Rw4G008350

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014711
Physical Location & Seq
Reverse (-)
18250503 .. 18253316
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008350.1

Sequence Viewer

Length: 1059 bp
ATGGGATCGGGTCGGAGGCGATGGCGTTTTGGAGCAGTGGTGGATGGACGGTTTCCTTGTTACTTCATATTTGGAGACTCGTTATCCGATAGTGGAAATAACAATGGGCTTTTGACACTGGCCAAAGTCAATTATCGTCCCTATGGCATTGACTTTCCACAAGGTCCAACTGGAAGGTTCTCCAACGGACGTAACTTGGTCGATATTATTGCTGAACTACTAGGTTTTGATGAATATATACCCACTTTCGCCGCTGCAATTGGAGGAGATATAACAAAAGGTGTAAATTATGCATCTGGTGCAGCTGGTATCCGTGACGAATCTGGACGTAACCAGGGAATGAGAATAACCTTGTATAAGCAATTGAGAAATCACCAAATCATAGTCAATAAGCTTGTTGCCAATGGTTCACTCGCTGCAGATTATCTAAGCAAGTGCATATATTCGATTGGGATAGGCACTAACGATTACATTAACAACTACTTCATGCCAAAACTCTACGCAACTAGCCGCTTATATACTCCAAAGCAATATGCAGATGTTCTTGTCGAAGAATACTCTCAGCAATTACGGACTCTGTACAACTCGGGGGCAAGGAAGATGGCCCTTATTGGACTAGGTACGATAGGTTCCACACCCTTCGAGGTGGCAATGTGTGGCATAAACGGCACCGTATGTGTTGATAACATCAACTATGCTGCCCAACTTTTCAATGCAAGGCTTAAATCACTTATCACTGATCTTAACACAAATCTAACAAATGCTAAGTTCATTTACATAGATTATTATGGAATTGGACAATCCAGTGCTGCTTCATCTCCTCCTGGTTCTGTTAATTACACTGCAGTGACTGCACCATGCTGTGAAGTTGAAATGAATAGTACGACGGGGCTATGCACCCCGTTCAAGACCCCATGCCAGAATAGAACTCAATATGCATTTTGGGATGCTATCCATCCTACTGAGATTTCAAATGTGGCTATAGCAGCAAGAGCATATAATGCTACGCTACCTACTGATGCTTTTCCATATGATATTAGTCACTTAGCTCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

352

Amino Acids

38.47

Weight (kDa)

