MD12G1036900.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010737
Physical Location & Seq
Forward (+)
3990303 .. 3992145
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1036900.v1.1.491

Sequence Viewer

Length: 594 bp
ATGTTGGGAAGCTTGCTTACCAGAATTCTTATATTGTCTCTTGGGTATGCATACCCAGCATTTGAGTGTTATAAAACCGTGGAGAAGAACAGAGTTGAGATTGAAGAACTCCGCTTTTGGTGTCAATATTGGATAATTGTGGCAATGCTTACAGTTCTTGAGAGGGTTGGAGATGTATTCATATCATGGTTGCCCATGTATGGTGAGGGGAAGGTGGCCCTTTTCATCTACCTATGGTATCCAAAGACCCAGGGAACTGGGTTTGTGTACAAGACCTTCCTGAGGCCATATGTAGCAAAGCATGAGACAGACATAGACAGGAAGTTGCTTGAGATGAGAGCAAGAGCCTGGGATTTGACTGTTTTCTACTGGCAGAACTGCGCCCAATACGGCCACACTGCTTTCCTCCAAGCCCTTCAGTACTTGGCTGCTAACTCCACAAACTTCGCCGCCAAAACCACCACCGAGGTAGGCCACATTTACAATGCAATACACTTCTCGGGTTTTTCTAGCATCGATCGAAGTATAGTATGCATCAGAATCCTTGATCGCCCATCCAATCGTTCATTCACCTGGTTCATGCATGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

198

Amino Acids

23.09

Weight (kDa)

