Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014159

Physical Location & Seq

Genomic Coordinates Forward (+)

6180217 .. 6180798

Gene Structure 582 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1054500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 582 bp

ATGGCCACCGCCTCCTTCCTCTCCGGCCTCAAGTACTCCGACAGCTTGACGGTGGTGGGCATATCCGTCTGCACCGCCATCATCTGCGAGGCGATCTCGTGGGTCCTCATCTACCGCACCAACTCCTACAAGTCCCTCCGCTCCTCCATCGACAAAGCCGCCAAGAAGCTCGAGACCATGAAGACCGACTCCTCCTCCGCCGCCAAAATTACCAAAAAATCCAAGACCAAGAAGATCGACCGCGTCGAGACCAGCCTCAAGGAATCCAGCCGGGATTTGTCGCTCTTCAAGTTCAAGTCCGGCGCTGTCGTCGCCCTCGTCCTCTTCGTCGTCTTCGGGCTGCTCAACTCGCTGTTTGAGGGTAAGCCGGTGGCGAAATTACCCTTCAAGCCCTTTGGGCTGGTGATGAAGATGAGCCACCGAGGGCTGCAAGGGGAGGATCCGACGGACTGTTCGATGGCGTTTTTGTACTTTCTGTGCTCGATCAGTATTCGGACCAATTTGCAGAAATTCTTGGGCTTTTCGCCACCGAGAGGTGCCAGTTCCGGGTTGTTCCCCATGCCCGACCCGAAAACCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006983 GO:0006984 GO:0007154 GO:0007165 GO:0008150 GO:0008324 GO:0009607 GO:0009987 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0019725 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0030003 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0032469 GO:0033554 GO:0034220 GO:0034976 GO:0042175 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071216 GO:0072503 GO:0072507 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF106

Protein Analysis

194

Amino Acids

21.1

Weight (kDa)

9.79

Isoelectric Point (pI)

