Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007308

Physical Location & Seq

Genomic Coordinates Forward (+)

8095775 .. 8100815

Gene Structure 5041 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1068200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGCCTGAACTGGTCGAACATCTCCCTAAATCGTATCTCCTGCTGTGCATCTTTGTTGTGTTCCTGTTACGTACTCTTTCTGGTGCAGAGATTCCCTTAGGTTCAAAACTTTCTGTGGCCGATAAAAACATATGGATCTCTCCAAATGGTGATTTTGTGTTTGGATTTTTTAACTGTTCAGACCAGCCAAACTATAGTTTTGGGATCCGTTTCAATTCAAAATCCATTCCGCTTGACCAACAACTTTTAGTATGGAGTGCTGCAGATGATCTTATTCTTGGTAACGATTCTTATGCCCAGCTGACCCAGGATGGTGAACTAGTTTTATTTGATTCCTTGAAGGGTGTCACTGCATGGACCAGTAAAACAAGGCAATTATCAGTTGTTTCTGCTGCTCTTAATGACAATGGAAATCTTGTTCTATTGAATCAAGAGAAACATATTGTTTGGCAAAGTTTTGATTCTCCATCTGACATACTTCTTCCTGTACAGAACTTCTCTACATTTCAAACACTTCGAGCTGCAAGCAGGAATAGTGTGTCCAGTTACTACACTCTTTTCATGAGTGCTTCTAGTCAGTTGGAGCTTCGATGGGAAAGTCATGTCACCTACTGGACAAGTGGAAGCCCTCCTAGTTCAAACCTCAGTGCTTTCATCACTTCTGATGGAGCCCTACAACTCCGTGATCAAAACTTGAAACCTGTGTGGTCACTATATGGTCAAGATCACAATGACTCTGTAAGTTACAGGTTTCTTAGGCTAGATGTTGATGGTAATCTCCGGTTATACTCATGGGTAGAAGAATCAGAGTCATGGAGATCAGTCTGGCAAGCAGTTGAGAACCAGTGTGATGTCTTTGCGACCTGTGGCCAACGTGGCATCTGTGTTTTCAATGAATCTGGGTTCCCTGATTGCGAATGTCCATTTAGGCAGCAGACAAATGAGTCCACTTCCAGATGTTTTATACCAAATCATCGGTGTGCTTCTGGTTCCAACATGCATCATTATAAGCATACTTCTCTACATGGAATGTACCCACCAACTGATGATGTAGTTGCCAAAGTTAGTCTAGAGAAATGCAAAAGCTTGTGTCAGAATGACCTGACTTGTACAGCTACAACCTTTACAAATGATGGAACTGCACGATGCTTAATTAAGAGAACACCGTATGTAACTGGCTATTCAGACCCCTCACTGAGTTCAGTATCTTACGTGAAAATGTGTGCAGATCCATTAGCTGTAAATCCCAATCTCTCGTTGCCTTCCCCTCCTCTCAATCGGTCTCATAAGTTTTGTTTCCCTTGCTTAATCGGAGCAGCCTCAGGAGTGTTCGTCGTATTTGTTTTAGTTCAATTGGCACTTGGTTTCTGGTTCTACAGAAGAAGAAATTTGGATAGGAAGAAAGCCGCGTTTGCTCATACCAGCCCAAACTCGAATGGTTTGATAGTGTTATCCTTCTCTGAAATAGAGGAGCTTACAGAGAACTTCAAATATCAAATTGGGCCAAAGATGTTCAAAGGTGTTCTTCCAAATAGAAAACAAGTTGCAATCAAAGATGTGGATGCAAGCGTAGAAGAAAGAAAATACCGGTGTGTAGTTGCAAAGATAGGAAACATTCATCACAAAAGCCTTGTGAAGCTGCAGGGCTACTGTTGTGAGTTAGATCACAGATTTCTGGTCTATGAATATGTCAAGAATGGTTCTCTGGAAAAGTATATAGAAGATGTTAAACTGTGTAAGAAGCTGACTTGGGGGAAGAGATTTGATATATGCTTAAGTGTCGCAAGGGCCATTTGTTATCTGCACACAAGCTGTAGGGAATTTATGAGCCATGGGAATTTGAAATGCGAGAATGTGGTACTGGATGAAAATTTAGAGGCCAAGGTGACTGAATTTGGACTTGGGAACATAGTCAGCAAGGCATCATGCTCTTCATGCTCTTCTGCAGAGAGAGATGTGGAGGACTTTGGTAAGATGGTGTTAGTATTAGTAAGTGGGTGTCGAGTTGGGGACCTTTGTGAGTGGGCATACGAAGAGTGGCTGCAAGGGCATCCAGAGAATGTGGTAGATAGGAAAATAATTGGTGGGTTTATTGTGCAAGAGCTGGAACGTGCTTTAAGAATTGCATTTTGGTGTGTCCAAACTGATGAACGTAGAAGACCTCCAATGAGAGAGGTGGTTAAGGTCTTGGAGGGCACCTTGAGCGTTGATCCACCACCACCCCCTTTTGCTTGTCAAGGGCCACTTGATGACGAAGAGGAGCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

756

Amino Acids

84.97

Weight (kDa)

6.06

Isoelectric Point (pI)

43.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	84 - 168	1.9e-14	D-mannose binding lectin
S_locus_glycop	PF00954	244 - 307	6.5e-12	S-locus glycoprotein domain
PK_Tyr_Ser-Thr	PF07714	511 - 642	9.8e-18	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	511 - 642	1.5e-14	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007308)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41890
fragaria_vesca	FvH4_6g26380 FvH4_6g26380
malus_domestica	MD12G1068200.v1.1 MD14G1068300.v1.1 MD14G1068400.v1.1 MD14G1068600.v1.1 MD14G1068700.v1.1 MD14G1069000.v1.1
prunus_persica	Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1 Prupe.7G065700_v2.0.a1
pyrus_communis	pycom12g06020 pycom14g05640
rosa_chinensis	RchiOBHm_Chr2g0126871
rosa_laevigata	RLG00000018924
rosa_multiflora	Rmu_sc0000007.1_g000026
rosa_roxburghii	Rroxscaffold_2G00116950
rosa_rugosa	Rorug02G0270000
rosa_samantha	Rh2AG322100 Rh2BG330300 Rh2CG310200 Rh2DG349600
rosa_wichuraiana	Rw2G026120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
AasI	GACNNNNNNGTC	1 cut(s)	943
AccB1I	GGYRCC	1 cut(s)	2195
AccII	CGCG	1 cut(s)	1409
AciI	CCGC	2 cut(s)	230, 1407
AclWI	GGATC	5 cut(s)	143, 199, 212, 1223, 2204
AcoI	YGGCCR	2 cut(s)	117, 868
AcsI	RAATTY	5 cut(s)	1387, 1820, 1837, 1870, 1892
AfaI	GTAC	5 cut(s)	73, 489, 1034, 1111, 1860
AflII	CTTAAG	1 cut(s)	1774
AgeI	ACCGGT	1 cut(s)	1587
AhlI	ACTAGT	1 cut(s)	319
AjnI	CCWGG	1 cut(s)	306
AjuI	GAANNNNNNNTTGG	4 cut(s)	1884, 1916, 2113, 2145
AloI	GAACNNNNNNTCC	2 cut(s)	402, 434
Alw26I	GTCTC	1 cut(s)	1287
AlwI	GGATC	5 cut(s)	143, 199, 212, 1223, 2204
AoxI	GGCC	6 cut(s)	117, 868, 1502, 1788, 1878, 2240
ApeKI	GCWGC	7 cut(s)	260, 392, 521, 931, 1316, 1639, 2041
