Rorug04G0025400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014711
Physical Location & Seq
Forward (+)
3489900 .. 3498345
8446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0025400.1

Sequence Viewer

Length: 1737 bp
ATGTCTCACTCCCTCCACCTCCTCACCCCCACCACCTTAACCTTCCCCAAACCCCATTCCTCCTCCTCATTCCTCAACCCCTTCTCCACTCCCTATCGCCGGTTTCCGGTCACCACCGCCTTCCCCACCCTCTCCATCAAAGCCTCCGCCGCCTCATCGGATCCCCAACCCTCCGTCCTCGTCTCCGGCAACGGCACCTCCTCCTCCGCGGTACAATCCCAGCAGGACTATGCCTCCTCCACCTCCATCGAGGTCGATGCCGTTACGGAAGCCGAGCTCCGGGAGAACGGCTTCCGAAGCACGCGGCGGACGAAGCTCGTCTGCACCATCGGCCCCGCCACCGCCGGCTTCGACCAGCTCGAGGCCCTCGCCGTCGGCGGCATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCGGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACCGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCCGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCGTTCGGTTCTACTCTTCCGGAACGCACCATCAATGTGAATTACGAGGGTTTCGCCGAAGATGTGAAAGTGGGCGATGAGCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAAATGTACTGATCCTGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGAGAACGAAATTCCATGCTTCCCACAATTTCTTCCAAGGATTGGTTGGACATTGATTTCGGGATTGCTGAGGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCATCTTAAAAGCTATATTGCAGCACGGTCTAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAGGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATCGTCGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCCGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCAGAGGAGCTTTGTATTTCTGCTGCCAAGATGGCAAATAATTTAGAGGTGGATGCTCTTTTCATCTTTACAAAGACGGGCCACATGGCATCTCTACTGTCACGCTGCCGGCCTGACTGCCCAATCTTTGCTTTTACCAACACAACATCTGTGCGGAGACGTCTGAACCTACAGTGGGGGCTGATACCATTTCGTGTTAGTTTCTCTGATGATATGGAAAGCAACCTTCACAAAACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

578

Amino Acids

63.42

Weight (kDa)

5.63

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 102 - 430 2.5e-90 Pyruvate kinase, barrel domain
PK_C PF02887 469 - 567 2.3e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1564
AccB1I GGYRCC 2 cut(s) 194, 426
AccB7I CCANNNNNTGG 2 cut(s) 843, 1376
AccII CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
AccIII TCCGGA 1 cut(s) 604
AclWI GGATC 5 cut(s) 155, 168, 737, 1328, 1387
AcoI YGGCCR 1 cut(s) 1203
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 4 cut(s) 933, 1218, 1236, 1670
AcyI GRCGYC 1 cut(s) 1561
AfaI GTAC 3 cut(s) 213, 739, 1731
AfiI CCNNNNNNNGG 8 cut(s) 99, 106, 279, 361, 843, 1101, 1376, 1576
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 2 cut(s) 1112, 1718
AjnI CCWGG 3 cut(s) 392, 745, 1655
Alw21I GWGCWC 2 cut(s) 279, 1066
Alw26I GTCTC 4 cut(s) 9, 187, 1552, 1699
AlwI GGATC 5 cut(s) 155, 168, 737, 1328, 1387
Ama87I CYCGRG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 8 cut(s) 331, 363, 715, 1203, 1270, 1480, 1511, 1653
ApeKI GCWGC 6 cut(s) 757, 944, 1123, 1228, 1424, 1506
ApoI RAATTY 1 cut(s) 811
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
Asp700I GAANNNNTTC 1 cut(s) 290
AspA2I CCTAGG 1 cut(s) 1057
AspLEI GCGC 3 cut(s) 420, 434, 447
