MD12G1042900.v1.1

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013093
Physical Location & Seq
Forward (+)
4803576 .. 4804394
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1042900.v1.1.491

Sequence Viewer

Length: 819 bp
ATGAGAGGAAAGTTTCTCAAGAAATTGAAGGCCATCCCAACAATTAACACTTTGAAACATGGCCTGCTCTTTCAGCTCGATCCTGCGGAAAAGTTCTCCAATCCGAACCACCAAACACTGTCTCAGCTCGTTCGCAAAAATGAAGATTGTCAGAAGAAGAACACTCTATCGGCGGAGCTTGTATTTAAAGAGCTCAAAGATGGAGATACGGGACTGGGTTCTAATGTCTGTAAGGAGAATATTACACCGCCCTCAATGGAAACCAAAGAAACAGTTATGGAGGATGACATTTTTCATGATGCAGAGGATGATGATGAACTTCCGTCTTTGTTCGATTTCGAGGAAAAGTGTCCGCCAGGAGGAGGCGATTGTGTCGTTCTATATTCAACAAGCGTGACAGGGGTTAGGAAAACGTTCGAGGACTGCAAGGCAATCCGGTTTTTGCTGGAGAGTTTCAAAGTACCGGTGTATGAGAGGGACCTTTCGATGCACATGGAATTCAGGGAAGAATTGTGGAGGATATTTGGCGGTAGAGTGATCCCTCCGAGGCTTTTCATCAAGGGAAGGTACATTGGTGGAGCTGAAGAAGTTACAGCACTTCATGAGCAAGGAAAGCTGAAAAAGCTCTTCGAAGGAATCCCAATTGACCTCTCTAGCTCCCCATGCACCGGCTGTGCCAACGTGCGCTTCGTTGTGTGCTTCAATTGCAATGGCAGTCGCAAAGTTTTCACGGATGGTGATCACGAAAACGATGAGTTGTGCATTAGATGTCCGGAGTGCAACGAAAATGGTTTGATCAAGTGCTCCATCTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

273

Amino Acids

30.66

Weight (kDa)

5.41

Isoelectric Point (pI)