8.32

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 330 9.2e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 668
AciI CCGC 2 cut(s) 252, 511
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 120
AfaI GTAC 3 cut(s) 581, 622, 883
AgsI TTSAA 4 cut(s) 712, 872, 907, 972
AjnI CCWGG 2 cut(s) 333, 823
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 3 cut(s) 305, 394, 1049
AluI AGCT 3 cut(s) 305, 394, 1049
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 851
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 2 cut(s) 120, 603
ApeKI GCWGC 6 cut(s) 254, 302, 416, 698, 809, 986
AspS9I GGNCC 2 cut(s) 164, 604
AsuHPI GGTGA 1 cut(s) 365
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 122
BanI GGYRCC 1 cut(s) 668
BbvI GCAGC 6 cut(s) 241, 314, 403, 685, 796, 998
BccI CCATC 4 cut(s) 15, 38, 595, 963
BceAI ACGGC 1 cut(s) 682
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 2 cut(s) 335, 825
BciVI GTATCC 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 3 cut(s) 221, 507, 617
BfmI CTRYAG 3 cut(s) 417, 843, 981
BfuI GTATCC 1 cut(s) 320
BisI GCNGC 8 cut(s) 252, 255, 303, 417, 511, 699, 810, 987
BlsI GCNGC 8 cut(s) 253, 256, 304, 418, 512, 700, 811, 988
Bme1390I CCNGG 2 cut(s) 335, 825
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 164, 604
BmiI GGNNCC 2 cut(s) 631, 670
BmrFI CCNGG 2 cut(s) 335, 825
BmsI GCATC 3 cut(s) 302, 937, 1009
BsaJI CCNNGG 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bse1I ACTGG 3 cut(s) 123, 175, 804
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 2 cut(s) 335, 825
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 3 cut(s) 49, 952, 955
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 575, 954
BseNI ACTGG 3 cut(s) 123, 175, 804
BseRI GAGGAG 2 cut(s) 279, 810
BseXI GCAGC 6 cut(s) 241, 314, 403, 685, 796, 998
BsgI GTGCAG 2 cut(s) 321, 837
BshFI GGCC 2 cut(s) 122, 605
BshNI GGYRCC 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 586
BslFI GGGAC 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 2 cut(s) 122, 605
BsoBI CYCGRG 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 2 cut(s) 5, 739
BspACI CCGC 2 cut(s) 252, 511
BspANI GGCC 2 cut(s) 122, 605
BspCNI CTCAG 2 cut(s) 574, 955
BspLI GGNNCC 2 cut(s) 631, 670
BspMAI CTGCAG 2 cut(s) 421, 847
BspPI GGATC 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 657
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 3 cut(s) 123, 175, 804
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 2 cut(s) 5, 739
Bst2UI CCWGG 2 cut(s) 335, 825
Bst4CI ACNGT 2 cut(s) 51, 673
BstAPI GCANNNNNTGC 3 cut(s) 299, 851, 1001
BstAUI TGTACA 1 cut(s) 579
BstDEI CTNAG 5 cut(s) 428, 561, 765, 963, 1045
BstF5I GGATG 3 cut(s) 49, 952, 955
BstKTI GATC 2 cut(s) 8, 742
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 5, 739
BstMWI GCNNNNNNNGC 6 cut(s) 299, 666, 851, 986, 992, 1001
BstNI CCWGG 2 cut(s) 335, 825
BstSCI CCNGG 2 cut(s) 333, 823
BstSFI CTRYAG 3 cut(s) 417, 843, 981
BstV1I GCAGC 6 cut(s) 241, 314, 403, 685, 796, 998
BsuI GTATCC 1 cut(s) 320
BsuRI GGCC 2 cut(s) 122, 605
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 3 cut(s) 49, 952, 955
BtsI GCAGTG 3 cut(s) 42, 840, 852
BtsIMutI CAGTG 6 cut(s) 42, 116, 735, 811, 840, 852
CaiI CAGNNNCTG 1 cut(s) 851
Cfr13I GGNCC 2 cut(s) 164, 604
Csp6I GTAC 3 cut(s) 580, 621, 882
CviAII CATG 3 cut(s) 487, 858, 915
CviQI GTAC 3 cut(s) 580, 621, 882
DdeI CTNAG 5 cut(s) 428, 561, 765, 963, 1045
DpnI GATC 2 cut(s) 7, 741
DpnII GATC 2 cut(s) 5, 739
EaeI YGGCCR 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 586
EcoRII CCWGG 2 cut(s) 333, 823
EcoT22I ATGCAT 2 cut(s) 295, 940
FaeI CATG 3 cut(s) 490, 861, 918
FaqI GGGAC 1 cut(s) 123
FatI CATG 3 cut(s) 486, 857, 914
FauNDI CATATG 1 cut(s) 1030
Fnu4HI GCNGC 8 cut(s) 252, 255, 303, 417, 511, 699, 810, 987