AspS9I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
AsuC2I CCSGG 2 cut(s) 281, 719
AsuHPI GGTGA 8 cut(s) 16, 103, 448, 572, 700, 1064, 1268, 1688
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 1084
AvrII CCTAGG 1 cut(s) 1057
BamHI GGATCC 1 cut(s) 160
BanI GGYRCC 2 cut(s) 194, 426
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 279, 1066
BbvCI CCTCAGC 1 cut(s) 870
BbvI GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BceAI ACGGC 7 cut(s) 208, 245, 304, 356, 427, 464, 1205
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 370, 404
BciT130I CCWGG 3 cut(s) 394, 747, 1657
BciVI GTATCC 2 cut(s) 490, 1188
BclI TGATCA 2 cut(s) 918, 1665
BcnI CCSGG 2 cut(s) 281, 719
BcoDI GTCTC 4 cut(s) 9, 187, 1552, 1699
BfaI CTAG 3 cut(s) 954, 1058, 1191
BfmI CTRYAG 1 cut(s) 1571
BfuI GTATCC 2 cut(s) 490, 1188
BglI GCCNNNNNGGC 1 cut(s) 1433
BlnI CCTAGG 1 cut(s) 1057
Bme1390I CCNGG 5 cut(s) 281, 394, 719, 747, 1657
Bme18I GGWCC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
BmiI GGNNCC 5 cut(s) 162, 196, 334, 428, 1086
BmrFI CCNGG 5 cut(s) 281, 394, 719, 747, 1657
BmsI GCATC 6 cut(s) 247, 1034, 1228, 1236, 1444, 1499
BpmI CTGGAG 1 cut(s) 802
Bpu10I CCTNAGC 1 cut(s) 870
BpuMI CCSGG 2 cut(s) 281, 719
BsaHI GRCGYC 1 cut(s) 1561
BsaJI CCNNGG 6 cut(s) 207, 409, 552, 761, 837, 1057
BsaWI WCCGGW 2 cut(s) 106, 604
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 182, 212, 218, 248, 1026, 1056
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 106, 279, 361, 843, 1101, 1376, 1576
Bse118I RCCGGY 3 cut(s) 99, 344, 1509
Bse1I ACTGG 3 cut(s) 428, 1136, 1273
Bse3DI GCAATG 3 cut(s) 885, 1044, 1686
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 3 cut(s) 394, 747, 1657
BseDI CCNNGG 6 cut(s) 207, 409, 552, 761, 837, 1057
BseGI GGATG 6 cut(s) 562, 682, 727, 793, 1017, 1459
BseLI CCNNNNNNNGG 8 cut(s) 99, 106, 279, 361, 843, 1101, 1376, 1576
BseMI GCAATG 3 cut(s) 885, 1044, 1686
BseMII CTCAG 4 cut(s) 861, 1079, 1315, 1710
BseNI ACTGG 3 cut(s) 428, 1136, 1273
BseRI GAGGAG 7 cut(s) 11, 52, 55, 190, 193, 226, 1421
BseXI GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BseYI CCCAGC 3 cut(s) 219, 1095, 1369
BsgI GTGCAG 1 cut(s) 307
Bsh1236I CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
BshFI GGCC 8 cut(s) 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BshNI GGYRCC 2 cut(s) 194, 426
BsiHKAI GWGCWC 2 cut(s) 279, 1066
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 8 cut(s) 100, 107, 186, 280, 345, 605, 719, 1510
BslFI GGGAC 1 cut(s) 1070
BslI CCNNNNNNNGG 8 cut(s) 99, 106, 279, 361, 843, 1101, 1376, 1576
BsmAI GTCTC 4 cut(s) 9, 187, 1552, 1699
BsmBI CGTCTC 2 cut(s) 187, 1552
BsmFI GGGAC 1 cut(s) 1070
BsnI GGCC 8 cut(s) 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 279, 1066
Bsp13I TCCGGA 1 cut(s) 604
Bsp143I GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
Bsp68I TCGCGA 1 cut(s) 485
BspANI GGCC 8 cut(s) 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BspCNI CTCAG 4 cut(s) 862, 1078, 1314, 1709
