RchiOBHm_Chr4g0400721

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014711
Physical Location & Seq
Reverse (-)
18467261 .. 18469128
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37271

Sequence Viewer

Length: 1185 bp
ATGTGCACTATATATAACACATTCTTCATCATTATTTGTACTTTTACTTTGTTGTTGTTAAATTTGGGATCATCAATGGCCGCCACACTTCGTGAAGTAGCTATAAAGGCATTGTGGGTGTACTTGTCTGTTTTGTTGTTGTTCGTTTCATCGAATATGCATTACTTGGTTGATGGGGCATCTCAAGTTCCTTGTTACTTCATATTTGGAGACTCGTTATCCGATAGTGGAAATAACAATGGGCTTTTGACACTGGCCAAAGTCAATTATCGTCCCTATGGCATTGACTTTCCACAAGGTCCAACTGGAAGGTTCTCCAACGGACGTAACTTGGTCGATATTATTGCTGAACTACTAGGTTTTGATGAATATATACCCACTTTCGCGGCTGCAATTGGAGGAGATATAACAAAAGGTGTAAATTATGCATCTGGTGCAGCTGGTATCCGTGACGAATCTGGACGTAACCAGGGAATGAGAATAACCTTGTATAAGCAATTGATAAATCACCAAATCATAGTCAATAAGCTTGTTGCCAATGGTTCACTCGCTGCAGATTATCTAAGCAAGTGCATATATTCGATTGCGATAGGCGCTAACGATTACATTAACAACTACTTCATGCCAAAACTCTACCCAACTAGCCGCTTATATACTCCAAAGCAATATGCAGATGTTCTTGTCGAAGAATGCTCTCAGCAATTACGGACTCTGTACAACTCGGGGGCAAGGAAGATGGCCCTTATTGGACTAGGCACGATAGGTTCCACACCCTTCGAAGTGGCAATGTGTGGCATAAACGGCACCGTATGTGTTGATAACATCAACTATGCTGCCCAACTTTTCAATGCAAGGCTTAAATCACTTGTCACTGATCTTAACACAAATCTAACAAATGCTATGTTCATTTACATAGATTATTATGGAATTGGACTATCCAGTGCTGCTTCATCTCCTCCTGGTTCTGTTAATTACACTGCACCATGCTGTGAAGTTGAAATGAATAGTACGACGGGGCTATGCACCCCGTTCAAGACCCCATGCCAGAATAGAACTCAATATGCATTTTGGGATGCTATCCATCCTACTGAGATTTCAAATGTGGTTATAGCAGCAAGAGCATATAATGCTACGCTACCTACTGATGCTTTTCCATATGATATTAGTCACTTAGCTCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

394

Amino Acids

43.03

Weight (kDa)

5.82

Isoelectric Point (pI)