ApoI	RAATTY	5 cut(s)	1387, 1820, 1837, 1870, 1892
AsiGI	ACCGGT	1 cut(s)	1587
AspS9I	GGNCC	5 cut(s)	357, 1502, 1788, 2011, 2240
AsuHPI	GGTGA	4 cut(s)	161, 326, 598, 1897
AvaII	GGWCC	2 cut(s)	357, 2011
AxyI	CCTNAGG	2 cut(s)	97, 1321
BaeGI	GKGCMC	1 cut(s)	2198
BalI	TGGCCA	1 cut(s)	870
BamHI	GGATCC	1 cut(s)	204
BanI	GGYRCC	1 cut(s)	2195
BanII	GRGCYC	1 cut(s)	673
BbsI	GAAGAC	1 cut(s)	2164
BbvI	GCAGC	7 cut(s)	247, 379, 508, 943, 1328, 1626, 2028
BccI	CCATC	7 cut(s)	305, 475, 585, 659, 764, 1127, 1969
BceAI	ACGGC	1 cut(s)	2248
BcgI	CGANNNNNNTGC	2 cut(s)	275, 309
BciT130I	CCWGG	1 cut(s)	308
BclI	TGATCA	1 cut(s)	685
BcoDI	GTCTC	1 cut(s)	1287
BcuI	ACTAGT	1 cut(s)	319
BfaI	CTAG	5 cut(s)	320, 573, 633, 761, 1070
BfmI	CTRYAG	6 cut(s)	193, 261, 1375, 1640, 1813, 1944
BfrI	CTTAAG	1 cut(s)	1774
BglI	GCCNNNNNGGC	1 cut(s)	876
BisI	GCNGC	8 cut(s)	261, 393, 522, 932, 1317, 1407, 1640, 2042
BlsI	GCNGC	8 cut(s)	262, 394, 523, 933, 1318, 1408, 1641, 2043
Bme1390I	CCNGG	1 cut(s)	308
Bme18I	GGWCC	2 cut(s)	357, 2011
BmgT120I	GGNCC	5 cut(s)	357, 1502, 1788, 2011, 2240
BmiI	GGNNCC	7 cut(s)	206, 670, 905, 991, 2012, 2197, 2262
BmrFI	CCNGG	1 cut(s)	308
BmsI	GCATC	7 cut(s)	57, 888, 1009, 1136, 1552, 1931, 2059
BpiI	GAAGAC	1 cut(s)	2164
BpuEI	CTTGAG	1 cut(s)	2221
BsaAI	YACGTR	2 cut(s)	71, 1213
BsaBI	GATNNNNATC	1 cut(s)	774
BsaI	GGTCTC	1 cut(s)	1287
BsaJI	CCNNGG	3 cut(s)	306, 1831, 1881
BsaWI	WCCGGW	2 cut(s)	780, 1587
BsaXI	ACNNNNNCTCC	2 cut(s)	1317, 1347
Bse118I	RCCGGY	1 cut(s)	1587
Bse1I	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1866
Bse21I	CCTNAGG	2 cut(s)	97, 1321
Bse8I	GATNNNNATC	1 cut(s)	774
BseBI	CCWGG	1 cut(s)	308
BseDI	CCNNGG	3 cut(s)	306, 1831, 1881
BseGI	GGATG	4 cut(s)	316, 1567, 1870, 2050
BseJI	GATNNNNATC	1 cut(s)	774
BseMII	CTCAG	3 cut(s)	658, 1187, 1335
BseNI	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1866
BseRI	GAGGAG	2 cut(s)	1260, 1484
BseSI	GKGCMC	1 cut(s)	2198
BseXI	GCAGC	7 cut(s)	247, 379, 508, 943, 1328, 1626, 2028
BseYI	CCCAGC	1 cut(s)	297
BsgI	GTGCAG	4 cut(s)	105, 1125, 1245, 1787
Bsh1236I	CGCG	1 cut(s)	1409
BshFI	GGCC	6 cut(s)	119, 870, 1504, 1790, 1880, 2242
BshNI	GGYRCC	1 cut(s)	2195