26.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 125 - 191 2.6e-12 Glutaredoxin
GRXCR1-2_C PF23733 222 - 272 5.8e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11773 AT3G11773 AT5G06470
fragaria_vesca FvH4_6g20660
malus_domestica MD12G1042900.v1.1 MD14G1041800.v1.1
prunus_persica Prupe.7G092000_v2.0.a1
pyrus_communis pycom14g03560
rosa_chinensis RchiOBHm_Chr3g0476541
rosa_laevigata RLG00000023763
rosa_multiflora Rmu_sc0004556.1_g000002
rosa_roxburghii Rroxscaffold_6G00405240
rosa_rugosa Rorug03G0154700
rosa_samantha Rh3AG205800 Rh3BG238200 Rh3CG232000 Rh3DG231900
rosa_wichuraiana Rw3G018730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 772
AciI CCGC 5 cut(s) 86, 173, 248, 353, 528
AclI AACGTT 1 cut(s) 413
AclWI GGATC 2 cut(s) 74, 532
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 462, 569
AfiI CCNNNNNNNGG 3 cut(s) 359, 362, 668
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 5 cut(s) 28, 55, 387, 457, 703
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 8 cut(s) 76, 127, 178, 193, 581, 616, 625, 657
AluI AGCT 8 cut(s) 76, 127, 178, 193, 581, 616, 625, 657
Alw21I GWGCWC 2 cut(s) 195, 806
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 2 cut(s) 74, 532
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 2 cut(s) 30, 61
ApeKI GCWGC 1 cut(s) 813
ApoI RAATTY 1 cut(s) 497
AsiGI ACCGGT 1 cut(s) 463
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 1 cut(s) 478
AsuHPI GGTGA 1 cut(s) 749
AsuII TTCGAA 1 cut(s) 630
AvaII GGWCC 1 cut(s) 478
BaeI ACNNNNGTAYC 2 cut(s) 559, 592
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 2 cut(s) 195, 806
BbvI GCAGC 1 cut(s) 800
BccI CCATC 4 cut(s) 41, 194, 728, 815
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 2 cut(s) 739, 795
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 654
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 1 cut(s) 357
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 289, 477
BmuI ACTGGG 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 630
BsaBI GATNNNNATC 1 cut(s) 738
BsaJI CCNNGG 1 cut(s) 545
BsaWI WCCGGW 3 cut(s) 435, 463, 772
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 362, 668
Bse118I RCCGGY 2 cut(s) 463, 668
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 715
Bse8I GATNNNNATC 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 772
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 545
BseGI GGATG 4 cut(s) 33, 289, 313, 739
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 359, 362, 668
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 375
BseXI GCAGC 1 cut(s) 800
BshFI GGCC 2 cut(s) 32, 63
BshTI ACCGGT 1 cut(s) 463
BsiHKAI GWGCWC 2 cut(s) 195, 806
BsiSI CCGG 4 cut(s) 436, 464, 669, 773
BslFI GGGAC 2 cut(s) 225, 491
BslI CCNNNNNNNGG 3 cut(s) 359, 362, 668
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 2 cut(s) 225, 491
BsnI GGCC 2 cut(s) 32, 63
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 195, 806
Bsp13I TCCGGA 1 cut(s) 772
Bsp143I GATC 4 cut(s) 79, 537, 739, 795
BspACI CCGC 5 cut(s) 86, 173, 248, 353, 528
BspANI GGCC 2 cut(s) 32, 63
BspCNI CTCAG 1 cut(s) 136
BspEI TCCGGA 1 cut(s) 772
BspHI TCATGA 2 cut(s) 295, 601
BspLI GGNNCC 1 cut(s) 479
BspPI GGATC 2 cut(s) 74, 532
BspQI GCTCTTC 1 cut(s) 632
BspT104I TTCGAA 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 2 cut(s) 463, 668
BsrI ACTGG 1 cut(s) 219
BssAI RCCGGY 2 cut(s) 463, 668
BssECI CCNNGG 1 cut(s) 545
BssMI GATC 4 cut(s) 79, 537, 739, 795
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 120, 274
Bst6I CTCTTC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 4 cut(s) 33, 289, 313, 739
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 4 cut(s) 82, 540, 742, 798
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 4 cut(s) 79, 537, 739, 795
BstMWI GCNNNNNNNGC 6 cut(s) 73, 613, 622, 663, 705, 810
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 800
BsuRI GGCC 2 cut(s) 32, 63
BtsCI GGATG 4 cut(s) 33, 289, 313, 739
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 65
CciI TCATGA 2 cut(s) 295, 601
CfoI GCGC 1 cut(s) 687
Cfr10I RCCGGY 2 cut(s) 463, 668
Cfr13I GGNCC 1 cut(s) 478