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 5 cut(s) 162, 196, 334, 428, 1086
BspPI GGATC 5 cut(s) 155, 168, 737, 1328, 1387
BspT107I GGYRCC 2 cut(s) 194, 426
BsrDI GCAATG 3 cut(s) 885, 1044, 1686
BsrFI RCCGGY 3 cut(s) 99, 344, 1509
BsrI ACTGG 3 cut(s) 428, 1136, 1273
BssAI RCCGGY 3 cut(s) 99, 344, 1509
BssECI CCNNGG 6 cut(s) 207, 409, 552, 761, 837, 1057
BssMI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
BssNI GRCGYC 1 cut(s) 1561
BssT1I CCWWGG 2 cut(s) 837, 1057
Bst2UI CCWGG 3 cut(s) 394, 747, 1657
Bst4CI ACNGT 5 cut(s) 753, 951, 1397, 1500, 1575
Bst6I CTCTTC 3 cut(s) 606, 1005, 1335
BstACI GRCGYC 1 cut(s) 1561
BstC8I GCNNGC 6 cut(s) 302, 346, 416, 765, 1233, 1511
BstDEI CTNAG 4 cut(s) 870, 1065, 1301, 1696
BstDSI CCRYGG 3 cut(s) 207, 409, 761
BstEII GGTNACC 3 cut(s) 109, 1052, 1676
BstF5I GGATG 6 cut(s) 562, 682, 727, 793, 1017, 1459
BstFNI CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
BstHHI GCGC 3 cut(s) 420, 434, 447
BstKTI GATC 9 cut(s) 163, 529, 745, 921, 1017, 1173, 1323, 1382, 1668
BstMAI GTCTC 4 cut(s) 9, 187, 1552, 1699
BstMBI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
BstNI CCWGG 3 cut(s) 394, 747, 1657
BstNSI RCATGY 1 cut(s) 406
BstPI GGTNACC 3 cut(s) 109, 1052, 1676
BstSCI CCNGG 5 cut(s) 279, 392, 717, 745, 1655
BstSFI CTRYAG 1 cut(s) 1571
BstUI CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
BstV1I GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuI GTATCC 2 cut(s) 490, 1188
BsuRI GGCC 8 cut(s) 333, 365, 717, 1205, 1272, 1482, 1513, 1655
BtgI CCRYGG 3 cut(s) 207, 409, 761
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 6 cut(s) 562, 682, 727, 793, 1017, 1459
BtsI GCAGTG 1 cut(s) 1643
BtsIMutI CAGTG 3 cut(s) 583, 1580, 1643
BtuMI TCGCGA 1 cut(s) 485
Cac8I GCNNGC 6 cut(s) 302, 346, 416, 765, 1233, 1511
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 3 cut(s) 420, 434, 447
Cfr10I RCCGGY 3 cut(s) 99, 344, 1509
Cfr13I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
Cfr42I CCGCGG 2 cut(s) 210, 764
Csp6I GTAC 3 cut(s) 212, 738, 1730
CviAII CATG 7 cut(s) 382, 403, 520, 817, 1352, 1486, 1734
CviQI GTAC 3 cut(s) 212, 738, 1730
DdeI CTNAG 4 cut(s) 870, 1065, 1301, 1696
DpnI GATC 9 cut(s) 162, 528, 744, 920, 1016, 1172, 1322, 1381, 1667
DpnII GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
EaeI YGGCCR 1 cut(s) 1203
Eam1104I CTCTTC 3 cut(s) 606, 1005, 1335
EarI CTCTTC 3 cut(s) 606, 1005, 1335
EciI GGCGGA 2 cut(s) 136, 322
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 837, 1057
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 1084
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 4 cut(s) 933, 1218, 1236, 1670
Eco88I CYCGRG 1 cut(s) 359
Eco91I GGTNACC 3 cut(s) 109, 1052, 1676
EcoICRI GAGCTC 1 cut(s) 277
EcoO109I RGGNCCY 2 cut(s) 364, 1084
EcoO65I GGTNACC 3 cut(s) 109, 1052, 1676
EcoRII CCWGG 3 cut(s) 392, 745, 1655
EcoT14I CCWWGG 2 cut(s) 837, 1057