29.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 66 - 372 1.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014711)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07670
malus_domestica MD12G1049900.v1.1
prunus_persica Prupe.1G081300_v2.0.a1 Prupe.1G081300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0400521 RchiOBHm_Chr4g0400721
rosa_laevigata RLG00000009183
rosa_multiflora Rmu_sc0002636.1_g000017
rosa_roxburghii Rroxscaffold_5G00345560
rosa_rugosa Rorug04G0025400
rosa_samantha Rh4AG101800 Rh4AG103200 Rh4BG098100 Rh4CG109800 Rh4CG111500 Rh4DG095100
rosa_wichuraiana Rw4G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 803
AccII CGCG 1 cut(s) 386
AciI CCGC 3 cut(s) 81, 386, 646
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 2 cut(s) 78, 255
AcsI RAATTY 1 cut(s) 61
AdeI CACNNNGTG 1 cut(s) 92
AfaI GTAC 4 cut(s) 40, 122, 716, 1009
AgsI TTSAA 4 cut(s) 847, 998, 1033, 1098
AjnI CCWGG 2 cut(s) 468, 958
AluBI AGCT 4 cut(s) 101, 440, 529, 1175
AluI AGCT 4 cut(s) 101, 440, 529, 1175
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 204
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 721
AoxI GGCC 3 cut(s) 78, 255, 738
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 6 cut(s) 389, 437, 551, 833, 944, 1112
ApoI RAATTY 1 cut(s) 61
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 2 cut(s) 299, 739
AsuHPI GGTGA 1 cut(s) 500
AsuII TTCGAA 1 cut(s) 777
AvaI CYCGRG 1 cut(s) 721
AvaII GGWCC 1 cut(s) 299
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 803
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 6 cut(s) 376, 449, 538, 820, 931, 1124
BccI CCATC 3 cut(s) 167, 730, 1089
BceAI ACGGC 1 cut(s) 817
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 2 cut(s) 470, 960
BciVI GTATCC 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 3 cut(s) 356, 642, 752
BfmI CTRYAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 597
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 9 cut(s) 81, 387, 390, 438, 552, 646, 834, 945, 1113
BlsI GCNGC 9 cut(s) 82, 388, 391, 439, 553, 647, 835, 946, 1114
Bme1390I CCNGG 2 cut(s) 470, 960
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 721
BmgT120I GGNCC 2 cut(s) 299, 739
BmiI GGNNCC 2 cut(s) 766, 805
BmrFI CCNGG 2 cut(s) 470, 960
BmsI GCATC 4 cut(s) 188, 437, 1063, 1135
Bpu14I TTCGAA 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 469
Bse1I ACTGG 3 cut(s) 258, 310, 939
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 2 cut(s) 470, 960
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 2 cut(s) 1078, 1081
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 2 cut(s) 710, 1080
BseNI ACTGG 3 cut(s) 258, 310, 939
BseRI GAGGAG 2 cut(s) 414, 945
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 6 cut(s) 376, 449, 538, 820, 931, 1124
BsgI GTGCAG 2 cut(s) 456, 963
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 3 cut(s) 80, 257, 740
BshNI GGYRCC 1 cut(s) 803
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 721
BslFI GGGAC 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 3 cut(s) 80, 257, 740
BsoBI CYCGRG 1 cut(s) 721
Bsp119I TTCGAA 1 cut(s) 777
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 68, 874
BspACI CCGC 3 cut(s) 81, 386, 646
BspANI GGCC 3 cut(s) 80, 257, 740
BspCNI CTCAG 2 cut(s) 709, 1081
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 2 cut(s) 766, 805
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 1 cut(s) 76
BspT104I TTCGAA 1 cut(s) 777
BspT107I GGYRCC 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 792
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 258, 310, 939
BssECI CCNNGG 1 cut(s) 469
BssMI GATC 2 cut(s) 68, 874
Bst2UI CCWGG 2 cut(s) 470, 960
Bst4CI ACNGT 1 cut(s) 808
BstAPI GCANNNNNTGC 2 cut(s) 434, 1127
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 777
BstDEI CTNAG 4 cut(s) 563, 696, 1089, 1171
BstF5I GGATG 2 cut(s) 1078, 1081
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 2 cut(s) 71, 877
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 68, 874
BstMWI GCNNNNNNNGC 6 cut(s) 107, 434, 593, 801, 1118, 1127
BstNI CCWGG 2 cut(s) 470, 960
BstSCI CCNGG 2 cut(s) 468, 958
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 6 cut(s) 376, 449, 538, 820, 931, 1124
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 3 cut(s) 80, 257, 740
BtsCI GGATG 2 cut(s) 1078, 1081
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 4 cut(s) 251, 870, 946, 975
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 2 cut(s) 299, 739
Csp6I GTAC 4 cut(s) 39, 121, 715, 1008
CviAII CATG 3 cut(s) 622, 984, 1041