BshTI	ACCGGT	1 cut(s)	1587
BsiSI	CCGG	2 cut(s)	781, 1588
BslFI	GGGAC	1 cut(s)	2024
BsmAI	GTCTC	1 cut(s)	1287
BsmFI	GGGAC	1 cut(s)	2024
BsnI	GGCC	6 cut(s)	119, 870, 1504, 1790, 1880, 2242
Bso31I	GGTCTC	1 cut(s)	1287
Bsp1286I	GDGCHC	2 cut(s)	673, 2198
Bsp1407I	TGTACA	2 cut(s)	487, 1109
Bsp143I	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
Bsp19I	CCATGG	1 cut(s)	1831
BspACI	CCGC	2 cut(s)	230, 1407
BspANI	GGCC	6 cut(s)	119, 870, 1504, 1790, 1880, 2242
BspCNI	CTCAG	3 cut(s)	657, 1188, 1334
BspFNI	CGCG	1 cut(s)	1409
BspHI	TCATGA	1 cut(s)	561
BspLI	GGNNCC	7 cut(s)	206, 670, 905, 991, 2012, 2197, 2262
BspMAI	CTGCAG	3 cut(s)	265, 1644, 1948
BspPI	GGATC	5 cut(s)	143, 199, 212, 1223, 2204
BspQI	GCTCTTC	2 cut(s)	1936, 1945
BspT107I	GGYRCC	1 cut(s)	2195
BspTI	CTTAAG	1 cut(s)	1774
BspTNI	GGTCTC	1 cut(s)	1287
BsrFI	RCCGGY	1 cut(s)	1587
BsrGI	TGTACA	2 cut(s)	487, 1109
BsrI	ACTGG	7 cut(s)	15, 360, 543, 617, 844, 1180, 1866
BssAI	RCCGGY	1 cut(s)	1587
BssECI	CCNNGG	3 cut(s)	306, 1831, 1881
BssMI	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
BssT1I	CCWWGG	2 cut(s)	1831, 1881
Bst2UI	CCWGG	1 cut(s)	308
Bst4CI	ACNGT	4 cut(s)	176, 1167, 1652, 1734
Bst6I	CTCTTC	5 cut(s)	1751, 1936, 1945, 2028, 2250
BstAFI	CTTAAG	1 cut(s)	1774
BstAUI	TGTACA	2 cut(s)	487, 1109
BstBAI	YACGTR	2 cut(s)	71, 1213
BstC8I	GCNNGC	3 cut(s)	526, 831, 1567
BstDEI	CTNAG	5 cut(s)	97, 644, 755, 1196, 1321
BstDSI	CCRYGG	1 cut(s)	1831
BstF5I	GGATG	4 cut(s)	316, 1567, 1870, 2050
BstFNI	CGCG	1 cut(s)	1409
BstKTI	GATC	9 cut(s)	138, 207, 271, 688, 727, 821, 1231, 1666, 2212
BstMAI	GTCTC	1 cut(s)	1287
BstMBI	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
BstMWI	GCNNNNNNNGC	5 cut(s)	876, 1412, 1935, 2047, 2202
BstNI	CCWGG	1 cut(s)	308
BstNSI	RCATGY	1 cut(s)	1000
BstSCI	CCNGG	1 cut(s)	306
BstSFI	CTRYAG	6 cut(s)	193, 261, 1375, 1640, 1813, 1944
BstSLI	GKGCMC	1 cut(s)	2198
BstSNI	TACGTA	1 cut(s)	71
BstUI	CGCG	1 cut(s)	1409
BstV1I	GCAGC	7 cut(s)	247, 379, 508, 943, 1328, 1626, 2028
BstV2I	GAAGAC	1 cut(s)	2164
BstX2I	RGATCY	3 cut(s)	135, 204, 1228
BstYI	RGATCY	3 cut(s)	135, 204, 1228
Bsu36I	CCTNAGG	2 cut(s)	97, 1321
BsuRI	GGCC	6 cut(s)	119, 870, 1504, 1790, 1880, 2242