FokI GGATG 3 cut(s) 56, 942, 959
Fsp4HI GCNGC 8 cut(s) 252, 255, 303, 417, 511, 699, 810, 987
FspBI CTAG 3 cut(s) 221, 507, 617
GluI GCNGC 8 cut(s) 252, 255, 303, 417, 511, 699, 810, 987
HaeIII GGCC 2 cut(s) 122, 605
Hin1II CATG 3 cut(s) 490, 861, 918
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 3 cut(s) 77, 320, 574
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 1 cut(s) 410
Hpy188I TCNGA 2 cut(s) 15, 88
Hpy188III TCNNGA 2 cut(s) 324, 907
Hpy8I GTNNAC 1 cut(s) 410
Hpy99I CGWCG 1 cut(s) 889
HpyAV CCTTC 2 cut(s) 168, 649
HpyCH4III ACNGT 2 cut(s) 51, 673
HpyCH4IV ACGT 2 cut(s) 190, 328
HpyF10VI GCNNNNNNNGC 6 cut(s) 299, 666, 851, 986, 992, 1001
HpyF3I CTNAG 5 cut(s) 428, 561, 765, 963, 1045
HpySE526I ACGT 2 cut(s) 190, 328
Hsp92II CATG 3 cut(s) 490, 861, 918
Kzo9I GATC 2 cut(s) 5, 739
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 6 cut(s) 241, 314, 403, 685, 796, 998
LweI GCATC 3 cut(s) 302, 937, 1009
MaeI CTAG 3 cut(s) 221, 507, 617
MaeII ACGT 2 cut(s) 190, 328
MaeIII GTNAC 6 cut(s) 59, 191, 314, 329, 847, 1040
MalI GATC 2 cut(s) 7, 741
MboI GATC 2 cut(s) 5, 739
MboII GAAGA 2 cut(s) 563, 610
MfeI CAATTG 2 cut(s) 258, 362
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 7 cut(s) 130, 258, 286, 362, 566, 792, 835
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 2 cut(s) 71, 568
MmeI TCCRAC 2 cut(s) 191, 207
MnlI CCTC 4 cut(s) 9, 257, 637, 831
Mox20I TGGCCA 1 cut(s) 122
Mph1103I ATGCAT 2 cut(s) 295, 940
MscI TGGCCA 1 cut(s) 122
MseI TTAA 4 cut(s) 474, 723, 744, 834
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 122
MspA1I CMGCKG 2 cut(s) 254, 305
MspR9I CCNGG 2 cut(s) 335, 825
MunI CAATTG 2 cut(s) 258, 362
MvaI CCWGG 2 cut(s) 335, 825
MwoI GCNNNNNNNGC 6 cut(s) 299, 666, 851, 986, 992, 1001
NdeI CATATG 1 cut(s) 1030
NdeII GATC 2 cut(s) 5, 739
NlaIII CATG 3 cut(s) 490, 861, 918
NlaIV GGNNCC 2 cut(s) 631, 670
NmuCI GTSAC 3 cut(s) 314, 847, 1040
NsiI ATGCAT 2 cut(s) 295, 940
OliI CACNNNNGTG 1 cut(s) 845
PfeI GAWTC 1 cut(s) 320
PkrI GCNGC 8 cut(s) 253, 256, 304, 418, 512, 700, 811, 988
PleI GAGTC 2 cut(s) 71, 568
PpsI GAGTC 2 cut(s) 71, 568
Psp6I CCWGG 2 cut(s) 333, 823
PspGI CCWGG 2 cut(s) 333, 823
PspN4I GGNNCC 2 cut(s) 631, 670
PspPI GGNCC 2 cut(s) 164, 604
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstI CTGCAG 2 cut(s) 421, 847
PstNI CAGNNNCTG 1 cut(s) 851
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 3 cut(s) 581, 622, 883
RsaNI GTAC 3 cut(s) 580, 621, 882
RseI CAYNNNNRTG 1 cut(s) 845
SaqAI TTAA 4 cut(s) 474, 723, 744, 834
SatI GCNGC 8 cut(s) 252, 255, 303, 417, 511, 699, 810, 987
Sau3AI GATC 2 cut(s) 5, 739
Sau96I GGNCC 2 cut(s) 164, 604
SchI GAGTC 2 cut(s) 71, 568
ScrFI CCNGG 2 cut(s) 335, 825
SfaNI GCATC 3 cut(s) 302, 937, 1009
SfcI CTRYAG 3 cut(s) 417, 843, 981
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 845
Sse9I AATT 7 cut(s) 130, 258, 286, 362, 566, 792, 835
SsiI CCGC 2 cut(s) 252, 511
SspMI CTAG 3 cut(s) 221, 507, 617
StyD4I CCNGG 2 cut(s) 333, 823
TaaI ACNGT 2 cut(s) 51, 673
TaiI ACGT 2 cut(s) 193, 331
TaqI TCGA 4 cut(s) 201, 446, 549, 642
TasI AATT 7 cut(s) 130, 258, 286, 362, 566, 792, 835
TatI WGTACW 1 cut(s) 579
TauI GCSGC 2 cut(s) 254, 513
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 4 cut(s) 474, 723, 744, 834
Tru9I TTAA 4 cut(s) 474, 723, 744, 834
TscAI CASTG 6 cut(s) 42, 123, 742, 811, 847, 852
TseFI GTSAC 3 cut(s) 314, 847, 1040
TseI GCWGC 6 cut(s) 254, 302, 416, 698, 809, 986
Tsp45I GTSAC 3 cut(s) 314, 847, 1040
TspDTI ATGAA 6 cut(s) 55, 246, 475, 760, 804, 890
TspGWI ACGGA 3 cut(s) 201, 302, 586
TspRI CASTG 6 cut(s) 42, 123, 742, 811, 847, 852
VpaK11BI GGWCC 1 cut(s) 164
XspI CTAG 3 cut(s) 221, 507, 617
Zsp2I ATGCAT 2 cut(s) 295, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.