8.84

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 6.7e-25 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010737)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42560 AT5G42560 AT5G42560
fragaria_vesca FvH4_6g19930
malus_domestica MD12G1036900.v1.1 MD14G1034500.v1.1 MD14G1035900.v1.1
prunus_persica Prupe.7G098200_v2.0.a1
pyrus_communis pycom14g03170
rosa_chinensis RchiOBHm_Chr3g0475331
rosa_laevigata RLG00000023851
rosa_multiflora Rmu_co8097434.1_g000001 Rmu_ssc0000140.1_g000014
rosa_roxburghii Rroxscaffold_6G00406380
rosa_rugosa Rorug03G0148200
rosa_samantha Rh3AG198100 Rh3BG228300 Rh3CG222600 Rh3DG223500
rosa_wichuraiana Rw3G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AciI CCGC 2 cut(s) 112, 450
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 269, 422
AfiI CCNNNNNNNGG 2 cut(s) 200, 282
AgsI TTSAA 1 cut(s) 104
AjnI CCWGG 3 cut(s) 249, 347, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 42, 299
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 4 cut(s) 216, 284, 391, 472
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 24
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 215, 562
AvaI CYCGRG 1 cut(s) 499
AxyI CCTNAGG 1 cut(s) 281
BarI GAAGNNNNNNTAC 3 cut(s) 33, 260, 292
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 562
BceAI ACGGC 1 cut(s) 406
BciT130I CCWGG 3 cut(s) 251, 349, 574
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 2 cut(s) 42, 299
BfaI CTAG 1 cut(s) 510
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 2 cut(s) 429, 450
BlsI GCNGC 2 cut(s) 430, 451
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 3 cut(s) 251, 349, 574
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 251, 349, 574
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 2 cut(s) 522, 543
BmuI ACTGGG 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 179, 350
Bsa29I ATCGAT 1 cut(s) 516
BsaJI CCNNGG 5 cut(s) 78, 249, 250, 348, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 282
Bse1I ACTGG 2 cut(s) 262, 374
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 3 cut(s) 251, 349, 574
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 5 cut(s) 78, 249, 250, 348, 465
BseGI GGATG 1 cut(s) 554
BseLI CCNNNNNNNGG 2 cut(s) 200, 282
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 262, 374
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 520
BshFI GGCC 4 cut(s) 218, 286, 393, 474
BshVI ATCGAT 1 cut(s) 516
BsiEI CGRYCG 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 499
BslI CCNNNNNNNGG 2 cut(s) 200, 282
BsmAI GTCTC 2 cut(s) 42, 299
BsnI GGCC 4 cut(s) 218, 286, 393, 474
BsoBI CYCGRG 1 cut(s) 499
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 517, 547
BspACI CCGC 2 cut(s) 112, 450
BspANI GGCC 4 cut(s) 218, 286, 393, 474
BspCNI CTCAG 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 262, 374
BssECI CCNNGG 5 cut(s) 78, 249, 250, 348, 465
BssMI GATC 2 cut(s) 517, 547
Bst2UI CCWGG 3 cut(s) 251, 349, 574
Bst4CI ACNGT 3 cut(s) 79, 154, 361
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 14, 585
BstDEI CTNAG 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 2 cut(s) 520, 550
BstMAI GTCTC 2 cut(s) 42, 299
BstMBI GATC 2 cut(s) 517, 547
BstMCI CGRYCG 1 cut(s) 520
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 3 cut(s) 251, 349, 574
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 249, 347, 572
BstV1I GCAGC 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 257
Bsu15I ATCGAT 1 cut(s) 516
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 4 cut(s) 218, 286, 393, 474
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 554
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 14, 585
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 217
ClaI ATCGAT 1 cut(s) 516
CsiI ACCWGGT 1 cut(s) 572
Csp6I GTAC 2 cut(s) 268, 421
CviAII CATG 6 cut(s) 186, 196, 302, 580, 584, 589
CviJI RGCY 8 cut(s) 12, 218, 286, 347, 393, 413, 428, 474
CviKI_1 RGCY 8 cut(s) 12, 218, 286, 347, 393, 413, 428, 474
CviQI GTAC 2 cut(s) 268, 421
DdeI CTNAG 1 cut(s) 281
DpnI GATC 2 cut(s) 519, 549
DpnII GATC 2 cut(s) 517, 547
EaeI YGGCCR 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 3 cut(s) 249, 347, 572
EcoT22I ATGCAT 3 cut(s) 52, 536, 585
FaeI CATG 6 cut(s) 189, 199, 305, 583, 587, 592
FatI CATG 6 cut(s) 185, 195, 301, 579, 583, 588
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 429, 450
FokI GGATG 1 cut(s) 541
Fsp4HI GCNGC 2 cut(s) 429, 450
FspBI CTAG 1 cut(s) 510
GlaI GCGC 1 cut(s) 382
GluI GCNGC 2 cut(s) 429, 450
GsaI CCCAGC 1 cut(s) 59
HaeIII GGCC 4 cut(s) 218, 286, 393, 474
HhaI GCGC 1 cut(s) 383
Hin1II CATG 6 cut(s) 189, 199, 305, 583, 587, 592
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 1 cut(s) 540
HphI GGTGA 2 cut(s) 215, 562
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 539
Hpy188III TCNNGA 2 cut(s) 158, 280
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 205, 286, 425
HpyCH4III ACNGT 3 cut(s) 79, 154, 361
HpyCH4V TGCA 4 cut(s) 50, 488, 534, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 1 cut(s) 281
Hsp92II CATG 6 cut(s) 189, 199, 305, 583, 587, 592
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 2 cut(s) 517, 547
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 522, 543
MabI ACCWGGT 1 cut(s) 572
MaeI CTAG 1 cut(s) 510
MalI GATC 2 cut(s) 519, 549
MboI GATC 2 cut(s) 517, 547
MboII GAAGA 2 cut(s) 97, 116
MluCI AATT 2 cut(s) 24, 135
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 5 cut(s) 156, 199, 276, 416, 460
Mph1103I ATGCAT 3 cut(s) 52, 536, 585
MslI CAYNNNNRTG 1 cut(s) 64
MspR9I CCNGG 3 cut(s) 251, 349, 574
MvaI CCWGG 3 cut(s) 251, 349, 574
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 517, 547
NlaIII CATG 6 cut(s) 189, 199, 305, 583, 587, 592
NsiI ATGCAT 3 cut(s) 52, 536, 585
NspI RCATGY 1 cut(s) 587
PaeI GCATGC 1 cut(s) 587
PasI CCCWGGG 1 cut(s) 250
PfeI GAWTC 1 cut(s) 540
PkrI GCNGC 2 cut(s) 430, 451
Ple19I CGATCG 1 cut(s) 520
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 3 cut(s) 249, 347, 572
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 3 cut(s) 249, 347, 572
PspPI GGNCC 1 cut(s) 217
PvuI CGATCG 1 cut(s) 520
RsaI GTAC 2 cut(s) 269, 422
RsaNI GTAC 2 cut(s) 268, 421
RseI CAYNNNNRTG 1 cut(s) 64
SatI GCNGC 2 cut(s) 429, 450
Sau3AI GATC 2 cut(s) 517, 547
Sau96I GGNCC 1 cut(s) 217
ScaI AGTACT 1 cut(s) 422
ScrFI CCNGG 3 cut(s) 251, 349, 574
SetI ASST 6 cut(s) 14, 216, 234, 278, 471, 575
SexAI ACCWGGT 1 cut(s) 572
SfaNI GCATC 2 cut(s) 522, 543
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 158, 329
SmoI CTYRAG 2 cut(s) 158, 329
SphI GCATGC 1 cut(s) 587
Sse9I AATT 2 cut(s) 24, 135
SsiI CCGC 2 cut(s) 112, 450
SspI AATATT 1 cut(s) 128
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 249, 347, 572
TaaI ACNGT 3 cut(s) 79, 154, 361
TaqI TCGA 2 cut(s) 516, 520
TasI AATT 2 cut(s) 24, 135
TatI WGTACW 2 cut(s) 267, 420
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 1 cut(s) 540
TscAI CASTG 1 cut(s) 403
TseI GCWGC 1 cut(s) 428
TspDTI ATGAA 4 cut(s) 169, 214, 555, 568
TspRI CASTG 1 cut(s) 403
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 587
XspI CTAG 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 3 cut(s) 52, 536, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.