29.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMC3_TMCO1	PF01956	16 - 175	2.1e-32	Integral membrane protein EMC3/TMCO1-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014159)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12030 AT5G06660
fragaria_vesca	FvH4_6g21840
malus_domestica	MD00G1019800.v1.1 MD12G1054500.v1.1 MD14G1053500.v1.1
prunus_persica	Prupe.7G082200_v2.0.a1
pyrus_communis	pycom11g10000 pycom12g04750
rosa_chinensis	RchiOBHm_Chr3g0479191
rosa_laevigata	RLG00000023597
rosa_multiflora	Rmu_sc0002978.1_g000022 Rmu_sc0015470.1_g000009
rosa_roxburghii	Rroxscaffold_6G00402570
rosa_rugosa	Rorug03G0172000
rosa_samantha	Rh3CG252000
rosa_wichuraiana	Rw3G020030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	536
AccBSI	CCGCTC	1 cut(s)	141
AccII	CGCG	1 cut(s)	243
AciI	CCGC	8 cut(s)	9, 75, 115, 139, 159, 198, 201, 241
AclWI	GGATC	2 cut(s)	434, 447
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	509
AfaI	GTAC	2 cut(s)	35, 470
AfiI	CCNNNNNNNGG	2 cut(s)	533, 546
AgsI	TTSAA	3 cut(s)	289, 295, 388
AluBI	AGCT	2 cut(s)	45, 169
AluI	AGCT	2 cut(s)	45, 169
Alw21I	GWGCWC	1 cut(s)	482
Alw26I	GTCTC	2 cut(s)	167, 242
AlwI	GGATC	2 cut(s)	434, 447
Ama87I	CYCGRG	1 cut(s)	170
AoxI	GGCC	2 cut(s)	3, 25
ApeKI	GCWGC	2 cut(s)	340, 427
ApoI	RAATTY	1 cut(s)	509
ArsI	GACNNNNNNTTYG	2 cut(s)	436, 468
AspLEI	GCGC	1 cut(s)	305
AspS9I	GGNCC	2 cut(s)	103, 495
AsuC2I	CCSGG	2 cut(s)	272, 547
AsuHPI	GGTGA	1 cut(s)	415
AvaI	CYCGRG	1 cut(s)	170
AvaII	GGWCC	2 cut(s)	103, 495
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	439
BanI	GGYRCC	1 cut(s)	536
BauI	CACGAG	1 cut(s)	97
BbsI	GAAGAC	2 cut(s)	188, 325
Bbv12I	GWGCWC	1 cut(s)	482
BbvI	GCAGC	2 cut(s)	327, 414
BccI	CCATC	3 cut(s)	86, 155, 451
BcnI	CCSGG	2 cut(s)	272, 547
BcoDI	GTCTC	2 cut(s)	167, 242
BfoI	RGCGCY	1 cut(s)	306
BglI	GCCNNNNNGGC	1 cut(s)	397
BisI	GCNGC	4 cut(s)	159, 201, 341, 428
BlsI	GCNGC	4 cut(s)	160, 202, 342, 429
BmcAI	AGTACT	1 cut(s)	35
Bme1390I	CCNGG	2 cut(s)	272, 547
Bme18I	GGWCC	2 cut(s)	103, 495
BmeT110I	CYCGRG	1 cut(s)	170
BmgT120I	GGNCC	2 cut(s)	103, 495
BmiI	GGNNCC	3 cut(s)	104, 441, 538
BmrFI	CCNGG	2 cut(s)	272, 547
BpiI	GAAGAC	2 cut(s)	188, 325
BplI	GAGNNNNNCTC	2 cut(s)	80, 112
BpuEI	CTTGAG	2 cut(s)	14, 242
BpuMI	CCSGG	2 cut(s)	272, 547
BsaI	GGTCTC	2 cut(s)	167, 242
BsaJI	CCNNGG	1 cut(s)	421
BsaXI	ACNNNNNCTCC	2 cut(s)	179, 209
Bsc4I	CCNNNNNNNGG	2 cut(s)	533, 546
Bse118I	RCCGGY	1 cut(s)	367
Bse1I	ACTGG	1 cut(s)	540
BseDI	CCNNGG	1 cut(s)	421
BseLI	CCNNNNNNNGG	2 cut(s)	533, 546
BseNI	ACTGG	1 cut(s)	540
BseRI	GAGGAG	3 cut(s)	133, 181, 184
BseXI	GCAGC	2 cut(s)	327, 414
BsgI	GTGCAG	1 cut(s)	55
Bsh1236I	CGCG	1 cut(s)	243
Bsh1285I	CGRYCG	1 cut(s)	241
