MD12G1057500.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014158
Physical Location & Seq
Reverse (-)
6564506 .. 6565024
519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1057500.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGAGGAGGCTCAGACACTAACCAAGAAGCAAACTTTCTGCTTAAATATGCCAGAAGCACTTGCAATCCACAGACACTCACAAACAATATCAAAACCAAAGCCCAAAATCCGAATTATTCACATATATGCCCCAGAGATCATCAAGACTGACGTGGCAAACTTCAGGGAGCTGGTTCAGAGACTCACCGGAAAACCATCGGAAAACGGCGGCTGCAGCAACAGGAGGAACAACAAGAAAAACCCAACAAGTATACCCAGCAGAGAAGGACCCAAACCCAGCAAAAAGAATGCATTGATCATGGCTGACAAGATGAGAGCTGGGTGCAAGGGATTTGTGGACCAATCGAGAGACAGAATTAAGGGAGAAGAAGGGCTGATGTGGAATGCAGAAAACTCTGGTGGGTTTCTTGAGAGATTTGCAGATTTGGAGGGGTTTATTCAGGAATTTGGTGGTGAATTTCCTTTGATACCCACTATGGACGCTCCAAATTCTCACCTCCTGCATGGCTTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

173

Amino Acids

19.27

Weight (kDa)

