RchiOBHm_Chr4g0400521

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014711
Physical Location & Seq
Reverse (-)
18178274 .. 18180053
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37253

Sequence Viewer

Length: 1188 bp
ATGAACAGTAGATCCACATTTAGCATACGTAGAATAATTTGTACTTTTAGTTTGTTGTTGTTAAATTTGGGATCATCAATGGCCGCCACACTTCGTGAAGTAGCTATAAAGGCATTGTGGGTGTACTTTTCTGTTTTGTTGTTGTTCGTTTCATCGAATATGCATTACTTGGTTGATGGGGCATCACAAGTTCCTTGTTACTTCATATTTGGAGACTCGTTATCCGATAGTGGAAATAACAATGGGCTTTTGACACTGGCCGAAGTCAATTATCGTCCCTATGGCATTGACTTTCCACAAGGTCCAACTGGAAGGTTCTCCAACGGACGTAACTTGGTCGATATTATTGCTGAACTACTAGGTTTTGATGAATATATACCCACTTTCGCGGCTGCAATTGGAGGAGATATAACAAAAGGTGTAAATTATGCATCTGGTGCAGCTGGTATCCGTGACGAATCTGGACGTAACCAGGGAATGAGAATAACCTTGTATAAGCAATTGATAAATCACCAAATCATAGTCAATAAGCTTGTTGCCAATGGTTCACTCGCTGCAGATTATCTAAGCAAGTGCATATATTCGATTGGGATAGGCGCTAACGATTACATTAACAACTACTTCATGCCAAAACTCTACCCAACTAGCCGCTTATATACTCCAAAGCAATATGCAGATGTTCTTGTCCAAGAATACTCTCAGCAATTACGGACTCTGTACAACTCGGGGGCAAGGAAGATGGCCCTTATTGGACTAGGTATGATAGGTTCCACACCCTTCGAAGTGGCAATGTGTGGCACAAACGGCACCGTATGTGTTGATAACATCAACAATGCTGCCCAACTTTTCAATGCAAGGCTTAAATCACTTGTCACTGATCTTAACACAAATCTAACAAATGCTAAGTTCATTTACATAGATTATTATGGAATTGGACTATCCAATGCTGCTTCATCTCCTCCCGGTGCTGTTAATTACACTGCGCCATGTTGTGAAGTTGAAATGAATAGTACGACTGGGCTATGCACCCCGTTCAAGACCCCATGCCAGAACAGAACTCAGTATGCATTTTGGGATTCTATCCATCCTACTGAGATTTCAAATGTGGTTATAGCAGCAAGAGCATATAATGCTACGCTACCTACTGATGCTTTTCCATATGATATTAGTCACTTAGCTCAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

395

Amino Acids

43.21

Weight (kDa)

6.87

Isoelectric Point (pI)