CviQI GTAC 4 cut(s) 39, 121, 715, 1008
DdeI CTNAG 4 cut(s) 563, 696, 1089, 1171
DpnI GATC 2 cut(s) 70, 876
DpnII GATC 2 cut(s) 68, 874
DraIII CACNNNGTG 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 78, 255
Eco47I GGWCC 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 721
EcoRII CCWGG 2 cut(s) 468, 958
EcoT22I ATGCAT 3 cut(s) 162, 430, 1066
FaeI CATG 3 cut(s) 625, 987, 1044
FaqI GGGAC 1 cut(s) 258
FatI CATG 3 cut(s) 621, 983, 1040
FauNDI CATATG 1 cut(s) 1156
Fnu4HI GCNGC 9 cut(s) 81, 387, 390, 438, 552, 646, 834, 945, 1113
FokI GGATG 2 cut(s) 1068, 1085
Fsp4HI GCNGC 9 cut(s) 81, 387, 390, 438, 552, 646, 834, 945, 1113
FspBI CTAG 3 cut(s) 356, 642, 752
GlaI GCGC 1 cut(s) 595
GluI GCNGC 9 cut(s) 81, 387, 390, 438, 552, 646, 834, 945, 1113
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 3 cut(s) 80, 257, 740
HhaI GCGC 1 cut(s) 596
Hin1II CATG 3 cut(s) 625, 987, 1044
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 3 cut(s) 212, 455, 709
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 3 cut(s) 6, 121, 545
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 92, 459, 1033
Hpy8I GTNNAC 3 cut(s) 6, 121, 545
Hpy99I CGWCG 1 cut(s) 1015
HpyAV CCTTC 2 cut(s) 303, 784
HpyCH4III ACNGT 1 cut(s) 808
HpyCH4IV ACGT 2 cut(s) 325, 463
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 434, 593, 801, 1118, 1127
HpyF3I CTNAG 4 cut(s) 563, 696, 1089, 1171
HpySE526I ACGT 2 cut(s) 325, 463
Hsp92II CATG 3 cut(s) 625, 987, 1044
HspAI GCGC 1 cut(s) 594
Kzo9I GATC 2 cut(s) 68, 874
Lsp1109I GCAGC 6 cut(s) 376, 449, 538, 820, 931, 1124
LweI GCATC 4 cut(s) 188, 437, 1063, 1135
MaeI CTAG 3 cut(s) 356, 642, 752
MaeII ACGT 2 cut(s) 325, 463
MaeIII GTNAC 6 cut(s) 194, 326, 449, 464, 868, 1166
MalI GATC 2 cut(s) 70, 876
MboI GATC 2 cut(s) 68, 874
MboII GAAGA 3 cut(s) 16, 698, 745
MfeI CAATTG 2 cut(s) 393, 497
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 8 cut(s) 61, 265, 393, 421, 497, 701, 927, 970
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 2 cut(s) 206, 703
MmeI TCCRAC 2 cut(s) 326, 342
MnlI CCTC 2 cut(s) 392, 966
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 3 cut(s) 162, 430, 1066
MscI TGGCCA 1 cut(s) 257
MseI TTAA 5 cut(s) 59, 609, 858, 879, 969
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 440
MspR9I CCNGG 2 cut(s) 470, 960
MunI CAATTG 2 cut(s) 393, 497
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 2 cut(s) 470, 960
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 6 cut(s) 107, 434, 593, 801, 1118, 1127
NdeI CATATG 1 cut(s) 1156
NdeII GATC 2 cut(s) 68, 874
NlaIII CATG 3 cut(s) 625, 987, 1044
NlaIV GGNNCC 2 cut(s) 766, 805
NmuCI GTSAC 3 cut(s) 449, 868, 1166
NsiI ATGCAT 3 cut(s) 162, 430, 1066
NspV TTCGAA 1 cut(s) 777
PctI GAATGC 1 cut(s) 695
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 9 cut(s) 82, 388, 391, 439, 553, 647, 835, 946, 1114
PleI GAGTC 2 cut(s) 206, 703
PpsI GAGTC 2 cut(s) 206, 703
Psp6I CCWGG 2 cut(s) 468, 958
PspGI CCWGG 2 cut(s) 468, 958
PspN4I GGNNCC 2 cut(s) 766, 805
PspPI GGNCC 2 cut(s) 299, 739
PstI CTGCAG 1 cut(s) 556
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 4 cut(s) 40, 122, 716, 1009
RsaNI GTAC 4 cut(s) 39, 121, 715, 1008
SaqAI TTAA 5 cut(s) 59, 609, 858, 879, 969
SatI GCNGC 9 cut(s) 81, 387, 390, 438, 552, 646, 834, 945, 1113
Sau3AI GATC 2 cut(s) 68, 874
Sau96I GGNCC 2 cut(s) 299, 739
SchI GAGTC 2 cut(s) 206, 703
ScrFI CCNGG 2 cut(s) 470, 960
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 4 cut(s) 188, 437, 1063, 1135
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 777
SinI GGWCC 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
Sse9I AATT 8 cut(s) 61, 265, 393, 421, 497, 701, 927, 970
SsiI CCGC 3 cut(s) 81, 386, 646
SspMI CTAG 3 cut(s) 356, 642, 752
StyD4I CCNGG 2 cut(s) 468, 958
TaaI ACNGT 1 cut(s) 808
TaiI ACGT 2 cut(s) 328, 466
TaqI TCGA 5 cut(s) 152, 336, 581, 684, 777
TasI AATT 8 cut(s) 61, 265, 393, 421, 497, 701, 927, 970
TatI WGTACW 3 cut(s) 38, 120, 714
TauI GCSGC 3 cut(s) 83, 389, 648
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 5 cut(s) 59, 609, 858, 879, 969
Tru9I TTAA 5 cut(s) 59, 609, 858, 879, 969
TscAI CASTG 4 cut(s) 258, 877, 946, 982
TseFI GTSAC 3 cut(s) 449, 868, 1166
TseI GCWGC 6 cut(s) 389, 437, 551, 833, 944, 1112
Tsp45I GTSAC 3 cut(s) 449, 868, 1166
TspDTI ATGAA 8 cut(s) 16, 138, 190, 381, 610, 895, 939, 1016
TspGWI ACGGA 3 cut(s) 336, 437, 721
TspRI CASTG 4 cut(s) 258, 877, 946, 982
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 61
XspI CTAG 3 cut(s) 356, 642, 752
Zsp2I ATGCAT 3 cut(s) 162, 430, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.