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 2 cut(s) 837, 1057
Esp3I CGTCTC 2 cut(s) 187, 1552
FaeI CATG 7 cut(s) 385, 406, 523, 820, 1355, 1489, 1737
FaqI GGGAC 1 cut(s) 1070
FatI CATG 7 cut(s) 381, 402, 519, 816, 1351, 1485, 1733
FauI CCCGC 2 cut(s) 343, 756
FbaI TGATCA 2 cut(s) 918, 1665
FokI GGATG 6 cut(s) 569, 689, 734, 800, 1004, 1466
FriOI GRGCYC 1 cut(s) 279
FspBI CTAG 3 cut(s) 954, 1058, 1191
GlaI GCGC 3 cut(s) 419, 433, 446
GsaI CCCAGC 3 cut(s) 223, 1099, 1373
GsuI CTGGAG 1 cut(s) 802
HaeIII GGCC 8 cut(s) 333, 365, 717, 1205, 1272, 1482, 1513, 1655
HapII CCGG 8 cut(s) 100, 107, 186, 280, 345, 605, 719, 1510
HhaI GCGC 3 cut(s) 420, 434, 447
Hin1I GRCGYC 1 cut(s) 1561
Hin1II CATG 7 cut(s) 385, 406, 523, 820, 1355, 1489, 1737
Hin6I GCGC 3 cut(s) 418, 432, 445
HinP1I GCGC 3 cut(s) 418, 432, 445
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 5 cut(s) 514, 992, 1163, 1259, 1660
HpaII CCGG 8 cut(s) 100, 107, 186, 280, 345, 605, 719, 1510
HphI GGTGA 8 cut(s) 16, 103, 448, 572, 700, 1064, 1268, 1688
Hpy188III TCNNGA 6 cut(s) 484, 605, 781, 862, 1324, 1352
Hpy99I CGWCG 4 cut(s) 377, 392, 485, 1148
HpyAV CCTTC 7 cut(s) 52, 91, 130, 497, 1637, 1645, 1648
HpyCH4III ACNGT 5 cut(s) 753, 951, 1397, 1500, 1575
HpyCH4IV ACGT 3 cut(s) 387, 1348, 1561
HpyCH4V TGCA 4 cut(s) 324, 944, 1025, 1708
HpyF3I CTNAG 4 cut(s) 870, 1065, 1301, 1696
HpySE526I ACGT 3 cut(s) 387, 1348, 1561
Hsp92I GRCGYC 1 cut(s) 1561
Hsp92II CATG 7 cut(s) 385, 406, 523, 820, 1355, 1489, 1737
HspAI GCGC 3 cut(s) 418, 432, 445
Kpn2I TCCGGA 1 cut(s) 604
KroI GCCGGC 2 cut(s) 344, 1509
KroNI GCCGGC 2 cut(s) 346, 1511
Ksp22I TGATCA 2 cut(s) 918, 1665
KspI CCGCGG 2 cut(s) 210, 764
Kzo9I GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
LmnI GCTCC 3 cut(s) 282, 1034, 1408
Lsp1109I GCAGC 6 cut(s) 744, 956, 1135, 1240, 1411, 1493
LweI GCATC 6 cut(s) 247, 1034, 1228, 1236, 1444, 1499
MaeI CTAG 3 cut(s) 954, 1058, 1191
MaeII ACGT 3 cut(s) 387, 1348, 1561
MaeIII GTNAC 6 cut(s) 109, 262, 1052, 1362, 1500, 1676
MalI GATC 9 cut(s) 162, 528, 744, 920, 1016, 1172, 1322, 1381, 1667
MboI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
MboII GAAGA 5 cut(s) 593, 656, 825, 1022, 1352
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 279, 1066
MluCI AATT 4 cut(s) 625, 811, 828, 1441
MlyI GAGTC 2 cut(s) 986, 1268
MmeI TCCRAC 1 cut(s) 828
MroI TCCGGA 1 cut(s) 604
MroNI GCCGGC 2 cut(s) 344, 1509
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 2 cut(s) 38, 930
MslI CAYNNNNRTG 1 cut(s) 620
MspA1I CMGCKG 2 cut(s) 209, 763
MspI CCGG 8 cut(s) 100, 107, 186, 280, 345, 605, 719, 1510
MspR9I CCNGG 5 cut(s) 281, 394, 719, 747, 1657
MvaI CCWGG 3 cut(s) 394, 747, 1657
MvnI CGCG 9 cut(s) 209, 304, 418, 420, 432, 434, 447, 485, 763
NaeI GCCGGC 2 cut(s) 346, 1511
NciI CCSGG 2 cut(s) 281, 719
NdeII GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
NgoMIV GCCGGC 2 cut(s) 344, 1509
NlaIII CATG 7 cut(s) 385, 406, 523, 820, 1355, 1489, 1737