Csp6I GTAC 2 cut(s) 461, 568
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 5 cut(s) 59, 296, 493, 602, 663
CviQI GTAC 2 cut(s) 461, 568
DdeI CTNAG 1 cut(s) 123
DpnI GATC 4 cut(s) 81, 539, 741, 797
DpnII GATC 4 cut(s) 79, 537, 739, 795
DraI TTTAAA 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
EciI GGCGGA 2 cut(s) 188, 342
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 5 cut(s) 62, 299, 496, 605, 666
FaiI YATR 8 cut(s) 60, 278, 297, 382, 471, 494, 603, 664
FaqI GGGAC 2 cut(s) 225, 491
FatI CATG 5 cut(s) 58, 295, 492, 601, 662
FbaI TGATCA 2 cut(s) 739, 795
Fnu4HI GCNGC 1 cut(s) 814
FokI GGATG 4 cut(s) 20, 296, 320, 746
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 1 cut(s) 814
FspBI CTAG 1 cut(s) 654
GlaI GCGC 1 cut(s) 686
GluI GCNGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 2 cut(s) 32, 63
HapII CCGG 4 cut(s) 436, 464, 669, 773
HhaI GCGC 1 cut(s) 687
Hin1II CATG 5 cut(s) 62, 299, 496, 605, 666
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HinfI GANTC 1 cut(s) 636
HpaII CCGG 4 cut(s) 436, 464, 669, 773
HphI GGTGA 1 cut(s) 749
Hpy188I TCNGA 3 cut(s) 105, 153, 546
Hpy188III TCNNGA 5 cut(s) 19, 296, 602, 743, 773
HpyAV CCTTC 3 cut(s) 22, 558, 626
HpyCH4III ACNGT 2 cut(s) 120, 274
HpyCH4IV ACGT 2 cut(s) 413, 681
HpyCH4V TGCA 7 cut(s) 302, 426, 490, 666, 708, 762, 780
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 613, 622, 663, 705, 810
HpyF3I CTNAG 1 cut(s) 123
HpySE526I ACGT 2 cut(s) 413, 681
Hsp92II CATG 5 cut(s) 62, 299, 496, 605, 666
HspAI GCGC 1 cut(s) 685
Kpn2I TCCGGA 1 cut(s) 772
Ksp22I TGATCA 2 cut(s) 739, 795
Kzo9I GATC 4 cut(s) 79, 537, 739, 795
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 4 cut(s) 175, 578, 662, 809
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 2 cut(s) 289, 477
MaeI CTAG 1 cut(s) 654
MaeII ACGT 2 cut(s) 413, 681
MaeIII GTNAC 2 cut(s) 394, 589
MalI GATC 4 cut(s) 81, 539, 741, 797
MboI GATC 4 cut(s) 79, 537, 739, 795
MboII GAAGA 6 cut(s) 155, 166, 169, 518, 596, 619
MfeI CAATTG 2 cut(s) 642, 703
MhlI GDGCHC 2 cut(s) 195, 806
MluCI AATT 6 cut(s) 23, 42, 497, 509, 642, 703
MroI TCCGGA 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 2 cut(s) 45, 186
MspI CCGG 4 cut(s) 436, 464, 669, 773
MspR9I CCNGG 1 cut(s) 357
MunI CAATTG 2 cut(s) 642, 703
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 6 cut(s) 73, 613, 622, 663, 705, 810
NdeII GATC 4 cut(s) 79, 537, 739, 795
NlaIII CATG 5 cut(s) 62, 299, 496, 605, 666
NlaIV GGNNCC 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 394
NspV TTCGAA 1 cut(s) 630
PagI TCATGA 2 cut(s) 295, 601
PciSI GCTCTTC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 636
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 1 cut(s) 815
PpuMI RGGWCCY 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 195
Psp1406I AACGTT 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 1 cut(s) 478
PspPPI RGGWCCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 462, 569
RsaNI GTAC 2 cut(s) 461, 568
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 2 cut(s) 45, 186
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 4 cut(s) 79, 537, 739, 795
Sau96I GGNCC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 195, 806
SfaNI GCATC 2 cut(s) 289, 477
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 1 cut(s) 478
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 6 cut(s) 23, 42, 497, 509, 642, 703
SsiI CCGC 5 cut(s) 86, 173, 248, 353, 528
SspI AATATT 1 cut(s) 241
SspMI CTAG 1 cut(s) 654
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 355
TaaI ACNGT 2 cut(s) 120, 274
TaiI ACGT 2 cut(s) 416, 684
TaqI TCGA 6 cut(s) 78, 333, 339, 417, 485, 630
TasI AATT 6 cut(s) 23, 42, 497, 509, 642, 703
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 2 cut(s) 45, 186
Tru9I TTAA 2 cut(s) 45, 186
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 813
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 5 cut(s) 156, 284, 330, 544, 590
TspGWI ACGGA 2 cut(s) 312, 746
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 478
XapI RAATTY 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.