MD14G1035900.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010737
Physical Location & Seq
Forward (+)
3244470 .. 3247452
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1035900.v1.1.491

Sequence Viewer

Length: 714 bp
ATGTTGGGAAGCTTGCTAACCAGAATTCTTATATTGTTTCTTGGGTATGCATACCCAGCATTTGAGTGCTACAAAACAGTGGAGAAGAACAGAGTTGAGATTGAAGAACTCCGGTTTTGGTGTCAATACTGGATAATTGTGGCAATGCTCACAGTTCTTGAGAGGGCTGGAGATGTATTCATCTCATGGTTGCCCATGTATGGTGAAGGGAAGGTGGCCCTTTTTATATACCTATGGTGTCCAAAGACCAAGGGAACTTTCTTTGTGTACAAGACCTTCCTGAGGCCATATGTAGCAAAGCATGAGACAGACATAGACAGGAAGTTGCTGGAGATGAGAGCAAGAGCCTGGGATTTGACGGTACTCTACTGGCAGAACTTCGCCCAATATGGCCACGCTGCTTTCCTCCAAGCCCTTCGGTACATGGCTGCTAACTCCGCAAAGTTCGCCACCAAACCCACCACCGAGTCGCAGACAGTCGATGATGATCAGCAGGACACAGCAGCGCCGCTACGTAAACCGAGCGGGAAGAACAAGTGGCAGCCAGGATCCCCACCAGCAACACCAAATGGCAGAGCCACCACAGTCAACCGCGCCATGTCTGAGACTCCGAGGTCCCCCAAGGTGGTTCATCCCCGACATCAAACGGATGGTGGGCAGGTTGACGAGTTGACTGAGAAGCTCCGGCTCAGGCGTTCTAAGCCAATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

238

Amino Acids

27.36

Weight (kDa)

