Rroxscaffold_5G00345560

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014711
Physical Location & Seq
Reverse (-)
15986683 .. 15988417
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345560.1

Sequence Viewer

Length: 1110 bp
ATGGCCGCCACACTTCGTGAAGTAGCTATAAAGGCATTGTGGGTGTACTTTTCTGTTTTGTTGTTGTTCGTTTCATCGAATATGCAGTACTTGGTTGATGGGGCATCACAAGTTCCTTGTTACTTCATATTTGGAGACTCGTTATCCGATAGTGGAAATAACAATGGGCTTTTGACACTGGCCAAAGTCAATTATCGTCCCTATGGCATTGACTTTCCACAAGGTCCAACTGGAAGGTTCTCCAACGGACGTAACTTGGTCGATATTATTGCTGAACTACTAGGTTTTGATGAATATATACCCACTTTCGCGGCTGCAATTGGAGGAGATATAACAAAAGGTGTAAATTATGCATCTGGTGCAGCTGGTATCCGTGACGAATCTGGACGTAACCAGGGAATGAGAATAACCTTGTATAAGCAATTGATAAATCACCAAATCATAGTCAATAAGCTTGTTGCCAATGGTTCACTCGCTGCAGATTATCTAAGCAAGTGCATATATTCGATTGGGATAGGCACTAACGATTACATTAACAACTACTTCATGCCAAAACTCTACCCAACTAGCCGCTTATATACTCCAAAGCAATATGCAGATGTTCTTGTCGAAGAATACTCTCAGCAATTACGGACTCTGTACAACTCGGGGGCAAGGAAGATGGCCCTTATTGGACTAGGTACGATAGGTTCCACACCCTTCGAAGTGGCAATGTGTGGCATAAACGGCACCGTATGTGTTGATAACATCAACTATGCTGCCCAACTTTTCAATGCAAGGCTTAAATCACTTGTCACTGATCTTAACACAAATCTAACAAATGCTAAGTTCATTTACATAGATTATTATGGAATTGGACTATCCAGTGCTGCTTCATCTCCTCCTGGTTCTGTTAATTACACTGCACCATGCTGTGAAGTTGAAATGAATAGTACGACGGGGCTATGCACCCCGTTCAAGACCCCATGCCAGAATAGAACTCAATATGCATTTTGGGATGCTATCCATCCTACTGAGATTTCAAATGTGGTTATAGCAGCAAGAGCATATAATGCTACGCTACCTACTGATGCTTTTCCATATGATATTAGTCACTTAGCTCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.3

Weight (kDa)

5.94

Isoelectric Point (pI)

