MD12G1049900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014711
Physical Location & Seq
Forward (+)
5661520 .. 5664276
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1049900.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGCACGTAGAAATTATGAAGCCATTTTTGTGGGTGGTCCCAATTTTGTTGTTGGTTTCAATCTTACATTATTCTTGTTTTGTTGATGGGGCACCACAAGTTCCATGTTACTTCATATTTGGAGACTCATTATCTGACAGTGGAAACAACAATGGCCTTTTAACGTTGGCTAAAGCAAATTATAAACCCTACGGCATTGATTTTCCAAGAGGTCCAACCGGAAGGTTTTCCAATGGACGTAATTTGGTTGATGTTATTGCTGAATTACTAGGCTTTAGTCATTACATTCCACCTTTTGCTGCTGCAATAGGATGGAAAATACTCCACGGTGTGAATTACGCATCCGGTGCAGCAGGTATTCGTGATGAATCGGGACGGAACCAGGGTATGAGAGTAACCTTTAATAAGCAGTTGGTGAATCACCAAAACATAGTAAGACAGATTGTTTCCCTTCAGGGAAATAATAATTCAGCAGCTATCCAGTACCTGGGAAAGTGCATATATACAGTTACAATTGGCAGTAATGACTACATTAACAACTACTTTATGCCAAGGTTATACTCAACTAGCCGCCAATTTACCTCTGAGCAATATGCAGGAGTTCTTATTCAACAGTATTCTGGGCAAATAAAAACGTTGTACAATTCTGGGGCAAGAAAGATAGCCCTTTTCGGGCTCGGTACAATTGGTTCTATACCCTTTGAAGTGGCTATGTGCAATCGCAGCACAAACAACGTCTCTTTATGCTCAGATAACATGAACAGTGCAGTCCAAGTTTTCAACGCAAGGCTTCAATCACTTGTCGATGACCTTAACAGCAACCTAACCGACGCTAAGTTCACTTATATAGATTTCTACGGGATTGGATTATCCAGTGCCGCTGCATCTCTTGGTTCTGTGATTTTTGATGCACCATGCTGTGAGGTGGTACGTGAGACCGGGCTATGCGTCCCCTTCAATACCCCATGCCAAAACAGGACTCAGTATTCCTTTTGGGATGCAATCCATCCTAGTGAGAGTTCAAATGTTGTTCTAGGCGGAAGAGCATACAATGCCACGCTTCCTACTGATGCTGTTCCATTTGATATTAATCAGCTAGCTCAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

40.38

Weight (kDa)

6.97

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 342 1.1e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 487
AciI CCGC 3 cut(s) 571, 879, 1038
AclI AACGTT 2 cut(s) 164, 635
AcuI CTGAAG 1 cut(s) 437
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 4 cut(s) 485, 641, 682, 930
AfiI CCNNNNNNNGG 2 cut(s) 487, 672
AgsI TTSAA 7 cut(s) 60, 611, 704, 781, 794, 958, 1023
AjnI CCWGG 2 cut(s) 381, 486
AluBI AGCT 3 cut(s) 476, 1096, 1100
AluI AGCT 3 cut(s) 476, 1096, 1100
Alw26I GTCTC 3 cut(s) 117, 742, 929
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 6 cut(s) 299, 302, 350, 473, 723, 881
AseI ATTAAT 1 cut(s) 1089
Asp700I GAANNNNTTC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 37, 212
AsuC2I CCSGG 1 cut(s) 940
AsuHPI GGTGA 2 cut(s) 413, 427
AsuNHI GCTAGC 1 cut(s) 1096
AvaII GGWCC 2 cut(s) 37, 212
BaeGI GKGCMC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 678
BbvI GCAGC 6 cut(s) 286, 289, 362, 485, 735, 868
BccI CCATC 3 cut(s) 80, 306, 1014
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 2 cut(s) 383, 488
BcnI CCSGG 1 cut(s) 940
BcoDI GTCTC 3 cut(s) 117, 742, 929
BfaI CTAG 5 cut(s) 269, 567, 1011, 1034, 1097
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 8 cut(s) 300, 303, 351, 474, 571, 724, 879, 882
BlsI GCNGC 8 cut(s) 301, 304, 352, 475, 572, 725, 880, 883
Bme1390I CCNGG 3 cut(s) 383, 488, 940
Bme18I GGWCC 2 cut(s) 37, 212
BmgT120I GGNCC 2 cut(s) 37, 212
BmiI GGNNCC 3 cut(s) 39, 93, 380
BmrFI CCNGG 3 cut(s) 383, 488, 940
BmsI GCATC 5 cut(s) 350, 893, 898, 988, 1060
BmtI GCTAGC 1 cut(s) 1100
BpuEI CTTGAG 1 cut(s) 1086
BpuMI CCSGG 1 cut(s) 940
BsaAI YACGTR 2 cut(s) 7, 932
BsaBI GATNNNNATC 1 cut(s) 1089
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 4 cut(s) 325, 382, 487, 551
BsaWI WCCGGW 2 cut(s) 218, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 672
Bse1I ACTGG 2 cut(s) 481, 873
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 2 cut(s) 383, 488
