Rh4CG109800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014711
Physical Location & Seq
Reverse (-)
20606926 .. 20614662
7737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG109800.1

Sequence Viewer

Length: 822 bp
ATGCATTACTTGGTTGATGGGGCATCACAAGTTCCTTGTTACTTCATATTTGGAGACTCATTATCCGATAGTGGAAATAACAATGGGCTTTTGACACTGGCCAAAGTCAATTATCGTCCCTATGGCATTGACTTTCCACAAGGTCCAACTGGAAGGTTCTCCAACGGACGTAACTTGGCCGATATTATTGCTGAACTACTAGGTTTTGATGAATATACACCCACTTTCGCGGCTGCAATTGGAGGAGATATAACAAAAGGTGTAAATTATGCATCTGGTGCAGCTGGTATCCGTGACGAATCTGGACGTAACCAGGGAATGAGAATAACCTTGTATAAGCAATTGATAAATCACCAAATCATAGTCAATAAGCTTGTTGCCAATGGTTCACTCGCTGCAGATTATCTAAGCAAGTGCATATATTCGATTGGGATAGGCACTAACGATTACATTAACAACTACTTCATGCCAAAACTCTACCCAACTAGCCGCTTATATACTCCAAAGCAATATGCAGATGTTCTTGTCCAAGAATACTCTCAGCAATTACGGACTCTGTACAACTCGGGGGCAAGGAAGATGGCCCTTATTGGACTAGGTACGATAGGTTCCACACCCTTCGAAGTGGCAATGTGTGGCACAAACGGCACCGTATGTGTTGATAACATCAACAATGCTGCCCAACTTTTCAATGCAAGGCTTAAATCACTTGTCACTGATCTTAACACAAATCTCACAAATGCTAAGTTCATTTACATAGATTATTATGGAATTGGACTATCCAGTGTTGCTTCATCTCCTCCCGGTAATTTGATAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

273

Amino Acids

29.62

Weight (kDa)

8.22

Isoelectric Point (pI)