9.51

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 19 - 97 1.5e-23 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010737)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42560 AT5G42560 AT5G42560
fragaria_vesca FvH4_6g19930
malus_domestica MD12G1036900.v1.1 MD14G1034500.v1.1 MD14G1035900.v1.1
prunus_persica Prupe.7G098200_v2.0.a1
pyrus_communis pycom14g03170
rosa_chinensis RchiOBHm_Chr3g0475331
rosa_laevigata RLG00000023851
rosa_multiflora Rmu_co8097434.1_g000001 Rmu_ssc0000140.1_g000014
rosa_roxburghii Rroxscaffold_6G00406380
rosa_rugosa Rorug03G0148200
rosa_samantha Rh3AG198100 Rh3BG228300 Rh3CG222600 Rh3DG223500
rosa_wichuraiana Rw3G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 649
AccBSI CCGCTC 1 cut(s) 525
AccII CGCG 1 cut(s) 594
AciI CCGC 4 cut(s) 438, 509, 525, 592
AclWI GGATC 2 cut(s) 543, 556
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 24
AfaI GTAC 3 cut(s) 269, 363, 422
AfiI CCNNNNNNNGG 3 cut(s) 200, 282, 625
AgsI TTSAA 1 cut(s) 104
AjnI CCWGG 2 cut(s) 347, 544
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 2 cut(s) 12, 682
AluI AGCT 2 cut(s) 12, 682
Alw26I GTCTC 2 cut(s) 299, 599
AlwI GGATC 2 cut(s) 543, 556
AoxI GGCC 3 cut(s) 216, 284, 391
ApeKI GCWGC 4 cut(s) 398, 428, 503, 541
ApoI RAATTY 1 cut(s) 24
AspLEI GCGC 2 cut(s) 508, 596
AspS9I GGNCC 2 cut(s) 217, 615
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 615
AxyI CCTNAGG 1 cut(s) 281
BalI TGGCCA 1 cut(s) 393
BamHI GGATCC 1 cut(s) 548
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbvI GCAGC 4 cut(s) 385, 415, 515, 553
BccI CCATC 1 cut(s) 644
BciT130I CCWGG 2 cut(s) 349, 546
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 2 cut(s) 299, 599
BfoI RGCGCY 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 649
BisI GCNGC 5 cut(s) 399, 429, 504, 509, 542
BlsI GCNGC 5 cut(s) 400, 430, 505, 510, 543
Bme1390I CCNGG 2 cut(s) 349, 546
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 2 cut(s) 217, 615
BmiI GGNNCC 2 cut(s) 550, 617
BmrFI CCNGG 2 cut(s) 349, 546
BpmI CTGGAG 2 cut(s) 189, 350
Bpu10I CCTNAGC 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 4 cut(s) 249, 348, 611, 621
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 282, 625
Bse1I ACTGG 3 cut(s) 134, 374, 709
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 2 cut(s) 349, 546
BseDI CCNNGG 4 cut(s) 249, 348, 611, 621
BseGI GGATG 2 cut(s) 631, 655
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 200, 282, 625
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 4 cut(s) 272, 594, 666, 703
BseNI ACTGG 3 cut(s) 134, 374, 709
BseXI GCAGC 4 cut(s) 385, 415, 515, 553
BseYI CCCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 594
BshFI GGCC 3 cut(s) 218, 286, 393
BsiSI CCGG 2 cut(s) 112, 685
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 3 cut(s) 200, 282, 625
BsmAI GTCTC 2 cut(s) 299, 599
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 3 cut(s) 218, 286, 393
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 487, 548
BspACI CCGC 4 cut(s) 438, 509, 525, 592
BspANI GGCC 3 cut(s) 218, 286, 393
BspCNI CTCAG 4 cut(s) 273, 595, 667, 702
BspFNI CGCG 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 550, 617
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 2 cut(s) 543, 556
BsrBI CCGCTC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 3 cut(s) 134, 374, 709
BssECI CCNNGG 4 cut(s) 249, 348, 611, 621
BssMI GATC 2 cut(s) 487, 548
BssT1I CCWWGG 2 cut(s) 249, 621
Bst2UI CCWGG 2 cut(s) 349, 546
Bst4CI ACNGT 5 cut(s) 79, 154, 361, 478, 586
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 5 cut(s) 281, 603, 675, 689, 699
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 631, 655
BstFNI CGCG 1 cut(s) 594
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 508, 596
BstKTI GATC 2 cut(s) 490, 551
BstMAI GTCTC 2 cut(s) 299, 599
BstMBI GATC 2 cut(s) 487, 548
BstMWI GCNNNNNNNGC 4 cut(s) 56, 437, 446, 700
BstNI CCWGG 2 cut(s) 349, 546
BstSCI CCNGG 2 cut(s) 347, 544
BstSNI TACGTA 1 cut(s) 515
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 4 cut(s) 385, 415, 515, 553
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
Bsu36I CCTNAGG 1 cut(s) 281
BsuRI GGCC 3 cut(s) 218, 286, 393
BtsCI GGATG 2 cut(s) 631, 655
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 1 cut(s) 14