BseDI CCNNGG 4 cut(s) 325, 382, 487, 551
BseGI GGATG 4 cut(s) 317, 341, 1003, 1006
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 2 cut(s) 487, 672
BseMII CTCAG 3 cut(s) 576, 762, 995
BseNI ACTGG 2 cut(s) 481, 873
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 6 cut(s) 286, 289, 362, 485, 735, 868
BsgI GTGCAG 2 cut(s) 369, 786
BshFI GGCC 1 cut(s) 156
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 3 cut(s) 219, 345, 939
BslFI GGGAC 3 cut(s) 23, 387, 935
BslI CCNNNNNNNGG 2 cut(s) 487, 672
BsmAI GTCTC 3 cut(s) 117, 742, 929
BsmBI CGTCTC 1 cut(s) 742
BsmFI GGGAC 3 cut(s) 23, 387, 935
BsnI GGCC 1 cut(s) 156
Bso31I GGTCTC 1 cut(s) 929
Bsp1286I GDGCHC 2 cut(s) 94, 678
Bsp1407I TGTACA 1 cut(s) 639
BspACI CCGC 3 cut(s) 571, 879, 1038
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 3 cut(s) 577, 761, 994
BspLI GGNNCC 3 cut(s) 39, 93, 380
BspMI ACCTGC 1 cut(s) 344
BspOI GCTAGC 1 cut(s) 1100
BspQI GCTCTTC 1 cut(s) 1036
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 929
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 2 cut(s) 481, 873
BssECI CCNNGG 4 cut(s) 325, 382, 487, 551
BssT1I CCWWGG 1 cut(s) 551
Bst2UI CCWGG 2 cut(s) 383, 488
Bst4CI ACNGT 5 cut(s) 140, 329, 508, 615, 764
Bst6I CTCTTC 1 cut(s) 1036
BstAPI GCANNNNNTGC 2 cut(s) 347, 1052
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 2 cut(s) 7, 932
BstC8I GCNNGC 1 cut(s) 1098
BstDEI CTNAG 4 cut(s) 585, 748, 834, 981
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 4 cut(s) 317, 341, 1003, 1006
BstMAI GTCTC 3 cut(s) 117, 742, 929
BstMWI GCNNNNNNNGC 3 cut(s) 347, 723, 1052
BstNI CCWGG 2 cut(s) 383, 488
BstSCI CCNGG 3 cut(s) 381, 486, 938
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 6 cut(s) 286, 289, 362, 485, 735, 868
BstXI CCANNNNNNTGG 1 cut(s) 30
BsuRI GGCC 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 4 cut(s) 317, 341, 1003, 1006
BtsIMutI CAGTG 3 cut(s) 145, 769, 880
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 1098
CaiI CAGNNNCTG 1 cut(s) 487
Cfr13I GGNCC 2 cut(s) 37, 212
CseI GACGC 2 cut(s) 839, 937
Csp6I GTAC 4 cut(s) 484, 640, 681, 929
CviAII CATG 4 cut(s) 105, 757, 915, 966
CviQI GTAC 4 cut(s) 484, 640, 681, 929
DdeI CTNAG 4 cut(s) 585, 748, 834, 981
DraIII CACNNNGTG 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 1036
EarI CTCTTC 1 cut(s) 1036
EciI GGCGGA 1 cut(s) 1053
Eco130I CCWWGG 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 929
Eco47I GGWCC 2 cut(s) 37, 212
Eco57I CTGAAG 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 381, 486
EcoT14I CCWWGG 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 551
Esp3I CGTCTC 1 cut(s) 742
FaeI CATG 4 cut(s) 108, 760, 918, 969
FaqI GGGAC 3 cut(s) 23, 387, 935
FatI CATG 4 cut(s) 104, 756, 914, 965
Fnu4HI GCNGC 8 cut(s) 300, 303, 351, 474, 571, 724, 879, 882
FokI GGATG 4 cut(s) 324, 328, 993, 1010
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 8 cut(s) 300, 303, 351, 474, 571, 724, 879, 882
FspBI CTAG 5 cut(s) 269, 567, 1011, 1034, 1097
GluI GCNGC 8 cut(s) 300, 303, 351, 474, 571, 724, 879, 882
HaeIII GGCC 1 cut(s) 156
HapII CCGG 3 cut(s) 219, 345, 939
HgaI GACGC 2 cut(s) 839, 937
Hin1II CATG 4 cut(s) 108, 760, 918, 969
HinfI GANTC 4 cut(s) 125, 368, 418, 979
HpaII CCGG 3 cut(s) 219, 345, 939
HphI GGTGA 2 cut(s) 413, 427
Hpy166II GTNNAC 1 cut(s) 840