BshFI	GGCC	2 cut(s)	5, 27
BshNI	GGYRCC	1 cut(s)	536
BsiEI	CGRYCG	1 cut(s)	241
BsiHKAI	GWGCWC	1 cut(s)	482
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	5 cut(s)	24, 271, 300, 368, 546
BslFI	GGGAC	1 cut(s)	118
BslI	CCNNNNNNNGG	2 cut(s)	533, 546
BsmAI	GTCTC	2 cut(s)	167, 242
BsmFI	GGGAC	1 cut(s)	118
BsnI	GGCC	2 cut(s)	5, 27
Bso31I	GGTCTC	2 cut(s)	167, 242
BsoBI	CYCGRG	1 cut(s)	170
Bsp1286I	GDGCHC	1 cut(s)	482
Bsp143I	GATC	4 cut(s)	93, 234, 439, 483
BspACI	CCGC	8 cut(s)	9, 75, 115, 139, 159, 198, 201, 241
BspANI	GGCC	2 cut(s)	5, 27
BspFNI	CGCG	1 cut(s)	243
BspLI	GGNNCC	3 cut(s)	104, 441, 538
BspPI	GGATC	2 cut(s)	434, 447
BspQI	GCTCTTC	1 cut(s)	290
BspT107I	GGYRCC	1 cut(s)	536
BspTNI	GGTCTC	2 cut(s)	167, 242
BsrBI	CCGCTC	1 cut(s)	141
BsrFI	RCCGGY	1 cut(s)	367
BsrI	ACTGG	1 cut(s)	540
BssAI	RCCGGY	1 cut(s)	367
BssECI	CCNNGG	1 cut(s)	421
BssMI	GATC	4 cut(s)	93, 234, 439, 483
BssSI	CACGAG	1 cut(s)	97
Bst2BI	CACGAG	1 cut(s)	97
Bst4CI	ACNGT	2 cut(s)	52, 452
Bst6I	CTCTTC	2 cut(s)	290, 329
BstFNI	CGCG	1 cut(s)	243
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	1 cut(s)	305
BstKTI	GATC	4 cut(s)	96, 237, 442, 486
BstMAI	GTCTC	2 cut(s)	167, 242
BstMBI	GATC	4 cut(s)	93, 234, 439, 483
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	3 cut(s)	311, 349, 397
BstSCI	CCNGG	2 cut(s)	270, 545
BstUI	CGCG	1 cut(s)	243
BstV1I	GCAGC	2 cut(s)	327, 414
BstV2I	GAAGAC	2 cut(s)	188, 325
BstX2I	RGATCY	1 cut(s)	439
BstYI	RGATCY	1 cut(s)	439
BsuRI	GGCC	2 cut(s)	5, 27
CfoI	GCGC	1 cut(s)	305
Cfr10I	RCCGGY	1 cut(s)	367
Cfr13I	GGNCC	2 cut(s)	103, 495
CseI	GACGC	1 cut(s)	232
Csp6I	GTAC	2 cut(s)	34, 469
CviAII	CATG	2 cut(s)	178, 559
CviQI	GTAC	2 cut(s)	34, 469
DpnI	GATC	4 cut(s)	95, 236, 441, 485
DpnII	GATC	4 cut(s)	93, 234, 439, 483
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	290, 329
EarI	CTCTTC	2 cut(s)	290, 329
EciI	GGCGGA	1 cut(s)	187
Eco31I	GGTCTC	2 cut(s)	167, 242
Eco47I	GGWCC	2 cut(s)	103, 495
Eco88I	CYCGRG	1 cut(s)	170
EcoO109I	RGGNCCY	1 cut(s)	103
FaeI	CATG	2 cut(s)	181, 562
FaiI	YATR	3 cut(s)	62, 179, 560
FaqI	GGGAC	1 cut(s)	118
FatI	CATG	2 cut(s)	177, 558
Fnu4HI	GCNGC	4 cut(s)	159, 201, 341, 428
Fsp4HI	GCNGC	4 cut(s)	159, 201, 341, 428
GlaI	GCGC	1 cut(s)	304
GluI	GCNGC	4 cut(s)	159, 201, 341, 428
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	2 cut(s)	5, 27
HapII	CCGG	5 cut(s)	24, 271, 300, 368, 546
HgaI	GACGC	1 cut(s)	232
HhaI	GCGC	1 cut(s)	305
Hin1II	CATG	2 cut(s)	181, 562
Hin6I	GCGC	1 cut(s)	303
HinP1I	GCGC	1 cut(s)	303
HinfI	GANTC	2 cut(s)	188, 263
HpaII	CCGG	5 cut(s)	24, 271, 300, 368, 546
HphI	GGTGA	1 cut(s)	415
Hpy188I	TCNGA	3 cut(s)	40, 444, 495
Hpy188III	TCNNGA	2 cut(s)	172, 247
Hpy99I	CGWCG	4 cut(s)	248, 314, 332, 448