22.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 347 3.2e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 728
AccII CGCG 1 cut(s) 311
AciI CCGC 3 cut(s) 6, 311, 571
AcoI YGGCCR 2 cut(s) 3, 180
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 5 cut(s) 47, 89, 641, 682, 934
AgsI TTSAA 4 cut(s) 772, 923, 958, 1023
AjnI CCWGG 2 cut(s) 393, 883
AluBI AGCT 4 cut(s) 26, 365, 454, 1100
AluI AGCT 4 cut(s) 26, 365, 454, 1100
Alw26I GTCTC 1 cut(s) 129
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 3 cut(s) 3, 180, 663
ApeKI GCWGC 6 cut(s) 314, 362, 476, 758, 869, 1037
AspS9I GGNCC 2 cut(s) 224, 664
AsuHPI GGTGA 1 cut(s) 425
AsuII TTCGAA 1 cut(s) 702
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 728
BbvI GCAGC 6 cut(s) 301, 374, 463, 745, 856, 1049
BccI CCATC 3 cut(s) 92, 655, 1014
BceAI ACGGC 1 cut(s) 742
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 395, 885
BciVI GTATCC 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 3 cut(s) 281, 567, 677
BfmI CTRYAG 1 cut(s) 477
BfuI GTATCC 1 cut(s) 380
BisI GCNGC 9 cut(s) 6, 312, 315, 363, 477, 571, 759, 870, 1038
BlsI GCNGC 9 cut(s) 7, 313, 316, 364, 478, 572, 760, 871, 1039
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 395, 885
Bme18I GGWCC 1 cut(s) 224
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 224, 664
BmiI GGNNCC 2 cut(s) 691, 730
BmrFI CCNGG 2 cut(s) 395, 885
BmsI GCATC 4 cut(s) 113, 362, 988, 1060
Bpu14I TTCGAA 1 cut(s) 702
BsaJI CCNNGG 1 cut(s) 394
Bse1I ACTGG 3 cut(s) 183, 235, 864
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 2 cut(s) 395, 885
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 1003, 1006
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 635, 1005
BseNI ACTGG 3 cut(s) 183, 235, 864
BseRI GAGGAG 2 cut(s) 339, 870
BseXI GCAGC 6 cut(s) 301, 374, 463, 745, 856, 1049
BsgI GTGCAG 2 cut(s) 381, 888
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 5, 182, 665
BshNI GGYRCC 1 cut(s) 728
BsiHKCI CYCGRG 1 cut(s) 646
BslFI GGGAC 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 3 cut(s) 5, 182, 665
BsoBI CYCGRG 1 cut(s) 646
Bsp119I TTCGAA 1 cut(s) 702
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 1 cut(s) 799
BspACI CCGC 3 cut(s) 6, 311, 571
BspANI GGCC 3 cut(s) 5, 182, 665
BspCNI CTCAG 2 cut(s) 634, 1006
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 691, 730
BspMAI CTGCAG 1 cut(s) 481
BspT104I TTCGAA 1 cut(s) 702
BspT107I GGYRCC 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 717
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 3 cut(s) 183, 235, 864
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 799
Bst2UI CCWGG 2 cut(s) 395, 885
Bst4CI ACNGT 1 cut(s) 733
BstAPI GCANNNNNTGC 2 cut(s) 359, 1052
BstAUI TGTACA 1 cut(s) 639
BstBI TTCGAA 1 cut(s) 702
BstDEI CTNAG 5 cut(s) 488, 621, 825, 1014, 1096
BstF5I GGATG 2 cut(s) 1003, 1006
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 1 cut(s) 802
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 1 cut(s) 799
BstMWI GCNNNNNNNGC 5 cut(s) 32, 359, 726, 1043, 1052
BstNI CCWGG 2 cut(s) 395, 885
BstSCI CCNGG 2 cut(s) 393, 883
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 6 cut(s) 301, 374, 463, 745, 856, 1049
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 3 cut(s) 5, 182, 665
BtsCI GGATG 2 cut(s) 1003, 1006
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 4 cut(s) 176, 795, 871, 900
Cfr13I GGNCC 2 cut(s) 224, 664
Csp6I GTAC 5 cut(s) 46, 88, 640, 681, 933
CviAII CATG 3 cut(s) 547, 909, 966
CviQI GTAC 5 cut(s) 46, 88, 640, 681, 933
DdeI CTNAG 5 cut(s) 488, 621, 825, 1014, 1096
DpnI GATC 1 cut(s) 801
DpnII GATC 1 cut(s) 799
DraIII CACNNNGTG 1 cut(s) 17
EaeI YGGCCR 2 cut(s) 3, 180
Eco47I GGWCC 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 393, 883
EcoT22I ATGCAT 2 cut(s) 355, 991
FaeI CATG 3 cut(s) 550, 912, 969
FaqI GGGAC 1 cut(s) 183