BtgI	CCRYGG	1 cut(s)	1831
BtsCI	GGATG	4 cut(s)	316, 1567, 1870, 2050
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	4 cut(s)	348, 652, 851, 1193
Cac8I	GCNNGC	3 cut(s)	526, 831, 1567
CciI	TCATGA	1 cut(s)	561
Cfr10I	RCCGGY	1 cut(s)	1587
Cfr13I	GGNCC	5 cut(s)	357, 1502, 1788, 2011, 2240
Csp6I	GTAC	5 cut(s)	72, 488, 1033, 1110, 1859
CspAI	ACCGGT	1 cut(s)	1587
CviQI	GTAC	5 cut(s)	72, 488, 1033, 1110, 1859
DdeI	CTNAG	5 cut(s)	97, 644, 755, 1196, 1321
DpnI	GATC	9 cut(s)	137, 206, 270, 687, 726, 820, 1230, 1665, 2211
DpnII	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
DrdI	GACNNNNNNGTC	1 cut(s)	943
DseDI	GACNNNNNNGTC	1 cut(s)	943
EaeI	YGGCCR	2 cut(s)	117, 868
Eam1104I	CTCTTC	5 cut(s)	1751, 1936, 1945, 2028, 2250
EarI	CTCTTC	5 cut(s)	1751, 1936, 1945, 2028, 2250
Eco105I	TACGTA	1 cut(s)	71
Eco130I	CCWWGG	2 cut(s)	1831, 1881
Eco24I	GRGCYC	1 cut(s)	673
Eco31I	GGTCTC	1 cut(s)	1287
Eco47I	GGWCC	2 cut(s)	357, 2011
Eco81I	CCTNAGG	2 cut(s)	97, 1321
EcoO109I	RGGNCCY	1 cut(s)	2011
EcoRII	CCWGG	1 cut(s)	306
EcoT14I	CCWWGG	2 cut(s)	1831, 1881
EcoT22I	ATGCAT	1 cut(s)	1002
EcoT38I	GRGCYC	1 cut(s)	673
ErhI	CCWWGG	2 cut(s)	1831, 1881
FalI	AAGNNNNNCTT	4 cut(s)	1509, 1541, 2229, 2261
FaqI	GGGAC	1 cut(s)	2024
FauNDI	CATATG	1 cut(s)	131
FbaI	TGATCA	1 cut(s)	685
Fnu4HI	GCNGC	8 cut(s)	261, 393, 522, 932, 1317, 1407, 1640, 2042
FokI	GGATG	4 cut(s)	323, 1574, 1877, 2037
FriOI	GRGCYC	1 cut(s)	673
Fsp4HI	GCNGC	8 cut(s)	261, 393, 522, 932, 1317, 1407, 1640, 2042
FspBI	CTAG	5 cut(s)	320, 573, 633, 761, 1070
GluI	GCNGC	8 cut(s)	261, 393, 522, 932, 1317, 1407, 1640, 2042
GsaI	CCCAGC	1 cut(s)	301
HaeIII	GGCC	6 cut(s)	119, 870, 1504, 1790, 1880, 2242
HapII	CCGG	2 cut(s)	781, 1588
HindIII	AAGCTT	1 cut(s)	1084
HpaII	CCGG	2 cut(s)	781, 1588
HphI	GGTGA	4 cut(s)	161, 326, 598, 1897
Hpy166II	GTNNAC	2 cut(s)	317, 948
Hpy188I	TCNGA	8 cut(s)	181, 472, 664, 808, 1095, 1186, 1313, 1462
Hpy188III	TCNNGA	9 cut(s)	431, 562, 722, 954, 1070, 1323, 1693, 1706, 2054
Hpy8I	GTNNAC	2 cut(s)	317, 948
Hpy99I	CGWCG	1 cut(s)	1337
HpyAV	CCTTC	3 cut(s)	334, 1272, 1465
HpyCH4III	ACNGT	4 cut(s)	176, 1167, 1652, 1734
HpyCH4IV	ACGT	5 cut(s)	70, 874, 1212, 2110, 2152
