1028, 1260
Bst4CI ACNGT 8 cut(s) 766, 792, 797, 828, 1301, 1314, 1913, 1938
Bst6I CTCTTC 3 cut(s) 1631, 2319, 2389
BstACI GRCGYC 1 cut(s) 757
BstAFI CTTAAG 4 cut(s) 85, 784, 2240, 2378
BstAPI GCANNNNNTGC 2 cut(s) 47, 2348
BstAUI TGTACA 2 cut(s) 792, 1654
BstBI TTCGAA 1 cut(s) 1295
BstC8I GCNNGC 5 cut(s) 856, 1016, 1099, 1455, 2014
BstDEI CTNAG 7 cut(s) 51, 404, 952, 1743, 1959, 2135, 2187
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 5 cut(s) 81, 243, 1224, 1618, 2073
BstKTI GATC 7 cut(s) 949, 1161, 1333, 1790, 2226, 2257, 2421
BstMAI GTCTC 3 cut(s) 113, 243, 1633
BstMBI GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
BstMWI GCNNNNNNNGC 3 cut(s) 47, 270, 2348
BstNI CCWGG 3 cut(s) 305, 1028, 1260
BstNSI RCATGY 3 cut(s) 858, 1101, 1981
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 6 cut(s) 303, 530, 614, 858, 1026, 1258
BstSFI CTRYAG 1 cut(s) 2104
BstV1I GCAGC 3 cut(s) 50, 956, 1846
BstV2I GAAGAC 4 cut(s) 369, 791, 1175, 2337
BstX2I RGATCY 1 cut(s) 1787
BstXI CCANNNNNNTGG 2 cut(s) 130, 296
BstYI RGATCY 1 cut(s) 1787
Bsu36I CCTNAGG 1 cut(s) 1959
BsuRI GGCC 2 cut(s) 588, 613
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 5 cut(s) 81, 243, 1224, 1618, 2073
BtsI GCAGTG 2 cut(s) 1857, 2344
BtsIMutI CAGTG 4 cut(s) 1857, 1909, 2184, 2344
BveI ACCTGC 2 cut(s) 929, 2356
Cac8I GCNNGC 5 cut(s) 856, 1016, 1099, 1455, 2014
CciI TCATGA 1 cut(s) 802
Cfr13I GGNCC 4 cut(s) 587, 612, 746, 960
CseI GACGC 1 cut(s) 746
CsiI ACCWGGT 1 cut(s) 303
DdeI CTNAG 7 cut(s) 51, 404, 952, 1743, 1959, 2135, 2187
DpnI GATC 7 cut(s) 948, 1160, 1332, 1789, 2225, 2256, 2420
DpnII GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
DrdI GACNNNNNNGTC 1 cut(s) 795
DseDI GACNNNNNNGTC 1 cut(s) 795
Eam1104I CTCTTC 3 cut(s) 1631, 2319, 2389
EarI CTCTTC 3 cut(s) 1631, 2319, 2389
Eco130I CCWWGG 4 cut(s) 289, 495, 514, 1791
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 1199
Eco47I GGWCC 2 cut(s) 746, 960
Eco81I CCTNAGG 1 cut(s) 1959
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 3 cut(s) 303, 1026, 1258
EcoRV GATATC 1 cut(s) 1199
EcoT14I CCWWGG 4 cut(s) 289, 495, 514, 1791
EcoT22I ATGCAT 3 cut(s) 730, 856, 1122
ErhI CCWWGG 4 cut(s) 289, 495, 514, 1791
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FauNDI CATATG 1 cut(s) 2423
FblI GTMKAC 2 cut(s) 341, 2103
Fnu4HI GCNGC 5 cut(s) 39, 755, 970, 1308, 1835
FokI GGATG 5 cut(s) 88, 250, 1211, 1625, 2080
Fsp4HI GCNGC 5 cut(s) 39, 755, 970, 1308, 1835
FspBI CTAG 8 cut(s) 156, 600, 666, 705, 1151, 1280, 1643, 2259