Hpy188I TCNGA 3 cut(s) 136, 586, 751
Hpy188III TCNNGA 2 cut(s) 362, 372
Hpy8I GTNNAC 1 cut(s) 840
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 3 cut(s) 216, 461, 964
HpyCH4III ACNGT 5 cut(s) 140, 329, 508, 615, 764
HpyCH4IV ACGT 6 cut(s) 6, 164, 238, 635, 735, 931
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 723, 1052
HpyF3I CTNAG 4 cut(s) 585, 748, 834, 981
HpySE526I ACGT 6 cut(s) 6, 164, 238, 635, 735, 931
Hsp92II CATG 4 cut(s) 108, 760, 918, 969
LguI GCTCTTC 1 cut(s) 1036
Lsp1109I GCAGC 6 cut(s) 286, 289, 362, 485, 735, 868
LweI GCATC 5 cut(s) 350, 893, 898, 988, 1060
MaeI CTAG 5 cut(s) 269, 567, 1011, 1034, 1097
MaeII ACGT 6 cut(s) 6, 164, 238, 635, 735, 931
MaeIII GTNAC 3 cut(s) 107, 394, 508
MboII GAAGA 1 cut(s) 1053
MfeI CAATTG 2 cut(s) 513, 684
MhlI GDGCHC 2 cut(s) 94, 678
MlyI GAGTC 2 cut(s) 119, 973
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 3 cut(s) 203, 592, 916
MroXI GAANNNNTTC 1 cut(s) 226
MseI TTAA 5 cut(s) 161, 402, 534, 813, 1089
MslI CAYNNNNRTG 2 cut(s) 28, 1011
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 3 cut(s) 219, 345, 939
MspR9I CCNGG 3 cut(s) 383, 488, 940
MunI CAATTG 2 cut(s) 513, 684
MvaI CCWGG 2 cut(s) 383, 488
MwoI GCNNNNNNNGC 3 cut(s) 347, 723, 1052
NciI CCSGG 1 cut(s) 940
NheI GCTAGC 1 cut(s) 1096
NlaIII CATG 4 cut(s) 108, 760, 918, 969
NlaIV GGNNCC 3 cut(s) 39, 93, 380
PciSI GCTCTTC 1 cut(s) 1036
PdmI GAANNNNTTC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 368, 418
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 8 cut(s) 301, 304, 352, 475, 572, 725, 880, 883
PleI GAGTC 2 cut(s) 119, 973
PpsI GAGTC 2 cut(s) 119, 973
Ppu21I YACGTR 2 cut(s) 7, 932
PshBI ATTAAT 1 cut(s) 1089
PsiI TTATAA 1 cut(s) 183
Psp1406I AACGTT 2 cut(s) 164, 635
Psp6I CCWGG 2 cut(s) 381, 486
PspGI CCWGG 2 cut(s) 381, 486
PspN4I GGNNCC 3 cut(s) 39, 93, 380
PspPI GGNCC 2 cut(s) 37, 212
PsrI GAACNNNNNNTAC 2 cut(s) 673, 705
PstNI CAGNNNCTG 1 cut(s) 487
RsaI GTAC 4 cut(s) 485, 641, 682, 930
RsaNI GTAC 4 cut(s) 484, 640, 681, 929
RseI CAYNNNNRTG 2 cut(s) 28, 1011
SapI GCTCTTC 1 cut(s) 1036
SaqAI TTAA 5 cut(s) 161, 402, 534, 813, 1089
SatI GCNGC 8 cut(s) 300, 303, 351, 474, 571, 724, 879, 882
Sau96I GGNCC 2 cut(s) 37, 212
SchI GAGTC 2 cut(s) 119, 973
ScrFI CCNGG 3 cut(s) 383, 488, 940
SduI GDGCHC 2 cut(s) 94, 678
SfaNI GCATC 5 cut(s) 350, 893, 898, 988, 1060
SinI GGWCC 2 cut(s) 37, 212
SmiMI CAYNNNNRTG 2 cut(s) 28, 1011
SmlI CTYRAG 1 cut(s) 1101
SmoI CTYRAG 1 cut(s) 1101
SsiI CCGC 3 cut(s) 571, 879, 1038
SspMI CTAG 5 cut(s) 269, 567, 1011, 1034, 1097
StyD4I CCNGG 3 cut(s) 381, 486, 938
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 5 cut(s) 140, 329, 508, 615, 764
TaiI ACGT 6 cut(s) 9, 167, 241, 638, 738, 934
TaqI TCGA 1 cut(s) 804
TatI WGTACW 1 cut(s) 639
TauI GCSGC 2 cut(s) 573, 881
TfiI GAWTC 2 cut(s) 368, 418
Tru1I TTAA 5 cut(s) 161, 402, 534, 813, 1089
Tru9I TTAA 5 cut(s) 161, 402, 534, 813, 1089
TscAI CASTG 3 cut(s) 145, 769, 880
TseI GCWGC 6 cut(s) 299, 302, 350, 473, 723, 881
TspDTI ATGAA 4 cut(s) 32, 103, 381, 773
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 3 cut(s) 145, 769, 880
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 37, 212
VspI ATTAAT 1 cut(s) 1089
XmnI GAANNNNTTC 1 cut(s) 226
XspI CTAG 5 cut(s) 269, 567, 1011, 1034, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.