NlaIV GGNNCC 5 cut(s) 162, 196, 334, 428, 1086
NmeAIII GCCGAG 3 cut(s) 298, 510, 577
NmuCI GTSAC 4 cut(s) 109, 1052, 1500, 1676
NruI TCGCGA 1 cut(s) 485
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 1351
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 3 cut(s) 308, 357, 417
PdiI GCCGGC 2 cut(s) 346, 1511
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 3 cut(s) 514, 1163, 1660
PflFI GACNNNGTC 1 cut(s) 1682
PflMI CCANNNNNTGG 2 cut(s) 843, 1376
PfoI TCCNGGA 2 cut(s) 279, 745
PleI GAGTC 2 cut(s) 986, 1267
PpsI GAGTC 2 cut(s) 986, 1267
PpuMI RGGWCCY 1 cut(s) 1084
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 3 cut(s) 392, 745, 1655
PspEI GGTNACC 3 cut(s) 109, 1052, 1676
PspFI CCCAGC 3 cut(s) 219, 1095, 1369
PspGI CCWGG 3 cut(s) 392, 745, 1655
PspN4I GGNNCC 5 cut(s) 162, 196, 334, 428, 1086
PspPI GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
PspPPI RGGWCCY 1 cut(s) 1084
PspXI VCTCGAGB 1 cut(s) 359
PsrI GAACNNNNNNTAC 2 cut(s) 1347, 1379
PsuI RGATCY 1 cut(s) 160
PsyI GACNNNGTC 1 cut(s) 1682
RruI TCGCGA 1 cut(s) 485
RsaI GTAC 3 cut(s) 213, 739, 1731
RsaNI GTAC 3 cut(s) 212, 738, 1730
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 279
SacII CCGCGG 2 cut(s) 210, 764
SaqAI TTAA 2 cut(s) 38, 930
Sau3AI GATC 9 cut(s) 160, 526, 742, 918, 1014, 1170, 1320, 1379, 1665
Sau96I GGNCC 6 cut(s) 332, 364, 716, 1084, 1270, 1480
SchI GAGTC 2 cut(s) 986, 1268
ScrFI CCNGG 5 cut(s) 281, 394, 719, 747, 1657
SduI GDGCHC 2 cut(s) 279, 1066
SfaNI GCATC 6 cut(s) 247, 1034, 1228, 1236, 1444, 1499
SfcI CTRYAG 1 cut(s) 1571
Sfr274I CTCGAG 1 cut(s) 359
Sfr303I CCGCGG 2 cut(s) 210, 764
SgrBI CCGCGG 2 cut(s) 210, 764
SinI GGWCC 1 cut(s) 1084
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 4 cut(s) 625, 811, 828, 1441
SspMI CTAG 3 cut(s) 954, 1058, 1191
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 5 cut(s) 279, 392, 717, 745, 1655
StyI CCWWGG 2 cut(s) 837, 1057
TaaI ACNGT 5 cut(s) 753, 951, 1397, 1500, 1575
TaiI ACGT 3 cut(s) 390, 1351, 1564
TaqI TCGA 7 cut(s) 249, 255, 351, 360, 1161, 1173, 1323
TasI AATT 4 cut(s) 625, 811, 828, 1441
TatI WGTACW 1 cut(s) 737
TauI GCSGC 4 cut(s) 152, 307, 381, 763
TfiI GAWTC 3 cut(s) 514, 1163, 1660
Tru1I TTAA 2 cut(s) 38, 930
Tru9I TTAA 2 cut(s) 38, 930
TscAI CASTG 3 cut(s) 583, 1580, 1650
TseFI GTSAC 4 cut(s) 109, 1052, 1500, 1676
TseI GCWGC 6 cut(s) 757, 944, 1123, 1228, 1424, 1506
Tsp45I GTSAC 4 cut(s) 109, 1052, 1500, 1676
TspDTI ATGAA 4 cut(s) 398, 874, 1368, 1453
TspGWI ACGGA 3 cut(s) 163, 281, 532
TspRI CASTG 3 cut(s) 583, 1580, 1650
Tth111I GACNNNGTC 1 cut(s) 1682
Van91I CCANNNNNTGG 2 cut(s) 843, 1376
VpaK11BI GGWCC 1 cut(s) 1084
XapI RAATTY 1 cut(s) 811
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XhoI CTCGAG 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 1057
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 3 cut(s) 954, 1058, 1191
ZraI GACGTC 1 cut(s) 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.