GluI GCNGC 5 cut(s) 39, 755, 970, 1308, 1835
GsuI CTGGAG 4 cut(s) 987, 1049, 1799, 2043
HaeIII GGCC 2 cut(s) 588, 613
HapII CCGG 3 cut(s) 531, 616, 859
HgaI GACGC 1 cut(s) 746
Hin1I GRCGYC 1 cut(s) 757
HincII GTYRAC 2 cut(s) 342, 1999
HindII GTYRAC 2 cut(s) 342, 1999
HindIII AAGCTT 2 cut(s) 82, 1724
HpaII CCGG 3 cut(s) 531, 616, 859
HphI GGTGA 8 cut(s) 129, 199, 472, 890, 966, 1516, 1628, 2083
Hpy166II GTNNAC 7 cut(s) 207, 329, 342, 1999, 2074, 2104, 2184
Hpy188I TCNGA 9 cut(s) 54, 733, 955, 1142, 1163, 1254, 1448, 1813, 2228
Hpy8I GTNNAC 7 cut(s) 207, 329, 342, 1999, 2074, 2104, 2184
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 5 cut(s) 936, 991, 1640, 2228, 2299
HpyCH4III ACNGT 8 cut(s) 766, 792, 797, 828, 1301, 1314, 1913, 1938
HpyCH4IV ACGT 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 270, 2348
HpyF3I CTNAG 7 cut(s) 51, 404, 952, 1743, 1959, 2135, 2187
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 757
Kzo9I GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
LguI GCTCTTC 1 cut(s) 2319
LmnI GCTCC 3 cut(s) 1030, 1831, 2062
Lsp1109I GCAGC 3 cut(s) 50, 956, 1846
MabI ACCWGGT 1 cut(s) 303
MaeI CTAG 8 cut(s) 156, 600, 666, 705, 1151, 1280, 1643, 2259
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 4 cut(s) 707, 1460, 1616, 2212
MalI GATC 7 cut(s) 948, 1160, 1332, 1789, 2225, 2256, 2420
MboI GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 1787
MhlI GDGCHC 1 cut(s) 2193
MlyI GAGTC 6 cut(s) 64, 985, 1189, 1602, 2238, 2256
MmeI TCCRAC 1 cut(s) 2106
Mph1103I ATGCAT 3 cut(s) 730, 856, 1122
MroXI GAANNNNTTC 1 cut(s) 151
MseI TTAA 8 cut(s) 86, 785, 1191, 1215, 1722, 2079, 2241, 2379
MslI CAYNNNNRTG 4 cut(s) 294, 334, 1229, 1914
MspA1I CMGCKG 1 cut(s) 1307
MspCI CTTAAG 4 cut(s) 85, 784, 2240, 2378
MspI CCGG 3 cut(s) 531, 616, 859
MspR9I CCNGG 6 cut(s) 305, 532, 616, 860, 1028, 1260
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 3 cut(s) 305, 1028, 1260
MwoI GCNNNNNNNGC 3 cut(s) 47, 270, 2348
NciI CCSGG 3 cut(s) 532, 616, 860
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 2423
NdeII GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
NlaIV GGNNCC 5 cut(s) 178, 614, 1789, 2041, 2058
NmuCI GTSAC 4 cut(s) 707, 1460, 1616, 2212
NsiI ATGCAT 3 cut(s) 730, 856, 1122
NspI RCATGY 3 cut(s) 858, 1101, 1981
NspV TTCGAA 1 cut(s) 1295
OliI CACNNNNGTG 1 cut(s) 334
PaeI GCATGC 2 cut(s) 858, 1101
PagI TCATGA 1 cut(s) 802
PciSI GCTCTTC 1 cut(s) 2319
PctI GAATGC 1 cut(s) 1120
PdmI GAANNNNTTC 1 cut(s) 151