9.35

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 42 - 67 2.1e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42140 AT3G58000
fragaria_vesca FvH4_6g22390
malus_domestica MD12G1057500.v1.1 MD14G1058100.v1.1
prunus_persica Prupe.7G076600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0479721
rosa_laevigata RLG00000023546
rosa_multiflora Rmu_sc0003755.1_g000010 Rmu_sc0031395.1_g000004
rosa_roxburghii Rroxscaffold_6G00402040
rosa_rugosa Rorug03G0177900
rosa_samantha Rh3BG261600 Rh3CG258700 Rh3DG254900
rosa_wichuraiana Rw0G015320 Rw3G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 253
AciI CCGC 1 cut(s) 210
AcsI RAATTY 3 cut(s) 446, 458, 490
AcuI CTGAAG 1 cut(s) 148
AjiI CACGTC 1 cut(s) 154
AluBI AGCT 2 cut(s) 172, 320
AluI AGCT 2 cut(s) 172, 320
Alw26I GTCTC 2 cut(s) 175, 345
ApeKI GCWGC 2 cut(s) 213, 216
ApoI RAATTY 3 cut(s) 446, 458, 490
AspS9I GGNCC 2 cut(s) 269, 340
AsuHPI GGTGA 3 cut(s) 178, 467, 488
AvaII GGWCC 2 cut(s) 269, 340
BbvI GCAGC 2 cut(s) 200, 228
BccI CCATC 1 cut(s) 205
BceAI ACGGC 1 cut(s) 223
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 175, 345
BfmI CTRYAG 1 cut(s) 214
BisI GCNGC 3 cut(s) 211, 214, 217
BlsI GCNGC 3 cut(s) 212, 215, 218
Bme18I GGWCC 2 cut(s) 269, 340
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 269, 340
BmiI GGNNCC 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 431
BsaWI WCCGGW 1 cut(s) 188
BseMII CTCAG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 200, 228
BseYI CCCAGC 3 cut(s) 257, 278, 320
BsiSI CCGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 175, 345
BsmI GAATGC 2 cut(s) 295, 391
Bsp143I GATC 2 cut(s) 138, 297
BspACI CCGC 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 218
BssMI GATC 2 cut(s) 138, 297
BssNAI GTATAC 1 cut(s) 254
Bst1107I GTATAC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 12
BstKTI GATC 2 cut(s) 141, 300
BstMAI GTCTC 2 cut(s) 175, 345
BstMBI GATC 2 cut(s) 138, 297
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstSFI CTRYAG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 200, 228
BstZ17I GTATAC 1 cut(s) 254
BtrI CACGTC 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 269, 340
CseI GACGC 1 cut(s) 491
CviAII CATG 2 cut(s) 301, 506
CviJI RGCY 8 cut(s) 11, 103, 172, 213, 305, 320, 376, 510
CviKI_1 RGCY 8 cut(s) 11, 103, 172, 213, 305, 320, 376, 510
DdeI CTNAG 1 cut(s) 12
DpnI GATC 2 cut(s) 140, 299
DpnII GATC 2 cut(s) 138, 297
Eco47I GGWCC 2 cut(s) 269, 340
Eco57I CTGAAG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 269
EcoT22I ATGCAT 1 cut(s) 295
FaeI CATG 2 cut(s) 304, 509
FaiI YATR 8 cut(s) 50, 125, 127, 129, 254, 302, 479, 507
FatI CATG 2 cut(s) 300, 505
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 253
Fnu4HI GCNGC 3 cut(s) 211, 214, 217
Fsp4HI GCNGC 3 cut(s) 211, 214, 217
GluI GCNGC 3 cut(s) 211, 214, 217
GsaI CCCAGC 3 cut(s) 261, 282, 324
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 491
Hin1II CATG 2 cut(s) 304, 509
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 189
HphI GGTGA 3 cut(s) 178, 467, 488
Hpy166II GTNNAC 2 cut(s) 254, 340
Hpy188I TCNGA 4 cut(s) 15, 113, 180, 202
Hpy188III TCNNGA 4 cut(s) 145, 348, 410, 443
Hpy8I GTNNAC 2 cut(s) 254, 340
HpyAV CCTTC 2 cut(s) 260, 365
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 7 cut(s) 65, 216, 293, 327, 389, 422, 505
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 2 cut(s) 304, 509
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 2 cut(s) 138, 297
LmnI GCTCC 2 cut(s) 169, 490
Lsp1109I GCAGC 2 cut(s) 200, 228
MaeII ACGT 1 cut(s) 153
MalI GATC 2 cut(s) 140, 299
MboI GATC 2 cut(s) 138, 297
MboII GAAGA 1 cut(s) 380
MluCI AATT 6 cut(s) 114, 357, 446, 458, 490, 513
MlyI GAGTC 1 cut(s) 177
MnlI CCTC 3 cut(s) 219, 424, 509
Mph1103I ATGCAT 1 cut(s) 295
MseI TTAA 3 cut(s) 44, 360, 512
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 1 cut(s) 189
Mva1269I GAATGC 2 cut(s) 295, 391
MwoI GCNNNNNNNGC 1 cut(s) 216
NdeII GATC 2 cut(s) 138, 297
NlaIII CATG 2 cut(s) 304, 509
NlaIV GGNNCC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 295
PctI GAATGC 2 cut(s) 295, 391
PkrI GCNGC 3 cut(s) 212, 215, 218
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 269
PspFI CCCAGC 3 cut(s) 257, 278, 320
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 269, 340
PspPPI RGGWCCY 1 cut(s) 269
PstI CTGCAG 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 3 cut(s) 44, 360, 512
SatI GCNGC 3 cut(s) 211, 214, 217
Sau3AI GATC 2 cut(s) 138, 297
Sau96I GGNCC 2 cut(s) 269, 340
SchI GAGTC 1 cut(s) 177
SetI ASST 4 cut(s) 156, 174, 322, 501
SfcI CTRYAG 1 cut(s) 214
SinI GGWCC 2 cut(s) 269, 340
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 114, 357, 446, 458, 490, 513
SsiI CCGC 1 cut(s) 210
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 347
TasI AATT 6 cut(s) 114, 357, 446, 458, 490, 513
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 3 cut(s) 44, 360, 512
Tru9I TTAA 3 cut(s) 44, 360, 512
TseI GCWGC 2 cut(s) 213, 216
VpaK11BI GGWCC 2 cut(s) 269, 340
XapI RAATTY 3 cut(s) 446, 458, 490
XmiI GTMKAC 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.