FatI CATG 3 cut(s) 546, 908, 965
FauNDI CATATG 1 cut(s) 1081
Fnu4HI GCNGC 9 cut(s) 6, 312, 315, 363, 477, 571, 759, 870, 1038
FokI GGATG 2 cut(s) 993, 1010
Fsp4HI GCNGC 9 cut(s) 6, 312, 315, 363, 477, 571, 759, 870, 1038
FspBI CTAG 3 cut(s) 281, 567, 677
GluI GCNGC 9 cut(s) 6, 312, 315, 363, 477, 571, 759, 870, 1038
HaeIII GGCC 3 cut(s) 5, 182, 665
Hin1II CATG 3 cut(s) 550, 912, 969
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 137, 380, 634
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 46, 470
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 17, 384, 958
Hpy8I GTNNAC 2 cut(s) 46, 470
Hpy99I CGWCG 1 cut(s) 940
HpyAV CCTTC 2 cut(s) 228, 709
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 2 cut(s) 250, 388
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 359, 726, 1043, 1052
HpyF3I CTNAG 5 cut(s) 488, 621, 825, 1014, 1096
HpySE526I ACGT 2 cut(s) 250, 388
Hsp92II CATG 3 cut(s) 550, 912, 969
Kzo9I GATC 1 cut(s) 799
Lsp1109I GCAGC 6 cut(s) 301, 374, 463, 745, 856, 1049
LweI GCATC 4 cut(s) 113, 362, 988, 1060
MaeI CTAG 3 cut(s) 281, 567, 677
MaeII ACGT 2 cut(s) 250, 388
MaeIII GTNAC 6 cut(s) 119, 251, 374, 389, 793, 1091
MalI GATC 1 cut(s) 801
MboI GATC 1 cut(s) 799
MboII GAAGA 2 cut(s) 623, 670
MfeI CAATTG 2 cut(s) 318, 422
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 7 cut(s) 190, 318, 346, 422, 626, 852, 895
MluNI TGGCCA 1 cut(s) 182
MlyI GAGTC 2 cut(s) 131, 628
MmeI TCCRAC 2 cut(s) 251, 267
MnlI CCTC 2 cut(s) 317, 891
Mox20I TGGCCA 1 cut(s) 182
Mph1103I ATGCAT 2 cut(s) 355, 991
MscI TGGCCA 1 cut(s) 182
MseI TTAA 4 cut(s) 534, 783, 804, 894
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 365
MspR9I CCNGG 2 cut(s) 395, 885
MunI CAATTG 2 cut(s) 318, 422
MvaI CCWGG 2 cut(s) 395, 885
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 5 cut(s) 32, 359, 726, 1043, 1052
NdeI CATATG 1 cut(s) 1081
NdeII GATC 1 cut(s) 799
NlaIII CATG 3 cut(s) 550, 912, 969
NlaIV GGNNCC 2 cut(s) 691, 730
NmuCI GTSAC 3 cut(s) 374, 793, 1091
NsiI ATGCAT 2 cut(s) 355, 991
NspV TTCGAA 1 cut(s) 702
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 9 cut(s) 7, 313, 316, 364, 478, 572, 760, 871, 1039
PleI GAGTC 2 cut(s) 131, 628
PpsI GAGTC 2 cut(s) 131, 628
Psp6I CCWGG 2 cut(s) 393, 883
PspGI CCWGG 2 cut(s) 393, 883
PspN4I GGNNCC 2 cut(s) 691, 730
PspPI GGNCC 2 cut(s) 224, 664
PsrI GAACNNNNNNTAC 2 cut(s) 673, 705
PstI CTGCAG 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 5 cut(s) 47, 89, 641, 682, 934
RsaNI GTAC 5 cut(s) 46, 88, 640, 681, 933
SaqAI TTAA 4 cut(s) 534, 783, 804, 894
SatI GCNGC 9 cut(s) 6, 312, 315, 363, 477, 571, 759, 870, 1038
Sau3AI GATC 1 cut(s) 799
Sau96I GGNCC 2 cut(s) 224, 664
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 2 cut(s) 131, 628
ScrFI CCNGG 2 cut(s) 395, 885
SfaNI GCATC 4 cut(s) 113, 362, 988, 1060
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 702
SinI GGWCC 1 cut(s) 224
Sse9I AATT 7 cut(s) 190, 318, 346, 422, 626, 852, 895
SsiI CCGC 3 cut(s) 6, 311, 571
SspMI CTAG 3 cut(s) 281, 567, 677
StyD4I CCNGG 2 cut(s) 393, 883
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 2 cut(s) 253, 391
TaqI TCGA 5 cut(s) 77, 261, 506, 609, 702
TasI AATT 7 cut(s) 190, 318, 346, 422, 626, 852, 895
TatI WGTACW 3 cut(s) 45, 87, 639
TauI GCSGC 3 cut(s) 8, 314, 573
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 4 cut(s) 534, 783, 804, 894
Tru9I TTAA 4 cut(s) 534, 783, 804, 894
TscAI CASTG 4 cut(s) 183, 802, 871, 907
TseFI GTSAC 3 cut(s) 374, 793, 1091
TseI GCWGC 6 cut(s) 314, 362, 476, 758, 869, 1037
Tsp45I GTSAC 3 cut(s) 374, 793, 1091
TspDTI ATGAA 7 cut(s) 63, 115, 306, 535, 820, 864, 941
TspGWI ACGGA 3 cut(s) 261, 362, 646
TspRI CASTG 4 cut(s) 183, 802, 871, 907
VpaK11BI GGWCC 1 cut(s) 224
XspI CTAG 3 cut(s) 281, 567, 677
ZrmI AGTACT 1 cut(s) 89
Zsp2I ATGCAT 2 cut(s) 355, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.