22.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 14 - 257 3.4e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccII CGCG 1 cut(s) 230
AciI CCGC 2 cut(s) 230, 490
AcoI YGGCCR 2 cut(s) 99, 177
AfaI GTAC 2 cut(s) 560, 601
AgsI TTSAA 1 cut(s) 691
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 2 cut(s) 284, 373
AluI AGCT 2 cut(s) 284, 373
Alw26I GTCTC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 3 cut(s) 99, 177, 582
ApeKI GCWGC 4 cut(s) 233, 281, 395, 677
AspS9I GGNCC 2 cut(s) 143, 583
AsuC2I CCSGG 1 cut(s) 804
AsuHPI GGTGA 1 cut(s) 344
AsuII TTCGAA 1 cut(s) 621
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 101
BanI GGYRCC 1 cut(s) 647
BbvI GCAGC 4 cut(s) 220, 293, 382, 664
BccI CCATC 2 cut(s) 11, 574
BceAI ACGGC 1 cut(s) 661
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 314
BciVI GTATCC 1 cut(s) 299
BcnI CCSGG 1 cut(s) 804
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 3 cut(s) 200, 486, 596
BfmI CTRYAG 1 cut(s) 396
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 6 cut(s) 231, 234, 282, 396, 490, 678
BlsI GCNGC 6 cut(s) 232, 235, 283, 397, 491, 679
Bme1390I CCNGG 2 cut(s) 314, 804
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 143, 583
BmiI GGNNCC 2 cut(s) 610, 649
BmrFI CCNGG 2 cut(s) 314, 804
BmsI GCATC 2 cut(s) 32, 281
Bpu14I TTCGAA 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 804
BsaJI CCNNGG 1 cut(s) 313
Bse1I ACTGG 3 cut(s) 102, 154, 783
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 313
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 3 cut(s) 102, 154, 783
BseRI GAGGAG 2 cut(s) 258, 789
BseXI GCAGC 4 cut(s) 220, 293, 382, 664
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 3 cut(s) 101, 179, 584
BshNI GGYRCC 1 cut(s) 647
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 1 cut(s) 804
BslFI GGGAC 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 3 cut(s) 101, 179, 584
BsoBI CYCGRG 1 cut(s) 565
Bsp119I TTCGAA 1 cut(s) 621
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 1 cut(s) 718
BspACI CCGC 2 cut(s) 230, 490
BspANI GGCC 3 cut(s) 101, 179, 584
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 2 cut(s) 610, 649
BspMAI CTGCAG 1 cut(s) 400
BspT104I TTCGAA 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 636
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 3 cut(s) 102, 154, 783
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 1 cut(s) 718
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 652
BstAPI GCANNNNNTGC 1 cut(s) 278
BstAUI TGTACA 1 cut(s) 558
BstBI TTCGAA 1 cut(s) 621
BstDEI CTNAG 3 cut(s) 407, 540, 744
BstFNI CGCG 1 cut(s) 230
BstKTI GATC 1 cut(s) 721
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 718
BstMWI GCNNNNNNNGC 2 cut(s) 278, 645
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 312, 802
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 220, 293, 382, 664
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 3 cut(s) 101, 179, 584
BtsIMutI CAGTG 3 cut(s) 95, 714, 790
Cfr13I GGNCC 2 cut(s) 143, 583
Csp6I GTAC 2 cut(s) 559, 600
CviAII CATG 1 cut(s) 466
CviJI RGCY 9 cut(s) 88, 101, 179, 233, 284, 373, 489, 584, 700
CviKI_1 RGCY 9 cut(s) 88, 101, 179, 233, 284, 373, 489, 584, 700
CviQI GTAC 2 cut(s) 559, 600
DdeI CTNAG 3 cut(s) 407, 540, 744
DpnI GATC 1 cut(s) 720
DpnII GATC 1 cut(s) 718
EaeI YGGCCR 2 cut(s) 99, 177
Eco47I GGWCC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 312
EcoT22I ATGCAT 2 cut(s) 6, 274
FaeI CATG 1 cut(s) 469
FaqI GGGAC 1 cut(s) 102
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 6 cut(s) 231, 234, 282, 396, 490, 678
Fsp4HI GCNGC 6 cut(s) 231, 234, 282, 396, 490, 678
FspBI CTAG 3 cut(s) 200, 486, 596
GluI GCNGC 6 cut(s) 231, 234, 282, 396, 490, 678
HaeIII GGCC 3 cut(s) 101, 179, 584
HapII CCGG 1 cut(s) 804
Hin1II CATG 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 56, 299, 553
HpaII CCGG 1 cut(s) 804
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 147, 628
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 2 cut(s) 169, 307
HpyCH4V TGCA 8 cut(s) 4, 236, 272, 281, 398, 417, 515, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 645
HpyF3I CTNAG 3 cut(s) 407, 540, 744
HpySE526I ACGT 2 cut(s) 169, 307
Hsp92II CATG 1 cut(s) 469
Kzo9I GATC 1 cut(s) 718
LpnPI CCDG 9 cut(s) 83, 135, 261, 270, 288, 299, 326, 796, 817
Lsp1109I GCAGC 4 cut(s) 220, 293, 382, 664
LweI GCATC 2 cut(s) 32, 281
MaeI CTAG 3 cut(s) 200, 486, 596
MaeII ACGT 2 cut(s) 169, 307
MaeIII GTNAC 5 cut(s) 38, 170, 293, 308, 712
MalI GATC 1 cut(s) 720
MboI GATC 1 cut(s) 718
MboII GAAGA 1 cut(s) 589
MfeI CAATTG 2 cut(s) 237, 341
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 7 cut(s) 109, 237, 265, 341, 545, 771, 808
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 2 cut(s) 50, 547
MmeI TCCRAC 2 cut(s) 170, 186
MnlI CCTC 2 cut(s) 236, 810
Mox20I TGGCCA 1 cut(s) 101
Mph1103I ATGCAT 2 cut(s) 6, 274
MscI TGGCCA 1 cut(s) 101
MseI TTAA 3 cut(s) 453, 702, 723
Msp20I TGGCCA 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 804
MspR9I CCNGG 2 cut(s) 314, 804
MunI CAATTG 2 cut(s) 237, 341
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 278, 645
NciI CCSGG 1 cut(s) 804
NdeII GATC 1 cut(s) 718
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 2 cut(s) 610, 649
NmuCI GTSAC 2 cut(s) 293, 712
NsiI ATGCAT 2 cut(s) 6, 274
NspV TTCGAA 1 cut(s) 621
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 6 cut(s) 232, 235, 283, 397, 491, 679
PleI GAGTC 2 cut(s) 50, 547
PpsI GAGTC 2 cut(s) 50, 547
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 610, 649
PspPI GGNCC 2 cut(s) 143, 583
PsrI GAACNNNNNNTAC 2 cut(s) 592, 624
PstI CTGCAG 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 560, 601
RsaNI GTAC 2 cut(s) 559, 600
SaqAI TTAA 3 cut(s) 453, 702, 723
SatI GCNGC 6 cut(s) 231, 234, 282, 396, 490, 678
Sau3AI GATC 1 cut(s) 718
Sau96I GGNCC 2 cut(s) 143, 583
SchI GAGTC 2 cut(s) 50, 547
ScrFI CCNGG 2 cut(s) 314, 804
SfaNI GCATC 2 cut(s) 32, 281
SfcI CTRYAG 1 cut(s) 396
SfuI TTCGAA 1 cut(s) 621
SinI GGWCC 1 cut(s) 143
Sse9I AATT 7 cut(s) 109, 237, 265, 341, 545, 771, 808
SsiI CCGC 2 cut(s) 230, 490
SspMI CTAG 3 cut(s) 200, 486, 596
StyD4I CCNGG 2 cut(s) 312, 802
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 2 cut(s) 172, 310
TaqI TCGA 2 cut(s) 425, 621
TasI AATT 7 cut(s) 109, 237, 265, 341, 545, 771, 808
TatI WGTACW 1 cut(s) 558
TauI GCSGC 2 cut(s) 233, 492
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 3 cut(s) 453, 702, 723
Tru9I TTAA 3 cut(s) 453, 702, 723
TscAI CASTG 3 cut(s) 102, 721, 790
TseFI GTSAC 2 cut(s) 293, 712
TseI GCWGC 4 cut(s) 233, 281, 395, 677
Tsp45I GTSAC 2 cut(s) 293, 712
TspDTI ATGAA 5 cut(s) 34, 225, 454, 739, 783
TspGWI ACGGA 3 cut(s) 180, 281, 565
TspRI CASTG 3 cut(s) 102, 721, 790
VpaK11BI GGWCC 1 cut(s) 143
XspI CTAG 3 cut(s) 200, 486, 596
Zsp2I ATGCAT 2 cut(s) 6, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.