HpyF10VI	GCNNNNNNNGC	5 cut(s)	876, 1412, 1935, 2047, 2202
HpyF3I	CTNAG	5 cut(s)	97, 644, 755, 1196, 1321
HpySE526I	ACGT	5 cut(s)	70, 874, 1212, 2110, 2152
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
LguI	GCTCTTC	2 cut(s)	1936, 1945
LmnI	GCTCC	5 cut(s)	583, 668, 1313, 1471, 2260
Lsp1109I	GCAGC	7 cut(s)	247, 379, 508, 943, 1328, 1626, 2028
LweI	GCATC	7 cut(s)	57, 888, 1009, 1136, 1552, 1931, 2059
MaeI	CTAG	5 cut(s)	320, 573, 633, 761, 1070
MaeII	ACGT	5 cut(s)	70, 874, 1212, 2110, 2152
MaeIII	GTNAC	9 cut(s)	66, 281, 346, 545, 604, 708, 743, 1171, 1885
MalI	GATC	9 cut(s)	137, 206, 270, 687, 726, 820, 1230, 1665, 2211
MboI	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
MfeI	CAATTG	1 cut(s)	1352
MflI	RGATCY	3 cut(s)	135, 204, 1228
MhlI	GDGCHC	2 cut(s)	673, 2198
MlsI	TGGCCA	1 cut(s)	870
MluNI	TGGCCA	1 cut(s)	870
MlyI	GAGTC	3 cut(s)	728, 818, 953
MmeI	TCCRAC	2 cut(s)	561, 1017
Mox20I	TGGCCA	1 cut(s)	870
Mph1103I	ATGCAT	1 cut(s)	1002
MscI	TGGCCA	1 cut(s)	870
MseI	TTAA	9 cut(s)	171, 399, 1151, 1155, 1307, 1728, 1775, 2117, 2181
MslI	CAYNNNNRTG	2 cut(s)	978, 2131
Msp20I	TGGCCA	1 cut(s)	870
MspA1I	CMGCKG	1 cut(s)	301
MspCI	CTTAAG	1 cut(s)	1774
MspI	CCGG	2 cut(s)	781, 1588
MspR9I	CCNGG	1 cut(s)	308
MunI	CAATTG	1 cut(s)	1352
MvaI	CCWGG	1 cut(s)	308
MvnI	CGCG	1 cut(s)	1409
MwoI	GCNNNNNNNGC	5 cut(s)	876, 1412, 1935, 2047, 2202
NcoI	CCATGG	1 cut(s)	1831
NdeI	CATATG	1 cut(s)	131
NdeII	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
NlaIV	GGNNCC	7 cut(s)	206, 670, 905, 991, 2012, 2197, 2262
NmuCI	GTSAC	4 cut(s)	346, 604, 708, 1885
NsiI	ATGCAT	1 cut(s)	1002
NspI	RCATGY	1 cut(s)	1000
PacI	TTAATTAA	1 cut(s)	1155
PagI	TCATGA	1 cut(s)	561
PciSI	GCTCTTC	2 cut(s)	1936, 1945
PfeI	GAWTC	7 cut(s)	91, 287, 332, 427, 461, 803, 896
PinAI	ACCGGT	1 cut(s)	1587
PkrI	GCNGC	8 cut(s)	262, 394, 523, 933, 1318, 1408, 1641, 2043
PleI	GAGTC	3 cut(s)	728, 817, 952
PpsI	GAGTC	3 cut(s)	728, 817, 952
Ppu21I	YACGTR	2 cut(s)	71, 1213
PpuMI	RGGWCCY	1 cut(s)	2011
PsiI	TTATAA	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	2011
Psp6I	CCWGG	1 cut(s)	306
PspFI	CCCAGC	1 cut(s)	297
PspGI	CCWGG	1 cut(s)	306