CfoI GCGC 2 cut(s) 508, 596
Cfr13I GGNCC 2 cut(s) 217, 615
Csp6I GTAC 3 cut(s) 268, 362, 421
CviAII CATG 5 cut(s) 186, 196, 302, 424, 598
CviQI GTAC 3 cut(s) 268, 362, 421
DdeI CTNAG 5 cut(s) 281, 603, 675, 689, 699
DpnI GATC 2 cut(s) 489, 550
DpnII GATC 2 cut(s) 487, 548
DrdI GACNNNNNNGTC 1 cut(s) 613
DseDI GACNNNNNNGTC 1 cut(s) 613
EaeI YGGCCR 1 cut(s) 391
Eco105I TACGTA 1 cut(s) 515
Eco130I CCWWGG 2 cut(s) 249, 621
Eco47I GGWCC 1 cut(s) 615
Eco81I CCTNAGG 1 cut(s) 281
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 347, 544
EcoT14I CCWWGG 2 cut(s) 249, 621
EcoT22I ATGCAT 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 249, 621
FaeI CATG 5 cut(s) 189, 199, 305, 427, 601
FaqI GGGAC 1 cut(s) 601
FatI CATG 5 cut(s) 185, 195, 301, 423, 597
FauI CCCGC 1 cut(s) 518
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 5 cut(s) 399, 429, 504, 509, 542
FokI GGATG 2 cut(s) 618, 662
Fsp4HI GCNGC 5 cut(s) 399, 429, 504, 509, 542
GlaI GCGC 2 cut(s) 507, 595
GluI GCNGC 5 cut(s) 399, 429, 504, 509, 542
GsaI CCCAGC 1 cut(s) 59
GsuI CTGGAG 2 cut(s) 189, 350
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 3 cut(s) 218, 286, 393
HapII CCGG 2 cut(s) 112, 685
HhaI GCGC 2 cut(s) 508, 596
Hin1II CATG 5 cut(s) 189, 199, 305, 427, 601
Hin6I GCGC 2 cut(s) 506, 594
HinP1I GCGC 2 cut(s) 506, 594
HincII GTYRAC 3 cut(s) 589, 664, 672
HindII GTYRAC 3 cut(s) 589, 664, 672
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 2 cut(s) 467, 607
HpaII CCGG 2 cut(s) 112, 685
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 5 cut(s) 268, 518, 589, 664, 672
Hpy188I TCNGA 2 cut(s) 604, 612
Hpy188III TCNNGA 2 cut(s) 158, 280
Hpy8I GTNNAC 5 cut(s) 268, 518, 589, 664, 672
HpyAV CCTTC 4 cut(s) 200, 205, 286, 425
HpyCH4III ACNGT 5 cut(s) 79, 154, 361, 478, 586
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 437, 446, 700
HpyF3I CTNAG 5 cut(s) 281, 603, 675, 689, 699
HpySE526I ACGT 1 cut(s) 514
Hsp92II CATG 5 cut(s) 189, 199, 305, 427, 601
HspAI GCGC 2 cut(s) 506, 594
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 2 cut(s) 487, 548
LmnI GCTCC 1 cut(s) 687
Lsp1109I GCAGC 4 cut(s) 385, 415, 515, 553
MaeII ACGT 1 cut(s) 514
MalI GATC 2 cut(s) 489, 550
MbiI CCGCTC 1 cut(s) 525
MboI GATC 2 cut(s) 487, 548
MboII GAAGA 3 cut(s) 97, 116, 541
MflI RGATCY 1 cut(s) 548
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 2 cut(s) 24, 135
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 2 cut(s) 476, 601
MnlI CCTC 4 cut(s) 156, 276, 416, 606
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 1 cut(s) 52
MscI TGGCCA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 393
MspI CCGG 2 cut(s) 112, 685
MspR9I CCNGG 2 cut(s) 349, 546
MvaI CCWGG 2 cut(s) 349, 546
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 4 cut(s) 56, 437, 446, 700
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 487, 548
NlaIII CATG 5 cut(s) 189, 199, 305, 427, 601
NlaIV GGNNCC 2 cut(s) 550, 617
NsiI ATGCAT 1 cut(s) 52
PkrI GCNGC 5 cut(s) 400, 430, 505, 510, 543
PleI GAGTC 2 cut(s) 475, 601
PpsI GAGTC 2 cut(s) 475, 601
Ppu21I YACGTR 1 cut(s) 515
PpuMI RGGWCCY 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 615
Psp6I CCWGG 2 cut(s) 347, 544
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 2 cut(s) 347, 544
PspN4I GGNNCC 2 cut(s) 550, 617
PspPI GGNCC 2 cut(s) 217, 615
PspPPI RGGWCCY 1 cut(s) 615
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 3 cut(s) 269, 363, 422
RsaNI GTAC 3 cut(s) 268, 362, 421
RseI CAYNNNNRTG 1 cut(s) 64
SatI GCNGC 5 cut(s) 399, 429, 504, 509, 542
Sau3AI GATC 2 cut(s) 487, 548
Sau96I GGNCC 2 cut(s) 217, 615
SchI GAGTC 2 cut(s) 476, 601
ScrFI CCNGG 2 cut(s) 349, 546
SetI ASST 9 cut(s) 14, 216, 234, 278, 517, 617, 627, 663, 684
SinI GGWCC 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SnaBI TACGTA 1 cut(s) 515
Sse9I AATT 2 cut(s) 24, 135
SsiI CCGC 4 cut(s) 438, 509, 525, 592
StyD4I CCNGG 2 cut(s) 347, 544
StyI CCWWGG 2 cut(s) 249, 621
TaaI ACNGT 5 cut(s) 79, 154, 361, 478, 586
TaiI ACGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 480
TasI AATT 2 cut(s) 24, 135
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 511
TscAI CASTG 1 cut(s) 84
TseI GCWGC 4 cut(s) 398, 428, 503, 541
TspDTI ATGAA 2 cut(s) 169, 620
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.