HpyAV	CCTTC	2 cut(s)	25, 394
HpyCH4III	ACNGT	2 cut(s)	52, 452
HpyCH4V	TGCA	3 cut(s)	72, 430, 505
HpyF10VI	GCNNNNNNNGC	3 cut(s)	311, 349, 397
Hsp92II	CATG	2 cut(s)	181, 562
HspAI	GCGC	1 cut(s)	303
Kzo9I	GATC	4 cut(s)	93, 234, 439, 483
LguI	GCTCTTC	1 cut(s)	290
LmnI	GCTCC	1 cut(s)	146
LpnPI	CCDG	9 cut(s)	37, 265, 280, 284, 313, 381, 386, 553, 559
Lsp1109I	GCAGC	2 cut(s)	327, 414
MalI	GATC	4 cut(s)	95, 236, 441, 485
MbiI	CCGCTC	1 cut(s)	141
MboI	GATC	4 cut(s)	93, 234, 439, 483
MboII	GAAGA	6 cut(s)	193, 244, 277, 316, 325, 421
MfeI	CAATTG	1 cut(s)	577
MflI	RGATCY	1 cut(s)	439
MhlI	GDGCHC	1 cut(s)	482
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	207, 377, 499, 509, 577
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	182
MmeI	TCCRAC	2 cut(s)	63, 467
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	5 cut(s)	24, 271, 300, 368, 546
MspR9I	CCNGG	2 cut(s)	272, 547
MunI	CAATTG	1 cut(s)	577
MvnI	CGCG	1 cut(s)	243
MwoI	GCNNNNNNNGC	3 cut(s)	311, 349, 397
NciI	CCSGG	2 cut(s)	272, 547
NdeII	GATC	4 cut(s)	93, 234, 439, 483
NlaIII	CATG	2 cut(s)	181, 562
NlaIV	GGNNCC	3 cut(s)	104, 441, 538
PaeR7I	CTCGAG	1 cut(s)	170
PciSI	GCTCTTC	1 cut(s)	290
PcsI	WCGNNNNNNNCGW	4 cut(s)	243, 315, 324, 452
PfeI	GAWTC	1 cut(s)	263
PflFI	GACNNNGTC	1 cut(s)	242
PkrI	GCNGC	4 cut(s)	160, 202, 342, 429
PleI	GAGTC	1 cut(s)	182
PpsI	GAGTC	1 cut(s)	182
PpuMI	RGGWCCY	1 cut(s)	103
Psp5II	RGGWCCY	1 cut(s)	103
PspN4I	GGNNCC	3 cut(s)	104, 441, 538
PspPI	GGNCC	2 cut(s)	103, 495
PspPPI	RGGWCCY	1 cut(s)	103
PsuI	RGATCY	1 cut(s)	439
PsyI	GACNNNGTC	1 cut(s)	242
RsaI	GTAC	2 cut(s)	35, 470
RsaNI	GTAC	2 cut(s)	34, 469
SapI	GCTCTTC	1 cut(s)	290
SatI	GCNGC	4 cut(s)	159, 201, 341, 428
Sau3AI	GATC	4 cut(s)	93, 234, 439, 483
Sau96I	GGNCC	2 cut(s)	103, 495
ScaI	AGTACT	1 cut(s)	35
SchI	GAGTC	1 cut(s)	182
ScrFI	CCNGG	2 cut(s)	272, 547
SduI	GDGCHC	1 cut(s)	482
SetI	ASST	3 cut(s)	47, 171, 538
Sfr274I	CTCGAG	1 cut(s)	170
SinI	GGWCC	2 cut(s)	103, 495
SlaI	CTCGAG	1 cut(s)	170
SmlI	CTYRAG	3 cut(s)	29, 170, 257
SmoI	CTYRAG	3 cut(s)	29, 170, 257
Sse9I	AATT	5 cut(s)	207, 377, 499, 509, 577
SsiI	CCGC	8 cut(s)	9, 75, 115, 139, 159, 198, 201, 241
StyD4I	CCNGG	2 cut(s)	270, 545
TaaI	ACNGT	2 cut(s)	52, 452
TaqI	TCGA	6 cut(s)	150, 171, 237, 246, 455, 482
TaqII	GACCGA	1 cut(s)	200
TasI	AATT	5 cut(s)	207, 377, 499, 509, 577
TatI	WGTACW	2 cut(s)	33, 468
TauI	GCSGC	2 cut(s)	161, 203
TfiI	GAWTC	1 cut(s)	263
TseI	GCWGC	2 cut(s)	340, 427
TspDTI	ATGAA	2 cut(s)	194, 422
TspGWI	ACGGA	2 cut(s)	55, 461
Tth111I	GACNNNGTC	1 cut(s)	242
VpaK11BI	GGWCC	2 cut(s)	103, 495
XapI	RAATTY	1 cut(s)	509
XhoI	CTCGAG	1 cut(s)	170
ZrmI	AGTACT	1 cut(s)	35

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.