29.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 67 - 373 2.3e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 806
AccII CGCG 1 cut(s) 389
AciI CCGC 3 cut(s) 84, 389, 649
AclWI GGATC 2 cut(s) 6, 79
AcoI YGGCCR 2 cut(s) 81, 258
AcsI RAATTY 1 cut(s) 64
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 4 cut(s) 43, 125, 719, 1012
AgsI TTSAA 4 cut(s) 850, 1001, 1036, 1101
AjnI CCWGG 1 cut(s) 471
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 104, 443, 532, 1178
AluI AGCT 4 cut(s) 104, 443, 532, 1178
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 2 cut(s) 6, 79
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 81, 258, 741
ApeKI GCWGC 6 cut(s) 392, 440, 554, 836, 947, 1115
ApoI RAATTY 1 cut(s) 64
AspLEI GCGC 2 cut(s) 599, 985
AspS9I GGNCC 2 cut(s) 302, 742
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 1 cut(s) 503
AsuII TTCGAA 1 cut(s) 780
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 806
BbvI GCAGC 6 cut(s) 379, 452, 541, 823, 934, 1127
BccI CCATC 3 cut(s) 170, 733, 1092
BceAI ACGGC 1 cut(s) 820
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 1 cut(s) 473
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 3 cut(s) 359, 645, 755
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 1 cut(s) 600
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 9 cut(s) 84, 390, 393, 441, 555, 649, 837, 948, 1116
BlsI GCNGC 9 cut(s) 85, 391, 394, 442, 556, 650, 838, 949, 1117
Bme1390I CCNGG 2 cut(s) 473, 963
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 302, 742
BmiI GGNNCC 2 cut(s) 769, 808
BmrFI CCNGG 2 cut(s) 473, 963
BmrI ACTGGG 1 cut(s) 1026
BmsI GCATC 3 cut(s) 191, 440, 1138
BmuI ACTGGG 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 780
BpuMI CCSGG 1 cut(s) 963
BsaAI YACGTR 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 472
Bse1I ACTGG 3 cut(s) 261, 313, 1021
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 1 cut(s) 1084
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 3 cut(s) 713, 1073, 1083
BseNI ACTGG 3 cut(s) 261, 313, 1021
BseRI GAGGAG 2 cut(s) 417, 948
BseXI GCAGC 6 cut(s) 379, 452, 541, 823, 934, 1127
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 3 cut(s) 83, 260, 743
BshNI GGYRCC 1 cut(s) 806
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 1 cut(s) 963
BslFI GGGAC 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 3 cut(s) 83, 260, 743
BsoBI CYCGRG 1 cut(s) 724
Bsp119I TTCGAA 1 cut(s) 780
Bsp1407I TGTACA 1 cut(s) 717
Bsp143I GATC 3 cut(s) 11, 71, 877
BspACI CCGC 3 cut(s) 84, 389, 649
BspANI GGCC 3 cut(s) 83, 260, 743
BspCNI CTCAG 3 cut(s) 712, 1072, 1084
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 2 cut(s) 769, 808
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 2 cut(s) 6, 79
BspT104I TTCGAA 1 cut(s) 780
BspT107I GGYRCC 1 cut(s) 806
BsrDI GCAATG 1 cut(s) 795
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 3 cut(s) 261, 313, 1021
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 3 cut(s) 11, 71, 877
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 2 cut(s) 8, 811
BstAPI GCANNNNNTGC 2 cut(s) 437, 1130
BstAUI TGTACA 1 cut(s) 717
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 780
BstDEI CTNAG 6 cut(s) 566, 699, 903, 1059, 1092, 1174
BstF5I GGATG 1 cut(s) 1084
BstFNI CGCG 1 cut(s) 389
BstH2I RGCGCY 1 cut(s) 600
BstHHI GCGC 2 cut(s) 599, 985
BstKTI GATC 3 cut(s) 14, 74, 880
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 3 cut(s) 11, 71, 877
BstMWI GCNNNNNNNGC 5 cut(s) 110, 437, 804, 1121, 1130
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 471, 961
BstSFI CTRYAG 1 cut(s) 555
BstSNI TACGTA 1 cut(s) 29
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 6 cut(s) 379, 452, 541, 823, 934, 1127
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 83, 260, 743
BtsCI GGATG 1 cut(s) 1084
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 3 cut(s) 254, 873, 978
CfoI GCGC 2 cut(s) 599, 985
Cfr13I GGNCC 2 cut(s) 302, 742
Csp6I GTAC 4 cut(s) 42, 124, 718, 1011
CviAII CATG 3 cut(s) 625, 987, 1044
CviQI GTAC 4 cut(s) 42, 124, 718, 1011
DdeI CTNAG 6 cut(s) 566, 699, 903, 1059, 1092, 1174
DpnI GATC 3 cut(s) 13, 73, 879
DpnII GATC 3 cut(s) 11, 71, 877
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 81, 258