PspN4I	GGNNCC	7 cut(s)	206, 670, 905, 991, 2012, 2197, 2262
PspPI	GGNCC	5 cut(s)	357, 1502, 1788, 2011, 2240
PspPPI	RGGWCCY	1 cut(s)	2011
PstI	CTGCAG	3 cut(s)	265, 1644, 1948
PsuI	RGATCY	3 cut(s)	135, 204, 1228
PvuII	CAGCTG	1 cut(s)	301
RsaI	GTAC	5 cut(s)	73, 489, 1034, 1111, 1860
RsaNI	GTAC	5 cut(s)	72, 488, 1033, 1110, 1859
RseI	CAYNNNNRTG	2 cut(s)	978, 2131
SapI	GCTCTTC	2 cut(s)	1936, 1945
SaqAI	TTAA	9 cut(s)	171, 399, 1151, 1155, 1307, 1728, 1775, 2117, 2181
SatI	GCNGC	8 cut(s)	261, 393, 522, 932, 1317, 1407, 1640, 2042
Sau3AI	GATC	9 cut(s)	135, 204, 268, 685, 724, 818, 1228, 1663, 2209
Sau96I	GGNCC	5 cut(s)	357, 1502, 1788, 2011, 2240
SchI	GAGTC	3 cut(s)	728, 818, 953
ScrFI	CCNGG	1 cut(s)	308
SduI	GDGCHC	2 cut(s)	673, 2198
SfaNI	GCATC	7 cut(s)	57, 888, 1009, 1136, 1552, 1931, 2059
SfcI	CTRYAG	6 cut(s)	193, 261, 1375, 1640, 1813, 1944
SinI	GGWCC	2 cut(s)	357, 2011
SmiMI	CAYNNNNRTG	2 cut(s)	978, 2131
SmlI	CTYRAG	2 cut(s)	1774, 2200
SmoI	CTYRAG	2 cut(s)	1774, 2200
SnaBI	TACGTA	1 cut(s)	71
SpeI	ACTAGT	1 cut(s)	319
SsiI	CCGC	2 cut(s)	230, 1407
SspMI	CTAG	5 cut(s)	320, 573, 633, 761, 1070
StyD4I	CCNGG	1 cut(s)	306
StyI	CCWWGG	2 cut(s)	1831, 1881
TaaI	ACNGT	4 cut(s)	176, 1167, 1652, 1734
TaiI	ACGT	5 cut(s)	73, 877, 1215, 2113, 2155
TaqI	TCGA	5 cut(s)	15, 517, 589, 1433, 2002
TaqII	GACCGA	1 cut(s)	1269
TatI	WGTACW	2 cut(s)	487, 1109
TauI	GCSGC	1 cut(s)	1409
TfiI	GAWTC	7 cut(s)	91, 287, 332, 427, 461, 803, 896
Tru1I	TTAA	9 cut(s)	171, 399, 1151, 1155, 1307, 1728, 1775, 2117, 2181
Tru9I	TTAA	9 cut(s)	171, 399, 1151, 1155, 1307, 1728, 1775, 2117, 2181
TscAI	CASTG	4 cut(s)	355, 652, 851, 1200
TseFI	GTSAC	4 cut(s)	346, 604, 708, 1885
TseI	GCWGC	7 cut(s)	260, 392, 521, 931, 1316, 1639, 2041
Tsp45I	GTSAC	4 cut(s)	346, 604, 708, 1885
TspDTI	ATGAA	8 cut(s)	550, 643, 909, 1607, 1698, 1881, 1923, 2163
TspGWI	ACGGA	2 cut(s)	197, 671
TspRI	CASTG	4 cut(s)	355, 652, 851, 1200
Vha464I	CTTAAG	1 cut(s)	1774
VpaK11BI	GGWCC	2 cut(s)	357, 2011
XapI	RAATTY	5 cut(s)	1387, 1820, 1837, 1870, 1892
XbaI	TCTAGA	1 cut(s)	1069
XceI	RCATGY	1 cut(s)	1000
XspI	CTAG	5 cut(s)	320, 573, 633, 761, 1070
Zsp2I	ATGCAT	1 cut(s)	1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.