PfeI GAWTC 8 cut(s) 409, 806, 1083, 1137, 1237, 1292, 1512, 1606
PfoI TCCNGGA 1 cut(s) 1026
PkrI GCNGC 5 cut(s) 40, 756, 971, 1309, 1836
PleI GAGTC 6 cut(s) 63, 985, 1188, 1601, 2237, 2256
PpsI GAGTC 6 cut(s) 63, 985, 1188, 1601, 2237, 2256
PshAI GACNNNNGTC 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 303, 1026, 1258
PspGI CCWGG 3 cut(s) 303, 1026, 1258
PspN4I GGNNCC 5 cut(s) 178, 614, 1789, 2041, 2058
PspPI GGNCC 4 cut(s) 587, 612, 746, 960
PsuI RGATCY 1 cut(s) 1787
RseI CAYNNNNRTG 4 cut(s) 294, 334, 1229, 1914
SalI GTCGAC 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 2319
SaqAI TTAA 8 cut(s) 86, 785, 1191, 1215, 1722, 2079, 2241, 2379
SatI GCNGC 5 cut(s) 39, 755, 970, 1308, 1835
Sau3AI GATC 7 cut(s) 946, 1158, 1330, 1787, 2223, 2254, 2418
Sau96I GGNCC 4 cut(s) 587, 612, 746, 960
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 6 cut(s) 64, 985, 1189, 1602, 2238, 2256
ScrFI CCNGG 6 cut(s) 305, 532, 616, 860, 1028, 1260
SduI GDGCHC 1 cut(s) 2193
SexAI ACCWGGT 1 cut(s) 303
SfcI CTRYAG 1 cut(s) 2104
SfuI TTCGAA 1 cut(s) 1295
SinI GGWCC 2 cut(s) 746, 960
SmiMI CAYNNNNRTG 4 cut(s) 294, 334, 1229, 1914
SmlI CTYRAG 4 cut(s) 85, 784, 2240, 2378
SmoI CTYRAG 4 cut(s) 85, 784, 2240, 2378
SphI GCATGC 2 cut(s) 858, 1101
SsiI CCGC 4 cut(s) 754, 876, 1307, 2335
SspI AATATT 1 cut(s) 1846
SspMI CTAG 8 cut(s) 156, 600, 666, 705, 1151, 1280, 1643, 2259
StyD4I CCNGG 6 cut(s) 303, 530, 614, 858, 1026, 1258
StyI CCWWGG 4 cut(s) 289, 495, 514, 1791
TaaI ACNGT 8 cut(s) 766, 792, 797, 828, 1301, 1314, 1913, 1938
TaiI ACGT 1 cut(s) 129
TatI WGTACW 5 cut(s) 792, 1325, 1362, 1654, 2181
TauI GCSGC 2 cut(s) 757, 1310
TfiI GAWTC 8 cut(s) 409, 806, 1083, 1137, 1237, 1292, 1512, 1606
Tru1I TTAA 8 cut(s) 86, 785, 1191, 1215, 1722, 2079, 2241, 2379
Tru9I TTAA 8 cut(s) 86, 785, 1191, 1215, 1722, 2079, 2241, 2379
TscAI CASTG 4 cut(s) 1864, 1916, 2191, 2344
TseFI GTSAC 4 cut(s) 707, 1460, 1616, 2212
TseI GCWGC 3 cut(s) 38, 969, 1834
Tsp45I GTSAC 4 cut(s) 707, 1460, 1616, 2212
TspDTI ATGAA 9 cut(s) 375, 791, 819, 1098, 1382, 1487, 1764, 2318, 2421
TspRI CASTG 4 cut(s) 1864, 1916, 2191, 2344
Vha464I CTTAAG 4 cut(s) 85, 784, 2240, 2378
VpaK11BI GGWCC 2 cut(s) 746, 960
XapI RAATTY 3 cut(s) 394, 548, 1105
XceI RCATGY 3 cut(s) 858, 1101, 1981
XcmI CCANNNNNNNNNTGG 2 cut(s) 1504, 2050
XmiI GTMKAC 2 cut(s) 341, 2103
XmnI GAANNNNTTC 1 cut(s) 151
XspI CTAG 8 cut(s) 156, 600, 666, 705, 1151, 1280, 1643, 2259
ZrmI AGTACT 1 cut(s) 1327
Zsp2I ATGCAT 3 cut(s) 730, 856, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.