Eco105I TACGTA 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 471
EcoT22I ATGCAT 3 cut(s) 165, 433, 1069
FaeI CATG 3 cut(s) 628, 990, 1047
FaqI GGGAC 1 cut(s) 261
FatI CATG 3 cut(s) 624, 986, 1043
FauNDI CATATG 1 cut(s) 1159
Fnu4HI GCNGC 9 cut(s) 84, 390, 393, 441, 555, 649, 837, 948, 1116
FokI GGATG 1 cut(s) 1071
Fsp4HI GCNGC 9 cut(s) 84, 390, 393, 441, 555, 649, 837, 948, 1116
FspBI CTAG 3 cut(s) 359, 645, 755
GlaI GCGC 2 cut(s) 598, 984
GluI GCNGC 9 cut(s) 84, 390, 393, 441, 555, 649, 837, 948, 1116
HaeII RGCGCY 1 cut(s) 600
HaeIII GGCC 3 cut(s) 83, 260, 743
HapII CCGG 1 cut(s) 963
HhaI GCGC 2 cut(s) 599, 985
Hin1II CATG 3 cut(s) 628, 990, 1047
Hin6I GCGC 2 cut(s) 597, 983
HinP1I GCGC 2 cut(s) 597, 983
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 4 cut(s) 215, 458, 712, 1076
HpaII CCGG 1 cut(s) 963
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 2 cut(s) 124, 548
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 3 cut(s) 95, 462, 1036
Hpy8I GTNNAC 2 cut(s) 124, 548
HpyAV CCTTC 2 cut(s) 306, 787
HpyCH4III ACNGT 2 cut(s) 8, 811
HpyCH4IV ACGT 3 cut(s) 28, 328, 466
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 437, 804, 1121, 1130
HpyF3I CTNAG 6 cut(s) 566, 699, 903, 1059, 1092, 1174
HpySE526I ACGT 3 cut(s) 28, 328, 466
Hsp92II CATG 3 cut(s) 628, 990, 1047
HspAI GCGC 2 cut(s) 597, 983
Kzo9I GATC 3 cut(s) 11, 71, 877
Lsp1109I GCAGC 6 cut(s) 379, 452, 541, 823, 934, 1127
LweI GCATC 3 cut(s) 191, 440, 1138
MaeI CTAG 3 cut(s) 359, 645, 755
MaeII ACGT 3 cut(s) 28, 328, 466
MaeIII GTNAC 6 cut(s) 197, 329, 452, 467, 871, 1169
MalI GATC 3 cut(s) 13, 73, 879
MboI GATC 3 cut(s) 11, 71, 877
MboII GAAGA 1 cut(s) 748
MfeI CAATTG 2 cut(s) 396, 500
MflI RGATCY 1 cut(s) 11
MluCI AATT 9 cut(s) 36, 64, 268, 396, 424, 500, 704, 930, 973
MlyI GAGTC 2 cut(s) 209, 706
MmeI TCCRAC 2 cut(s) 329, 345
MnlI CCTC 2 cut(s) 395, 969
Mph1103I ATGCAT 3 cut(s) 165, 433, 1069
MseI TTAA 5 cut(s) 62, 612, 861, 882, 972
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 1 cut(s) 963
MspR9I CCNGG 2 cut(s) 473, 963
MunI CAATTG 2 cut(s) 396, 500
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 5 cut(s) 110, 437, 804, 1121, 1130
NciI CCSGG 1 cut(s) 963
NdeI CATATG 1 cut(s) 1159
NdeII GATC 3 cut(s) 11, 71, 877
NlaIII CATG 3 cut(s) 628, 990, 1047
NlaIV GGNNCC 2 cut(s) 769, 808
NmuCI GTSAC 3 cut(s) 452, 871, 1169
NsiI ATGCAT 3 cut(s) 165, 433, 1069
NspV TTCGAA 1 cut(s) 780
PfeI GAWTC 2 cut(s) 458, 1076
PkrI GCNGC 9 cut(s) 85, 391, 394, 442, 556, 650, 838, 949, 1117
PleI GAGTC 2 cut(s) 209, 706
PpsI GAGTC 2 cut(s) 209, 706
Ppu21I YACGTR 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 2 cut(s) 769, 808
PspPI GGNCC 2 cut(s) 302, 742
PsrI GAACNNNNNNTAC 2 cut(s) 751, 783
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 11
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 4 cut(s) 43, 125, 719, 1012
RsaNI GTAC 4 cut(s) 42, 124, 718, 1011
SaqAI TTAA 5 cut(s) 62, 612, 861, 882, 972
SatI GCNGC 9 cut(s) 84, 390, 393, 441, 555, 649, 837, 948, 1116
Sau3AI GATC 3 cut(s) 11, 71, 877
Sau96I GGNCC 2 cut(s) 302, 742
SchI GAGTC 2 cut(s) 209, 706
ScrFI CCNGG 2 cut(s) 473, 963
SfaNI GCATC 3 cut(s) 191, 440, 1138
SfcI CTRYAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 780
SinI GGWCC 1 cut(s) 302
SnaBI TACGTA 1 cut(s) 29
Sse9I AATT 9 cut(s) 36, 64, 268, 396, 424, 500, 704, 930, 973
SsiI CCGC 3 cut(s) 84, 389, 649
SspMI CTAG 3 cut(s) 359, 645, 755
StyD4I CCNGG 2 cut(s) 471, 961
TaaI ACNGT 2 cut(s) 8, 811
TaiI ACGT 3 cut(s) 31, 331, 469
TaqI TCGA 4 cut(s) 155, 339, 584, 780
TasI AATT 9 cut(s) 36, 64, 268, 396, 424, 500, 704, 930, 973
TatI WGTACW 3 cut(s) 41, 123, 717
TauI GCSGC 3 cut(s) 86, 392, 651
TfiI GAWTC 2 cut(s) 458, 1076
Tru1I TTAA 5 cut(s) 62, 612, 861, 882, 972
Tru9I TTAA 5 cut(s) 62, 612, 861, 882, 972
TscAI CASTG 3 cut(s) 261, 880, 985
TseFI GTSAC 3 cut(s) 452, 871, 1169
TseI GCWGC 6 cut(s) 392, 440, 554, 836, 947, 1115
Tsp45I GTSAC 3 cut(s) 452, 871, 1169
TspDTI ATGAA 8 cut(s) 17, 141, 193, 384, 613, 898, 942, 1019
TspGWI ACGGA 3 cut(s) 339, 440, 724
TspRI CASTG 3 cut(s) 261, 880, 985
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 64
XspI CTAG 3 cut(s) 359, 645, 755
Zsp2I ATGCAT 3 cut(s) 165, 433, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.